LOCUS       DACEND010000001        62243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEND010000001 DACEND010000000
VERSION     DACEND010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.001"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..289
                     /locus_tag="IW746_000001"
     CDS             <1..289
                     /locus_tag="IW746_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1076934.1"
                     /translation="LFSLNRRFKIKAPDLAIPFLWGGMHALSRNTGTISILPFFFISV
                     LLMGICIAVFQAYYYEEIIYPRYFKMTWRLVFLLTIVLYAVLIIVNILSYL"
     gene            406..891
                     /gene="mraZ"
                     /locus_tag="IW746_000002"
     CDS             406..891
                     /gene="mraZ"
                     /locus_tag="IW746_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1076935.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            912..1871
                     /gene="rsmH"
                     /locus_tag="IW746_000003"
     CDS             912..1871
                     /gene="rsmH"
                     /locus_tag="IW746_000003"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1076936.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1876..2277
                     /gene="ftsL"
                     /locus_tag="IW746_000004"
     CDS             1876..2277
                     /gene="ftsL"
                     /locus_tag="IW746_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1076937.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2278..4470
                     /locus_tag="IW746_000005"
     CDS             2278..4470
                     /locus_tag="IW746_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1076938.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4496..5458
                     /locus_tag="IW746_000006"
     CDS             4496..5458
                     /locus_tag="IW746_000006"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1076939.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5472..6833
                     /locus_tag="IW746_000007"
     CDS             5472..6833
                     /locus_tag="IW746_000007"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1076940.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6859..7947
                     /gene="murG"
                     /locus_tag="IW746_000008"
     CDS             6859..7947
                     /gene="murG"
                     /locus_tag="IW746_000008"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1076941.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7962..9182
                     /locus_tag="IW746_000009"
     CDS             7962..9182
                     /locus_tag="IW746_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1076942.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9338..10663
                     /gene="ftsA"
                     /locus_tag="IW746_000010"
     CDS             9338..10663
                     /gene="ftsA"
                     /locus_tag="IW746_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1076943.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10686..11927
                     /gene="ftsZ"
                     /locus_tag="IW746_000011"
     CDS             10686..11927
                     /gene="ftsZ"
                     /locus_tag="IW746_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1076944.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEIGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11944..12621
                     /locus_tag="IW746_000012"
     CDS             11944..12621
                     /locus_tag="IW746_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1076945.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12635..13234
                     /locus_tag="IW746_000013"
     CDS             12635..13234
                     /locus_tag="IW746_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1076946.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13249..13536
                     /locus_tag="IW746_000014"
     CDS             13249..13536
                     /locus_tag="IW746_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1076947.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13585..14367
                     /locus_tag="IW746_000015"
     CDS             13585..14367
                     /locus_tag="IW746_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1076948.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14388..15095
                     /locus_tag="IW746_000016"
     CDS             14388..15095
                     /locus_tag="IW746_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1076949.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15357..18155
                     /gene="ileS"
                     /locus_tag="IW746_000017"
     CDS             15357..18155
                     /gene="ileS"
                     /locus_tag="IW746_000017"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1076950.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18204..19730)
                     /locus_tag="IW746_000018"
     CDS             complement(18204..19730)
                     /locus_tag="IW746_000018"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1076951.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19745..20392)
                     /locus_tag="IW746_000019"
     CDS             complement(19745..20392)
                     /locus_tag="IW746_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1076952.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20576..20905)
                     /locus_tag="IW746_000020"
     CDS             complement(20576..20905)
                     /locus_tag="IW746_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1076953.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21082..21810
                     /gene="sfsA"
                     /locus_tag="IW746_000021"
     CDS             21082..21810
                     /gene="sfsA"
                     /locus_tag="IW746_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1076954.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21826..22839
                     /locus_tag="IW746_000022"
     CDS             21826..22839
                     /locus_tag="IW746_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1076955.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22839..24116
                     /locus_tag="IW746_000023"
     CDS             22839..24116
                     /locus_tag="IW746_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1076956.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24179..26881
                     /locus_tag="IW746_000024"
     CDS             24179..26881
                     /locus_tag="IW746_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1076957.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27033..27350
                     /locus_tag="IW746_000025"
     CDS             27033..27350
                     /locus_tag="IW746_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1076958.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27380..27700
                     /locus_tag="IW746_000026"
     CDS             27380..27700
                     /locus_tag="IW746_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1076959.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27808..29268
                     /locus_tag="IW746_000027"
     CDS             27808..29268
                     /locus_tag="IW746_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1076960.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29336..29557
                     /locus_tag="IW746_000028"
     CDS             29336..29557
                     /locus_tag="IW746_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076961.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29588..29770
                     /locus_tag="IW746_000029"
     CDS             29588..29770
                     /locus_tag="IW746_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1076962.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29770..30183
                     /locus_tag="IW746_000030"
     CDS             29770..30183
                     /locus_tag="IW746_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1076963.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30306..31487
                     /locus_tag="IW746_000031"
     CDS             30306..31487
                     /locus_tag="IW746_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1076964.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            31876..31949
                     /locus_tag="IW746_000032"
     tRNA            31876..31949
                     /locus_tag="IW746_000032"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31910..31912,aa:Arg,seq:cct)
     gene            complement(32018..33157)
                     /locus_tag="IW746_000033"
     CDS             complement(32018..33157)
                     /locus_tag="IW746_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1076965.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(33456..34091)
                     /locus_tag="IW746_000034"
     CDS             complement(33456..34091)
                     /locus_tag="IW746_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076966.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(34204..34839)
                     /locus_tag="IW746_000035"
     CDS             complement(34204..34839)
                     /locus_tag="IW746_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1076967.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(34873..35334)
                     /locus_tag="IW746_000036"
     CDS             complement(34873..35334)
                     /locus_tag="IW746_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1076968.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(35464..35895)
                     /locus_tag="IW746_000037"
     CDS             complement(35464..35895)
                     /locus_tag="IW746_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1076969.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(35913..36233)
                     /locus_tag="IW746_000038"
     CDS             complement(35913..36233)
                     /locus_tag="IW746_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1076970.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            36532..37308
                     /locus_tag="IW746_000039"
     CDS             36532..37308
                     /locus_tag="IW746_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1076971.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            37323..37526
                     /locus_tag="IW746_000040"
     CDS             37323..37526
                     /locus_tag="IW746_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1076972.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            37542..37880
                     /locus_tag="IW746_000041"
     CDS             37542..37880
                     /locus_tag="IW746_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1076973.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            37867..38046
                     /locus_tag="IW746_000042"
     CDS             37867..38046
                     /locus_tag="IW746_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076974.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(38089..38559)
                     /locus_tag="IW746_000043"
     CDS             complement(38089..38559)
                     /locus_tag="IW746_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076975.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            38646..39344
                     /locus_tag="IW746_000044"
     CDS             38646..39344
                     /locus_tag="IW746_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1076976.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            39522..39863
                     /locus_tag="IW746_000045"
     CDS             39522..39863
                     /locus_tag="IW746_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076977.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            39856..40527
                     /locus_tag="IW746_000046"
     CDS             39856..40527
                     /locus_tag="IW746_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1076978.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            40533..41219
                     /locus_tag="IW746_000047"
     CDS             40533..41219
                     /locus_tag="IW746_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076979.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            41222..41971
                     /locus_tag="IW746_000048"
     CDS             41222..41971
                     /locus_tag="IW746_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1076980.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            41983..42252
                     /locus_tag="IW746_000049"
     CDS             41983..42252
                     /locus_tag="IW746_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076981.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            42415..42714
                     /locus_tag="IW746_000050"
     CDS             42415..42714
                     /locus_tag="IW746_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076982.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            42714..43016
                     /locus_tag="IW746_000051"
     CDS             42714..43016
                     /locus_tag="IW746_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076983.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            43056..43421
                     /locus_tag="IW746_000052"
     CDS             43056..43421
                     /locus_tag="IW746_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076984.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            44048..44236
                     /locus_tag="IW746_000053"
     CDS             44048..44236
                     /locus_tag="IW746_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076985.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            44244..44438
                     /locus_tag="IW746_000054"
     CDS             44244..44438
                     /locus_tag="IW746_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076986.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(44471..44683)
                     /locus_tag="IW746_000055"
     CDS             complement(44471..44683)
                     /locus_tag="IW746_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076987.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            44808..45011
                     /locus_tag="IW746_000056"
     CDS             44808..45011
                     /locus_tag="IW746_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076988.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            45008..45280
                     /locus_tag="IW746_000057"
     CDS             45008..45280
                     /locus_tag="IW746_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1076989.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            45281..45466
                     /locus_tag="IW746_000058"
     CDS             45281..45466
                     /locus_tag="IW746_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076990.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            45453..45800
                     /locus_tag="IW746_000059"
     CDS             45453..45800
                     /locus_tag="IW746_000059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076991.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            45760..46236
                     /locus_tag="IW746_000060"
     CDS             45760..46236
                     /locus_tag="IW746_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1076992.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(46382..47110)
                     /locus_tag="IW746_000061"
     CDS             complement(46382..47110)
                     /locus_tag="IW746_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1076993.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            47158..47436
                     /locus_tag="IW746_000062"
     CDS             47158..47436
                     /locus_tag="IW746_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076994.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            47438..47824
                     /locus_tag="IW746_000063"
     CDS             47438..47824
                     /locus_tag="IW746_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1076995.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            47821..48201
                     /locus_tag="IW746_000064"
     CDS             47821..48201
                     /locus_tag="IW746_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1076996.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            48313..48765
                     /locus_tag="IW746_000065"
     CDS             48313..48765
                     /locus_tag="IW746_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1076997.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            48762..50489
                     /locus_tag="IW746_000066"
     CDS             48762..50489
                     /locus_tag="IW746_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1076998.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            50553..51740
                     /locus_tag="IW746_000067"
     CDS             50553..51740
                     /locus_tag="IW746_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1076999.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            51706..52281
                     /locus_tag="IW746_000068"
     CDS             51706..52281
                     /locus_tag="IW746_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1077000.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            52294..53658
                     /locus_tag="IW746_000069"
     CDS             52294..53658
                     /locus_tag="IW746_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1077001.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            53660..53941
                     /locus_tag="IW746_000070"
     CDS             53660..53941
                     /locus_tag="IW746_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1077002.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            53919..54245
                     /locus_tag="IW746_000071"
     CDS             53919..54245
                     /locus_tag="IW746_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1077003.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            54235..54573
                     /locus_tag="IW746_000072"
     CDS             54235..54573
                     /locus_tag="IW746_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077004.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            54563..54928
                     /locus_tag="IW746_000073"
     CDS             54563..54928
                     /locus_tag="IW746_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1077005.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            54935..55561
                     /locus_tag="IW746_000074"
     CDS             54935..55561
                     /locus_tag="IW746_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1077006.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            55561..56025
                     /locus_tag="IW746_000075"
     CDS             55561..56025
                     /locus_tag="IW746_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077007.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            56220..58529
                     /locus_tag="IW746_000076"
     CDS             56220..58529
                     /locus_tag="IW746_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1077008.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            58541..59245
                     /locus_tag="IW746_000077"
     CDS             58541..59245
                     /locus_tag="IW746_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1077009.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            59242..62001
                     /locus_tag="IW746_000078"
     CDS             59242..62001
                     /locus_tag="IW746_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1077010.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            62014..>62243
                     /locus_tag="IW746_000079"
     CDS             62014..>62243
                     /locus_tag="IW746_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1077011.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        21261 a        10214 c        12892 g        17876 t
ORIGIN      
        1 attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt
       61 gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt
      121 ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga
      181 agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat
      241 cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa
      301 aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg
      361 aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt
      421 agactaattt tagttagtgg aggaatgggg gggctttcaa tgttgatggg tgaattccaa
      481 cataatattg atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga agaacttggc
      541 gaaaagtttg ttctgacacg cggattggat ggctgtcttt tcggctatcc gatgagcgaa
      601 tgggaaaatc tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga cgcacgtact
      661 tttgtgcgat tcttctattc ggctgccact gaatgcgaac ttgataaaca aggacgaatc
      721 aatataccca gcacgttaag aaattatgca gctttgacaa aaggttgcgt agtcatcggt
      781 gtatctaacc gaattgaaat ttgggatgag acacgatggc aagatttttc tgcagcagca
      841 gaagagaact ttgacgaaat tgctgaaagt atgattgatt tcggactata agaataggaa
      901 gaggctgact aatgactgaa acatttcaac actatacagt catgttgaaa gaaacggtag
      961 atggattgaa catcaaaccg gatggcgttt atgtagactg tacactcggc ggtgccggac
     1021 atagtgaata tttattaagc caactaagtt ctaaagggca tctgtatgcg tttgaccaag
     1081 atcaaaaagc aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa caaggtatgg
     1141 tcacatttat caaagcgaac ttcagagaat tagaagaacg cttgagtgag catgtagata
     1201 aggtagacgg cattttgtat gatctagggg tttcctctcc tcagctagat gaagcagaac
     1261 gtggatttag ttatcatcaa gatgcaccgt tggatatgcg catggatcag tcagctcctt
     1321 tatccgctta tcacgtaatt aatgaatatt cctaccatga attagtaaag attttcttcc
     1381 gctatggcga ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga gtacgaaaaa
     1441 caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc atcccagctc
     1501 ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg cggattgcag
     1561 taaatgacga actcggtgcc gaagaggcct ctttagaaca ggcaatccgg ttattgaaag
     1621 tcaatggaag aatcagcgta atcacattcc attcattgga agacagaatt gtcaaaagta
     1681 tgtttaaaga atatagtacg gtacaagatc taccaccggg acttcctgtc atacctgacg
     1741 aattccaacc agaactgaaa gtcattactc gtaaaccaat ccttccttca gaggaagagc
     1801 taacagaaaa caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa ataagaatga
     1861 aagaggagta gaatgatggc agaattaaag aaaatgcaag attacgaata tgatttacct
     1921 gaattggtgg atcagccgga acaacaaatg cctgattctt cttcgaatca agaaatcatt
     1981 cttccacaat caccggcaaa acgattaaag cagatttctg ctttggaaaa aattgctgtg
     2041 gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa cagttatgct gcgcacaaat
     2101 atcagcggag ctgaaaaaga aatcacgtca atacaaacag aaatcaatga taaaaagcaa
     2161 gaaaaaacga gtttagaaca agaaaaaaac gaactttctc gtactgaacg gattaaacaa
     2221 atcgcagaag aaaaaggatt gtctattaat gacgacaatt taaggaaagt gaagtaaatg
     2281 agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc gataaacaat
     2341 cgcaaaaaag taggtatcat tttatttgct acgagtattg gattgttctt tttatttgcc
     2401 gtccgtttct cgtatatcgt tattggaggt catgttgctg gtacctcatt agctgaaaaa
     2461 acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac gatttatgat
     2521 cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt caattaaagc gatactctca
     2581 aaaacatata cctctgggga taaaaaactg tatgttgaag aaaagaattt tgataaaata
     2641 gcagaaatcc tgcataagaa tctttctata gacaaaaaag atgcactgaa tattttagag
     2701 gacggcgcaa aaaaagagct gtatcaagtt gaatttggta gctatgggaa aaatatctcc
     2761 caagaaacca agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc tggtctttat
     2821 tttgtcgacc atcaggctcg gatgtatcca aatggcgtct tttcttctca ttttattgga
     2881 tacgcagttc cagacaaaga tgagaatggc ttagttggta aactaggttt ggagtctgct
     2941 tacaatgata tcttgagcgg taaagatggt aagattattt atcaaaaaga taattttcaa
     3001 aatcctctgc caggtacagt ggcagaagaa gaaaaagctg tcgacggaca agatatctac
     3061 acgactttag acagcagact acaaagttat ttagaaacac tgatggatca agtaaatgaa
     3121 gaatatcagc cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg agaaatcttg
     3181 gcaatgggac aacgacctac tttcaatcct gaaacaatgg aaggattgac tggaaaagat
     3241 gctatctggc gtaatttctt ggtacaagat agttatgaac caggatcaac catgaaagtt
     3301 ttcactactg cagctgctat cgaagaagga gaattcaacg agaatgaaac cttccaatct
     3361 ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg actttggtga aaaaggtgtg
     3421 ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg ggatggtcat actggaacag
     3481 cgattaggcg gacgatggta taactattta caaaaactgg gatttggaca aagtactcat
     3541 tctggattag atgatgaagt aaacggtgct ttacctacat taaatattgt cgaccgagca
     3601 atgagtgctt atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa agctttcact
     3661 tctattgcga ataacggtac aatgattcaa cctcgatata tcagcaaagt ggtggatccc
     3721 caaactggag aagagcgaac aactcaaaca gaagttctag ggcaaccttt ttcaaaagaa
     3781 acaacagaaa aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa ctacggtagt
     3841 gcttatggtg tatatagtgt acctggatac aatgtatcgg caaaaaccgg aacagcccaa
     3901 atcgcttctg atacaggggg atatcaaaca ggagatacag cctacctgta ttcgattgtg
     3961 gagatggttc cttctgaaga tccagactat gtcttgtatc taacaatgaa acaccctaag
     4021 acatatgatc gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg ggcaatggat
     4081 tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt atccgtagcc
     4141 gattatcgta atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag tggactgcaa
     4201 cctgtagtga tcggtaatgg taaaaaagta caaaaacaat ctactgccaa cggtgatcag
     4261 ttgatctctg gagaaaaact gatcctatat actggtggtg ataagctgat gcctgacgta
     4321 acaggatggt caaaagcaga tatcatgaag ctggggaaaa tcctcggtat tgaagttagt
     4381 tttgatggag atggttattg tgtcaaacaa gaacttgctc catatgaaaa aatcactgaa
     4441 gacaaactta gttttacttt agaagaataa gaagagaaat aggagagaaa agagcatgga
     4501 atggacacaa gcattgattc ccattgtcag cagttgtgca atgacaattg ccgcaatgcc
     4561 gctatttatt ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg aggagggacc
     4621 aaagtggcac aatagcaaag ccggaactcc tacaatgggc ggcttagtgt ttctgatcgg
     4681 ctctattttg acaggaattt gggttggagc ttggcaaaaa cagctaactc ctactctttt
     4741 catcttgctt tttgtattag cgttatacgg agtaatcggc tttcttgatg attttataaa
     4801 aatattcaag aaaagaaaca tgggactgaa ctcgaagcag aaattgattg gacagattct
     4861 agggggaatc atcttttatc ttgtttaccg ttctgaaggt tatccaggtg tattgaattt
     4921 cttcggacta gatctacctt taggaatcat ttatggtatt ttcgctctct tctggcttgt
     4981 tggattttca aatgcagtaa atctgacaga tggcatcgat ggattagttg ctggcttagg
     5041 aagtatctct tttgcagctt acggtctaat tgcttggcat cagcatcagt atgatgtatt
     5101 aatcatttgc ttaagtgttt taggaggttt actgggtttc ttcgtctaca acagaaaacc
     5161 tgccaagatt tttatgggag atgtcggttc attagcgttg ggcggacttt tagcggcaat
     5221 ttctataatg ctaaaccaag aatggacatt gcttttgatt ggtttgatct atgttatgga
     5281 aacagcaagt gtgatgcttc aagttacttc ctttaagctt acaggaaaac gaatttttaa
     5341 aatgtctcca attcatcatc actttgaaat gtgcggatgg tcggagtgga agattgatac
     5401 agttttttgg ttgatcagca ttgtgacttc gttgatcaca ttatggatcg tttggtagga
     5461 gatgacggga aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt gtcttaggac
     5521 tcgcaaaaag cggagtaagt gcggccaaat tattacatga attgggcgca cttgtcaccg
     5581 ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca agagctgctt tctttaggga
     5641 taaaagtaat aacaggtagt catccaatcg aattgttgga tgaagaattt tcattgatgg
     5701 tcaaaaatcc agggattccc tacagtcatc ctttagtagc aaaagcccaa gaaatgggta
     5761 ttccggtaat tacggaagtt gagctagctt acgaagtagc ggaatgccca attattggga
     5821 ttacaggaac gaacggaaaa acaacgacaa caacgatgac aggactacta ctgaatgcag
     5881 gagcagatca aggaattgca cgtttagcgg gtaatattgg gtatccagca agtggtgtag
     5941 cgcaagaagc aaaatcagaa gataaaattg tgatggaatt gtctagcttc caactcatgg
     6001 gaatcacaga tttccgccct catatagcgg tgattaccaa tatctatgaa gcacatattg
     6061 attatcatgg tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa aaaaacatga
     6121 cagaaaaaga ttatctgatc cttaactgga atcagtcaga actacaagaa ttagcgcaaa
     6181 gaacaaaagc acgcgtactg cctttctcga caaaagaagt gttggaagac ggtgtgtatg
     6241 ctgacgatta ctcaatatac tataaaaaag aaaaaataat ggaaatatct gaacttggcg
     6301 taccaggtaa gcataatgta gaaaatgcac tagctgctat ttctgtagca aaattgtatg
     6361 gtatatcaaa tgaagctata cgagaaacgt tgcatttttt ccatggcgta ccacatcgta
     6421 cgcaatatgt aggagaaatc caaggcagaa aattttataa tgattcaaaa gcaaccaata
     6481 ttttagcgac taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg ttattggctg
     6541 gcggattaga cagaggaaat acatttgatg aattaattcc ttcattaaaa ggaatcaaag
     6601 caatggtcgt atttggccaa acaaaagaaa agttgatgga tgctggaaaa aaagcaggca
     6661 tcgagacaat agtaacagcc gattctgtag aacaagcagt tcctttagca ttagaaaaca
     6721 gtacagatgg tgatgtagtt ttattatcac cagcgaatgc cagctgggat caatacccaa
     6781 attttgaaac tagaggaaac agattcatgg aagctgtgaa ccgattaaaa tagaaaaagt
     6841 agaagcgagc gataaaaaat gaaaattttg gttacaggcg gaggaacagg gggacatatt
     6901 tatccagctc ttgcctttgt caattatgta aagactcaag aaccgaatgc tgaattcatg
     6961 tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg aatgcctttc
     7021 cataccttgg agattcaagg atttaaacga aaaatttcca tgcataacat caagacggtc
     7081 cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt tcaaccagat
     7141 atcgttatcg gtactggagg atatgtttcc ggagcggtag tgtacgctgc agccaaaatg
     7201 ggtattccaa cagttatcca tgaacaaaac agtgtaccag gtgttaccaa taaattttta
     7261 agccgatatg tggatcggat cgctctttca tttaaagatg cggcttcctt ttttcctgaa
     7321 aacaaagcag ttcttgtagg taatcctcga gcacaagaag tagcagatac taaaaaatca
     7381 gatattttga aaacatttga tttggatcca gaaaagaaaa cagtcttgat ttttggcggt
     7441 agtcaaggag cacttaaaat caatcaagca gtatcagttt ttttaagtga atatattcct
     7501 tcttcttacc aagttttgta tgcttcagga gatagatatt atgaagagat cctaaataaa
     7561 ataaatcctg aaatacaaaa tattagtgtc cagccttata tcaaaaatat ggcagaggtc
     7621 atggcttcca gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc agaattcaca
     7681 gctctggggt taccggcagt gttggttccc agcccatatg ttacgaatga tcatcagact
     7741 aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga taatgaacta
     7801 acaggtgaat cactaagtca gacagtcaac gaaatcatgg gtgatgaaga attgcaaaag
     7861 caaatgtgcc gagcttctaa agaacaagga atccctgatg cttcaaaacg tctttatgac
     7921 ttagtaaaac aaataatcaa aaaataaagg cggtggtctt agtgagtaag aaaaaagaac
     7981 caaaaaaatt gtctcatccg tcttctgatg aaaacaattt gactccttgg caaaaggcta
     8041 accgaaaata tctggcagaa caagagatga aaaaccaaga gccaaaaaaa gaagacataa
     8101 cattagatac acccctttca gattctgaag aagaaaaaac ggttcaagag gaacagcaag
     8161 aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa aagaaaaacg
     8221 gtggaccata taacggttct tttttgaacc ggctgcccaa tttaaaaagt caacggcaca
     8281 aagtactatt tcgtcggtta gctttgatca tcactgtatt gggtataccg ctgatttttt
     8341 tgatctatta tgtttctcct tatagcaaat tacaagcagt tgcagtatca ggcaataaga
     8401 cagtcaacag ccaagaaatc atctctgata caaaattgtc attaggagaa aatgtttggg
     8461 gacagtattt tcatcgatct acttatatcg atcgccttaa aaaagcacag ccaagaatcg
     8521 aaacagcaaa tatacatttc aaagggatga atgaattcga tttggatgta acagaatata
     8581 aagagattgc tttgatcgca aaaaacaatc aatactaccc agtgattgaa aatggaacag
     8641 tattagatga aaaagtagca aatcctacaa aaaatctccc tatcttggaa gagttcaaag
     8701 actctgcaaa aatcaaagaa ttaacaaagc aatacaatca gttatcatca gaattacaaa
     8761 aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg atccaattga
     8821 atatgaatga cggcaatcaa gttattgtaa atatcaataa tcttgcgaac caaatgaaat
     8881 attattcgca agtagccaaa gatatggatg aaaaaggtgt gatcgacatg gaagttggta
     8941 tcttctcgca tccttacggt agcactcaag aaacggaaga atcgggccaa tcggaagaaa
     9001 caactgaaag tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag gcagaaacgc
     9061 aaaaagcaca aacacctgga tcgtctagca caaacggatc atctgattca aatgattcaa
     9121 gtgaaacaag tcaaatagaa gataatccaa gtacaactcc gtcaagttta aatgaaaatt
     9181 gataaaacaa ctttaaaaag taagaatgta ctttctaaaa accagtaatt tatggtagaa
     9241 ttggaagcag tgaatatatc tattaataag tatattttaa aacggacgct agttgttcgc
     9301 ttgtatatgg aatagtaagt aggagggaat cccattcatg gcaaaaacag gaatgtatgt
     9361 aggccttgat attggaacga catctgttaa agttgtcgtg gctgaatata tcgacagcca
     9421 aatgaatatt attggagtag gaaacgcaaa atcagaaggg attaaccgag gcattatcgt
     9481 tgatatcgat aaaacggtcc aagcgataca acgagcagta cgacaagcag aagaaaaagc
     9541 aggtatccag atcaaaggcg taagtgtcgg tttgccagca aacatgcttg aagtagaaaa
     9601 ttgccaggga atgattgcag ttaacggaga ttcaaaggaa atcaccgatg aagatgtcag
     9661 aaatgtggct tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa tcgtgtcaat
     9721 attgccacaa gattttactg tcgatgggtt cgagggaatc aaagatcctc gtggaatgat
     9781 cggcgtaaga ttagaaatgt acggtctgtt gtttacagga cctaaaacga tcatccataa
     9841 catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga ttactccttt
     9901 agcgctgact gaatcaattc tttctgacgg agaaaaagat tttggtacga tcgtcattga
     9961 tatgggtggc ggccaaacaa caacggctgt tatgcatgat aagcaattga agtttaccaa
    10021 tcttgaccaa gaaggtggcg aatttgtcac taaagatatc tctattgtat taaatacgtc
    10081 atttaacaat gcagaagcgt tgaaaataaa ctacggggat gcatacccag aacggacatc
    10141 accagatgaa gaatttccag tagatgtgat tggtcagtca gaaccagtga aagtcgatga
    10201 acgctacttg tcagaaatta tttcagctcg aatggaacag attttcaaca aagcaaaaga
    10261 tgcattggac caaatcgaag cattagagtt gccaggtggc gtcgtattga caggcggagc
    10321 tgctagtctt cctggtgttg tggacttggc acaagaaatc ttcggcgtaa atgtaaaatt
    10381 gtatgttcca aatcacatgg ggctgagaaa cccggtattt acaaatgtaa taagtatcgt
    10441 ggattattca gcaaacttga gtgaagtgta tcaattggca aaaattgctg ttacgggtga
    10501 aacctcagca gctcgtcaaa tggtagttga acaagaaaca acaaacacat atgagagcta
    10561 tgaagcacct gaagaaacaa tctacgatga gccggaacca aaagagtccg gagaaaatgt
    10621 aaaaaataaa atcaagggtt tctttacaaa tatttttgat taattcatcc tgggaggaat
    10681 aagatatgga attctcaatt gataataata tcaatgacgg cgcagtaatc aaagtcatcg
    10741 gagtcggtgg tggcggtgga aatgctgtaa accgaatgat tgaagaaaac gttaaaggtg
    10801 ttgaattcat tacagccaac acagacgtgc aagcattaaa aaattcaaaa gctgaaacag
    10861 ttatccaatt aggccccaaa tatactcgtg gtttaggtgc tggttctcag ccagaagttg
    10921 gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga agctttagac ggtgcagata
    10981 tgattttcat cactgcagga atgggtggag gaaccggtac aggtgctgca ccaatcgttg
    11041 caggtatcgc gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt ccattcactt
    11101 ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg aaagaaaatg
    11161 tggatacatt attaattatc tcaaataacc gtttattaga agttgtagat aaaaagacac
    11221 cgatgcttga agctttccgt gaagcagata atgttttacg acaaggtgta caaggtatct
    11281 ctgatttgat cacagcgcca ggttacgtta accttgactt tgctgacgtg aaaacagtga
    11341 tggaaaacca aggtacagca ttgatgggga tcggtgtggc tagtggagaa gatcgcgtag
    11401 tagaagcaac gaaaaaagca atctcctctc cattgcttga aacatctatt gatggagctg
    11461 aacaagtact tcttaacatc acaggcggtt tggatatgac attgttcgaa gcacaagatg
    11521 cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta ggtacatcta
    11581 tcaatgaaga aataggcgat gaaatccgtg tgacggtcat cgcaacaggt atcgatgaat
    11641 caaaaaagga acgcaagtct agccgaccag ctagacaagc acaaatgcaa tcacctgctc
    11701 aaaagactgt attagatatg gatcaagcta aaccaacttc ttcagaagaa gaaaacagtt
    11761 ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat gattccaatt
    11821 ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca aaatctcatg
    11881 gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg agaggaagag
    11941 cacatgattg ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc gtgtgctctt
    12001 gttcatcgtg atccgaaaga agttacatta gtagctgtaa caaaatctgt tgaatccgct
    12061 caagctgaag aattggttag agctggcgtt atgaattgtg cagaaaaccg agtagataaa
    12121 ttattagaaa agaaacaaga tttatttgat tttcctgaaa taaagtggca tttaattggt
    12181 aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt tcatgcgttg
    12241 gacagtttaa gattagcaga agaaatccag aagcggttag agaaagaatt atcctgtttt
    12301 attgaagtaa atgtttctgg tgaggaatca aaacatggaa tttcaccaga tgagctttta
    12361 ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt gatgactatg
    12421 gcgcctaaag aagcttctga tgagcaaatc cataaagtgt ttggtacgct gaaagaattg
    12481 cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt aagtatgggg
    12541 atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa catttatccg tgtaggaaca
    12601 gcgctattca aagacgaata aaggaggaac aaaaatgccg cttttaaaca gaggagcaat
    12661 tgctaatttt ttcggattag ctgatgagga agagtacgaa taccaagatt atcctgaaac
    12721 aacaaaaaag gctgtacagc caactaaaaa aaatacaaca cagaatcaat caactcattc
    12781 tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc ctgtaacgca
    12841 tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa ctacatcaat cacctacaaa
    12901 aactgtagcg aaagaaagaa cagcttcaag aacctctaca caacctaaag caaatcttgc
    12961 tggaaaaatc actgtaatgg aaccaagagc ttattctgaa gctatggcga ttgctaaacg
    13021 aatcattgct ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa aacaagcgag
    13081 aagaatcgtt gattttctaa caggaacagt ttatgcacta gatggagata tcaaacgtgt
    13141 tggagatgag atttttcttt gtacaccgac tgggatggag attgacagtt caaccgctca
    13201 atcttttgca gatgcgaatt tgtttgatct gtaagaggag gaactacgat ttatactatt
    13261 gcactattac ttcatttact tcaacgggca gcctatttct atagcattat cttgatggta
    13321 tatgcattgc tctcttggtt tccaggcggg tatcaatcag ggctaggacg attattagct
    13381 aagatctgcg aaccgtatat cagtttgttc gatcgtctgc ctttgaggat tggacccttg
    13441 gatttcacaa ttttaggggc aatcctgatc ttgaacttag ccgtacaagg tttagcacag
    13501 atcgttatat ggataactgt ccatttagtt tattgattga agcaacaaaa tctttattct
    13561 cagaatagat aggaggggaa aattttggat gcaaatgtat accagcactt tcgggcagat
    13621 gaacgtacat ttatcgattc ggtaggcgat tggattgaac aggttcaagg acaatatgca
    13681 ccctatctga ccgaatttct tgacccaaga caatcctata ttttagaaac actgattcga
    13741 caaagcagtg atttgcgttt catgttttat ggcggatatg aacaggcaga aagaaaaaga
    13801 tgtttgattt tccccgatta ttacgaacct gtagaggaag attttgaaac agcattgtat
    13861 gaaatccatt atcctagcaa atttgccagc ctaagtcatg ggaaaatcct tggaacttta
    13921 gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca tctcggacgg tgatcgctgg
    13981 caagtattta ttgcaaatga ggtggatcat tttgttgctg cacaagtaac aaagattgga
    14041 aaggttgctg tacgcttgga accaatcaaa tacacagaaa tgattatgcc gaaagatagc
    14101 tggacacaag aaagaggaac agttagttcc atgcgactag acagtgtgat ctctgagatt
    14161 tataatattt ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt aaaagtgaac
    14221 tggacagaaa atacaagacc tgattttgta cttgaactat tagatattat ttcagtcaga
    14281 ggttatggca gagtccagat ccaagaactt gaggggaaga cgaaaaaaga aaagtacagg
    14341 gtactgttcg gtgtattacg taaataaaat aaaaaggtgg tttactcatg gctttaactc
    14401 cattagatat ccaaaataag acattcccga caaaaatgcg gggatataat caagacgaag
    14461 tagatgattt cttagattta gttgttcgtg actatgagga attgactcaa agaaatcgag
    14521 aattagaaaa agcagtcaaa cattctgaag aaaaacttga gtacttcaat gaattaaaag
    14581 atgctttgaa ccaatcaatc atcgtagctc aagatacggc tgataaagta aaaacaagtg
    14641 caagcaagga atccgaagtg atcgttactt ctgcacaaaa caaagcagat gagttagttg
    14701 cgaacgctga aaaacgtgca catcaattaa caactgatgc agaagaaaaa gcaagaaaaa
    14761 tcttgacaga tgcaacagaa aaagcaagac agcttgcgac agaaactgaa gatcttaaga
    14821 aaaaaacacg agtattccat caacgtatca gcttgatgct tgagtctcaa ttagaacaag
    14881 tgaaaagtcc ggaatgggat gaaattttac aaccattctc cagctacgtg actgacagcc
    14941 atgaagtgat caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat actgatgtaa
    15001 actcagacac aggagtcata gaagcaccta tgacggcatt tgatacacag gctattgata
    15061 tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag aaaagcactt tgttattctt
    15121 aaagcgagcc gatgagagtg gaagttcggt aagacactgc atggtgaaag atcctctact
    15181 tgagacctgc ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt attggtcaca
    15241 gagagtttgc actagataga tgcaactaaa tttgggtggt accacggcat gttcgtccct
    15301 tgcggctgaa catgcttttt tgtgtccgga aaacaaataa aggagtgtat ggttgaatga
    15361 aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt aatttgccaa
    15421 accgagaagg cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa aaaagacaag
    15481 agttgaacga aggaaaacct agctttgttc ttcatgatgg acctccatat gccaatggta
    15541 atatccattt aggacattca ttgaataaaa tcagtaaaga cattatcatt cgttcaaaat
    15601 caatgtctgg ctatcgtgca ccatatgttc caggatggga tacacatgga ttgccgattg
    15661 aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca gaatacttgg
    15721 aaaaatgtcg tgaatatgca ctttcccaag tagataagca acgagcagat ttcaaacgcc
    15781 taggagttgc aggagattgg gaacatcctt atgttacatt agatccttct tatgaagctg
    15841 cacaagtacg cgtatttggt aaaatggctg aaaaaggcta tatttacaaa ggattgaaac
    15901 caatttactg gtctccgtca agtgagtctt ctttagcgga agctgaaatt gaatataaag
    15961 atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa agacggcaaa gatattcttg
    16021 atgaagatac agcttttgtc atttggacga caacaccatg gacattgcca gcaaaccaag
    16081 gtattgctgt aaatccaaaa tttacgtatg tattggtgga agccgatggc cgtaagtttg
    16141 tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat cggttgggaa aatgtaacag
    16201 ttctaaaaga atttgctgga caagaaatgg aatatatgac tgcaagtcat ccattctatg
    16261 aacgtgaatc acttgtgatt ttaggcgatc atgttacttt agacgctggt actggattag
    16321 tccatactgc tccaggacat ggggaagatg actatatcgc tggtagcaaa tataaactat
    16381 ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga agaagcacct ggatttgaag
    16441 gtatctttta tgacaaagca aatcctatga tcactgatct attaaaagaa aaaggagctt
    16501 tgctgaagct agatttcttt actcatagtt atcctcacga ttggcggacg aaaaagcctg
    16561 tgatctatcg ggcaactcca caatggtttg catctatcga taaattccgt cagaatattt
    16621 tggatgaagt agaaaaagta gagtgggtta tcccatgggg taaaacacgt ttgtataata
    16681 tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga gtaccacttc
    16741 cgattttcta tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc gaacatgtgg
    16801 caaaattatt tgctgaacat ggatcaatta tctggtttga aaaagaagcg aaagaacttc
    16861 ttcctgaagg atttacccac ccaggttctc ctaatggcga atttacaaaa gaaaaagata
    16921 tcatggatgt ctggtttgat tctggttctt ctcatgaagc tgttttacgt cagcgaccag
    16981 aattatcatt cccagcagat atgtatttag aaggatcaga ccaatatcgt ggctggttca
    17041 actcaagtat cacgactagt gtggctatca atggtgtagc accatacaaa tcagttattt
    17101 ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag taaatcttta ggaaacatca
    17161 ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat ccttcgctta tgggtatcta
    17221 gtgtagatta tgaagcagat gttcgtgttt ctatggatat cttaagtcaa gtggcagaag
    17281 tatatcgtaa aatcagaaat acgatgcgtt tcttattagc gaatacagaa gatttcgatc
    17341 ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt agacaaatat atgaccgttc
    17401 gattgaacca agtaatcaaa gaaatccgag aagaagggta cgacaaatat aacttcatgc
    17461 atatttaccg tacagttatg aacttcttga cggttgatct atcgtcattc tacctggatt
    17521 ttgctaaaga tgtagtttat attgaagcag aagatgatta tcaacgtcgt tgcatgcaaa
    17581 ctgttttcta tcaaacagct gtagctttga caaaattatt gactcctatc attccgcata
    17641 cagcagaaga aatctggtca ttcctgaaag aagaagagga gtacgttcag ttaagcgagt
    17701 ttcctggtta tgaagagttt gcaaaccaag aagaattgat ggatacctgg actgctttca
    17761 tggacttccg tgataatgtc ttaaaagcat tggaagaagc aagaaacagc aaattgattg
    17821 gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt gaaatgttaa
    17881 aagctttgga tgcagatatt gcacaattat tgatcgtttc accagactac tttgaagtta
    17941 tggcagcaga tgaacaagtg ccagataacg ctatggtatt tgatgatgtt gctattacag
    18001 tggaaaaagc agacggggaa acttgcgatc gctgtcgcca agttcgtaaa gatgtaggag
    18061 tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa gacttctatc
    18121 ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt gtgacatgtt
    18181 ctttgtcaca cccccttttg ttatcaatcg tttaattttc cacgttcacg ataccagtta
    18241 tctggttgcc agatccattc gaatccgtct ttttcaagta gttcgaaagc tgctttaggt
    18301 cccattgatc cagctttgta gttgggaaaa tccggttgtg tttggtccca agcttttcga
    18361 atgacatcca cgatatgcca agattgagcg acttcttccc aatgagagaa gttcgttcca
    18421 tcaccattca acgcatctaa tagtaatttt tcgtaagctt ctgggctatt ttcaaccatt
    18481 tctgcactat ttctaaattc caatttgatt ggttccgttt caaatccttg ccctacttct
    18541 ttcccattca aggataaaga aaatccttca gttggctgaa tatagatcgt tagaacatta
    18601 ggtggaagtg tcgtatcgtc acatggttca tcgacagatg ttttgaatac attaacagga
    18661 acttgtttga atacgatgtt gatccgagtc cctttttcag tcaaacgttt gcctgtacgt
    18721 acatagaaag gaacacctga ccaacggaag ttatcgatag tgaatttgcc tgcgacgaat
    18781 gtttcagtct ctgattgagc atccactttg tcttcttcgc gatagccctt gaattgtaca
    18841 ccatccaatt caccttgagc atattgcccg cgaacgaagt tttctaacac ttcttcttcg
    18901 ttataaatgc ggatagcttt caatgctttg atcttttctg tacggatttc tttttcggaa
    18961 aatgctactg gcggttccat tgcaagtaat gctacaacct gtaaaatatg attttgcacc
    19021 atgtctttaa gtgcgccact atgatcgtag tagcccccgc gatcttctac accgaggttt
    19081 tcgccaaaag taatttggat attgtcgata tagcgattgt tccattgagc ctcaaaaata
    19141 ttattggcaa aacggatagc tgagatgttt tggatcattt cttttcctaa ataatgatca
    19201 atcctgaaaa tatcttgttc tgggaatact tctctgattt gctggttcaa ttcaaaagca
    19261 gactggtaat cagaaccgaa cggtttttct atgatcaaac gattgaagcc attttttgtc
    19321 ataatatttt gtgatttcaa atgagaaacg atcgttccaa aaaattgagg tgccatagcc
    19381 aaataataga cacggttacc gtttaattga tagtgtccat ctaatcggtc agacaattct
    19441 tttaatgtct gataatgcgt tgaatcattt acatcatggg attgatagta aaaatggctt
    19501 gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac ggattgtttc tttgacaact
    19561 tcacggtagt gctcatcgct ccagggacgg cgagcggttc cgattacagc aaaatgctcg
    19621 cctaaatttc cttttctgta aaggcggaac aaagacgggt acaatttacg ctgggctagg
    19681 tcacctgtac cgccgaatat cgtaaacaat acattttttt cattagacat gtggatacac
    19741 tcctttatat tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg gggattttct
    19801 ttctatcatt ttggatagtg atcggtcctt cataagctgc gatgtcaacg atctcatatt
    19861 cttcgttgat attcaggtca atagtgacta agtaatccaa caattctttt tcatcaagca
    19921 ctcgtgcgat acgcactttt gtaccgacag gataggactc caatgtttgt cggcgttctt
    19981 catggacaac ttcctctttt ttaggaatcg atcctccatg aggacaatgt tcaggatgat
    20041 tcaaatagtt ttctaaatga tctgcaagat gttccgaagt cacatgttcc aatacttcag
    20101 catcatcatg gacttcattc cagctatagt ctaaatgttc aactaaaaaa acttcccata
    20161 agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc aattgtacac
    20221 cttgataagg ggaatgttct acaagctctt ctttcacaag tttagaaatc atttcactga
    20281 cagatgcagc tgagacatct agactagata cgatttgttt attgttgact ttcgttgtat
    20341 ctccacctag ttctaaaatg attttcaaat aatcttctcg atttggtgtc atattcgatc
    20401 atcctttcat ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt taattgacta
    20461 tttttctaaa taaaaaacaa aggattttta gacgttttta caacaaaaaa cgaaagatga
    20521 tagagaagac tcattctttc gtttataaag gatatatata tacattggga gggatctatt
    20581 tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt tgttcgggat
    20641 aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat tcatctattt
    20701 ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca gatgtaatct
    20761 gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac tttgagagtc
    20821 cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag agaacggtaa
    20881 tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt tatgagaaaa
    20941 taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa gctaaaattt
    21001 ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata ggatacatag
    21061 taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt ttttattgac
    21121 cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat tattgcgcat
    21181 gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt ggctattgta
    21241 tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa aaagaagggt
    21301 gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg tattttagac
    21361 ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg tgaagtaact
    21421 tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa agcatttgtt
    21481 gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga tgcgcccaca
    21541 ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg ttatgccgct
    21601 tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca tgaagaaatg
    21661 caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca aatcctcgcc
    21721 tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc atttgatgta
    21781 gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat caacttaggt
    21841 attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag cacaaaaaaa
    21901 tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt tggttctcat
    21961 tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat tactcatatg
    22021 gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa gcaagcaatt
    22081 ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa agaaatggat
    22141 gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc aagaaatatc
    22201 catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga gataatcggt
    22261 gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga tggagaagaa
    22321 ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg tgtttacctt
    22381 gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt cccaagaaag
    22441 atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa ttttgatgta
    22501 tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta ttttgactca
    22561 ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca tgatcgcgcg
    22621 cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc agatgaagca
    22681 gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt gttgtatgaa
    22741 gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag aaaaaatggc
    22801 ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt gtacaagtat
    22861 ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct tctcttattc
    22921 aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt tctcctacag
    22981 gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa ccaggtgaag
    23041 caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta gcagatgttg
    23101 cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc ggagccaacg
    23161 tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt acaccaggaa
    23221 gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa acaatggtat
    23281 ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat caaattcttc
    23341 atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat cgttcccttg
    23401 aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca gcagaggacg
    23461 attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag aaagatgact
    23521 acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt aatcaactga
    23581 ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta ggaagtctgg
    23641 cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa aagaaaatgg
    23701 tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca ggtcttcctt
    23761 atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt tcgggaagaa
    23821 ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat aatctaagag
    23881 atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt ttatacggag
    23941 gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta aataaaaagt
    24001 atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa aaaataagaa
    24061 aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc aaataagcgc
    24121 ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga ggggttatgt
    24181 gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag gattgacagc
    24241 aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat atttaagtga
    24301 actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt actctttgcc
    24361 aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag tgacttttga
    24421 aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa aagctgcgat
    24481 tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta cagggaagtc
    24541 gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt ctgcagatgc
    24601 gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc tctttggaca
    24661 catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc ccggtctaat
    24721 agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac cgccggaagg
    24781 gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg gagaaagtgc
    24841 acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt cagaaagttt
    24901 cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc ttgcttcacg
    24961 ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag cgaatcgttt
    25021 gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt atgatgcgga
    25081 aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat cttttttaca
    25141 ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt cccttcaagt
    25201 acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat tcatggacag
    25261 caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt cacaagatcc
    25321 agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc tttccgaaac
    25381 cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg cttatttcca
    25441 gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc cagatgaaat
    25501 ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc gtatttataa
    25561 tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc gtgtgagaga
    25621 aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta agagggaatt
    25681 ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg agattccttt
    25741 tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa gtgagcctgt
    25801 taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg cttcaagcat
    25861 gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta atatgccact
    25921 ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa actatcaaga
    25981 gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca ccttcagtga
    26041 tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa gtaccatgat
    26101 tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata tttatcaaaa
    26161 tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa agtctgaaac
    26221 tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag tagctgccaa
    26281 attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga tccagatgca
    26341 gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag aatatgagat
    26401 caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt tttcagccta
    26461 tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg atgagatccc
    26521 gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt ctattgatga
    26581 actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt cgattatcgt
    26641 ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag acggattgat
    26701 caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa tttatcgttt
    26761 ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc aggtaaaaat
    26821 tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa taaaaaaatg
    26881 aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata aaaaagagct
    26941 aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct tgcgttatat
    27001 aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc atgttagttt
    27061 gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca gcagaagaaa
    27121 aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat aatttagaat
    27181 ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa gctcaatttg
    27241 aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa gactatggca
    27301 tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa atcaagtatg
    27361 cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca tggggttaat
    27421 catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg ctaaaatcgg
    27481 tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta tacaagcgca
    27541 tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc aagtgacatt
    27601 attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg atttagcaaa
    27661 agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc ttttttgaac
    27721 ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac tctctttata
    27781 taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat ggagaaatac
    27841 atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt gcgtgatgct
    27901 tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat caatgcaatt
    27961 attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc agcagacaaa
    28021 ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg gaacggtaca
    28081 ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc ccgagcttat
    28141 ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca aggtatttcc
    28201 tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt cgcagatgca
    28261 atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt tggattatgg
    28321 ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat gatcatggga
    28381 gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat caaaatgcct
    28441 gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc gacgatcgct
    28501 ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg gcaactgatc
    28561 attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca agggttggga
    28621 gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat tcatggacca
    28681 aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat caatattgat
    28741 attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga tgcaatcgaa
    28801 gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg gtttggcgga
    28861 tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg tgcagattat
    28921 ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga accagtgatg
    28981 tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat tgctccagta
    29041 gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc ggtatcgcag
    29101 caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg agcggattgg
    29161 cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg ggcaccattt
    29221 gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga tgaacgtgag
    29281 gagcgggacg ctttaagttt cgttcctcat aatgaatttt ctaggaggaa ctgaaatgat
    29341 tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag gatacaatga
    29401 agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct ttgaaacaag
    29461 tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag cacagccttg
    29521 gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa aagataggat
    29581 ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga tcgtgttgta
    29641 tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag gtctttttct
    29701 aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag caaaggagaa
    29761 aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt ttacgataaa
    29821 gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt aactagaaaa
    29881 cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc tcgcataaaa
    29941 atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac gaagaatgaa
    30001 ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt tcattacaca
    30061 gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt aggaacaatc
    30121 ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga agcaacgtat
    30181 taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct agaaggaatt
    30241 tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta ggaaggggca
    30301 gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa atgtttgatt
    30361 tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt tttcgtacac
    30421 ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta acaagtcaag
    30481 tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg gccggcattg
    30541 gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag atcatggaaa
    30601 agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg ccattctcct
    30661 atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt tatcaactaa
    30721 acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt atggattttg
    30781 aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac caacgagctg
    30841 gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc aattaccagt
    30901 ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat cagcttgcaa
    30961 cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt tgcagccttg
    31021 ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca tgcggctacg
    31081 gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg atcacacctt
    31141 atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca tttaaaaaga
    31201 gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg aatgaaggaa
    31261 tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa caaacaaaac
    31321 tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg tatcgtaaag
    31381 tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa gaaacgttaa
    31441 gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa aaaacgggtg
    31501 atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag gaaaatgtta
    31561 aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt tagggttttc
    31621 ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt tagatgaatc
    31681 taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg acctccctac
    31741 attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac gttttgccta
    31801 acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac aagctacaat
    31861 tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc ctaagcgata
    31921 ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct ttgcggtaca
    31981 ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt ttatataaac
    32041 tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga acaatatgaa
    32101 gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat acgctaagta
    32161 tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca tgcaaagtaa
    32221 attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg acggcgtttg
    32281 atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt ttgatttcaa
    32341 aattctcgaa taattctttt tgattgattt taaacttttt taagagatca atcgtatgtt
    32401 tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat tcatttaatt
    32461 tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata tcttcccatg
    32521 ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac acgagcatag
    32581 gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat ttaaattgat
    32641 cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg attactgctt
    32701 ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac gacgctctaa
    32761 tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac ttatttaaaa
    32821 agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata tatttcttta
    32881 tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt ccttctttat
    32941 aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga tcccctttaa
    33001 ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct ccttttgtct
    33061 taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat gaaatttcgt
    33121 attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct ctctttctct
    33181 gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt tgcacatcct
    33241 ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat tggatgtgta
    33301 ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa gaataactga
    33361 acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta ttgttgcatt
    33421 gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga gttaataaat
    33481 tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc acctaatact
    33541 atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa gctaccatct
    33601 attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc gtttccacga
    33661 ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag tacaagaaga
    33721 taatactcct cgttatccca tgaagcgctg ttaattcgaa gtaaacaatt cttataaaga
    33781 tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc atttatccct
    33841 tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc tggaatttga
    33901 tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat gcgtttgaaa
    33961 cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat ataaggaatt
    34021 gctccaatac cgccttcaac tattaattca agatattctt ttttggttag tttttcattg
    34081 ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt gactcctgat
    34141 ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg gggcgtggtt
    34201 catttatttt ttacttactt tactattaat catatcccat tgagcatagg atttttctat
    34261 gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct ttgtaaaggt
    34321 atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg ttgcagaaat
    34381 atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc tagtagtcat
    34441 tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt tagtaaaagc
    34501 tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag caccccagtc
    34561 atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc tgctgttaga
    34621 tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat ttgcctcgtt
    34681 tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa ggagaatcat
    34741 aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta aacctataat
    34801 aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct ctttctcttt
    34861 tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt cttcttgatt
    34921 aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac ttgaaatttg
    34981 tgtttctttg gaattattat ctaatataga ttgttgcttc tctatatatt tttttaaatt
    35041 ttccatttca ttccaatatt tttttgtaaa cataacggtg ttttcatctt tcgtggccgc
    35101 taatatacct ccgcgacttt cgtttccgcc tagcaaggta aattgtatat aaccattact
    35161 catacctggt ttttttaatt gtacggctga tatattttta aaggggatag ttttttctcc
    35221 tttaatacct tgattaacta aatttataaa tccttttcta ataattgaaa tagaatcatc
    35281 agttacttta atcaaggttt ttcctggaga ttttattaaa atctcttttt ccattttttc
    35341 ttcctcattt ctgtgatatg attttttttg tagaagctca tatatgagga aagagcccgt
    35401 gttgcagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata attcttgatg
    35461 tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt ggcagacagc
    35521 ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt tttcgagatt
    35581 aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa ttagaatagc
    35641 tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat cgtacaaaac
    35701 tgaaaattca aaatgagagg cgctgaaatg tgcaagttca tgagataaat gaaaggcttc
    35761 tgcagtttca ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa
    35821 accaggctct ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt
    35881 caatttcaaa tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca
    35941 attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca
    36001 gtatcgtctt ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg
    36061 tagtctacag agacatcgaa atagtcagca atccgactta attcatctga attaggagta
    36121 ttatttttcc actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt
    36181 atagatatct ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa
    36241 acaacctttc taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta
    36301 attctgagta atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca
    36361 aaaaacacta ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta
    36421 tgtcttattt aattatgctc ttagtataga gtattactca gatactgtca attgaattta
    36481 gaaaaaagtt gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca
    36541 ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca
    36601 tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg
    36661 tcgcggcatg tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca
    36721 agagagatga caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt
    36781 ccctctgcca aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa
    36841 catggaggtt atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc
    36901 attcaattag caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag
    36961 attgcagagt acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca
    37021 gtaacaatta gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta
    37081 cttcataaac taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac
    37141 atgaaccaag gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact
    37201 aaaattgtaa tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta
    37261 aaaaaagaag gatattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta
    37321 gtatgactga cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg
    37381 aaagttcaaa gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct
    37441 acttatcttt aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt
    37501 cgattatcac tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat ctagaagcaa
    37561 aaatcccaat tccagaaggc tatgtaatta tctcccaagt ggattatgag gagttaaaaa
    37621 aagctgatga tactggtaga tggatgacgt tgccagaagt gctagaacgg attaacagaa
    37681 aatatgattg gtttacttct agagttttaa agaacccaag atatagaaat attatcgata
    37741 tcgagaaaaa caaaaatgga tttgtctatt atccagttga aggaagagac acatatctat
    37801 ttttaagaag taaaacactt gaatttttag aaacaaattt ttcggaaatc ttaaggaggc
    37861 aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg caccgctaat catctacgct
    37921 ttaggacttt ggggaagcag gcaagcgttg ataggaacga ttgtttacat ggtttggatt
    37981 tttatggggc ttgatgaagc tgaagctgag tacagagcga aaaagccaac cgagggggct
    38041 gactaaaaat gaatagaaaa gagaaactag aatgtatatt actattactc agtttaattc
    38101 tttcactaat ttctctattg ggaagtttgt atttttgata tcaacaattt tgtggatttg
    38161 ggttctattt agagtaaata tcatttttgt ttggtgatta aggagtttat taaaattaat
    38221 tttccctact tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa tatttagcgg
    38281 tacagcttca gagtatattc tattatcatc gactgatcct cctttagcaa ttaatttctt
    38341 aaatggagta gctgaccatc tgcggtttaa gttagcggat gaaagttcta aattaactaa
    38401 catagctggt tctgttgaat agttagaaat gattactttt gtataaatca tatctgcagc
    38461 gaagtaagaa gcattaagtt caacttctag ttgagggcgc attcctttta tttttatccc
    38521 tgttaaaaac gtactcaata taaaacttat tattgacatc cattgaaaaa ttgttaagtg
    38581 aaaaaaattc aaaataaaca ccaccagttt ttaactaaat tataccaaaa aggagagaag
    38641 aaataatgca agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag
    38701 tggcagaaag cttcgagaaa aaacatcaac atgttttaag agatattgat gcactaaaaa
    38761 aagatgtgtc caattttgga cagatgtttg tagaaggtaa tgaaccagat tcatatggca
    38821 gaaatcgacg agttttcttc attagtagag atggattttt cttgctggct atggggttta
    38881 caggaaagaa agctatctac ttcaaacaaa aatacattga agcattcaac gaaatggaag
    38941 atgttattcg caagaatact gttcctcaaa caattgaaga tatgatgatc tatcaattag
    39001 aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg
    39061 gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggga gttctagggg
    39121 gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg
    39181 aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg
    39241 atgctgtttc atttattgaa atgtggttgc cagaaactgc gatccgtatg gaaattgatc
    39301 aactgaacag acaacagaga ctttttggtg atgacaatga atagagctga agcgctaaga
    39361 atagggacgg caattgctaa ttgctggtgg aaatactata aaccaatcat cctaagccaa
    39421 caacatattg acaagcaaaa agcatggcaa caaataaaaa agtgactccg ccggcaagca
    39481 aagagtcaca aagaaaacac atcataagga gattttagca tatggaaaaa gaactttcca
    39541 ctctagatca atatttgatt gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg
    39601 gtcgaaaaat cagacgaaat cttttgacga atgaagaact agcttgggat caagatgatt
    39661 taggcaacca tgtaactatt tgggatcatg tttatcttat ccatctatcg aagcattcga
    39721 ataaacctga atatatttac gtcatcgaag atggcttgat tgatgcgcta gaagagtacg
    39781 acagagataa cttgattgat atctcttatt acggaccagg taagaaatac attgctgaaa
    39841 tggaggcaga atttgatgag tgaaatcaaa gggacaacga actttgaaaa actttttagt
    39901 cgtaagttaa ataaaattct caagaaaaaa ggaaattttg attatttatc ttgggctcac
    39961 gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg taactattaa tgagtacaaa
    40021 cactacaggg ttgtttctgg aactcatcaa gactttcttg ttgaggaata taaacctttt
    40081 cttatggatg aaactgggac ttatgtatct gtctcagtaa cggttaaagg acacacggaa
    40141 accgaattat ttcctgtttt agattatcga aaccaaccag ttgttaaacc aaatgcaatg
    40201 caaatcaata actcattgaa gcgatgcttt gtgaaagcat tggctctaca cggactggga
    40261 ttatatgtat ttcaagggga agatattcca acaccaccta gaatcgatac aaagaaatta
    40321 aacatgctag agacgattct agaagctttc aatgagcaga tgggtaaaga tatgaccaaa
    40381 accttaatcg aatatgttaa tgagcagaca gataaattag ggctcttagc tgataacgtt
    40441 gaaactattg aacagttaag ctatgagcaa tgtgccttga tggagcgagc aatagcagct
    40501 aagagaaaag aattagataa gaagtgatat gagtgtttaa accattaatc gattcatatt
    40561 cagcggttct gaaaaagttc aaaggaaaag acataagcgc aaccatcaat gaggaagtga
    40621 acattgatcg actaaagacg atgtatgacg gctacgatgg tgatcgagtc attgaaattc
    40681 gttttattga tcctagacgt ttcaccgtac agcaacgaaa cttcatctat gcgctgatag
    40741 gcgatatttt tatcgataca ggcatgccaa cggacttctg gaaggaattc ttctactttc
    40801 gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga cgaatcgaat acaactgtga
    40861 gtgatgccaa tgtcttagca aatatcatct tagatttcat ctttgaacat catattcctt
    40921 tcaaagaagg ctatgagatt ttacctgcga atcaagaata ttacttctac aaatgcatca
    40981 caaaaagagt ctgctgcatc tgtggcaaaa caggagctga catcgatcac tttgacaaag
    41041 cgctaggaag acgaaagcgc aaagaagttg atcatgcaga gtacacattt gcagcactct
    41101 gcagaatcca tcacacagag aagcacaaaa taggtgtgat taatttcaaa aataaatatc
    41161 aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt ggagggtaaa
    41221 aatgacagaa catcgaagtt attatgcgat tataccagcc aacgtaaggt acgacaaaag
    41281 acttaaacca aatactaagt tgttatacgg agagataacg gccttgtgta atgaaagagg
    41341 cttttgttgg gcaggcaatg agtactttgc agatttatat ggtgtgaata aagagaccat
    41401 atcgcgatgg gtaagtgatt tgattaagtt tggatacttg aatcgggaaa tcatttacaa
    41461 agagggtacc aatcaaataa tcaataggta cctacgaatt aatcaatacc ctattgacga
    41521 aaaacgcaat acccctattg acgaaaaagt caaagataat aatacatcta ttaataatac
    41581 atttaataat acaaaagaat atataagaga gttaccgcct tcgaaaaaat cgaaggctaa
    41641 gcccgtccgt cataaatacg gagagtataa aaatgttctt ttgtcagatg agcagatgga
    41701 gaaactcaaa acagaattcc ctaatgatta ccaagagcga atcgaacgac tgtcagagta
    41761 ttgtgaatca tctggtaaga cttataaaaa ctatttggca actattcgaa gttgggcaag
    41821 gaaagaaaaa agtgaaccta agaacgcaag cagtggatac aagcgcacag gaagacgaga
    41881 gaagcttcct gaatgggcaa tcgaccaaga agcctatctc aagaaaaaag cgctagaacg
    41941 agctaataga caatcaaaag caccattcta agaggtggaa aaatgaagat cgattatcta
    42001 gaactaatta atgaaatagc gaattataaa aagggcgagg aattagacgt cctgagagac
    42061 gtatatgatc aactcgaaga agctggaatt gaaggaatta agaatgatcg ttcgagttgg
    42121 agtaaactca gatactattt cgcactctat atcgatggaa cgcaattaag aaatttagca
    42181 tataccaaat tactattcat tgattgtgtt aaaggcttgc aaaaacatct taatgaactt
    42241 gagcaggtgt aaacaagatg gacctaaaga catttacagc acagatcgaa ttaatgcatc
    42301 aagaagcttt aagacaaagt gtgtcgtacg aagacaagtg gctcaacaca tttcatggcg
    42361 gacgtgagag cgcacttgat caagtactca aattactgaa aggagaatgt cgggatggat
    42421 aagaaagcgg caatgcagcg aattatcgaa ttgacttatt cagaagattg gcaggaagat
    42481 aaaaaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga aaagcccaaa
    42541 cggagaacgc cgagaaagat tgcaatctgg catggtgatc gaattctagt aacgggtacc
    42601 gctaaacagt tatctgaaat tactggtctg agcaaaaata ttatttggga tagagctagg
    42661 agcttatgga ttgattcaaa aggaagacag tttaggtatg tggaggagaa ataatggacg
    42721 aactaatcgc gaaagtagag cagtgggcaa aagataaggg attggatcaa gcggatttca
    42781 gcaagcaaat gttaaaaacg gtcgaagaaa tcggggaagt tgccgcttct ctagctagaa
    42841 aagatgaaca tggtttaaga gacggaattg gcgatgtagt agtaaccttg atcatcttgg
    42901 caatgcaaaa tgatatggat ttatacgagt gcttggattt tgcttatgac gaaatcaaag
    42961 gtcggactgg aaagatggtc aatggtgttt tcgttaagtc cagtgacctg aaataattcc
    43021 attaatggaa gaaataactg aataggagga tagacatgat tgatgcaaaa caagtgcaaa
    43081 aacagaaaga tggaatgttg atgtttgaag catatgttct accatttctt aatcaatttg
    43141 aagttttgga atgctctgcc agcggagaag aattggagta tgttgtaatt cgtgaaacca
    43201 aagaaaacgt tcagaagcta aacgaatttc tttgcactat taactgttgg gatatgatag
    43261 ctccaggatt tttatgtcca gctatgggag aatttttgga atattgtcgt cttgaagatg
    43321 caggcacttt ggatttagct tatctggtat acaactacct aaacatcaac acagatcatt
    43381 tgtggtttgg cactgccgaa agaaaatggg tagtccgcta accaccaata tcaatgaaaa
    43441 aaggcggttt cctgaaagtg gaattgccgc cattgctgta aggttcttta cgcgactcaa
    43501 ggtcacagaa atataaatgc aaatctcttt tgaagtgatc caaagctctc ggaaaatgga
    43561 agttttttgt ttcaaagaat aaatgagatt tgcgtcaaga acctgagagt tatttccaaa
    43621 aaagcttcca aagaaccgaa tgagctaagg acatcatgtg taaacacata atatagctct
    43681 cctttcacgg ctaatcataa catgaaaaat attcgatgtc agtaggaaaa aaggtgaatt
    43741 atgaaaagaa tattaaatta tcccggcagt aacagattct gcaattaaac tccgaagctg
    43801 atgtatcagt atcaactgta gaatattatg agcgtggaga aagtcaagca gtcacaaatg
    43861 taaaaatcga taacgtcaac ggtcttatct gtttagatga aggcgatagt atgaatgaaa
    43921 ttgctgcaga ccatgaatta tcagaaactg ctttaattga tcaaatcgaa ttagctagac
    43981 aatactttgt tccatatgca gatacctggt ccaagcataa gaatgacatc atttttccag
    44041 aggaacaatg agagcaactg atccagtaat tatccttgag gaagccaaat ttatttggac
    44101 tcacgaagag atagagcaag cacgcttgct cttttctcaa ggagttaagc cgagcaaagt
    44161 agctgaaata atgggtcaaa agattcttga tgtcggattg cttttgctcc atctagcaga
    44221 aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa acagggaaaa
    44281 tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa acttacacga
    44341 acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat cagattatgc
    44401 cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc agccgtgtgg
    44461 gctggagttg ctatttcttt ttaataatga gtttttctaa atttaattca ttctcacgag
    44521 tttgcgtcca ataatcagaa taatatttta attgtttaag agtaggaatg tcgtcttcgg
    44581 taaaattaga aaataattct ggagaattat ctttttttaa ttgttctaat cctgattctt
    44641 taaaacgttg ttcaacgagt tttactaaat tgttgatatt catgataaat caccttttta
    44701 aatttaattt cgatttgatt aatcgataca agtattatat caaataaaaa agctgattta
    44761 gggggtctta caaaaacact aaaatttttt caaaaagagg aggaacggtg gtgaaagtca
    44821 gagaacaatt caagatagtg agcgacgaag ataatgttta caaaataaag atcgttaata
    44881 ttaatgaata tcgagaagca gatcacaaat atttggttga cttatacatg aacgataaat
    44941 atttgggaga aggattttgt ggagattcac tttttgatga tgagcgaatt ttgaaggagg
    45001 aaacagaatg atccacgaac taaaaatact accagaatat tttgaagcag tgacgagcgg
    45061 acgcaaacaa ttcgagatcc gcaagaatga tcgtgacttc aaagttggtg atcagttaat
    45121 actacgtgaa tataaaaaat acattaaaca tgaattacct ggactaacga gaccaacatt
    45181 tagttacact ggtgactcat acaaagcaga gatcacttat attactgatt acatgcagaa
    45241 gtccggatat gtagtgttgg ggattaaagg ggtgaattaa atgaaagtat acatcttagc
    45301 tatcacagag ggcacatgga tgttcccagt cggttccgga aaaatctata aatcaaaaac
    45361 agctgcttat aaagcatttg aaaaatacaa aaaagaaaac ggtggcggca ctaacgcaaa
    45421 gattctagtg gctgataact ggcatgaaga aggtgaacgt aattgattta cctgttcatt
    45481 ttcgtggtac taactatttt tatgctagta atcttattca aaaaaagatg agtaaagacg
    45541 aacgaaaaat tctggccaac ttttatttag tattaaaaat cgaacaaaat gtgttttcgg
    45601 aaaggagaat tgtatgggaa aaacaaaatc aaagattaag aagaaaaaac gacgtttgga
    45661 acaaaaggca atccagaatg gaacggctaa gaaaaaataa aaaagctacc cctttcgaga
    45721 tagcagcgca atactattct accataaggg gtggcgtttg tgagatttca ttggttaaaa
    45781 gattaccaag aactagatga gcagattctt tacttaaagt ggaatcttaa taaaagtaag
    45841 cttgaattga atcgatgggt ctgtggtgat ttagcaaacg tccgcatcga aaagaattcg
    45901 agaacttctt ctttagaaga gaatatccaa aagatagaaa atgaattgga attgttgatc
    45961 gagcaaaaag aagaaatgtt attgctgata gatagctttt ctggtatcga taatcaaatt
    46021 gttaagatga aatatgttga tcaaatgagc ttagaagata ttgctgaatc tgttgggtat
    46081 agctcatcgt atgtcagaca gcgacatgca gaaattagaa agacattgaa ctttttagat
    46141 gagtatgaac acagacaagc tgaccgattg aaaaaagaaa acgaaattga tttttacaat
    46201 agcgagaagt acaaagaaca actctcttta ttctaaaatt acactgttca ctaaatgttc
    46261 ggtcattgta gctatgtaaa cattgttagc agcatgatat tctattagtg tcaaaaaaat
    46321 atgaaagagc caagatatcc caaccgtttt attaattggt atctgtggct cttttattct
    46381 tttaattagt agttggcata aagttatttc catttataaa ttgatcagct aaatcagcat
    46441 cataatcagc cgcgataaat tgcgctgctg atttaataaa tgcatccata tcatgaacat
    46501 ctttattagt atgtctacga acatagtgcg tttcatcatt accaatccaa gtagcagctt
    46561 tggctaatct ttggagttta ggaaaatcat tcaaataatt ttcaattgtt ttacctaata
    46621 acattttttt tattttatct tcatcctctg aatttttaga tattgcataa tctttaatta
    46681 gaaattctag agcttttcta taaccaactc cagcaatttg ttccaattgc tcagatttag
    46741 cttgtgtggc ttgagtataa atatcaacaa atgtaggaga aactttttca atattttctg
    46801 gtaaatctat tttaatcggc ggacggtaat aatctttaat taaaatattt tcagaatatc
    46861 cttcatcttc attaaacgta tttataaatt gtaaactata aaatttgttg cactcatcat
    46921 ttatacactg tactaatacg ccaaagattc tatcattatc gaacgcacta tatgatgaaa
    46981 ccccaaaata cacatacgga gtcattatat ttccgcaatg cggacaacta tttggcaaat
    47041 aaattgtctc tgtattttga cgatcacgat acttaatatt aacttcttga agcattaaaa
    47101 cacctcgcat cttttttata aatcatacca caaaatatct gataggagag taaacaaatg
    47161 aatgattttc atgaagcggt acttagtatt gaagtagaat caagtttggc taaagtttat
    47221 aagaaagcaa ttgaagcaga gaatagtccg tacagagaaa attggaatgg taatcatgca
    47281 catgtgcaag tggagaatga tgattacaga actggaatga atacgttagt tatttcttta
    47341 ttgtcacaca ctctacccaa tctgcaggaa acgattgaat ggtatgagcg aatgggcgca
    47401 aaagtaatcc gtacaaatta caaaggagag aattaaaatg gcaatgatca aaattagaac
    47461 atccattagt ggaacagagt attgggattc agaaaagaaa aggacagtag ttgttccgaa
    47521 agatcaagaa ccaaattttg aagtaaaaga agaaaaagga attttgagtg atggcaaatc
    47581 atttgttgct gtcaatggcg aactcatagc tcatagcagt gaaattcttg atagcgatgg
    47641 aaacactgct gctaattttg atggagacga agctactaat gatcagtcca ctgaagatac
    47701 ggatgaactg gacaatatga ctgcaaaaga attgcgtgca tatgctaaga aacatggtat
    47761 tgatattcct ggtgctatcc gtgcaaaagg cgatatcata aatcttattc gtgaagtaga
    47821 atgaaatact gtcagtttga cggatgcacg aacaaaatag caaagggtat ctactgtact
    47881 gaacacaaga gatcaagcag atcacgcaag aagaagcagc aagcaaagtc tgtttatcat
    47941 catgagaaca aaccattcta tcgaacgcaa gcatggaaag atatgcgtca atttatttat
    48001 gaaagagaag gtggtcattg tcagcggtgt ggtcagttca tatttggaaa gagagcacac
    48061 gtccatcaca ttgtaccaat caaagacaac gaactgctta agcttgatcc aaacaatctc
    48121 atgctattat gttcaaaatg tcatccaatt gttgaaaacg aaacggaaga caaaaaagtt
    48181 tttccttcgt atttcaattg aagcccccct attcattttc aaaatttttt cgcgtgggga
    48241 gatagggtag cggggagtca cgcgcatcgt taggtcaaat ttttcaaaaa acaaaggggg
    48301 gtgtatacaa aaatgacgac taaagcgcaa cgtaaagcga ttattgatga aaaagtaagt
    48361 gctgaaaaag ctcgtatctt agaaataatg aatttgtctg atttgtacac catcactctt
    48421 gatccattaa tcgaatcata cttggatatt tttgaaattt accaacacaa atatttattg
    48481 tggaaggaaa aaggatttcc ggagacgcaa aaattcacga ataaatcagg agctactaat
    48541 caatcaaaac atccattggc acaacaagta gaaacttggg cagataagaa gatgaaagct
    48601 ctagatttat taggattaac aaacaaagca aaaactggta gactaattgc tggaggatcg
    48661 accgcaagaa aagatgaaga aattacacgc ccagaagtaa agccagtaga tgaactagca
    48721 gcgcacagaa ataaatggcg taagaaggca ggtgctgaaa aatgattgaa cctggtgtaa
    48781 attatgctga tttatttgca aaagaagtaa gaaagaaacc taagaagtat cctaagaccg
    48841 ttcgtttagc aatagatcgc tggtatcagt ggaaaaaaag aaaagatatt tggtttgatg
    48901 tagatcgcgc gaatgaaatg atggattggg tagaatcatt tattgttcac acaaaaggag
    48961 aaatggtggg aaaaccgttt cttttagaac cctgggaaaa atttatttat tcttggattt
    49021 atggttgggt taaagaaaat gaaaaaggcc aaattgtacg cgttactcgt gaggcttatg
    49081 tccagattcc aaaaaagaat ggtaaaacat tgattgctgt cggttcatta ggatatgcaa
    49141 tgtacggaga aggggcgtta tcagttgatt gctatgcttg cgcatctgat tttgctcaag
    49201 cgcaatatgc tgccaaacct tttgcagtta caatcttaaa caatccaatc ctacttgaag
    49261 gaactaaaat atttaggggg ccaaagggaa ctgtttctag tattacttat gactatattc
    49321 atgaagatat ggcttactca aataaattta ttgttcaaac caagaatatt gataatatcg
    49381 agggatccaa tccatatttt gtattgaacg atgaattgca taaacaggag aaaatggaac
    49441 agtatgacaa ttttaagtct gctcagatat ctttaccaca gccattgatg tttaatatct
    49501 caactgctgg gaaaggttcg tcgtctgttg gaatgcgtgt atatcgcgaa gcaaaagaag
    49561 tgttgaaacg tgatgataat gattcaaact ttgttctaat ctatgagcca aataaaaatt
    49621 atgattggac ggatagaaaa gtctgggaaa tgtgcaatcc taactgggga atatcagtcg
    49681 atttatctgc tttagaatcg gcatttaaaa cagcgcaacg gtcagctcat tccaaagctg
    49741 aatttctaac gaaacacttg gatgtatttg tgaacggcgc ggataatttc tttgaacagg
    49801 atcaagtaga gccatgttta gttaccacac aagaacttgg tgatttaagt ggcgaaccat
    49861 gttatatcgg tttagattta tcacgtacac gagatttgac ctgtgtatct ttaaacttcc
    49921 caacatggga tgaagatggt aaagcggttc ttaaagtaaa gcaactttat tttattccga
    49981 atgaagattt agagtttcga gaaaaagaag ataatgttcc ttacagtgat ttggctgaac
    50041 aaggtttcgt tgaattttgc gacggaaaaa tgattgatca agatcagatt ttgcagtaca
    50101 tagaggactg catgaattta tatgatattc agcaagtgaa ttatgatcca gcgatgagtg
    50161 acaaacttgt tgagaaatta gaaaacttag gattggaatg cgttgaagtt gctcaatatc
    50221 ctaaagtatt gaatgctcct tttgacgatg tcgaacggtt gttttatgag aaacgaattc
    50281 aatttgataa tccattattc ctttattgca ctttgaacgt tgtagcaatt actaacatca
    50341 atggacaaaa agcgccaagt aaacgtcaat caaagaaaaa gattgatggg ttcgtggcgt
    50401 ttttgtgtgg tcataaggaa acgatgaatc aaatgacaga tattgattca gatgagttag
    50461 atgattacct aagttccatc tatagataaa tagaaaggcg gtgaaaaaaa ttgaaattac
    50521 gtgatcggtt atcgaatgca gtctattctt ttatggaaaa acgtggatat atcgaggata
    50581 tttttggaca ctacacacgt tatggtcaaa gatatgtgac ggattcatct atcatggaat
    50641 cttctgatgt ttatgaatta gttcaagaca tatctaatca ggtggcattg gccacaccaa
    50701 ttgttattgg tcccgacggc aacgaagtca aagatcatca cttactaaat attttgaaga
    50761 gtcccaatga ttatttgaca ggattcgaat ttaccaaatt ggaaacaaat actttattga
    50821 tcaatggtga gacattccca ttaacggata gggatcagct tcatttggcg tacggtgtaa
    50881 caactaaaat caatgaacga cttcaagaag aatttgaaat gaatggtcaa aaaatacctg
    50941 gtcaaatgat tcgacacatt aagaacatcg gaactgattc attaaaaggt gctggaataa
    51001 ttgatcttgc aagaaacact ctagaaggcg ttctgagcgc tgaaaaagtt ttgacggaca
    51061 aatatactaa aggcggttta cttgcgttca tgcttaaact agacgcccac atcaatccaa
    51121 ataatagcgc ccaaacaaaa attgtcaaag ctatattgga tcaactggaa ggaacgcaaa
    51181 atgagagtga tcattctgtt aagatgattc ctttgggaaa aggatattcc atcgagacat
    51241 taaaaagtcc tgttgatgac gcggcaattt tgaattattt aggtgtttac aaaaaagact
    51301 taggaaaatt tctaggaatc aatgttgata cgtatcaatc gctgatgaag acagatattg
    51361 aaaaagcgat gatgtatctg cacaacaaag caatcaaacc aatattgaaa aacaagagcg
    51421 aacattacac cgctcttttt tttatgccta attctggcta tagagtggaa tggaaaatta
    51481 atattttgga ttttgttcct tactcaacaa aaacaaatat tggctacaac atcgttcgta
    51541 ccgggattac aagtccagat aacgtggcag aaatgcttgg ttttcctaaa cagaatactc
    51601 cagaaacaca agctatctat atttcaaatg acttatctag gattggccag aaaaatgcaa
    51661 cagatgattc cttaccaacg aatgatcaag acttgaaagg aggtgatgga aatgaagaag
    51721 aaggaaattc gcacgattga catcaccaat ctttcaacac gttccgatga agaaactcat
    51781 acgaggacca ttagcggcta cgctgctgta ttcaacagtc aaacactttt atgggatgac
    51841 ttaagcgaag tgattgttcc tggtgcattc tcaaaggcaa ttagtaattc ggatgttcgt
    51901 tgtttgttca atcacgattg gtccaatgtg ctaggacgta ctaaaagcgg aacccttcgc
    51961 cttgaagaag atgaacgtgg tttgaaattc gaagttgatt taccggacac aacggtagca
    52021 agggacttag ttaaatctat ggaacgagga gacatcaacc aatgtagttt tggttttgtg
    52081 ccgactgaag aaacgtggga ctacaattct gaaccaatgc ttcgaacaat ccatgaagtg
    52141 gaattatacg aggtttctat tgtgcctttg ccagcatacg aagatacaga agctgcatta
    52201 agaagtcgtg atgaattaga aaaaaacgtc gaagaaagaa aaaaattaat caaaaaaatt
    52261 aatcaagcgc tagaagcgta ggaggaaaaa tacatggata aagaattatt gaaaaaaatg
    52321 aaggcacgtc gtgagcaacg attgactgaa ttacgtgaaa aagttgaatc aggagaatta
    52381 cgcgaagcag atttagaagc cgtgaaagaa gaaattgaca gtgttattga tgaattgaac
    52441 ggaattaaag acgaattagg cgcagattct ggaactgatg aaacagacga caatacagat
    52501 gatcaatcga atagtacgga ttcagacgaa agccgttctg gtgaagacaa cgatcaagaa
    52561 gaagattcag acagtgaaga tagttcagaa aatcgttctg gaatgattac tcaacagcaa
    52621 cgagatggat tacttggatc aattaagaac ggattggagg cacgtgcaaa aatgaccaag
    52681 aaacaaaaag atcaacaact acgaaaagca tttgctaatt ttgtagctgg aaatatttct
    52741 gaagcagaag ctcgagcttt agggattgaa gctggcaacg gttcagttac tgtcccagaa
    52801 gtaattgcat ctgaagttat tacttatgct caagaagaaa atttacttcg taaatacgga
    52861 acagtggtgc gaacatcagg agatgtcaaa tatccaattc ttgtgaagaa agcagatgcg
    52921 aatgtaaaca agaaagagcg ttcaactgat attgctgaaa cagctattca gtttgatgaa
    52981 attttgctta atcctgccga attcgatgct ttggcaacag taactaaaaa attattaaaa
    53041 atgtctggtg ttccagttga agatattgtt gtggaagaat tgaaaaaagc ttatgtgcgt
    53101 aaagaaatca attatatgtt caatggtgat gacgctggaa atgaaaatcc tggtgcatta
    53161 gccaaaaagg ctgtagcatt taaaaaacct ttagatctaa ctgcttcagg tgctgggcaa
    53221 aaattatatg atgcattaat cgaatttaaa aatacaccag tgacagaagt gatgaaaaag
    53281 ggacgcttta ttattaatcg agctgctttg actgccattg aaaaaatgaa aacagatgat
    53341 ggatttcctt tgttgcggcc attcacacaa gcagaaggtg gaattggtta ccaattagtt
    53401 ggctatcctg tggattggac agatgcagca gataaaaagg gtgaaccaga cacgccagtt
    53461 ttatattttg gcgatttttc tgcatttaaa attcaagaag ttattggtgc cttggaaatt
    53521 caaaaacttg ttgaaaaatt ctctggtaaa aatcaaattg gattccaaat ttacaacttg
    53581 ctagatggtc aattggttta ttctccattt gaaccggcag tatatcgcta cgaaattaca
    53641 aaaccagttg gtggttaaga tggaagagca aactaaagaa ttgtctttag aggaaaaatt
    53701 caaatcacat attcattttg aagagggcat ggatgattct ttgctctctt tttatttaaa
    53761 tatggcaaaa gattatgtca aaacagcaac tggtggccaa caagaatatc ttattttgat
    53821 ggttgccggc attgcctatg aatatagagt ttcagaggat gaactcgaca aagctatgaa
    53881 tgccatgacg ccatttatcg tgcaaggagc gattcaaaat gccgaagaga cagaccaata
    53941 atctcagatg gaaagccgaa ttgctagaca tcaaaacagg aacagacgga aacgatcgtc
    54001 caactacaat ttacgaattt aagcgtctaa tattctatga agaactcggt gtgacttctc
    54061 aagaaaaata tttatcacag caagccaaga cagacgttgt cagacgaatt aaagtcagat
    54121 gggataaatc catcacagag aaattaagtg cgctcaaaat tgattctgta acgtataaca
    54181 ttactcgcat ttatacgaat cccgatgcaa gagaaatgga gttgagttta gcttatgtcg
    54241 attagctttg atgaattgaa aacagcgctg aaatcaacaa agttagcagt gttcagagac
    54301 aaagccagat tagggacgat gtatccatac attgtgtact caaatgtgag taacagcaaa
    54361 aaaatggcat ccggtaaagt ctataaaaaa ttaccttatt accaagtctc tttttttaca
    54421 cttggaacag aacaggattt agttgttatt gaacaagcgc tgcaaaatgc tggcattcca
    54481 tattcagatt ttacaggtat acaaggtgat gagaacgacg ataccgtaac caactattac
    54541 acatatgtga ggtgtgtgga aaatgccaag taataagaat ggtttttctg aaatatcgga
    54601 ttatttagga aacctttcaa aagttgatcc gaaaaaatta tcgttggaat ctttagaaga
    54661 agcagctaac ttttatctaa agcagttgct tcctaatatt cctaagtcgc tcttaaaaaa
    54721 gaaacatatg cgcgatcaaa taaaagtagt agttgaaaac gatcaagtga aagttcaatt
    54781 tgaagagacg gccttttact ggcgctttgc cgaaaatggc acaacaaatc aacgtgccca
    54841 acattttgca agtggtacgt atgaacaaaa caaagaaaaa attgaagaaa ttatgactag
    54901 aaaaatactt gatttatggg aagggtgatt ttaattggga aaacaagata ctttttattt
    54961 tgaaggattg gacgacattt taattgcgat gatggcaacg ccagattctg ttggaactgc
    55021 accgacttat agtgaagttt tacgattgcc aattgccaca aaaatcggtg tcaaagggaa
    55081 tggaacagca ttagaaaaat gggcatccag caaaatgttt cgtcgtgtgt cgcgagaaac
    55141 aaaacatgaa cttgcgttgg atcacgtggg tattccgata gaagtgatgg atgaaatcaa
    55201 aggattagtt gctaaaagcg gagtaacgtt tagcaaaaat accgcacgag aatttccgta
    55261 cttcgccttt ggatttattg gaaatattga aaatggcggt aaaaaagcag tttggtatcc
    55321 taatacacaa ctatccaatg tgattgacga agagtatgcc actgcagagg aagaaacaaa
    55381 aattgacgat gtaactgcaa atctggtttc catcggactg aaatacaata atgtgatgta
    55441 tgcaagtttt gattcaaata gagacggagc ttctctagaa ttatttaata aattcattgc
    55501 tcaacctgta tacgatgaag aacagtggaa aacattggct aaagttggag gtgcgggtta
    55561 atggctcggt tatctgatta tggaattcac gttgaagact taaaaaattc tgctgttgta
    55621 attattcaag gcgcagaatt ccctatttca tttactatgc aaacgatgga atttatagca
    55681 gatgtgtatg gtggagatta ttcgcaattt gaatctgata tgaacgccat gctatctaaa
    55741 aaagaaggac aaatttcttc tgctaactta tcgcctagtg atctaaaaat catgcgtgcc
    55801 ttgatttatg ccatgctgcg tactggtggt ttagaggaag atccagaaac tattttcaaa
    55861 tttttgggaa tgagtggaga ggtgttgtct gcttatagta cctgtatgga aatttttgct
    55921 agccagacat ttcaggtgga agacctaaaa aaatccaaga agccacaaga ctttcaaaaa
    55981 gcgcaagcaa aaagaaagaa aaacaaaaag aatcggaaga gataggaact ccttggagtt
    56041 tttatattta cgttgctctc actctattga attggagtga gagtttcttt ttaaagtcta
    56101 cacctaactt gtggctcaaa tcttatttac agtggttaca acaaaacacc gattttgaac
    56161 cacctcaatc tgtaactatg gataaatcgc cttggtggta gaaaggagcg ctaagataaa
    56221 tggctggtaa agaatctgat gtcgttctta attttaaaac gaatggcgaa gtcagttatt
    56281 cgaaaacaat caaagaaatc aacaaagaaa tgaacttagc cgctgccgag tacaaaaacc
    56341 aagtgtctgc gatggacaag gatgcaactc aaacagaaaa attgcgagcg gctaaacaaa
    56401 aattagaaaa acaattagga ttggctgaac aacgatctca aatgctgaga gaggaatatg
    56461 aaaaatctgt caaagaaacc ggtgaatatt ctgctgaatc agaaaaactt tataaacaat
    56521 tactcaattc tgaaaccgga gaaaataaac ttcgtacagc attagaacag acgaacgatg
    56581 cacttaaaga acaaggtgac gtttctgttg atacagcaaa aaaactccag aaaatcgaag
    56641 aaacaggtga gaaagtaaaa ggcgttggtg aaaaaatgtc tgttggagta accgcgccta
    56701 ttgtagcggc aggagcagca ggacttgcag catttggtga agttgacgag gcacttgata
    56761 ccatcattac aaaaaccgga gcaacaggtg atcaagctga tagactttca cagtctttca
    56821 aaaacgttgg ttcaaatact catttacctt tacaaacggt tggagaagct attggtgagg
    56881 taaatacaca atttggattc atggataaaa aactggaaga ttcaaccaat tatctcctac
    56941 agtacgctga aatcaatgat acagatgttt cgcaatcagc aatatctgct cgacaagcta
    57001 ttgatgctta tggactagaa tatgatgatt tgaattctgt ccttgatgta acaacgaaaa
    57061 catcgcagaa tactggtcaa tctgtagacg acttgatgca aaaagcaatt gatggcgcac
    57121 cacaaattaa acaattaggg ttgagctttg gtgaaggggt cactttactt ggacaatttg
    57181 agcaatctgg tgttgattca agcgcagctt taagtagttt atctaaagca acagtggctt
    57241 atgcgaaaga cggaaaatct ttaagtcaag gactagaaga actacaagac aaggtaaaaa
    57301 atgcaggttc tgaaacagaa gccatcaatg cagcagcaga agtattcggg actaaaggcg
    57361 gtcctagaat ggccgatgca attcgtagag gtactttgaa cctggaagat ttagcaaaaa
    57421 ctgctggaga aagtggggga gctgtaggag acacattcga tgctactctc gacccaattg
    57481 atcaagccga tcaagctatg aacaatgcaa aattagcaat ggctgacgta ggtgaagcag
    57541 tccaagtaag ccttttgcct ttttttgaaa aagcaacttc cttattacag gaattctcta
    57601 aatggtgggg gtctctagat caagatacaa agaattggat cataacaatt gctggtatag
    57661 cggcagcagt tggaccagta ctaatcgttc ttggttcact tatgggatct gttacaaaaa
    57721 tcgttgggga agttaaaagt tttataggag tttggcaagg acttgctggt ttatttggaa
    57781 tgtcgggagg ctggtttgca ttagcagtta tagctattgg cgcattagtt actggactaa
    57841 tttgggcata taataacgtt aaatggtttc atgatggagt gaattctttc ttcaaaggtg
    57901 tttcagatgt agctgttgaa atatttaatt ttttaagtgg ttattttggt ggatttttcg
    57961 atggtgttat agcaaatttc aataattttt ataatgctgg aaaacgaatt ttcagtggtt
    58021 ttctagattt tatcactgga gtatttacag ggaattggtc aaaagcatgg caaggtctag
    58081 ttgatatttt tggaggtatt ttcgatggta ttgttgctgt agggaaagct cctattaatg
    58141 ccatgattgg attgatcaat ggatttattg gtggattgaa taatataaag ataccaaaat
    58201 gggttcctgg tgtaggtgga aaatcatttt ctatttcaaa actgccttat ttagcacaag
    58261 gcggtcatct aatcaatgga caagcgattg ttggtgaagc tggacctgag ttactgactg
    58321 ccaagaatgg taaaaccaca gttactccat tatcagacga agaaaaacgc aaagggatca
    58381 gtggaagagt ttctggaggt agcattgaac agcatataca cattggcaaa gtcgatgcaa
    58441 acaatccatc tgagttagat cgtatgaatc gtaaatttgc taaagcaaac aaacaggcta
    58501 ttcgtgattt gggaggtgtt cctatttgag tcgacaattt atgaatccag atgaaccaaa
    58561 ttttatttgg aaaaatcgga atgcagtaat tgatatgaat tgcatcattg aggcagagct
    58621 tccagatatt atgccgaaca agcgatacga aacttacacg attcagggcc gaagcggaga
    58681 attgacggag acgtttgatg actatgaacc ttttgatttg gaaatagaag ggatcactat
    58741 tccacattcg aaactgagag aagtcaaacg atggctcact ggtaaaagcc aattaatcac
    58801 acataatgat ccagacaagt atctagaggc tatctgcagc atggataaag aagtcccttt
    58861 cgaaaacgag tggggcttct tttatacatt cgatgttact tttcgttgtc agccactcaa
    58921 aagaaaaatc ggtgaagcac ctaaaatttt tcattctagt gttatggata tttttgatca
    58981 tggtgacgaa attgcacatc cctatctgga aatagaatcg aacggaggag atataacgat
    59041 cgctgtcggt gataaaagcc taacaatact taatacgtta gctgcaacaa tcgtggttga
    59101 tactcagctt ggaaaagcaa tacaagaagg tttgaattta tttacgcgtg gtgattggcc
    59161 agtattgcag cctgaatgga atcaggtaaa aatatccgga aaatttaaag aagtgcggtt
    59221 ttggaatagg agtgtgtatc tgtgacgcaa gaatttattt atgcttataa aaaaatgcca
    59281 gatgatttga gcgtgaacgg agcgtcgtta gttgattggg aagatttgcc agaaatcaat
    59341 cgtgtgctga atggccaata tcgtttttat ggtaactatt caagaagcgg acagtatcgc
    59401 tcttatttaa aaaaaggaaa ttttataaaa gcgaaggtac cagatggatc atggcaatat
    59461 tttgaaattt ataatgtcaa aaagaatttg acctccgttt ctgtaacagc tcggcatatt
    59521 ggttttatgg ccaataagaa ctttattgtc aagtcgttta cggataacgg caatgggtct
    59581 cagatcatga ccaatcttaa aaacagtttg gcatttgatc aaaaatttaa ctatctttcg
    59641 aatgtaggga caacgcacca atttacagca agacaagtcg cgccagttga agcaatcatt
    59701 ggttcaaata acggtaatca aaatttgact ggcgtgacta gcgcggaact tgatatggat
    59761 aactatgatt tgaaactggt aaaacagatt ggatcagata atggttttcg gattgatttt
    59821 ggcatcaatt tagaagcaat cgaagaagaa attgatgaag aatcaattgt caatagtttg
    59881 tatttggttg gcggtgttcc tgataacgat tatgatgaag acaaagaacc tatcgaatat
    59941 ggttatttag aaattgatgg agtaaccaac gagaatcgac gtattgccaa acgtgaaaac
    60001 ggagattgta aaacagttga tgaattgatc aaatggggca aaaccttatt tgacaatgat
    60061 cgtatccatg agccaaaagc tactcataca gtcagtatgg tggcgttgga acacacactc
    60121 gaatacggtg acatgtatca agagctagcg tctttacact ttggtgatgt cgctcatgtt
    60181 agggcgaaag agctagatat cgagataaaa gagcgtatgg tcgaatacac ttatttccca
    60241 acgcttggga aatataagga tattgtttta ggtaatgatt tatcactgta cacttcggca
    60301 gtaaatactc aagcacaaga attgaaaaag aaaatcgaca atcgaacaga aacgttagtt
    60361 caaaatgtgt tgaatgcgac ggcatggatc actggtaatt ctggtgggca tgtcgttttt
    60421 cgtccagaaa aagctccatc agagattctt atcatggata ctccaaatgt ggcgaatgca
    60481 aagcgagtgt ggcgttggaa tttaaatggc ttaggttatt cagacaacgg agtaaatggc
    60541 ccgttcggta ttgctatcac atcaaaagga gaaattgttg ctgacttcat caaagtagga
    60601 acaataaatg cagaagtatt cgaaacttcc tttaatgctt atggtgatgt gttaaaactt
    60661 gtaaaaggca cgcttcaaat ttggaatgaa aacaaaaaaa tcatggagct aaccaaaaaa
    60721 ggtatggaat tctggaatag aaaagaatcc attggaacga tcggaacaac agattcagca
    60781 ggaaatccat ttccaaatgc tgtaacacct acacctcttg aggaaaattc tttggtaata
    60841 cgtacaaatg gcgatggaaa atacatttta atttctccaa ctgcagaaaa aggatttgtg
    60901 ttattaggaa acggaaaagc atattatttc ggtgatttag atattcaggg aaaacttact
    60961 gttagaggta aagaagtgat accaggacaa aatggcggtc catctggcgg aggagaaaca
    61021 cctggtgggt atcctgatga attaaaaaca gatgcagaaa agagagcttg gagaatatac
    61081 gatattttgt gtaataacgg atttacgaaa caatctgcat gtggaatatt ggggaacatt
    61141 caacaagaaa caggaggaac ttttgatcct gatactgttc aaataggtgg accagcatac
    61201 ggattagttc agtgggatgg ttcttcatat cctcttgttg gtccagcaac atgggatgga
    61261 aaagtttatg ttcaaaactt atttaatgct gcaggtatta aagaaccaat aacgagctta
    61321 gatgcacaag ttcgattgct tatttggaca tttacaaatg gacaatggat gggcgtagta
    61381 caacctacga cggttgatgg atttaaggcc tgtactgatc caagacaagc agcatatgct
    61441 tttgaacgaa actatgaacg tccggcagcg acacatcctg aacgtcaaga ttatgcagtt
    61501 aactggtata acaaatttaa agatttaaaa ccaggaggag ctactggaga agcaggacta
    61561 aaacatttag agtctttgat tggacaacga attggcaatg gtcagtgtta tggcttgtct
    61621 gcagaatatt caggatatct gggtggttgt ggcatgggtg ctggaacaaa atatggttta
    61681 actcatgtga taggaaatac ttctgcagca tctgatattg gtattgccta tgattggtct
    61741 gctgttggtt ggaaagtgat tcaaaaccct agatatgatc aattagtagt tggtgcaatt
    61801 attaattggg caagaggtgg acaagtgggt tcatggtttg cagacggaac ttatggacat
    61861 actggcgtta tccgaggctt agctaatggt cgtatgcaaa cttatgaaca aaacacagag
    61921 ttaggtatga tttgtggaaa gttagatcgc cagtattata gtgcaagtgc aatttcttcc
    61981 attgtcatac caccaaaata gggaggtgat ttaatggcaa agtggaatgt catattaagt
    62041 acaacagaac cctataatta cgtggggatg attcaagttc gacaaggcaa taaaaacact
    62101 gaagttatgg aagctacaat tgtcgaaaat ggtcttccat atgatttatc agaatgtaag
    62161 gtatattttg aatcagttgt tggcggaaaa tatccagtcc aattagaaac aagaattgtg
    62221 gatgctaaaa aagggaaaat caa
//
LOCUS       DACEND010000002        56006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEND010000002 DACEND010000000
VERSION     DACEND010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.002"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(115..1506)
                     /locus_tag="IW746_000080"
     CDS             complement(115..1506)
                     /locus_tag="IW746_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1077012.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1719..2105)
                     /locus_tag="IW746_000081"
     CDS             complement(1719..2105)
                     /locus_tag="IW746_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1077013.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2161..2784)
                     /locus_tag="IW746_000082"
     CDS             complement(2161..2784)
                     /locus_tag="IW746_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1077014.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNKLFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2848..3516)
                     /locus_tag="IW746_000083"
     CDS             complement(2848..3516)
                     /locus_tag="IW746_000083"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1077015.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3528..3968)
                     /locus_tag="IW746_000084"
     CDS             complement(3528..3968)
                     /locus_tag="IW746_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077016.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4027..4698)
                     /locus_tag="IW746_000085"
     CDS             complement(4027..4698)
                     /locus_tag="IW746_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1077017.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4883..5281)
                     /locus_tag="IW746_000086"
     CDS             complement(4883..5281)
                     /locus_tag="IW746_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1077018.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5278..6051)
                     /locus_tag="IW746_000087"
     CDS             complement(5278..6051)
                     /locus_tag="IW746_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1077019.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6048..6731)
                     /locus_tag="IW746_000088"
     CDS             complement(6048..6731)
                     /locus_tag="IW746_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1077020.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6898..7785)
                     /locus_tag="IW746_000089"
     CDS             complement(6898..7785)
                     /locus_tag="IW746_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077021.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7782..8651)
                     /locus_tag="IW746_000090"
     CDS             complement(7782..8651)
                     /locus_tag="IW746_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077022.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8788..10074)
                     /locus_tag="IW746_000091"
     CDS             complement(8788..10074)
                     /locus_tag="IW746_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1077023.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10347..10925
                     /locus_tag="IW746_000092"
     CDS             10347..10925
                     /locus_tag="IW746_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1077024.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10925..11854
                     /locus_tag="IW746_000093"
     CDS             10925..11854
                     /locus_tag="IW746_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077025.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11851..13485
                     /locus_tag="IW746_000094"
     CDS             11851..13485
                     /locus_tag="IW746_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1077026.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSINQLLSNAQHHYEQTIQD
                     EKKKRSLEIQTMQAQIQPHFIYNTLTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKK
                     EEVIPVKEEIKSVQSYIDLLSMRYGERISAKIMIPDELQSLSMPNMILQPIIENAYLH
                     AFHNKTQGFITVYGKTKNQLLLFEVIDNGDGFDTENTKQKNDHFSGIGISHVDERIRL
                     MYGDAYGLTIQSVIGTGTTVTICLPLLPKNQSEYQK"
     gene            complement(13548..14384)
                     /gene="pheA"
                     /locus_tag="IW746_000095"
     CDS             complement(13548..14384)
                     /gene="pheA"
                     /locus_tag="IW746_000095"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1077027.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14381..14890)
                     /locus_tag="IW746_000096"
     CDS             complement(14381..14890)
                     /locus_tag="IW746_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1077028.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(14883..16178)
                     /gene="aroA"
                     /locus_tag="IW746_000097"
     CDS             complement(14883..16178)
                     /gene="aroA"
                     /locus_tag="IW746_000097"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1077029.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16191..17282)
                     /locus_tag="IW746_000098"
     CDS             complement(16191..17282)
                     /locus_tag="IW746_000098"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1077030.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17299..18465)
                     /gene="aroC"
                     /locus_tag="IW746_000099"
     CDS             complement(17299..18465)
                     /gene="aroC"
                     /locus_tag="IW746_000099"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1077031.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18467..19531)
                     /gene="aroB"
                     /locus_tag="IW746_000100"
     CDS             complement(18467..19531)
                     /gene="aroB"
                     /locus_tag="IW746_000100"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1077032.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19564..20586)
                     /gene="aroF"
                     /locus_tag="IW746_000101"
     CDS             complement(19564..20586)
                     /gene="aroF"
                     /locus_tag="IW746_000101"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1077033.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21245..22219)
                     /locus_tag="IW746_000102"
     CDS             complement(21245..22219)
                     /locus_tag="IW746_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1077034.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22200..24083)
                     /locus_tag="IW746_000103"
     CDS             complement(22200..24083)
                     /locus_tag="IW746_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1077035.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24101..26326)
                     /locus_tag="IW746_000104"
     CDS             complement(24101..26326)
                     /locus_tag="IW746_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1077036.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26441..27475
                     /locus_tag="IW746_000105"
     CDS             26441..27475
                     /locus_tag="IW746_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1077037.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27542..28555)
                     /locus_tag="IW746_000106"
     CDS             complement(27542..28555)
                     /locus_tag="IW746_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1077038.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLIWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28633..28821)
                     /locus_tag="IW746_000107"
     CDS             complement(28633..28821)
                     /locus_tag="IW746_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1077039.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29261..29389)
                     /locus_tag="IW746_000108"
     CDS             complement(29261..29389)
                     /locus_tag="IW746_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1077040.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29392..30765)
                     /gene="rlmD"
                     /locus_tag="IW746_000109"
     CDS             complement(29392..30765)
                     /gene="rlmD"
                     /locus_tag="IW746_000109"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1077041.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30876..31931)
                     /locus_tag="IW746_000110"
     CDS             complement(30876..31931)
                     /locus_tag="IW746_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1077042.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(31941..33371)
                     /gene="gatB"
                     /locus_tag="IW746_000111"
     CDS             complement(31941..33371)
                     /gene="gatB"
                     /locus_tag="IW746_000111"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1077043.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33368..34840)
                     /gene="gatA"
                     /locus_tag="IW746_000112"
     CDS             complement(33368..34840)
                     /gene="gatA"
                     /locus_tag="IW746_000112"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1077044.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGNPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34840..35145)
                     /gene="gatC"
                     /locus_tag="IW746_000113"
     CDS             complement(34840..35145)
                     /gene="gatC"
                     /locus_tag="IW746_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1077045.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35251..37287)
                     /gene="ligA"
                     /locus_tag="IW746_000114"
     CDS             complement(35251..37287)
                     /gene="ligA"
                     /locus_tag="IW746_000114"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1077046.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37304..39538)
                     /gene="pcrA"
                     /locus_tag="IW746_000115"
     CDS             complement(37304..39538)
                     /gene="pcrA"
                     /locus_tag="IW746_000115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1077047.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39693..41252
                     /locus_tag="IW746_000116"
     CDS             39693..41252
                     /locus_tag="IW746_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1077048.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41363..43294)
                     /locus_tag="IW746_000117"
     CDS             complement(41363..43294)
                     /locus_tag="IW746_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1077049.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43312..44226)
                     /gene="pfkB"
                     /locus_tag="IW746_000118"
     CDS             complement(43312..44226)
                     /gene="pfkB"
                     /locus_tag="IW746_000118"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1077050.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44223..44975)
                     /locus_tag="IW746_000119"
     CDS             complement(44223..44975)
                     /locus_tag="IW746_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1077051.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45134..45343)
                     /locus_tag="IW746_000120"
     CDS             complement(45134..45343)
                     /locus_tag="IW746_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077052.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45354..46682)
                     /locus_tag="IW746_000121"
     CDS             complement(45354..46682)
                     /locus_tag="IW746_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077053.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47047..47634)
                     /locus_tag="IW746_000122"
     CDS             complement(47047..47634)
                     /locus_tag="IW746_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1077054.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47717..48967)
                     /gene="clpX"
                     /locus_tag="IW746_000123"
     CDS             complement(47717..48967)
                     /gene="clpX"
                     /locus_tag="IW746_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1077055.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49214..50503)
                     /locus_tag="IW746_000124"
     CDS             complement(49214..50503)
                     /locus_tag="IW746_000124"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1077056.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50657..51589)
                     /locus_tag="IW746_000125"
     CDS             complement(50657..51589)
                     /locus_tag="IW746_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077057.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51746..52405)
                     /locus_tag="IW746_000126"
     CDS             complement(51746..52405)
                     /locus_tag="IW746_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1077058.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52408..53433)
                     /gene="sppA"
                     /locus_tag="IW746_000127"
     CDS             complement(52408..53433)
                     /gene="sppA"
                     /locus_tag="IW746_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1077059.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53446..53736)
                     /locus_tag="IW746_000128"
     CDS             complement(53446..53736)
                     /locus_tag="IW746_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077060.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54145..55950
                     /gene="glmS"
                     /locus_tag="IW746_000129"
     CDS             54145..55950
                     /gene="glmS"
                     /locus_tag="IW746_000129"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1077061.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        15641 a        12064 c         9944 g        18357 t
ORIGIN      
        1 cagaagtagc ttctcggaaa taagccgaaa tttcacaaaa atttgagaaa ctatttttag
       61 gaaacttctt cttatttctt aaagctgacc acttctatca caacctcttt ttgactatgc
      121 attcacatcg atcactttag aatcatcatt gatgacatcg cgatctaaat ctttgatgcg
      181 tgcacttgtc acgataccag ctaccatgct accgctgacg tttactgctg tacgtgccat
      241 atcgattaat ggttcaacag agataactaa cccaacgatt gctactggca agttcattgc
      301 ccctaaaacg atcagtgaag caaatgttgc gcccccaccg actccagcta caccaaaaga
      361 actgattgtt acaattgcca caagcatcaa aataaattcc aaactaaaga cattcactcc
      421 agctgctggt gcgacgatcg ttgccaacat agctggataa acgccagcac agccgttttg
      481 tccaatagac aaaccaaaac ttgctgagaa attagctgtt gcatcatcta ctcctaaagc
      541 ttttgtctga gtctcgatat tcaatggcaa agctccagca cttgaacgtg aagtaaacgc
      601 aaagctcaat accggaaagg cttttttgaa atagttgatt ggattgactt ttgcaccaat
      661 caaaatcagt gagtggacaa tcaaaacagc aagcaatgct acgtaagaag caacgacaaa
      721 tttacctaaa ttgacgatag ctgcaaaatc acttgttgcc aaaacgtttg tcatcaaagc
      781 aaatactcca taaggtgtca aacgcaaaac aagcgtcacg atccgcataa tgattttata
      841 cagactgtcg actaaatctg aaaagaatgc tgcttctttt ggcgcttttc ttttcacgcc
      901 tagataagcg attccgacaa atgccgcgaa aataacgact ccaatcgtac ttgttgcacg
      961 actacctgct aaatcagcaa aaacgttttt agggataaag gataaaatct gctgtggaat
     1021 cgataagttt tcaactgttt cctgacgtgt tgccagttca gagattcgtg ctgtttctgc
     1081 agctccttgt gtgaatttag ctccttggag tccgaacagc attgttgaac caattccgat
     1141 caatgctgca atagctgttg ttccaagtaa agaagtcaat acagtcgcac taattttccc
     1201 tagcttttca gaaactttca tttttgtgaa tgcaccaatg atcgaaataa acactaaagg
     1261 cataactagc atttgcaaga atgatacata tccttgacct acaatcccga tccattccat
     1321 tgattgagta acgactttgt cgtctacacc aaagatcaat tgaagcagac cgccaaagac
     1381 gataccaact cctaatgctg taaagacgcg tgttgaaaat tttgtatggc gcttttgcat
     1441 acgatagaac acatataata atgccgcaaa aactagaacc actaaaactg tgatgagtgt
     1501 tgtcattttt ctcctcctca tattattaat gacaagtcca tcgaaggaag taaaggttcc
     1561 cgagaatgat tcttcaacca tcctcctgaa aaaatttttt tcagtgactt ttatatttta
     1621 gacgattttt atcgaaatga cattcttttt gcttacaatt agtaataaaa aaacatacct
     1681 gtgatagctt taatctatcg caggtatgtt tttactgtct attcttgttt ttctgattga
     1741 aaattcaatt ttaccccagg ctgatctaag gcgatctctg tcacctcata agacattcgg
     1801 cgtacgatat cgaataacgg tgctactagc tcgtccactt cttcttgtgt gacatcattt
     1861 tgaccttcaa ttttccgatt gaggatatga ttgatctgac tcacacttcc agataaaaca
     1921 tatgtatcaa acactaaacg gaactctaag cgtgctccta cgatgctatg ttctttcgga
     1981 tagttttcga tcgtttcttt taaaggcgag aagcctactt tcaaatctgt ttctacttct
     2041 tggttcggca atctcatatc ataatgaaat gcttctacaa cttctttttg tcttctgatt
     2101 tccatataat tttatcactc ctttatgaaa attataacat atctcctttg aaaggggata
     2161 ctacttttta atgatgctct gcaaggtaag gatttcattc agatttccta ccacataatc
     2221 tgccgggatc tcacccagaa aatgatcgat gtcgtaaaga caagtagcga ctcctgcatt
     2281 tttccctgct tcgatatcta aacgacgatc gccgatcatc atcgtgtctg ttctttctaa
     2341 atgaaaagta tcgatcaaat aatttaaaga tgctggatca ggtttacgag gaaagtcctg
     2401 atcgatccct actacttcct caatcagatg tgccaatcga tgttctttta acaggcccca
     2461 tgttgattct gtcaaacggt gggtcaggat gaaatgacga ccgccgttgt cttttagcat
     2521 ttccagtact tgtttcgttt ctggaaatgg acgggaaact tccgtacttt ctttttcata
     2581 tcggtgaaat aatttattaa actctctggc attcaagttt gattcattga tcaaataact
     2641 agtagaatac tttttcattt tgaagtagat ttcttttttg tccatactga taccaaaatc
     2701 ttttagtgct tgccatgcac catctaccat tgcgggatac gtatcaaaca atgtaccatc
     2761 aaagtcccat atataatttt tcatgttcct tttgttccat tcctttgctt cttttttcta
     2821 tctctcactt ttagataaag gtgttcttta aagtattttt ttgtccagaa ttgtcaaatc
     2881 atctgctaat gtatactgga ttggttgtgc atttgggata tcgatcgtat ccatttgatc
     2941 ttcaggaaca tcttctaaat attttgtcag tgctcggatg ctattaccat gacccacaac
     3001 tagtaaatct tgccgatctt ttagttttgg tgctagatga tcttgccata agggaatgat
     3061 ccgattcact gtcatttcca gactttctcc gtatgggatc aaatgcggat ccaagtgatc
     3121 atatctgcgg tcaaatctat tttctgcggt taacggcgga acttccgtat atcctctgcg
     3181 ccatgttttt acttgatctt ttccgtatat ttctttcatc ttatctttgt ttttacccac
     3241 aagatcccca taatgccgtt catttagacg ccatgttttt attataggaa gccataactg
     3301 acccatttct tctagtgcga cttgtccggt aagaatcgca cgtttcaaaa cagaggtata
     3361 aacggcatcg aattgaagtc cggcttcaag cattttttga ccggcaactg ctgcttgttc
     3421 ttttcctttt tctgttaagg cgacatctag ccatcctgtc cagtaatttt caaaatttgc
     3481 ttcgctttcg ccatgacgta ataatactaa tttcattctt ttctccctta aacattcgtt
     3541 tcgaatttta taccacatgt tttgcatgtc acacgtattc gtccttttcc tttaggtgca
     3601 cgctgctttt gatgacaatt cgggcactca aaaaaagtat aactttttcg atccattttg
     3661 acttgctttt tgttttttat ctgatgatat ttttctttga ctctatcaag atatttcaaa
     3721 taacgttgat tctcatttag tctggggtag attttttttg ataggaaacg aaatatcatc
     3781 catccgaaaa agacgatgaa caacaattgt ccgattctat agggcaacca acgacttagc
     3841 aaactaaaga caagcgcagc gatcaataga ccgcgattca actgatcaaa tttcgcatat
     3901 cttccctgca taagtttgtg atagttctgc tgaaatcgta ttagcctttc taaccatgtt
     3961 tgcatcatat tatctcctcc atcatctaat atttattatg acaaaaaaat ccaaaaagaa
     4021 aaagccttaa ttggcctttt ccattgcaac ttggtgaagg acattccgat ttctggtaaa
     4081 gtcaatcaca atcactttct ttttatcttc tctgttgact actcgaaatg tgtccgtcaa
     4141 tggaagaaac agcttcttag atggtagctg ttgcatacgt ataaaacgat ggatcatcgt
     4201 aatcaattga ggaaattgaa tagacgtgtg aagcagacct ggatattttt tcaacagctg
     4261 tgtcgttttg atcagattcc gttttttact gatcactttt aaaaagcttt ttagaatctt
     4321 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4381 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4441 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4501 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     4561 atagatttct cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt
     4621 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     4681 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     4741 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     4801 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     4861 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     4921 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     4981 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5041 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5101 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5161 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5221 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5281 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5341 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5401 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5461 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     5521 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     5581 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     5641 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     5701 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     5761 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     5821 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     5881 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     5941 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6001 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6061 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6121 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6181 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6241 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6301 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6361 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6421 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6481 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     6541 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     6601 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     6661 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     6721 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     6781 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     6841 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     6901 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     6961 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7021 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7081 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7141 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7201 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7261 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7321 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7381 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7441 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7501 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     7561 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     7621 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     7681 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     7741 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     7801 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     7861 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     7921 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     7981 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8041 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8101 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8161 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8221 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8281 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8341 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8401 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8461 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     8521 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     8581 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     8641 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     8701 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     8761 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     8821 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     8881 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     8941 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9001 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9061 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9121 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9181 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9241 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9301 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9361 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9421 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9481 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
     9541 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
     9601 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
     9661 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
     9721 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
     9781 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
     9841 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
     9901 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
     9961 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10021 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10081 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10141 aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac
    10201 aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg
    10261 tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca
    10321 agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac
    10381 agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa
    10441 tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata
    10501 tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa
    10561 aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg
    10621 ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg
    10681 atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa
    10741 gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac
    10801 aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac
    10861 cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc
    10921 attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc
    10981 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
    11041 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
    11101 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
    11161 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
    11221 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
    11281 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
    11341 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
    11401 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
    11461 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
    11521 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
    11581 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
    11641 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
    11701 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
    11761 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
    11821 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
    11881 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
    11941 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
    12001 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
    12061 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
    12121 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
    12181 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
    12241 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
    12301 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
    12361 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
    12421 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
    12481 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
    12541 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
    12601 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
    12661 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
    12721 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
    12781 gatcgacaca caaaatcgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag
    12841 acgatccaag acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata
    12901 cagccccatt ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa
    12961 acagatgaag cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg
    13021 aaaaaagaag aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat
    13081 cttttgagta tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg
    13141 cagtcattat ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat
    13201 gcttttcaca ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt
    13261 cttttattcg aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa
    13321 aatgatcatt tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat
    13381 ggcgatgcat atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt
    13441 tgtctaccgc ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc
    13501 tgattttttt attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc
    13561 tcttctactg tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg
    13621 atttcttgga gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg
    13681 aaaaaatatt ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt
    13741 tcccgccagc caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt
    13801 gtcaagatga cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg
    13861 acgatccaaa aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc
    13921 aatccaacat gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt
    13981 tctgctacat acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga
    14041 taatgcgttt ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt
    14101 gtaatctttg ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt
    14161 ttaacttcga cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc
    14221 aatgaattct ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa
    14281 caaacaggga tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc
    14341 tgataagtga atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt
    14401 ctataatggt atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg
    14461 cactttcttc ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt
    14521 ttccaagagc taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt
    14581 ccgcttctgc ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg
    14641 cactttggac aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta
    14701 aaatatctga ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa
    14761 tcgacaattg gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac
    14821 attgagctaa ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa
    14881 tgctagccat gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga
    14941 taagagattg aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca
    15001 gctacttgca gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc
    15061 agaggtgtcg gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt
    15121 ttctgtaatt cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct
    15181 gcgtctttga tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat
    15241 tcatcaatca gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc
    15301 ttgacactta aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg
    15361 cccatctcct ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt
    15421 aacaacagtt ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa
    15481 atatcacctg gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc
    15541 gttttatctt ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta
    15601 cgggaacgtt ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc
    15661 aaagcagatt tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag
    15721 cgggttcctg tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg
    15781 cccatctcgc gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc
    15841 ccgaacagag tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc
    15901 gtaccggagt ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga
    15961 ccatgaatct tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca
    16021 tgtaaagtac tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca
    16081 gcaattgctc caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca
    16141 gtccctctta atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc
    16201 cagcaatgat aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg
    16261 tttttaaaac tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa
    16321 ttaatcaaag aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt
    16381 tcatctggca catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca
    16441 ggcaactggt ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc
    16501 tcagtcatga tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc
    16561 aataatatct gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg
    16621 atcctagtga tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga
    16681 ttgaattgtt ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc
    16741 attcctgtta tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg
    16801 caaaatagtt cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaaaatg
    16861 taataggcat tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca
    16921 gccattggat gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc
    16981 tgcttcgtac tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg
    17041 acatcaagat aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt
    17101 tctgcacctg tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc
    17161 tcacgggttt ttgactgact atcccaacca actactatca gttctggatt ggtctgacga
    17221 atacaaagtg ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc
    17281 atcttatttg cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc
    17341 tttcaactca acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt
    17401 ggctacaaca ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc
    17461 tacacttgct ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa
    17521 tgtaggaatg ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc
    17581 ttcgaagccg cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat
    17641 ctcatccatc acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac
    17701 gcctttaaag gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc
    17761 ccattgtacg tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc
    17821 tccgatcgta tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac
    17881 tgctggatca acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga
    17941 gatatttcca ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat
    18001 gatgtcaagt tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt
    18061 ctctcttgca gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc
    18121 gcctaccaaa tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt
    18181 ttcaggcact tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac
    18241 cactaaagtg acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac
    18301 acgatctttt tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag
    18361 ttcgtgattg atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc
    18421 tgttaattct ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta
    18481 agtaatcttt gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta
    18541 gcaagatgat attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata
    18601 attcatctgg atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt
    18661 ctaaagcagc tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg
    18721 ctaccattcc aatagctact ccttctccat gactgactac cccatatcct gctgtatttt
    18781 caattgcatg gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga
    18841 catcttcttc cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat
    18901 gttccagaag atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg
    18961 ctatagctgc tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag
    19021 tttttagaac ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt
    19081 ttttcgcttt gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa
    19141 gtagcgtagt cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg
    19201 ctgctagatc acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat
    19261 cattatctgc taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac
    19321 ccgccggtac gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt
    19381 tttctgcata tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc
    19441 aaatcgtttg aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt
    19501 atttttggtt atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg
    19561 gaattaatcg agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat
    19621 catgcgtgag taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg
    19681 atcaggatgg atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat
    19741 tggtggtaca agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg
    19801 agttaatttt ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc
    19861 gtaagtccga atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta
    19921 ttctgcagca ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg
    19981 tttacctgtt ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat
    20041 ctgtaggata tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt
    20101 aatcactttc attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc
    20161 taaaccttgg aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt
    20221 tgcgcccccg gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga
    20281 acaaggacca gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac
    20341 gactgtattg ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac
    20401 agcattttct actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc
    20461 ttttagtcca atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt
    20521 ttcgatatga tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac
    20581 tatcattgtt tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact
    20641 ttttcaaggt ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt
    20701 cgctccttct ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa
    20761 attgcctcta cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata
    20821 gccactatag atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc
    20881 atagatacaa acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat
    20941 agctaaagta atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca
    21001 aactgatgta agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta
    21061 tgaaactttt tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt
    21121 ttattgaaga aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat
    21181 gacatataga ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag
    21241 attgctactg aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg
    21301 actcaacatc cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg
    21361 taagaaccgt tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt
    21421 ctgctttgaa tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg
    21481 aaaaatcttc ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc
    21541 atttcgtttc catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc
    21601 ctgccacttt ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg
    21661 ataggccttc atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg
    21721 cgggtaattc cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct
    21781 ctccagcgat ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg
    21841 taaaaatgat ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag
    21901 caatcggcaa atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat
    21961 acaatccggc tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat
    22021 cggtcgttgc tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt
    22081 tattgagcga ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt
    22141 tgacacctaa tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt
    22201 tatttactgt atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca
    22261 tctgcaaaaa cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca
    22321 ccgccatagc gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct
    22381 ttaaaatccc atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa
    22441 gaatccattc cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt
    22501 ttttccgctt tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt
    22561 tttacatctg aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca
    22621 agtcgatcgg ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca
    22681 taagattcat ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa
    22741 ttcgtttttg ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg
    22801 taagaaggct caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc
    22861 gccatcacat ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa
    22921 tgagatgtcc gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata
    22981 tcttctatag tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg
    23041 ataatcatcc gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa
    23101 ccgaaggaat agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca
    23161 agcttatata aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg
    23221 cgaatcgtca tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga
    23281 tctagtatcg ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact
    23341 tcaagtttgc cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa
    23401 tggttttgtc ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag
    23461 tcctgatctt ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt
    23521 tcttcttcca aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac
    23581 ccgataaaat gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc
    23641 gagatttgtt ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc
    23701 ggattgtctt tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa
    23761 gggggacggc gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg
    23821 tcataatttg ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca
    23881 aaatcttttt tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc
    23941 catgatccat ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca
    24001 taacaaacat gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct
    24061 aaccatttta tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa
    24121 gcagttctgt ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt
    24181 cggcccccga aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc
    24241 ccatcagata tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca
    24301 caatagcttc atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag
    24361 gactcgccaa acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata
    24421 taatttggtc ttttacttgc ttttcttctt caggagacag ctttgtcaaa tccagttcat
    24481 aaccaaaaac agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg
    24541 tctgatgatt tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc
    24601 ttgttccata ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact
    24661 gcggcatata ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc
    24721 ataagatttc cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat
    24781 aacgatgcgc tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa
    24841 gcgaacggtt catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca
    24901 tttgtttctc aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc
    24961 tggaagcaga aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca
    25021 gttcagaatc aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc
    25081 tatgagcata atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc
    25141 agtctcctaa tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga
    25201 acatttcgat gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga
    25261 agtcaaagta agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac
    25321 gacctcttgc gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc
    25381 cctgacttga gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt
    25441 cccacgaaaa acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt
    25501 tttcaagttc gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag
    25561 tattttctgt cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg
    25621 ttcctcgacg gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc
    25681 tttcattgac atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa
    25741 agtctagctg gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta
    25801 tctttacaga tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat
    25861 aaatcgctgt ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca
    25921 tcacataagt ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt
    25981 catatctcag atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc
    26041 gaaagtccat gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc
    26101 ggtcaggatt taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat
    26161 tgtctttgta ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt
    26221 ctatctttat cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat
    26281 caaataagat gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa
    26341 caaaacggaa accgtttact tttttttata attcctgtat actgtttact aaacaatttt
    26401 gtcaataaaa attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt
    26461 cgcacgaaag gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac
    26521 gatccatgtg gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta
    26581 tgacatcagt actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac
    26641 gatcggcaaa gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga
    26701 agaagaagct cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc
    26761 taaacaatgg gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa
    26821 agaatcgatt accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga
    26881 catccagcaa gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact
    26941 ggaattgttc ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga
    27001 tatggatgaa ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca
    27061 gcattttatg atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac
    27121 agaaaaacaa ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac
    27181 tgccattctt gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa
    27241 aaatatccaa attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc
    27301 aaacctctcc cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc
    27361 tgttcgaatg gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta
    27421 tcctgcacga ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag
    27481 aggttgtgaa aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct
    27541 cttacttata cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta
    27601 gcgtgtaatc aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg
    27661 attgcacggg tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga
    27721 atgagtatgc atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag
    27781 ccacactagc ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc
    27841 acgcaacggt tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta
    27901 gcttcaaatc aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta
    27961 gctgtttgat tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca
    28021 atgcgatttg tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt
    28081 tgtcgtcttt ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat
    28141 tagggtattc tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt
    28201 ctgaaactaa ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc
    28261 caccatgtac ccaaattaga acaggaacag gccccttgct gttttttgga tagtagatgt
    28321 cataagtatt ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt
    28381 tttctgcttg atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt
    28441 tgatcaagaa tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga
    28501 tcccaagaat gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc
    28561 tccctcagta gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct
    28621 ctcctagatg atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa
    28681 ttttttccat tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg
    28741 aggacttaag cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat
    28801 cacttgctgg tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta
    28861 atttatcttc agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt
    28921 ctaaataata ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc
    28981 cccctgatgc cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg
    29041 aatcatttga gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat
    29101 aacgaaaaat ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc
    29161 aacagtcctt attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca
    29221 ataacaaaag ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag
    29281 ggccttttct catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc
    29341 caccttgttt ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc
    29401 tttaacgaat gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg
    29461 aactttcttc acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc
    29521 cggattacat gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa
    29581 atgatgatct aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt
    29641 ttcttgccat tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat
    29701 tttattattt aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc
    29761 atacacctta tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc
    29821 gtcgatcaca atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata
    29881 ctctgcttgt tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc
    29941 taatagctga tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat
    30001 cacatttgtt ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc
    30061 atgaatagct tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac
    30121 gaccatcatt tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc
    30181 atttttttct tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt
    30241 gattgcttga tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg
    30301 gctattcttt ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg
    30361 tgctttatta cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc
    30421 tggcatctta gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg
    30481 ttgttcatac ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc
    30541 tacaggctga cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt
    30601 attgcctgct tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa
    30661 taatggataa ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc
    30721 gactgtatat cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa
    30781 gcctattttt caatcctact gaactctact ataaatcagc taattacaca actaaaaccg
    30841 gcactgacct tactacgttc agcaccggct tttgattaat tcttttgatt aggtgtttcg
    30901 tctgctattt ttccgtcacc gtcgatatct tcatccgtta aacgttcgac ttcttttacg
    30961 aattctttgc tgacaacctc taattcttct tcatctgtac ctgtgattgc ttcatcagga
    31021 atctgatcgg tattggcaaa gaactcaatg tgttgatgaa gattttggaa atgcatcggt
    31081 gcttccccgc cgtattcacc atctaagttg atcattactg gtcgattgat atcatcttct
    31141 gtttcaacga acaaactgct tgttttggta tagattattc ttggatcatc gacatgtcga
    31201 ccgccattca gcatgagtgc tgcaatatgc aagatctcaa agatatttgc tgtcttcaca
    31261 atgattaaag aaaacttccc atcatccaat ttggcatctg gcgcgatctg ttcgaatcct
    31321 ccaacagagt tcgtcagccc taggaaaaac atcgaagcat ttccgatata ttccccttca
    31381 tcatacttca agcgcatttt gattggcttt actcgaggga gcatctctgc tcctttcgca
    31441 agataagcaa gataaccaaa aatactcttt agttctgatg gaacttcata agtcaattct
    31501 gtcaaatagc cacctgcagc gatattgatg aagtaactgt tatcagaaag gccgatgtcc
    31561 atttttacgg tttggtgttt ttttatcact tctgctgctg ctttaatatt atctctagga
    31621 atttttaatg ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc cattttagga
    31681 cggtgttcaa gcggagcgat tccattaacc acttcgttga ttgtgccatc gccaccagct
    31741 gccactacta gatcaaaacc tagttctgcg actctacatg cttcattctt ggctgaatct
    31801 ggttctggtg tagttgcata ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg
    31861 atatccgcca aatttttttt caataattct tttcccgagg tggggttata aattacgcgt
    31921 gcttttttca ttttcagcct ctatcgtttc gctaattcat ctttaagtag cttattcacg
    31981 acaccaggat ttgcttgtcc tttagttgct ttcatgattt gaccgactag gaagccaacc
    32041 gcacgatctt ttccattttt gaaatcatcg acagattgtt ggttgttatc caaaatttca
    32101 ttaatgattg gcagtagttt gttcggatca gataattgca cccaaccatt tttttcaact
    32161 acttcttggg cttctccgcc atttgtgatc aattcacgga agactttttt ggcgattttt
    32221 gagctgatcg ttccatcggc gatcaaacga atcattcctg ctaagttctc aggtgtcaat
    32281 tttgtctcag ctaattccag tttctcactg ttcaagtaag cagaaacttc acccatcaac
    32341 cagttagatg cttgcttggc atctgcacca ttttccaaaa cagcttcaaa aaaatcagac
    32401 atttcttttg taagtgtcaa taccattgcg tcatactctg gaagtccaaa ctcacggata
    32461 tagcgttcgc gacgagaagc tggcatctca ggcaaactag cttttacttt ttgaatccat
    32521 tcgtcatcga tcacgaattt cgggatatca ggctctggga agtaacgata atcactagac
    32581 ccttctttga cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct
    32641 tgttggattt ctcctcctga aagaagaact tttgcttgac gtttttcttc aaaagctaaa
    32701 ccttttttca cgaaagataa agagttcaag tttttcaatt ctgctttcgt accgaactct
    32761 tcttgtccat aagggcgaag agaaatattg gcatcacagc gcatagagcc ttcttccatt
    32821 ttcacatcgc tgacttctgt aaattggata attgatcgca atgcttctag ataagcgtat
    32881 gcttcttctg gtgaacgcat atcagcttca gaaacgattt cgatcaaagg cgtgccttgt
    32941 cggttcaaat caacgtaaga ataaccgcca ataccatgca tgttttttcc agcatcttct
    33001 tctaaatgga cacgttcgat ccggattttt ttctttttac cttctacttc gatttcgatc
    33061 catccgtcat gaccgattgg ttgatcaaat tgggaaattt gatatgcttt tggattatct
    33121 gggtaaaagt aatttttccg gtcaaaatga gtatctttag agatttcaca gtttaatgcc
    33181 aaagctgctt tcatcccgaa ttccagcgca gctttattca ttactggcag cacgcctgga
    33241 taaccccagt cgatcacgtt tgtattgctg tttggttctg caccaaaatg agctggcgct
    33301 ggagagaaaa tttttgagtt ggtttttaac tcgacgtgga cttctagtcc aatgactgtt
    33361 tcaaaattca ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg aattctgttg
    33421 cttgttcaaa cgcatatgcc gcttgataca tcgttttttc atcaaaataa ttaccgatga
    33481 tctgcaatcc aactggtaat ccttctgata aacctgccgg tacagacata cctggaagtc
    33541 cagccaagtt gacagggatc gttaagatgt cactcatgta cattgtgatt ggatcgttaa
    33601 tgttctcacc gattccgaat gcaactgttg gtgaagatgg cccgatgatc aagtcataat
    33661 ctgcaaagac tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct gcttttttga
    33721 agtaagcgtc ataataaccc gcgcttaagg agaatgtacc taacatgata cggcgtttta
    33781 cttcttcacc gaatccttct gaacgtgagt tgacatatac atcatctaat gatttcacat
    33841 tttcagagcg ataaccgtag cggataccat cgaatcgttg caaattggaa cttgcttctg
    33901 atgaggcaat gatataataa acagccacac cgtattttga atgaggcaag ctgacttctt
    33961 ccacaacagc acctaatgaa cggaatgttt ctgctgcttt caatacagct tcttttacgc
    34021 caggggccac accttcgcct aagtattctt taggtaaagc aatcttcatt cctttgatgt
    34081 cgcctgtcag tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt
    34141 cgtcataacc gctgatagct gtcaatgcta atgcattatc tttcaccgta cgggtcattg
    34201 gtccgatttg gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag acacggccat
    34261 aagtaggttt catccctacg atgccgttaa aagctgctgg ttggcggata cttcccccag
    34321 tatcactacc taaagaaact ggaatttgtc cagctgcaac tgctgctgca gaaccaccag
    34381 aagagccacc aggtactttt gttgggtccc aagcattttt tgttttcttg aaataagaag
    34441 tttctgtact tccacccata gcaaattcat ccatgtttag cttaccaaca gggatcatat
    34501 ctgcttgata gattttatcc atgaccgttg catcataaat tggttcaaaa ttgtaaagga
    34561 tcttagatgc agcggttgtc aagatatctt ttgttacgat attatccttg ataccaattg
    34621 gattaccagc aaggacgttg ctttcatcaa tccctttttc atctaaagct tttgcctgtt
    34681 ccaatgcttt ttcttcgttc aatgtaatga atgcctcgac ctcaggctct gtttctttga
    34741 ttcgttcaaa tgttgttttt gtcaaatctt gtacagtgat ttctttcgat acaagcatat
    34801 gatgcagttc ttctagagaa cgattgtgta attctgtcat tatgcgccag cctccccatt
    34861 atcgatgatt gcgggtactt tgataaagcc gtcttctgtt tccggcacat ttttcaataa
    34921 ttcatcgcgg ttccatccag cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac
    34981 cacattcgac gtaaatggaa caccttccgt atctacttct tccagcattt caaccatatc
    35041 gatgatttta cctaattggt cggtaaaact tgcaagttct tcatctgcga acgcaagttt
    35101 tgctaatttg gcaacgtgtt tcacttgttc ttcactaatc gccatactac cccttctttc
    35161 taaacgagtt aaaaactttg ctctctattt taacacaaga gtgccgtgtc tgaaattgtt
    35221 tattgaaatt tgtctgaaaa aactcatttt tcattcttct actgagcgac ttttagccaa
    35281 tgcatctgcc atttgatctt ctgtccaaac ttcgattcct aattcttgtg ctttggtcag
    35341 cttgctgcca gcatcttctc ctgctaccac gatatccgtt tttttcgata cgctaccagt
    35401 cactttcccg ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag
    35461 tttaccagta aggactactg ttttttcttt aaaaggagac tcgacttctt gcaattgggc
    35521 atttcgaagc cctttgtatt cgaaattgac gcctgcttgt tttaattcat tcatcaattc
    35581 atggacttct tcgttactga aataagtgac tacactgtcg gcaatgatat caccgatcgt
    35641 atcaagcgca ataatcgact catagtcact tttactcaat gtttctagat caccgaaatg
    35701 ttctgctaaa atttttgctg ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa
    35761 ccgctcgacc gaattatctt tgctgttgtc gattgctgtc aaaatattat ttgctgattt
    35821 ttcttttatt ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa gatcagcaac
    35881 atctgctact agttttttat catacatctg ttctagtacg cgcggcccca acccatcgat
    35941 attcatcgca tttcttgaaa caaagtggtt caaaccttct ttcatttgag cagggcattt
    36001 aggattgatg caccgtaagg ctacttcttc atccaaatgg acaagttcac ttccacaaac
    36061 tggacaatgt gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat ctaacacgac
    36121 ttgcgaaact tctgggatga tgtctcccgc tttataaatc agaactgtat ctttcaagcg
    36181 gatatccttc aacttaatat aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc
    36241 tgctactcgg actggtgtca tgatagctgt tggcgttact acccctgttc gaccgactgt
    36301 ccattcgatg ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc
    36361 ccatctcggt gctttgatag taaaaccaag ttgatcttgt agcgagaatt cattcacttt
    36421 gatgacgatt ccatcgattt catagggcaa atccacacgt ttgtcatgat attcttcaat
    36481 gtatgaccac acttcatcaa tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc
    36541 aattttttct agctcttcta gcgcatcata ctgggttttt gcttgcatag gaccgaaatc
    36601 agctactgtg tataaaaaag tgtccagatt tcttttcgct gtgattttag aatctaactg
    36661 acgcaaagaa ccagctgcgg cgttccgagg attcgcaaag atatcttttc cctctgcttc
    36721 tctgtcttgg ttcaattgga caaatgaccg ttttggcatg aaacattcgc ctcttacttc
    36781 gatgttcatt ggttctttta atttgattgg gaccgaccgg acagttttta ggttttcagt
    36841 gatgttttct cctacagttc catccccgcg agtcgcacct ctcacgaaat ttccatcttc
    36901 ataacgcaaa gaaacggaaa gtccatctat ttttagttca cagcaatagt caactacacg
    36961 tcccactgct ttttgtacac gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc
    37021 gttcaaactg taaagcggaa tatcatgagt gactttttca aaaccttcta atacttttcc
    37081 gcctactcgt tgagtgggag aatcagacgt gatcaaatca ggatattgtt cctcgattgc
    37141 tactaattct gcgtattctt tatcataaac atagtcttcc actgttggtt tatcttctac
    37201 gtagtattcg cgagaccatt ggttcagtct tgtacgaagt tcttctgctc gtttagcagc
    37261 ttcgtcaatc gtcatttcat gttccatcat ttaccctcca ctactaagct ttttcaattg
    37321 gcgcaaaggc agccaaaaga cgttttactc cttgacttgg gaaagccaca tctagttcta
    37381 gatccttcga cgtcccgctt actcggacga ctgttcctag accccatttt ttgtgtttga
    37441 ctttatctcc aggtttccag ctgttatttt cccctccagt agcactttta gagggaacaa
    37501 ccgctgtctc tggctgttta tagctatatg ctgttttttt agcgaataca ggcgtttttt
    37561 taggcgtagg acgcatgcct tcgatttcca gtaactcctg atcgatttct tctacaaaac
    37621 gagaaggtcg gttatattgc gtccgtccat ataatgttct ggagaaagca tttgttaaat
    37681 acaacgcttc ttctgctcgc gtgatcccca catatgcgag acggcgttct tcctccagtt
    37741 cgctttcttc catcaatgct cgagataaag gaaaaacccc ctcttctaga ccaatcagga
    37801 atacaactgg aaattctaat cctttggctg catgcaaagt catcaatgtc acttgggaag
    37861 catcttcttc taaattgtca atgtcggaaa caagtgccag atcatttaag aagaccgtta
    37921 gtttttcttc tggtgcatca gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt
    37981 cttgtgtgac cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat
    38041 tttggatttt taagtcttct agatatccgc tacgatccaa aacctctttc gtcaattctg
    38101 tcactgtcaa gtatgggatc atctgagtga cttcttggat catctctcca aatgcaccta
    38161 attgctgtcc tgcttttcca ccgatatttg ccagatccac attttgcgct gcttcaagca
    38221 gtggccattc atgaagagaa gcaaaactgc gtagtttttc gatagatcct ggtccgatgc
    38281 ctcgtttggg cgaattaacg atacgctcaa aactgattga atcttgagga tttaccagca
    38341 cattcaaata cgctaaaata tctttgattt ctttacgatc gtagaacttg tgccctccaa
    38401 ccattttata aggaatattt gctttcaaca gagtttcttc cattacacga gattgtgcgt
    38461 tcgtccggta caagatggca aagtcgccgt aattccgatg attgcttcga atctcttctt
    38521 gcatccggtc tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga
    38581 tcttgtttcc ttctttgttt tctgtccaca agttttttgg ttttcgattg ctattgtttt
    38641 caatgacttg gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt tgttctagca
    38701 aaattactgc agcatctgga taatcttttt caaaatccaa gatgttctgc atatctgcac
    38761 cgcgccagcc gtaaatactt tgatctgcat ctcccacgac gcacaggttc ctaaatctac
    38821 ctgccaataa attgactagc gtatactgtg cgtgatttgt atcttggtac tcatctacat
    38881 gaatataatg gaatttgttt tgataatacg tgagtgaatc aggatgttct tcaaataatc
    38941 gaatcgtatt catgatcagg tcgtcaaagt ccatacattg attatattga agctcttttt
    39001 ggtaagcagc ataacatttt gccgcaatct cttcgtaata actgccttgc atttttgcaa
    39061 aatcctgagg ggtctgcagg ctgttttttg cttgagagat tgttcctaat atacttcgag
    39121 gatcatattt tttcggatca atgttcagtt cttttagtat ccgtttcatc aaggtcagct
    39181 gttcagaaga atcgatgatt gtaaagttcc ggttataccc gataaaatca acatctcttc
    39241 ttaaaatacg cacacacatc gagtgaaaag tcgataccca gacatcttcg cctccagatg
    39301 cgagaatcgc attgacacgt tctttcattt cttttgcagc cttattggta aaagtaatcg
    39361 ctaggatgtt ccaaggattg acttcttttt cctcaatcaa ataggcaatg cggtgtgtca
    39421 gcactctcgt ctttccactt cctgcacctg ccattagcag taatggtccg tcagtgtgaa
    39481 gaacagcttc tttctgacgg gggttcatcc cgtttaataa ttcagctttg gatgacatta
    39541 acaacatcct ttcttttatc aatcttgtta attatagcat tttcttggct ttcattaaac
    39601 tagctagttc aaaatcattt gtttcttttt atggattttt ccttgtcaat tcgttgattt
    39661 cgcgctaaat taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat
    39721 tagataagac tcagatatat tttattctaa aaggaatatt tgttggactg ctgacaggaa
    39781 tcgtcgttag cctgtttcga ttatctatcg aaaagctaag cgatttagtt cgaagtatct
    39841 atgaaatgag tcgggaacag ccgatttact taattggcct agctatttgc tgcatccttg
    39901 cagcattttt cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga agcgggattc
    39961 cacaagtaga gggacagctt cgcggggaat tatcaatgaa ctggttctct gttttatgga
    40021 aaaagttcat tggcggtgta ctttcagttg gtgcgggcct ttttctaggt agagaaggac
    40081 cttctataca gcttggcgca tccattggac aaggagctgg acaattgttt cggagtccat
    40141 cttcagaaga aaagatttta atttctagcg gtgcaagtag cggacttgca gcagcattca
    40201 atgctcctat cgcaggatta ctctttgttt tagaagaaat ccatcactcc ttttctccac
    40261 ttgtttggct gacatcattt gcttcagcaa tcactgctaa cttcgtctct ctttactttt
    40321 tcggcttgag acctgtttta tatctaggtg accttccttc tttgccgttg aaatattatg
    40381 gttccttagt gatcttagga gtacttctag gcatcttagg ttttatttat cagaatgtct
    40441 tgctggcttt gccaaaattt tacaaccgat tgcctttacc agcttatttt cacgggttcg
    40501 ttcctttctt gctgattttg ccaattggtt atttatggcc tagtactcta ggcggaggca
    40561 atcaaatcgt tttagctttt ggacatgctt ccttgccaat cattatgatt atcggtttgt
    40621 ttgttcttcg attcgttttt tccatgattt cttacggtgg gggtcttcca ggggggattt
    40681 tccttccgat attgacattg ggggctttaa tcggtagctt ttatggaaac attatggttg
    40741 atttcgggat ggatccaatc tacatcaagg attttgtcgt tattgccatg gctggttatt
    40801 tcacagcgat tggaaaagca ccgctgacag caattatttt agtgacagaa atggttggct
    40861 ctcttaatca tctgatgcct ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc
    40921 ttctcggcgg taatccaatt tacgaatcat tattggaacg tctacttgga aatactcaga
    40981 ccgcctcact aaaaggtgca aggacaactt ttgattttcc agtaacggca gagagttctt
    41041 tagacggatt aatggtacga gacttcaatt ggcctaaaaa tatgctgcta atctctatac
    41101 gcagaggaaa tcaggaaatc ttgactcacg gtgatacgat catgaatgta ggagatattt
    41161 tggttatctt gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat
    41221 cttctatcac aaatttgaag caatcatttt aacgaacatg gaaaaactat tttacaacaa
    41281 ctattcatct acactacttg attctatatg attctataaa aaaagaagag gctgggaaaa
    41341 cccagcctct tcttttttat ttctatgctt ctgctactgc aggagctttt ctcaataaac
    41401 cataaatcac accagatacg actgctccga tagcaataaa caccaaatac atgaccgggt
    41461 gactcagtaa gaatacaacg aaaatcccgc cgtgaggagc cagtaatttg attccgaaag
    41521 ctccgactaa tccgccagtt aaagcagaac caataataaa acttggaatg gcacgaatcg
    41581 ggtcagctgc tgcaaatgga atcgatcctt ccgtgacaaa cgatagtccc atcacgatgt
    41641 tcgtcaaacc agcatcttga tctgttttgc tgaatttatt cttaaacaaa cgtgtagcaa
    41701 cgaaaattgc caatggaggt accattcctc cagccataac agaagccatc acgacacttc
    41761 cgccagttgc aacagtcgta gctaaagttg ctgtaccaaa cacataagcg gctttgttga
    41821 ttggaccgcc taaatctgcc gccatcattc cagctaataa tgcaccaagt aaagcagcat
    41881 tcgaaccact catacttgtc aagaaaccgt tcaatgcatc attgattgct ttcataggga
    41941 cattgaccag caacatcaag aaaccagtaa tcagtaagcc aaacactgga taaaagagga
    42001 tcgttttaat tccatccaat gattttggta agttcttgaa taatttacgt aaaaatacga
    42061 tgacataacc agctaagaat ccaccaatca gtgcacctaa gaatcctgca ccgcctgtat
    42121 tagctaatgc accagcagca aatccaacga tcaaaccagg gcggtcaccg atacttgaag
    42181 caataaatcc tgctaatacc ggcagcatga aaccaaatgc agcttgtccg atttggttga
    42241 accagctagc agcttgattg taattcccta gatttgataa ttgatcatgt ggcacgccaa
    42301 taaattggtc gatcatgaaa gaaagcgcaa tagcgattcc tccaccaata acgaaaggta
    42361 acatgtgtga tacgccgttc atcaaatctt tgtaaattct ttgtccgata gtaccatccg
    42421 catttccttc ttctgaactg ctttcttttc catcaccatg gaatactggt gcagtaccac
    42481 taagggctag attgatcaac tcttctggtt tacggatacc atcactgaca ggacgattga
    42541 tcagttcttt tccgtcaaag cgattcatct ctactttttt gtctgctgca acgattacac
    42601 cttctgcacg ggcgatgtct tcagcagtca atcggttttt gattccttct gaaccgttcg
    42661 tttcgacttt gatctccaca cccatttctt tcgctttttt cttcaaagca tcttctgcca
    42721 tgtaagtgtg agcgatccct gttggacaag ctgtaacggc aacaacgaat tttctttctg
    42781 gatgagcaga agcttctgca gcttgtgttt cttgttctgc tttttcttct gcttcttttt
    42841 gctcttcagc tgtttggaac aagtcttgga cttcttctgg tgtatttgct tcttttaatt
    42901 ttcctacgaa atctgcatca atcagcaatc gagatagtgc tgctaatgct tgcaaatgtg
    42961 tgtcgtttgc gccttctgga gctgcgatca tgaaaaacag ataagttggc tgtccgtcta
    43021 aggcttcata atctactcct ttttggcttt ttgcgaataa aactgttgct tctttaactg
    43081 cactgttttt cgcgtgaggc atggcgattc catcaccaag acctgtagaa gtttgtgctt
    43141 cgcgggctag aattccttct ttatatgttt cgatatcgga aatccggccg gcatcaaaca
    43201 ttttttggac catttcatcg attgcgcctt ttttatcagt cgcttgcaaa tccatgatca
    43261 tcgcttcttt gaccattaaa tccttgatat tcatgattat cctccttgaa actaatcaat
    43321 ttttgtgatt tcaacttctg gcagcaactc atcaatcaat tcgcgagctg ccaagtcatc
    43381 agaaaaagct gttgcgcttc cgcaagcaac tccccattta aaggcttcga ttggatcttt
    43441 cgtacggctg taagccccaa taaagccagc aatcattgaa tcccctgcac ctactgaatt
    43501 tttcaatggg cgaactaata cattcgtttt ataagttcct tgttccgtga agagcaaagc
    43561 accgtctcca gccattgaga tcagcacatt ctgtgcccct tcagctagta attttttccc
    43621 atacggaatg atttcttcta cagattggaa ggttgtgttg taaagctcag ctaattcgtg
    43681 attgtttggt ttgatcaaca acggtttctt aacaagtgca ttttttaggt catcacctgt
    43741 tgtatcaatc acgaaatctg cacctttttc tcgaatgata tcaatcagtt cttgataaaa
    43801 tccttttcgc aggctagctg gcgcgcttcc tgacaatacg acgatatctc cttgtcctac
    43861 tgttcctaat gcttgcttca actcatcgat ttcttcatct gaaatagatg ggcctaagcc
    43921 attgatctca gtttcagtgt ctgattttag tttgatattg attcttgtat cttcttttac
    43981 ggttgtgaac aaagactgga tttgttcttt ttcaagccac ttcgaaataa attcgccagt
    44041 gaaccctcct aaaaacccta gtgctgtaga ttgagaatcg atccgtttca aaatacggga
    44101 aacattgatc ccttttcctc caggtaactt gaagtcttcc ttcattcggt tcaaatcgcc
    44161 tagttttaac ccatctacgc gtacgatata atcaattgat ggattaagtg ttactgtgta
    44221 aatcattgta ttgcctcctt aattgttgtc ttttcacgga attcattaac gaattctaat
    44281 ggacaacgct cggttatgat cgtggctgct tccaaaggtg caacagaggt gaaggacaac
    44341 tggttgaatt tggtatgatc aatcaataca aatgtctctt cggaattttt catggctaaa
    44401 cgtttaacag ctgcctcttc tggatccgga gtcgtgaatc cttgttcaag atgggcaccg
    44461 ttcattccca taaatgcttt attaaaatga aaatttttta gttgttctat cgcattagat
    44521 cctaaaacag cattagttgc cagctttatc gttccaccta aaataatggt agcaagctcc
    44581 aaatcaacta aatttgccgc atgttgaacg gaatttgtca ctactgtgat ctgttttcct
    44641 tgcagaaaag gaatcatttc taacgtagta gagccagcgt ctaaataaat gacatcttcg
    44701 tcattaatgg tttcagctgc cgatttggcg atttgctgtt tttcgtgaac gtttttgagt
    44761 gatttttcac tcatattctg ctcaaaattc aataattgct ctttcttagc tccgccatga
    44821 actctttcta gaaacccact attctccagc tcttgtaaat cacgtcttat cgttgattca
    44881 gaagcattca aaaggtttac aagatcttgt gatttcacga ttttctgctg ttcaagcaag
    44941 tctaggattt tttgtctgcg ttcctctgta agcattttca cgatcacctc aatacaaata
    45001 atagcatatt ttttttctac tagcaatata aatcagtcaa aaacgttcat aaaagttata
    45061 aatcagtcaa aaaaaaataa aaaaccagtc agaaagactg gttttttatt tttttgtcta
    45121 tcatcaagcc gttttatcgt tttttatttt cgcttcttgt ttttttcata taaacagaag
    45181 caggttttct ttccttatga agaagataat aataaagtcc catcccgcca aacatgacca
    45241 aaaaaccgat gactgatcga ataggaaaat taggaacaaa aaagagacag acggataata
    45301 atgatgccgg aaccattatt cgataataga gttgtttatc cattttcttc acctcatatt
    45361 ttttgtaaag taatcaattg ttctaattct cggtaatcta aaaattgcac acgcagatgc
    45421 tgctcacctt tcttgacatc gaattggata cttcgccgct ttttctttat tcgtttgaag
    45481 gtaacatcag agagtctatg tttgatttcc tttaacggta attctgacag ggattctgaa
    45541 ggaacataga ttcttaagga taaaagatac tcttgatatt tttcatctgt tacctctact
    45601 tctagatatt ccctaatata ataacaataa gcttcgattc cattatccaa agcaacagtc
    45661 ggatcgataa ctttatagca catcatgcgt gatcgattct cgtcaaccca aggaaaatac
    45721 gcctgagcat ttagtgttcc gcgaacacgc ctttctttat aatctccttg ttggatatca
    45781 taactttttg ttacaaaaaa tgaagcattc tttttaggaa caggttgcat ttgtgcattc
    45841 tgatactgga cagattggat cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc
    45901 aataaaccga caccattgtc ccaagcaaga tgatcaaacc caagctggtc cctaaaatag
    45961 gattgaaaca tttgattgtt tggcttttgc cagttaaagg ggtctcgata aagctggata
    46021 catatgactt cgttttctaa ttccttcact ttttctactt gttggatgtc gagaaaaatc
    46081 ggtttaggga tgatttgatg ataatagcga ctataataca tttcccaaca cgaagtgaaa
    46141 cataaacctt ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac tactacttcc
    46201 ccgttttgtt gtttcatttt aggtaaatgt tcgatttcat cctttttctc aaacaatagc
    46261 cgttgagaga tctgttttgt attgtgatag ataaactcgc tataagctcg catataggcg
    46321 aacaccccat gagcaatcat ttttttctga atgaagtctc gaatcaaaaa ttgcttttct
    46381 ttgaaaacag agatagacaa tacatttttt atcaataatt gattatcttc caagcgaaaa
    46441 ctacattcgt tctcgtcatt tcctacaact aaccaacttt cgtctcttac cgatgctagc
    46501 attatttttt cttgaagctt tcgaagatca tacggatccg cttgttgtgt gaaagagcgg
    46561 ataaatgttg gctggatcaa cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt
    46621 ttttcttctt catttaagtc tgtgagctgg atagtcatca ataatttatc ttcgccaatc
    46681 attttctcac ctctttcaaa tctttttata atttaattat aaccaatgat aaagaaaaac
    46741 taaaacttta tgtcttagaa accaatttgg acgattacat gatagactaa gtaaaaagaa
    46801 acaaagaggt gtttacaatg gggttgaaat ttgaacgtac agataattta gataaacttt
    46861 tcgaatcatt tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat
    46921 ttctaaaacc acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc
    46981 tcaaaaaaag acttaccagc caaactttta tgaaagagtt tgactggtaa gtctttttta
    47041 ttctttttat gattcaattg cttgttcgat tgctgcccac gcttcttctt ttccctgttt
    47101 tgtaacggaa gaaaagagaa taaacgtgtc attgcgatca aaatccaacg cttttttgat
    47161 ttcgctctca tgcttgttcc atttaccacg agggatctta tctgccttag tggcaacaac
    47221 aattacaggg atatcataat acttcaaaaa ttgatacatc tggatatctt cttgagaagg
    47281 cttatgacgc aaatctacta aagaaacgac agcacgtagc tggtctctag tcgttaaata
    47341 tgtctcgatc atctttcccc attttgctcg ctcagttttg gatacttttg catacccata
    47401 tcccggaaca tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt
    47461 ctttcctggc ttaccggaag tacgggctaa attctttcga tcaatcaatg tgttgataaa
    47521 agaagatttc cctacatttg atcgtcctgc cagagctatt tctggcaatt gtgtatctgg
    47581 atattgtttt ggtgccactg cacttatgat aatttcagct tgatggactt tcatggtctc
    47641 tctccttaac aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa atgactttta
    47701 cggcagcctc ttttcatcaa cccgcttttt tctctgaatc tttatagatt actgttggct
    47761 caccagacaa ttcagctgct tcttttgtga taatcacttt ttcgatgttt tcatttgaag
    47821 ggatatcaaa catcacatcc atcatgattt cttcaatgat ggaacgaagt cctcgagcac
    47881 cggtattgcg ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa
    47941 gttcggtatt atctagagaa agaagttttt gatattgttt gactagcgca tttttcggct
    48001 cagtcaagat ccgtaccaga tcatctgttg tcaatttctc taaagctgcc ataacaggta
    48061 atcgtccgat aaactcaggg atcaatccga attttagtaa atcttcagga atgatatgct
    48121 gcatgacact ttcgtcttca gaaagttttt ggttttgtgt accaaatcca atcgtctttt
    48181 cgcccatacg attcttcaca atcgtttcga tgccatcaaa tgcaccacct acgatgaaca
    48241 atatgttcgt cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt ccgccctgag
    48301 gtggcacact tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc
    48361 cggaaacgtc acgagtaata gatacatttt cgcttttacg cgcaatttta tcgatctcat
    48421 caatatagat gatccctttt tctgcaagtt ccacattgta atcagcagat tgaagcaatt
    48481 taagcaaaat gttttctaca tcttccccca cataaccagc ttctgtcaaa cttgtcgcat
    48541 ctgcgattgc aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac
    48601 cagaccctgt aggtccgatc aagcagatat tgcttttttg cagttcaaca tcatctgtgt
    48661 tttcttgtgc ttgctgattg acacgtttat agtgattgta tacggcaaca gctaacgagc
    48721 gttttgcgcg atcttgtcca atcacgtagt tattcaatac ttcaagaatt tctagtggtt
    48781 tagggacttc agtgaattcg cgtacagctt cttcataaaa ttcttcatca atgatttctt
    48841 tacataggtc gatacattca ttacagatgt agacccctgg accagctacg atctttttca
    48901 cttcttcttg ggttttcccg caaaaagaac agcgtacggt ctcattgcta cttggattgt
    48961 catacatggt cttcacccct taacttgcaa aaagtatgca agaaactatc aaagacttta
    49021 cagtctttta ctatcataac atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa
    49081 ctgacctata cctttgcatt ttttactctt aacaaaaaat aaggaactgt gacaaaatcg
    49141 tgtcacagtc ccttatttcg aataaacaat tttctagaag tagcttcttc agaaatgatt
    49201 tattacttga tttctattct ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt
    49261 ttttcattgt cacatcatgt tccagcatat cttctgtcaa cacgcgtttg atttgttcga
    49321 ttggcatgtt gtatgtttct gataattctt ggatttctgc attgatttct tcttctgttg
    49381 cttgaagatt ttcagcagct gcaattgctt cgatcacaag atttgttctt gtgcgcattt
    49441 ctgcttcacc ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt aattgatagt
    49501 acatttcagg agagatacct tgacgttgca tgttgttcaa gaattcgtcc attgaacgat
    49561 gtacttcatc atgaaccatc acgtgtggca attcaacgat ttctgcgttt tcaacagcca
    49621 tacggatcgc tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt
    49681 ctttgcgata tttttctttc aattcgtcta atgttgatac ttcgtcatca acatcttttg
    49741 cgaattcgtc atctaattca ggcacttctt ttgcttttac ttcatggatt gtcactttga
    49801 agactgcttc ttttccagca agatcttctg cttgataatc ttctgggaaa gttacattta
    49861 catctagact ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg
    49921 agcctgaacc taattcaagt gagtaatttt cgccttttcc gccatcaaac gcttcgccat
    49981 ctacaaagcc ttcgaagtcg attactactg tatcgccatt ttcagctgca gcatcttctt
    50041 tgataactaa ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat
    50101 ctgttacttc acggtcttgt ttttcaacaa ctagatcttt atattgtcct aattttactt
    50161 ctggtttcac tgttacttca gcagtgatta cccagtcttg acctttttcc atgctttcaa
    50221 catcgatttt tggttgagaa acaggatcga tgccagcttc tttgacagca gcttcataag
    50281 cttctggtaa tacagcattc aatgcatctt catataaagc ttcttcaccg tacatacggt
    50341 tgaatacttg acgagaaact ttccctttac ggaatccagg tacgttcaag ctttttttca
    50401 ctttattaaa cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta
    50461 aaacaccatc attcgtgcct tttttttcaa atgttgcagt catttaattc cctccgagtt
    50521 tagaaattca ttttatacat caaaaacgtt tcatgcatac ctttaaatag tacactaacc
    50581 ctttcaaatt gtaaacatat tgaatggata gaatccttgt ttttcataac atttttatct
    50641 ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt tctctttttt
    50701 ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct gtacacgcct
    50761 ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag acaggataaa
    50821 caattgcaga ttgcaacgtg aattcttctt ttaacacgga actaagcagc gcatcttcat
    50881 tcccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc tgcggctttg
    50941 gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag aaaacaggat
    51001 ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa atatggacct
    51061 ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga agctgttcga
    51121 tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc tccgatttat
    51181 tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct gcaaattgga
    51241 ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct atcaattttc
    51301 tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca gcaaactcct
    51361 cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct tttttctcgt
    51421 ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct ttcaactcgt
    51481 aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt gcttgttgtg
    51541 ccactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg cagtgtcctc
    51601 ctcttgctca tattcagctc atgttcaata gtagcaaaac aaaaaaagca tgacaattaa
    51661 aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga aggtgctttc
    51721 aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaac gctttgatca
    51781 gattgatcgt aacaacactt gtattagtga aaaaatcgcc cagatgttgc tttttctgat
    51841 taaagactgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg aaccggcaag
    51901 caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag ctgatgactc
    51961 gaataccaaa gatcattttc ccaatcgttt ggccatgatt gaatttcgtc aataaaataa
    52021 aatacgctag ataaataagt aaggctgtga atccgtagag actcagataa gaatctgaaa
    52081 aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata gctccgatac
    52141 acaaaagatc gaccaagaag gcaaacattc ggataaaaaa acctgcatag aaatacttag
    52201 gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc catttacgtt
    52261 gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc agtacctttt
    52321 gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt tcttgttcct
    52381 tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca ttgcttttgg
    52441 tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct cagaaggctt
    52501 caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc cagatggcgt
    52561 ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgta tagcttttaa
    52621 agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt ggacaccatc
    52681 ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc gtccctcgct
    52741 gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca ccgcttcttc
    52801 ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc ttttaactgt
    52861 tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg acatgatcac
    52921 accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt gagcagaaat
    52981 atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga tatcatgttc
    53041 tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac cgccaccagg
    53101 agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg ccttcagttg
    53161 ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag accctcccgc
    53221 agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc ccttttccaa
    53281 cacatttgga gctagttctt catccccata taaccaactg ttcaatccct caatttgatc
    53341 gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata atcctgcagc
    53401 aatcagaact gctgcccaac gtcttttatt cattctttct cctttctaat acaagttttt
    53461 tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt tttcttcccg
    53521 cgcatacccg gaaaacgact cttttgttaa ttcatcttct accgtaaact cacgagcttg
    53581 agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag tatctaaaaa
    53641 tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt gtttttttaa
    53701 cattcccagt gtcatgatct caatttttga atccattttt actcctcctc tatccgatta
    53761 ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta ttttttgaca
    53821 taaaaaggac atttgacaaa gaatttgata tataccaatt tattttttat tctttttttg
    53881 ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa aaacaaaaag
    53941 gaggatagtt atacctttta ggatagcgcc aggtctgtgt aaacagatga cgaggagagg
    54001 gtttatcgaa agattcggcg gatggcccta gggactgcac tctacagaaa atccacaaaa
    54061 aatgtctgca aagataaaac aaaaggattt tcctgcagct ggaagcattc atacgaattc
    54121 attatagaaa gtaggatgtt ttttatgtgc ggaattgttg gaatgattgg attaaaaaat
    54181 gtaacaccag gattgattga cggtctagaa aaattagaat accgtggtta tgattcagct
    54241 ggtatctttg tctcagatgg cacaactgat tatctggtca aagcacaagg cagaattcaa
    54301 aatttgaaaa acaaaataaa tattgatact acaggaaata taggtattgg tcatacacga
    54361 tgggcaactc acgggcaacc ttctgaagaa aacgcccatc cacatacttc ccaaagtgga
    54421 cgcttcgtgc tcgttcacaa cggcgtaatc gaaaactttg aagaacttaa aattgcttat
    54481 ttagcaaatg accactttat tggtgagacc gatactgaga ttatcgctca tctaattgaa
    54541 actttcgcga aggatactac aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa
    54601 ggttcttacg ctttcgcttt gattgatcgg acagcacctg atgttattta cgtcgctaaa
    54661 aataaaagtc cactattaat cggactcggc gatggattca atgtgattgc cagcgatgca
    54721 atggctatgc ttgctcatac gaaggaattc gttgagattg aagatgaaga gatggttacc
    54781 gtcacatcag aaaaagttat tattcaaaat tttgcaggtg acatcgtaga acgttcttca
    54841 ttcgaagcac aagtagatgc ttcagatatc gagaaaggta cctatccttt ttatatgctg
    54901 aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa
    54961 gacaatcgtg tctctcttga tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc
    55021 tatatcgttg cttgcggaac tagttaccat gcggggttag caggtaaaca gactttggaa
    55081 aaattgacac agattcctgt tgaggttcat ttagccagtg aatttggcta taacacacct
    55141 cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa gtggcgaaac agcagacagc
    55201 cgccaagtgc tggtaaaaat caactgtctc ggctaccctt cacttacgat tacaaatgtt
    55261 gctggttcta ctctttcgcg ggaagcatcc tttacactgc tattgcatgc tggacccgaa
    55321 atcgcagtag catcaacgaa agcttatact gcacaaatag ctgtactaac tttattagcc
    55381 aaagctatcg gtgataaaaa agaatttgaa acttctcttg tattcgatat cactcatgaa
    55441 ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc aaaaagatta tttagaagag
    55501 ttggcagaag tatatctcag cgatacaaga aatgcctttt acattggacg tggtgctgac
    55561 tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa
    55621 ggttttgccg ctggcgaatt gaaacatgga acgattgctt tgatcgaaga aggaacacca
    55681 gtgatcggaa tcatcagtga agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa
    55741 gtcgaaagcc gtggcgctaa aacactagtt attgtctcag agggattaga aaaggaaagt
    55801 gaccagctgg tgctaccaac tgtccaccct tatctaacta ctctaaccac agtcgtaccg
    55861 actcaattac ttgcatatta cgcaacgcta ttacggggat tcgacgttga caaaccacgt
    55921 aatttagcga aatcagtgac agtagaataa taaaagaaca aggaccagtg tcagacatct
    55981 gacactggtc cttgttcttt tatttt
//
LOCUS       DACEND010000003        54239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEND010000003 DACEND010000000
VERSION     DACEND010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.003"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW746_000130"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW746_000130"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1077062.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW746_000131"
     CDS             complement(937..1518)
                     /locus_tag="IW746_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1077063.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW746_000132"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW746_000132"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1077064.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW746_000133"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW746_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1077065.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW746_000134"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW746_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1077066.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW746_000135"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW746_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1077067.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW746_000136"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW746_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1077068.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW746_000137"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW746_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1077069.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW746_000138"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW746_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1077070.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW746_000139"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW746_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1077071.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW746_000140"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW746_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1077072.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW746_000141"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW746_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1077073.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW746_000142"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW746_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1077074.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW746_000143"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW746_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1077075.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW746_000144"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW746_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1077076.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW746_000145"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW746_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1077077.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW746_000146"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW746_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1077078.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW746_000147"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW746_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1077079.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW746_000148"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW746_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1077080.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW746_000149"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW746_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1077081.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW746_000150"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW746_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1077082.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW746_000151"
     CDS             13972..14157
                     /locus_tag="IW746_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1077083.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW746_000152"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW746_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1077084.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW746_000153"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW746_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1077085.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW746_000154"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW746_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1077086.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW746_000155"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW746_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1077087.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW746_000156"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW746_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1077088.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW746_000157"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW746_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1077089.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW746_000158"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW746_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1077090.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW746_000159"
     CDS             18232..18879
                     /locus_tag="IW746_000159"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1077091.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW746_000160"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW746_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1077092.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW746_000161"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW746_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1077093.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW746_000162"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW746_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1077094.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW746_000163"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW746_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1077095.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW746_000164"
     CDS             20317..21255
                     /locus_tag="IW746_000164"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1077096.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW746_000165"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW746_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1077097.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW746_000166"
     CDS             21935..22774
                     /locus_tag="IW746_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1077098.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW746_000167"
     CDS             22750..23619
                     /locus_tag="IW746_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1077099.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW746_000168"
     CDS             23609..24406
                     /locus_tag="IW746_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1077100.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW746_000169"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW746_000169"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1077101.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IW746_000170"
     CDS             complement(25688..28357)
                     /locus_tag="IW746_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1077102.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IW746_000171"
     CDS             complement(28595..28783)
                     /locus_tag="IW746_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1077103.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IW746_000172"
     CDS             29053..29415
                     /locus_tag="IW746_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1077104.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IW746_000173"
     CDS             29467..31149
                     /locus_tag="IW746_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1077105.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IW746_000174"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IW746_000174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1077106.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IW746_000175"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IW746_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1077107.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IW746_000176"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IW746_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1077108.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IW746_000177"
     CDS             34984..35988
                     /locus_tag="IW746_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077109.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IW746_000178"
     CDS             35989..36933
                     /locus_tag="IW746_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077110.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IW746_000179"
     CDS             36933..37895
                     /locus_tag="IW746_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077111.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IW746_000180"
     CDS             37922..38836
                     /locus_tag="IW746_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077112.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IW746_000181"
     CDS             38862..40643
                     /locus_tag="IW746_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1077113.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IW746_000182"
     CDS             40991..41677
                     /locus_tag="IW746_000182"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1077114.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IW746_000183"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IW746_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1077115.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IW746_000184"
     CDS             45287..46096
                     /locus_tag="IW746_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1077116.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IW746_000185"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IW746_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1077117.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IW746_000186"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IW746_000186"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1077118.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IW746_000187"
     CDS             complement(49648..50100)
                     /locus_tag="IW746_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1077119.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IW746_000188"
     CDS             50250..51200
                     /locus_tag="IW746_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1077120.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IW746_000189"
     CDS             51193..52152
                     /locus_tag="IW746_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1077121.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IW746_000190"
     CDS             52149..52904
                     /locus_tag="IW746_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1077122.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IW746_000191"
     CDS             52927..53880
                     /locus_tag="IW746_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1077123.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17798 a         9829 c        11684 g        14928 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tccgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg cttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctg
//
LOCUS       DACEND010000004        49945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEND010000004 DACEND010000000
VERSION     DACEND010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.004"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..1282
                     /locus_tag="IW746_000192"
     CDS             62..1282
                     /locus_tag="IW746_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1077124.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1279..3999
                     /locus_tag="IW746_000193"
     CDS             1279..3999
                     /locus_tag="IW746_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077125.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3983..4927
                     /locus_tag="IW746_000194"
     CDS             3983..4927
                     /locus_tag="IW746_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1077126.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4996..6006)
                     /locus_tag="IW746_000195"
     CDS             complement(4996..6006)
                     /locus_tag="IW746_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1077127.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6186..6530)
                     /locus_tag="IW746_000196"
     CDS             complement(6186..6530)
                     /locus_tag="IW746_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1077128.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6523..6957)
                     /locus_tag="IW746_000197"
     CDS             complement(6523..6957)
                     /locus_tag="IW746_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1077129.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7095..7832
                     /locus_tag="IW746_000198"
     CDS             7095..7832
                     /locus_tag="IW746_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077130.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7825..9036
                     /locus_tag="IW746_000199"
     CDS             7825..9036
                     /locus_tag="IW746_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077131.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9243..10022
                     /locus_tag="IW746_000200"
     CDS             9243..10022
                     /locus_tag="IW746_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1077132.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10092..10754
                     /gene="trmB"
                     /locus_tag="IW746_000201"
     CDS             10092..10754
                     /gene="trmB"
                     /locus_tag="IW746_000201"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1077133.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10852..12606)
                     /gene="ade"
                     /locus_tag="IW746_000202"
     CDS             complement(10852..12606)
                     /gene="ade"
                     /locus_tag="IW746_000202"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1077134.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12738..13667
                     /gene="cpaF"
                     /locus_tag="IW746_000203"
     CDS             12738..13667
                     /gene="cpaF"
                     /locus_tag="IW746_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1077135.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13624..14673
                     /locus_tag="IW746_000204"
     CDS             13624..14673
                     /locus_tag="IW746_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1077136.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14670..14948
                     /locus_tag="IW746_000205"
     CDS             14670..14948
                     /locus_tag="IW746_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1077137.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14923..15393
                     /locus_tag="IW746_000206"
     CDS             14923..15393
                     /locus_tag="IW746_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1077138.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15410..15712
                     /locus_tag="IW746_000207"
     CDS             15410..15712
                     /locus_tag="IW746_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077139.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15729..16100
                     /locus_tag="IW746_000208"
     CDS             15729..16100
                     /locus_tag="IW746_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1077140.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16072..16431
                     /locus_tag="IW746_000209"
     CDS             16072..16431
                     /locus_tag="IW746_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077141.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16603..17610
                     /locus_tag="IW746_000210"
     CDS             16603..17610
                     /locus_tag="IW746_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1077142.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17635..18819
                     /locus_tag="IW746_000211"
     CDS             17635..18819
                     /locus_tag="IW746_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1077143.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19256..20839)
                     /locus_tag="IW746_000212"
     CDS             complement(19256..20839)
                     /locus_tag="IW746_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1077144.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEVTVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21417..21560)
                     /locus_tag="IW746_000213"
     CDS             complement(21417..21560)
                     /locus_tag="IW746_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1077145.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21783..22262)
                     /locus_tag="IW746_000214"
     CDS             complement(21783..22262)
                     /locus_tag="IW746_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1077146.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22335..22619)
                     /locus_tag="IW746_000215"
     CDS             complement(22335..22619)
                     /locus_tag="IW746_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077147.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22697..22891)
                     /gene="ssrS"
                     /locus_tag="IW746_000216"
     ncRNA           complement(22697..22891)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW746_000216"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22963..24249)
                     /locus_tag="IW746_000217"
     CDS             complement(22963..24249)
                     /locus_tag="IW746_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1077148.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24387..25058
                     /locus_tag="IW746_000218"
     CDS             24387..25058
                     /locus_tag="IW746_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1077149.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25024..25512
                     /locus_tag="IW746_000219"
     CDS             25024..25512
                     /locus_tag="IW746_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1077150.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25525..26472
                     /gene="prmA"
                     /locus_tag="IW746_000220"
     CDS             25525..26472
                     /gene="prmA"
                     /locus_tag="IW746_000220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1077151.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26473..27237
                     /locus_tag="IW746_000221"
     CDS             26473..27237
                     /locus_tag="IW746_000221"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1077152.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27470..29683
                     /locus_tag="IW746_000222"
     CDS             27470..29683
                     /locus_tag="IW746_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1077153.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29694..30140
                     /locus_tag="IW746_000223"
     CDS             29694..30140
                     /locus_tag="IW746_000223"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1077154.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30311..31570
                     /locus_tag="IW746_000224"
     CDS             30311..31570
                     /locus_tag="IW746_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1077155.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31659..32606)
                     /locus_tag="IW746_000225"
     CDS             complement(31659..32606)
                     /locus_tag="IW746_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1077156.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33000..34292
                     /locus_tag="IW746_000226"
     CDS             33000..34292
                     /locus_tag="IW746_000226"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1077157.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34307..36076
                     /gene="aspS"
                     /locus_tag="IW746_000227"
     CDS             34307..36076
                     /gene="aspS"
                     /locus_tag="IW746_000227"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1077158.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36185..37060
                     /locus_tag="IW746_000228"
     CDS             36185..37060
                     /locus_tag="IW746_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1077159.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37326..39209
                     /locus_tag="IW746_000229"
     CDS             37326..39209
                     /locus_tag="IW746_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1077160.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39284..39568)
                     /locus_tag="IW746_000230"
     CDS             complement(39284..39568)
                     /locus_tag="IW746_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1077161.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39558..40292)
                     /locus_tag="IW746_000231"
     CDS             complement(39558..40292)
                     /locus_tag="IW746_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1077162.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40421..41044
                     /locus_tag="IW746_000232"
     CDS             40421..41044
                     /locus_tag="IW746_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1077163.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41236..42357
                     /locus_tag="IW746_000233"
     CDS             41236..42357
                     /locus_tag="IW746_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1077164.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42364..45492
                     /locus_tag="IW746_000234"
     CDS             42364..45492
                     /locus_tag="IW746_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1077165.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45511..48705
                     /locus_tag="IW746_000235"
     CDS             45511..48705
                     /locus_tag="IW746_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1077166.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48851..49558)
                     /locus_tag="IW746_000236"
     CDS             complement(48851..49558)
                     /locus_tag="IW746_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1077167.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16286 a         8793 c        10420 g        14446 t
ORIGIN      
        1 ttattgatga aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga ggaaattttt
       61 aatggtgaaa tttatccatg cggcagattt acatatggat cggtcatttg aaggactgac
      121 aactcttgat aaaactgtac aggaaaaact attgaagacg aatctgaccg ttttatcaaa
      181 tatcattgaa caggcaataa taaataacgt tgattttgtt ttacttgcag gagataattt
      241 tcatcaaaat cgtccttcat taaaaataca gaaacatttt tcagaacaga tgaaacggtt
      301 agaaaagaac catatacctg tatttataat attcggaaat catgatttct atcaaaaaga
      361 gcggtattgg ttctcctttc ctaaaaatgt ccatttattt acatccgaaa cggtagagac
      421 aaaaaaaata acgataaaaa gtggagaaac tgtctctctc agtggattta gttatcgtca
      481 accgtggatt caaaaagaca aagtgatgga atttccttct agagaactca cagattacca
      541 tattgggttg taccacgggg aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca
      601 accctcaaag atgcaagaaa agggatacga ttattgggct ttagggcata tccatgtgcc
      661 aacggtctta aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag gtcatactaa
      721 aaaggaacaa gcttctacat ctattctact agttgaatta agtaaaggaa gttgccaaat
      781 acatccgatt aaagtagcag aggtctattg gaagacaaag gaaatatcat tgaggaaagc
      841 aagaacgaca aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg
      901 atttagttta attgagataa aattaaaaga ttatgaccat ctcaatacag atgttttgga
      961 acggatacaa tcaggagagc tattagacta tttgttagag gaattctcag ataggataaa
     1021 tgatttcttt atttggcgta tttctctcat agagaatact ttttatacga agaccccact
     1081 tgctgcttct gctaaattga tggaacagtt atttcaatac tatcagacac ctcaagattt
     1141 ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg gctagaatat tgagcgaact
     1201 gccggaatat caagaagaaa cacttgaaaa agccaagcag ctgcttaatc aagatttttt
     1261 atttgaggag gatcaggaat gaagatttta aggatcgaga ttgctgcttt tggaaaatgg
     1321 cgtcaaaaaa gttttgactt ttattcaggc aatcagctga tctatggggg aaacgaagca
     1381 ggaaaatcaa cgatttatca atttattcaa gcaatattat ttggtttccc ttcaaaaggc
     1441 cgtaaaaaaa aggattatac accaaaagac ggctcagcct atggcggcaa aatatggctt
     1501 aaacatcctg tatatggtga atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa
     1561 tcgaaggtgt ggctggggga tcaagtggga agtgatgagt tactagaaaa actgatcgca
     1621 cctttgacga aagagttatt ccagcaggta tttactttcc agcaagagca gttgatggaa
     1681 ctagatcatt tgagagaaaa agagctacat gatgcgctga tttctttagg catcacgggc
     1741 agttctcaag tattccaaaa gcggcaagaa tattatcaga aagcacaaca attattcaag
     1801 aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa
     1861 aaaattcatc aaaaacaaga acaggaagct tattttggca agttgatcca acaagaacag
     1921 gaatacgagt cgcaacagca gcagttattg gcacaattaa aagaggcgaa caaacaattt
     1981 tcattagcca gacagcaaga acttaatttt tctttatatg aagaatggca atctctcaaa
     2041 aaaaacagca ctacaggtct tcctgatgag caacttcttt tagatctgga gtctttctcc
     2101 aaacggtatc agcaaacaaa tgagcgtttg gaagaattag aagcaacact ggaaaagcat
     2161 agcgggatgg atcagcagtc agccagatat tatttttatt tagagcagga gcaggaacta
     2221 aaagatctcc agcaacttca atttacagcg gaacaattaa acgaagaatg gcaaagatta
     2281 caaattgaga ctagagaaac aaattcagaa ttgaagattt tggaacaaag gtggcattgg
     2341 cagaaaaatc cgttaccgga agaattttta tatgaagaag aatggaagca tcttctggct
     2401 gaagaaagaa ggattgctga tctagaaagc cagatttctg tgcgtctcca aatagctaaa
     2461 gaacagcaag agatgattga agcagaagtg aatgacttgg aagagaccta tccagcatta
     2521 ttacaaaatc agtcagagat agcaccagtc tcttcatcta aaccgtatat gctgatggct
     2581 ggcggagcaa taatcattgc aggtatattt gtaccaatgc ccttgaaagt gatcctttta
     2641 ctaattggtg caggcatgat tggctatttc ttctacaaaa ggcaaccaaa aattaaaact
     2701 aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa aactaggaca acttgatcta
     2761 gccttagctc aattagctga agcagaagaa aaacaaaaga gaactggaga agaaaaacag
     2821 attcttcagc aagaagtata tcgttttgcg gatcatcggc acttaggaaa gatggatacg
     2881 ctcaaactca tgaaagaata tggacaggaa gtcatagatt atcagaaatt atatggcgta
     2941 tttgagcgga aacataaaag agaacaggaa atacaagaaa aactacgaca aatcgaccaa
     3001 aaatttgagt ttgttaaaga atggttaccg atacaagata agacaatttc agaaaaaatg
     3061 gtattgcttt cccgttttat acaagagatg gaacagatga aatttgccag aagctatcag
     3121 caaaatactt tattgaagca agaaatcaat caattgaaaa agaaacaaaa agtatggata
     3181 caagaataca gcgaacaatt gactcaagca ggcttatctt atccttcgga agtacctgtc
     3241 tttttacaaa aagccaaaca agccgaacag cagatgaaaa gatacaatga attatcggaa
     3301 atgcttactc cactatttcc tgaagagctg acaaaagaga aattatctgt aagactgcgc
     3361 caactgaaag aaaagcaatc ccaactccag caagaagaaa aagaagcaag tgaaaatcgg
     3421 caacgactcc aacttcaagt agaagagttg caaagagacg gcacattgga cgaactttat
     3481 caacaagcta gccggcaaaa agcaaaatta caggaattac tggaggaatg gggagcactt
     3541 gaaatcgccg ggacattctt aggtgatctt tcaacagaga tgagtgaaag acaattaccc
     3601 cagttgttaa gagcagcagg tgattattta ggtattttaa caaaaggaaa ttatcagaag
     3661 atattactga aaaatgatac actgacttta acagacggat atacagaatt tcccatctat
     3721 gagttatcta caggtacgaa agatcagctg attatggcga tgagatttgc ttacctctca
     3781 ttagaagcta accgttcgat atgcccgatc atcattgatg atggctggct tcattatgat
     3841 catcagcgaa agtatcaatt agctgaactt ttagctaaat ttggagaaaa gtatcaaata
     3901 atttgttttt cttctgataa agaaatggta agctattacc atgagatgaa ccaaatcgta
     3961 aaacaattag aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt
     4021 tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt
     4081 tattgctttt actttccaag atacttcagg tactatcgat ggtaaatact gggatgcttc
     4141 ggaggaggaa atcaataaat ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga
     4201 ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa gggtagatga
     4261 gccaggagag cctagtttat acatggaaag agcccctttg aagaaagaag aaatggaaga
     4321 agaaatcaat caattgttgt ttgagatcac caatgcgcat tggaatcgta ttgttcgcta
     4381 tctacttaat aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa gaaaccatca
     4441 tgcatttgcc agtggattag cttatcatac tttgacaatg cttcacttag ggaaatcaat
     4501 cgccgatgaa tatcctgagc tgaatcgagc attgttgtat gctggtatca ttttgcatga
     4561 tttaggaaag gtgattgaac tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa
     4621 tttgatcggt catctggtat tgattgacga agaaataact aaagcctgtc ttgcgttgaa
     4681 aatcagcgaa gctgacgaag atgttctcgt attacgtcat atggttttag cacaccatgg
     4741 cttattagaa tatggctcac ctgtacgtcc aagaataatg gaagcagaaa tattacatca
     4801 aatcgacaat atcgatgctt cgatgcagat gatgttgact tcgattcgtc aaacagaacc
     4861 aggaaaatac accgatcgga ttttcggtat ggacaaccga agtttctatg taccaaaaga
     4921 tttatagcaa aaatttattt ttttcattca aaacaaaaaa agcgagacat aagtcttcgc
     4981 tttttttgtt ttcttttatt ttgatgaatc agttgtttct tcagttgaag attcttttgt
     5041 tgaatcagtt gattttgtgt cagaactctt tgttgaagct gtagtttcac ttgagtcttt
     5101 cgtacttgat gaagttgttg tgaatgcact caacacattt tcaaaggcat catctttgat
     5161 ttttacattt gcatctttta attcttcacc gattacttta gttgtgaatg cattgtcact
     5221 tagtttagtt tcagtagcga tatcttttag ttgatctttg tatttatcca tgtcattgcc
     5281 tttattttgg tttttcacca ttttgacaac gtagtattct gtcgcataag aagtaggatt
     5341 tgttgtagta ataacatcgg agatctcacc gtctttcaat ttgaaagctg cttcttttac
     5401 ttctgcagga atagttgttg tcgttgagtc gaatttaact ttaccgccat cttcttttgt
     5461 ctctgtatca gttgatttgt ctttggctaa tttagagaaa tcatctccat catccgctga
     5521 ttttttcaca tcttttgctt catcttcact tgataatttg atgatttgtg cttctacttc
     5581 tggatggaaa gatttccaag cagttttcaa atcgtcatct gtaatatcta cgtgagcttt
     5641 caagccagct tcaaaagcta aattgttacg aatatattct ttataagtat ctttcgtata
     5701 tccagcagct tctaattgtg attcaaaagt atcacctaat gatttcgctt gtttgtcgta
     5761 ttctgcatcc acttgcttat cggttacttt atcgccatat ttgttattga atactttata
     5821 aatgatcatc cgttgtacta aggattggtt cgatgattca agttttgctt catcatagaa
     5881 atcagataca gtgatcgttc cgcctttcat cgttgcgata tctttattag tatcgcctga
     5941 acaagctgct agagttaaaa cagccaacgc actagtagct gccaaaataa ttgatttttt
     6001 tttcattaaa atttgcactc ctgtaattag ttattatttt tatatattaa ctataccata
     6061 tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac
     6121 attcctagga aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat
     6181 cattattata tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca
     6241 ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt
     6301 gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt
     6361 ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct
     6421 tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca
     6481 gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac
     6541 ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg
     6601 attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg
     6661 tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt
     6721 catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc
     6781 tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa
     6841 tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata
     6901 tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata
     6961 aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat
     7021 tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg
     7081 gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc
     7141 ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac
     7201 tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa
     7261 aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac
     7321 aaattgggta tattccagag actccttctt tatatgaaga attgacattg agagaacata
     7381 tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa
     7441 agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa
     7501 agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata
     7561 tcatcgacga accattttta ggcttggatc cactagctat caatgcattg cttgaattga
     7621 tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg
     7681 cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt
     7741 ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata
     7801 ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat
     7861 caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt
     7921 cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca
     7981 ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt
     8041 aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa
     8101 atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg
     8161 atattggcat gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg
     8221 atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag
     8281 agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt
     8341 ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca
     8401 ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga
     8461 ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt
     8521 atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg
     8581 gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca
     8641 agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt
     8701 gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt
     8761 ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc
     8821 gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca
     8881 tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg
     8941 cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta
     9001 tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa
     9061 tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa
     9121 aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt
     9181 gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca
     9241 tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa
     9301 aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga
     9361 tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg
     9421 ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg
     9481 agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc
     9541 ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa
     9601 aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc
     9661 gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag
     9721 atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt
     9781 caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac
     9841 tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg
     9901 aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac
     9961 gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct
    10021 gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat
    10081 ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc
    10141 cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa
    10201 acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg
    10261 caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga
    10321 ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg
    10381 gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt
    10441 ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg
    10501 ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag
    10561 gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat
    10621 ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat
    10681 tttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa
    10741 agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat
    10801 cccagaaaat gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt
    10861 atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa
    10921 tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa
    10981 aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa
    11041 ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac
    11101 acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag
    11161 attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc
    11221 atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa
    11281 tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc
    11341 gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac
    11401 tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa
    11461 cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga
    11521 atgatcagca aataaatggt cttggagttg cccagattga acaactaatg ttcccttata
    11581 gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata
    11641 cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc
    11701 catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc
    11761 gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct
    11821 tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc
    11881 ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt
    11941 agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat
    12001 accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag
    12061 gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac
    12121 ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc
    12181 aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct
    12241 tgcatcttct atcataaact caagacctgt actcccagca atgtttccta tttcatgggg
    12301 atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga
    12361 taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc
    12421 attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca
    12481 gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt
    12541 gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt
    12601 ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt cggtttttgc
    12661 caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt
    12721 ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt
    12781 ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt
    12841 ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac
    12901 tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt
    12961 taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag
    13021 gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac
    13081 cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg
    13141 gattatacct tttcagcgga ccagtcggat caggcaagac ttctctaatg tatcggttgg
    13201 ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt
    13261 tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgagac
    13321 tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg
    13381 cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac
    13441 gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat
    13501 ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt
    13561 ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa
    13621 gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga
    13681 agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg
    13741 caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat
    13801 atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat
    13861 ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact
    13921 ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag
    13981 ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg
    14041 ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt
    14101 cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg
    14161 gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata
    14221 agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta
    14281 gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag
    14341 gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag
    14401 gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt
    14461 aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc
    14521 gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca
    14581 atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct
    14641 gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg
    14701 gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt
    14761 tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca
    14821 tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta
    14881 ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc
    14941 ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt
    15001 ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa
    15061 ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc
    15121 gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt
    15181 tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt
    15241 accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta
    15301 aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata
    15361 gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct
    15421 tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt
    15481 gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga
    15541 tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata
    15601 cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa
    15661 agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa
    15721 ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca
    15781 tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac
    15841 aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact
    15901 acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa
    15961 gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa
    16021 aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat
    16081 ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt
    16141 ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg
    16201 gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta
    16261 tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct
    16321 gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc
    16381 tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt
    16441 cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt
    16501 cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca
    16561 ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg
    16621 gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact
    16681 tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt
    16741 gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag
    16801 ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg
    16861 aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt
    16921 tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt
    16981 ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat
    17041 gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca
    17101 gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag
    17161 gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg
    17221 aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat
    17281 catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg
    17341 atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa
    17401 gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg
    17461 caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc
    17521 ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag
    17581 tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct
    17641 aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca
    17701 gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt
    17761 accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa
    17821 gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat
    17881 gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta
    17941 gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat
    18001 aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt
    18061 gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc
    18121 ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt
    18181 catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa
    18241 atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc
    18301 atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt
    18361 gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa
    18421 atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat
    18481 atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc
    18541 gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca
    18601 attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa
    18661 ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa
    18721 gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa
    18781 ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata
    18841 aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc
    18901 aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg
    18961 cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat
    19021 cccaatattt tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac
    19081 atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa
    19141 accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc
    19201 ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt
    19261 caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt
    19321 ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt
    19381 cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat
    19441 ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt
    19501 caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg
    19561 ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac
    19621 ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac
    19681 ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg
    19741 attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac
    19801 tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa
    19861 cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg
    19921 gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga
    19981 aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc
    20041 gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt
    20101 tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt
    20161 cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg
    20221 atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat
    20281 tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga
    20341 ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc
    20401 ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa
    20461 agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg
    20521 tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat
    20581 ggagcaagta gtggttgacg gtcacttcgg aaactgtaag ttcttgttcg accgtcccga
    20641 tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga
    20701 tttgtaaaaa ggttgatttg ccagttccgt tgataccgac taaggcaatt ttttcaccag
    20761 cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag
    20821 aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc
    20881 catggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg
    20941 attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc
    21001 aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt
    21061 catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag
    21121 tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc
    21181 agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga
    21241 gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac
    21301 ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca
    21361 ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa
    21421 ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa
    21481 aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca
    21541 gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt
    21601 aaaccacacc agggcgacta atcgccctgg tgttcacttt tttttatttt acttgaactc
    21661 attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt gccgcgtctt
    21721 ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg
    21781 tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt tcggacaaca
    21841 agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat aaataaacgt
    21901 tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt atcttctggg
    21961 atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt tacattattc
    22021 aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc catttcagta
    22081 gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg
    22141 acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc ctttttaaaa
    22201 gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta ttgttgtaac
    22261 ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa cactttaggg
    22321 aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg gatactgaag
    22381 gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg ttgattgaaa
    22441 aaagctgtta aaatacccat tacgcctaaa acagcatatg cagctccgta aaattgtagg
    22501 aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg tttattaaat
    22561 aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat cctaatcatg
    22621 aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag caaattgctt
    22681 tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag tattgatccc
    22741 gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg
    22801 ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg gtcaacacac
    22861 aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat gaatttaaaa
    22921 agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt ttggaggatg
    22981 ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat atttccctgt
    23041 atcttttggc tgataatact gagcactctt cagtttatct ggtaaatatt gctgattgac
    23101 ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga gtgatttcgc
    23161 acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc cctctcgaat
    23221 atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg acaagcaaag
    23281 atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt tttcagcagc
    23341 taatacagcg ttcactgtac gagccgcagc agcgggatta cctaaaccta tatcttcata
    23401 accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa ctaaacgggc
    23461 gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa aagccgaaat
    23521 aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt ggacacattc
    23581 ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag tcgatagtgt
    23641 agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg
    23701 aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt ctttatccct
    23761 taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga cttcaaagat
    23821 ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag tagtcgctcc
    23881 gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt ttgttttatc
    23941 taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg cttcttccgc
    24001 tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa atgcataatt
    24061 agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag gtccatatag
    24121 aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc cttccccgac
    24181 tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg
    24241 ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat catatttagc
    24301 aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc caactaatat
    24361 tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat gaattttttt
    24421 ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg gagcatgaca
    24481 caccagaagg aagattagct ggatatattg tagacgcgga agcataccta ggtccagaag
    24541 acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa
    24601 aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac atcattactc
    24661 agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg
    24721 atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa
    24781 aattagtgga agcattagca attcctcaag aactttatgg tcagtctata gcagatagtt
    24841 cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa
    24901 tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa
    24961 atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt tggaggaaag
    25021 aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa acaagtgaca
    25081 gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat cgttgttcgc
    25141 ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat cgtttcgcaa
    25201 gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa atctgttttt
    25261 gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca
    25321 gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca aagcgatttg
    25381 cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg ggataatcag
    25441 caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta tttaccgtac
    25501 ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga aactgaaagt
    25561 gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg tgtcgcgatt
    25621 gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat tttagataaa
    25681 aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc ggaaacgact
    25741 ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc tgagtttggt
    25801 ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca
    25861 gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc
    25921 tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga tcctggtatg
    25981 gcatttggca caggcactca tcccacgact tccttgacat tgacagcact agaaacagtc
    26041 ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct aagtattgca
    26101 gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt agccgttcgt
    26161 tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt atcagctaac
    26221 gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat tttagcagat
    26281 atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg cacgctgatc
    26341 gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt agaacaaggg
    26401 tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt gaaaaaacca
    26461 gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa agactatttt
    26521 gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga acccgaggat
    26581 gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac ggagattact
    26641 gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga actgccaagt
    26701 tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat cgttcagaaa
    26761 tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc tgtcgttaaa
    26821 tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc tcaagaggca
    26881 gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac gaaacaagat
    26941 ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga atcagcaaag
    27001 caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc
    27061 ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta ttttgtagaa
    27121 cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac agcacctctt
    27181 tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt cagctaacgt
    27241 tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac aatttgtata
    27301 atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc
    27361 gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat caaacagata
    27421 tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga
    27481 agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg gtcccgaaca
    27541 tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg gacagtttag
    27601 aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat tagcagattt
    27661 aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg aagatacaga
    27721 aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt tagtggacgg
    27781 cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag cagaaaacca
    27841 ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca aattagctga
    27901 tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc
    27961 tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca gccggatcaa
    28021 atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt accgtatcgt
    28081 ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga
    28141 tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac ggccaaaaca
    28201 catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg aaatttatga
    28261 cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg tattaggagc
    28321 aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg ccatgccaaa
    28381 agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa atccggtaga
    28441 aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg ctgcacactg
    28501 ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga aacaattaag
    28561 ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg aatttatgga
    28621 aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa aaggagacgt
    28681 tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc atacagatat
    28741 cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt
    28801 gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc caagtcgcga
    28861 ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc
    28921 ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga
    28981 tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag cactcgatcg
    29041 ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag aagttagccc
    29101 cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca
    29161 aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt
    29221 acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc gtatcagtcg
    29281 ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag gcagagggat
    29341 atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg
    29401 tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg atgcagattt
    29461 ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa cagtcaatgc
    29521 gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa tggcgacgat
    29581 ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg ataaaatcaa
    29641 agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca gatatgagag
    29701 cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa
    29761 ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg
    29821 actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa ggaaaaatga
    29881 atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc acactgtttg
    29941 cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca gaaacagcta
    30001 ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg
    30061 gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta acgatcatta
    30121 ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat gaaacacgtc
    30181 gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa aaagcgcttc
    30241 ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt tttataggag
    30301 gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt taaaaaaatt
    30361 gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg ctcccgtcgt
    30421 caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag cagcaaaagt
    30481 attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag tattgaagac
    30541 actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc atcggaaaaa
    30601 tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta tcttagataa
    30661 gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg atttaagtaa
    30721 gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag cgatttctaa
    30781 agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa cgtttacttc
    30841 tccgattgat cacataaaag gtgtcgaaaa tcgcaaaaaa tcagaaaaat gggcgcaagg
    30901 cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc
    30961 ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg cagctgttga
    31021 attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat tgatgggaca
    31081 tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt cgacgttaca
    31141 agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc tacctgcgat
    31201 ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt gggttatttt
    31261 agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt cctttatgat
    31321 caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg agatgattgc
    31381 acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga tcactcttca
    31441 tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg tacctgaaca
    31501 agattggacg attccattca atcagtttcc gacttatccg aatagccgag cgctacttta
    31561 caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt gctgatcttc
    31621 cttttttcat attactataa attgataagg tcaattgatc aaaatccacg aaatgcttca
    31681 tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac attgtcagat
    31741 acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt cttcatgtac
    31801 caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg gcgaagtaca
    31861 tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga aatctggatt tctttttcga
    31921 ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt cagcaaaccg
    31981 gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata agggaataat
    32041 tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc gacatatcca
    32101 tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac tgctcggatt
    32161 tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg tgtgctttca
    32221 tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt tagtgcatct
    32281 tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca
    32341 tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc cttcagtacc
    32401 tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc gatcaaattg
    32461 atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc caaaccgtca
    32521 aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc gtagacttgt
    32581 gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag tctaaaacaa
    32641 aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat atctcttgac
    32701 tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa
    32761 gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa gtttcattag
    32821 cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt
    32881 aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc attcaccctt
    32941 gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga gagaaaacta
    33001 tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca gaaaaatggc
    33061 agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat gaaatgcgta
    33121 caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca acggatatcg
    33181 tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg cttcgaccag
    33241 aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc ccagagtatg
    33301 cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc cctcaaaaag
    33361 gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa aaccctgcaa
    33421 ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg atcacgcaaa
    33481 tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat
    33541 tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa cgccgccttc
    33601 atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa tttgtggcag
    33661 aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt gataccgttg
    33721 tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg gttcgcggac
    33781 tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa atgggggcac
    33841 aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta ggtggtcctt
    33901 caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact atggaggcag
    33961 aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca ctcggagacg
    34021 aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt ttttcagctg
    34081 accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg
    34141 ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag
    34201 caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa cattttgatg
    34261 aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg cacaaagaac
    34321 gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat taaaaggatg
    34381 ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg atcgtgaagg
    34441 aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa tcgcggataa
    34501 atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc
    34561 agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt
    34621 gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca gtgacgaaat
    34681 acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt
    34741 aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact ttctcgatat
    34801 cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc tagttccttc
    34861 acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt
    34921 gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga
    34981 tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa cgttcttaac
    35041 agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga aagaagttcg
    35101 cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa tggctcgcta
    35161 cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa gcgatacagt
    35221 caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc
    35281 attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc agttaggtca
    35341 atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag aagacggatt
    35401 gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta aagcaacaga
    35461 tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc
    35521 attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg aatcgaaatt
    35581 caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag aaggtcgata
    35641 tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt tattggctga
    35701 agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt atgaactagg
    35761 cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag aaactctagg
    35821 ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt tagattacgg
    35881 attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt tattagccgg
    35941 agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat
    36001 gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata ttgatgtaac
    36061 gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg cttcttttga
    36121 caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa agcggggctt
    36181 ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca agctttcggt
    36241 atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc agccaggaca
    36301 tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt caaaatcact
    36361 gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac ctttgtttgt
    36421 attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca cagtcacttt
    36481 gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc caatcatctg
    36541 tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga gaaacggcct
    36601 atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat
    36661 tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa ttctatttgg
    36721 ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag taactgatgt
    36781 cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc cagaaaaagt
    36841 agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg aagcagaagg
    36901 tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat acgaactgcc
    36961 tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta ttgcagaaaa
    37021 cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg acaaaaattc
    37081 atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac catagtagaa
    37141 tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa aaagcatgtt
    37201 tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca cgattctact
    37261 tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat
    37321 attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc
    37381 tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag
    37441 gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc
    37501 tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa
    37561 tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt
    37621 tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg
    37681 tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt
    37741 caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga
    37801 tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt
    37861 taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc
    37921 caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc
    37981 ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata
    38041 ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag
    38101 atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag
    38161 tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag
    38221 tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga
    38281 ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg
    38341 aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag
    38401 cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca
    38461 tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg
    38521 gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa
    38581 tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt
    38641 cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac
    38701 aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac
    38761 aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg
    38821 taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgatcggac
    38881 taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg
    38941 atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga
    39001 atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca
    39061 gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag
    39121 ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga
    39181 acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt
    39241 gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa
    39301 gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga
    39361 atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct
    39421 taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct
    39481 ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata
    39541 atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct
    39601 ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct
    39661 ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg
    39721 atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca
    39781 ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc
    39841 acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc
    39901 gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa
    39961 ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca
    40021 tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg
    40081 atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct
    40141 gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca
    40201 gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat
    40261 aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt
    40321 cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt
    40381 ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg
    40441 cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga
    40501 agttttaccg caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc
    40561 attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact
    40621 gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag
    40681 agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt
    40741 gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc
    40801 attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac
    40861 acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt
    40921 atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc
    40981 agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa
    41041 ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga
    41101 ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg
    41161 taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt
    41221 ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta
    41281 caaggttatg acttgattga agaccagcgt catgtactat cttctatact agatatcgcg
    41341 aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca
    41401 gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag
    41461 atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg
    41521 ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca
    41581 gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga
    41641 ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat
    41701 aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta
    41761 atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc
    41821 cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct
    41881 gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag
    41941 atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt
    42001 agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg
    42061 gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat
    42121 agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg
    42181 attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg
    42241 aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact
    42301 gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca
    42361 agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta
    42421 gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc
    42481 aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt
    42541 cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact
    42601 ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta
    42661 gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt
    42721 gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc
    42781 agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga
    42841 gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta
    42901 ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag
    42961 caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta
    43021 gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat
    43081 caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa
    43141 aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa
    43201 gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag
    43261 gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag
    43321 cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca
    43381 aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc
    43441 gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg
    43501 gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca
    43561 ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa
    43621 agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt
    43681 gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca
    43741 aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata
    43801 acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa
    43861 tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt
    43921 gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa
    43981 gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag
    44041 caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta
    44101 gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag
    44161 gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta
    44221 atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa
    44281 gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag
    44341 acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa
    44401 ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta
    44461 gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat
    44521 cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa aatagatgaa
    44581 aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca
    44641 acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt
    44701 acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa
    44761 aatatccagc taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa
    44821 ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt
    44881 agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta
    44941 gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag
    45001 actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat
    45061 gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc
    45121 agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct
    45181 cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta
    45241 gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt
    45301 gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa
    45361 acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa
    45421 cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat
    45481 caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt
    45541 gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa
    45601 gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt
    45661 acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag
    45721 catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa
    45781 gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt
    45841 ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac
    45901 caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac
    45961 ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat
    46021 tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt
    46081 ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt
    46141 gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa
    46201 agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact
    46261 acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa
    46321 aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg
    46381 aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg gacattttat
    46441 tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg
    46501 gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc
    46561 cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca
    46621 gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga
    46681 gtcgatttcg tctatggcga agtcatctat tcgactgacc cgaagcatga ggtctcgaca
    46741 gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc
    46801 agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa
    46861 gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga
    46921 cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa
    46981 gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa
    47041 gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat
    47101 ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa
    47161 ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg
    47221 atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg
    47281 gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca
    47341 acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag
    47401 tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt
    47461 ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc
    47521 ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta
    47581 atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg
    47641 acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat
    47701 ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg
    47761 agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta
    47821 gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa
    47881 ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga
    47941 gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca
    48001 cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc
    48061 acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca
    48121 agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc
    48181 ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt
    48241 ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta
    48301 gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta
    48361 gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact
    48421 ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc
    48481 attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg
    48541 tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa
    48601 cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa
    48661 gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc
    48721 gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat
    48781 agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta
    48841 tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa
    48901 tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg
    48961 cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata
    49021 aggtctcttc tggaagcata tgaaattctt tctcagctag ctgaaaaatc tctttttctg
    49081 gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca
    49141 attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta
    49201 atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga
    49261 tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat
    49321 gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg
    49381 tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat
    49441 atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa
    49501 atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa
    49561 ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa
    49621 aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga
    49681 cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag
    49741 tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa tgcaatctct
    49801 gtggaatttt cgaaagattt atttttttaa tacagtctca atataactat tcaaacataa
    49861 aataacgtta tttgatttat ttgtttatag taaattttat ttagtaatca ttgctttaaa
    49921 atactacgct tatataacag agaaa
//
LOCUS       DACEND010000005        49885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEND010000005 DACEND010000000
VERSION     DACEND010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.005"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW746_000237"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW746_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1077168.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW746_000238"
     CDS             complement(322..891)
                     /locus_tag="IW746_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1077169.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW746_000239"
     CDS             1077..1979
                     /locus_tag="IW746_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1077170.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW746_000240"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW746_000240"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1077171.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW746_000241"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW746_000241"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1077172.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW746_000242"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW746_000242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1077173.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IW746_000243"
     CDS             8547..8732
                     /locus_tag="IW746_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1077174.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IW746_000244"
     CDS             complement(8794..9990)
                     /locus_tag="IW746_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1077175.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPLMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IW746_000245"
     CDS             complement(10160..11596)
                     /locus_tag="IW746_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1077176.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IW746_000246"
     CDS             11889..12698
                     /locus_tag="IW746_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1077177.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IW746_000247"
     CDS             complement(12884..15037)
                     /locus_tag="IW746_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1077178.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15331..16179)
                     /locus_tag="IW746_000248"
     CDS             complement(15331..16179)
                     /locus_tag="IW746_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1077179.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IW746_000249"
     CDS             complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IW746_000249"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1077180.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18680..18859)
                     /locus_tag="IW746_000250"
     CDS             complement(18680..18859)
                     /locus_tag="IW746_000250"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1077181.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IW746_000251"
     CDS             complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IW746_000251"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1077182.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21007..23286
                     /locus_tag="IW746_000252"
     CDS             21007..23286
                     /locus_tag="IW746_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077183.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23379..24026)
                     /locus_tag="IW746_000253"
     CDS             complement(23379..24026)
                     /locus_tag="IW746_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077184.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24205..24924)
                     /locus_tag="IW746_000254"
     CDS             complement(24205..24924)
                     /locus_tag="IW746_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1077185.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25055..26206)
                     /locus_tag="IW746_000255"
     CDS             complement(25055..26206)
                     /locus_tag="IW746_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1077186.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26423..27163)
                     /locus_tag="IW746_000256"
     CDS             complement(26423..27163)
                     /locus_tag="IW746_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1077187.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IW746_000257"
     CDS             complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IW746_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1077188.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27547..28140)
                     /locus_tag="IW746_000258"
     CDS             complement(27547..28140)
                     /locus_tag="IW746_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1077189.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28124..28771)
                     /locus_tag="IW746_000259"
     CDS             complement(28124..28771)
                     /locus_tag="IW746_000259"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1077190.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IW746_000260"
     CDS             complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IW746_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1077191.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IW746_000261"
     CDS             complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IW746_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1077192.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30217..30744)
                     /locus_tag="IW746_000262"
     CDS             complement(30217..30744)
                     /locus_tag="IW746_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1077193.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30924..31709)
                     /locus_tag="IW746_000263"
     CDS             complement(30924..31709)
                     /locus_tag="IW746_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1077194.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31702..32571)
                     /locus_tag="IW746_000264"
     CDS             complement(31702..32571)
                     /locus_tag="IW746_000264"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1077195.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32572..33951)
                     /locus_tag="IW746_000265"
     CDS             complement(32572..33951)
                     /locus_tag="IW746_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1077196.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IW746_000266"
     CDS             complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IW746_000266"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1077197.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IW746_000267"
     CDS             complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IW746_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1077198.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IW746_000268"
     CDS             complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IW746_000268"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1077199.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IW746_000269"
     CDS             complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IW746_000269"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1077200.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IW746_000270"
     CDS             complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IW746_000270"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1077201.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37909..38133)
                     /locus_tag="IW746_000271"
     CDS             complement(37909..38133)
                     /locus_tag="IW746_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1077202.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38186..39148)
                     /locus_tag="IW746_000272"
     CDS             complement(38186..39148)
                     /locus_tag="IW746_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1077203.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39158..39598)
                     /locus_tag="IW746_000273"
     CDS             complement(39158..39598)
                     /locus_tag="IW746_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1077204.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39904..40869
                     /locus_tag="IW746_000274"
     CDS             39904..40869
                     /locus_tag="IW746_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1077205.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40926..41156)
                     /locus_tag="IW746_000275"
     CDS             complement(40926..41156)
                     /locus_tag="IW746_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1077206.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41242..42129)
                     /locus_tag="IW746_000276"
     CDS             complement(41242..42129)
                     /locus_tag="IW746_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1077207.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42218..43441)
                     /locus_tag="IW746_000277"
     CDS             complement(42218..43441)
                     /locus_tag="IW746_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1077208.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43458..44537)
                     /locus_tag="IW746_000278"
     CDS             complement(43458..44537)
                     /locus_tag="IW746_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1077209.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="IW746_000279"
     CDS             complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="IW746_000279"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1077210.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46395..46661)
                     /locus_tag="IW746_000280"
     CDS             complement(46395..46661)
                     /locus_tag="IW746_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1077211.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47140..49374
                     /locus_tag="IW746_000281"
     CDS             47140..49374
                     /locus_tag="IW746_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1077212.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49522..49842
                     /locus_tag="IW746_000282"
     CDS             49522..49842
                     /locus_tag="IW746_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1077213.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14252 a        10711 c         8729 g        16193 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattaaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag
    15181 ttacatatcc aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc
    15241 ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct
    15301 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
    15361 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
    15421 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
    15481 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
    15541 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
    15601 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
    15661 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
    15721 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
    15781 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
    15841 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat
    15901 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
    15961 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
    16021 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
    16081 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
    16141 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
    16201 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
    16261 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
    16321 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
    16381 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
    16441 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
    16501 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
    16561 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
    16621 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
    16681 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
    16741 atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa
    16801 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
    16861 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
    16921 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
    16981 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
    17041 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
    17101 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
    17161 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
    17221 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
    17281 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
    17341 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
    17401 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
    17461 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
    17521 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
    17581 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
    17641 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
    17701 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
    17761 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
    17821 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
    17881 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
    17941 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
    18001 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
    18061 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
    18121 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
    18181 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
    18241 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
    18301 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
    18361 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
    18421 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
    18481 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
    18541 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
    18601 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
    18661 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
    18721 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
    18781 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
    18841 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
    18901 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
    18961 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
    19021 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
    19081 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
    19141 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
    19201 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
    19261 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
    19321 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
    19381 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
    19441 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
    19501 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
    19561 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
    19621 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
    19681 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
    19741 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
    19801 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
    19861 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
    19921 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
    19981 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
    20041 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
    20101 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
    20161 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
    20221 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
    20281 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
    20341 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
    20401 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
    20461 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
    20521 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
    20581 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
    20641 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
    20701 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
    20761 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
    20821 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
    20881 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
    20941 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
    21001 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
    21061 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
    21121 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
    21181 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
    21241 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
    21301 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
    21361 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
    21421 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
    21481 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
    21541 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
    21601 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
    21661 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
    21721 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
    21781 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
    21841 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
    21901 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
    21961 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
    22021 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
    22081 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
    22141 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
    22201 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
    22261 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
    22321 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
    22381 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
    22441 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
    22501 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
    22561 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
    22621 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
    22681 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
    22741 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
    22801 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
    22861 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
    22921 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
    22981 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
    23041 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
    23101 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
    23161 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
    23221 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
    23281 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
    23341 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
    23401 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
    23461 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
    23521 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
    23581 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
    23641 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
    23701 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
    23761 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
    23821 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
    23881 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
    23941 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
    24001 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
    24061 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
    24121 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
    24181 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
    24241 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
    24301 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
    24361 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
    24421 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
    24481 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
    24541 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
    24601 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
    24661 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
    24721 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
    24781 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
    24841 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
    24901 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
    24961 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
    25021 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
    25081 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
    25141 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
    25201 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
    25261 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    25321 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    25381 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    25441 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    25501 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    25561 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    25621 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    25681 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    25741 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    25801 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    25861 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    25921 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    25981 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    26041 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    26101 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    26161 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    26221 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    26281 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    26341 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    26401 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    26461 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    26521 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    26581 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    26641 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    26701 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    26761 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    26821 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    26881 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    26941 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    27001 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    27061 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    27121 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    27181 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    27241 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    27301 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    27361 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    27421 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    27481 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    27541 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    27601 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    27661 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    27721 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    27781 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    27841 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    27901 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    27961 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    28021 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    28081 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    28141 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    28201 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    28261 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    28321 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    28381 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    28441 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    28501 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    28561 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    28621 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    28681 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    28741 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    28801 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    28861 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    28921 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    28981 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    29041 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    29101 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    29161 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    29221 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    29281 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    29341 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    29401 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    29461 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    29521 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    29581 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    29641 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    29701 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    29761 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    29821 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    29881 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    29941 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    30001 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    30061 gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    30121 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    30181 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    30241 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    30301 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    30361 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    30421 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    30481 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    30541 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    30601 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    30661 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    30721 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    30781 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    30841 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    30901 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    30961 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    31021 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    31081 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    31141 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    31201 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    31261 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    31321 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    31381 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    31441 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    31501 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    31561 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    31621 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    31681 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    31741 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    31801 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    31861 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    31921 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    31981 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    32041 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    32101 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    32161 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    32221 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    32281 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    32341 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    32401 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    32461 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    32521 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    32581 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    32641 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    32701 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    32761 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    32821 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    32881 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    32941 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    33001 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    33061 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    33121 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    33181 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    33241 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    33301 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    33361 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    33421 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    33481 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    33541 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    33601 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    33661 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    33721 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    33781 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    33841 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    33901 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    33961 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    34021 ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg tataaggtat
    34081 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    34141 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    34201 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    34261 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    34321 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    34381 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    34441 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    34501 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    34561 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    34621 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    34681 gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc aacgtttcgt
    34741 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    34801 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    34861 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    34921 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    34981 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    35041 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    35101 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    35161 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    35221 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    35281 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    35341 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    35401 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    35461 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    35521 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    35581 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    35641 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    35701 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    35761 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    35821 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    35881 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    35941 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    36001 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    36061 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    36121 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    36181 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    36241 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    36301 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    36361 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    36421 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    36481 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    36541 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    36601 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    36661 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    36721 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    36781 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    36841 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    36901 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    36961 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    37021 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacctg ctgttcaagt
    37081 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    37141 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    37201 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    37261 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    37321 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    37381 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    37441 gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    37501 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    37561 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    37621 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    37681 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    37741 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    37801 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    37861 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    37921 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    37981 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    38041 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    38101 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    38161 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    38221 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    38281 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    38341 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    38401 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    38461 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    38521 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    38581 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    38641 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    38701 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    38761 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    38821 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    38881 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    38941 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    39001 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    39061 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    39121 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    39181 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    39241 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    39301 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    39361 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    39421 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    39481 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    39541 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    39601 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    39661 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    39721 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    39781 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    39841 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    39901 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    39961 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    40021 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    40081 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    40141 ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag taattataat
    40201 tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac
    40261 gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat
    40321 caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta
    40381 caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat
    40441 aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc
    40501 gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc
    40561 caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca
    40621 gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt
    40681 acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg
    40741 aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa
    40801 aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa
    40861 ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct
    40921 aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg
    40981 tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt
    41041 actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct
    41101 gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga
    41161 ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt
    41221 ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca
    41281 aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata
    41341 tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa
    41401 tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt
    41461 taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt
    41521 ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat
    41581 tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc
    41641 agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc
    41701 ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc
    41761 accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat
    41821 tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc
    41881 tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac
    41941 cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc
    42001 tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg
    42061 tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc
    42121 aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc
    42181 tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt
    42241 ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat
    42301 aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat
    42361 cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa
    42421 taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga
    42481 acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt
    42541 ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt
    42601 tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta
    42661 actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa
    42721 ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga
    42781 tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac
    42841 ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt
    42901 caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg
    42961 tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag
    43021 acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt
    43081 cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct
    43141 tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt
    43201 tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa
    43261 taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg
    43321 tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga
    43381 tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca
    43441 tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact
    43501 ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt
    43561 cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc
    43621 tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct
    43681 tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa
    43741 gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca
    43801 cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca
    43861 ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga
    43921 ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt
    43981 cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg
    44041 aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc
    44101 ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa
    44161 taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt
    44221 acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa
    44281 tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat attatgacta
    44341 ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa
    44401 gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat
    44461 tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca
    44521 cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta
    44581 ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac
    44641 aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac
    44701 ttcttccatc gtatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt
    44761 gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc
    44821 atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca
    44881 catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa
    44941 aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc
    45001 cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa
    45061 cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc
    45121 agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc
    45181 tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac
    45241 agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa
    45301 actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata
    45361 tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat
    45421 accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga
    45481 atccatatac aggaattcag tacggtacag accaactgct tctccaccat tattatggac
    45541 accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc
    45601 tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc
    45661 acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc
    45721 gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc
    45781 aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc
    45841 aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt
    45901 caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac
    45961 gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg
    46021 catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt
    46081 tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat
    46141 ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa
    46201 gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc
    46261 atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc
    46321 cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt
    46381 tagcatttca accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc
    46441 attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca
    46501 cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt atactctaag
    46561 tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg
    46621 atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa
    46681 gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat
    46741 gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc
    46801 aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt
    46861 tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat
    46921 tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt
    46981 ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc
    47041 ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat
    47101 atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc
    47161 aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat
    47221 tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg
    47281 gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc
    47341 caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac
    47401 aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga
    47461 atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac
    47521 gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt
    47581 ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat
    47641 ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta
    47701 gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag
    47761 atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat agtcggtgct
    47821 ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga
    47881 ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac
    47941 gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg
    48001 atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact
    48061 gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg
    48121 ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa
    48181 atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta
    48241 gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt
    48301 caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat
    48361 atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa
    48421 cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac
    48481 gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat
    48541 cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg
    48601 aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct
    48661 gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga
    48721 tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg
    48781 ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat
    48841 gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt
    48901 atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc
    48961 gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg
    49021 aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc
    49081 gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa
    49141 gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct
    49201 cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt
    49261 gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt
    49321 acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag
    49381 ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag
    49441 tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa
    49501 tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt
    49561 gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat
    49621 accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa
    49681 cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa
    49741 gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc
    49801 tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat
    49861 aattaaagag cgtgggacaa aagta
//
LOCUS       DACEND010000006        49097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEND010000006 DACEND010000000
VERSION     DACEND010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.006"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW746_000283"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW746_000283"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1077214.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IW746_000284"
     CDS             complement(860..1678)
                     /locus_tag="IW746_000284"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1077215.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW746_000285"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW746_000285"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1077216.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IW746_000286"
     CDS             complement(2967..3923)
                     /locus_tag="IW746_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077217.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IW746_000287"
     CDS             complement(3923..5056)
                     /locus_tag="IW746_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077218.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IW746_000288"
     CDS             complement(5049..6611)
                     /locus_tag="IW746_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077219.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IW746_000289"
     CDS             complement(6823..7905)
                     /locus_tag="IW746_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1077220.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IW746_000290"
     CDS             complement(7971..8369)
                     /locus_tag="IW746_000290"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1077221.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW746_000291"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW746_000291"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1077222.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IW746_000292"
     CDS             complement(9098..10399)
                     /locus_tag="IW746_000292"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1077223.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IW746_000293"
     CDS             complement(10540..11556)
                     /locus_tag="IW746_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1077224.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IW746_000294"
     CDS             complement(11864..12469)
                     /locus_tag="IW746_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1077225.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IW746_000295"
     CDS             12665..13588
                     /locus_tag="IW746_000295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1077226.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IW746_000296"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IW746_000296"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1077227.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15280..16473)
                     /locus_tag="IW746_000297"
     CDS             complement(15280..16473)
                     /locus_tag="IW746_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1077228.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16500..16700)
                     /locus_tag="IW746_000298"
     CDS             complement(16500..16700)
                     /locus_tag="IW746_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1077229.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            complement(17198..17428)
                     /locus_tag="IW746_000299"
     CDS             complement(17198..17428)
                     /locus_tag="IW746_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077230.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(17425..17847)
                     /locus_tag="IW746_000300"
     CDS             complement(17425..17847)
                     /locus_tag="IW746_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1077231.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18378..18731
                     /locus_tag="IW746_000301"
     CDS             18378..18731
                     /locus_tag="IW746_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077232.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(18789..18977)
                     /locus_tag="IW746_000302"
     CDS             complement(18789..18977)
                     /locus_tag="IW746_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077233.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19075..20994)
                     /gene="tet(M)"
                     /locus_tag="IW746_000303"
     CDS             complement(19075..20994)
                     /gene="tet(M)"
                     /locus_tag="IW746_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1077234.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            complement(21010..21126)
                     /locus_tag="IW746_000304"
     CDS             complement(21010..21126)
                     /locus_tag="IW746_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1077235.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(21371..22300)
                     /locus_tag="IW746_000305"
     CDS             complement(21371..22300)
                     /locus_tag="IW746_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077236.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(22317..23339)
                     /locus_tag="IW746_000306"
     CDS             complement(22317..23339)
                     /locus_tag="IW746_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1077237.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(23336..25363)
                     /locus_tag="IW746_000307"
     CDS             complement(23336..25363)
                     /locus_tag="IW746_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077238.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(25360..27813)
                     /locus_tag="IW746_000308"
     CDS             complement(25360..27813)
                     /locus_tag="IW746_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1077239.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(27797..28192)
                     /locus_tag="IW746_000309"
     CDS             complement(27797..28192)
                     /locus_tag="IW746_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077240.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(28264..28902)
                     /locus_tag="IW746_000310"
     CDS             complement(28264..28902)
                     /locus_tag="IW746_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077241.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(28958..29452)
                     /locus_tag="IW746_000311"
     CDS             complement(28958..29452)
                     /locus_tag="IW746_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1077242.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(29519..30298)
                     /locus_tag="IW746_000312"
     CDS             complement(29519..30298)
                     /locus_tag="IW746_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1077243.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(30340..30561)
                     /locus_tag="IW746_000313"
     CDS             complement(30340..30561)
                     /locus_tag="IW746_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077244.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(30558..30848)
                     /locus_tag="IW746_000314"
     CDS             complement(30558..30848)
                     /locus_tag="IW746_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077245.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(30845..32029)
                     /locus_tag="IW746_000315"
     CDS             complement(30845..32029)
                     /locus_tag="IW746_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1077246.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(32211..33614)
                     /locus_tag="IW746_000316"
     CDS             complement(32211..33614)
                     /locus_tag="IW746_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1077247.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(33636..34409)
                     /locus_tag="IW746_000317"
     CDS             complement(33636..34409)
                     /locus_tag="IW746_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077248.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(34419..34796)
                     /locus_tag="IW746_000318"
     CDS             complement(34419..34796)
                     /locus_tag="IW746_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1077249.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(34817..35131)
                     /locus_tag="IW746_000319"
     CDS             complement(34817..35131)
                     /locus_tag="IW746_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1077250.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(35331..37124)
                     /locus_tag="IW746_000320"
     CDS             complement(35331..37124)
                     /locus_tag="IW746_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1077251.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(37121..39310)
                     /locus_tag="IW746_000321"
     CDS             complement(37121..39310)
                     /locus_tag="IW746_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077252.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(39444..40634)
                     /locus_tag="IW746_000322"
     CDS             complement(39444..40634)
                     /locus_tag="IW746_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ1077253.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(41506..43137)
                     /locus_tag="IW746_000323"
     CDS             complement(41506..43137)
                     /locus_tag="IW746_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1077254.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(43151..44824)
                     /locus_tag="IW746_000324"
     CDS             complement(43151..44824)
                     /locus_tag="IW746_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1077255.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(44837..45154)
                     /locus_tag="IW746_000325"
     CDS             complement(44837..45154)
                     /locus_tag="IW746_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1077256.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(45179..46579)
                     /gene="lacG"
                     /locus_tag="IW746_000326"
     CDS             complement(45179..46579)
                     /gene="lacG"
                     /locus_tag="IW746_000326"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1077257.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            46816..47601
                     /locus_tag="IW746_000327"
     CDS             46816..47601
                     /locus_tag="IW746_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1077258.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            47604..48296
                     /locus_tag="IW746_000328"
     CDS             47604..48296
                     /locus_tag="IW746_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1077259.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(48570..48941)
                     /locus_tag="IW746_000329"
     CDS             complement(48570..48941)
                     /locus_tag="IW746_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1077260.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT        14451 a        10150 c         8397 g        16099 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcga tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat agttgtagga gagctagaaa tcctcttata
    15241 ccaagggttt ctggctcttc ttatcttttt actgcacttt tattgcactt ttttcatttc
    15301 tttgtttcgc aagtagtcta cagtaaattg attctttggt gaagtatcat aattttgttt
    15361 tgcttcttta aaggaaatgg ctccatttaa tttgttagct acctcgaaat tactgttagg
    15421 ataaagatgt ccatacgttc ctaaagtcgt ctcaatatct tcgtgtccta gacgatcttt
    15481 aatgataagt ggattttctc ccatactgat taacaaggaa gcatgagaat gccgtaaccc
    15541 atggattctg attcgatgga taccagccag cttagcatag cgttcaatag catatgatag
    15601 tgtatgtttt tgggtaggaa taccgttata gctcattaca aagtctgtct gaattagatt
    15661 ttgttgtact tccttccatt cggataaata agttaatgtg catttatcta atacaatatg
    15721 acgaacgctc gcctttgttt ttggttcaac aaatctataa ttcgattgat tcttgtaata
    15781 gagtgttttg ttgatggtta gtactcctga gtcaaaatca atatcttccc attgaatggc
    15841 agtagcttca ccaattcgca ttccagtcat aaataaaaac cacagagaga taaataaaaa
    15901 gtgttgatag taatcttctt tgtaaattag agagattact ttttcaaatt cttcttttgt
    15961 ccaaaattca attttagact tttgcttttt cacattgcca ataatcttag atggatttgt
    16021 ggtggttatc ccaaggacaa ttgctcgatc catagcaact gaaaatagtc cttgaacagc
    16081 tcttacataa gaagacttac acttctttga taattttagt tgccaatttt gcacatcaat
    16141 aggctcaata tcggaaacag ccattttatc aaaataagag aaatgcttct taatagttgg
    16201 taagcgattt tcatatgttc tgagctttac ctgtgtctta taccatggaa ggaaaatttc
    16261 aaagatataa tgtttaaacg tcattttatc cttattctca cttaattctt ctggtttcat
    16321 caatagcagt ttcgaatatt cttctcttgc ttctttttta gtagtgaatc cactacgata
    16381 tttttgaatt tttttccctt tagaatcata acctagattt gcacgaaaat aataggttcc
    16441 attctttgct ttttttattg ggtctttaac catagttttt actccttact gtgcaaaaac
    16501 tactcagacc tgttttcatt tgaaaaccga attcctagaa tttcttcaac agcttcacga
    16561 gggactctat ctagctttcg agattgatag tagatatgcc cttttgtaat catcaattct
    16621 tttgcttttt ttatgatgtc tgctgaaaaa gaagttccat atcctagctc aactaaatcc
    16681 ttttttgtga ccgttatcat tattactcct ttcccaatta taggctagga aaactccaat
    16741 attcacctat aattatatca aatagtttta taataataca ctgacaagag aagaatatct
    16801 tagcaatcga acagctctag caaagctcac aggaatttca cttctgcatg gttctcatgt
    16861 agccgtaccc attgcctgtg acgcttctaa cgctcggact atggctatac gggagtatca
    16921 ttatcacgat aagaaagtca tggcagcaat cctgctaaag attactttcg aattactaac
    16981 atgaatcgct cactaatttt gtaggtcatg gcgtgttagt tcatcagctc ttttcttacc
    17041 gaaacgtatt cgattgcctt tatttggttg tcaaagaacg tttggcttgt tagcctatca
    17101 aaacctactg aacacacaat atgctttgat ggaataacaa acctcccaaa tcgggaagcg
    17161 tggatacgat tcgacacgct tccacgaaaa aatgggacta tttaatttcg aacgataaaa
    17221 tcttagagat gagtttcgtt tccattctgc cacgtagggt ttcatcaata accatatgag
    17281 aatttccata ttcgtctgtc atgcgacgta atgaacgttt tgaggtatac cctcgataat
    17341 gtttaacaat ctgattaatt gcttccacgt cgccatctgt cgcctttaca atgaggggga
    17401 agggaaccct tggataagct gttttcattc tttacactcc ttcatgaatt ctttaatcat
    17461 ggctagtcca cttattcgat gacgataaac agtcgaacga ttcaaattta gtaactccgc
    17521 aatctccaca tcactcatat ccataaagta atgaagtaaa ataatgttcc gttttttctc
    17581 tgaaagatga cgcagggctt cacttaataa atcactttca acattgattc ttaatccaaa
    17641 taatttaaat agttggaaat cagtgacgta cgtatcaaca gtagaaaaag agttgacaag
    17701 gtagttatct atatcagaaa acgagacttc ttttgctgat agtctgtcta gatgtttgaa
    17761 gtaatctttt cgttcatctt caataacttg tttgcagata tagtcaaact gattttctat
    17821 ggtttcttga aaagaagatg gtttcatgct tttcaccccc tttctgtcgt gaaagggagt
    17881 ggcatgtgct tctttatctc ccttcatact aaaccccgac acgaaagggg gatttgttgc
    17941 ataaatgaag agaaatccta aaagatattt attaagtgac caaaaaagca cataaacaga
    18001 ttgttttctc tgttcatatg ctttgatttt tctatctatg taatctgcaa aaccacattg
    18061 atggtcatat ctatccagta caggtggata agtttttttg ataagaactt aattaagaga
    18121 actagatgac agattatctt tctcatggcg acttttccct ccattaagtc gccaatttta
    18181 tctactttct aggagtatga ctaaatttta taaagttgta taggtaaata aaatcagcga
    18241 cctttctatt tgaaattgga ttcatcgcat taataaattc cacagtgttc tataggaact
    18301 cataaactat ataacatgtt tttctatacc tgtttatggt tatataggaa cagtaaaatg
    18361 tatagaggtg atttagtatg cgtaaaaaag aagataaata cgattttaga gccgttggtt
    18421 tagcgattaa agaggctcga atgaaacggg gtctcactcg tgaacaagtg ggaacaatga
    18481 ttgaaattga cccacgttat ttaacaaata tagaaaacaa aggacaacac ccaagtacac
    18541 aagtgctgta tgatcttgta tcattactcc atgtatctat tgatgaattt ttcttaccta
    18601 cagataattt gattaaaagc actcgaaggt tacaggtaga aaaatacatg gatagcttta
    18661 cagacaaaga actatcctta atggaagcac tagcaaaagg tatcaatgaa gcaagaaata
    18721 ttgaaggcta gtcagtaaaa ttcagataaa caaaaagagc cgataagatg agagtttcat
    18781 ttctcaaatt atcggctctg cgtcttggcg tctggctctt tgattgttat tatattcatt
    18841 ttttgaacat taattaaagt ttcgttctta catttggggc agtataaagg gaaattttta
    18901 agtatagtat ccactcgtat tcgtagtctt gttttatttc cacaaatagg acacaatatc
    18961 cacttgtagt ttataataac aatctcctcc tttccacttt aattcaaatc tatattaaag
    19021 aatatttcat cttatttaat aagaaaccat atttatataa caacataaaa cgcactaagt
    19081 tattttattg aacatatatc ttactttatc tatccgacta tttagacgac gggtctggca
    19141 aacaggttcg ccagtggtaa cctgatatcc ttttagctct gctaaacaaa cactaagccc
    19201 atttgtaaaa aaagttaaat cattgcgata atcttgaata catcgagcag gaatttctcc
    19261 aataataatg acctcattat ttttcagttg agtatttacg atatttgcac aatatttggg
    19321 agcatcgtta tatgcccgtg aaagatattc ctgtggtgca taaactttaa aactaagata
    19381 tggctctaac aattctgttc cagcttttct aaaggcttgc tccagtacaa taggagtaag
    19441 catccgaaaa tctgctggag tactaacagg gctatagtat aaaccgtact taaaacagat
    19501 tttacaatcc gtcacattcc aaccatataa tccttgttcg caaccatagc gtatcccttc
    19561 cataactgca ttttgaaatg attgatttaa gtatccaaga gaaaccgagc tctcatactg
    19621 cattccactt cccaacggaa gcggtgatac agataaacca atggaagccc agaaaggatt
    19681 tggcggcact tcgatgtgaa tggtatattc tgcatttttt aacggtctct ccatataaat
    19741 gactgtaggc tcttttagtt ctatctccac atgatacttt tcttgcaaca gtgcactaat
    19801 cacttccatt tgtactttcc ctaagaaaga aagtataatt tcatgtgtcg tagaatccac
    19861 gtaatatcgt agaagcggat cactatctga gatttccaaa agggcatcaa gcaacatttc
    19921 tctctgttca ggtttactcg gttcaacagt tgtttgtagt agagggtgcg gattttcaat
    19981 cttttttctc tgtggcaata gttttgtatc tccaagaaca ctatttaact tcaaaaactc
    20041 attttgcaaa ataacaattt ctccagaata agctctatca atcttacata attcaccatt
    20101 tattgaagta tacatttctg taacttttat tttttctttt tctgatactc taaccgaatc
    20161 tcgtaaatgt agtactccac tataaaggcg tatatatgca agacgttgtc ttttttttgt
    20221 atattcaatt ttgaaaacat ttccgcaaag ttcagacgga cctcgatgtg ttgatgaata
    20281 aaatttatta gtaataactt ctataaggtt atcaatccct atattacttt ttgcacttcc
    20341 atgataaaga gggaacagag aacaattctg aaatcttatg ctttcctctt gttcgagttc
    20401 caatgcttct aatgatttac cggacatata tttctctaaa aggtcatcgt ttccctctat
    20461 taccgtatcc cattgttcag attcggtaaa gttcgtcaca cacatattag gatacagttc
    20521 taccttctgt ttgattacaa tttcggcaga aagtttctct ttaatatcct gataaaccgt
    20581 tgataaatca attccatttt ggtcaatctt attgataaaa aagattgtgg gaatccccat
    20641 tttcctaagt gcatgaaata atatacgagt ttgtgcttgt acgccatctt ttgcagaaat
    20701 cagtagaatt gccccatcta aaactgataa tgaacgatat acttctgcta agaaatccat
    20761 atgtcctggc gtgtctatga tgttcacctt cgtattttcc cactgaaaag aggttattcc
    20821 tgtctgaatt gtaattcctc tctgacgttc taaaagcgta ttatccgtcc tcgttgtacc
    20881 tttgtccacg cttcctaatt ctgtaatcgc tccactgtta tataataagc tttctgttaa
    20941 ggtagttttt cctgcatcaa catgagctaa aactccaata ttaataattt tcatgtgatt
    21001 ttcctccatt caaaaaccca aaagggcata aaaatcccag tgataaatac ttttatcact
    21061 gggattttta tgcataacca taggtataca aagcatacag atattctctg gatactttag
    21121 aatcacatga taaaggtatt cttaaactgg gtacaaaaaa ctaagccctc ctaaaaaagg
    21181 acatctaatt atttgttccc gctatcaaat tgacagttta tttaagaata ccttgccgca
    21241 tatttattaa ctccttttaa atagtcactt aaataatagc acgtaagagc atatttgtaa
    21301 aggaatctcc aattttttat caaaaagagt acatgattac aaagtatctg taatcatgta
    21361 ccaatatttg ttattttaca atcttccaat tatcttgttt ttctaatatc aactcaaatt
    21421 gcgagatttg ggttgccttt gtttcttgat ctaaatattt cacagcgaca ttcacgatta
    21481 cttggttatc ttttctggta aagataggat tcaccaattc ctcgaaaaca tattccttgt
    21541 taatcatagg taacacatga ttacttacat agtaagtcaa ttctttttct gtggcggtag
    21601 gatagagctg gaagaacgtt tctaaaaagc tagtgatttc gtccatcgtt tctgtatcta
    21661 ctgtatggtc actttctagc tgtttgggtt ggtagtccga tttttgaggt ttcttactca
    21721 ttgtcggatt tttgataata accatattat ctgattcatc tatatggaca accacatgaa
    21781 agctagacga tatagtttcc ttgtctttgt cttcggtaat aacttgttca acagaaaaca
    21841 gtacctcaaa ggtgttctca ttcacttgag aaacctgcca cacctgtata tcgttcacac
    21901 tagaactagt aggaatatcc ttacgaatca tttcttcatt taatacctgc aattcttccg
    21961 tcaaataatg ggtcaatttt tcatttcgtt tatcaatggc ttcttgggat tgttgccacg
    22021 aaaagtagtc tttcgcaaag gatttcacaa aactttctac ttggttggta tcgacaatct
    22081 gctgtttaat gatttctgtt tcacggacag tatgagtgtc aatggcagta aaatttttat
    22141 agattccaaa tccgacacta ccaatcaata aaacccataa aacaaaggtt aatttcttat
    22201 gcgtgccaac tttaatcatc ggaacttttc tttgcttgct cggtttttta gatttctttg
    22261 gtttctcctt cttttctttt cgctcgattt ttataatcat ttgattcatt cctttcttag
    22321 ttatgaataa cacgtctagc cccaatcaga tggtcttgcc aataacgact gcttaagtcg
    22381 ccataaccga ttgggtcgcc tgcatgataa aagcgatttc cctctaaata tatcgccaca
    22441 tgagtcacat atgtgcctgc attataggtt gaatgaaaga agataaggtc gcctgcttgg
    22501 gcttcttcca tagagatttc ttgcgtagcg tcatactgat cttgggcgac acgagggaga
    22561 gaaatgccag ctttgtcata cacccattgt attaagccac tacaatcaaa agaggtggta
    22621 ggcgacgccc ctccaaagac atagggaaac ccttcgtatt tcaaggcttc gtccataatg
    22681 acttgcacgg tttcaccatc aaattctgtt ggggaagtgt cggttaggta ttgtgaaacc
    22741 aactgaacat aaaactgatt tccatagtta tatcgccaac caccattcac aggtatggct
    22801 ataggattgg gatagtctgc tttttgacca cccgacttct ctttagaaaa ctgttcggct
    22861 aattcatagg tatatttttt cccatgactc ctcacataat ttaaaaagcc accgccataa
    22921 ttataagatt ggataacaga atctatatcc actccttgtt gttctgcact ggttaataat
    22981 tcactgaaat atttaacccc ttgtttgatg gactcttcgg tactcaaact gttgggcggt
    23041 aaacccaaag attcggaact ttgcataaca tcttctgctg taccgcccga ttccacttgc
    23101 ataatggcta agatataaga aacatagtct tcaatcccat attccttacc atatttttca
    23161 atcaacggac ggtgtgccaa cacttctggt gaaacggtca caccgccttg tggaatagaa
    23221 gaatccttgt tgttttcgcc actgtccgta tcatctgcaa agaaaatgac taggaataat
    23281 aagagaaaaa agaagagaga gaagaccaca ctacaaatca ctgttatttt tagcttcatg
    23341 tttgttgtcc tcgcttatcc gttcttttat ttagcttcct ctgattgaat tgcttcggtg
    23401 gtgtcttttg tttttgctct tttttcaatg gtcgctgatg attttcacgt ttagtgtttt
    23461 gtgacaaaac aggcgtgacg gtttgctgac gcttgcgaac atgctgctgg ttattctgaa
    23521 caggagatga tgggttttct tttcgtcttt tttcttttgc tttatccagc tccaaacgct
    23581 tttgagcaat gttctttcgt ttttgctcct gctggttcat acgctgttgt ttgcgttgag
    23641 ctttggtatc tgccatagat tgtttaaagt ctgttacatt atgagtgaca ttctcttttc
    23701 cttgatgaag ggcatatttg acatgggttg gtgtcgcctg tatctgttgt tttgcctgct
    23761 ttcttttatt gactagacga tttttcgtgt ccaatacatg ggctgtcttt tcacctaatc
    23821 gttgcccgat accgccttgt tgctttttca cattggaaag tgctgtttct ttctttggtg
    23881 tagtccgttc attttgacga gtatggttag ctttattggt ttgattctgc tttgttgtca
    23941 ttgtcttttg cttcgttgac cctgcttttc cacgaaaaat cttactcata ctgcgttgaa
    24001 atcttctggt ttgtcgattc atcatttgac gaggtctgcg aaacacttgt ctgcccatgt
    24061 tttgagagtc gttgctttgt aaagtgaata ggctcattaa atcgcccagt ttaaaataaa
    24121 tccctgcaaa ggtcacaatt tgtaaaaaca taatcatgaa aaagggagaa cttgctgaca
    24181 gcgaataaag catagtggaa atactaaagg caacggtaat aatcaaggta ataccagccc
    24241 ttgtcataat cacattaaat aacttggtaa tggcatgcct gcccatgcca tcaaaactcg
    24301 gcaacatact taataggaaa ctgattggca aaaacatagc gaaaataata aatagcactt
    24361 gcgaaaaaat catgattcct gttagaagga aaacaaaaat cgagatacca atattgaata
    24421 caaacaaaaa gaagactgtt cctaatctgg aaatcgtctt cggtagggtt aaataggtat
    24481 tgtcatgatc ttcaatttct tcaatcacta cattttctct atcttcacca ttgtttgtat
    24541 ctggactggt agacaataaa ctttccacac gttcttcccc taacgcttct acatcagtgg
    24601 agccatattg aagcaatagc catggttgct ttacttgaac agagaataag ctgtcacgta
    24661 tcatgtccac actgtctttc ccttgactat ccgtgttagg aagaacaatt tttgtcccaa
    24721 tattcagact ggcggtactg acatctttgg aaaaatcatt gattttcgca atataatcgg
    24781 gtgcatacgc aataaaggaa gccgatagaa taaacaccac gacaaagttt aaaacagctc
    24841 gtatcacttt cgtcgtttct cgcttaacca atcctgtata ggcaacatag attccaacaa
    24901 tgagaataaa aagaaggagg aaaccgacat aaaaccctga actggaaaag ccattcggtg
    24961 taatgcctgc aatcgtctgc atgttttcgc caattgcttg ggatgtttga ctaatgaaat
    25021 ctaaggcata ggcttctttc accaaataac ctgtggcgtt cgatagataa agagaaattg
    25081 tccaaataaa attagtaatg gcatacaatc catacatcac cgattgtcct atgccatcac
    25141 tccaattcca tggaagccag ccccagctat tgtccacata ataatcaagc tgatattgat
    25201 tcaaaccata cttggagtat tcattgcttg tatcaatggt ttcatcaact aagcctgtcg
    25261 cttgtactgc attgcccagc gtgcctaata aaagtaaaat aacgcccaca caaaggagcg
    25321 ttatccaact atagtgaaga aatttctttt gtttttgtgt cattcttcca cctcatttcg
    25381 cacaggcggt ctggtatcaa aggcatgaag caactcttca aaaatcgggt gaaactgcat
    25441 aacccctaca cgaccataga tgtctgaaac taaacattgt ccattttcta attcacgtaa
    25501 gcgtttctga ttgttttcat cttccttgtc cacaccaaaa aattctaagg tctttttgat
    25561 ttccactagg tcagtggaac gaaaggcaaa tttcaagcca atattattct tcatcttttc
    25621 atctagtaaa tcgtccgcgt tttgagtaac gaaatagacc cctgcattca tggcacgtcc
    25681 tgctcgaacc aatcgcatag atagagcttt tccttgtgcc acttgtaaaa aactccaagc
    25741 ctcatcaagg tcaacaattt taaaaatgga acggtcagag tggataaagt ctaaagcaaa
    25801 ggtactaatg acaatcagca ttgccacact taataattcc atggtagtgt attcttcaaa
    25861 agagctattg gaatcgggta agaccaaatc tgccacttga ataatattga gttgtttttc
    25921 caagctaatg gattgggtga cactgccatc actgaataac agatgggcaa aatcataatc
    25981 gacaaagctt tcgatatggt cagcaatact attgctgatg ggcgtatctt cttttcgcaa
    26041 ttcatcaatc accaataaca accctcgttg tttgctttga gttacactcc gaatagcttt
    26101 acgcaggaca ggaaacttct cgctatctcg gctggaaata cctgttaaga acgtgagaat
    26161 atcaatggca aggctttctg aatctttcgg gagttttaaa atcacataag ggtcaagtaa
    26221 ccctttattg tcgttctcac tcgtgagatt aacaatattg atttcatggg caatatcagg
    26281 taaggtttct ttccattcac cacgttcagc ttttgggtca acaatggttg cctgtccgcc
    26341 aaagagtact gaataataga ccagcatatt gttagagaat gattttccgc cacctaatga
    26401 accaataaaa gcggacgcta atgcatttgt tacagagcct ttaattcctt gacttgcaag
    26461 gctaggttgg agataaatat ttctgcctgt atcgagattg taaccgacgt aaataccaga
    26521 attttcacca atcatttgtg tagcaccgaa accaagacca gccaaaaaat ccgacgtgac
    26581 atattgaata tagtcattga tatatcgctt gcttgaggga ataaattcat tatgcaagcc
    26641 tagcatatcg ccaaaaggtc gtactaattt gatagacaga tcatcataaa aatcttttac
    26701 ttcatcacaa cgttgtttca gctcgtccaa attctttgct gatacccgta ccacatagct
    26761 tagtttgtac atagattctt tggtttggtc taaattactt tctaattcat cgacagattc
    26821 taaagcgtcc accacattag tactggtttc gttgtcgctt tcccatgcgt ggctatctaa
    26881 atctttcagt tctttctttt tattgcgaac ggtagataag gcttttttat tggcgacaat
    26941 ttcaacattc atactggtat caacggggaa agtaaactgc tgttgctggt aataaaaaat
    27001 ttcactatta gggaaatcca attcaccaac aatactatta attgtaaaat acgccacata
    27061 agtagtggta tcttcctgtt caatccgtaa atagcgttga ttttcttcga ttaaacagcg
    27121 agtgggtttg agaatatcgt atttctttac caacactttt ttatcttcgt atcgtttcgg
    27181 taaatggtag tcgtagtcat caaaagggac acctgtttgc ccgtaaagat gttcaataat
    27241 ataaccaaaa tcatctttct ccaatttacg aatctgaaag cgtctggata atttactttc
    27301 taacaactgt tccatcttag aaaagcgttc gatttccgcc ataggcatag aaacaaaatc
    27361 gcccattaat ttgtgattca caccatgaaa gaattccgca aatgcttttt gaatttcttt
    27421 cccaacattt tttacggata cttcctgttc attgaggtag agtttaaaac caataaaaaa
    27481 gcgatagtct acttgattgt caccaatcat agaaattaac gcttcggtct gttcatctat
    27541 tttttcgtaa gctatctctt ttaatcgtcc agtgatttct tttttcgatt gttcctgtgt
    27601 ggctcgaata cttgattcgg ttgctagttg cagagcatga atcttgccat cacgattttg
    27661 ggcaatcaat tgacagaaat tatcatgaac ttgaattttt tcatcggaac ttaaaaaact
    27721 gtaattatag ggaattaatt cataataagc gaatacttcg ccatcatgat taaatacgag
    27781 attatcttcc atatatttaa tcggatatgc catctagttg ttcactcctt acaatcgtaa
    27841 tcgattcatt cattgtttct ttttttaatc gtatttgttt gccagcgtag gttactttag
    27901 ggcgtagagc ataggtaatg atagacttta gaaatccata gggtttcttt ccgtcaaagg
    27961 tcttttggca catgaaccca gtgagaacaa tgggaatccc taagtatttg agtagtgctc
    28021 cgtcaatcag agaaagtggt ggtaaactgc caaagagtat gacgacaaac aaagaaacga
    28081 taaaccatgt catttgcgta aaggtaattg ggaaagggag ttgtaaatca ttaatcgcat
    28141 aaatcacttt ttctactgac caaatactgg tgtaacttct tatttttttc atgtcttcac
    28201 gtcctttccg taaaaataga aaaagaacaa tcaaaatagg tgattgctct ttcaatgctt
    28261 taactattca attttatttt ttgtccattt tgcaataata ccttcatcag tttcttcatc
    28321 atctggattc attgaatatc tgaaacttaa attggtatct gaccctaatc gttcatataa
    28381 gctgggtcta tacaatctcg ttttattgtc attaaaagga cttcgttttc ccagctcacc
    28441 atttcccttt aaaggatttc ttctaactga aaaacaatat ttttttctgg gatcttctga
    28501 atcactttga cttaatgaga aacacatggc ttctttttgg tcttcatttt tcttctcact
    28561 aacttctgtc ggaataaatc gagcaagagt ttggctaaat tgaaataaaa catctcccaa
    28621 gttactactg tattcgatac caaaatattc tcttattgtt ttcgtgtctg tatataattt
    28681 taccgaatta gcatagacag ctagcatatc tgaaaaatcg ttttccttta aaaattctag
    28741 ttttaacaga gcatattctg gaactttttc ctctttttca aatagcttaa caagtacaat
    28801 ataattttgc tgtttgtaac gaaagtaaaa ggaatctatc acccagcctt tatctttcat
    28861 gtctagtttt aatagtttaa gattttcaaa tacatttacc ataatttata cctctccgtg
    28921 ctatttatta atttaattat aacacggatt gttaaactta ctgacaatat tcaaataccc
    28981 catgagaggt aactaggtaa ttaccctcta tttccatatc acgtcccaaa gattggtagt
    29041 caatataatt ttgtaaattg gaagggactt caccaagctg tcctgtttct tccacgtaat
    29101 aacgggcgac atcttccatc gaatcacaat cactataaca atgaatatcc tctttgtttt
    29161 ccagtaattc ttccaagcta ctaaaccaca tgccttgaat ttctttgagt tcattataaa
    29221 taggtgtacc ttcgatttct tgaatggctt cacatagacg gttgatttca ctaatcggcg
    29281 tgtattcgtc cacatcaaaa ggaagctcaa aatcatgtat tgcatattct tcgtagtcct
    29341 catttaaacc gattctttct gccatttctt cctcatcaat tggtggagta aaccatgctc
    29401 cgactaattc accttcatta taccgtccta aatttgccac atagactttc atttccaatt
    29461 cttatcacat cctttttata aaaatagtca taaaaaaagt agcaatcagt taatgacgct
    29521 acttatcaag gtatccgcta atataatctg taactgtact tgaaatacct attattagtt
    29581 ttgcttctct ctttccaata atatttgtat tagggtgtgc aagtgaatgc ctatttcttg
    29641 cttcattaag agagctaatc attccatttg atgaccttat cgttgtcttc actatatcag
    29701 caagttcagt aggttgaatt tctttttcaa ttaattgttg tatcctacta tataatttag
    29761 atagattttc gtctctagga actgtaattt catatttttt tagtatttca attaaatatc
    29821 catgaaaagc agtgtgaact ctgtcaaagg cacgttgata ttcaccatta ctcataaaag
    29881 actcagcgtc ttcaatagct tgtgaaacca catcactaat tttcaactga ctaaagttaa
    29941 ttacttcgta attctctgga agtggtacta aaatagtatc cactaataca ttatcacctt
    30001 gtttatcata tatggagctt gctgtctggg taatcaaatc tttatgactt tcaaaaaagt
    30061 ctaaatcatc aaaaggaact cttattttaa gatatgtgca aagcgtattc cagccctttt
    30121 tatatccttt ataataagta aaatcgtcag tctcatctaa agttacctct gatattttca
    30181 ttttagaaac aatctcgtta cctccttcat aataatttga agagctcttt ctttctaatg
    30241 ttttaattaa aagagattta aattgatcat ttgtaaacat attcataaat cctttcataa
    30301 caaaatatat acttcctatt ttatcatgaa aaatatctgt taagcaccaa tgattcggtt
    30361 aaataagttt aataacacat ctttcacgcc agctgcattg aacacaagtc cgacggcgat
    30421 tagagcaata atcaagaagc caatgagttt tgaaaattct cgtttaaaac ctaaaaacag
    30481 tccaatgaca acaatcgcta acaataccaa tgactgtgca ttatccaaga accagttgta
    30541 caaattctgc ccaaaattca tgtgattgtc ctcctttcct taagatattt ttctcgataa
    30601 tagatatagc tttttacacg ccttaataat tgaaaagggg ttaaatgctc tccatagacg
    30661 gttactcgat tggtataatt attataaaat ctcaccttgt aaaaatcata ggtatagatg
    30721 gtatcaccag cttgccgttt tccctcaata atgagagacg tatatttccc tgtattttgt
    30781 atatacgctt gaaactcaat gccacactga aaccctaact tctttaattc ttttgttaaa
    30841 aggctcatgt aatcaaatcc tccatggcta gatgttgttg ttcaattaat ttctcatgtt
    30901 tttctgtcag tttggcttcc tgaatcatgg tggagataat ggttgtaccg ttgagaacat
    30961 ctaaaatgct tgcgattttt aatgtagggg cgacttgatg atgtaaccag ttcaatgttc
    31021 gttcaaaaga ataaggctct ggctgtgtgg ttaggcgtaa tgctccacga tttttaccga
    31081 taaaccattc ccaacgttca ttagttttcc aatcactccg acgtttagtg tcgtctttat
    31141 ctgcaaagcg gatatagcga ttgataattt caaaggctgt tttttccgcc tgttgatgtg
    31201 ccaataaatc tttcatggct tgggtggctc ggtcattttt taaccgaatt tcaaaccgat
    31261 tctttacttc tgcgtcttct aagggaacat catttttcac gtattgctca taatctttct
    31321 catatataca gaaatagact tcacttttta atgagccaat ataaagtgtg tttcccatgt
    31381 atggtttttc atcacggtga accaattcac cactgcgata atttttaaag cttcgaaaga
    31441 cggaaatgca ttcttcctgt ttacatttct ttgccaaatc tggaatattc aaaatccctg
    31501 ttttatcgtt gatagctaaa tccaagcgtt tgaaaatacc tttatgttcc atgcaatctt
    31561 gaaagaaatc ataccagctc cgatgttgag ccaatagaaa gttttcaaat tgccgacagc
    31621 cttttccttt cagttctaat aaaacacctt tacttacatc atgagagact aggacaacaa
    31681 tatcaccgaa acgatagtgt tcgggataag aataatagcc aaagtcttcg tgtagcatat
    31741 aatccatatt caaacgtaaa acctcaccga taatttgagc cacatcattg gtagggaatc
    31801 gaatccgtac atagtcgaat aacatctcca atgggttatc gggattgaag cgttccaaag
    31861 cgttcagtaa atcttctttc acctgttgag tagggactgc cttgcctgtt tcaatatccg
    31921 ataggtaagg tctggtaata tgagaagcaa ccgccagttt attttgagaa acgccataag
    31981 ccagtcgctt ttctttcaat tccttaatcc aaattttttc gttttccatt cgcaaacctc
    32041 caatctaaaa aagatgtaaa ctatgaccct tgagtttaca cctttcatat tttccgcaat
    32101 cctttgtacc atcaacattg gttgatgaga attgcctgtt ttcagttgta tttgtgcccc
    32161 cctgttagat agagggggct aaaggattgg cgtggctaac gccacaccaa tcaaagcaag
    32221 tctgaacctg tacctttcac ttcgtgcggt acaggctcag attgcttttt ttgaatgact
    32281 tttcctattt ctcctaagaa atcgtacctt tttggtacaa ggggtgtata aaactcacta
    32341 atcacacttt ttcctgtatc cacgtaacct cttcccttga tgggttttaa gaaaaattgt
    32401 ttttgtgtat cgcttccgaa catcatacca aagcccattt cagacatacg acccaaagcc
    32461 acacggaagt taaagttgtc ccgaatccca tcactaaaat attttgcgtc tggacgttga
    32521 caggcaagaa ttaaaaagaa gcctgcttgt cgtcctaaca ttacaatttg tttgagttta
    32581 tgaataacgg tggtactttc acgacctagc atatccatca ttgccacata ttcatcaaaa
    32641 ataagaaaat tggcagacaa accaagataa gcataatttt ctcctgtttt atagttaggg
    32701 tgttgtttca tggtttcact gcgatcaatc atatcttcgt aaaattgatt gatacatgcc
    32761 aacatatctt ctttacggta atagacatta ttcatgaccg cccctaagtc tgccaaatca
    32821 gcatttttag ggtcaagaat ataaagcttt gcatttgtat gcaggagaga ttcaatcaat
    32881 gtgagaataa aataggattt tccactacca gtgcccccag caattaacat gtgagggaga
    32941 gaatcgaact cccaatagac cgattccatc aatttgacac gtccatcttg agctaccacg
    33001 tcttcaatgc caattcgatt gccaatagta tcataaagta ggacatactc tacataagaa
    33061 tctttcaacg ttttttccac taattcacaa tacaatcctg tttctagttt gttttctagg
    33121 tgcaaaagct gatcttgata tttccctaag gtgatttcta catgaatatg gattagtcca
    33181 tctttcattc gataatatat tttaggaaaa tggcggattc gttgtttggt tttcttgctg
    33241 ggtaaatctt taaagaatcc ctctgattga gtttcttcgg cttcatacca atgattttct
    33301 aaaatcatac gagctaactt ttgacggtgc tctaactgtt ttatagaatc tatacgatag
    33361 tgataataaa gccagctgat aagtccgcat acaaaaagag cagtgagaat actgctccaa
    33421 aagtatggcg tatgaagttg atagaaaaat gtatctaact gaactgcttg ccagttcacc
    33481 gattgtaacg ctctccaatg aaaagggagt aggacgacta gaaaaactac cagcaatgaa
    33541 attagtacaa aatgatagac caagtgctta tcgttggctt tgattcgctc tcctctgtca
    33601 tgaagcaatg gcataaaaat cctcctttcc acctatcaaa ataattgtgt attgtagata
    33661 cgagcctgtt tatcaaaagt tggttgagaa atggcttcat atttctgata agcgattcgc
    33721 ttttcaaagc gtaaattttt ttctttcgga taataatgac gtcgatagag agtgtgtcct
    33781 aacagttttt tcgcttcttt tgtcaacgga tagagatagc gaaacatgcg tccattgatt
    33841 ttctcaatcc ctttgtattc gcaaaatgcc tgggaaagcc aatgcttttt ctctacctgt
    33901 tcaaagcgag cattttcctc taataaaagg cgagcacttc gagggtgtat cttttcccat
    33961 gactgcttat cacgatagac acttgtcttg aaatacccgc agtaaaagaa gttggacgct
    34021 tgatagacat agccacattt tccctcaatt ccatctgcca gtgtatataa aaaatgacag
    34081 tccgtatgtt ctttcaacca cttaattaga gctgaaagag cttgactgcc aaaataattg
    34141 gtttgattca tttctggaag aaaacacatc tttccaattt ctaaataatc tgtggtttga
    34201 agggaactgt cgggaaagag tttgcgtatg gtttgtaggg gttgcgtacc ccagcctaat
    34261 tccacaacgc ctaataattt ttcttctgaa aaaatcccta gaaagtattt acacaatcta
    34321 gggagtatct ttgaataatg gtattgccga ataaaagaaa tggcttcatc cttcggaatt
    34381 tctttcaaaa tcaatggtgt gtttgtcata agcaacacct atttttcact ttcggacgct
    34441 tcttttttat atttctgatg aggttttgtc tgtcctttta atacaagatc gtctgctttc
    34501 acgtaccaat caacgtctgc ccctcgaaaa gtagccgtcg ctacggtatc tgccacagga
    34561 ttgattaatt ccacttctgc gttgtagtca aattctttca aaggaacaga agcggggata
    34621 ctgacttgta tcatgcgtcc ttgtcctttt gattttaagt cataggtacg ttctttaatt
    34681 tcttcggaaa ccgtaccatc ttcattctgg atacgaacct cacgacgcaa ggcggaaaat
    34741 ttcaatgtgc caaatgtctg gtctttttca atcacaatac cttctgctaa tctcatattt
    34801 tttcaccttt cctttcttat gctttcacca tatcgtctgc gtgcataatg taattggtaa
    34861 atcctcgatt gccaatttta taaccttctg cggtaatatg tgggttgatg agtttgactt
    34921 tttcgtcgga ttcaaaatgt ttctcgccag cttcggaggg aagaatgaca acaatatcat
    34981 ctgctcgttg aatcgttgag tataaattgt aactacgaga gagaatggtt ggtctgccat
    35041 ttattcgacg ttgttcaatg ttcccttcac cagcatattc taattgtcca aatgtttttt
    35101 ccatattggg aatgacatat ttaagttcca tatttttacc tatcctttct gtttttatgt
    35161 ttgaatgaat cttatttgtg tgatggtttt aaagggggga gagcgacctt ttgattcttg
    35221 attttttatt ttcataagtt cacttccttt caaaattggg taaaaaaata gacgctccgt
    35281 actgagcgtc cgattcctac tatatttaaa acaacgacta cctaggtatt ttaaatatta
    35341 taatcaaatt ctattttatc taataattga gtgggataaa gcaagtctcc tttactcgta
    35401 aaataaccca cctctgagaa aagaaataaa ttattttttg ctcttgaaca aattacgtat
    35461 agtagttttc tcgctgaact aattccttta tcagaaacat taaaaatttc attccaatga
    35521 ggaattttcc cgtttagaag attaatagcg ataacagtat catactcatt tcctttaact
    35581 ttatgtgctg tagtaagtgt gataccatct cgagatttaa acatcttctt aaaactagat
    35641 acactactag ataatgaatt attttttatc cgatcttcta cttcgttaat aaaaaataga
    35701 tagtgcttaa aaatagacgg gtaatttttc tcgtcaatat taagattaac aaatatatgt
    35761 ttggtaacaa atttgtataa atcaacaccg ttgtcgtctc cactatacat tcttttcaca
    35821 ctgtttatta gctgtaataa atctaagtca ataaaataat ctttaacttc tattccaaat
    35881 tcatttttta atataattaa aatatcgttt gcaattagct ttcttcttcg aaccttttca
    35941 cctgattcag taaagtacaa aattgctaac ttgaaaaata aactatgatt atttacctta
    36001 attggtgata tatttatagc atcaaaattg caatacggta attctttctt taaatactca
    36061 cccagtgaaa acagaggata gtgttgagga gcaagtacac aaatatcttt ttcatgaatg
    36121 ccgctttcaa tcttttcctt tattatattt tttattatgt tgggtaaatc ctccttagta
    36181 actttgcaat cataacttat aaaaccacgg tcgtttttat tattccctct gctttcaatc
    36241 tcgtaaggac aactctgaaa attagagtag aagtctacta atctctgatt agacctgtaa
    36301 cagccatgta gcgtctttga ttgaaaagtg tgactagtta gcatttctag ttcttcaact
    36361 gatttagcta taccatctaa tgatccataa attgcttggt caatatctcc aacaaacatt
    36421 gactgaattt gagggttgtt ttgaagtaat ttagctatta cattgtattg taattctcta
    36481 gtatcttgaa attcatccac ataaatcgcc cttatattac tagcaatatt acgtgcaacg
    36541 ttactgttta cagttaaaat tttatacgct actagtaaaa tcatatcaaa atcaatttct
    36601 ttattttttt ccaacatagt gtgatacatt tcagaaactt gagtatactg aacttcatta
    36661 attttaaaat catgagaaag tgacaaatca attttgttca gttcatacgt tttgagattt
    36721 aaattcagca tattattaat ttggctgacg taagctcttt gagtataatc atcaataatt
    36781 gtaaatcctt ttgatactct tgagagattc attttaaaag gataaataat aaattcaaga
    36841 caaaactgat gtattgtccc cacccaaata ttaatatcat catctatact aagtaaactt
    36901 aggcgttcct taatttcttc agcagctcta ttagtgtatg tgatagcaat tattcttttt
    36961 agtgatccat cgtgtttaat tgcttggtaa gcaattttcc ttgtaagtac ccttgttttc
    37021 ccactcccag gacatgcatt tacataaagg ttatcttcac tttccacaac atctttttgt
    37081 tctaaattta aatcatttac gattttttcg aagatatttg tcattttgaa acctcagcat
    37141 aaagtatttt tgtgtaattt tctatctgat tgattagata cacaacatcc tcatctttac
    37201 caaagtactc taataaagaa attattgcta tattcaaatc agctgtatca cttgttttta
    37261 aactagctga taaagctgac aattctgtag tttcctcgta caagtcgatt gtatggagta
    37321 gaattttttt ataaattgag agatctacta tttcctgaca tgcaaaagca atagcattaa
    37381 gaatatactt aggaattttg attaaaaagt caattttttc acctaactca attgcaaacc
    37441 accccttacc aacatgattg gctagtttta acatcgctaa atttctttct aaatattgtt
    37501 cactttgtat ttcttcttta taagcagtta ttgttttttt atctttgaat gcgtcatcta
    37561 taattggact gacataagtt agaatatttt ctttattcac atctaaaaac tcaatttcaa
    37621 atgttgtatt agcataaaaa caattaaccc aattgttttc attaaaaagg gaggataatt
    37681 tttcttttct tgcttgacct ttctcttgtg cagtgctttt atacaatcga tgttctgatg
    37741 ggattgcctg ttcatctaaa tcagttacaa tagcacactt tctctgtatt ctatcatcgt
    37801 caaacaagct agcgatatat tcaaacgaag tactaccaac attaatcaaa cctattccaa
    37861 gttcgtctaa gtttatacca aataattttt ttactatttg aggaataagt atttcttcag
    37921 catctccttc aactaagata acacctttag aaaaaagcaa tccatttctt ttaacatcta
    37981 aatatctctc tactgcgttt attagattta gattgttctt ttttaacttt tctttagcaa
    38041 atgagttcag attatttgct ggattcataa cgatagattt gccattaaac gattttaata
    38101 cattcatccg agatatttca gacacttcag ctaaatgtac agaatgtgtg gtcatgagaa
    38161 tctgagtata gtttttttga agatttaatc cttcgaacag agttttttga atatgatgat
    38221 gaatatgagc ttctggttct tcaatcaaca taatattaag tagttctctt gaacgacacg
    38281 cttcaaattc gactattttc aaagctaaat aaatcatatt tagatgtcct aaccctagta
    38341 agtctaagtc gatattttgt tctggcttca ttgaaagaaa ttttgctaaa gaacccatat
    38401 catctgacat agttgaggaa agaagaagat taggagaata cactgaacca attatgctat
    38461 ttagttgccg attaatattt tcgccaattt tcctaatttc aggaacactg gtaatagtct
    38521 catttaattg ctgtataatt gtttttaatg actcaacatt atctgatgat atttttgatt
    38581 caattgtttc catgattctt ttgactgggt ttcgattgtt tttcatttct ctaagaacat
    38641 ctcgaagagc gtcaacataa acaaccgaaa tatgcttgaa aatatctgac atgtcaattc
    38701 ttgagcccaa cagagcttca tcgtcttctt caggattact agctatattg tcattgagtc
    38761 ttcctaccaa tttatcataa ttttcattga tacagaaatt tagatttgat ttagatgtgt
    38821 agtaaaattc atagtcggat agacgaatag aatttctaaa ttcgttaaat tgatgagagt
    38881 ctaattcagt ggaaatatca aataattgct ttctaatagc tttgttaggt ctaataaata
    38941 gagaaatact acctacacct ttatctgcat ttgaaatcaa ttcttctaag attgtttgac
    39001 tttcagaatt taaagaaatg gaagcacaaa tctcattgtc aaattcttct tcgctgattc
    39061 cttcaaatgt cgcagagata ataatccaat gcccttgcca cccattttga attccatgaa
    39121 aaaaatctga ttcttttagt gttttactac tatagtaata actatcatct aataagattc
    39181 tcaatgcagt aagagcattg gttttccctg aatcattctc acctataata gtatttgtct
    39241 cattatcgaa tcgaaagatt tcggattcat aatttctaaa atttcgaatt tgaagagtag
    39301 atataaacat aagcagatct cctaactatt taattaatat gattatatag taagtttatc
    39361 ataaattatg ttagtaaaag ttctcattat aagggagcgt caataatttt gtgtaaatga
    39421 ttcttcccta ctgcagattg tttctaattc tcaatcagtt ggcttagcat ttgttcgagc
    39481 tcgccttcgg cctgttggaa acctttatga ctgcgaccta ggaatttttg gttgtaagta
    39541 tcaaaagacg agactaagaa acgttccatc gactcttcat tttggaactg ttccttgcgg
    39601 tggctgtatt tcttgatttg tttattaaag gattcaatca agttcgtaga ataaatactc
    39661 ttgcgtatgg ccaaggggaa atcatagaaa gtgagcaagt gatcgttcga aagaatcgat
    39721 tcaaccactt ttggatagct ggttttccat ttttccgcaa aagtaccacg tgcttccaac
    39781 gccacctctt tagatgaggc ttgatacacc attttaaaat catcacagac ttccttacga
    39841 tcatcgacac gcactttatg actgatatta cgggaaacgt gtacacaaca atgttgaaaa
    39901 cgagctttgg gatagaaccg actaatcgct cctaccatcc cctttaagcc atccgtgatg
    39961 aacaggagga catttttcaa accgcgctct tgaaggtcca ataaaatttc ctcccaaatc
    40021 gtgatggatt cagtcggtgc aatcgcatag ctcaatactt cctttgatcc gtccgggcga
    40081 atgccaactg cgatatgaat agcttccttg gcgacggttt tccgctttaa cggaatatac
    40141 gttgcgtcca tataaatagc agcataacgg tcatgaagct cccgcccatt aaacgccgtt
    40201 acctcttctg taaatgattt tgttatattg gacatggttt ggggcgtgta gtaatgccca
    40261 tacattttct caatcaagtc tgcgatttcc gacatggtaa taccttttcg gaagaggtga
    40321 atgacggtct cctctaacgt gtcattcgtc cgtctataag caggaacagt ctgttgcttg
    40381 aactcaccat tgcggtcgcg cggaatctgg agatgaagtt ccccatactc ggtcttgacc
    40441 gtacggtcat aggagccgtt acgagaatta cccgtgttaa aaccaatgcg atcatacttt
    40501 tcgtaatcta ggaaagccgt taactccgtt ttgagaagcg tattgactgc tttctccaaa
    40561 tggacacgga ataattcatt caaatcgcct ttgttcgcta gagtctttag aatttctgta
    40621 gtaaaatcgt tcatagggaa gtcctctctt ctgtgaatgt gttgtggtaa ctctattcta
    40681 cagaagggac ttcctttttt ctatttacac aaaatatttt acactctcca ttataacaat
    40741 tgggttaaaa agaaaataga tagtattcta cctcatattt atttgaaagt aatatataaa
    40801 agacgttcaa attcgaacgc ctctcgttaa ccaaatattg gattcagaat ctagttttca
    40861 tatataaaca gagtatgatt aggtgcaaaa attgcacttt tattgcactt ttatttttat
    40921 ttatagtgtt ttactgttct gtatattgtt ttaactccct tgatagtatc gcattctgtt
    40981 ttatacactt tacgttgaaa acagagtggg aataaagagc caacataata tggaggggga
    41041 atccctataa atcaacattc ttcaatagct aaaatgcttg attttcatta aaaataagat
    41101 ggttccctac caaaagagac tataatataa gttaatttgt ttcattctta tcataaaaag
    41161 aagttctcga aaaacgaact gttttccgag aacttttttc tatccatcta agtgctcaaa
    41221 tcatattcac gacggaattc taaggttacc ctttcttaaa caaactttat gttatctgca
    41281 caactttttg caattttttc attgcttctt acgaaatcgt tgaactctct ttagcataat
    41341 acaaagtgta gatgaagtct ggtgctttac tcgttttggt aaaaatgact ggaaatcgtt
    41401 tctggttatt tttggcaaag gcgaggtgca ggttcgattc ctgccttcta cgctaacgat
    41461 gtggctgaag ggaacctccg atcttgaatc gaaacgatcc gcagtctatt gtttcaccaa
    41521 tgaaacaaaa tcaacgttcg tccaagcatt tcctgaagcc ttgctataaa ccccaatttc
    41581 acaaacacct gttgtaattg gaatgtcttt gattgtaatt tttgtccatt cacttgtttt
    41641 aacaaccgca gtatttagtt ccgctccacc ataatttttg atatataact gattagtatc
    41701 aaactctccc ccagacatta cataggcact caatgtgtaa gttcctttgc ttatgccact
    41761 aacattttga taagctgatg cctcgtatgc ttgatcctgc caaaaagtta gtttgtagtc
    41821 cccaaaaaaa gtgcccgctt ccgttttgat cgtaccctta gcggttcctt gttgaaacca
    41881 ggtttgccaa ttggaaggac tggtcgttgg tccatcgctt tcgaaacttg catttttaac
    41941 tagatttatt tgcgcccctg cttgtttaaa cgcacgcaag cttggcaagg cgtttccagc
    42001 aaaatcaaac atcgcttggt tgtcccattc gtttcccata gtggattgaa cactgagata
    42061 attcatacct gcttgtgttg cccaactcac atttccgttg taccaaagag gttcccaata
    42121 gaagaagccc gccccgcgat tattcggtac tgcctgaatc cgatcggcaa gatccagcaa
    42181 atactctgct tgtcctgtcg gtgttggttc atatcccccc gcagctgctt cggcttcacc
    42241 aaaagcattt ggcatagtat cggcattcgc tgttgtatgc ccataagctg tttcaactac
    42301 gatcacttct ttaccatacc gttggctgat cgcattcata ttggtttgaa gttcattcat
    42361 tgaaccgtgc caatacggat aatacgacac tccaattaaa tcaaatgaca cacctcgact
    42421 cgtgatttca tcaaaccacc aagtaaatgc tcctgtatcg ccaccatcag ctaagtggag
    42481 cattactgga gttgttttag gttgagtgtc tcgaacagct tggataccag acttcaaaaa
    42541 agcagccaat cgatcaaatt ctcccccgcc ttctccccaa gacttgccat aaggccagag
    42601 cattccgcta ttcagttcat tgccgatctg aaccatatca ggataaacgt ccagctgctt
    42661 catttccgac agaacgttct ttgtatagtc gtacaatgcc gtattcattt cttcaaaggt
    42721 cagaccttgc catgctttag gaagattctg tttcccagga tcaacccaaa aatcactgta
    42781 gtgaaaatcc agcagcacct taagtcccag attttttgct cgttttgcaa ggtcaatcgt
    42841 cgtttccaga tcatttgtcc ctgctccata actttctcca ttactattat aaggatcctg
    42901 ccacaaacgc agtcgaacat agttgacacc attatttttt aaaatctcta aggcatcttt
    42961 tgaaatatcg tcttcataat attttgcacc agatttttcc atatctgaca ggatcgaaat
    43021 atctgctccg cgaataaatt cgctggcaga tgcttcaaca gtttttgaaa cagcccccaa
    43081 agcccctaac acagctgcaa ataaaacagc tttacgcaac caaattccca ttttcattat
    43141 ggctcctcct ttattgaaat tggttcagaa taaactgaac agctttttca ggatctctcg
    43201 ttaatccgat atattctgct ccttttgtcg caaccagctt gattcctaag cgatcggtgt
    43261 cttctttgat atcatcatag taagaattga cttgcggtgc taagaccacc atatcaaaat
    43321 cctgcagcat ttcgtaatga gaaccataag cacccgccat tgcactgatc ggtgctttcg
    43381 attcagatgc accttttttt aaagcattag ctaacatcgc acttgtccct gcgccagcac
    43441 aaagtaccaa cacattgatt tctttatttg catcgctgat aacttgactc gctgccacag
    43501 gtacttcctc acgtacatcg gcagaagcac cttcctcggt tgcgatcacc tctgcttttt
    43561 gtgcttcttt ctctactaag acagcatcgt atgctttgca aaatggtaga tagatcagga
    43621 agtcaacgac gattagaacc gctgctagaa caaaagctaa gatacccatg cccgttccta
    43681 gaaccaaacc gatcggtgct ggagttgccc aaggcaaaac gtacataaag ctgttcatgc
    43741 ccaatacatc gacaaaaaac ttgaacaggc aaacgtttac aacaggtgtt aacaaaaatg
    43801 gaataaaaaa ataagggttc aaaatcatgg gtgccgcaaa tagcaatggt tcgttgacag
    43861 cgaaacttac tggaataaag gaagctttcc caactgcctt caactgctta gacttagcaa
    43921 agagtaggaa gataaaagga acaaccaatg ttgccccagt tcctcccata gttcctacaa
    43981 aattccctaa acccaccgtc agtactttag aagcttgttc accggaattg aacaattgca
    44041 gattggtttc cacattggca tagataattg ccgcaattgc cggctccacg atcgacggtc
    44101 catgaacacc gacaaaccaa aacagcgcca tcgctcccca gataatagcg attcctaagt
    44161 acccgtcagc tgctgtaaat agcggctgca gcaccgtaat gattgcttct gcaaatgaga
    44221 tttggaagat ttgtcgaatg cctaaatcaa ttaaaacggc cgcaaatact gaaaaagaga
    44281 aaggaaaaat atctcggaag gtttgcgaaa tcgttccagg aacttcttta ggcattttaa
    44341 tggtaatatc tttcagcaca caaaacttat ataaattaac tgttaaaaat gcagcgacaa
    44401 aggaagccag tagtcccttt gtccccatat attccgttga aaatccacct tcaatcggag
    44461 agacgcttaa caacaggaaa cttaccacag aggccagcat cacagaggtg gtgttgatta
    44521 ctttgttttt cggtaagcga cgattcatcg aatcagccaa gcagcgagca gtggttgctg
    44581 aaacgagtaa ccctacaaca cccatcgaat aattatagac tttccatagc catgtggaga
    44641 cagattcagg ccattggtaa ccaaaaatct caggaaccgc agcaatcaaa atgaaaatgc
    44701 ttgaaaataa aatagctggc atcgctgtta aaaatccgtc acgaacggct cctaaataac
    44761 tattacgtga gattttttca aaaaatggtt tccctttttc gatttgttta atgattccat
    44821 ccatagcgtt cttctcctat ccttgattct gtctgtacag tacaataaag tcttgaatca
    44881 gatctctcag caataaagtg gtcattaaat gatcctgccc atggataaaa atgaatccca
    44941 tctccatctc tttcccagat gcttcttccg ctaaaatttt tgtttgcgaa ttatgtgcaa
    45001 gggttaaatt ttcgtcggct tctttcaacg ccgaatcaac atgttcaaag tttccttgtc
    45061 ttacttcttt caataatttc aataatgtac tgcgagcgtc gcctgaatat gcaacgatct
    45121 caaaaccaag catttgtaat tcctctttat tcatcttttc ttcctccatt atcacttatt
    45181 aaataatcgt tttggtttca ctgaccaact tgtaccagta agctgattct tttgggtaac
    45241 gcttctgggt ttcaaaatca acataaaaca agccatacct tttgttatac ccatttgtcc
    45301 atgagaaaag atccataagc gaccacaaaa aatacccctt cacatttacc ccggctgtta
    45361 tcgccttgct taaggattcc aaatagactc tcaaatagtc gattctcggc tgatccataa
    45421 taatgccatc ttcaaactga tctttatacc ccatcccgtt ctcagtgata tagattttgt
    45481 tgtaatgtgg gtaatcgctt ttaattcgca gcaacaagtc atatagacct tctggataaa
    45541 tcagccagtc ccaatcagtt cgaggaattc cttctttata aatacgttct ccgattccct
    45601 tcactttata aacagaggtc cctttttcac ccgtaccgtt atgatggatt gcattttctc
    45661 catcgtaagc tttgacaaaa tggcattggt agtgattgat ccctaagtag tcattacgcg
    45721 tggatgcttt tttcaattca acgaaatctt cttcaggaaa atgatagctg gcctgatttg
    45781 cttcacatat ctcatcaaga gcagtcaagg tttcagttga ataatagcct aaataggtag
    45841 catctaataa gaaacggatc gacaatgcat cgtctaaaaa ggcagcatgc ttatcttccg
    45901 gcgcgtctgt tgccgcatat ttcgtctcta atgagtgaac tacaccaatt tcacccggaa
    45961 gttcgttttc tttgaaatag ttcactacac gggcatgagc aaccatcata ttatgaagac
    46021 aggcgacgat ctttgtaaaa tcatatttga ttcctggtgg gaaaacacct aacaaatatt
    46081 gattggttgc aactggatag atttcattaa aggtactcca caccttgact tctttgaatt
    46141 catgaaaaca aaaaatggca taggaaacaa atgcttctat tgtttctcga ttcaaaaaat
    46201 ctccatggtc aaacaaacgt ttaggggtat caaaatgatg caacgtcaca aacggcgtta
    46261 cgtttctttt cttacattct tcgaacacac gatgataaaa agcgattcct tctggatttg
    46321 gtttcccagc tccatcagga aaaatgcggc tccaagcaat ggataatctt agaccattta
    46381 caccaaaacg ttcacacaat tcaatgtcct ttgcatattg atgataaaaa tcacttgccg
    46441 gatcgggact gaatcgccct tgttcctcta aaaagtcatc ccaagcaaca gcgcctttgc
    46501 caccttcctt agtggctcct tcaacctgat aggctgctgt ggctccgcca aaaataaaat
    46561 cttctggtaa tttaagcatg tacatctctc ctcaaatcta taatcagttt tcgctctttc
    46621 taactgattg cattgtaaac gcatccaaaa catttgtcaa caaaaacaaa cacaaataat
    46681 cataaaaacc agaaaacagc aataaaatct attgtaacaa acataaaaac caccctaaat
    46741 gtttactttt gttcgaatta gattgactgt tgtccgttta tatgatagat tatccttaaa
    46801 ttagaggtga agacaatgtt gaaaaaagaa agattgctta ctatcgtaga aatggtcaat
    46861 aaaaaaggaa tccttaccgt taatgaaatc atcaatcaat tagatgtttc tgatatgacc
    46921 gttcgcagag atttagatga attagaaaaa tcgggcaagc tgctgcgtgt ccatggaggg
    46981 gctcaaagtc tttcctacac tttagatcaa gaactttctc acactgaaaa agccactctg
    47041 cagattcaag aaaaaaaaga aattgctgcc acagcagcct ccttgattca cgagggagaa
    47101 acgattttct taggaccagg tacaacaatc gaactattgg cgacgcattt actgaacaag
    47161 aaaattcgga tcattacaaa taattatcca gtttttgata ttcttgctca agcagatgga
    47221 ccagaaattc tactgattgg cggtgatttc cggaaaaata ccggcgcatt tgtcggaccg
    47281 atcaccaacg acaatttaag aagactaaag tttacaaaag catttatcag ttcaaatggc
    47341 gttcacaacg aagcgatctc tacttacagt accgaagaag gagaagcaca gcagatcgct
    47401 ctaaataatt cccgtaagaa atatctactc gttgataata aaaaattcaa tcgagatgat
    47461 ttttatgtgt tttataattt gcatgatttc gatctgctca ttacggatcg ccagatctcg
    47521 cgggaggttc aagagcacta tgcacaatat gtcgatctgc aaattgctga ttcaaccgaa
    47581 aataccacta taggagcgta actatgaaag ggattatatt tgatttcaac ggaacaatgt
    47641 ttttggactc tcatctgcat gaagctgctt ggctgcatat gatacatgat catacaaaag
    47701 atggtttatc agacgaggat attttaaaaa atattcatgg tcggacgaat acggagattt
    47761 tgaaacattt catttcagag catttgaccg cctctgagat cgccgtattg tcagaagaaa
    47821 aagaaagcta ttatcgtatg ctttgcttgc aaaatgaaaa cgagcttcag ttgacaaaag
    47881 ggcttgaagc gactcttgac cagctgactg accaacagat tccattaacg attgccacgg
    47941 ctactgtcaa agagaatgtt gctttttatt ttgatatttt cgatttagcc cgctggtttg
    48001 actgggacaa gattgtctat gacgacggct ctttccctgg gaaaccgcaa ccggatattt
    48061 ttcttaaagc ggcagacaaa ctgagcttag ctccggctga ttgtcttgtt atcgaagatg
    48121 cttactcagg attacttgct gccaaacgtg cggggatcgg aacaattatt gcaattgatc
    48181 cattcggtaa aaacaaaaca gtatttgaac aggcacagct aaataaagat ggaaccatca
    48241 aagattttac tggtttttgg acagagcacc tgtctgccac atccgttaat tcttaaacga
    48301 ttcaactaac taccaaagaa aagcaactga aatgcacacc tccttttata aaaaactgcc
    48361 agtgaaacga catgaaagat acactgctat atccttagtg aaatatagca gtgtatcttt
    48421 gactttttca ttcttttaac catatgacgc gttcatctct atctataatt aacattaaaa
    48481 atagtgtttt cacattattt ctcaaaccaa tcttaaaaat catcaggagt acttttttga
    48541 tatactttta ttaatgctgg gcgtaatggt taaaagattt ccactagttc ctctccacca
    48601 agctgagttg aaaaatgctt atctgtaaac aacatagaca aagaggaaat aaacgtttcc
    48661 gtatctttct ctgtcccaag gatattaatt aattttccta ctaggtaagt atcattaaac
    48721 ttaagatctg ccttcacatc aaaagttttg ataaaagctt ttatcatgcg tacaattttg
    48781 tctttatcgc cataattgat ttcaccactc atatcaatat agtcactaat ccctgaagaa
    48841 ataatggcaa ctgcttcttc tttcgcctta tcagataatt catttaatga ctgggattga
    48901 ttgatgactt cttgagtttt atctgttacg taatctctca ttatcctatc ggcatactcc
    48961 gcaaagtaat cttgaaaaaa ttcttctaca gggtaaccaa cttcgttttc ccaaccagga
    49021 gaaatttcat tatcagtact tattaaatct attaaaaacc aacccgttct ctgagtgttg
    49081 attctataat attttta
//
LOCUS       DACEND010000007        45836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEND010000007 DACEND010000000
VERSION     DACEND010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.007"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            508..1698
                     /locus_tag="IW746_000330"
     CDS             508..1698
                     /locus_tag="IW746_000330"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1077261.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1800..2753
                     /locus_tag="IW746_000331"
     CDS             1800..2753
                     /locus_tag="IW746_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1077262.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2822..4171
                     /locus_tag="IW746_000332"
     CDS             2822..4171
                     /locus_tag="IW746_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1077263.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4354..4677)
                     /locus_tag="IW746_000333"
     CDS             complement(4354..4677)
                     /locus_tag="IW746_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077264.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4806..5969
                     /locus_tag="IW746_000334"
     CDS             4806..5969
                     /locus_tag="IW746_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077265.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            6039..7388
                     /locus_tag="IW746_000335"
     CDS             6039..7388
                     /locus_tag="IW746_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1077266.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7433..7864)
                     /locus_tag="IW746_000336"
     CDS             complement(7433..7864)
                     /locus_tag="IW746_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077267.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8252..10894
                     /gene="alaS"
                     /locus_tag="IW746_000337"
     CDS             8252..10894
                     /gene="alaS"
                     /locus_tag="IW746_000337"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1077268.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11094..11690
                     /locus_tag="IW746_000338"
     CDS             11094..11690
                     /locus_tag="IW746_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1077269.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11812..12960
                     /locus_tag="IW746_000339"
     CDS             11812..12960
                     /locus_tag="IW746_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1077270.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12998..13411)
                     /locus_tag="IW746_000340"
     CDS             complement(12998..13411)
                     /locus_tag="IW746_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1077271.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13490..13921
                     /locus_tag="IW746_000341"
     CDS             13490..13921
                     /locus_tag="IW746_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1077272.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(14020..14253)
                     /locus_tag="IW746_000342"
     CDS             complement(14020..14253)
                     /locus_tag="IW746_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1077273.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14436..16106
                     /locus_tag="IW746_000343"
     CDS             14436..16106
                     /locus_tag="IW746_000343"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1077274.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16253..16639
                     /locus_tag="IW746_000344"
     CDS             16253..16639
                     /locus_tag="IW746_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077275.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16626..17324
                     /locus_tag="IW746_000345"
     CDS             16626..17324
                     /locus_tag="IW746_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1077276.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17329..17793
                     /locus_tag="IW746_000346"
     CDS             17329..17793
                     /locus_tag="IW746_000346"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1077277.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17790..18701
                     /locus_tag="IW746_000347"
     CDS             17790..18701
                     /locus_tag="IW746_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1077278.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18936..19475
                     /gene="pyrR"
                     /locus_tag="IW746_000348"
     CDS             18936..19475
                     /gene="pyrR"
                     /locus_tag="IW746_000348"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1077279.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19489..20772
                     /locus_tag="IW746_000349"
     CDS             19489..20772
                     /locus_tag="IW746_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1077280.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20785..21711
                     /locus_tag="IW746_000350"
     CDS             20785..21711
                     /locus_tag="IW746_000350"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1077281.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21730..23010
                     /locus_tag="IW746_000351"
     CDS             21730..23010
                     /locus_tag="IW746_000351"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1077282.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            23015..24091
                     /gene="carA"
                     /locus_tag="IW746_000352"
     CDS             23015..24091
                     /gene="carA"
                     /locus_tag="IW746_000352"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1077283.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24096..27281
                     /gene="carB"
                     /locus_tag="IW746_000353"
     CDS             24096..27281
                     /gene="carB"
                     /locus_tag="IW746_000353"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1077284.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27335..28111
                     /locus_tag="IW746_000354"
     CDS             27335..28111
                     /locus_tag="IW746_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1077285.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28108..29040
                     /locus_tag="IW746_000355"
     CDS             28108..29040
                     /locus_tag="IW746_000355"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1077286.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            29037..29753
                     /gene="pyrF"
                     /locus_tag="IW746_000356"
     CDS             29037..29753
                     /gene="pyrF"
                     /locus_tag="IW746_000356"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1077287.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29760..30389
                     /locus_tag="IW746_000357"
     CDS             29760..30389
                     /locus_tag="IW746_000357"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1077288.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30377..30691
                     /locus_tag="IW746_000358"
     CDS             30377..30691
                     /locus_tag="IW746_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077289.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30729..31370
                     /locus_tag="IW746_000359"
     CDS             30729..31370
                     /locus_tag="IW746_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1077290.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31401..32105
                     /locus_tag="IW746_000360"
     CDS             31401..32105
                     /locus_tag="IW746_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1077291.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            32102..33004
                     /locus_tag="IW746_000361"
     CDS             32102..33004
                     /locus_tag="IW746_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1077292.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33162..34868)
                     /locus_tag="IW746_000362"
     CDS             complement(33162..34868)
                     /locus_tag="IW746_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1077293.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            35058..35681
                     /locus_tag="IW746_000363"
     CDS             35058..35681
                     /locus_tag="IW746_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077294.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36131..37180
                     /locus_tag="IW746_000364"
     CDS             36131..37180
                     /locus_tag="IW746_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077295.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37177..38058
                     /locus_tag="IW746_000365"
     CDS             37177..38058
                     /locus_tag="IW746_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077296.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38061..38831
                     /locus_tag="IW746_000366"
     CDS             38061..38831
                     /locus_tag="IW746_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1077297.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39110..39964
                     /gene="aroE"
                     /locus_tag="IW746_000367"
     CDS             39110..39964
                     /gene="aroE"
                     /locus_tag="IW746_000367"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1077298.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40142..41305
                     /locus_tag="IW746_000368"
     CDS             40142..41305
                     /locus_tag="IW746_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1077299.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41422..41832
                     /locus_tag="IW746_000369"
     CDS             41422..41832
                     /locus_tag="IW746_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1077300.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            42006..44234
                     /gene="topB"
                     /locus_tag="IW746_000370"
     CDS             42006..44234
                     /gene="topB"
                     /locus_tag="IW746_000370"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1077301.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44372..44689
                     /locus_tag="IW746_000371"
     CDS             44372..44689
                     /locus_tag="IW746_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077302.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            44849..45085
                     /locus_tag="IW746_000372"
     CDS             44849..45085
                     /locus_tag="IW746_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1077303.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45082..45480
                     /locus_tag="IW746_000373"
     CDS             45082..45480
                     /locus_tag="IW746_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1077304.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        15045 a         7921 c         9924 g        12946 t
ORIGIN      
        1 agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt
       61 ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac
      121 aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat
      181 ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa
      241 ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag
      301 taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa
      361 aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca
      421 aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca
      481 agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag
      541 aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt
      601 gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat
      661 tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact
      721 tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa
      781 ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga
      841 ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg
      901 gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc
      961 cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt
     1021 aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt
     1081 gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat
     1141 gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta
     1201 agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt
     1261 tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta
     1321 ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg
     1381 caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa
     1441 ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg
     1501 ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct
     1561 aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact
     1621 gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa
     1681 gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt
     1741 tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca
     1801 tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc
     1861 tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca
     1921 ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca
     1981 tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg
     2041 cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg
     2101 aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc
     2161 caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg
     2221 gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag
     2281 atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta
     2341 cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt
     2401 tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta
     2461 taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg
     2521 cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg
     2581 aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac
     2641 tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga
     2701 tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga
     2761 accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg
     2821 gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg
     2881 tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt
     2941 attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc
     3001 ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc
     3061 tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc
     3121 agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt
     3181 gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt
     3241 tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc
     3301 tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt
     3361 cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg
     3421 agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata
     3481 cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt
     3541 gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc
     3601 ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga
     3661 cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat
     3721 ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat
     3781 ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa
     3841 agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg
     3901 tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt
     3961 ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt
     4021 tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag
     4081 cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat
     4141 cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc
     4201 acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa
     4261 gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa
     4321 agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat
     4381 tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag
     4441 ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc
     4501 catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat
     4561 aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt
     4621 agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc
     4681 gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt
     4741 tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga
     4801 gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag
     4861 ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag
     4921 taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg
     4981 tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc
     5041 tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac
     5101 ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt
     5161 tattactcat accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa
     5221 cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt
     5281 attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat
     5341 tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa
     5401 gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg
     5461 tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct
     5521 ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag
     5581 taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa
     5641 ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga
     5701 tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc
     5761 agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc
     5821 tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa
     5881 aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc
     5941 aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat
     6001 gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc
     6061 gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg
     6121 tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa
     6181 ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa
     6241 aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca cagatctatg
     6301 aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg
     6361 gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag
     6421 taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg
     6481 catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag
     6541 tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta
     6601 ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa
     6661 tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta
     6721 aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg
     6781 tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct
     6841 tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac
     6901 aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg
     6961 atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta
     7021 tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt
     7081 cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa
     7141 aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag
     7201 aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa
     7261 agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag
     7321 aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac
     7381 ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta
     7441 gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat
     7501 gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct
     7561 gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt
     7621 ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct
     7681 tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt
     7741 gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa
     7801 cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt
     7861 tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt
     7921 accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt
     7981 gatcaataga atgcgtatac taaataagta cgaaaaggat atgatttgtt ttttaagact
     8041 cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt
     8101 ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac
     8161 acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt
     8221 tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg
     8281 acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt
     8341 gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa
     8401 atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat
     8461 tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat
     8521 gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga
     8581 atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta
     8641 tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca
     8701 cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac
     8761 ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa
     8821 ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt
     8881 caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat
     8941 gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt
     9001 gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa
     9061 cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc
     9121 tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct
     9181 tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt
     9241 agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga
     9301 atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg
     9361 tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc
     9421 aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga
     9481 ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc
     9541 acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc
     9601 tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga
     9661 tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga
     9721 agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga
     9781 tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa
     9841 agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca
     9901 aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc
     9961 aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg
    10021 ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc
    10081 agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt
    10141 tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg
    10201 tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg
    10261 cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc
    10321 aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact
    10381 acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt
    10441 gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga
    10501 aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc
    10561 agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca
    10621 gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc
    10681 aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc
    10741 tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg
    10801 tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact
    10861 taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa
    10921 aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca
    10981 tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt
    11041 gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata
    11101 gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac
    11161 aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta acaattatcc
    11221 atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct
    11281 tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata
    11341 cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag
    11401 caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct
    11461 ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat
    11521 tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct
    11581 tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac
    11641 cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt
    11701 ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt
    11761 cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag
    11821 aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg
    11881 ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt
    11941 ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca
    12001 tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct
    12061 tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat
    12121 gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct
    12181 ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc
    12241 aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca
    12301 gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg
    12361 gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat
    12421 agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca
    12481 gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat
    12541 gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta
    12601 gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt
    12661 aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa
    12721 aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt
    12781 aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc
    12841 cctgagaaat tccagtttgt tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa
    12901 gaaaaaatcg gagattacca attggaaatt acgtttgatc acaattatct tttatattaa
    12961 atgagaaact ataaaaagac tccttaaagt ttttaattta aagagtcttt ttttctttta
    13021 attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca
    13081 accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg
    13141 gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt
    13201 ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt
    13261 ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct
    13321 gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt
    13381 ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact
    13441 aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa
    13501 tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat
    13561 tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt
    13621 tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa
    13681 atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg
    13741 acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa
    13801 agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga
    13861 aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta
    13921 attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt
    13981 acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc
    14041 ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat
    14101 gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc
    14161 agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt
    14221 gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga
    14281 tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat
    14341 tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat
    14401 tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac
    14461 aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg
    14521 atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa
    14581 aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg
    14641 aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat
    14701 ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg
    14761 caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg
    14821 acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc
    14881 atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc
    14941 tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc
    15001 caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg
    15061 cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg
    15121 accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga
    15181 aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg
    15241 ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat
    15301 tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga
    15361 aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg
    15421 ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga
    15481 atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac
    15541 aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac
    15601 tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat
    15661 gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata
    15721 gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag
    15781 aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa
    15841 aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa
    15901 cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca
    15961 cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg actggagatg
    16021 ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg
    16081 acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat
    16141 tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat
    16201 tctttttctt tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac
    16261 gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa
    16321 caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc
    16381 ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc
    16441 aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt
    16501 tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt
    16561 tattgtgttg attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga
    16621 aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga
    16681 gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc
    16741 aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa
    16801 ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt
    16861 ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc
    16921 ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa
    16981 agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag
    17041 cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg
    17101 ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt
    17161 atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag
    17221 ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga
    17281 tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt
    17341 tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact
    17401 gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat
    17461 attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata
    17521 gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt
    17581 aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc
    17641 gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg
    17701 atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt
    17761 attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg
    17821 aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa
    17881 ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt
    17941 acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg
    18001 tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa
    18061 acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca
    18121 atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta
    18181 ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg
    18241 cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt
    18301 tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga
    18361 aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta
    18421 ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa
    18481 cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat
    18541 acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt
    18601 ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg
    18661 aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta
    18721 attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa
    18781 gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta
    18841 tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag
    18901 gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc
    18961 aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata
    19021 gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa
    19081 ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca
    19141 ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca
    19201 tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac
    19261 gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat
    19321 tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga
    19381 agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac
    19441 aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa
    19501 aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat
    19561 tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc
    19621 atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg
    19681 tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt
    19741 gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc
    19801 acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg
    19861 ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga
    19921 cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat
    19981 attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt
    20041 tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg
    20101 tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt
    20161 gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca
    20221 cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta
    20281 actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta
    20341 gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt
    20401 ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt
    20461 gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca
    20521 gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt
    20581 ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc
    20641 ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg
    20701 gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc
    20761 gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat
    20821 ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag
    20881 gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc
    20941 tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg
    21001 ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac
    21061 gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat
    21121 gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga
    21181 ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa
    21241 gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc
    21301 gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt
    21361 cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa
    21421 ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga
    21481 aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg
    21541 aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa
    21601 ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga
    21661 gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag
    21721 gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg
    21781 acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca
    21841 gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg
    21901 gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg
    21961 actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct
    22021 gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt
    22081 gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat
    22141 caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa
    22201 acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta
    22261 gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca
    22321 gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat
    22381 ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa
    22441 agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca
    22501 ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg
    22561 aacccgccgc taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt
    22621 acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt
    22681 ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact
    22741 cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa
    22801 ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta
    22861 gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg
    22921 gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac
    22981 ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga
    23041 tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt
    23101 ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca
    23161 aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga
    23221 atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa
    23281 ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc
    23341 aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag
    23401 tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct
    23461 agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg
    23521 tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa
    23581 aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct
    23641 atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc
    23701 aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca
    23761 ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg
    23821 attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca
    23881 tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga
    23941 agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca
    24001 gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat
    24061 ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga
    24121 aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg
    24181 caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt
    24241 ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga
    24301 tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa
    24361 ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag
    24421 aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc
    24481 gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg
    24541 tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc
    24601 gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac
    24661 gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa
    24721 gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa
    24781 tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg
    24841 tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc
    24901 tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc
    24961 cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt
    25021 tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat
    25081 taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg
    25141 cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag
    25201 aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata
    25261 tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac
    25321 ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag
    25381 atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag
    25441 cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg
    25501 aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg
    25561 gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga
    25621 tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat
    25681 tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat
    25741 cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg
    25801 aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa
    25861 tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat
    25921 attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag
    25981 gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg
    26041 ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt
    26101 tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa
    26161 gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa
    26221 gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt
    26281 acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc
    26341 ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga
    26401 tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac
    26461 ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat
    26521 taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt
    26581 atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag
    26641 gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg
    26701 gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt
    26761 cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag
    26821 aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg
    26881 gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag
    26941 aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac
    27001 atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg
    27061 aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg
    27121 tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag
    27181 aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc
    27241 gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga
    27301 caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt
    27361 tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa
    27421 agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt
    27481 acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta
    27541 tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga
    27601 tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt
    27661 atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt
    27721 cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta
    27781 tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg
    27841 tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt
    27901 tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa
    27961 tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt
    28021 ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc
    28081 gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct
    28141 ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa
    28201 tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca
    28261 aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat
    28321 gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta
    28381 gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat
    28441 gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt
    28501 tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt
    28561 caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca
    28621 aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt
    28681 acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta
    28741 gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt
    28801 caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg
    28861 gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat
    28921 ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa
    28981 ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga
    29041 atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag
    29101 actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt
    29161 tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt
    29221 tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa
    29281 agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg
    29341 caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta
    29401 tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt
    29461 ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg
    29521 tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt
    29581 gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca
    29641 caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc
    29701 agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa
    29761 tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc
    29821 caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa
    29881 tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca
    29941 aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg
    30001 ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag
    30061 atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga
    30121 tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag
    30181 aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag
    30241 caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg
    30301 aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga
    30361 aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat gaagatagag aaatccgatt
    30421 tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat
    30481 cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt
    30541 catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat
    30601 atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa
    30661 tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg
    30721 taacttttat ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa
    30781 gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag
    30841 catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga
    30901 aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca
    30961 aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa
    31021 agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc
    31081 gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata
    31141 aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt
    31201 ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg
    31261 ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag
    31321 aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg
    31381 agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag
    31441 acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc
    31501 cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc
    31561 tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt
    31621 tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag
    31681 atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat
    31741 ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc
    31801 taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa
    31861 aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt
    31921 atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc
    31981 atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga
    32041 acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc
    32101 aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga
    32161 agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga
    32221 aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga
    32281 agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac
    32341 tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt
    32401 cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg
    32461 gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat
    32521 cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg
    32581 agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca
    32641 tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg
    32701 ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga
    32761 tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac
    32821 tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt
    32881 aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc
    32941 gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag
    33001 ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg
    33061 tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt
    33121 gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata
    33181 gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca
    33241 taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc
    33301 acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt
    33361 gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca
    33421 tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg
    33481 tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt
    33541 ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct
    33601 tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca
    33661 agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc
    33721 ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga
    33781 tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt
    33841 gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc
    33901 tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt
    33961 tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc
    34021 tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata agctgttaca
    34081 gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc
    34141 gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca
    34201 accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa
    34261 ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc
    34321 tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca
    34381 acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg
    34441 atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc
    34501 attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag
    34561 tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc
    34621 aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt
    34681 tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg
    34741 ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc
    34801 cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca
    34861 aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga
    34921 agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac
    34981 ataaaaaaca gtgtttctat cgttgaaatc caaattttta ggtataataa aaaataggag
    35041 aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga gtacaagaaa
    35101 tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt cgacgtcttg
    35161 tttttctgct catcttatca ttgctgttaa cagttgcaat gtttttattg ccggaaaaaa
    35221 tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc aaattgaaac
    35281 cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata tcggtgatgt
    35341 tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat gatcctgagc
    35401 ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat gtacatgaac
    35461 tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt gatggaggaa
    35521 aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat aaggagttga
    35581 tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa gaattggata
    35641 gaacgggagt aacttcccaa aattccgaga ttgctaattg agacattggc gcgatgaaac
    35701 ggtgggtcta tatagcgcta tttttaatta tcagaacaag tgatgtttga tcttcaaaat
    35761 aacaaataaa aaattaaaaa atatagttga cgaaattcaa aaattcgatt tgacttaaat
    35821 caattcttaa ggaaagggtg acctgtaatg aacttatcaa aacgtatttc agcagaaaac
    35881 aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact ttcccataga
    35941 tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga acattgactg
    36001 ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct ttggccattg
    36061 tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt gactttgcag
    36121 gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt tagtttattt
    36181 agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca
    36241 gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg cattagatga
    36301 gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag gagaaaatct
    36361 tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa tgagtcaaca
    36421 gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg ctcaatcatt
    36481 agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc ctgtgggggc
    36541 tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt ctgatcagcc
    36601 gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg
    36661 aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag cagaagaagc
    36721 tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta atgagattgc
    36781 acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat tagataatac
    36841 cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata tccccgtcat
    36901 tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga tcaatcaata
    36961 tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc
    37021 ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc aatctcaagc
    37081 tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa tcattacaga
    37141 tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc tatttcacaa
    37201 ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat cttagacttt
    37261 cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt gacagctatt
    37321 actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg catgatagca
    37381 ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt agcagggatt
    37441 ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc aaatctttct
    37501 ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc tgattacttt
    37561 gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact gctattcttc
    37621 ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac gatggctcgg
    37681 tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc gaacggtgtc
    37741 attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt aaataaaggg
    37801 attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa
    37861 ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta tcagctattg
    37921 attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt ctccgctatc
    37981 atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt
    38041 tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc agaaaagtcg
    38101 taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt acaatcagag
    38161 aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca ctgttcaata
    38221 cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat cagctgatca
    38281 caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa gaccccaaga
    38341 tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa aaaagaggtc
    38401 aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt ttcagactat
    38461 gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt gaattatctg
    38521 gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct gagttactat
    38581 tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta atgcgattaa
    38641 caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat cgaatggaag
    38701 acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc gttcaagata
    38761 tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc tttgaagaag
    38821 tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa actcattgac
    38881 atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat ttgggtgttt
    38941 tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg ctctgacaag
    39001 taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact ttttcgtatc
    39061 aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga tgatttctgg
    39121 acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat ccccattgat
    39181 gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt tccaagtaaa
    39241 tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt taggtgtaaa
    39301 tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa gccaggaagc
    39361 gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga ttggctataa
    39421 tactgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa
    39481 aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc aagcagcgtt
    39541 agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg ctcaagtaaa
    39601 agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc gtgactttgc
    39661 cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca atgcaacaga
    39721 tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca tgatgcatga
    39781 gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat taaaagaagc
    39841 aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc aaggagcaat
    39901 cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa agctgtttga
    39961 ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc aagaaaagta
    40021 atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca aaaaatgata
    40081 ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag gaggaaccac
    40141 aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc tgaagcggga
    40201 tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga
    40261 tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc aatacgagca
    40321 acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa aatttttcaa
    40381 tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat
    40441 tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta ttggtaaata
    40501 tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc atgggcaaaa
    40561 agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg gagaagatgc
    40621 cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata agccgaaact
    40681 gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa ataagatcaa
    40741 ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag aaaattttgg
    40801 aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc
    40861 cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg atggtgtaga
    40921 tattaaaaaa gggggccaga agatggatgg acatgttctt ttgcagtacg cacgtttccg
    40981 aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat
    41041 tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag ctggtaaagc
    41101 tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc tcttttcgat
    41161 tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta
    41221 tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga atcaatctgc
    41281 tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct gcgcaaggct
    41341 tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg gtagatttaa
    41401 ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga gtcgcgtgaa
    41461 gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac cacatacggt
    41521 gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct aatcacgaat
    41581 gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga aggaattggt
    41641 cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa tgaagaagaa
    41701 acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc actggatgct
    41761 gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa ctggcagatt
    41821 aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc aaacggatca
    41881 tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt ttttttattg
    41941 ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt aacggaggtc
    42001 agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa
    42061 ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca ttgtttgctg
    42121 gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct ccaggagctt
    42181 atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca gaaaccttcc
    42241 gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga gaattgcagt
    42301 cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt
    42361 cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt ttatggatca
    42421 attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct ggaaagaact
    42481 attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg attggaatga
    42541 atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc agtttttctc
    42601 ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa gaaatcaaac
    42661 acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac aaagggcagt
    42721 tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa
    42781 tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa
    42841 aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag acaaagatga
    42901 atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa
    42961 gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat gagcatcagt
    43021 atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa gtacagacaa
    43081 ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa
    43141 ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc aaattacagc
    43201 aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca gattatatct
    43261 acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc atcggaaaga
    43321 tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc
    43381 agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa agtatggaga
    43441 aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca atgaaaactg
    43501 cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta gaaggaatag
    43561 ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat tacatcgaag
    43621 tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag gcagtagcca
    43681 atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa
    43741 ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac
    43801 atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat gagattagcg
    43861 ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga tgtccaaaat
    43921 gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat ccggaatgcg
    43981 acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat ctcagagatt
    44041 tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa aaagggaaat
    44101 tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt ccatggatcg
    44161 aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa aataatgaaa
    44221 acgaagtaga gtaaatgaaa acaatgaaat agcggaatat ttattccgaa aagtgagaat
    44281 ataaatacca ataaaactaa aaaatatcag tgaatcaagc aaaaacgttg gggtaggctg
    44341 attttatata gatagcaagg agttttttta tttgaaaaaa gtgcattcat ttttaaatta
    44401 tcttatggga gcaatcacga ttgtggctgt gtaccaagca tttctaaagt atcctgatta
    44461 cgggacgaag ttgttgtcat tgacgcacaa tcaaacagca tattcaccaa cgttgatttt
    44521 atcgactttg actgcaggag ctaaaagcgc tacttatttt tttcataaaa cgacaagcat
    44581 gctaaagcga aagaaacagc gaaaacaatt gttggtggtg gtagcggcta tgatcattat
    44641 ttattttcta ccagttatta cacaagtatt tcatcaatat tttggttaag aatacgagag
    44701 tttgtgacaa aagtcatgtc acaaactttt ttctttgtca ttttttcgta agaaaaagca
    44761 aaataaaagt atatacattg attaaaagta tatacttgga tataatgtac ttactataga
    44821 atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata aaattagaaa
    44881 atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca tgggtgtgca
    44941 gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa tgatcaaagc
    45001 acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat atgaagacgc
    45061 gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat caaaatcaac
    45121 gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga tgcaaatgca
    45181 ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact ctatcctgaa
    45241 atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc ttttcataat
    45301 ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa taattgtcca
    45361 gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac tagttcattg
    45421 gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc aataaaatga
    45481 acgaattaaa aactttattt ctcaattaga tcagcatgtc attaaggaat atgctgattt
    45541 ttttatgttt aatcatacaa aatagttttt tcataaaaat atggaaaata tggattaatt
    45601 ttgaaataat tgatttaaaa ggaatacaaa agagtgaata gtaggaaata gtggtttaga
    45661 actagtgggc tgatttttta tatattttag tttacataat atatattata caaagtagat
    45721 ttaaaaaatg ataaacctct ctcgaacggt tttttatcaa aaatcgtcat ttttccatca
    45781 aaatctgctt atgcaaaatt ataaaaaaat tttgcagttt ttcttacttt ttttgt
//
LOCUS       DACEND010000008        38991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEND010000008 DACEND010000000
VERSION     DACEND010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.008"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..763
                     /locus_tag="IW746_000374"
     CDS             62..763
                     /locus_tag="IW746_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1077305.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            760..1881
                     /locus_tag="IW746_000375"
     CDS             760..1881
                     /locus_tag="IW746_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1077306.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1896..3128
                     /gene="pepT"
                     /locus_tag="IW746_000376"
     CDS             1896..3128
                     /gene="pepT"
                     /locus_tag="IW746_000376"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1077307.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3260..4168
                     /locus_tag="IW746_000377"
     CDS             3260..4168
                     /locus_tag="IW746_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1077308.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4203..4475
                     /locus_tag="IW746_000378"
     CDS             4203..4475
                     /locus_tag="IW746_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1077309.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4486..5532
                     /locus_tag="IW746_000379"
     CDS             4486..5532
                     /locus_tag="IW746_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1077310.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5783..8392
                     /gene="clpB"
                     /locus_tag="IW746_000380"
     CDS             5783..8392
                     /gene="clpB"
                     /locus_tag="IW746_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1077311.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8543..9214)
                     /locus_tag="IW746_000381"
     CDS             complement(8543..9214)
                     /locus_tag="IW746_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077312.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9207..9701)
                     /locus_tag="IW746_000382"
     CDS             complement(9207..9701)
                     /locus_tag="IW746_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1077313.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9721..10941)
                     /locus_tag="IW746_000383"
     CDS             complement(9721..10941)
                     /locus_tag="IW746_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077314.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11088..12923
                     /gene="lepA"
                     /locus_tag="IW746_000384"
     CDS             11088..12923
                     /gene="lepA"
                     /locus_tag="IW746_000384"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1077315.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13120..14031
                     /locus_tag="IW746_000385"
     CDS             13120..14031
                     /locus_tag="IW746_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077316.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15029..15280
                     /locus_tag="IW746_000386"
     CDS             15029..15280
                     /locus_tag="IW746_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077317.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15363..15635
                     /locus_tag="IW746_000387"
     CDS             15363..15635
                     /locus_tag="IW746_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077318.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15725..15964
                     /locus_tag="IW746_000388"
     CDS             15725..15964
                     /locus_tag="IW746_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077319.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            16111..20406
                     /locus_tag="IW746_000389"
     CDS             16111..20406
                     /locus_tag="IW746_000389"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ1077320.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(20550..20918)
                     /locus_tag="IW746_000390"
     CDS             complement(20550..20918)
                     /locus_tag="IW746_000390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077321.1"
                     /translation="MFIPFKSSIENYLFQKITRKNILERKQTIQHLIQTVENKNIDFY
                     LPYELIYFAKEIQKIINTNKHSKKILFKVQRMYFEFSYCYLAELNKVRKQLGVAKRDF
                     FYRKKETVLSHNYILSLLDP"
     gene            21146..21367
                     /locus_tag="IW746_000391"
     CDS             21146..21367
                     /locus_tag="IW746_000391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077322.1"
                     /translation="MVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSIIIFFILAC
                     VWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            21324..21578
                     /locus_tag="IW746_000392"
     CDS             21324..21578
                     /locus_tag="IW746_000392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077323.1"
                     /translation="MEKELLLGFYRKENNGGKKNMEKKNINICFLSGKECTYEECTDI
                     SVHERDGMLIVDMSPSDEPFVVKHYFPLCNIESFRIDCEF"
     gene            21595..21960
                     /locus_tag="IW746_000393"
     CDS             21595..21960
                     /locus_tag="IW746_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077324.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            21962..22414
                     /locus_tag="IW746_000394"
     CDS             21962..22414
                     /locus_tag="IW746_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077325.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            22480..22836
                     /locus_tag="IW746_000395"
     CDS             22480..22836
                     /locus_tag="IW746_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077326.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            23026..24219
                     /locus_tag="IW746_000396"
                     /pseudo
     CDS             23026..24219
                     /locus_tag="IW746_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            24346..24660
                     /locus_tag="IW746_000397"
     CDS             24346..24660
                     /locus_tag="IW746_000397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077327.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            25023..26279
                     /locus_tag="IW746_000398"
     CDS             25023..26279
                     /locus_tag="IW746_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1077328.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            26276..26821
                     /locus_tag="IW746_000399"
     CDS             26276..26821
                     /locus_tag="IW746_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077329.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            26822..27322
                     /locus_tag="IW746_000400"
     CDS             26822..27322
                     /locus_tag="IW746_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077330.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            27402..27959
                     /locus_tag="IW746_000401"
     CDS             27402..27959
                     /locus_tag="IW746_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077331.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            27979..28323
                     /locus_tag="IW746_000402"
     CDS             27979..28323
                     /locus_tag="IW746_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077332.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            28342..29253
                     /locus_tag="IW746_000403"
     CDS             28342..29253
                     /locus_tag="IW746_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1077333.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            29332..30324
                     /locus_tag="IW746_000404"
     CDS             29332..30324
                     /locus_tag="IW746_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077334.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            30321..30539
                     /locus_tag="IW746_000405"
     CDS             30321..30539
                     /locus_tag="IW746_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077335.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            30552..30962
                     /locus_tag="IW746_000406"
     CDS             30552..30962
                     /locus_tag="IW746_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077336.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            31016..33544
                     /locus_tag="IW746_000407"
     CDS             31016..33544
                     /locus_tag="IW746_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1077337.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            33560..35770
                     /locus_tag="IW746_000408"
     CDS             33560..35770
                     /locus_tag="IW746_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077338.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            35778..36869
                     /locus_tag="IW746_000409"
     CDS             35778..36869
                     /locus_tag="IW746_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1077339.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            36890..37426
                     /locus_tag="IW746_000410"
     CDS             36890..37426
                     /locus_tag="IW746_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077340.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            37648..37878
                     /locus_tag="IW746_000411"
     CDS             37648..37878
                     /locus_tag="IW746_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077341.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            37892..38224
                     /locus_tag="IW746_000412"
     CDS             37892..38224
                     /locus_tag="IW746_000412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077342.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            38268..38606
                     /locus_tag="IW746_000413"
     CDS             38268..38606
                     /locus_tag="IW746_000413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077343.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            38620..38814
                     /locus_tag="IW746_000414"
     CDS             38620..38814
                     /locus_tag="IW746_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077344.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
BASE COUNT        13188 a         6122 c         8291 g        11390 t
ORIGIN      
        1 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
       61 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
      121 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
      181 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
      241 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
      301 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
      361 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
      421 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
      481 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
      541 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
      601 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
      661 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
      721 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
      781 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
      841 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
      901 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
      961 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
     1021 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
     1081 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
     1141 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
     1201 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
     1261 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
     1321 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
     1381 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
     1441 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
     1501 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
     1561 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
     1621 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
     1681 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
     1741 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
     1801 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
     1861 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
     1921 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
     1981 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
     2041 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
     2101 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
     2161 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
     2221 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
     2281 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
     2341 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
     2401 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
     2461 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
     2521 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
     2581 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
     2641 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
     2701 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
     2761 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
     2821 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
     2881 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
     2941 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
     3001 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
     3061 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
     3121 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
     3181 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
     3241 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
     3301 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
     3361 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
     3421 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
     3481 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
     3541 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
     3601 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
     3661 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
     3721 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
     3781 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
     3841 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
     3901 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
     3961 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
     4021 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
     4081 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
     4141 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
     4201 taatggatgt attgttacac aatcttttgt ggttttacct tttcattgtg aatttttact
     4261 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
     4321 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
     4381 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
     4441 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
     4501 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
     4561 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
     4621 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
     4681 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
     4741 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
     4801 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
     4861 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
     4921 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
     4981 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
     5041 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
     5101 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
     5161 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
     5221 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
     5281 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
     5341 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
     5401 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
     5461 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
     5521 aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga aaaacgttat
     5581 ggataagata attttacgtt tgtttggctc tttttttata tacatacaat caattttgtt
     5641 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ccatgtgtat attttgacca
     5701 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
     5761 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
     5821 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
     5881 tgtatggaaa atctttttac aacctaatca ttttggtcga aatttctata cggatgccgg
     5941 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
     6001 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
     6061 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
     6121 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
     6181 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
     6241 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca
     6301 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
     6361 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
     6421 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
     6481 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
     6541 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
     6601 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     6661 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     6721 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     6781 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     6841 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     6901 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     6961 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     7021 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     7081 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     7141 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     7201 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     7261 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     7321 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     7381 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     7441 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     7501 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     7561 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     7621 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     7681 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     7741 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     7801 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     7861 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     7921 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     7981 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     8041 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     8101 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     8161 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     8221 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     8281 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     8341 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     8401 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     8461 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     8521 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     8581 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     8641 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     8701 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     8761 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     8821 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     8881 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     8941 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     9001 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     9061 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     9121 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     9181 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     9241 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     9301 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     9361 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     9421 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     9481 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     9541 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     9601 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     9661 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     9721 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     9781 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     9841 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     9901 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     9961 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
    10021 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
    10081 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
    10141 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
    10201 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
    10261 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
    10321 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
    10381 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
    10441 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
    10501 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
    10561 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
    10621 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
    10681 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
    10741 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
    10801 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
    10861 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
    10921 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
    10981 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
    11041 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
    11101 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
    11161 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
    11221 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
    11281 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
    11341 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
    11401 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
    11461 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
    11521 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
    11581 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
    11641 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
    11701 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
    11761 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
    11821 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
    11881 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
    11941 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
    12001 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
    12061 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
    12121 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
    12181 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
    12241 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
    12301 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
    12361 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
    12421 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
    12481 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
    12541 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
    12601 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
    12661 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
    12721 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
    12781 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
    12841 tgaaacaaat tggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
    12901 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa atcaaccttt
    12961 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
    13021 tcacggcgat actaaaatta acaaaattgg aatatttttt cttgaagatt tttagctgtc
    13081 agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga
    13141 agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta
    13201 gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa
    13261 gacgtagata atactctaag caagattttg aatatagaaa ctctaataaa agatgaggaa
    13321 gataagattg agattctcat cggtcaacta attgcataca gagatatgaa taagttactt
    13381 aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat
    13441 agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat
    13501 gaaaaagaaa tttcgaagga gagtaatcgt gatagtgaga tatttagttt atcaaaagac
    13561 gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt
    13621 gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa
    13681 aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc
    13741 actgttcctt accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga
    13801 gttagcgatt ttaacaagat aaattctttt agtatgggat taaggatgag gaaaattagg
    13861 aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa
    13921 tggtccatag ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa
    13981 tatgcttata taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt
    14041 acgtcgatac ttattaagtt atcgacgttt ttttattata gaaaaattta taaaaggagg
    14101 tttcttgata tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt
    14161 ttctaagtaa ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaaa
    14221 taactgttat gaataccagc tagataaaat gggctaggta tgttatccaa caatttagta
    14281 atcactagaa gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt
    14341 ctttccaaat caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga
    14401 tgggaatcca taccatggaa attcggagat ctgtgaggga atacttacgt ggttaatatg
    14461 tcggttgtgt tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag
    14521 aaaatgaaac caacaagaag atagggttat ttatacagaa ttggggatct tttctagaaa
    14581 agtaatcgga caatgcgaga ttcgtacggt cttttttaaa tttctcttaa aaaaacggca
    14641 tcgaatgggt tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaaatg
    14701 attctattta tctttttaca tctatcgttt tttcaagtaa ccgcaaattg tatcagcatt
    14761 caacaatttt tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg gttgttgtga
    14821 agataaaaaa agaatcaggt tttactgagt ttttcttcaa aatttgtgtt attaggttag
    14881 gacaaagata agaagaagta tgaagattta aaaaaggaat gaggaaaaga tagtaatttc
    14941 cgattattgg agttttttta ttttataaag aagaagcgaa caagttgtga ttcctagatt
    15001 tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta atacacgagg
    15061 acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt gtgattgttt
    15121 cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata agaacaacgt
    15181 ggaatttgtc gagagtgata ttggttggga cgatcatgaa gaagtttatg gaaaatttat
    15241 tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt gttagagatc
    15301 attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt tgaggtgata
    15361 gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg gagattctta
    15421 gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca tgggtaggtg
    15481 tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca gaagatgcct
    15541 atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag tggtttgaat
    15601 gggaaattga aaacggagaa gtaaaagaag aataagcgta cttgttgtat caaaataagt
    15661 cagtagaaca accaaaccag tagctagtaa tgacaataat actggggaat cggatcatta
    15721 tggtgtgatt gatatttcta ttccagaaga tcttaagcca gatcctgcaa caattggcaa
    15781 cagtggcatg atttttgatt cgttagatga agctatgtca tatggttgga aagaaattaa
    15841 ggatgaaggt agtaattggt atggcatgat atttggtgat ggtgaagttg ccaataaagc
    15901 tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt tttttgttct
    15961 gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga gcgccagata
    16021 aaaatagaag agacagttca cgtcgttatc acttttggta acggtgtttt gttttattat
    16081 ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat gaattggttg
    16141 aaagcactcg cattggtggc cttatctcaa ttctgtttct tggtgggaac tgctcaagca
    16201 catgcagaag aaacgacggc tacacaacct acggtggaag ccattgccca agtcgcttct
    16261 gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc cactgttgaa
    16321 caggcccctg tgacagaagc gccaacggtt aattccaaaa cgactctcga tcagacgatt
    16381 ggggaaacac aacacccagg gaatagtcct gttacttcac aaccagttga agaaactgcc
    16441 tcaattacaa cccctgcttc agaaacgtcc actccagaag gagaaaaagc tgccgaagca
    16501 acgccttctg tgaagccaga gtcttctgag aaacaagcgg aagaaaagca acgaatagaa
    16561 gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga ggaaaacgtt
    16621 gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaact aaacaaagaa
    16681 tctacggcta gtattgaaga tcaaaagaaa aaagagcagg acattccaaa aacaggtaca
    16741 cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg tgcaaccaat
    16801 acagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt tacttctagt
    16861 ccgaacaatc caaacttcac tttaaattat atggttcgtt ttgagattaa tggacaagtg
    16921 gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac agctactgct
    16981 ttggaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat ctcttattta
    17041 ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca atttatgatt
    17101 tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta tgatcgtcga
    17161 aaagcagaaa ttcaacgtct gcttaataat tttgatgtaa gagctacttt tcataatcaa
    17221 acacatacga taaaaattgg cgaaacgtta aaagtaaaaa gtggcaatga ctttctacaa
    17281 cgtgtaaaag gcattcaaaa gccaaaaggg atcaatgtat ctattcatgg gaatgaagca
    17341 tggatcacag tcacaaaaga agcaccagaa aaggcaaaaa ttcatttcaa tcagattgaa
    17401 gtattgggta cgccattagc gtatcgtcgt ccaggtgccc aaacaattgg tatattgaat
    17461 ccttttgaac ctggtgactc tgttgtcaat ttgactattt tgaaacaggg aaacattcgt
    17521 gtctttaaag aagatgcaga aaccggtgtg aaagcacaag gcgcagcttc tttagaaaat
    17581 gcggtgtatg gcttgtatca agcagatggg aaaaagatca aagaaattac cttgaaaaat
    17641 atgaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata tgttttaaaa
    17701 gaaatcaaag cgccgaaagg ctatgcactt tctgatgaaa caattgaaat tgttgttgaa
    17761 ccaggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac cattgattta
    17821 gtgaaaattg ctaataaaga tttagtcgat tcaaccaatc cagataacaa accaaaatta
    17881 gctggcattg aaatctcttt gacgtcaaaa acaactggca aagtggtgaa aaccgttgtg
    17941 acagacaatg atggttacgc tacttttggt aaaaatactg ttgtctttga tacctacatt
    18001 ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga agtcacaatt
    18061 tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga acaacgtctt
    18121 aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgccaa tacagaattc
    18181 aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa tgatacagaa
    18241 acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg tacacttact
    18301 tatggaaaag agcgctatcg tttggaagaa atcaaagcac ccgtaaatta cgttttgaat
    18361 caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt gatccatttt
    18421 gcgaaccgtc aagcacggaa aagtgtaaaa ttgttcaagt atgaagaatg gaacaagaag
    18481 aaaacaccac ttgccggtgt gacgtttacg ctatataacg gaaaaggtca agcaattggt
    18541 aaatatacca ccgatcaaaa tggtgagatt ttagtaaaag gtctggttgc caataactat
    18601 tactttaccg aaaatgtacc attagaaagt ttccaaccaa atacaggcaa acaagcattc
    18661 actattacat tcagacaaaa tgcgccagat gatggggaaa cgttattggt ttctgtacgt
    18721 aactatttga ttccaccaaa attagaaaca caagcgtcaa ataaagccga tggtgaaaaa
    18781 gtggttgatc cattagaaac tatcgtcatt aaagatagtg tttcttatac caacctgttt
    18841 gtcggcaaag aatacactat ttcaggtgta ttgatgaaca aggcaacgaa tgcgccaatt
    18901 ttacaagaag gcaaagaaat cacaagtatc ttgaccttta ttgcagacaa agccaatggc
    18961 acaaaagatc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac aatcgttgtc
    19021 tttgaaaaac ttttccgtga tggcattgaa gttgcggtac atgtggatat caatgatgac
    19081 aatcaaagtg ttcgggtaaa caatccaacg gttcatacca cagctgtatt tgaagacggg
    19141 ttgacgatcg cagacccaac ggggctgttc accttaaatg ataccgttga aattaatgag
    19201 atccttccag gaaaagctta cttggtcaaa ggaatcttaa tggataaagc aactggtgaa
    19261 gccttacttg ttcatggtca accggtagaa agtgctgttg gctttattgc caaagcagaa
    19321 aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtttaaatgg tcgtgaaatg
    19381 gtcgtgtttg aaagtctttt tagaaatgac gaagaactag caaaccataa agacatcaac
    19441 gacaaaggtc aaacggtacg agtaacgaac ccaagtgtac gtacaacggc tacacataaa
    19501 cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga cgctgtgaaa
    19561 taccacgatt tgatcgttgg aaaaacatac acagtcaacg gtgttctaat ggacaaagta
    19621 acggaaaaac cagtgctagt agatggaaaa gaagtctcaa gtaccttaac gtttgtggca
    19681 gaaacaactg atggtacagt caatctagac tttatcctag acgcacatgc gttgcgaagc
    19741 aaagaagtcg tagtttttga agatctgttt agagaaggcg tattattggc ttctcacgca
    19801 gatattaccg acaaagacca aaccatcaaa atcgtcagtc caaccattac gacaaaagct
    19861 accaatgcaa atggtggaaa agagatctta gctttaccaa atcaaaaggt tgtcgaatgg
    19921 gttcaagtta atgggttagc aatcggtcaa atttacaaac tgattgttca aggctactta
    19981 acaccagacg gcaccccatt tgaaggaaca catgcggaaa aagtctttat ggctgataaa
    20041 gaaatgatgg aattcttgtt tgaattcatc gttgacggtc gcaaccttgc aggaaaagga
    20101 ttgagttttg cggaatggct ccaagccaaa acagaagatg aaaaagaaac gtttgaagaa
    20161 gtcgcaaaac ataacgtcga tctaacaaac aaagatcaaa cggttcaatt tgtggaggca
    20221 ccaaaaccac cgcaaccagt aaaaccaaac gaaccacaac ctgtaaaaga agtgaaacaa
    20281 gctgcaatgg tacctatgtt gccaaaaaca ggctctgaag acaatgtatt atggatcatg
    20341 attggtatga tctttgtcct tgcggcagga ttgattggtt atgatttgta taaagtcaga
    20401 gactaaagag gacgaacaag cggttaggca actagccgct tgtttagttt ttagaaagaa
    20461 ttttttatca aaggcaaata aattattgtg ttgagtagta aatgatcatg aggatacacc
    20521 aagaaatact gagtgtaata aataagaatt taaggatcca aaagagatag aatataatta
    20581 tgtgataata cagtttcttt ttttctatag aagaaatcac gtttagctac tcctaattgc
    20641 tttcttactt tatttagttc tgctaggtag cagtatgaga attcaaaata cattctttgt
    20701 actttgaaga ggattttttt cgaatgttta tttgtgttaa ttattttttg tatttctttg
    20761 gcaaaataaa taagttcata aggcaaataa aagtctatat ttttattttc aactgtttgg
    20821 attagatgtt gaattgtttg ttttcgttct agaatatttt ttcttgtaat tttttgaaat
    20881 agataatttt caattgaaga tttaaatgga ataaacattt cttttgttag ggtttcagtg
    20941 cttttctttt ttcttgtata gcgatgtttt aaataaaagc tagaaatgga ccaaacaata
    21001 gtgacgattg aaactatact tgagatgata atgtgtaatt catttgaaaa tagttgttga
    21061 ataaaagtgt ttataaaaat aatctccttt tttataaaaa gttagaaagg atgtaaaaga
    21121 gttggaagaa atattctttt gtatgatggt agagtttttg tttgtatcaa ttggtttctt
    21181 aattatgggg gaggcaatta tgatatatct ttttccgttt gacagcaagg atgagtgcat
    21241 ctttagtagt attattattt tctttatttt agcatgtgtg tggattcttg ctagggaaat
    21301 atatcatcag tttgtgttgg aagatggaaa aggaattatt acttggtttt tacagaaaag
    21361 aaaataatgg aggaaaaaag aatatggaaa agaaaaatat aaatatttgt tttttgagtg
    21421 gaaaagaatg tacgtatgag gagtgtactg atatttctgt gcatgaacga gacgggatgt
    21481 taatagtaga tatgtctcct agtgatgagc catttgttgt taaacattac tttccattgt
    21541 gtaatataga gtcgtttaga attgattgtg aattttaaga ggaggaaaaa tgatatggca
    21601 gaaaaagcat tgcgtatgaa gaaagagttt ttaggtcagg tatttttatt gggttcacgg
    21661 gaacctaatc caccaaagga aattaatggt gtgtctgggc gatatgttca attacaagca
    21721 gaatgttcgc gagaagaaca gcctgctttt tttgtttatc tcacagctga agaggataaa
    21781 agattacgat taccagaaaa agaggtacgt atttcatttg aagaagctta ttttgttttt
    21841 cgtaaagaaa gagtgaatag tcgtgaaaca aatgatatgg gacaaacaga gcgtcgaaca
    21901 actggctatc aagatgtcgg tgtcttttta actggtgtaa aaattgagga ggttttgtaa
    21961 gatgggatta aaatttccaa ataattatga tattacagaa gcattagatg aaacttgtat
    22021 aggagccgta cgctttggcg cattagaaga gtccgatcgt gtatttgata atgaaacagg
    22081 cgattatacg ggagaaattc gatcacagcg agcacaatta gtttttggaa atccagatga
    22141 accaaagatt gcacgacgag ttaaatttcc agcagatgta gatttgactg gttttaaacg
    22201 acgtgatcct gtgaaattaa ttgatccaga atatcatgtg gtatatattc cagcacaaga
    22261 tggcaatcgt gaatattcag atttagtgat ttatgcgaaa ggattagaga aagtagaagc
    22321 tccaacaaat aattctaatc cgaaacaatc acaagaaaaa ggtttagatc aaaaacaacc
    22381 aagtgctgat tctaaggaaa aagagaaaaa ataagttttt taaagagtag gaggaagtct
    22441 tcctactctt tctatttaag gtagaaggaa ggggagattt tgaatctgga caaaaccaat
    22501 caaacacttc gagagattat ggtttgttgt caaaatatcc aacaattgat tgagtatttt
    22561 ggagacaatc caacgatttt tatacaaaca aatgagtata tttgtgcggt tattgggcag
    22621 tgtatgtttt tacgtgatct tgttcgtttt tcattaccgt atcagtggaa acaagaccat
    22681 caggaagtgg tttggattcg tgtagtacga atatgtaata tgtgtgcaca cccatataaa
    22741 aatcgattaa atctttttcg attatggttt tttattaaat gtgatattcc agtattagtt
    22801 gctttttgtc aaaaattttt agcacaggag aagtaaaaac agaaaggagg aatgtgacaa
    22861 ttggaacctc atttttttaa gtatcgtggt cgacgaattc gttatttcaa tcgttattta
    22921 tggttttgtt attatggttt ctttctgtta ccatttttga ttctattaag ttggtttagt
    22981 tggaaagtat tttatcctcg tttaaatacg tattcgaatt ggcaaatgta tcttattcct
    23041 ggaatcagta ttgtgatgta tttgttgctg gttagtggat taattttagg tttgatgcgt
    23101 tggtcgcgac taaaggaagg atattttgct tctgtttatt ggcgtcagtt acttgctcga
    23161 atgttaattg acaatcggtt tgtttataca aagaagcaag ttggtgagaa acaaacgaag
    23221 gaaaaaatga gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct
    23281 tttcctttag atggtggccg tttccacgaa cgatttttaa aaatgggtag cttattatcc
    23341 gaagtatttt tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta
    23401 tttctttccg attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt
    23461 catttaatga aaacattgga ttgggtatat gatcaatcgc caaatctatt aatttctggt
    23521 gcgattggtg gcggaaaaac ctatttattg tattcattga tcaaagcttg tttagatgta
    23581 gggacagtag atatttgcga tgggaaagcg gcagatttag cggcattagg tgatattgat
    23641 gtattcaaag gacatgtgtt ttacaaaacg aatgaagaaa tgattatttg cttacgaaat
    23701 gcgttacaag aaatgaatca gcgatatacc tatatgaaaa cgatgaaaga gcctaagtat
    23761 gaaccaggga aaaattatgc ttattatggg ataccgccgc attttatctt ttttgatgaa
    23821 tgggcagctt tctatggttc gttggatatg gaaacgagaa acaaagttga taaattaatt
    23881 cagccattag tattaaaagg tcgtcaagca ggcatgtatg ttattttagc gatgcagcga
    23941 ccagatgcgg aatattttcc aagtggtgtt cgagatagtt taacgttccg tatttctgtt
    24001 ggccgtttat ctcccattgg ctatttaatg acttttggag aggattttaa gaataagccg
    24061 ttttataaca aagatcaacc tggtcgtggg tatgcttttc ctggttttga tgtgcctgta
    24121 gaattctttg cgccatttgt ttacaaagac tatcacttta ttgaggaatt taaaaaattt
    24181 gacacaatga ttgtgcaaga tttttctgta ctgaaagaag aggcgtttac tagttacgaa
    24241 gaacaaattg atcgtcaagc gcaatcgtta aaaggaaata cgaaaacaaa tggggtagat
    24301 gaggaaagtt atacactttt ataatttaga aggaagtggt gaagtatggg tgaagttttg
    24361 tatcgtgttt cttcagctgc gggtgaaatt tcacctgatt ttgcggttcg aagattatac
    24421 gagtggatta acaaagtaga atactacaca aaaggttctt atgttttccg acgaattgag
    24481 cgcgagacat tgtttgttac gagaaatcag attgttttga caaaagagga tatattgcgt
    24541 tttcgtcagg tatatcggtt atgcaaagag aaaaacatac agttacgttt agcgatttta
    24601 cagtgttttg ctcctgaaca gtataaagaa ttacaagaaa aagaagattc attggtttag
    24661 atatcggggt ggctttcata gggactatgg gagaacacat tgtccgagta tgtgctgccg
    24721 cggcttgtgt cggcgaagcc accgagacca gtcgtggcag cacatgcttg gcatgtgtgc
    24781 ccacaaagag gtcgtgcgct tgcgcacggc ctctttgtgt aaagcccccc gatatataat
    24841 atggggggca gaaataacat aacacaaata aaaaagtgta tttaaatcag ttatatcaag
    24901 gctttagcta aggttagaag gagtactcct ttttagaaaa attagggggt accacctgtt
    24961 atatcgggat ttctagcaga ttaagaaaat cgaaatttgt tattttataa aggaggtaga
    25021 atatgacacc tattgaattg cgaaattatc gaaaaaaatt gagtgctatt cacgcttcag
    25081 gtggagggaa aaaattactc tctcaaaaac gttttgcaca gttattggga tgctcggtag
    25141 attatataaa gaagatggaa caaggaaaaa agaaaatccc agaggatttc gaaaaacgaa
    25201 tcaaggaacg ctttcgattg tatatgaaaa gcggaataga tcttcgagca atgatcgact
    25261 atttgcgtct ctcctttttt gggacagact acaaaatagt gattcaacgt gtgttacgaa
    25321 tggatctaac agaatttgaa acacgtccga cgggcttgta tctttttgat tgtctttctg
    25381 ttcgtggaaa tatctgggta ttctggcatt caaaacaaac tgtaacacaa aatgtacttg
    25441 tacagttttc gggtcaaggt tgccgagaat atgaggaagt catgcgtgaa caacgaacag
    25501 attggcgaac atggctatat tccatttgga aaaatcaagg aaagttggaa gctggttata
    25561 aacgagtaca atgtagtcga ttcgatattg cgttagatga actttgggga ttggaaacag
    25621 aagaacattt tgatctcttt gaattactag aaaaacaaaa agcaggttta ttagaaatgg
    25681 actttaagaa atttcagaat attggcggtt gttttttaga tgatggttat ttccgaagcg
    25741 aagggttatc cttgtatttt ggatcgcgaa agagtccatt gtttttcaat ttctacgaaa
    25801 aacgttttga aattgcgaat cgtgagaaaa tttcagaaat agaagcatta acgaaatatg
    25861 gcatttataa tcgatatgag ttacgcctag ctaatgaaaa agcaacacaa gcagtagaag
    25921 cgtttatttt ggatcattcc cctaaacgtt taggagagat tggcgttggt ttaattaatg
    25981 caaaacttat tgtttgggat cgactggaaa gtgatgcacg aattgttgat gaacgatggg
    26041 caaaattgtt tcgttcgatg gaagaattgg attttaccgt aaagccagaa aaattctcat
    26101 tagatcgttc agaacgctgg tttttgaaac aagtagctcc gacattaaag ttattacaag
    26161 aagcagatcg tattttagga agagactttg tacaagaagg aattgagaat gctgaactaa
    26221 atgacgagca acaaagctat ctagaatgga tgggaacgtc aggagggaaa attgcatgaa
    26281 tgcatatacg acaacagaat ggctgcgtct tttggattta aaggctgcaa ttgaagcatt
    26341 aaatgaaaag atggttgatt tgtcttattt tcgttttcga gttccttata ttgaacaagc
    26401 ggtaaaggct ggacgttatc aggaaaaaga aaactggcaa gaaattgcac gcttattaga
    26461 agttcgtaag gggtacgaac aagaactcga agagttggaa tttagccgaa gaaaaggaac
    26521 gctaaaattc attcgctttt atcgattttc attgccggta cctgcgattt tagcggtaaa
    26581 aaaaggctgc gataaaatga agatatacga aaactgtgtg gcagcactta gcaacgaaaa
    26641 acctcttgtg gaagaaatct cgttggcaac tgttacttgg gaaatgaatc gccagccaac
    26701 agaagaacag acgtatcttt ctttagaaga gattgagctg gaattagagg aaatcggccg
    26761 ctatgccact tgttctacct attgtggatc ggtgattagt attgctggtg tgattgtcta
    26821 aatgaaaaaa attatgattt atgttggtgc gtattggtcg cgtgatccaa cagtattaga
    26881 aaatccagaa gcagtacact attgcttgcg ccaattattc tatctgtata aagaacgatt
    26941 ggaatcattg attcgtcagt tgccatatac tgacagacgt ttggatgaac ttcttctaag
    27001 gtatccagca atgtataaac gacgaaaaaa tagattgtta ccaaaagagt atcccattga
    27061 aaaacgtgaa cttgaagggc gatttgtggc ttatttctat gatgatgttc gtatgcgctt
    27121 agtagaacaa cgaatggaaa tagaaaatga tcgctactat tttatcaatt attgtaagaa
    27181 gcgtaagtat caagtaactg atgattttta tgattgtatt ttacaagatg gggaagtgat
    27241 tctatctaaa attgctccaa gttttcgtga acttgtgccg attaattttt tgaaaaaatg
    27301 tcatttgcgg attcttccgt aaaaaagagc tataaaagga aagaaaagta gaagccaaat
    27361 cggtttctgc ttttttattt tttaaggaag gagaagatgg tatgtcactg gaatttttag
    27421 gagagccaat tgaaaaggaa gagttcgttt cgcattatat ggatttgttt attcgggtga
    27481 taaaaaagct ttgtacgatt gatgaatttt atccacgcga acaagactat ctagccgctg
    27541 aacgaataaa aatcagttta ttatgggaaa aattcacaga tgaaaataac cgaccgccag
    27601 attatcgcta tttatcagat tgtttaacga ataatgtgct ttctaaagaa tacctatttc
    27661 acaaacgtga gaaaaatcaa atgtcggctg aacatttttg tgattgttat attgcattat
    27721 tgcgtagtca acagttatta acggtagaac gatatggtgc agagtttggt tatgtcatgc
    27781 aagctgagcg tcatagagct acaagatgct atgagaaaga agatcaagtg attgatggct
    27841 atgaagaatt atgtattcga aacaatcgct atttgcaaca acgtttatac gaaaaattaa
    27901 tgatggattt ttcggatctc taccgagggt atgaatgttc ggtgattcaa gaaaggtaga
    27961 agaaaagggg gaatttttat gtggcgatta ttatttttgg tggtatgtgg tatggcattg
    28021 ttctatgtgt atcgtgtcta tctcggtcca cgaacggaac gatggacaag ttggtataga
    28081 gatcctttaa aacaacgaaa aggattgaag cggtatcgtt atgaattact tgttgaattt
    28141 tcggatgggc gacaagcaca gtattatttt tttgcaaact atccaaaata caacaccagt
    28201 gaactattga ttcaattatt caaaaataat ccatatgttg ggttagaaga aaacggacgg
    28261 gagttctttt atcgttcaga taatattagt gcggcgtcta tgaacaaaac acgaattgca
    28321 taaaaaattg gaggaataat catgaaaaaa acaacattag cagtcgtaac aacactagga
    28381 atttcattat tttcgtttca attagggagt tcattggttt atgcagatga aactgtagaa
    28441 acacctaagt cagaaattgt agaaacattg ccaggggaag gattatcaga tgttgagaca
    28501 gagctaaatg gaggaacaac ggaaaaacca gcagaacctg aacatttgca gccagagaaa
    28561 cctgaaaaag agccagtggc accagaacca gagattcctg aatcaacttt gccgactgat
    28621 ccaattgaag aagaaggtgt gcacagttca acaacaacgg attcaagtat agtcacaact
    28681 ccagaacaat caggaacaac cagttcttct gaaacgaata ccgattcgtc aagtacggaa
    28741 cagccaacga tgtcgtttac cagtgaagaa ccaaaagaag aaccgaaaca acctgctgaa
    28801 aaatcagaag caattccaaa agaaaataaa cctattgttc cagcaaaaga agtcacagta
    28861 tcggtaaccc caaacggtga aattacgacg aataattctc aaggaatgag tgtaccgatt
    28921 gtcactagta atgtagagga attgacccat attccaacac caacaactcc actaaaagca
    28981 gaaggtggac aaacaattgt tggtgtaaaa gatggaattc cattaatcca agatagtgaa
    29041 gggaatttgg taaaggattt gtcgattcct gtaaagaaac ttccaagtgg caatattgaa
    29101 gtcaaaacag cagatggaaa aacaaaagtc ttaccaaaga ctggtgaaga aatacatgtt
    29161 gtactttctg ttctaggagg agtgttgaca agtattgctg gatttgttgg atataaaaga
    29221 aagagaaaag gaatagaaaa tgagggtaat tgaatggact atttttcaga aaaacaagct
    29281 tatcgttgtc aatcgtacgt actagtaaaa acatactaga aggagtagct tatgaaaaaa
    29341 cgacaactac ggcggaaaga aaaagtttat acagagaaag taccgaatca aaggaaaatc
    29401 cattcaaaaa aagcagtgaa atggatttgg tgtttggttt tatttctttg tttttcaggg
    29461 ggcttggcat tttttcaatc catgaaaacg aaggttcaag tgtcgttttt gaaagatgaa
    29521 atccaacaat tacaaaagaa agaagtacaa agtcagtcag aattaacgta cacacctgaa
    29581 attgaatcct ttatgaatcg ttttgttgct ttgtatatga atacattcga taatcatgaa
    29641 caagaacaac aacgtcaaga aatattaaaa aaagagtttt atgcacaaaa ctttattcaa
    29701 gaaccaggaa gtgtttcagg tattcgaaaa ttaactgctg cttcgtttat tggattaaaa
    29761 gaaattgaaa ccattccaac agctagttat aaagtaactt atgatattcg ctcgatggat
    29821 ccagagaata agaaagaaac aagtgtgaca atcacacaag tactcaatct tcccttttct
    29881 atttcaaaca agggctgtcg tattattgct tatccttatt ttacggcact accaaagaat
    29941 acagaaaaac aacgaatgat tacttatgat gacacattat acgaaacggt tgatgcgtcg
    30001 gaagcgaaag atgtggaagg atttgtggaa gatttcttag ataagtatgc gaaaaacaat
    30061 gcaaaagata tgtcatacat gatgaaagaa cctgaaggac tcaatcagac agctaaagtt
    30121 gatcaagtag agatgaaagt attgcgttat aaagaaacga aaaaaacttt tattgtaaaa
    30181 gcaacagtcg tctttcgaga tggtgatacg tcacttttac aaaaagaaca aatgacgctt
    30241 gttattacga aaaaaacaaa tcagtattat gttgaaaaac tcacacataa ttggttggat
    30301 aggaaaggag acgaacggaa atgacactga agggaattgt agattttata ttaacgcaag
    30361 gtggctattt tttaataatg gtggttgttt atctgggatt gcgaaatttt atgaaatcaa
    30421 aagtaggaaa tgcattaata tctatcgcag caggtgccgc agtctatttc ttcttaaatg
    30481 atccaacgcg tgtatttgat gcgtttggta aaatcattga aaaattattt atggattagg
    30541 aggatagtga agtggattgg gaaccttacg attattcgat tgtttatgcg gagccttatc
    30601 gcgtacaaca gattacaagc aattggcgtc ttccatttcc agttgcatta gtcaatgcag
    30661 caactttttt attggtcgta atctttgaat atttattttt acgttcattt attggttggg
    30721 ttatttcttt ttttccacca gcaggggttg ttttttttat agggctaccc tatttatgtg
    30781 tgttagcact ggcaaaacta aaacctgatg gaaaaaaagt gcatttttat ttatgggatt
    30841 ggttttgtta tgtagtgcgt atgatgaatg gtgattgtgt gtattataac tgggacaagg
    30901 tatcaaataa tgcattggaa gaattacttt ttgcgaataa gtatgtttat ttagaggatt
    30961 agaaaaaggg acaatcaact ggttgttcct ttttttaatt gggaggtgag atctattggt
    31021 acttgtaaaa gaaccagtga aagcattaaa gaaaaattgt ttgttaaatg gcgaggattg
    31081 tatctgggcc tactatcatg tttttccacg aaccatttca aaaaacacac caaaaaagac
    31141 acaggaaata aaagaaatga tggctcgttt actgttactt ttagcagatt atgaagatgt
    31201 ggagttggtg atgtatccac gaacaatgaa tttacggacg aaatttgaca cattaggaaa
    31261 gggattttct cccgatagtc aagcaattgg tgcgtattat gcaaaagaaa cagtgacatt
    31321 gttggaacga gaaggtcaag tctatcgtcc ttcctttatt ttaggaatca aagtcaaaga
    31381 aaaagcaacc gtggagagtt tttatgatgt actaaatcta atctggttag atgtagcagg
    31441 aagtttagca cgcttatttg gattagaagt agatgattta gaaactttgt tttctgaaag
    31501 tgatccttat gtaaaagaag tgacacaatt actttatagc ttttctattc gtcccttagt
    31561 aaaaaaagag ctgttgtatc tcaatcgtct gcaatatatt cgaggaatgg ctcataaagt
    31621 agaagaacaa aataattggg aagtactgaa agacaatcaa ggaacgtatg tacgtatgcc
    31681 gaaggaacat aaaggccttc ttcaattaga agatgagttg gggactagtt atttagcgtt
    31741 gttacctatt ggagaaacac ctgaaaatat gagaaactgt gatctatttc aatttgcaca
    31801 ggatctttct ttcccagcag aattacgaat gaaaattcac tatccacgct tgaaaggaca
    31861 gggaggtatt ggtttaaaac taaattggct atcggaacgg ttcaaaacag aacataagga
    31921 attggttcaa aacgatgaag aacctagcga acgcttatta actgttcggg gattagtaaa
    31981 acatttaaag caacgagtag ataatggcca acctgtcgtc gattggatcg catgttttat
    32041 tgtatacggt caaacggttg aagaagttcg tcaaaatagt gcaacgatta ttcaacagct
    32101 tcatacgact atccatatcc accgtgcaaa aaatgatcaa cgagaattgt tctataaatg
    32161 tttattaggg caaccattgg gtcgacagtt gcattggaag caccgaacaa ctgtagaagc
    32221 aattggcgaa tggttgtttt cagcagtatc agaattaggt atggaatccg gctggtatat
    32281 tggtcggcaa gatacattag gggatggtgg ttctcgagga gaaaaaacac cattagaaga
    32341 attaatttat gcgtcaaatc gttttgtctt tttgaatctt ctagctattg cggaaggaat
    32401 caaaggtgcg ttgtttgatg cgcctcatgt agcagtaact ggaaaaaccg gtaaagggaa
    32461 gaccttttta gttggattgc tctttttgta tagttcgttt atgaatgtac aaagtttatt
    32521 tgtggatccc aaaggagaaa agcgcaaatg gtttacgaaa ctcattcaag acccgtatta
    32581 tcagaaacat taccctttgt ttgtggaaca tttgcaaaaa atgagttatg tcacgttaga
    32641 tgctacaaat ccccgtaatt atggcgtatt agatccaatt gtttgtttat cccgagtaga
    32701 tgcgaaacaa gtagcccagg atatgattga tgagttaagt ccgttaggta ccaatcacct
    32761 tttaaaaggg gcggtattaa agggaatcca agaagtttta gttcaaaaag aagccggaca
    32821 acaagttggc ttgatgcatg tggtcgagta tttagaacaa atggaacaaa aagaggttcg
    32881 agaatatggt gagtttttac gcttgacggt agaagattcg attttacgtt taggattttc
    32941 ttatggagaa aacccgggat tagactttaa tgccaaaaca acgattttag agatccaaga
    33001 tttgaaatta ccaaatgaca atgtccgacc agaactatat acagatgcgg atcgaaagtc
    33061 tttgtgtttg atgattagtt taggtcgttt ttgtgaaatg tttggaaaac gagatagtag
    33121 taagaaaaca gcgatttatt ttacagaagc atgggtattt aataattcga acgcgggacg
    33181 tagtattatt gcagcaatgg cgcgtgtggg gcgttcacag atgaatcaat tagtgctaga
    33241 tacacaattt attggcgact taggttcaga taaagaaaaa gggaattttg gtgttgtctt
    33301 cgcttttgat gaagacagtg agcgagaaaa gattttgact catttacgtt tggaagtaaa
    33361 tgaagcgaac ttatctgaaa tgagccatat gattaaagga cagtgttgga tgttagatcc
    33421 ctatggacgt gtaggaaaac tcaatgtaca cagtttgttt gaagaaatga ccgctgcatt
    33481 acaaacaacg gaaaaaacaa gtcactctca aatagaagaa caggatacgt taatcatgca
    33541 atagaaacga ggtgaaacaa tggcttatat aaagcgtaag gaaaagaaat ggtgcgtttg
    33601 gcttttttta acggttggat tcggactgtt ttttgttggt ggattggtat atgcggtaga
    33661 aaaacaggtg gatcaatcga tctatgagat cgggaaattt catattgata ccttttcttc
    33721 ttatttatct gatagtgaat tttcattagg caaaatgtta tttggagcaa atggcacaga
    33781 ttttctaaaa atgatgacga atatgttctt tagtttaagt aaattgattt ggcaaggcct
    33841 tgattttgtc attgagaagc tctatgaagg agctgcaatg aacgagttaa ttcaccagtt
    33901 tttcactttt tcggatacta tttattcgaa aatatacgat cctattggtg cggggattgt
    33961 cgtcttgtat gtgttatatc tatttactct tcgtatattt agagggaaac attacgcaaa
    34021 aatggtgttt attcgatttc tctttgttgt cattttttca atggtttggt ttggctatgg
    34081 tagtaacgta cctagtaaag gagaagcttt tactaaaggg ttaaatgatt tgtccgtgga
    34141 ggtagaaggc tttatttttt ctgctacaaa tagtctagat gaattggcga caaaagatgt
    34201 caatggtgtt ttagtgccaa cgacacaaaa ggaagccatt caaaaagtgc gccaacttta
    34261 ttataaagcg gctgttgttg atccgtatct attgttaaat tatgggacaa caaatttaga
    34321 agaattgtat caagcagaga ttgaaccaca agaatttcta gccaaggaag cgtcaagtga
    34381 agaattatct gatattgaga caaaagtgac cactgctgca ggagaagata gagggaaaaa
    34441 gaaggcttat cgtgtctata ttcaaccaac taaagctgtt tataaagcag tcgttggttc
    34501 tatggtgcca ttgcttaatc taagtattgg attaccactt gtagtgattg gcatgattcg
    34561 ttttatcttt caaatgttgg tgttgtgttt attacttgtg attccttttt cattactcgc
    34621 ttcttttgtt ccagcatttg attatatgat catgaatacc ttaaagtcct ttttgagctt
    34681 gatgtttcaa aaatcttttt acagtgtgat tattttggtg gcatttttga tctataacat
    34741 agtagacgat ttaattctaa tgaatactgt ttttggtttt ttaggaaata tgtttgtcaa
    34801 agcactccta tcaattttac tcattgtaaa acggaaagat ttattaaaga aactagggtt
    34861 aggagctgca gatcaaacca taaataacac gaaaaacata gcaagtcatt ctttgtctca
    34921 aatgaaacaa ctgcctggtc aactatcaac tgctgggcaa aatttggcaa tcaaaggtgc
    34981 aggattagca gggaatgtgt accgagcaac gaatccactc gcttctgcag cgatacatgg
    35041 atttcaaaag tttcaacaaa aacgtacacc acaagaatcg gtaaaagggg ggcaagtgat
    35101 taatcccttt gaactaggtg ggcgttcaaa tcaagacttg ccttatagtg gtcgtcatgt
    35161 tcgttatcat ggggtagagc ctgaaatgtc ttctaccaaa tcaaaaacag agtggaattc
    35221 tggtctttca aatggtttga ttccaaattc gtccatgaat tcagtaagac cgaaacaaca
    35281 ggtacaatcg ttggcacaat cgagtaagca acaagggaat aatcaaagaa aaccattgtt
    35341 aaataaaggt atcgcttttg ttggtcaatc gattccgttg cctgctggaa aagtacccgt
    35401 gccacaatta acagctgctg gttctctttc acaacagatt caccaagtga aaaatcaggc
    35461 gaaaaatggg agttatccag caatgcagca gttattgaat caatactctg ttcctaagtc
    35521 gtcggtagcc actctatcag gaaatcgttc tgtgcaacga cagatacaac gttcgtctga
    35581 aagtaaaccg tcgatttccg taccaaaatc cttacaacct gcattttctt cttttaataa
    35641 tccagtagag caatcgaaaa aaatagaaaa gaaaaatgaa acattgccga ttttggacgc
    35701 aaaaccagga accgatttta ctttaccaaa aggacgaaca gttcaaaaac cattatcgaa
    35761 gaaaggatga ggaagaaatg aagaaagggt gtctaggttg tctgcttttc tttattcttc
    35821 ccttttttct attgccattc tttttttcag gagaactttc cgaggatgga ggagaggcta
    35881 caggtctagc tgtcgctttt aattgggaat ataccgaagg attaccgatt ttttcagaga
    35941 ttaaaggacg tggtccgatc acagatgaac atgcgcaagc agcagtcggt gcagcggtaa
    36001 agtatcagtt attaccaagt gtgattcttt ctcaattagg ttatgaatcg acttatggac
    36061 aatcacaatc tggaagaaac gatacaaact tctttgggat tacctggttt cctggttgtc
    36121 cctatccaca aggttctgca cgggggattg gtggttctga aggtggcttc tatatgaagt
    36181 ttccgaacct gaaagattgt tatgactatt acggcttttt ggtggcgagt caaagcaact
    36241 ttaatcaatg tgtgggcaat aaagatcctg ggagttgttt attgattcta ggtcgtggag
    36301 gctatgcggc ggcaggtatt accgaaggca gtccgtatta ccaaggagcg atgagtatca
    36361 taaagtctta taacttaaca gaatatgata cttttgcgat caaacattgg aatagcctac
    36421 caacaaaaaa atcgtcactt gttgcaggga aaagtaaagg taatgcgaaa gttttagatc
    36481 ggatcattgg acgatcggta aataatggag agtgttatgg tggcacggcg tggtatgtat
    36541 cccaactagg tggcccacag ttaatgggtt ctggacacat gtttgcgatg ttaatagggg
    36601 aagattatga ttggacagcc tatggttggc aagtgatcat gaacccaaaa ccaagtgatt
    36661 tacaagcagg agatgtggtg aattggcaag caggtggtgc attgtctcct ggaatctatg
    36721 gtcacaccgg aattataaca gatgtttcag atggcggaca acatttctct agtgtggaac
    36781 agaatgccga gcaaggacgg attatggctc ggtatcaacg cacgtacaat caaacaaaaa
    36841 ttcgtagtct ggttcgtaag gtgaaataag aaaggcagtg tctagaagcg tgaaaaaagg
    36901 aataatttta gtatttagtt ggttatgttt gataggtctt actggttgtc attggttttc
    36961 ttctgaaaaa caaaaaacac aattgacaac gacagaagaa acaatcgaag cagtaaaaga
    37021 aacaagtgga actcttcaaa gtactgaaag taacagtcaa attggttcat tagaagacga
    37081 gacagcgcaa caaatagatg aaatgacaaa aaatttctta aaagattttg ctagaaaatg
    37141 gagtaatttc acagatgttt ataaacgcaa tcaagctgtg agggactata tgacggatcg
    37201 ttgtattaaa gacaatagta ttgatgtaga cccgcatgta caaatgaaaa caactgggaa
    37261 acttcaccag gtgacacaag atatagagaa tccggatcaa tatttgttat ttggagaaga
    37321 aacaacgaat gaggtcactc aatttgtatt acttcaattg aaagtagatc aagaagctca
    37381 aaagattgat gcgatcaaga tttattatgt acgaagtgtg tattaaaata aaaaatgaac
    37441 actcatattt attatgcacc gaaaaaatag aaaaaaacag actgtttttt taagaagaag
    37501 tatgtcattc tttacttgaa aaaagttgtt ataggacaga aaggataaaa caaaaacgga
    37561 aaaaatatgt cttatgaata actacaagaa ctattctatt ttttaggaga tggatacgag
    37621 atgaaaaaat taattggcta tagtgtgatg tgtattggtt ttgttggtgt gggattgttt
    37681 gtatcgactg aatctgcttc tgcggctgaa tggtataatc ctatctggta taatccgaac
    37741 attgaatgtc aaaaagtcta tccgtatgga ccgacaaatc tgaaaaatgg aaaatgtaga
    37801 gtaaattgga atggcgttgt agatagtgta attactggtt caatggactc tgctctacat
    37861 ggctttacgg gtcactagaa ggaggaagag aatgtctgaa gagaaaaaag aaatcgttga
    37921 atcattagta gctttaaaag attctctttc taagattgaa tatgttgata ggaaagaaat
    37981 tcaaaaacta attgatgata ctattattga aatccaagat gcgcgttgtg aaggtataaa
    38041 aatttctgta gctttatcta aagtaataga aaaaatgaac cgttcattag cgtttaatgg
    38101 attgaagcta gatagacaaa ctagtctggt atgggatcat ttaaaagact tatatgacaa
    38161 gtctaaaaga agtgaacgga ctgctgtatc aatcttaaaa ggcctttggg ggatgaattc
    38221 ataatcaaga tattttaaaa ataacttatt tttaagtagg aaaaattatg aaaagaatag
    38281 ttaaaatatt tggtatttta ggtgttttct taataggttt aagtactttt gttagcgctg
    38341 gaataagtgt cgatgcttct gaaacaaata attttttagt aaaacaacaa gaaactgaaa
    38401 taaatatcat tcaaaataat ttagccacac ataaagattc taaaggaaat cgattagtat
    38461 atattattga tgagagtgat ttaaaatcaa gtttagatag aattaattct gaaattacag
    38521 tttctgaaat ccaacagtat ctaaatgaat ttaatcgttc tattcaaaaa aaatggccat
    38581 ggatggctaa ccgatcagct tcatgaagtt gagaattata tgaaagatca aaaaacaaat
    38641 aataaaactg gtgaacgaag tgcctgcagt tgggctttca ctggggctgg acttctacat
    38701 tctgggattt atacagctgc tgctctaggt ttaagcctta cgggagtagg tgcagccgtt
    38761 attccatttg ctgtaggaac agcctatgga atcgcaggag ctgcgatctg ttaatatgaa
    38821 aaaaaacaat atcaaatagt tcatttttta attactttat tatttagccc acttttttat
    38881 agaagtttag ataaatcagt agtctttacg atgctagtta ttttactttt aaatagtacg
    38941 agtttctatt ttttgaaaaa ctatcatggt agaaaaaaac tttttccttt a
//
LOCUS       DACEND010000009        38594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEND010000009 DACEND010000000
VERSION     DACEND010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.010"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(361..987)
                     /locus_tag="IW746_000415"
     CDS             complement(361..987)
                     /locus_tag="IW746_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077345.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1282..1617)
                     /locus_tag="IW746_000416"
     CDS             complement(1282..1617)
                     /locus_tag="IW746_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1077346.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1604..1888)
                     /locus_tag="IW746_000417"
     CDS             complement(1604..1888)
                     /locus_tag="IW746_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1077347.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1944..3467)
                     /locus_tag="IW746_000418"
     CDS             complement(1944..3467)
                     /locus_tag="IW746_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1077348.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3460..4635)
                     /locus_tag="IW746_000419"
     CDS             complement(3460..4635)
                     /locus_tag="IW746_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1077349.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4639..4824)
                     /locus_tag="IW746_000420"
     CDS             complement(4639..4824)
                     /locus_tag="IW746_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077350.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4790..6484)
                     /locus_tag="IW746_000421"
     CDS             complement(4790..6484)
                     /locus_tag="IW746_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1077351.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6481..6954)
                     /locus_tag="IW746_000422"
     CDS             complement(6481..6954)
                     /locus_tag="IW746_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ1077352.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7023..7178)
                     /locus_tag="IW746_000423"
     CDS             complement(7023..7178)
                     /locus_tag="IW746_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ1077353.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7312..7692)
                     /locus_tag="IW746_000424"
     CDS             complement(7312..7692)
                     /locus_tag="IW746_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1077354.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7696..7911)
                     /locus_tag="IW746_000425"
     CDS             complement(7696..7911)
                     /locus_tag="IW746_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077355.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7914..8321)
                     /locus_tag="IW746_000426"
     CDS             complement(7914..8321)
                     /locus_tag="IW746_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ1077356.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8587..10062)
                     /locus_tag="IW746_000427"
     CDS             complement(8587..10062)
                     /locus_tag="IW746_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ1077357.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10052..10900)
                     /locus_tag="IW746_000428"
     CDS             complement(10052..10900)
                     /locus_tag="IW746_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ1077358.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10937..11299)
                     /locus_tag="IW746_000429"
     CDS             complement(10937..11299)
                     /locus_tag="IW746_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077359.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11599..12223)
                     /locus_tag="IW746_000430"
                     /pseudo
     CDS             complement(11599..12223)
                     /locus_tag="IW746_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12220..12531)
                     /locus_tag="IW746_000431"
     CDS             complement(12220..12531)
                     /locus_tag="IW746_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077360.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12575..12868)
                     /locus_tag="IW746_000432"
     CDS             complement(12575..12868)
                     /locus_tag="IW746_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077361.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13062..13709
                     /locus_tag="IW746_000433"
     CDS             13062..13709
                     /locus_tag="IW746_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077362.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13770..14915
                     /locus_tag="IW746_000434"
     CDS             13770..14915
                     /locus_tag="IW746_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1077363.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14996..15301)
                     /locus_tag="IW746_000435"
     CDS             complement(14996..15301)
                     /locus_tag="IW746_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1077364.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="IW746_000436"
     CDS             complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="IW746_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1077365.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15754..16020)
                     /locus_tag="IW746_000437"
     CDS             complement(15754..16020)
                     /locus_tag="IW746_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1077366.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16275..17000)
                     /locus_tag="IW746_000438"
     CDS             complement(16275..17000)
                     /locus_tag="IW746_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1077367.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17361..17431)
                     /locus_tag="IW746_000439"
     tRNA            complement(17361..17431)
                     /locus_tag="IW746_000439"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17397..17399),aa:Gly,seq:ccc)
     gene            17548..18300
                     /locus_tag="IW746_000440"
     CDS             17548..18300
                     /locus_tag="IW746_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1077368.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18316..18795
                     /locus_tag="IW746_000441"
     CDS             18316..18795
                     /locus_tag="IW746_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1077369.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19046..20626)
                     /locus_tag="IW746_000442"
     CDS             complement(19046..20626)
                     /locus_tag="IW746_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1077370.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20619..21362)
                     /locus_tag="IW746_000443"
     CDS             complement(20619..21362)
                     /locus_tag="IW746_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077371.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21538..22314)
                     /locus_tag="IW746_000444"
     CDS             complement(21538..22314)
                     /locus_tag="IW746_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1077372.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22562..23398)
                     /locus_tag="IW746_000445"
     CDS             complement(22562..23398)
                     /locus_tag="IW746_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1077373.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23409..24338)
                     /locus_tag="IW746_000446"
     CDS             complement(23409..24338)
                     /locus_tag="IW746_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1077374.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24530..24883
                     /locus_tag="IW746_000447"
     CDS             24530..24883
                     /locus_tag="IW746_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1077375.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24957..25613)
                     /locus_tag="IW746_000448"
     CDS             complement(24957..25613)
                     /locus_tag="IW746_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1077376.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25839..27773
                     /locus_tag="IW746_000449"
     CDS             25839..27773
                     /locus_tag="IW746_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1077377.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27859..29082)
                     /locus_tag="IW746_000450"
     CDS             complement(27859..29082)
                     /locus_tag="IW746_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1077378.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29076..30974)
                     /locus_tag="IW746_000451"
     CDS             complement(29076..30974)
                     /locus_tag="IW746_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1077379.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30968..32086)
                     /locus_tag="IW746_000452"
     CDS             complement(30968..32086)
                     /locus_tag="IW746_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1077380.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32169..33002)
                     /locus_tag="IW746_000453"
     CDS             complement(32169..33002)
                     /locus_tag="IW746_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077381.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32995..33912)
                     /locus_tag="IW746_000454"
     CDS             complement(32995..33912)
                     /locus_tag="IW746_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077382.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33935..34114)
                     /locus_tag="IW746_000455"
     CDS             complement(33935..34114)
                     /locus_tag="IW746_000455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077383.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(34139..35263)
                     /locus_tag="IW746_000456"
     CDS             complement(34139..35263)
                     /locus_tag="IW746_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1077384.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35446..36354
                     /locus_tag="IW746_000457"
     CDS             35446..36354
                     /locus_tag="IW746_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077385.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36495..38108
                     /locus_tag="IW746_000458"
     CDS             36495..38108
                     /locus_tag="IW746_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1077386.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11679 a         7700 c         6334 g        12881 t
ORIGIN      
        1 ctgtgaagtc aacgttttga cctgattatt agagaaacaa atggcaaaat agttcttcat
       61 atttcgccgt ttttcgagcg aatgccgagg ggcagctgat tgaacatcgt ttatttggaa
      121 aaagggattg tgacaagacg tttgtcacaa tccctttttt tatatcttat taattcattc
      181 ttttgttcta ttcttcttct tccgccatga atgagaaacc catatcaaag aactataaag
      241 gttatcaaaa cagcgtttat gactaaatga attagagtta tctatcacta tttttcaagc
      301 gatttaaacc aaatggcata caatatggca tacaaaaact tcaataagcc gtgggggttt
      361 ttaaatttct tcttgttctt taaaatcaat gagtggagct acataatttg aatccacttt
      421 ttcaaacgtc actttttgca tcgacgaatg ttttctttcc caagataacg taaatagatt
      481 tgactctgga cttataaagt ctacttcacc ttctagtatt actttcttat gcaaatattc
      541 ctctaataaa tatagacttg ctaaagcata caacacattt tccaaatttg cttccttata
      601 atttgaagaa cgaccatgtt ttactctgtt atatattttc caccaatctg gtgtatcttc
      661 aggccaccca tctccaaaag gttttagtga agtcaatgtt aaagaattca taaccctaac
      721 accattatcc aaacacatta attcgctaga tttaataaca gctttaaaat cagttatgct
      781 tttattgcta ctgctatcaa atccgcaaag ttctttgaaa ataatctcac actccgaacc
      841 aatcgctagt agtagttttt gatattcaat agagaatgtt ttgaaattat tctcatcgag
      901 catcacaaaa tctgtggtat taataaactc attttctaaa tgtagataat acttccaata
      961 aaaatttgta aactctgctt tgtccattaa atcacctctt ttgaattata acaaaaaaaa
     1021 cacgtccaca acagacgtgt tctttgattt ctacacaatc agcttgccaa aaccatccaa
     1081 tcagtcctag catgcctgta ttaaaattga acgttcatca tctatcatct ttcttagtcc
     1141 agcatgtttg caaccacacc tacaattttc agtccaacaa tagatttcga ctagctaagt
     1201 cttgcaaaat tcaggcagct aacccttcat ttactacctt aattatacta aatacccaca
     1261 acgtttacaa gtgtaatcat tctaagaaat ctgtttagct atgatcgttg tataatcttt
     1321 aaacgccgtt ccttcattga ttgaaataat tttataactc ttgtttttcc aagctacttt
     1381 catatcgttt gttagttcat aatcttgatc gtatcgaata ataaacgtca acgttccttc
     1441 aagaatggta cctacactag caagcacgtc ttttagcatt tgtgaacgca cattagccca
     1501 acattcaaat acagtttctt cactatccac catctgccca tcttcatctt ttacccgttt
     1561 tgtcttttta aacttgactc tctcagtcaa ttcatttaca ttcttaatga ttggcataat
     1621 aagcacctct catttgttgt actaatgctt gtaccgttac tggtatccgt tcgctgctgg
     1681 cttcttgacg gttcaaatac caatgttgag taagtaatga caccgccaaa tcaaattgct
     1741 gataattatc catatcttta tccgttgcgc tactatcaat agcacttttt atatattcgc
     1801 ctgctgtatc aatcaattgt tgaataaagt catcatcaat agtgtgatcg atccgcatac
     1861 ttttttttat gctatccaaa attaccattt tatctctcct aatgaaaaaa aggcagtggg
     1921 ataaaccgcc gcccaatatt tttctatact tttggtgtta caactggcgt aaactcaata
     1981 tacactgaag cattttgatc aattttctta taatcgttgc gaacgatcac tgcaagtcct
     2041 tgtgagtagt aatcgaattt ttcccattga gtcgttactt gatttctacg agcaacaaag
     2101 atagattgtg caatatcccc cataatcata gggaatgttc cagctttagg attggcaaac
     2161 aatgtatctg caatcaatac tactggcata ccgaataatg atttaccact aggtgctgtc
     2221 acatcgggtt gtaaaatgta acgtccatct gaatctttca atgtatctag gtggtaatat
     2281 ccgctttggt ttagaattac cattttattt aatgctgggt ctaatgccac attgtatagt
     2341 tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga atgtttttaa taaatccatg
     2401 atatgcttat tgtcagtgtt ttctaccaat ttcgctaact gatctttgac ctcttgtaca
     2461 atattcactg ctgaatcttc cacaacctca tttgataagg caatcttacc agcacgagtt
     2521 tctactttat aatcaactga agtaaacatt tcagcgtcaa tatcaccaat ttcagcaagt
     2581 tcagcttttg ttgctaacac tgcttgttgg ttagttgcta ctggatattt accttgtcca
     2641 tttgatactg tttttactgt tgcatattga gctaagttat aatttgaacg tttcaaatca
     2701 aagacttcac cgattacttc ttcgggaaca actgccgctg catttacagt agtgactcca
     2761 tcacgcactt cgccttgtga acggatatat tcttcatacc cacgcaattc tgttttttcg
     2821 ttatcgatta aagttttttc catgttatta ctctcctttt gtgctttaga agtggattct
     2881 aagctattgt ttgtttgttt tgcttggttg ttactcataa acgattcgta agaacgctta
     2941 tttacttgga catttgatga atcataagcg ggaacggtca cgacactaat ttcattaagt
     3001 gctttgattt tatctattga tcgttcaatt gtgccatctt cttttttgtc gaatgaatct
     3061 aatccaagca cgaacccaaa ggacatagaa tcaacaaccc cttttgagat attctcatac
     3121 acatcatttg aataggtcgt gtcggttagc tctgcttcaa aatgtaatcc tgtatcatcg
     3181 acattcaatt tcaacgtacc tgctttgaca ctagctagtg gctttgaata atcgtgtcca
     3241 tataataaga acacatttga taaatccacc tcttttaatg cttcgggtgt aatcacttct
     3301 acaaagccgc ctaaatcttt tgacggttgc ccgaatttca acgcatagcc tgaaatagtt
     3361 ttgccgtctg ttggttgttt ttcattttct ttctctactt tgggagaatc ggctgttagc
     3421 tcagcttctt ctgtcagccg tttttccttt tcttcttcat tattcaactg gtgtaactcc
     3481 ttcctgttct tttaattgat atttctccaa cgtatctaaa tacgtaaagt tcaaacttgc
     3541 tagtaaacga tcgccaccat ccatgggggg tagccccatt ttcgaccgcc cctcattaat
     3601 cgtgagaagt gaccctttga cctgatccaa tacatttttt acttttgttt caggatcagt
     3661 ctctagtaac tgatcggaat taaagcggat attggtttca agtttcgtat ctaattcact
     3721 cacaaacaca ttaaagtaat gaatcagcgt actttggata tactggagat tactttgaac
     3781 ggtgctagaa tgttcatttt caacgcctaa acgctcaatc ggtacaccaa acgccttggc
     3841 aatttgtttt gtgttccaat cattggaatt gataagcttc aaaacatcgg tgtttatttc
     3901 taatgttttg tagtccattg tttcatcaag aatgatcgtt ctaagagcat tatctccact
     3961 acttgaaccg ttggcttctt caaacttttc acgaattgcg ttctttgctg atccgtctaa
     4021 atctgactta tgaaccttca aaatacctga cccactgaca ccacgagtaa agaaattgtg
     4081 caatgtgcgg ttgccagctt gttgaatttt cagttcatca cgtaacgcat aaagtggtgg
     4141 taatcctgtt aaaccatctt gggtgaaata tttgaagtgc aaaatatcgc tagacttcac
     4201 acgtcttttt ttatcaccaa tctgataaga caacgtacca ttatctaatt gagtaaccgt
     4261 tacacttgag ttaggtaata agtgaatttc ttctaccttt tcaccgttgc gtttaatctc
     4321 tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa gcaaacttta aatgccaacc
     4381 atccatttct gaattaggtt tctcgtttag taacttcacc agttcattat cagcttgtgg
     4441 catattgttt ttgaccaatt gaatcggact tgaagcaata tcactagcaa taattcgaac
     4501 ggctgcaaac acatcactat ttcttaatgc acgaacgctt gtaaagctgg tggtgtaatc
     4561 atccgattgg attgatacca catgttgtag gaaaggatct cccgttgtat tttgtctcgc
     4621 tttaaaaaat gccatctttt aacctccctt ctgttctgta tttagcatga ccgctacccc
     4681 aataagcact atgcccagcg ctaacaagcc atagtagaca tttgttagta aggtgatagc
     4741 aaccaacaca cacaccaatc caagaagtaa aagaacggta tgaatgtaat tagaaagaaa
     4801 attcttcact tgtataaaat tcattgtctg ctttcgctcc ttccgttgtg gtgaagtaat
     4861 ccattgcaaa cacataggca ttgattaatg cagctattgg atcaattttg ttactgttct
     4921 ttgatttatt aatctgaatg ccgttgttat ccgtttttaa aatagcgttg ttcactgcat
     4981 gggtgagaat cgtattttta ttgtgaataa cgttgccttc ataaacttgt tctctaaaag
     5041 ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac ttctaacatt gggtagttgg
     5101 ctttttcagc tttactgata agcgaattag cgttatacgg gtcataacaa atccccatac
     5161 attccaaatc attttcttga atcaaatctt gaataaactg aaatacttgg tcatagtcca
     5221 cgattccact ttctaattga gtaatggaac actcaccagc tttttctagt tctcgataag
     5281 gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt ggtggctaca aagctatgtg
     5341 aatcacaata aagttcgccg ttatctagcg gtacgatcca tgaaacgcta gttaaatcat
     5401 ctgttttaga taaatccact ccgatataaa cgggcttgcc tgtaatatct tgtggttcta
     5461 cttcggttgc ctgccagtct tcagcaatca tatagctgtc ctcactcgct tgtctccata
     5521 aattgaagtt tttcaccaat acagaattaa ggttattttg tttcaaagca aggttcacat
     5581 catcttgaat ggttggaatc attacttttt tgatttcttc actttcaaaa atcggattgg
     5641 ctttgatcca tttctcttga tcgtgaattt cttcgggatc gtccaattcc catatcgcta
     5701 taaagtacct gtcggcttgt tcttcgcctt ttagcacacg atctaacaat aagtattcct
     5761 cgtacatagg aacgtttaaa tcaagcccag cggtactaat aacacacaat aagccgttct
     5821 tttgttgggt cattcctgac ttaatgacgt tgtacgtttt acgagttttt gcttcgtgcc
     5881 actcgtcaag aattgcggtt gttggtgcaa acccatctag cgtgctggtt tcactagcca
     5941 gtgccatagc gaacgaatta gagggcaagt gctgaatctt agagttcata attttcagtt
     6001 gtggtcttaa aaacttacta gacttgacca cgtttctaag tgaattagac agcatatcat
     6061 agcccaattt agcttgtttc aaagcgttac ttacaaacaa tacttgcctt gcttctgctg
     6121 gttcttgttc cataatcaac gcattggcag ccatgccact tgcgatatac gttttcccat
     6181 tcttacggct catactggta aacgctcgat tgaatcgcct gtaattccct tctttagtac
     6241 gccagccata aagactgcct agaatgaatt tttgaaatcc taacatcttg attgctttac
     6301 cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt ggtggcttgt tcttcatcga
     6361 acacataagg gaagctatct gacttagaac gctccaaatc gcttatatga cgctcacacg
     6421 cttggataat tttcttgggt gcaatcagct tacctgatag gactttctca atgtaatcat
     6481 tcatgaattc atcaactctt tgaacgggtc ttcgggttca tcatttttaa ccttgttcaa
     6541 agctagtttt gatcgtgatt ctaaggtcat acctagtgag gtggcttgtt ttttcaaatc
     6601 actcatagct tgtgattgaa cccgaaccgc tggattggct ttctttactc cgttttcgca
     6661 tgtgataaac gtacctgatt ttcttatctc ggcttcggct gttttcattc gagcaaaggc
     6721 taaacaatag gctaccaatg aaccataatc tgcttcactc aatggataat ctttttttaa
     6781 tgttggtacg atacgattcc attcacttct agcgctcgct gccatccagt cagggggcgt
     6841 gatatttatt aatggttgct gcttgaacaa ttcttctcta gcgtccacac ggattgcttt
     6901 ttcttcactt gatatattgc cttttgtatc gtgtaatatt ttagggtgtc ccatgttgtc
     6961 ctcctttcag acaaaagaaa aaggaacgta agcaattaag ctcacattcc ttcgtttttc
     7021 gatcagaatt tttctttatc taatttatct atataaatga tttttccgtt ctgcatttga
     7081 atggtcactt gaccaaaatg aggtaactca atggtgcgct gtttccctgt tttatcagta
     7141 ataacaattg tatttttcaa taaatctcct actttcatta tatacttttt cacttgtgat
     7201 tacaagtgta atcgttattt gtaattaccc ttatgccttt tcacaacggt tatttgtacc
     7261 aaaatgagta ggcatcgatt gtcagtaagt ttcacactat gcccccttaa ctcaaagggt
     7321 actcttttct cgtctttgtc tttcattggc tgtcttggaa ttatggtgtg catggcataa
     7381 agaacgaaaa ttagtttcat ctaaccgtct attccaatca tcttttagtt caattttgtg
     7441 atctacaaca tcagctttcc ttatcaagcc ttcttctagg caatcctcac atactggatg
     7501 atttaaccga tacaagtatg aagcctttcg ccatgttcta ctttgataga attgaaaata
     7561 tttaccttcg ctttgctttc tttcttcacg cttaattgcc gtttgctttg ctttatgctt
     7621 cccacaatac ttctgcctat aatcaatcaa tgttttacat ccagcatgat tacattgttt
     7681 tttaactgcc atagattacc aaccaaagcg atctcgttcg tcagaatcat cttgttccca
     7741 ttttgatttg tattcaatat aaggcgaaac tatcttcaag cacgttccgt ctttaaaatt
     7801 aactttagtg attttatctt cctcaaaact aataaagtct acatcttctt gagtgtttga
     7861 atactcaatg tgtttctcac catcgaagta tctaatactt tccatatgca taattattct
     7921 acctccaata aattagttat caattcatca tctgttagta gcaaagccaa gaacttatca
     7981 tggatgttta aaaacaaatt atcctcattc gatgtaagct ctcgttgtct cgctcgatca
     8041 tataggttaa gcagacaatt aaattcatcc attagctcac ctgtatcatc atagttgcca
     8101 ctatctacca atgatcccac tactagcgat tgacaccacg ctactgactc ctgcagccaa
     8161 tcaggttctt cttccattat ctttaatact tctggatcaa atgtggaata atcttctgtt
     8221 accaatccca cactaaatgc cagcgtgttc attagaaacc gatccatttc atctgtatat
     8281 tgaaagttcg gtgctttgtt tgttgaaata atattgagca tttactttcc tccttgattc
     8341 gtaacacgtg gtaacaccta cgtaacaggt acccgttacc actcaatccc aacggggaca
     8401 agggttctag cgttttggta acaggtaacg ggtaaaatcc agtaacatta ttccccagca
     8461 tattagctaa ctatttcaaa agttttaagg aaataaggtg ttaccccgtt accaaattgc
     8521 tgtatccctt ggggcagtaa gggtaaacta gtaacgggct aggtgttact gacccgttac
     8581 tacccgttac tttttgtact ttccagctcg atttttattt tcataggcat ttgacttatt
     8641 gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc cgttcccaac caggaatact
     8701 gctaataatc aaggcaatct ttttagtttc tgcatctgat cgaccattca ataaatcctt
     8761 tgcttgttta tcaaaaacag cttccaaaat atcagcggtg agtacgctat caactaaata
     8821 aaactcttgt tgcatagtag ctccattata gaaatgctgt aaatttttct cattacattt
     8881 actgtcagga tagttctgat aataagaacg tctcgcccac gctggagctt cttcccattt
     8941 atacgggatt tccatttcca aatatttagt gattgcttct ttaattggat tctccatctt
     9001 ggcatcctct tgatagtctt ttgcaatttc taagacttct ttatcttcat cgggactaaa
     9061 atagattcgt tgtcctttat caaaaagcac ctttgcttca gctaacactt gaagaaaata
     9121 ttcatccttt gttttaaacg gattcatctt tggcttattc ttacaaggca aaggataaaa
     9181 acggcggttt cctgtatcat ctttcaaata ctgcccatca ttcgacgttc caataaatac
     9241 acattgcctt gcccaagcta tgacattccg accataagga ggtctaattt tatcgatctt
     9301 ggcactgatg aagtttttta tctgttcaat tttagttttg ttcattgaag ataattctcc
     9361 aagttcaata attacattgc cttgaagctg catataatca tctttctttt cacctagcga
     9421 atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat gctggaacgg tactcttacc
     9481 aatcccttgc ggtccatcca aaactggaac gatctcaaac tttattcctg gtttatagat
     9541 acgtgcgact gatccgacta tccaacgttt tgttacttct cgtgtgtaaa ggttgtcgtc
     9601 tacccctaaa aggtcaataa acagtgtttc cagtctaggc tgttgatccc atttaacttt
     9661 ttcgatacgt tcttttactg ggttgaaaac attctttcta gccacattta ctactgcttt
     9721 gtgtattttg ttgtcatcaa aaactacatt gtagttttct tcaatatatg ccgttaaggc
     9781 atcctcaaaa ctgtcttccc attctccagc ggtacgattg atccaaggag aatcttttaa
     9841 taggtgaatg tatccgctaa attcgttata agcaattgtt ccagctaaat tgctatcatt
     9901 ggttaaaatc aattctgcat tcacaacact gttactttta atctgatcgt ctttattgca
     9961 tcttaatcgt gacatccagg ttttcaattc aatgatattg tcttcacgct cgctggcttc
    10021 gggaaagtct aaaatactat ctaatttatg cttatgagcc atgagctttc cccctcaaat
    10081 cttttttaat catcgatcga ataatttgat tcacttcttt atcttttagc ggtggttcat
    10141 tgtacgcatt tgctgtataa atcaattctg ctaaagtttg attatctaca ccgtgccata
    10201 gtaaccaacc gcataactta gttaaatgat cgtttctact accttcgcca gcgccttgaa
    10261 caatcatatc taattttttt gctgtccctg tttttgctac gggtcggtat tgagtcttta
    10321 tttcttgcgg tttttttagc agtaggttca ctagccaatc gggagctggt gcaatattat
    10381 ttagagatac gttttcaata ggttcatatg ctctcccaac acttcttgaa ataatcatag
    10441 ttggcgctat tggaactcct ttagtgataa tatcaatccc gcttaaactt ttacctttat
    10501 catcacgccc gaagatatca gcaaatgcac ctgtttcttg ttggataatt acaccatcag
    10561 gaattttaaa gaagaaatgg attcctttac ctggtgtgat ttccgtataa gtagaaggaa
    10621 atggatcata cattgaagca agttttttat aattctcaac gccgtttcgt gtttcagaat
    10681 gttgatctag gtcaactacc accatttgat gatcttcaag cattaaccca atatttctag
    10741 ttgcatcttc cgtccaccat tcattaattt gattagaatc gttagttgca tcattccaac
    10801 cactagagcc ttttattgga attttagttc caggagataa gggataaatt gaccatcccg
    10861 acttttgtaa ttccgttgct gcttgataat tatttgccaa taaacctctc ctcctttttc
    10921 aaaatactgg tataaatcat tcagacacct ccatgagctt caactgctca agttcgtatt
    10981 tcaacccatc ccgaacaaat tcagcacctt ttaattgatt ctgatacttt acccatgtat
    11041 taaatgtttt cacccttgct tcttgcatat cagtaatgtt taaccatgcg gttaaaatct
    11101 gattttttag ctcgttgatt tctcgacaaa ttattttgtg ttctgcatgt tttttgagta
    11161 catacaaaac tgttagtttt tcattgaatg aatttccaaa gtaggttttc ccgatattat
    11221 tttctagttc agcgatccct tgattaattt cctctaattc aaggctatcg agttggttta
    11281 atagttttgt tttgtacatc tacttatgcc ctccagttct taattgatag tcgtactcgt
    11341 cataagcagt gaagagatat aacgctaatc ctgcaaagat agctattttt gcaattaatg
    11401 gtaataagcc gaaaattccg ccgattaaaa tgcttcctat aattaagttt cgtttcataa
    11461 tggttccctc cgatttttta atatggtata atcggaacaa agaatatcgg gttttatccc
    11521 aattaagttg cctttatcct gtccgccaag actgttatgg ataaggcttt ttctttgttc
    11581 tcgatcatgt taatgctcct aagttgtctg caattcagca gaacgattat aaattttctc
    11641 actcactgaa aactgcaacc catatttttc tttaacagtc attaattcta ctgtctcatc
    11701 taaaataggt tcacgatcaa ccaacatttc aggagtcata tctttctttt ttaccatttt
    11761 ggaatgacca taaatagttg atacagcttt attggctatc acattcgctt taatgtagtc
    11821 tactggtttt ggttcagtaa tagcgtggct taatttagtc attgcttctt tttggtgttc
    11881 tttgtctagc atgcggaata cttgaaagcc ttcaagtcct gtggactggc gtagttcttt
    11941 gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct tttttacggc ttttgaatgc
    12001 catgcggtaa atattttttt tcgttgatga tgtttacatc ttgtgttctg cccaatgaat
    12061 cgatgacctt actagtagta accccatctt taggcaaaga gtgaatggct ctagtaacct
    12121 gtttaagacc taacgcttcc gcaacatctt tcgccactgc ccaccattca tcattaatat
    12181 cgacaaatct tataatgtgt ccgttccaaa tttcagtttt cattcaacgt cctccagttc
    12241 aagccctagc aagctcgcta acaactcaac ttgctgatac aaaatttctt gatactcttc
    12301 aatagaaagt cgttctttaa actctgcttg tactaaatcg ttcaagccac gcaatactcc
    12361 gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc atttgttcgt acaaacattc
    12421 aaacgtttct tctagcgctt tgccttccaa gtttgctaat tctgccgctg tgtcttctgt
    12481 aagaattgtt gataatactt tttcgattgc tttattttgt gtcgattcca ttattatttc
    12541 ctcctattaa tccctttgcc cccacggctt gcgtttattt ttggtattct tccataaagc
    12601 gatcacattc atcttgtgaa attcttacga ctccatcaac tataatcaac tttaaaccgt
    12661 taggaacata tttctgcatc atagtattgt atgaaacgtt catatactta gcagcttgtg
    12721 ccttgttcat atattttgga aactggtact ttttttctaa taatgaaagt aacgttcctt
    12781 gagtttgctc tacttgttga acaactcgat cgtcaataat tttaaaagct ggatcatcaa
    12841 gcgtgagttt gtcaattttt aattccatat ttatcccccc tcgttagcat acatttctat
    12901 acaaagattt taaaaaaatg caagaggctc ttgaatacat ttctatacct cttataaact
    12961 caatatacca tcataccttt tcatttgcaa gaattttaac atactttttt gaacttttat
    13021 gtttcacaca tatttaaatc gtgatatagt agagaaaata tatgaatggt ggtgaaaatg
    13081 tggctagaaa aaaatacccc gaagcagtgg agacaggaca aagaattaga ctggctagaa
    13141 ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt atccccaccg gccagtaaag
    13201 gtgccgttag taattgggaa aatggctata atcttcccaa taacaccagg cttaaacaac
    13261 tttcaaaggt attagatgta tccactacct atctattaaa cggatcttat acattaagag
    13321 atatgcatat gatgcccgaa attaagcgaa atgtgcttga aaagcaattg aaaaaaacta
    13381 ttgaaaaaaa taactctata attgattcta atctttctaa tgctatcaat aacatcgaag
    13441 aagataacct gactgtctca cagaagattt atttaacaaa cgcaataaaa tatcttaaaa
    13501 caggatctcg aattgaattt cctgacggga gtaccccaat tgcttatctc gcagctcttc
    13561 taaatacgct tttacgatat gactcattaa ttaatgattc agaaaaaaat acagatgaga
    13621 aaaaagagta tcaagaattt tatgaaaatg atcttctaaa agagttcaca gctcttgtta
    13681 ctgagctaaa taaacactat tcaaaataat aataaactgc ctcctccaac cgcccccacg
    13741 gcttgctaca ctatggaggg aaaactatta tggcaacatt caagcaatat gaaactaaaa
    13801 aaggtaaatt ttggctctat gaagcctatc ttggaatgaa caaaatgact ggcaaacctg
    13861 ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc ccagcttgcc ttgtcacgct
    13921 tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa caacgaaacg tttaaagaag
    13981 tttatgactt gtggctagaa acatataaaa ccactgtccg agaagccacc ttcatgaaaa
    14041 ctgaaatcaa gtttagaaag tggattcttc caaaatatgg aaatcttcgt gtgaatgaag
    14101 tcacggtaaa aaaagcccaa caaattgtaa atagctgggc tgaaactact gaccaataca
    14161 aggtacttca ttcaacttct aagcgtattt ttagctacgc tatcaatctt gggattattg
    14221 agactaatcc actagacaac gtattaatgc ctaaaagaac caccactaag cataaagaaa
    14281 aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt tgattaccta aacagcaaac
    14341 ctgaaactta tcgaaacgac tttgataaaa cgttactacg ctttctcttt tatagcgggg
    14401 ttaggatcag tgaagccctt gcattgaatt ggtcagacat tgattttcaa gaaaatacgg
    14461 tcaccatcaa taaaacacta agccaatcca aaaatggcta taaaatcagt gatcctaaaa
    14521 ctgattgtag tgctggggtt cttccactag atgaaagaac aatttctata ttaaaaaaat
    14581 ggcagattaa tcagcggaaa tatatgctga cacttggtgt aactgatccg actatgattt
    14641 tttgtggtat ctataaagaa ctaatcacac accacgctat ttatgcaaga ttgattacta
    14701 tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt agcaagacac actcatgctt
    14761 caatgctctt ggacgctgga gctactatgg tagaagttca aactagatta cggcatacaa
    14821 aaattagtca gacgatcgat tgttacggtc acttggcaaa agaaacgaaa gaaaagactg
    14881 ttgaaaaatt agtacaacac ttaaatattg actagatggc atacaatatg gcatacagta
    14941 aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc tcctagcttt atagtctatt
    15001 cttcttcttc cgccatgaat gtattgaata cttcttcaat catgtcccat tctgcgtctg
    15061 tttcgatttg ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt
    15121 gtaattctac atcttcatct tctgaaacac cagcaggata taaaagaaca tagttacgtc
    15181 caaattcttc ttgtccgtca actgttagta ggatttcata taatgtttca tttccttggt
    15241 catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg ggaatggtta tgttctgtca
    15301 taagattacc tcttttcttt tattattaaa ataattgctt acctgagccg tctaaataat
    15361 tttgtaagat catcacagct gctaatttat cgatgacttt ttttcgtttg gctcttgaag
    15421 tatttgcctg ctctactagc atacgttctg cctgaacagt ggttaatcgt tcatcttgat
    15481 aatcaactgg caaggagaat agttctttga tcttttctcc gtatgacatg gaagcttctg
    15541 cccttggtcc gatggaattg ttcatatttt taggtaatcc aactacaaac cggtctactt
    15601 catattgtgc tactaattcc ttcaaccgat caaagccaaa ctcttcttct gcttcattga
    15661 tccgaatgat ctctactcct tgagctgtcc agccaaaggg gtcactgata gcaacaccta
    15721 ctgtcttaga accgacatcc aagcccatta ctctcataaa ttcactccat gatttgagag
    15781 gtagtccttg acaattactt ccatgatctc atcgcgttcg tgacggcgaa tcaaatttcg
    15841 agcatcacga taacgaggaa tgtaggccgg gtctccagaa agcaagtagc cgacaatctg
    15901 attgattgga ttgtagcctt tttcttccaa agcccgataa acaatcgcta atgtttcgct
    15961 cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg gtttcatctg taaaacccat
    16021 gattctacac ctctttcccg caaggtaatt ccagcttacc ttctacaacc ttattttact
    16081 cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc gaataaatta agaaacacta
    16141 tttgtatgtt tttttccttt tttctgtagc tttatcaaaa gtactctttt cataaagtat
    16201 acgctatatt tctttgaatg tatgttttta cacaattttt tcataatatt cataaaaaat
    16261 tcgaatagct gctactaatt ttttgttttt gttgcttgtc ctgtctcata attgatcgtg
    16321 tagccatctt gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt
    16381 tctgcttcca attgcactcc cctggctacc agttcattcc cctcaaaatg aggagtgaca
    16441 cggtagcgta catgatgatt tgtttctttg atatatgccg caatatcatt ttcgtgagca
    16501 agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca agtttttaat gttgttattt
    16561 tgaccagtca gctggaaacc aattaaatgg ctgcgattat ataaccattg cccgtcactt
    16621 aatttctttt gtttccaacc tgtcgggtca atatacaaag gctctctctt ttcagtaggg
    16681 aacaaatctt ttccaagcat agcattggct tgtcctactc tgttcaattt atcaatattt
    16741 gaatagcttt cccatgttcc tttggaaagg ctaagatcct cttttgtaaa ttccggctga
    16801 ttcccattga tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga
    16861 gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct gaaagatatc tgaatctttt
    16921 tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag cgacgatccc cacgatcgcc
    16981 ttggttgttt ttttgctcat ttctttactc ctttactaac agctccctaa atcttaacat
    17041 atgttcccct ataaataaat actagaaaga aatagaaaca gcaaaaaata aaacctgttt
    17101 taagatggct taaaaataca aaaaagagac ggattcctcg tctctaatca gtcaccactc
    17161 tatctgtaag gtatttatta ttattcaaaa attttcttca tactcaagac aatacactga
    17221 tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat ctgcggtaat aagtccatcc
    17281 aagttaaaaa cataagaagt tgtttttgct tggctgttct tattacttgt tgcagagcag
    17341 ctttttcaca acctatttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga
    17401 agctgggatt ttaccactaa actacacccg caatacaaaa acagtatacg cctcttttat
    17461 tccttcctca agttttttta ttctttacgc ttctggtatg ttttgtagta aaataaaact
    17521 aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac
    17581 acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa
    17641 gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact
    17701 caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc
    17761 aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt
    17821 gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt
    17881 tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg
    17941 gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga
    18001 tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta
    18061 tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat
    18121 caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac
    18181 gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg
    18241 atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag
    18301 gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact
    18361 gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct
    18421 ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt
    18481 ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg
    18541 aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta
    18601 gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg
    18661 gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt
    18721 aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa
    18781 cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt
    18841 gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt
    18901 aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg
    18961 acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca
    19021 tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat
    19081 aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc
    19141 aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca
    19201 atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta aataattggt
    19261 taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca
    19321 ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct
    19381 gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc
    19441 cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca
    19501 gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta
    19561 agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag
    19621 ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg
    19681 aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa
    19741 ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt
    19801 ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa
    19861 atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct
    19921 gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat
    19981 tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg
    20041 agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag
    20101 ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca
    20161 aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa
    20221 taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct
    20281 ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc
    20341 cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa
    20401 gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa
    20461 atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag
    20521 ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat
    20581 ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct
    20641 gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca
    20701 ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt
    20761 ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct
    20821 tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct
    20881 tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc
    20941 gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata
    21001 tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag
    21061 gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa
    21121 tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca
    21181 tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt
    21241 gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga
    21301 gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc
    21361 atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt
    21421 aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt
    21481 gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta
    21541 aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt
    21601 tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga
    21661 tgtaatgttt tcagtattaa tgatataagc ttttaaatct ttacataaat actcatattc
    21721 gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat ctttcaatac
    21781 taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt catcgtaatg
    21841 gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg tagcatgttg
    21901 aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt tatttttttc
    21961 ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa taacagttat
    22021 tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct tttctctaag
    22081 catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta ggaaaaaaat
    22141 atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa cgttcaatcc
    22201 ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca aaatttctac
    22261 tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta ccataaaaag
    22321 tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa catacatgtt
    22381 aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc gatatcaaat
    22441 actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca tgcaaaaaaa
    22501 gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt attttgttct
    22561 tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca gcgtttgtaa
    22621 caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg atggttcctt
    22681 ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc gtcaacaaac
    22741 caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag aagatatctg
    22801 tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt ttgccaatga
    22861 attcgctagg gtaattataa attagctcca taacttctaa atagttttca tctgtgatct
    22921 tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct aagtagtctg
    22981 atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc tccggatccc
    23041 ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc gtagtgtcga
    23101 gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt acataagcga
    23161 ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt gccttttctt
    23221 ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga gcagataaaa
    23281 tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg agtttgccag
    23341 aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac cgcagcatat
    23401 tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag tccgatcagc
    23461 gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt gatatcgacc
    23521 attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact taacaaagaa
    23581 gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa taacagcatg
    23641 atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt tggtaaataa
    23701 gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt atcaaaaaat
    23761 tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt ttttggttca
    23821 ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc tactttagac
    23881 gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac accaatcaca
    23941 agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga attgccaaat
    24001 gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag cataaaagca
    24061 aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg tacgatgccg
    24121 cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga gaagatacga
    24181 ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa ggcgcctgag
    24241 ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa gaaaatcgta
    24301 ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct ttttatttct
    24361 agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc tctgtaaatt
    24421 tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt
    24481 aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac
    24541 cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct
    24601 ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac
    24661 acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa
    24721 atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg
    24781 gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga
    24841 agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa
    24901 aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat
    24961 tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc
    25021 aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca
    25081 gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt
    25141 gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct
    25201 gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca
    25261 ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt
    25321 aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt
    25381 aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct
    25441 aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca
    25501 cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga
    25561 taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga
    25621 cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa
    25681 aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt atttatcatt
    25741 tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt
    25801 tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc
    25861 agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta
    25921 caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga
    25981 tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc
    26041 tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc
    26101 tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca
    26161 ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc
    26221 tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat
    26281 tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg
    26341 gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc
    26401 tagatgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc
    26461 aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag
    26521 tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga
    26581 aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc
    26641 aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca
    26701 caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc
    26761 aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag
    26821 tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca
    26881 atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat
    26941 cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag
    27001 ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga
    27061 cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg
    27121 ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg
    27181 gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga
    27241 ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat
    27301 tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga
    27361 tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg
    27421 attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg gcacaagatg
    27481 agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc aaagaacagc
    27541 aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa atggcgcgga
    27601 tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac ctggaagatc
    27661 acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat caagaaacaa
    27721 aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat taacgaaaaa
    27781 accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta tcaaacaaaa
    27841 attccatatt tacctttttc accaataaga agaccaatta ctagtcattc tcactaaagc
    27901 ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat ttttgggctt
    27961 attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat atctttcttt
    28021 caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg ttttgtctaa
    28081 tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac tagatgtatc
    28141 tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg tatcttctgg
    28201 caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt tttgttcttt
    28261 caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc tagcccaaca
    28321 ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat ctttgacatc
    28381 catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg ctgcattttg
    28441 aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac tcgcccaata
    28501 caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg aaggatcact
    28561 aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa gtaatgtttc
    28621 tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat aatctgctac
    28681 ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt gacagtcaat
    28741 cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag gatcacccgt
    28801 cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac caatattgtc
    28861 tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact gactgttact
    28921 gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat tattccattc
    28981 tttttccatt aattctttat aacattcatt tatccatttt tctggatgta ttatctcttc
    29041 aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag tcttttttta
    29101 ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt gggacgattg
    29161 ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt tccgagccaa
    29221 tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc ccatgtcatc
    29281 aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt atcgtcactt
    29341 aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt gtcttcttct
    29401 atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc atatagaaca
    29461 tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa aggaataagc
    29521 caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac ctctttctta
    29581 catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt gatgctctca
    29641 ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat tgaatccatt
    29701 gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg actagaataa
    29761 gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa ttgtcccgca
    29821 ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc tgaaagaagt
    29881 ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc atggtcttca
    29941 ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt actattatag
    30001 ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc tttttcatca
    30061 aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt gatttcacct
    30121 aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga aactgcaaaa
    30181 cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa aaaatgaata
    30241 tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg cttttctggt
    30301 tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata ataatcttgg
    30361 cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc tatattatca
    30421 aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat aattgttccg
    30481 acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa aatttgcgag
    30541 aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa taattgttca
    30601 tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc tctttcatgt
    30661 atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc tgatagaatt
    30721 aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca taaaacccga
    30781 gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg tatataatca
    30841 atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt tttaaataag
    30901 tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt attttctcct
    30961 ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct tcaataaaaa
    31021 aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat gcttctgctc
    31081 cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt tctgtctttt
    31141 ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa acatatcctg
    31201 ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata ttcgaatctt
    31261 ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga ataaaattta
    31321 ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga taacttaacg
    31381 cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca ttgtatcctt
    31441 gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg atatgcccgg
    31501 ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc cgagcaattt
    31561 tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt aaattcatca
    31621 tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa tagttccctg
    31681 aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt cgactatgtt
    31741 tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa cctacatcat
    31801 gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt tgtattgctg
    31861 cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata ccactccaaa
    31921 aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat tttccgttgt
    31981 aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc ttttcagaag
    32041 ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat atcccgccat
    32101 tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt taacattcgt
    32161 tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga ctgcgcccac
    32221 acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat tgcgccataa
    32281 ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg aaccgtatat
    32341 ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact tcccataaag
    32401 ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat tttcgtaaaa
    32461 atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt aggaaattga
    32521 ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg gatgatcaca
    32581 cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg aatcaatgtg
    32641 gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt attccccata
    32701 aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc taaaactgta
    32761 ctgattacag caataacaat cgaattccaa ataatcaagg gataattata tctcgccatg
    32821 atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc gccacttaaa
    32881 atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat caaaaatatt
    32941 aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt ttgtttagtc
    33001 atttttatca cctccgaatt taaattgaat agccgataat ataacagata caaacaccat
    33061 cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg ttttatataa
    33121 attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa agaatatatt
    33181 tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca ttaaaatgat
    33241 tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg ctccatctat
    33301 ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa ataaaatagt
    33361 gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta tccaaaaagg
    33421 atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat tgaataaccc
    33481 agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg taatggttgg
    33541 aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat tcaataaaac
    33601 agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga taatcaaaac
    33661 attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat tgtcaaatcc
    33721 aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga taatgatggg
    33781 aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat aagcactttt
    33841 tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc ttggtaacga
    33901 agattgcatc atcgttaagt cctcctaata ttttttaatc accagttaat tttacgccgt
    33961 acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca cctgtttgcc
    34021 aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg gctggtaaaa
    34081 cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt tgtcctcctt
    34141 agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta gatgtagcca
    34201 cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat gtaagaaatt
    34261 gttgtgccac ttgtggattt ttggcatctt tagggacata aaaacttgat cctcctgata
    34321 ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg cgccatttgt
    34381 cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca gcaacaaaca
    34441 ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt acaatatctg
    34501 agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta ttcagacgga
    34561 tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg ataaacaatt
    34621 cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct tttaatgctt
    34681 ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca cctatttcag
    34741 caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc caaggaataa
    34801 tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa aactgatcgc
    34861 catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga aatttttcca
    34921 gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct tcgtgtgtta
    34981 ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc acaattttta
    35041 agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca acatagtttg
    35101 tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc attggatctg
    35161 cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg aataataggg
    35221 taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc ccttctttct
    35281 tttcaagttc ttatataaaa cgctttcact tatgtatatt aacggaatta gtaacacaaa
    35341 tatttatata aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt tataatagtc
    35401 attaattata aatagaaagc tttttatata aaaagagaaa ggaaagtggt tcttttgtta
    35461 acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa cctattccat agatacccat
    35521 cttcatttat ctgctgctcc ctattttcga ctactaataa atgaaagcgc taatttatat
    35581 gctgaatttg cacataaatc tatagaacta gataatcctt cccttttatt cattgataaa
    35641 cagcaagaag tccaaatctt ttctactcaa aaggaaacac aaacttttat cattatccag
    35701 ttttattttg atgagcaatt attagcacat attacgggac aatctttttc tatagatcaa
    35761 gaaataacat ctatcattac ttttctgaaa aatgaacagt tcaaaattga ttatctaaaa
    35821 catagtaagc cagctatttc ctatgatcag gaaatcaatt cttttttcat actatgccaa
    35881 cagatgagca ttcatttgct ttccttatta gttacttatt gtctgcaaaa aattcaattg
    35941 tcttccttac caaggcatta tcaacaggaa cttgctttat tgacaatacc ttcaaatgag
    36001 aaagtcattc ctaaaaaaaa gcatattctt tattcagatg aattagttga tccaatcata
    36061 cgatatttag aagaacatct ttcagaaaat tttttgcttg aacaattagc ccaaattttt
    36121 tttatcagtc cagccagctt aaaaaaacat tttagaaaag tgaatggaaa aagtgttatt
    36181 agctatttta aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga aaatgaatta
    36241 acctatacag aaattgcaga tatattagga tttcattcga tccatcattt ttcttcagct
    36301 tttaaaaaat atacagggtt atccccttca aagtattatc tatccttaaa atagaaaaaa
    36361 atttttaggt tttatccaca aattatttct ctatattttc tgtctattca aaataaacaa
    36421 taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat caacgtagaa
    36481 aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt ccttcatttg
    36541 ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa cgcggagaaa
    36601 atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc actgaccgtg
    36661 actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc gatgtcttta
    36721 ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt aaagacctat
    36781 ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct ggtgctagac
    36841 cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc ctccgtagtg
    36901 tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa catgtcgtca
    36961 ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat cgcggcttag
    37021 acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca gaaattgcta
    37081 atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa acaagagtta
    37141 ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc ctttctgctg
    37201 atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa gcaaccggca
    37261 tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc aatctgccgg
    37321 acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt cagccgatcc
    37381 aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat attttaaatg
    37441 gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc tttgatcata
    37501 cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata acaaattata
    37561 aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct acaagattaa
    37621 atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa gcatttggag
    37681 aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga aacttgacag
    37741 tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact acccgtgacc
    37801 cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta gaaacgatca
    37861 aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat atacgtgaac
    37921 ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg aatgagttac
    37981 gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc agaagaggat
    38041 tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg attttgattg
    38101 aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt tacttgagga
    38161 gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata gaagataaat
    38221 gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa
    38281 cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg
    38341 agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat
    38401 catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat
    38461 ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga
    38521 tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct
    38581 cgttgtcttc taat
//
LOCUS       DACEND010000010        36560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEND010000010 DACEND010000000
VERSION     DACEND010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.011"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..745
                     /locus_tag="IW746_000459"
     CDS             98..745
                     /locus_tag="IW746_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1077387.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1138..3783
                     /locus_tag="IW746_000460"
     CDS             1138..3783
                     /locus_tag="IW746_000460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1077388.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4093..5406
                     /locus_tag="IW746_000461"
     CDS             4093..5406
                     /locus_tag="IW746_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1077389.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5396..6049
                     /locus_tag="IW746_000462"
     CDS             5396..6049
                     /locus_tag="IW746_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1077390.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6104..6775
                     /gene="radC"
                     /locus_tag="IW746_000463"
     CDS             6104..6775
                     /gene="radC"
                     /locus_tag="IW746_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1077391.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6670..8022)
                     /locus_tag="IW746_000464"
     CDS             complement(6670..8022)
                     /locus_tag="IW746_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1077392.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8088..9377)
                     /locus_tag="IW746_000465"
     CDS             complement(8088..9377)
                     /locus_tag="IW746_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1077393.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9464..10168)
                     /locus_tag="IW746_000466"
     CDS             complement(9464..10168)
                     /locus_tag="IW746_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1077394.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10368..10634
                     /locus_tag="IW746_000467"
     CDS             10368..10634
                     /locus_tag="IW746_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077395.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10802..11032)
                     /locus_tag="IW746_000468"
     CDS             complement(10802..11032)
                     /locus_tag="IW746_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1077396.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11104..11259
                     /locus_tag="IW746_000469"
     CDS             11104..11259
                     /locus_tag="IW746_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077397.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11568..11792)
                     /locus_tag="IW746_000470"
     CDS             complement(11568..11792)
                     /locus_tag="IW746_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1077398.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12059..12394
                     /locus_tag="IW746_000471"
     CDS             12059..12394
                     /locus_tag="IW746_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077399.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12627..13568
                     /locus_tag="IW746_000472"
     CDS             12627..13568
                     /locus_tag="IW746_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1077400.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13570..14460
                     /locus_tag="IW746_000473"
     CDS             13570..14460
                     /locus_tag="IW746_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1077401.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14503..15303
                     /locus_tag="IW746_000474"
     CDS             14503..15303
                     /locus_tag="IW746_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1077402.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15318..16169
                     /locus_tag="IW746_000475"
     CDS             15318..16169
                     /locus_tag="IW746_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1077403.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16166..16525
                     /locus_tag="IW746_000476"
     CDS             16166..16525
                     /locus_tag="IW746_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1077404.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16540..16701
                     /locus_tag="IW746_000477"
     CDS             16540..16701
                     /locus_tag="IW746_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1077405.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16698..17024
                     /locus_tag="IW746_000478"
     CDS             16698..17024
                     /locus_tag="IW746_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077406.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17021..17836
                     /locus_tag="IW746_000479"
     CDS             17021..17836
                     /locus_tag="IW746_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1077407.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17975..18286
                     /locus_tag="IW746_000480"
     CDS             17975..18286
                     /locus_tag="IW746_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077408.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18283..18513
                     /locus_tag="IW746_000481"
     CDS             18283..18513
                     /locus_tag="IW746_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077409.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            18520..18723
                     /locus_tag="IW746_000482"
     CDS             18520..18723
                     /locus_tag="IW746_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1077410.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18720..19019
                     /locus_tag="IW746_000483"
     CDS             18720..19019
                     /locus_tag="IW746_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077411.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19262..19675
                     /locus_tag="IW746_000484"
     CDS             19262..19675
                     /locus_tag="IW746_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1077412.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            19688..20074
                     /locus_tag="IW746_000485"
     CDS             19688..20074
                     /locus_tag="IW746_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077413.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            20625..20849
                     /locus_tag="IW746_000486"
     CDS             20625..20849
                     /locus_tag="IW746_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077414.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            20867..21295
                     /locus_tag="IW746_000487"
     CDS             20867..21295
                     /locus_tag="IW746_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077415.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            21273..22691
                     /gene="terL"
                     /locus_tag="IW746_000488"
     CDS             21273..22691
                     /gene="terL"
                     /locus_tag="IW746_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1077416.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            22703..24238
                     /locus_tag="IW746_000489"
     CDS             22703..24238
                     /locus_tag="IW746_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1077417.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLLKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            24318..25196
                     /locus_tag="IW746_000490"
     CDS             24318..25196
                     /locus_tag="IW746_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1077418.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            25198..25515
                     /locus_tag="IW746_000491"
     CDS             25198..25515
                     /locus_tag="IW746_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077419.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            25564..26265
                     /locus_tag="IW746_000492"
     CDS             25564..26265
                     /locus_tag="IW746_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1077420.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            26280..27170
                     /locus_tag="IW746_000493"
     CDS             26280..27170
                     /locus_tag="IW746_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1077421.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            27193..27408
                     /locus_tag="IW746_000494"
     CDS             27193..27408
                     /locus_tag="IW746_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077422.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            27420..27761
                     /locus_tag="IW746_000495"
     CDS             27420..27761
                     /locus_tag="IW746_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077423.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            27761..28132
                     /locus_tag="IW746_000496"
     CDS             27761..28132
                     /locus_tag="IW746_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077424.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            28125..28520
                     /locus_tag="IW746_000497"
     CDS             28125..28520
                     /locus_tag="IW746_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1077425.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            28522..28899
                     /locus_tag="IW746_000498"
     CDS             28522..28899
                     /locus_tag="IW746_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077426.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            28900..29508
                     /locus_tag="IW746_000499"
     CDS             28900..29508
                     /locus_tag="IW746_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1077427.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            29508..29849
                     /locus_tag="IW746_000500"
     CDS             29508..29849
                     /locus_tag="IW746_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077428.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            30091..32880
                     /locus_tag="IW746_000501"
     CDS             30091..32880
                     /locus_tag="IW746_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1077429.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNGGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVASSMESFLKVIAANIPRAVSFLSNLFDAVQKFGSALKFMMPFLVPA
                     TTAFGALMFQLKGIPTIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIVGAVVGAI
                     AVFGYFMATNEEVRNKVISVWNDVKDSVLGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            32870..33610
                     /locus_tag="IW746_000502"
     CDS             32870..33610
                     /locus_tag="IW746_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1077430.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            33607..36318
                     /locus_tag="IW746_000503"
     CDS             33607..36318
                     /locus_tag="IW746_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1077431.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            36331..>36560
                     /locus_tag="IW746_000504"
     CDS             36331..>36560
                     /locus_tag="IW746_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1077432.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT        12653 a         5655 c         7572 g        10680 t
ORIGIN      
        1 tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta
       61 cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact
      121 gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga
      181 ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc
      241 aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga
      301 tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat
      361 cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa
      421 tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat
      481 tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt
      541 gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt
      601 caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta
      661 tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt actttgatga
      721 gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac
      781 tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact
      841 tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac
      901 tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg
      961 aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa
     1021 ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata
     1081 cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg
     1141 acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat
     1201 cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat
     1261 tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat
     1321 acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg
     1381 ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga
     1441 gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa
     1501 tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta
     1561 gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt
     1621 tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat
     1681 cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc
     1741 ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc
     1801 cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat
     1861 caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt
     1921 gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat
     1981 gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat
     2041 gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct
     2101 cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg
     2161 atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact
     2221 caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat
     2281 caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat
     2341 gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat
     2401 cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg
     2461 gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca
     2521 atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta
     2581 gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa
     2641 tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa
     2701 gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag
     2761 tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc
     2821 cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct
     2881 tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga
     2941 gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta
     3001 actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc
     3061 tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat
     3121 gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt
     3181 ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag
     3241 ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca
     3301 gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca
     3361 gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa
     3421 atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag
     3481 ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt
     3541 gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta
     3601 gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat
     3661 gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa
     3721 gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa
     3781 taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa
     3841 catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac
     3901 ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt
     3961 ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt
     4021 tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg
     4081 ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt
     4141 ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag
     4201 agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat
     4261 ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc accttatatc
     4321 gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc
     4381 cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct
     4441 ggtattactg aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa
     4501 gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac caatgtagct
     4561 gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat
     4621 acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta
     4681 acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat
     4741 gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg
     4801 tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg
     4861 cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac
     4921 tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct
     4981 cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc
     5041 cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat
     5101 caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga
     5161 ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc
     5221 gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa
     5281 ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt
     5341 tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa
     5401 gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta
     5461 cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt
     5521 aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga
     5581 atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg
     5641 tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga
     5701 agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt
     5761 attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca
     5821 acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa
     5881 gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga
     5941 gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac
     6001 agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc
     6061 ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat
     6121 caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc
     6181 tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt
     6241 gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc
     6301 tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa
     6361 agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt
     6421 tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga
     6481 acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt
     6541 catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg
     6601 ttattgtgca gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt
     6661 acacataaat tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt
     6721 atcgataatt gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc
     6781 atcgaattct tttgatagaa taaattcgac attttttata tctattttag ctggctctac
     6841 ttcaatttca tttaattctt tcattaaagc ttctcgatct tttttgtatt cctctaagtc
     6901 tataacttca ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt
     6961 actatttttc tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc
     7021 aaatttttgt ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg
     7081 cttatttgtg caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc
     7141 gtatatagat tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac
     7201 tgatccctga cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt
     7261 tgtttttaaa tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat
     7321 aggaggacag taatttttat tatttcttaa ttcaccaatg tattttttgt tcgtcaacat
     7381 atttttaaca ctttgataat ctctaacgat tccatactga gagcctaaat aataaacagt
     7441 ttttcttaaa ctaccactag aaagaaagta gttgaatata tctatcacga taggagcatc
     7501 atcatttatt actaatttct tattttctat agagtagcct aacggagttt tcccgcttac
     7561 tacttcacct tgtgcaattt tatttgccat aacagctttg atgcgttctg aatcaatttg
     7621 ggcttccaat tcagcaaaac tcattacttg tgcaatgaaa gttctgccat acgctgtagt
     7681 tgtatcataa tattgttgag aaacagcatt ccaagaaaca ttgtgttttt ctaaaatttc
     7741 ttgagtgttt aaataatgtc gtagatttct aaaccatcta tctaattttg taaataaaat
     7801 tagatctatt ttattttctt ttacatcttg taatagtttt tgaaattcat cacgatttat
     7861 tttttgacca gaaataccac cgtctacata ttcattaact actttcaaat ccttgtgttg
     7921 ttcgatatat tcatagaggg tttctttttg ttctctaaga ctatctccat gtttggcttg
     7981 ttgttcagtt gatactctca tatataatgc tactcgtttc atgttatctc ctttcgaatg
     8041 tatgttctta tcgttgtaaa agaaaagccc gaaggctaat ctttttacta cacatctgtt
     8101 aattctaatg atcttctgta ttgttcagct tgagctagtt tagtaaaaga tacagttccg
     8161 ttataatttt ctaaaacaac tttttcaatt tcatcaagag atactttaaa gaattctttt
     8221 cgttgattaa ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg attttctaat
     8281 gttggtgcat cttctgaaaa tatcattgca tgaacatcaa attcaaaagg tacagaagcg
     8341 ctactaagtt ccttaattct atccataggt tctaaccgtc tagtcattcc tattttatag
     8401 acatcttcac cgaaagatcc gatattactg atgacataaa catagccagc tctagtatta
     8461 agttctcttt gcaatacatc ttctttgaca gtttctagtt ctcttaattt ctcttccagt
     8521 tctttaattt tatcagcata gagttctttt tcaacatcag cactagcttt ttgcatgtat
     8581 gccattaact tagttatctc tgagttaaac tgtcgttctt ctttatcaat gcgtttcttt
     8641 tcacgttcta tttcacgtct taccttttct tcttcaatca tttgttcttt tattgctttt
     8701 tgctgttctt tttcatcgtt aactttaact atgtatgaat agttgagttc taattcttga
     8761 agttttaatt caagtaattc ttttttgaat tctgcttcat cgtttttaaa aagcttgttt
     8821 attttatcaa aagttcttat aattttttct ctagttgaat ctacgttaga aatagtaagt
     8881 ttattgagaa gtccagttat ttctgattct aatggtataa gcattttctt tttaatatta
     8941 ttagcttctc tattattcaa ataagaagga atattaattg caccgtctaa aataaaatcc
     9001 ttttctttaa tctttaataa agaaagtttg ttttttattt cggatgaaga agtttcatct
     9061 atattcggaa taattactga tttatagata gtgaccagag atgtgtcata tttttgatta
     9121 atttcatcgt ctaaccgctg gctttcagca ttaagaatat caattttgtt ttctagcgtt
     9181 gacaagtttt tattcaattt acttatgttt ttctcttttt cagatataaa cgtttcccta
     9241 gcatttaatt cttgagaata ttcctttacc ttactctcta aggcagaagt tttatctttt
     9301 aattttttag cttcagataa tttgctacta cccataatta ttaaaactat tccaactaaa
     9361 aacaatagta ctcccattca aaacgcctcg cttcttttat aaaataattg tcaaaatcat
     9421 ataaaccctt accgaagttg acggctccgg catgggaatt tttttaaaat acaacttttc
     9481 cgataattct aatctgttct ggcgatacaa ttctatcttc gtataagtcg tttatagaac
     9541 gtaaaatgat tgtttgattt tcgtaatctt tatatatctc tttacatgta actccgtcac
     9601 cacctaattc cacaatagca atttccccat cttctacgtc cggttgttct ttaacgaata
     9661 cgaattgtcc attcctgata acaggttcca ttgaatcgcc tataataggt actgcgaagt
     9721 cagcagcact tggaactctc tcaaattctt cctcttcgac aaccgtatca ccgtaagtaa
     9781 gttctgttgg attggcagcc gaagccgaat tgtgaacagt tgggattttt tcttttttag
     9841 gaaaatctag gacattttct tgtttttgtt cttctaatcg attacttgca aattcatagg
     9901 tagcaacttt attttcgtcg ttaagctgat tatatatagg aaggatatta tatatctgat
     9961 tagatataga taaatcaata ttcatcaaat catctatatc aacattgaat atcttagcta
    10021 tctcatttaa ggtttttaat tttggtgtat attttccttt ttcccattcg cttattgagg
    10081 atgaactttt tcttcctagt ttttcggcta gttcttgttg atcaatatta tacttttctc
    10141 taagaaactt tagattttca gcgaacatag taacacctcc ttgagttaat tgtaacaccg
    10201 tttcggaaaa aatgtaacag gtttcttaga aaatatttta gtgtttttaa agaagtattt
    10261 acgcaaaata gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc ggaaaaaccg
    10321 aagtatataa tttagtcaga aagttaaaga gaggaggcga ataaaatatg gataccgcga
    10381 agaaagaaaa atatacattg agacaattaa gagctttaaa gggttattca aaagaagaac
    10441 tgtcaaggaa aagcaaagtg acatcaagaa caatatttat ttacgaaaac gatgtagata
    10501 aaatgaggaa cggaaagtac gccactctag aaaaaattgc taaggcattg ggtgttagag
    10561 tttccgatat ttttttagac cctgattcgg aaaaaccgaa atcaataaca ggtgaaacaa
    10621 aacaagtcag ctaggaggtg tgaatagtgg aagagaagaa tataaaagta ccgactcttg
    10681 aagaatatac aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca atcgcagtta
    10741 ttacggcgaa tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc cctagatatg
    10801 attaatgttc tttatcacgt ggaggtatag gatcgtttcc aaaactatct tttgattgta
    10861 tttgtccatt ctttttttgg acaataaact ctgattgctg ttttttagaa atctttttac
    10921 catactcaac agcatctgat ttattatcaa atagttttgt atcttcggaa ttaccttcac
    10981 cacgtacagc ccatttgcca tttctaggag atacccattg atttttaccc ataacaattc
    11041 acctcgcttt caagttaatt ctattaagag gtgaaaagaa aacaaactga tggaggtgag
    11101 attgtgagta acaagccaaa aatcactttg aatggcacac ctaatcttca aagattagca
    11161 gatgaaatgg ctagaatacg ttctagacat tggggagtta ctgtggtggc aaccataatt
    11221 gaacaaaaag aaaagcccac acagccggca agcaagtagt gggcaccata gaacaataat
    11281 attatatcac acactcgcca cttccgaaaa gaggagttgc tgctctcctt tcaagtttgc
    11341 caaagtccac gtttcttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga
    11401 cgaaggaggg atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac
    11461 acaagattaa ttattgaaac agaagaaggg gaaaaagtag cagaagttac attgaccgat
    11521 gcaactcctg ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt
    11581 gggtgtggat cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata
    11641 aatagctcag atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt
    11701 gtattagtat gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg
    11761 gaattaggta caacatgttg attttttccc atagttttca cctccatttc tatagaaata
    11821 ttttatcaaa tagaaatgga gaaattcaat aaacgaaagg agggatcaaa tgacattaca
    11881 agagcatcaa cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa tgaaacctag
    11941 attgctaggg gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa aaaagtgact
    12001 cagccgtcca aagcaatgag tcacaaacca aaaattatac ctaaggagat tttatcacat
    12061 gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt
    12121 ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac
    12181 aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt
    12241 gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga
    12301 agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt
    12361 gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc
    12421 attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta
    12481 ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa
    12541 cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga
    12601 agcgccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag
    12661 aatggctcga agaccgcaaa agaggtattg gtggctcaga tgttgcaacc gtacttggat
    12721 taaacaaata caaatctcct tatcaattgt ggcttgagaa aacgggtcaa attgaattga
    12781 aagatttaga aagcgaacca gcttattggg gtaatgtttt agaagaagtg gttgctaaag
    12841 agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat
    12901 tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg
    12961 aatgtaaaac agccaatcaa tttctcggaa aagagtggga aggtgaagag gtaccgctta
    13021 gctatctctg tcaagttcag cattacatga acgttttaaa caaagactat tgttatatcg
    13081 ctgttttgat cggtggacaa aaattcattt ggaagcggat tgaacgagat caagagctga
    13141 tcgatacaat cactgaacaa ttagtagagt tttgggaaac gaacgttctt ggaggtatcg
    13201 agcctgtaat tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa tatgcagatg
    13261 tagaagaaaa tcaaacagct ctaccatcac gttttgatga acttatcgag caaaaaagag
    13321 aactcaagcg gacgaaaaaa gaaattgaat catctattcg tcaagtagac aacgagatca
    13381 tcagtgagct aggtaaacgt gaagccagta tcggtatcac acaaagaaac attatcagct
    13441 ggaaacttgt tcgtacaaga cggatgaaca cgaagaaact agcagagaaa tatccagatg
    13501 tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaaaggaga
    13561 tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa gccacagaaa
    13621 caagttgcac caggacagtt agggcttaaa gctctaatga atacaccaac aatgagaaag
    13681 aaatttgaag aagtacttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt
    13741 gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta
    13801 actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca
    13861 ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc
    13921 tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc
    13981 gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat
    14041 ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat
    14101 ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac
    14161 aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg
    14221 gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg
    14281 caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt
    14341 gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa
    14401 gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa
    14461 tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc
    14521 aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta
    14581 tggagcttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa
    14641 gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg
    14701 aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa
    14761 caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca
    14821 ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca
    14881 agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat
    14941 aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac
    15001 attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat
    15061 ctgcaaagtt gggcagataa cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac
    15121 caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta
    15181 tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagcaaa tcgtgattat
    15241 aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt
    15301 tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag
    15361 tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga
    15421 agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat
    15481 tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag
    15541 caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat
    15601 tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct
    15661 ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa
    15721 caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg
    15781 ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca
    15841 acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg
    15901 atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag
    15961 cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta
    16021 tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa
    16081 gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca
    16141 taaaaccagt gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg
    16201 aggtatcaag tttgattcta tcgcggaagc agagtattac gatctagcct tgtggcaagc
    16261 tgaagcgaat ggttggaaag tgaagctcca ggaaaggttt gagctgatgc cgaagtttga
    16321 actagagggc aagaagtatc gcaagatcga gtatattccc gacttcacat tttacaaaag
    16381 cggcaagctg gtcaaagtcg tagatgttaa ggggatgcag acaaaagact ttaagatcaa
    16441 ggcaaagttg ttctgtcatc aatatcaagt gccgttgata ttagccaaaa aatatcggaa
    16501 tacgttcaag gaagagcgtt tttaacgagg tggtccatca tgacaacaga agaagtgatt
    16561 caaatgcgaa ttcgaagcct tcggcgtgag attgacgatc tggaacgaac aaaggcagtg
    16621 atggtcaatg aaacggctag aaaggcaatc gatttgcaca tagagaattt aagaagggaa
    16681 atccgtagat tggaggaatg agcgtggata aggaagcggc ttggcgaaaa ttaatgttgc
    16741 tgattcaaga tgagaactgg caagaagatg aagcagtggt tgctgaagtt cagcgtctag
    16801 gaaagattgc taacggacgt atacgaaaaa agccagacaa aagaaaacag cgcaaaggga
    16861 aaatcgtcgt tgttttacac gaaggcaaaa ttttgatgca aggaacagct agtgagctgt
    16921 ctgcagaaac tggatatacg cgtgggacta ttcgaacgta cgcttggcga aatcacaccg
    16981 atcgaaaagg gcacgaatat aagtatttgg aggaagaaaa atgaacgaaa acaaattaat
    17041 caaattgggt gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt
    17101 cttcgaaaaa atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa
    17161 agatgaagca ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc
    17221 aattcgcttg caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa
    17281 gacaacgatt gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta
    17341 caaggaattt gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct
    17401 tcaaaaggtc gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga
    17461 ttcctctaaa gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg
    17521 gtttgaagtc gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc
    17581 aatcgatgcg atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag acgcagaaac
    17641 tgcaaaaact caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc
    17701 agaatctaat cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc
    17761 tcaagctcgc aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga
    17821 tgaagcaggc aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag
    17881 taggagcagc atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca
    17941 acaacgatga aatgaaattt taggaggaga aataatggac gaactaatca caaaagtaga
    18001 gcagtgggct aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt
    18061 agctgaggaa ttcggggaaa tagcttcggc gatggcaaga agtaatgatg aactatttaa
    18121 agatagcgta ggagacgtta tcgtcacgct gattatcctt tccatgcaaa aagggacaaa
    18181 cgtacaagag tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag ggaaaatggt
    18241 agatggtgta ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata cgaaatacca
    18301 ctaagtgaag cgggcattca agcaattatc aatggtcggg aggttaacat agaacttcct
    18361 gatggtactg aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta
    18421 attgatcgtt ttaacgtgac tgattctgtg gtagagaacc acttaaaaga atttcgatca
    18481 agtatagacg acactttcag attagggagt tgattgacaa tgaacaacag acatcgcaga
    18541 ataacaaaac taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatat
    18601 ggaatttcag cagaagaaac atataaagtg gcaagtcagt ttgttgctga tgcaagtgat
    18661 gctattcgta agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaatgaga
    18721 tgaaactaaa agacggattt tacgctagta gtcacggcat cggcggttta atgctagata
    18781 tgccgacaaa gaaccctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggttc
    18841 gctgtgaagc agaagagttc atttatccgt ttcgtggata tgtagaacac gtctataatc
    18901 actcagcgat cattcgtatt gaaaacacaa tggaatgcga taagtggtta gcgaaaagca
    18961 aagagaattt agctgtagcg agattggtgg atatggaggt tataaacaat gaagtttaaa
    19021 atctttgaag aggacactcg ttataaatta gaaaaagaat taaacgattt tgcgaaaaac
    19081 aatgagattc agcatatatc tttagcaact tctaagagag gttatgcaaa ttactatgca
    19141 gctgttgtga gctatgtaag tcgagaagtg taactcggca aataaaaaaa gccggatcgc
    19201 tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc ggtttgactt
    19261 gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct
    19321 gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc
    19381 gattattacg gatatgccga aggcaccgaa gcacggcaat aaggcagaag acgcgatcat
    19441 tcaaatgatg gatatagaag cggagagaga cgcgattcta gtggctttga tggctcttag
    19501 tctgattagt cgtcagatac tctactacag cttctgtgtg ccagacagct tctcaaacta
    19561 cagaattagc cgtgaagtgg gttattcaga aagaagtata caacggatga aatcggaagc
    19621 tctaatagag tttgcagaag catataaaca cggaagaata attgcttata aataggggga
    19681 ccatataatg tggaaagatt atgtatcgtt gaaagagttg aaaaaagatc ttgttttcaa
    19741 aagaattgta gaatggtcag agagtgaatt aattttagaa gatggaacca aaatggaagt
    19801 tgtatgtagc gaatcagatt gctgcgcatg ggccgaaggt gaattcaaaa atgtaaagct
    19861 tgatgcagtg attacagata taaagatttt tgataaaggt aaccgtctct ataatggtga
    19921 cggacatacc tcttatgctg aagtcgttgt ttatcataat cgtaatgaga tcgctaaagc
    19981 agaatgcaca gcgaatgatg ggaatggcgg ctactattat agtgtttgtg ctttaaaggt
    20041 caaagacaaa ctttgtatag tgactgacgc ataaaaaaat ggcggttttt tggcggaatg
    20101 atggcggttt ttagccattt atcagtgata ttatggtagt gtcgaaagat tagtgatagg
    20161 tctgagacaa aataataata aaaggaacat cgttttatta ttgtttcaca attaagcttc
    20221 gatagacagc agcggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt
    20281 tcttattatc tatcatccgt tgctgtctat tgttacatac agatcgctta ggcggtcttt
    20341 ttattttggc cgaaaaccta cattttcgat ggccgattat tggaggagga atagcatgtt
    20401 caaactatct gaaatcatta agaagtccga cgttgagaaa ttagagatgt taaaaaagaa
    20461 gttgaagaaa tagcagtctc ttcgtgaggc tgttttattt tgctcacaaa aatagaccac
    20521 tatcgggtaa tagtggtcag gaattaaatg aaaaagatgt tagggttgtt agctaagtat
    20581 aacatcataa cgcttacaaa acaatgcaag aaggataaat tattatgaga acttactggt
    20641 atgtgtcatt aaacaataaa tatccgaagc caatgaaagg acagcatagg cgtgtagtca
    20701 tgtctgttca aatgaaggcg aagtattcga ttgtagaaat gatcagagag gcaacgccag
    20761 tagagattga ttattgcaag ctagtctatt gtgggtgcgg ccgttggaaa gaggatcatg
    20821 tacaaaaaaa tatcagcaag tacatataga gaggcggtga ttaaggatgg aaaaaagcga
    20881 tacgaaagcg aacagataca aacctactgc tgcagagaaa aaactcttgg aagtattgat
    20941 aaatccagat aacttaggca aaccggtgca agagttgtgt tcactggcga aaataggtag
    21001 gaccaaatat tacgacgcca tgagcaaaga tggctttgtt gatctagtta acgaaacaac
    21061 aatggatctg atcaaaggaa aagcttctga tgtccttaat gccacttaca agtatgcatt
    21121 gactgaaaaa ggtcatcagg ataggaaaat gattctgact attgctggaa tctatgcaga
    21181 caaacaagaa ctcaaacatt ctggcggcgt cgatatccga aaacagtatg agaaaatgtc
    21241 cgatgaagaa ttagaagagt tggtgaaacg gtatgaaaaa atcaacgact cttaatcgtg
    21301 ttgagtatgc cgttgttcaa agagagctag ccattcgaaa agctaggaaa agtttttggc
    21361 tattctgcca gcttctttat ccagagtttt acaaagatga tcggttgtat ttgaaagatt
    21421 tatgtcatac gctacaagat ttttacgaag ataaaatcga caaaaaaatt ttggttatta
    21481 acatgccgcc tcgacatggg aagactttta cagcgcggct attcgtttta tggatgtttg
    21541 gacaaaatcc aaagacgaaa attattaccg gatcttataa ccaaatcctg tcatctttat
    21601 ttgcgcaaca aacacgtgat ggaatcatgg ttgaaaacga aaatgttaag gctgaatact
    21661 ttaaagatat ttttccagag gtgcaaatca aatatggaga tgctgcaaag ggtttctggt
    21721 ctttagatgg ttcagaagaa aagaattatt tagcgacttc tcctggtgga acatccactg
    21781 gtattggcgc gaattatgta attgttgatg acatcattaa aaccgcagaa gaagcctata
    21841 acgagcgtgt tttagattct cactgggaat ggtataacaa cacgttggcc caacgaatgg
    21901 aacggccacg taaacagata ttgattatga cacgttggtc aagtaatgat ttagccggaa
    21961 aaatgttgac gagacgaaaa aataatgttc atcagatctg ttataaagca gtacaagaag
    22021 acggctcgat gttgtgcaac gagattatga cccacgatga gtacttagat gtggtacaag
    22081 aaatgaatgt agatattgca gaagccaact atcagcaaga accaattgac caaaaaggca
    22141 ggctatatca gaagtttctt acttatgata cgttgcctga taacattatc aaaatatgga
    22201 actacaccga caccgccgac aagggtgccg attactttgc gtctcctgtt tttgctgaga
    22261 cgtctgatca tgatgcttat attatcgatg tcatgtatac aaaagagccg atggaaacaa
    22321 ctgaaatagc ccacgctaat atgattattc gcaatcgagt taataaggta cggattgaag
    22381 gaaacaatgg cgggcgtgga ttcagaagaa attcagaaag aattacaaaa gagcgaggct
    22441 atcgaggtgc agtatttgaa gactttcatc aatcggataa taaactgtct agaatactgg
    22501 cgaatagtgc ttgggtggaa aatcatgttt actatccaga tgattgggcg acacgttggc
    22561 cggactttta tgaggctatg gttagttatc aacgtgaagg taagaacgct catgatgatg
    22621 ctccggatgc agttacagga attgcagaaa cgattataga agcagatgac ttcgagttta
    22681 ttgcttatta ggaggtgggt ctttgtttta ttctggaatc acattgaaaa aatacaaacg
    22741 tttaagaacg aaatattcaa cgcaaatcga agaaggatta ttcgatccta acgcttttat
    22801 ggaagatatg aaaccctttt tcgatgatcg agaaagaaag tatcttgctt atactagcga
    22861 gaaaaacgag atcgacagtc gtgagaaacc aaaaacaaaa atcattaagg tcaataacaa
    22921 gattcatgcg ggaatgtatt cagtggtcgt tgatcaagca gttaaccatt tcacaggaat
    22981 accaattaaa tgggattatg atgtttcgga acagaaaagg acattcattc aacgaatgag
    23041 agacaaatta ctgggtaatg atatcagcgc cccttctacc ccagaagaat ttgaaacact
    23101 cacgagacaa ttaagtacta tgcggtttgg aatgttagac cccgatactg caacatttca
    23161 aggagcgaca gggcttgctt tcagattact ggagcctgtt gagacaaatg atggttggga
    23221 actaagagca agcaacattg agccgtggaa agctgaaaag tacgaaaacg gcgcagtgta
    23281 cattaaagag aaatacgatc catatcaaaa gaagttttat gaagagatga aggttgtcac
    23341 gccgaaatca atatttactt atgctcgata tactgattac cctggcacgg tacaaggagc
    23401 atttaaaacg ctgggtaatg ttcctaatcc tttaggcgtt atcaatctta cggagtttaa
    23461 aaacaataca aaccgttact gtgactttga agttgcagag gagattagtg atgctattga
    23521 tcgtgtttta tctgatcagc agaacgagat tgaacaattc aagttagctt atatgctgat
    23581 cactggggca gatttgtccg aggataaagc caagaagatg atggagcaac tagggatcat
    23641 caatctgaaa gaccctaatg cgaaagccgg ctacgtgaca aaggatctac taaaggattt
    23701 caacgagtat cattttgatc ttcttaaaaa gcaattctac acggtgtgta aagcaattga
    23761 tttcaatgac gaagtcttta agtcgaatag ttctggggaa gcccgaaagt ggcaaatcat
    23821 tagtttagaa gctaagacaa ataccaaaga acagtacttt cgagaaggat tgaaagagtg
    23881 tgccgaggtt atggctgctt atttggattt tgccgagaaa gttacaattg atccggaaaa
    23941 gatcattttc accttctctc gttcgcttcc aacagattta agctacctag ctgaggcatt
    24001 gcctaagttg gcaccatacg tttctaagag gacgattcaa agtcagatcc cgttcgtcac
    24061 agatgttgat tatgaaaacg aaatgattga cgtcgaaagc agacaatctt acccagacgg
    24121 cgagtatgga ggtggcatcg ttgagtcaaa tagaaacgaa gtactggacc aaacgtcgag
    24181 aactggaaga tttagcaaga ctcaaaatgg aaaatcaaac gctaaagaaa ctaagtagtg
    24241 tctttcctga tgcgttgaaa gaaattcaag cggagttatt atcccaagct gatttacacg
    24301 atattaccta tgctgagatg atggaatttt atagtacaag tgatcagaaa aaatatcgtg
    24361 aatacgttga aaagaactac aaatcactga agatgtatga tgcaaaatac aaagagttca
    24421 tcgaagaata ctttccttct ttcgactacg ccaaagtgaa tcggctgctt caaatgaggt
    24481 cagatatttt tcgaattctt gcagagagtg caatagatgc agatgtgaat aaatattttt
    24541 cggatcggtt agaagatatc ttgcaacgtt cgtatgtatc aaacgcaaac gtatttgccc
    24601 agattttgaa cgtagatttg ccgaattact tatcaaagga agaacttgaa cgttacctga
    24661 actatccttg gtcaggtaag acattctcgc gtcgtttatg gggcaatatt tcatctctag
    24721 aacaaaagct atctaacgcc attacaacta gtgtagcaag tggcgaagga gttctcgtgg
    24781 cattacgaac aatgcgagaa gacccagaaa tatgcgatat gttcaaaagg gaggaatcga
    24841 agtacaacaa ggcgatcgaa aatcttgtga gaacagaata cgctaaattc gctcaagatg
    24901 gtattgagaa atcttatatt gaaactggcg tagacgaata caatgtctta actgcaaaag
    24961 atgaaaaggt atgttcaata tgcggtggca aagcaaagaa caacccatac aagttgagtg
    25021 aagcgatcat tggtgagaac cgtgcgcctt ttcatggccg atgcagatgt acagatgttc
    25081 ctaatatgcc gaaattagga aaagatattg acgaagagta tgagcgttta tttggcgatt
    25141 tattagatga gtttgcacat gattcttttg gaattaattt gaaacggagg aagtagaatg
    25201 aaagattttt ttgaagcagt actaacaatt aatgtaaatg ctgatattgc agaagcctac
    25261 aaaacagcta ttgagtctga gaaccatcct aatggcttga gagaccattg gaatggcaat
    25321 tatgcctacg tggttattgg cgatcaaact gttaattatc aagataatac tccagttgat
    25381 aagaataccg ttaatttaac gattcaatta ttatctcatt cattaccaaa tttaaaagaa
    25441 acagttgatt ggtatgaaaa tatgggatgt attgttgtta gaactgacta caaagaagga
    25501 aagtctagta attaggcttt tttattttgt ccgaaatgac gttaaactag cgcaatactg
    25561 ggcttggttg aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg gcgtgaaaac
    25621 gaatcgtggg gcgaaaggag aatgattatg aaacacaaat cattaatgcc aatgaatttg
    25681 caatactttg ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa atcttttgtg
    25741 gaatccaatg aagaagctca aaaatttatt cagtcacaat ctcaaagtgt tgccgacaaa
    25801 caattggaag cttggaaaca aaataactta gataaaatta aacaggatac catcaaggaa
    25861 tatgaggaat ctaagaaaaa caagtcacct gagcaaattc aactggaaaa actacaagct
    25921 gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt tgttgcagaa
    25981 caaattgctg gattagaact agatggagag ttaaaagagt ctatttctca atttatgcta
    26041 aataatcttg ttagttcgga tacagatttc actaagaatg ctgttgaagg ttttacaagt
    26101 gttttgaatg caattaatga gaaacatgca gatgcattaa aagaactaca aatgaagtct
    26161 gcatttggtg gaactcaaca atcgaataac caggttcagc agaacaatga aacattaaca
    26221 aatccagaag aacaattagg acaaattctt caacaattta actaggagag tgaaaaatta
    26281 tgaaaaaaac atctttaaat aatttagagt atttggatat ttcaccagcg attaatgcta
    26341 tgcaagtacc aaatacacct tttttaagct atttacttgg tgctggaaaa acagagccag
    26401 caaactcgac agaaattaaa tggcgtgaat atgatatcaa caacgatgat tcttctgaaa
    26461 aacttgaggg cggagaatat ccagatgctg aatcaggtcg aacttggttt aacaactata
    26521 ctgaaatttt tagaaaatca acctctgtat ctggtacatt agatgctatt aatgtgaatg
    26581 gagtcggaaa tgaattaact aatcaagtag cactacgtgg tatggaaatg aaaattgatt
    26641 tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa tggttctaaa ggtcgtcgaa
    26701 tgaatgggat tttgaacttg atcaattcag caaataaggc agaaacagcc actgcgggtg
    26761 cagtaacaag aaaagatatc gatactttat ttaaattgat gtatgaaaaa ggttatatgg
    26821 gagaaaaact atgcttgatt tctccagata tgcaggagtt aatgactgat gagttagatg
    26881 gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac ttttggattg caaattggaa
    26941 atatcgtgtc taattacggt acaggtattg ctctactaga accatcattg ccaaaaggaa
    27001 caattgccgc aatcgatact aattatgtga aactacgtcc attacgtgaa tggagagcag
    27061 aagaacttgc aaaaacaacg gattctagac gtatcggtct tgtaggtgaa tactctcttg
    27121 aatacaatgc ttcaaactct ggggcaattt taaatttaaa gtctgaataa aagggagtta
    27181 gtactccctt ttttgatagg aggaattaca gtggtaaaaa aagatgaaac taaaaaagat
    27241 gaagtcgtga aatatagagt aggtaaaact aaaaattttg ttgggtttgt tcatcctaaa
    27301 actcgtagat ttatcacagc agattcaaat aacgaattta tcatttctat agatgataaa
    27361 gaagcaattg caattttgga agatgcaatt gatgttaatg aaatttagga agtgatctga
    27421 tggatgaatc gctaaaaacg gaaatcattg agtctacaaa agaagatttt ccagatttga
    27481 gtgaagaacg cataactaat ttattagaaa taattttgct agaaattgaa tcatacaaca
    27541 cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt gattagtgaa gtgttgtata
    27601 aaataataaa aaatgaatca gaaaaaacag tatcttcagt tagacgtggc gatacaacga
    27661 ttagttatgc ttcaacaaca aatgatgtta gtgagttact tctaggctat ggtgatttga
    27721 tacgaagagt tattggttgt ggaggattgg agttttttta atgaatgaag cagatatttt
    27781 agaaactact tatgaagata gttgtattat tgaaagactg acggacattg aagattctaa
    27841 tacaaatatt actattcaag attacaaaaa agtatatgat aatcctattt cttgcgctct
    27901 ttcacaaggt caaatcgatg gactagcagt catagaagat ggagagatgg taaatgtttc
    27961 aactgacaca tataaattat ttgttcatcc taagattaaa ctaaaaaaag gagatcgaat
    28021 aacaataact caaaaggcca gtggcttaat tttttctcta tttgctacta agccttttta
    28081 ctatcctagt cattgtgaag tgaatttgat aggaagtgag aaaaatgggt aatctcaaat
    28141 atgaatcaaa tgcagaaaag atcattgaga attttaaaaa tatgactgta attgctcaaa
    28201 aagaaggaat atccttcgtt aacgattcaa tgaataaagt cgttagttta gctaaaccat
    28261 taactcctgt aaaatcaggt aatttaaggc gaggatatcg tgtagtaaaa gctagaaaac
    28321 tatcaagcgg tcgaattgtt ggagcagtta taaacaatga acattatttt aaatatgtag
    28381 aagaaggtca taggactaaa aatggtggct ttgtcaaagg gaaattcatg ttaactcgtg
    28441 caacgaatct tgcaaatatg tcttatattc ctcgaagatt taaacaaatg gcaataaaaa
    28501 tcgttaagaa aggaaagtaa catgaaagat gaaatcattg ctgcaatcag tagcaaatta
    28561 aaagaaatct atcctgatgg gacaatatat cttgattcag ttatgcagtc aactaaagat
    28621 ttttatttcg ttttatcagt aatggaatct ggaactgaaa atgtaggaat tgatattcaa
    28681 aatgtttctt tcttaattga tattgcattg attgataata aacctaatag aaatttaatt
    28741 aatgaattag tctcacgttg tgggactttt tttaatacga ttacaatcga tgagcaaata
    28801 ttatttccaa aagcctattt acctgatgaa gcagatggtg ttcaacatat tcgttttaca
    28861 ttagaatttc cacaatatat tgaatggagt gaaagataaa tgggagaaaa aagaagtaaa
    28921 accggaatta tttctgtcga aaaacctact tggtttcctt tggaagatga gacagggaat
    28981 ttcccggtat atggtactgc gttgccaatg ggaacagcag taagtatcaa accaacagct
    29041 aattatgaaa caacacaaga ttatggggat tcagtggttc aagatcaatt tacagctttt
    29101 ggtggtgcag aagttgaact ggaagcaaat ggatatactc ataaagtttt atctacaatt
    29161 actggaggga aaattgttaa aggtggtgcc ttgcgttctg gagaagatat tgcgcaagat
    29221 ggtgcgtttg catatagacg taaaaaatcg aatgggaaat accgttatac ggtcttttat
    29281 aaaggacagt ttgctctaga ttcagatgaa acgtcaacga ttgaaggaag taaagtaagt
    29341 tttactcatc caacttggaa gggttcattt gtagatgtac caggacttgg atatatgtat
    29401 tcagttgatg aagatgatga aggcgtggat aaagcaatga tcggaaattg gtttacaaaa
    29461 gtagcaattc caattgaaga agcagaactt tcaggaggta cagaataatg tcaaaatatc
    29521 aaacaacgat taaattaacg aaaaaagatg aagaaggtaa atatgagcaa gtacaattta
    29581 aatctgctga atttttacca ggaacagttg tagaagatgc tgcaggagtt atggaagaaa
    29641 tgcaaacagc gactgataaa caatcagtta aaaaagcttt aagtcgtgct tattcattta
    29701 ttgcagatac tttatttgag ggacaattta ctggtgaaga ttattgcaaa ggaattgatg
    29761 ctcgtgagat tgcctcattg acaggaaaat tattgaagtc tgttactgca ggttttgatg
    29821 aaacttatac agagacgaaa aaaaagtaag tgaggcgctc aagtcacctt catttaagta
    29881 ttcgattact taccgagaat tagatataaa gacgcaatta ctcgaagcag gttggacgtt
    29941 accagaaatt gaacgtactg acttggatga gttaatgcgt ctttttgctt ttagagatgc
    30001 ggtaaaagag catgaagatg ttgagtacta tgataatttc actcaatttt aggaggtgat
    30061 actttgaata acgatgacct aattctgaag atgatactag atgaatctgg ttttactgcc
    30121 ggtatgaata atgctgtcaa aaagttgaac tcttttgatg aaacaatcga aagaaaaagt
    30181 agaaatggtg gcagttcatt aggtagtatt tggaaaatat ttgctggaag ttttctagct
    30241 agcggagtaa ctagaatcgt aggagctggc tttgatctaa tcaagggttc cataagtgga
    30301 gcggttgatc gagtagatac gatgaataac gctctacgaa atttccaaaa tatgggattt
    30361 agtaattcag aaattatgaa gaatatcgga aagaatgggc ttttatctca aggtattcaa
    30421 ggacttccta ctgctttgaa tgatgcgata agtcatgttc aacttcttgc ttcttctaca
    30481 ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa atgatggaat tcttggtttc
    30541 ggtggttcaa ctgatcaagt taatgaagct gttattcaat tatctcaaag tttctcaaac
    30601 ggaaaagtag acgcacaaac ttggaactca atgatcaacg ctcaacttgg acctactctt
    30661 tctgctattg ctaaaaagat gggaattaca atgggagatc tgaaagaagg tttatctcaa
    30721 ggtaagattt ctgttgaaga attccaaaat caattaatag aaatggatac caaaggtggc
    30781 ggaggactta aatcattaag tcaaatcgct aaagattcta ctaagggaat taaaacctct
    30841 atacaaaatg caaaaacagc tgttacacgt ggtgttggtg aggtaataga aggattaaat
    30901 aaagcattag tagattcaga tttaggtgga tttaagggga ttattgataa agttgccagt
    30961 tccatggaat cttttttgaa agtaattgct gcaaatattc ctagagcagt gtctttttta
    31021 agtaatcttt ttgatgcagt tcaaaaattt ggctctgcat tgaaattcat gatgccattt
    31081 ctggttcctg caactactgc ttttggcgca ttaatgtttc aacttaaagg aataccaaca
    31141 attataaaaa gcttcaataa ttttaagaat gccataatcg gtgttggaaa ttcactaaag
    31201 attatggggg ctatagccgc cgcaaatccg tttgttttga ttgttggagc cgttgttgga
    31261 gcgattgctg tatttggcta ttttatggca accaacgaag aggttagaaa caaagttata
    31321 tccgtttgga atgatgtaaa agattccgta cttggtgtat taaagaatat aaaagattgg
    31381 ggaattgata cttggaattc tgctaaagaa atggcatcaa atgctgttga gagtgtcaag
    31441 gatgcttggt ctggaataaa ggaatggttt tcaaatactt ggcaaggcat taaaaatgga
    31501 gcaacaggtt tatttgataa aacagtagaa acatctatga atgcggttga tagtgtaaaa
    31561 aacgcatggt caaatacaaa gcaatggttt tctgatattt ggcagagcat aaaagattca
    31621 gcaactgaaa aatggaatga aattaaaggt tctatcatgg aagttgctgg tccattgatt
    31681 acaggtttaa agaacgcatt cttgcatgtc actttttatt tagagacctt atggaataat
    31741 ctagttgaga ttggtaaaaa tgtttttgag attttaaaaa atgttatact tacgccagtt
    31801 ttgtttatta cctccatgat ttctggtgga tgggaagaaa caaaaaacaa catgattggt
    31861 gtatggaata atattaaaga aagtgctcta aatatttggg agtctataaa aaatattttt
    31921 gttagttaca ttacaaatat ttattttgct gcgcttaata tttggacagg gttcaaactt
    31981 actttgataa acatttggaa tgaagtagta agtcaagcta aatcaatttg gattaatgta
    32041 aaatactttt ttattaatct ttggattgac attaagtatt ttgctattca aaaatggatt
    32101 gaattaaaat tcgggattat tcaaacttgg attgatttaa aatataatgc tattactact
    32161 tggaacaata ttaaacagtt cttcaaagat acttggcaga acattaaaga tatagcatac
    32221 aatacatgga tttctataaa gaattccatg atcaatactt ggaataacat caaggaatct
    32281 ttctggaata ttgttactgg aattgttaac tccgctgaaa atgcatggac taatctaaaa
    32341 aatggtgttt caaaagcaat taatcgggtg aaagaaatct ttgattcatt aagggaaatc
    32401 aatttattcg aaatcggtaa gaatatcatt gatggactta tcaatggtgt agtagaaaaa
    32461 tggaatgcat tgaaaaagac tattaaagga atagctggaa gtatcaaaga ttctattaag
    32521 ggtgcattag gtattcattc tccatctaga tggatgaggg atatggtagg taaaaatatt
    32581 gttcaaggta ttatcgttgg tattgataaa gaaaaaagta aattggatca gacaatgact
    32641 gatttagtga aaactccgtc ggttcaacca gttattacag ggtctaatag tcaaccagtg
    32701 gtacaagcta aacaaaatac atcttcaaat gcagtgaatg aaattcactt gcatttaaat
    32761 gtttatggag atctacctga ttcgatgatt aaacaaatcg ccaagaagat gaaaacagaa
    32821 ttgacgagac aaatgaaacg agatgctgat gcagtaggag ggacattata tgcaacttaa
    32881 aagaggacaa ttttttatca atcaacatta ttcttctgag tttaacgtat atattcaaaa
    32941 taggcctgcc tctgtttcag ctagtcgtgt aattgaattg agagaacgtg aaggcaatga
    33001 ctcaattatt atcgataaag cctattacaa aaacgttact agaaaaattg aatgttatta
    33061 caaagcacca tcgattgatt tagtgcagga atgggaagat cgaattactg aatggttaga
    33121 tatgaactct tacagtgatt ttattcttta ttacgatcaa caatatatct atcaagctat
    33181 agtaaccgaa gcgccagaat ttaaaggaac aagaaaaaca gggaacatag ttccatttga
    33241 atttacagtt agtcttcgac cattcaaaga aaattatagt ggtcgttttg ctattcaaca
    33301 gataaaagct ttcgagttat ttaatccaga gaagtatgct tcaaaaccgc ttattaaatt
    33361 gagtggttct ggagatgctt ctttttatat taataatgat aaatatgatt tgaaactatt
    33421 agatagagaa ttatatatag attctaaact agaagagtct tatcgaaagc tagatgacaa
    33481 tttagaacat caagatcatg tcactttatt tttagatttt ccatttctat accctggaaa
    33541 aaatgaaatc aaatggacga ataacataca ttcgtttgag ataataccaa ggtggtggag
    33601 aaaagtatga taccaagaat atatagtcct accgagacag atttttctac gaatggttta
    33661 gggattttga aggacaccac aaaatgtgag atatatgaag tagcaaatgg aaaatatgaa
    33721 ttagagttgg agtatccatt aggtactcga tttgatgaat attttgaaaa tgactatcaa
    33781 ataaaagcaa agtcaaatga tcaagaagag tatcatatct tttttattga tgataaagat
    33841 attgatacct ttttagatac cgtaactatt tatgcccaga gtcgtacaaa tcggcttgga
    33901 agacgagcgg tcacttttgc tggagtcgac tctaaaactg gtcgagaagc aatggcaatt
    33961 attgaaaaca atatggataa aaaatcagat atacgcttat attctgatat tacaactgta
    34021 tcaagtacaa cttttgaagc aagaaatgtt ttaaattgta ttgctggtga acaaggatct
    34081 ttacttcagt attggggcgg agaaattaaa cgtgagcctt ttaagctatc tttattaaag
    34141 cgaagaggtc gtgataatat tggcacaatc cgatatggaa aagatttatc tgggttaaaa
    34201 gttaagttag attggacagg cgtgaaaaca agaattattc cgtatgctga tcctcaaagt
    34261 gatgcaggta caactagccg aatttatggc tcgccagtag atagcgaata tattaataat
    34321 tatcctgatg tatatacaga acacgttcaa ttcacagaag aacagggagt taaagacgtt
    34381 aatagcttaa ataaaatagc taagaattat ttcaaaacaa ttaatccagg ctgtgataaa
    34441 ccgaagattt ctattaccgt tgagttcgat aaattaacgg acaccgaaga aggaaaagaa
    34501 ttcgctaaga taagaaatta tgggttattt gatacgttta aaatatatca tcgaaaatat
    34561 aaactatatt ttgaatcgaa agttagcggt gttcaatatg attcattatc tgaaaaggtt
    34621 ttgaaattgg aagctgggga tgctcaagtt gctttttatc aacaacaagc tgttactatt
    34681 caagataaat taaaagatta tgctacgaat aattatatga gtagttttaa tgattatgtg
    34741 tcctctatga ttgcaggtca aggaaatgca ggcggttatg tagttttatg gccaaaagaa
    34801 aaaccttcaa atatttttat aatggatagc cctgatttaa acaaggcaaa agaagtatta
    34861 agaatgaaca aaaatggaat tgccttttct aaaaatggtt ggaatggtcc ttttaattcc
    34921 gcttggacat tggatagtat attcaatgct aattttattc aaacaggatt aataaaggca
    34981 gatatctttc aaaactcttt taataaaact ggagatgtat tgaaattagt taatggacta
    35041 cttcaaattt ggaataacaa aaagaaaata atggagttaa ccaaaaaagg aatggagttt
    35101 tggaattcta atagttcaat tggaacgatt ggaacaactg attctgctgg taatcctttt
    35161 cctggagctt ctactcccac acctattgaa gataattctt tagttattcg tacaaatgga
    35221 gacggcaaat atattttgat ttctcctaaa gttggtaaag gattagtttt attaggaaat
    35281 ggtaaagcaa tttattttgg agacttagat gtacaaggca aactcacagt taacggaaaa
    35341 gaaatcacag ggaacaacag tggaggaagt gatcctggaa ctatccctcc tcaattgacg
    35401 acggaagctg aaaaaagagc atggaaaatt tggacaatgc tgaaagctcg tggctattct
    35461 gaatatgcag ctgcaggtat tctaggaaat attcaggggg aggttggagc aagtatgaac
    35521 cctgatacag aacaacttgg tggtccagct tatgggattg ttcaatggga tggttctgct
    35581 tatccgttag tcggatcacc aacttggaac ggacgagagt atgttcagcg tttgatgaac
    35641 accgcaggga ttcaagaaga ttatcgaagt attgaagccc aagtaaaatt attagattgg
    35701 tgtatgttca acggtcaatg gctcggaaaa gtaaatccaa ccacagtatc aggatttaaa
    35761 tcgatcaatg atgccaaaag tgcagcgtat gcttttgaaa tgaacttcga acgcccagct
    35821 tctgcacatc cagaacgcca aaattatgcc caatcttggt ataacaaatt acatggatta
    35881 actagtccag aacctggagg gaatttcatt tgtccaattc aaaaaccagt gacagttact
    35941 tcagaatgtg gatggagaac tagtccaata aatggcggcc aagaatttca taatggaatt
    36001 gatcttgtaa atggaaatcc taatacacct gtttttgcag cattagatgg ggaagttgtt
    36061 caggctggtg ctaattatta tgactggtat ggtaattacg tggttattaa acataataat
    36121 gggaagtgga caggttatgc tcatttgtct cgtattgatg tttctgttgg acaaaaagtc
    36181 cagaaaggtg ctcaaatagg cttgatggga acaactggtc catctacagg agaacatcta
    36241 cattttcaaa ttatgaaaaa ttattggcca cagccagttg ttgattttga gaatccaaga
    36301 aattatatcc aattttaagg tggtgattct atgagtaaat ggaatgtcgt tttaagtaca
    36361 acagaaccat ataattatgt ggggatgatt caagttcgac aaggcaataa gaatacagag
    36421 gttatggaag cgactatagt tgaaaatggt cttccctacg atttatcaga atgtaaggta
    36481 tattttgaat cagttgtagg tgggaaatat ccagtccaat tagaaacaaa aattgtggat
    36541 gctaaaaaag ggaaaattaa
//
LOCUS       DACEND010000011        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEND010000011 DACEND010000000
VERSION     DACEND010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.012"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW746_000505"
     CDS             <1..1152
                     /locus_tag="IW746_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077433.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW746_000506"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW746_000506"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1077434.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW746_000507"
     CDS             2728..3003
                     /locus_tag="IW746_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1077435.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW746_000508"
     CDS             3009..3776
                     /locus_tag="IW746_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1077436.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRIYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW746_000509"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW746_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1077437.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW746_000510"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW746_000510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1077438.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW746_000511"
     CDS             7920..8222
                     /locus_tag="IW746_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1077439.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW746_000512"
     CDS             8225..9388
                     /locus_tag="IW746_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1077440.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW746_000513"
     CDS             9414..12842
                     /locus_tag="IW746_000513"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1077441.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW746_000514"
     CDS             12863..13987
                     /locus_tag="IW746_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077442.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW746_000515"
     CDS             13977..14414
                     /locus_tag="IW746_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1077443.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW746_000516"
     CDS             14398..14736
                     /locus_tag="IW746_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1077444.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW746_000517"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW746_000517"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1077445.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW746_000518"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW746_000518"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1077446.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW746_000519"
     CDS             15893..16936
                     /locus_tag="IW746_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1077447.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW746_000520"
     CDS             17095..17823
                     /locus_tag="IW746_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1077448.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW746_000521"
     CDS             17939..18184
                     /locus_tag="IW746_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1077449.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW746_000522"
     CDS             18181..18399
                     /locus_tag="IW746_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1077450.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW746_000523"
     CDS             18535..19074
                     /locus_tag="IW746_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1077451.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW746_000524"
     CDS             19067..21340
                     /locus_tag="IW746_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1077452.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW746_000525"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW746_000525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1077453.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW746_000526"
     CDS             complement(22513..22767)
                     /locus_tag="IW746_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1077454.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW746_000527"
     CDS             complement(23167..23436)
                     /locus_tag="IW746_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077455.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW746_000528"
     CDS             complement(23906..24361)
                     /locus_tag="IW746_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1077456.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW746_000529"
     CDS             complement(24499..26427)
                     /locus_tag="IW746_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1077457.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW746_000530"
     CDS             complement(26624..27457)
                     /locus_tag="IW746_000530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1077458.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW746_000531"
     CDS             complement(27649..28353)
                     /locus_tag="IW746_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1077459.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW746_000532"
     CDS             complement(28376..29002)
                     /locus_tag="IW746_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1077460.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW746_000533"
     CDS             29328..31703
                     /locus_tag="IW746_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1077461.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW746_000534"
     CDS             complement(31727..32218)
                     /locus_tag="IW746_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1077462.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW746_000535"
     CDS             32405..33325
                     /locus_tag="IW746_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1077463.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW746_000536"
     CDS             33479..33823
                     /locus_tag="IW746_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1077464.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW746_000537"
     CDS             complement(34013..34513)
                     /locus_tag="IW746_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077465.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW746_000538"
     tRNA            35041..35124
                     /locus_tag="IW746_000538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11777 a         6169 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tatacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtaga
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEND010000012        34419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEND010000012 DACEND010000000
VERSION     DACEND010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.013"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(73..1965)
                     /locus_tag="IW746_000539"
     CDS             complement(73..1965)
                     /locus_tag="IW746_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077466.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1962..3689)
                     /locus_tag="IW746_000540"
     CDS             complement(1962..3689)
                     /locus_tag="IW746_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077467.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3705..4355)
                     /locus_tag="IW746_000541"
     CDS             complement(3705..4355)
                     /locus_tag="IW746_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1077468.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4559..4855
                     /locus_tag="IW746_000542"
     CDS             4559..4855
                     /locus_tag="IW746_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1077469.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5052..5666
                     /locus_tag="IW746_000543"
     CDS             5052..5666
                     /locus_tag="IW746_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1077470.1"
                     /translation="MCFSIKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLELYR
                     DDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANALWN
                     LNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQYVK
                     GILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5758..6924)
                     /locus_tag="IW746_000544"
     CDS             complement(5758..6924)
                     /locus_tag="IW746_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1077471.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6936..7289)
                     /locus_tag="IW746_000545"
     CDS             complement(6936..7289)
                     /locus_tag="IW746_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1077472.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7417..8439
                     /locus_tag="IW746_000546"
     CDS             7417..8439
                     /locus_tag="IW746_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1077473.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8878..9312)
                     /locus_tag="IW746_000547"
     CDS             complement(8878..9312)
                     /locus_tag="IW746_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077474.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9422..11281)
                     /locus_tag="IW746_000548"
     CDS             complement(9422..11281)
                     /locus_tag="IW746_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1077475.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11399..12558)
                     /locus_tag="IW746_000549"
                     /pseudo
     CDS             complement(11399..12558)
                     /locus_tag="IW746_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(12865..14289)
                     /gene="araA"
                     /locus_tag="IW746_000550"
     CDS             complement(12865..14289)
                     /gene="araA"
                     /locus_tag="IW746_000550"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1077476.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14305..15003)
                     /locus_tag="IW746_000551"
     CDS             complement(14305..15003)
                     /locus_tag="IW746_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1077477.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14960..16621)
                     /locus_tag="IW746_000552"
     CDS             complement(14960..16621)
                     /locus_tag="IW746_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1077478.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16839..18239
                     /locus_tag="IW746_000553"
     CDS             16839..18239
                     /locus_tag="IW746_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1077479.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18491..19570)
                     /locus_tag="IW746_000554"
     CDS             complement(18491..19570)
                     /locus_tag="IW746_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1077480.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19656..20615)
                     /locus_tag="IW746_000555"
     CDS             complement(19656..20615)
                     /locus_tag="IW746_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1077481.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20627..22150)
                     /locus_tag="IW746_000556"
     CDS             complement(20627..22150)
                     /locus_tag="IW746_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1077482.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22160..22723)
                     /locus_tag="IW746_000557"
     CDS             complement(22160..22723)
                     /locus_tag="IW746_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1077483.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22729..23592)
                     /locus_tag="IW746_000558"
     CDS             complement(22729..23592)
                     /locus_tag="IW746_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077484.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23606..24502)
                     /locus_tag="IW746_000559"
     CDS             complement(23606..24502)
                     /locus_tag="IW746_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077485.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24677..25948)
                     /locus_tag="IW746_000560"
     CDS             complement(24677..25948)
                     /locus_tag="IW746_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077486.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25938..27455)
                     /locus_tag="IW746_000561"
     CDS             complement(25938..27455)
                     /locus_tag="IW746_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1077487.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27653..28543
                     /locus_tag="IW746_000562"
     CDS             27653..28543
                     /locus_tag="IW746_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1077488.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28616..29440)
                     /gene="nadE"
                     /locus_tag="IW746_000563"
     CDS             complement(28616..29440)
                     /gene="nadE"
                     /locus_tag="IW746_000563"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1077489.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29452..30924)
                     /locus_tag="IW746_000564"
     CDS             complement(29452..30924)
                     /locus_tag="IW746_000564"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1077490.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31561..31923)
                     /locus_tag="IW746_000565"
     CDS             complement(31561..31923)
                     /locus_tag="IW746_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1077491.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32146..32898
                     /locus_tag="IW746_000566"
     CDS             32146..32898
                     /locus_tag="IW746_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1077492.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33038..33961)
                     /locus_tag="IW746_000567"
     CDS             complement(33038..33961)
                     /locus_tag="IW746_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1077493.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33976..34248)
                     /locus_tag="IW746_000568"
     CDS             complement(33976..34248)
                     /locus_tag="IW746_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077494.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10156 a         7169 c         5918 g        11176 t
ORIGIN      
        1 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
       61 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      121 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      181 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      241 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      301 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      361 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      421 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      481 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      541 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      601 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      661 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      721 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
      781 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
      841 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
      901 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
      961 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1021 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1081 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1141 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1201 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1261 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1321 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1381 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1441 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1501 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1561 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1621 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1681 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1741 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     1801 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     1861 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     1921 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     1981 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2041 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2101 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2161 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2221 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2281 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2341 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2401 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2461 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2521 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2581 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2641 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2701 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     2761 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     2821 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     2881 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     2941 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3001 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3061 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3121 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3181 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3241 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3301 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3361 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3421 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3481 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3541 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3601 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3661 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3721 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     3781 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     3841 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     3901 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     3961 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4021 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4081 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4141 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4201 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4261 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4321 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4381 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4441 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4501 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4561 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4621 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4681 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4741 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     4801 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     4861 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     4921 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     4981 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5041 aagcatatgg catctgtttt agtatcaaag gtcatcccct aactgcagaa gaatcgcgca
     5101 cagttaaggc attgacgtct tttttaacaa gttataaaga aaatcatcca gatgatgaag
     5161 tgactgtttt ggaactttat cgcgatgata ttccggaaat cgatgaagaa ttattatcag
     5221 gttgggaagc attacgtgct ggtgcagaat tcacttcctt atcagaaagt caacaacaaa
     5281 aaattgctcg tttcaatgaa ttgactgatc agttccttgc tgctgataaa atcgttatcg
     5341 caaacgcctt atggaatcta aatatcccaa caaaattaaa agcttggttc gataccgtca
     5401 atgtagctgg aaaaacgttc cgctatacag aaaacgggcc agaaggatta gtgacaggta
     5461 aaaaagcact gcacatccaa tctaatggcg gtgtatacaa cggacaagac tttgcctctc
     5521 aatatgtaaa aggtatttta aactttgtag gcatcgatca agtagatcag ctattcatcg
     5581 aaggaatcga ttatgaccct gaccgtgcag atgagttgat gcaaaatgca ttagataaag
     5641 ctgctgcttt aggtaaatca ttttaaaaat tgagataaat gcatacaaaa gagacagagg
     5701 aaaaaatctt caaagcagca tttgtgcctt ggatattttc ccatctgtct ctttttgtta
     5761 ttctttctgc tttttcaacg gattttcttc ataaagagaa gagattgttg tgtaccattc
     5821 aaaaacatga gtaatcacta ctttagctgc tgcataaaca ggaatcccca agatcacacc
     5881 gatgattccg aacaatttcc cagaagtcag cagaactaat aaaatcgtta ctggatggat
     5941 tgctaactga ttccctagta ctaatggaga gatcaagcgt ccttctattg tctgctctac
     6001 tgcaaaaacg atcagtactt tgatgatcat gaatggacct gctacaatgg ctaaaaagat
     6061 agccggaatc atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa aacccgcaat
     6121 aatccccaac gtgacagcat actctaaacc aatgaccgca aaaccaatca taaacatgat
     6181 tgccacagca aaagccacag ttagttgtcc tcttatataa gaagaaactt gactattcat
     6241 ttcactcagc acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata
     6301 aggtgctaat tgtttaccgt ctttcaatag ataaaataag ataaacggca tcgtcagcag
     6361 agccactaaa atagtcgcta ccgcgccgac aaaacttcca agactctgaa ctgtactctt
     6421 agatatatct tggatcatat cgcctgccga attcatgaac ttatcacctg ctgtttccaa
     6481 ctgttcgcga aattggttga acagcgggtc ccgcaaaatc tcggttaatt tattatcaat
     6541 cgtatctaca tattgaggga agttactgat gaagctcact gtttgttcct gaatcttagg
     6601 gatgatcact acgctccccc atgcgattag tgcaacaacc aaaacaaaca gcccaatgat
     6661 actgtaaagc cttggtactt ttcgcttttc aaagtaatca actaccggat tcatcaagta
     6721 atacaagatt cctgctaaaa tgataggcag acctacgatt gctaaaaatt gccagattgg
     6781 tgcaaacaaa tatgaaactt ttgtaaataa aaagacgatc agcaatatta acaatacaac
     6841 aagtaaggct gtgactactt gattatttaa aaaccagcgc caaaaccacg ataccggtct
     6901 gttcttctta ttttttcctt ccataattcc tcctgttagt tataaaaaat ttggcttccc
     6961 aaaccaatag caggcagtct atgcggatga agatagattc catttgcaat ttgtccttct
     7021 tctggacaag catcaaaaat caatgatacg tgagctactg tctttaaatt cccattggcc
     7081 atcggaccga cataatcgat cgtatatacc tgatcatcaa aagcaattgt tccgcctgct
     7141 ttcaaagaaa aaggttcgtc ttttgatact tcttgaatca ccgaatattt tcgtaaggca
     7201 gctgttgctg ttttatcgaa caaaatcagc atgggttctt ccttgtttat tgcttctgtc
     7261 ccaatttctg taatctttgc ctgaatcatt gcactctctc ctttaattgc tttcaaaaac
     7321 agtataacac attctcattc tttagccgtg tggaataatt atgtgataga ataaaaacga
     7381 aaactatttc attacaatta ggaggaatca gctcatatga tcgaaaagat aattttagga
     7441 acctatacgc gtcgcgaaag taaaggaatc tatacgatcg accttgatac agaaaaagaa
     7501 gaattagcca acctttcatt agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt
     7561 aaagctggca atctttacac agttactagt gtggatggat taggtggtgc cggtgcgtac
     7621 gataaggatt ttcatttttt gaatgcggta acagagtctg gcgctccttt gtgttatgta
     7681 gcggttgatg aagcacgtca attactatac ggggcgaact atcataaagg tgaagtaaat
     7741 gtctaccata tattaggaga tggcgaactg aaagcagcag attcaatatt ccatcaagaa
     7801 gcgacaggtc ctcataaaaa ccaagatcat gcgcatgtcc actatacgga tctgactcct
     7861 gaccaacgat tagtcgtatg tgatcttggt acagatcgtg tgtatactta tgatgtgtcc
     7921 gaaaacggca aattgaatct tgtaactacc ttcactgcag agccaggaac aggccctcgc
     7981 catcttgtct ttcatccaaa tggacaattc gcttatttat ttggtgaatt agacagcaca
     8041 gtccgcgtgc tttcttataa taaagaaaca ggtgcttttg aagaattaca gaaaatatcg
     8101 accttgccag aagaattcga cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat
     8161 ggcaagttcc tttatgcttc taaccgagga cataactcga ttgctgtatt cgcagttttt
     8221 gaagatggtt cccacattca aaacaagcaa atcatctcaa cagaagggga ttttccccgc
     8281 gatttcgcac tagatccaag caataatttt gtcgtgtgtg ccaatcagaa caccgataat
     8341 ctgacattat atcgccgtca tcctgaaaca ggattactgg aattactgaa aaaagatgtc
     8401 tatgtaccag aatgtgtctg tgtttatttt gatcgttagt aagaaaagga gaatgaaatg
     8461 aatcagcaag acgtattaga cagactacgg gaagaactaa atattccttt ctttgatggg
     8521 aaaatcgaag ataaagagta tagcgaagag gaatatcaga aactcaaagc cgatttacag
     8581 aactactttg aacaatatgt acagaatgta gaaaactaat cagctttcca tactatctga
     8641 aatgaacatc aggacaagac ttttccgtct atcctgatgt tcttttctac aaataaaaaa
     8701 ccgccgagat ggttagactc gacggaaaag gagtatttac tcgattttga gtaagtacaa
     8761 ggttgttgtt ggttataggt atgtcattat cataaaggat tttgatcact ctatcataaa
     8821 ggtaatatta catttatatt agataactct agtttttatt gttatcaaag atttctttta
     8881 ttttttcaac aacaaattag tcattttttg gatggcagct gtcaattggt ctgtcgcttg
     8941 gtcttgtttt gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat
     9001 actttggatc atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt
     9061 atgaatgccc attgcttttg ctggtggacg caaagtactg actttcataa gcattgcccg
     9121 atattttttt gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag
     9181 ttcacttgtt tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc
     9241 ttctccgatt gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat
     9301 attcggtaac atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt
     9361 ttttatcctc aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt
     9421 cttattcgtg ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat
     9481 attggatcaa ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt
     9541 tataccactg tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta
     9601 atgataaaag cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg
     9661 ctgcaaaata aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt
     9721 tactgtcgaa cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga
     9781 tcgtatctga atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag
     9841 aaagattctt caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt
     9901 gcagataatt ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta
     9961 gattatcata ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact
    10021 gaggtgtttt ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa
    10081 tatatggatt attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt
    10141 caaatcctaa tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga
    10201 tcgctgtcgt ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca
    10261 tttttggtac aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa
    10321 aaccagtcaa tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca
    10381 tattgccact atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata
    10441 cttctacgcc gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt
    10501 tcggcgactc ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag
    10561 tgatttcttt gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca
    10621 gattgtacaa aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag
    10681 gtatccatag tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat
    10741 tactgatata ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga
    10801 atcgccttcc ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc
    10861 ctgcggcagc taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga
    10921 tttcagtgat catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca
    10981 tataatttgc aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga
    11041 cagatcctgc tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga
    11101 agaatttttc cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat
    11161 tttgacacca ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg
    11221 ctgctgttcc ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca
    11281 aatgcttgct ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac
    11341 gaaattcgaa agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt
    11401 atggcctttc tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct
    11461 gttgtttcac ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga
    11521 catgctcatt cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca
    11581 gagaaaatat ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg
    11641 gcgcttggaa aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca
    11701 aaagataatc ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag
    11761 tcgatcccct agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg
    11821 aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt
    11881 atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat
    11941 attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta
    12001 atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc
    12061 gcggatagaa taacccaaga catccatccc taagctatag caacttagcg ctacttttgt
    12121 gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata
    12181 agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat
    12241 acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt
    12301 tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt
    12361 ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt
    12421 aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc
    12481 tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat
    12541 caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa
    12601 ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag
    12661 gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca
    12721 aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag
    12781 actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact
    12841 aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact caactaattt
    12901 tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc
    12961 acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac
    13021 acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat
    13081 cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt
    13141 cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac
    13201 gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat
    13261 tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc
    13321 tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc
    13381 aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc
    13441 atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg
    13501 aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata
    13561 atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc
    13621 gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca
    13681 accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt
    13741 gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg caacatccat
    13801 ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt
    13861 ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt
    13921 taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc
    13981 taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga
    14041 gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat
    14101 gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa
    14161 ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag
    14221 tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat
    14281 attcaacata attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg
    14341 ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag
    14401 ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc
    14461 actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat
    14521 tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata
    14581 ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata
    14641 aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat
    14701 cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa
    14761 aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc
    14821 caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt
    14881 gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac
    14941 gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag
    15001 cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc
    15061 cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga
    15121 tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc
    15181 catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg
    15241 ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt
    15301 ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt
    15361 tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga
    15421 aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta
    15481 ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat
    15541 cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt atctccatac
    15601 ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt
    15661 tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg
    15721 gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg
    15781 aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc
    15841 cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag
    15901 cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt
    15961 gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt
    16021 cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc
    16081 ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat
    16141 taatttcttt cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc
    16201 atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga
    16261 ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat
    16321 aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga
    16381 tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt
    16441 ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg
    16501 caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa
    16561 tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca
    16621 taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt
    16681 acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat
    16741 aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat
    16801 caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt
    16861 cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg
    16921 gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt
    16981 ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat
    17041 ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt
    17101 ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg
    17161 cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata
    17221 tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga
    17281 ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa
    17341 cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg
    17401 gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg
    17461 caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa
    17521 tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag
    17581 aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag
    17641 gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag
    17701 cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc
    17761 tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg
    17821 tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa
    17881 attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg
    17941 ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat
    18001 ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg
    18061 tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt
    18121 taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa
    18181 tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa
    18241 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc
    18301 acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca
    18361 gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga
    18421 tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa
    18481 aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat
    18541 agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat
    18601 cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc
    18661 ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt
    18721 ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta
    18781 aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg
    18841 tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga
    18901 taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac
    18961 gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta
    19021 aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc
    19081 cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag
    19141 gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac tctttctcca
    19201 attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat
    19261 tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac
    19321 cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg
    19381 tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc
    19441 gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg
    19501 tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat
    19561 attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca
    19621 aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca
    19681 ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta
    19741 ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata
    19801 tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt
    19861 ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga
    19921 ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca
    19981 gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa
    20041 aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac
    20101 gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct
    20161 tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg
    20221 aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct
    20281 atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta
    20341 tgattgcttg cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc
    20401 cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca
    20461 tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc
    20521 gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc
    20581 cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac attttcttta
    20641 ttctaattac attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta
    20701 ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca
    20761 acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt
    20821 caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc
    20881 cgttatctac gatcacactg tccacataag gaacattttt tatcttttca caatcataaa
    20941 cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca
    21001 tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta
    21061 ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag
    21121 ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg
    21181 gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat
    21241 caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag
    21301 caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat
    21361 catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt
    21421 tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt
    21481 cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat
    21541 ccactaactt catgactttt gaggcttcat ttgctaacct accatattca taagcactct
    21601 tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa
    21661 aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat
    21721 tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga
    21781 atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat
    21841 ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta
    21901 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
    21961 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
    22021 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
    22081 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
    22141 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
    22201 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
    22261 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg
    22321 cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc
    22381 aggataaaag aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga
    22441 ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa
    22501 ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc
    22561 ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga
    22621 tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa
    22681 ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc actctttcat
    22741 tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa
    22801 tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc
    22861 tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa
    22921 tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt
    22981 aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat
    23041 gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat
    23101 accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag
    23161 caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag
    23221 caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc
    23281 cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt
    23341 tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata
    23401 gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga
    23461 aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt
    23521 tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct
    23581 ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca
    23641 cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg
    23701 catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt
    23761 ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg
    23821 aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa
    23881 tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg
    23941 gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa
    24001 cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg
    24061 gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag
    24121 aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac
    24181 aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa
    24241 tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc
    24301 gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga
    24361 aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata
    24421 ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc
    24481 aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa
    24541 tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac
    24601 acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc
    24661 tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca
    24721 tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt
    24781 tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt
    24841 tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca
    24901 agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat
    24961 atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat
    25021 atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag
    25081 tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt
    25141 caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg
    25201 gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag
    25261 gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca
    25321 tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac
    25381 tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt
    25441 cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta
    25501 cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt
    25561 taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc
    25621 cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt
    25681 tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt
    25741 caattttgat gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt
    25801 caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag
    25861 attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag
    25921 agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat
    25981 ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt
    26041 acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta
    26101 tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga
    26161 acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca
    26221 tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt
    26281 gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat
    26341 ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata
    26401 acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg
    26461 attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga
    26521 ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc
    26581 cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg
    26641 cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga
    26701 gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg
    26761 tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa
    26821 cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc
    26881 tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt
    26941 tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt
    27001 aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca
    27061 tctactccac gagtacccag attgacagcc atattgattt cggcccccac atgctgacac
    27121 cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc
    27181 aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca
    27241 aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg
    27301 tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat cccttcatat
    27361 actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca
    27421 cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta atttatacat
    27481 gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt
    27541 tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt aatataactt
    27601 ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact
    27661 acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa
    27721 gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg
    27781 agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa
    27841 aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag
    27901 ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca
    27961 ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg
    28021 gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg
    28081 gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt
    28141 ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg
    28201 gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg
    28261 agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca
    28321 atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc
    28381 cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat
    28441 cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg
    28501 taattacgat caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt
    28561 ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt
    28621 tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc
    28681 atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa
    28741 tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa
    28801 gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt
    28861 gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa
    28921 atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg
    28981 ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt
    29041 tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag
    29101 ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg
    29161 catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt
    29221 catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag
    29281 tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca
    29341 aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt
    29401 tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc
    29461 tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca
    29521 gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc
    29581 ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc
    29641 gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg
    29701 aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg
    29761 agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt
    29821 gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt
    29881 gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc
    29941 aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt
    30001 agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat
    30061 ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc
    30121 catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc
    30181 cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac
    30241 gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg
    30301 gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac
    30361 attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc
    30421 cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt
    30481 gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt
    30541 ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa
    30601 aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata
    30661 ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt
    30721 gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc
    30781 gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc
    30841 taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact
    30901 gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta
    30961 cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac
    31021 atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta
    31081 ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa
    31141 gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc
    31201 gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac aacataatca
    31261 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
    31321 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
    31381 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
    31441 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
    31501 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
    31561 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
    31621 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
    31681 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
    31741 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
    31801 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
    31861 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
    31921 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
    31981 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
    32041 tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc
    32101 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
    32161 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
    32221 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
    32281 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
    32341 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
    32401 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
    32461 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
    32521 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
    32581 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
    32641 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
    32701 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg
    32761 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
    32821 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
    32881 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
    32941 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
    33001 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
    33061 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
    33121 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
    33181 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
    33241 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
    33301 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
    33361 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
    33421 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
    33481 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
    33541 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
    33601 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
    33661 tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
    33721 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
    33781 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
    33841 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
    33901 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
    33961 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
    34021 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
    34081 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
    34141 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
    34201 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
    34261 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
    34321 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat
    34381 tataatgata ctgtaaatat atctttatta ttagtgaat
//
LOCUS       DACEND010000013        31434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEND010000013 DACEND010000000
VERSION     DACEND010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.014"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..490)
                     /locus_tag="IW746_000569"
     CDS             complement(23..490)
                     /locus_tag="IW746_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1077495.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(610..2148)
                     /locus_tag="IW746_000570"
     CDS             complement(610..2148)
                     /locus_tag="IW746_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1077496.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSVMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2228..2914
                     /locus_tag="IW746_000571"
     CDS             2228..2914
                     /locus_tag="IW746_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1077497.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW746_000572"
     CDS             complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW746_000572"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1077498.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW746_000573"
     CDS             complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW746_000573"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1077499.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4400..5530)
                     /locus_tag="IW746_000574"
     CDS             complement(4400..5530)
                     /locus_tag="IW746_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1077500.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5530..7713)
                     /locus_tag="IW746_000575"
     CDS             complement(5530..7713)
                     /locus_tag="IW746_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1077501.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7710..8735)
                     /locus_tag="IW746_000576"
     CDS             complement(7710..8735)
                     /locus_tag="IW746_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1077502.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8780..9607)
                     /locus_tag="IW746_000577"
     CDS             complement(8780..9607)
                     /locus_tag="IW746_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1077503.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9604..10671)
                     /locus_tag="IW746_000578"
     CDS             complement(9604..10671)
                     /locus_tag="IW746_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1077504.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10697..11533)
                     /locus_tag="IW746_000579"
     CDS             complement(10697..11533)
                     /locus_tag="IW746_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077505.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11530..12399)
                     /locus_tag="IW746_000580"
     CDS             complement(11530..12399)
                     /locus_tag="IW746_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077506.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12469..13752)
                     /locus_tag="IW746_000581"
     CDS             complement(12469..13752)
                     /locus_tag="IW746_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077507.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13766..15451)
                     /locus_tag="IW746_000582"
     CDS             complement(13766..15451)
                     /locus_tag="IW746_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1077508.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15726..16751
                     /locus_tag="IW746_000583"
     CDS             15726..16751
                     /locus_tag="IW746_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1077509.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW746_000584"
     CDS             complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW746_000584"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1077510.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW746_000585"
     CDS             complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW746_000585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1077511.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25423..26391
                     /locus_tag="IW746_000586"
     CDS             25423..26391
                     /locus_tag="IW746_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077512.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26409..27314
                     /locus_tag="IW746_000587"
     CDS             26409..27314
                     /locus_tag="IW746_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077513.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27311..28117
                     /locus_tag="IW746_000588"
     CDS             27311..28117
                     /locus_tag="IW746_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1077514.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28274..29242
                     /locus_tag="IW746_000589"
     CDS             28274..29242
                     /locus_tag="IW746_000589"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1077515.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29559..30083)
                     /locus_tag="IW746_000590"
     CDS             complement(29559..30083)
                     /locus_tag="IW746_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1077516.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30370..>31434
                     /gene="scm"
                     /locus_tag="IW746_000591"
     CDS             30370..>31434
                     /gene="scm"
                     /locus_tag="IW746_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1077517.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8822 a         6788 c         5921 g         9903 t
ORIGIN      
        1 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
       61 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
      121 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
      181 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
      241 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
      301 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
      361 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
      421 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
      481 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
      541 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
      601 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
      661 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
      721 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
      781 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
      841 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
      901 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
      961 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     1021 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     1081 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     1141 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
     1201 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
     1261 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
     1321 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
     1381 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
     1441 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
     1501 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
     1561 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
     1621 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
     1681 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
     1741 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
     1801 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
     1861 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcacagagga
     1921 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
     1981 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
     2041 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
     2101 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
     2161 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
     2221 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
     2281 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
     2341 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
     2401 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
     2461 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
     2521 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
     2581 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
     2641 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
     2701 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
     2761 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
     2821 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
     2881 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
     2941 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
     3001 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
     3061 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
     3121 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
     3181 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
     3241 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
     3301 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
     3361 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
     3421 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
     3481 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
     3541 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
     3601 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
     3661 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
     3721 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
     3781 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
     3841 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
     3901 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
     3961 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
     4021 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
     4081 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
     4141 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
     4201 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
     4261 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
     4321 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
     4381 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
     4441 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
     4501 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
     4561 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
     4621 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
     4681 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
     4741 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
     4801 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
     4861 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
     4921 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
     4981 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
     5041 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
     5101 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
     5161 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
     5221 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
     5281 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
     5341 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
     5401 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
     5461 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
     5521 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
     5581 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
     5641 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
     5701 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
     5761 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
     5821 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
     5881 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
     5941 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
     6001 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
     6061 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
     6121 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
     6181 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
     6241 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
     6301 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
     6361 atgatcatcg acttttacag ctctgctcag ccaaaaagcc gctgtttcat gaatgagcgt
     6421 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
     6481 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
     6541 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
     6601 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
     6661 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
     6721 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
     6781 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
     6841 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
     6901 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
     6961 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
     7021 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
     7081 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
     7141 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
     7201 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
     7261 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
     7321 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
     7381 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
     7441 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
     7501 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
     7561 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
     7621 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
     7681 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
     7741 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
     7801 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
     7861 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
     7921 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
     7981 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
     8041 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
     8101 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
     8161 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
     8221 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
     8281 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
     8341 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
     8401 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
     8461 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
     8521 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
     8581 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
     8641 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
     8701 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
     8761 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
     8821 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
     8881 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
     8941 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
     9001 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
     9061 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
     9121 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
     9181 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
     9241 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
     9301 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
     9361 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
     9421 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
     9481 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
     9541 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
     9601 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
     9661 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
     9721 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
     9781 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
     9841 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
     9901 ggcaacataa gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc
     9961 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    10021 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc
    10081 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    10141 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    10201 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    10261 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    10321 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    10381 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    10441 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    10501 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    10561 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    10621 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    10681 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    10741 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    10801 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    10861 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    10921 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    10981 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    11041 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    11101 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    11161 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    11221 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    11281 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    11341 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    11401 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    11461 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    11521 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    11581 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    11641 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    11701 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    11761 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    11821 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    11881 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    11941 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    12001 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    12061 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    12121 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    12181 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    12241 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    12301 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    12361 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    12421 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    12481 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    12541 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    12601 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    12661 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    12721 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    12781 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    12841 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    12901 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    12961 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    13021 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    13081 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    13141 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    13201 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    13261 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    13321 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    13381 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    13441 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    13501 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    13561 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    13621 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    13681 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    13741 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    13801 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    13861 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    13921 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    13981 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    14041 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    14101 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    14161 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    14221 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    14281 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    14341 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    14401 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    14461 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    14521 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    14581 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    14641 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    14701 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    14761 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    14821 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    14881 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    14941 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    15001 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    15061 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    15121 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    15181 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    15241 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    15301 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    15361 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    15421 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    15481 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    15541 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    15601 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    15661 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    15721 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    15781 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    15841 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    15901 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    15961 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    16021 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    16081 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    16141 aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    16201 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    16261 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    16321 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    16381 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    16441 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    16501 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    16561 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    16621 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    16681 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    16741 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    16801 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    16861 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    16921 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    16981 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    17041 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    17101 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    17161 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    17221 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    17281 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    17341 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    17401 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    17461 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    17521 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    17581 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    17641 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    17701 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    17761 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    17821 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    17881 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    17941 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    18001 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    18061 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    18121 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    18181 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    18241 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    18301 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    18361 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    18421 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    18481 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    18541 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    18601 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    18661 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    18721 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    18781 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    18841 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    18901 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    18961 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    19021 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    19081 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    19141 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    19201 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    19261 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    19321 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    19381 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    19441 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    19501 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    19561 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    19621 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    19681 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    19741 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    19801 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    19861 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    19921 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    19981 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    20041 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    20101 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    20161 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    20221 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    20281 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    20341 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    20401 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    20461 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    20521 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    20581 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    20641 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    20701 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    20761 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    20821 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
    20881 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    20941 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    21001 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    21061 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    21121 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    21181 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    21241 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    21301 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    21361 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    21421 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    21481 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    21541 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    21601 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    21661 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    21721 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    21781 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    21841 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    21901 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    21961 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    22021 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    22081 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    22141 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    22201 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    22261 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    22321 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    22381 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    22441 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    22501 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    22561 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    22621 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    22681 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    22741 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    22801 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    22861 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    22921 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    22981 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    23041 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    23101 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    23161 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    23221 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    23281 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    23341 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    23401 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    23461 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    23521 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    23581 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    23641 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    23701 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    23761 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    23821 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    23881 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    23941 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    24001 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    24061 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    24121 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    24181 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    24241 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    24301 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    24361 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    24421 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    24481 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    24541 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    24601 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    24661 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    24721 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    24781 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    24841 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    24901 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    24961 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    25021 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    25081 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    25141 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    25201 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    25261 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    25321 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    25381 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    25441 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    25501 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    25561 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    25621 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    25681 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    25741 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    25801 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    25861 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    25921 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    25981 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    26041 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    26101 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    26161 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    26221 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    26281 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    26341 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    26401 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    26461 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    26521 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    26581 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    26641 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    26701 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    26761 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    26821 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    26881 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    26941 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    27001 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    27061 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    27121 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    27181 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    27241 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    27301 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    27361 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    27421 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    27481 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    27541 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    27601 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    27661 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    27721 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    27781 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    27841 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    27901 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    27961 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    28021 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    28081 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    28141 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    28201 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    28261 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    28321 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    28381 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    28441 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    28501 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    28561 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    28621 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    28681 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    28741 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    28801 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    28861 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    28921 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    28981 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    29041 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    29101 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    29161 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    29221 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    29281 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    29341 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    29401 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    29461 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    29521 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    29581 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    29641 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    29701 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    29761 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    29821 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    29881 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    29941 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    30001 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    30061 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    30121 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    30181 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    30241 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    30301 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    30361 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    30421 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    30481 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    30541 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    30601 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    30661 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    30721 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    30781 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    30841 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    30901 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    30961 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    31021 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    31081 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    31141 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    31201 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    31261 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    31321 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    31381 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACEND010000014        30888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEND010000014 DACEND010000000
VERSION     DACEND010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.015"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(280..3558)
                     /locus_tag="IW746_000592"
     CDS             complement(280..3558)
                     /locus_tag="IW746_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1077518.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3829..4374)
                     /locus_tag="IW746_000593"
     CDS             complement(3829..4374)
                     /locus_tag="IW746_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1077519.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4386..5078)
                     /locus_tag="IW746_000594"
     CDS             complement(4386..5078)
                     /locus_tag="IW746_000594"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1077520.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5097..5381)
                     /locus_tag="IW746_000595"
     CDS             complement(5097..5381)
                     /locus_tag="IW746_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077521.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5394..5957)
                     /locus_tag="IW746_000596"
     CDS             complement(5394..5957)
                     /locus_tag="IW746_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1077522.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6185..6847
                     /locus_tag="IW746_000597"
     CDS             6185..6847
                     /locus_tag="IW746_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1077523.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6971..8167
                     /locus_tag="IW746_000598"
     CDS             6971..8167
                     /locus_tag="IW746_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1077524.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8268..9644)
                     /locus_tag="IW746_000599"
     CDS             complement(8268..9644)
                     /locus_tag="IW746_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1077525.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9681..10547)
                     /locus_tag="IW746_000600"
     CDS             complement(9681..10547)
                     /locus_tag="IW746_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1077526.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10636..11661)
                     /locus_tag="IW746_000601"
     CDS             complement(10636..11661)
                     /locus_tag="IW746_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1077527.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11679..12353)
                     /locus_tag="IW746_000602"
     CDS             complement(11679..12353)
                     /locus_tag="IW746_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1077528.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12537..13430)
                     /locus_tag="IW746_000603"
     CDS             complement(12537..13430)
                     /locus_tag="IW746_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1077529.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13504..15201)
                     /locus_tag="IW746_000604"
     CDS             complement(13504..15201)
                     /locus_tag="IW746_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1077530.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15194..16507)
                     /locus_tag="IW746_000605"
     CDS             complement(15194..16507)
                     /locus_tag="IW746_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1077531.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16507..20025)
                     /locus_tag="IW746_000606"
     CDS             complement(16507..20025)
                     /locus_tag="IW746_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1077532.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20318..20776)
                     /locus_tag="IW746_000607"
     CDS             complement(20318..20776)
                     /locus_tag="IW746_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1077533.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21050..21592)
                     /locus_tag="IW746_000608"
     CDS             complement(21050..21592)
                     /locus_tag="IW746_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1077534.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVICINCTNKA"
     gene            complement(21594..22211)
                     /locus_tag="IW746_000609"
     CDS             complement(21594..22211)
                     /locus_tag="IW746_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1077535.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22208..23062)
                     /locus_tag="IW746_000610"
     CDS             complement(22208..23062)
                     /locus_tag="IW746_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077536.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23059..23970)
                     /locus_tag="IW746_000611"
     CDS             complement(23059..23970)
                     /locus_tag="IW746_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077537.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24011..25345)
                     /locus_tag="IW746_000612"
     CDS             complement(24011..25345)
                     /locus_tag="IW746_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077538.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25553..26383
                     /locus_tag="IW746_000613"
     CDS             25553..26383
                     /locus_tag="IW746_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1077539.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26627..26876)
                     /locus_tag="IW746_000614"
                     /pseudo
     CDS             complement(26627..26876)
                     /locus_tag="IW746_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27067..27261)
                     /locus_tag="IW746_000615"
     CDS             complement(27067..27261)
                     /locus_tag="IW746_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077540.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(27766..27951)
                     /locus_tag="IW746_000616"
     CDS             complement(27766..27951)
                     /locus_tag="IW746_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077541.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(28801..29142)
                     /locus_tag="IW746_000617"
     CDS             complement(28801..29142)
                     /locus_tag="IW746_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1077542.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(29142..29372)
                     /locus_tag="IW746_000618"
     CDS             complement(29142..29372)
                     /locus_tag="IW746_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1077543.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29659..29997
                     /locus_tag="IW746_000619"
     CDS             29659..29997
                     /locus_tag="IW746_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077544.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30202..30441)
                     /locus_tag="IW746_000620"
     CDS             complement(30202..30441)
                     /locus_tag="IW746_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1077545.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(30417..30764)
                     /locus_tag="IW746_000621"
     CDS             complement(30417..30764)
                     /locus_tag="IW746_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1077546.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9063 a         6588 c         5292 g         9945 t
ORIGIN      
        1 cgtcctgacc tgagtattag agaaaaaaac ggaaaaatag ttcctcatat tttgccgttt
       61 gttgagctaa taccgagggt cagctgattg aacaccgttt agtggagaga ttgtgaaaaa
      121 gctgtcctgc atcaagtaat aagaacagcc aatcaaaagc agcaaggaga acctcatcta
      181 taaaaagata gaaggaagtg agacatctat tggtctcatt tccttctatt tttactttcc
      241 tcagtcaatt gttttacatc ataaaaggag aaattccact tatttttttc gctgtttcat
      301 gacataaaca actacaccga ttaacaaaat tcctccagct aatgctagca acgggttatt
      361 aaattctccc gtttttggga gtccttgagt gccatttttt tcactttctt ttgctgtcct
      421 atccggatga gaaggttttc catttggttt tccatttggt gattcatttc ctggattttc
      481 tctttctggt ttgtcatttc ccgggggcac atcggcagtt tctcgcacag tgatcgcttt
      541 ttgctgggta acagattctg tatgagcaga ccatttttcc ccatcaaata tttccgaacg
      601 ataagtgact tctagcgtat attctccttt tgaaggtgct gaaaaaatag cggtatgatt
      661 agaaaattga gcacttgact gtgtttcaac gatttggatc ttttctggga aataacgcat
      721 atcttcaaag acaggcgttc cttgatttgg tgtgaaagtt gacacaaaaa ctgtaaaagg
      781 cttcagttca atgatatttc cttctgcatt ctccgccaca ccatttgctt cttgtataat
      841 aatcggatta ttttgttcat ttggttttgt ctgatttggt tcttgtacga ttgcatactc
      901 tggatattcc gaagacggtg aggtttctcc gtcaaaaata gcacctacag ataatggaag
      961 accatccact ctttcctttt ctgtttgacg attccccggt ttaatctgca tcagtccatg
     1021 aacatggata cttccatttt catagcgcgt aacaggctgc tgaccttctt tcgaacatgt
     1081 atttagagac tcgatccaat cttttgatat ggtatgtcca agagtatttt ctgattgttt
     1141 ctctcgttca ttccaatagt agttcaacga gtggtagatt tgatcttcgt tttgattcaa
     1201 tgggcggatc tgctctctca tctcaagatt ctctgttcgg aaagataaga taccagaagc
     1261 atggtagtct gtgtttcctg ctgaagaggt ataaagagcc acattatatg aaccgttatt
     1321 aaaacttgta ctgctattga cgataatatc tgttccgcta ttggaatcga ttcctttcgc
     1381 aagattttcg aaagacaaac tattgatcaa ttcatgaggg cccgttaaag aagaaccgcc
     1441 cattttgaat ccattaccgt taccttctcc ctcaattcct ggtacccagc cgtttcgata
     1501 agcaactgag tttttgatga ttacttttcc gatcgaacct gtttcatttt tagcaaaaag
     1561 gtcccaaccg tcatctgcat tatgatacgc gatacaaccg tcaaatacat ttccttctcc
     1621 tattgtcagt ttggccgcaa atccatcggc atcttcaaat cctggatcgg cattttcaaa
     1681 agatgtacag ttcttgacta agttatgcgc gggccatgct tcgaaaggat catttgagct
     1741 accgctgatt tggattcctg tatttccatt ccgataagca tgaatctctt cgatcaaatt
     1801 atgatttcca gaaatctgta atcctttttg catagctgca ctatttgtaa tgtcaaaccc
     1861 tcgtaaaccc cagtagtgac tgaacagtgt catcccattt ccagttcctt taaaatcaag
     1921 tacaggacgc ttgttttctg gatttttctc tgcaatcaac tgaatcgggt tttctcttgt
     1981 cccgttaacg ccttttggaa tcgtcaatcc tctttcgaag gaataagttc caggcgccat
     2041 gacgattgtc tgcccagcat atgcgtatct caatgcttgt gtcagagata atggattttg
     2101 tctgcttccg tttcctgcat ctgacccttc tggtgttaca taaactgtcg aatatttagg
     2161 tttacggtag tccactgtat gaagaatctc ttttgtttca taactagtca tctctacgta
     2221 ttcttctggc ggatagtttt tatctggcgt aaaactgaaa gtaaattcgt tcgttccttc
     2281 catcagtttt gtctgataat caaattcccg attcgcagtg atagccaagt tttttgcgtt
     2341 cttaagcacc tctcctgttg ctttattttt aattgtcaat aatccgtcag catttgctct
     2401 aaaggacata gtatgatctt ggtttcctga atgattacta gaggtcacag tgtaaatcgg
     2461 atcaatcaag cgagtaggtc tttcttgccc aggaggatct gtcgcaggat ctgagtaagc
     2521 aacttcgatt ccttctacct tgatcgtcat attccttgct gcagcaaatc ctgcataaat
     2581 aaccgattca tctgaagcgt ataatttttc ccagtcatac atgatgtcag aagccatttc
     2641 ttctcctgtt tcaggatcgt aataatatgc ctctaaacca gtattcgttt ttttcatctc
     2701 tacgtccagt ttagtaatcg agttcgtggc cggaaacaga ttcccatgac cgtttccgaa
     2761 tatattataa gaacctgttt ctttttccaa gaaccctgct gaagtctcta aaggtgttgt
     2821 ttccactgag acactcccag gagctggcgc agcttgcgga tcttcagatg atattcctgt
     2881 gattgcccgt gtccccaatc caagtcgcat cgtatatttg ctgccgctcg tgttgaccac
     2941 ttcttgtcta tccgagtccc agacatattc gatccgactg cctagtatgg caaatgaatt
     3001 tgaataaaat gcctgcccaa acaaatgatt ttttggtact gcgtctctta ccattaaagc
     3061 aaatccttct tgggcattcg tataggtcca agattctatt tcgatcgtcg cccgcatcct
     3121 aaagttgatg tctttgggga ttgcttgata ataataggtc attccatctg atccgcttga
     3181 ctggaatttt ccgccgttat tcgtggaaga gagccgcacg ccgtcttcta atggttcaat
     3241 ggcataccta ctagcacttg tgctttctcc gaaaacaacc cctttccact cttcatttgc
     3301 ttcttcagat tgcttttttg tcttttcatt ttctgattct ttcatcatag gatcatttcc
     3361 ttcttccgta tccatatctt cactagttct tggtatttcc tccattgttg acatcccttc
     3421 aatgttcacg tgtgcatttt cttcgttcac agactgttgt tcgttagcag caacttcaac
     3481 gacagaaatt aaaaccgggg tactgagcat aaggagattc atcattagcg cactgtattt
     3541 tatacgtttt tttaacaagt gtattcctcc cgtaacaaat tagaaagcgt ttacttaaag
     3601 tattacctaa tttattttta ctgtcaataa aaaacaaaat tataataagt gtattttata
     3661 aaaagaaaaa atataactta agttgttgat aataaagaga tttattaacg taaaaagaat
     3721 aagttttttt aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg ttttatgctc
     3781 ttcttttatt attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc
     3841 tgcgcctagt gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact
     3901 tgccacggca tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt
     3961 atgcaacaca cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc
     4021 tcgcaaacgg atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca
     4081 ataaacattt tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc
     4141 ccgtccaccc gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga
     4201 atcttttgga tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt
     4261 ctttgtatga gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt
     4321 tccatcctct gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt
     4381 cctccttata cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg
     4441 tcttcgataa attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca
     4501 cgtacgatca aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc
     4561 atttcgcaag ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag
     4621 tcgacaaatg tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt
     4681 gctgctttaa ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct
     4741 ggtaattgtc ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg
     4801 tctgatataa cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta
     4861 ttgatcacca tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag
     4921 acttttccta taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata
     4981 aataaagcgc ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt
     5041 acttcttctt ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat
     5101 ataaatgcga ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc
     5161 atcaaaaaag agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt
     5221 ggtttattct ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga
     5281 taagctgttt ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc
     5341 ttgcgaagtt cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt
     5401 cctttggtca atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac
     5461 tgatctttca tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg
     5521 tcttcatctt gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga
     5581 aggacttcat ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc
     5641 gcacgatagc cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct
     5701 tctcctggat cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga
     5761 aattgtttga caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca
     5821 ggatgaaata ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca
     5881 gcaacatcaa taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat
     5941 tgctcattgt tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca
     6001 tttttcaatt tttctcaaaa actccttatt agtttaccat agataaacct tagattttct
     6061 ctaaatatag aaaatccttc tttagtccga tactctttat agtagtccgt atgctaaaat
     6121 aagtatgtaa tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt
     6181 tattatgata aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt
     6241 ccgaaacaat agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt
     6301 gtggggactc tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac
     6361 tgacgaactt gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt
     6421 tcgacaaaca atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca
     6481 gacagccaaa cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt
     6541 attcaaagaa ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac
     6601 gcatctgccg aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat
     6661 caaaaacaaa cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc
     6721 agctttaatc gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt
     6781 agagatatct gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa
     6841 ggattaactc ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt
     6901 cgagtacttt ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga
     6961 ggaatttttt atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac
     7021 acttgaagat ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca
     7081 acaagcggag attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact
     7141 accggcggaa cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc
     7201 acagtctgtc atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc
     7261 gatgttgaat catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact
     7321 gatgtatcgt ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt
     7381 ccagcgtgtc ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa
     7441 gattggtgca caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct
     7501 aaaagataat ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact
     7561 gaatatttat atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc
     7621 agaagcgatg aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga
     7681 ttacacgcaa ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca
     7741 gatcacgatc cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc
     7801 tgaaaagatc caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat
     7861 cgcattgacc ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac
     7921 aacaaacgac ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc
     7981 gaaagaaaat gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt
     8041 gatcgaaaca atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca
     8101 tgcagaagta gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac
     8161 ccaatagatc acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact
     8221 gttgatgggc gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc
     8281 atcttgctgt gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata
     8341 ccgaacacga tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg
     8401 gagacgattg gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca
     8461 aacatttttc tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg
     8521 atattcgtta gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc
     8581 acaccagcgg cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata
     8641 aacttaggaa gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc
     8701 atcgagatcg ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact
     8761 gttccatgtc ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta
     8821 tacggaacga ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt
     8881 atcgaaccaa tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc
     8941 attgcaacgg catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca
     9001 ccggaaaaac caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct
     9061 acaatgataa acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt
     9121 aatgctttct tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca
     9181 atcaagtcaa tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt
     9241 tcggccccta aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag
     9301 gaaaccgcta gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca
     9361 ccaaatacaa catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat
     9421 tctgcgaaac ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac
     9481 agtgctggac cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg
     9541 atgatcgcac caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca
     9601 ctcgtatgat tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat
     9661 tttaggttgg aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa
     9721 atgactaaaa ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca
     9781 aggatgaaaa tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca
     9841 acagctaaac caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat
     9901 aatccacaga tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag
     9961 aagcttgcgc ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa
    10021 ttcgcccagt tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct
    10081 ctaaaaccct taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct
    10141 tgtcgggcag tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca
    10201 taatctttcg tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt
    10261 aattgaaaac cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg
    10321 aactgtccat tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc
    10381 caaggggtga ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg
    10441 atcgtcattc ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca
    10501 atactacccc atgccaacat agggactaat gctattaaaa tttccataac caatctcctt
    10561 ttatttcaca tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc
    10621 gtttctaacg attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt
    10681 cattgttgtc ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc
    10741 cagatttttt gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt
    10801 tctcccagta aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc
    10861 tgaatcttta actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt
    10921 tccgtgatcc ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat
    10981 ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt
    11041 ttggataaaa taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata
    11101 ttcttggatg aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc
    11161 tacgtattca tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat
    11221 atcgaactcg atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg
    11281 gacccatgag tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac
    11341 ttcttggtgt tcacggatca gctgattgat atattcttct accagttttt cttcttctgt
    11401 cctcccacca ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc
    11461 ccaccatctt ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc
    11521 aatcaaagct aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat
    11581 cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt
    11641 caaatattta aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt
    11701 cctacttttg tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga
    11761 ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc
    11821 agcaattttt cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga
    11881 tattcatttt tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca
    11941 ttcaacgtaa tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa
    12001 cggcgaagta atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca
    12061 aaatcatctg cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag
    12121 ataaaaatga tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc
    12181 aaaacgggaa gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag
    12241 aacacttgat tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc
    12301 atgatcaatt gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat
    12361 ccctccatct ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct
    12421 tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg
    12481 cgtttgtctc ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat
    12541 ctgttatttt tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa
    12601 agcgttagca ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt
    12661 tttgtctgtt ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact
    12721 agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc
    12781 ttttctctaa ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact
    12841 tcataagcga gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct
    12901 tcaagatccg taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt
    12961 gtactctctc ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac
    13021 aaaagatcat tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg
    13081 gctccgatca tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct
    13141 tgctcaaaag gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc
    13201 tgatttttct tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca
    13261 agctgctttt gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct
    13321 aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt
    13381 aataaaagac tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc
    13441 ttcttattta aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt
    13501 tgcttatact tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa
    13561 acttattccg attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc
    13621 gcgtttcata ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc
    13681 ttcattatag cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag
    13741 tacataacct tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg
    13801 aacctcaaag tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca
    13861 ctcaattcta tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat
    13921 tgcgtattca gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact
    13981 gcttgatgca tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc
    14041 tactccttct tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt
    14101 atggaaatca tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc
    14161 tcctccacgt gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc
    14221 tgtcaaaatc gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt
    14281 tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac
    14341 tgtaggcgta tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga
    14401 atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag
    14461 atcaatataa gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag
    14521 gtcgctttcc tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa
    14581 atgcacatct gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat
    14641 atcaggcatc atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc
    14701 tggcgaataa gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt
    14761 cacttggacg gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact
    14821 tgtcagtcga tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc
    14881 ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc
    14941 gtaatatact tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc
    15001 aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt
    15061 tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag
    15121 ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt
    15181 gacaatcatt tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac
    15241 ctaacaataa aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga
    15301 atacggacca ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt
    15361 ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga
    15421 ctggaacttt cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg
    15481 gttggccttt tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt
    15541 catccatctc gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca
    15601 agaaacgttg tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt
    15661 tgtcattcaa aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag
    15721 gagccatatc taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac
    15781 cttctaatgg ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg
    15841 gatctcttag ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg
    15901 attctgtcgg gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat
    15961 caacatccaa tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc
    16021 gatcttggtt tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg
    16081 tatctttccc agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct
    16141 gacggatatt gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt
    16201 tttcagcagc ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc
    16261 catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag
    16321 ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc
    16381 gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg
    16441 gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct
    16501 ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc
    16561 tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt
    16621 agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt
    16681 ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg
    16741 ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt
    16801 ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct
    16861 atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc
    16921 attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc
    16981 tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat
    17041 actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg
    17101 tagtacacca tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac
    17161 cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga
    17221 tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg
    17281 cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa
    17341 gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc
    17401 tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa
    17461 tgtatggcct tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta
    17521 agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca
    17581 attttcttca cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa
    17641 gatccatgtg tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat
    17701 tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc
    17761 tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac
    17821 tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt
    17881 cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc
    17941 gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt
    18001 gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt
    18061 aggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt
    18121 accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc
    18181 taacatctca tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac
    18241 taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc
    18301 agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt
    18361 tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa
    18421 ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc
    18481 tttatgttcg taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt
    18541 ctcataatcc tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc
    18601 ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa
    18661 agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc
    18721 cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta
    18781 cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc
    18841 tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct
    18901 cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt
    18961 gtaatatcgc ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg
    19021 tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga
    19081 gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat aagcaactgt
    19141 gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga
    19201 accatcacca acccagtgag tcgctttatt atctccttct tttacctctc cataacgata
    19261 gtgtacattt acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt
    19321 aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt
    19381 atcttcagca atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt
    19441 acgataactt gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat
    19501 taggattttt tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc
    19561 atttttaagc cctagtgtaa gcggagttcc tccgattggt gtacgattta atgctgtatt
    19621 ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg
    19681 ctcatggtta ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc
    19741 cagactcggg tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc
    19801 atttttagtt tgactattgg tcccagcggt taattcactc atactcgctg acttatctag
    19861 aacaaaaacg atatcgattg gactgatttc ctttttctcg cccccgatga catctagata
    19921 gacattgtaa agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag
    19981 aacaggttct acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa
    20041 attccgttca tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc
    20101 tgcagttgat ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt
    20161 ataatcacta tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata
    20221 gcgttcattg ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt
    20281 catctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga
    20341 tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga
    20401 cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg
    20461 aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga
    20521 ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt
    20581 caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga
    20641 atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat
    20701 agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca
    20761 attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa
    20821 tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga
    20881 tatccttttt agttaaaaaa caccaattat acaacattta ttattaaaat taaattataa
    20941 aattttataa cgttctcatt taacaaaact taagttatta agacaaatga ataaacaact
    21001 acgctttcct cgaaacaaaa aaataaagca caaatgcgca tttacgcact catgctttat
    21061 tcgtacaatt aatacagata acggcagcgc ataggatgcc aataacactt cctaaaatag
    21121 ctcctcctaa aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa taagcgatca
    21181 aacaagcaaa taaaaaggct aatattcctg caactggact taattcgaac aacgcagcta
    21241 ctgataaaaa ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg
    21301 tgtaaaggac aagccgacga tttttttcat aaggtctttt ccgtcttgtc gtttttttga
    21361 taaaaagcaa accaacaaaa aaatagatca aactgccgat aagaatggct agaccatatt
    21421 ctctagtatc aggtatcaag actaaaacag cagcaaggag caaccaaata aaaccaccat
    21481 tagagatatt actgataaat ttcaaaaaat tgatttttcg actatccgtt ttccgttgct
    21541 tggcgaactt gttgataata ctatcatcga ttttagcaat caaacgcaac atgttattcc
    21601 tcctttttaa gaaccttttc ttgcgtacga tggataaaaa gcaggacttg aaaataaaac
    21661 aagaaactga aacctaataa agcaaaatag attggtcctg caaaccagaa tgcgacaaga
    21721 aggatcaacg tgcctgccaa atagaaaagc gcgcttttga aatcattaaa aaacaagaaa
    21781 aatgcagtaa tccaagaagc ttctttttgt tccagttgaa gcaagatttt gtaactaaag
    21841 agaagcgcgc ttaatagaag caaaaacaac gtagtataca tcaatgtata gccaattccc
    21901 ccaccccaat agcggaaaat ctgataatcg atcaatagaa gaagaaaata aacacttgct
    21961 aaactatctt gctttccata aaaagagaag ttctcacgaa actttttcca gaatagtgaa
    22021 ccgagctttt tctcttcatt ttttttgtaa tgataaatca tatacagaac tgttcgactt
    22081 gcagttccta atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata
    22141 agacgtggaa tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat
    22201 cgattcatca tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg
    22261 caaaaaagaa cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat
    22321 tccattgtgt cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt
    22381 cttgatcttg gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat
    22441 tgatcgcaat ggtaatcaag gcaggtttcg tcaatggaag catcaaatga ctccaaatat
    22501 acaaatgatt cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt
    22561 aaaattgacg catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg
    22621 gcaaataagt acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta
    22681 caaaccccgg gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa
    22741 aatcaagctt cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta
    22801 ctagcacagt gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg
    22861 tatccagata gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga
    22921 tttcttccat tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga
    22981 ttcctattac tgtcatgagg acataactta gtattttttt tgtttgccga ttcacactgg
    23041 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
    23101 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
    23161 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
    23221 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
    23281 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
    23341 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
    23401 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
    23461 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
    23521 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
    23581 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
    23641 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
    23701 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
    23761 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
    23821 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
    23881 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
    23941 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
    24001 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
    24061 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
    24121 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
    24181 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
    24241 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
    24301 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
    24361 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
    24421 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
    24481 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
    24541 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
    24601 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
    24661 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
    24721 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
    24781 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
    24841 ataacttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat
    24901 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
    24961 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
    25021 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
    25081 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
    25141 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
    25201 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
    25261 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
    25321 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg
    25381 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
    25441 aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta
    25501 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
    25561 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
    25621 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
    25681 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
    25741 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
    25801 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
    25861 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
    25921 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
    25981 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
    26041 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
    26101 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
    26161 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
    26221 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
    26281 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
    26341 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
    26401 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
    26461 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
    26521 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
    26581 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
    26641 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
    26701 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
    26761 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
    26821 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
    26881 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
    26941 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
    27001 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
    27061 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
    27121 agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
    27181 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
    27241 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
    27301 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
    27361 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
    27421 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
    27481 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
    27541 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
    27601 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
    27661 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
    27721 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
    27781 aaaataacca acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat
    27841 aaatgttgaa aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata
    27901 ccctataaaa gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca
    27961 cttccttaaa taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga
    28021 ctcaaaataa aaaaaaactt acattatact ggaatgaact gtattgatag tgtacctaaa
    28081 agctctactg acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt
    28141 aatcaattag agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg
    28201 agattctact ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg
    28261 gtgtcgttag aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt
    28321 ctgtatggat gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat
    28381 atgccttttt gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc
    28441 caaattttac gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag
    28501 tttagatttt ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt
    28561 gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa
    28621 aaacgaatga cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag
    28681 tttaagatac ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg
    28741 ccttttttta atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc
    28801 ttaaaacata tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc
    28861 tatttttttg agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg
    28921 tccttttata tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga
    28981 agtaattgga catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac
    29041 tagtcctggt cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac
    29101 aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct
    29161 tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta
    29221 aatggatctt caatttttgg aactaacgta attgttccat catcagaata cacaacgata
    29281 tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa
    29341 cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac
    29401 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
    29461 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
    29521 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
    29581 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
    29641 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
    29701 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
    29761 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
    29821 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
    29881 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
    29941 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
    30001 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
    30061 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
    30121 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
    30181 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
    30241 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
    30301 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
    30361 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
    30421 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca
    30481 caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg
    30541 gcattaatca tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga
    30601 gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct
    30661 ccagctgcta attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc
    30721 tgaaggagct ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat
    30781 ctttttttct atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt
    30841 gtagaactaa aaaagttgtg acaagtgaca aactctccag tgaatggt
//
LOCUS       DACEND010000015        30598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEND010000015 DACEND010000000
VERSION     DACEND010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.016"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            41..823
                     /locus_tag="IW746_000622"
     CDS             41..823
                     /locus_tag="IW746_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077547.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            863..1474
                     /locus_tag="IW746_000623"
     CDS             863..1474
                     /locus_tag="IW746_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1077548.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            2504..3676
                     /locus_tag="IW746_000624"
     CDS             2504..3676
                     /locus_tag="IW746_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1077549.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            4054..4617
                     /locus_tag="IW746_000625"
     CDS             4054..4617
                     /locus_tag="IW746_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077550.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            4604..5488
                     /locus_tag="IW746_000626"
     CDS             4604..5488
                     /locus_tag="IW746_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1077551.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            5882..6379
                     /gene="dfrG"
                     /locus_tag="IW746_000627"
     CDS             5882..6379
                     /gene="dfrG"
                     /locus_tag="IW746_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1077552.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(6451..8403)
                     /locus_tag="IW746_000628"
     CDS             complement(6451..8403)
                     /locus_tag="IW746_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1077553.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(8410..8706)
                     /locus_tag="IW746_000629"
     CDS             complement(8410..8706)
                     /locus_tag="IW746_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077554.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            9272..9526
                     /locus_tag="IW746_000630"
     CDS             9272..9526
                     /locus_tag="IW746_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077555.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            9923..11524
                     /locus_tag="IW746_000631"
     CDS             9923..11524
                     /locus_tag="IW746_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1077556.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            11758..12057
                     /locus_tag="IW746_000632"
     CDS             11758..12057
                     /locus_tag="IW746_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1077557.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            12191..12568
                     /locus_tag="IW746_000633"
     CDS             12191..12568
                     /locus_tag="IW746_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1077558.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            12617..14980
                     /locus_tag="IW746_000634"
     CDS             12617..14980
                     /locus_tag="IW746_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1077559.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            15298..15897
                     /locus_tag="IW746_000635"
     CDS             15298..15897
                     /locus_tag="IW746_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077560.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            16009..16233
                     /locus_tag="IW746_000636"
     CDS             16009..16233
                     /locus_tag="IW746_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077561.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            16585..16821
                     /locus_tag="IW746_000637"
     CDS             16585..16821
                     /locus_tag="IW746_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077562.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            16901..17755
                     /locus_tag="IW746_000638"
     CDS             16901..17755
                     /locus_tag="IW746_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1077563.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            17768..18205
                     /locus_tag="IW746_000639"
     CDS             17768..18205
                     /locus_tag="IW746_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077564.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            18223..18522
                     /locus_tag="IW746_000640"
     CDS             18223..18522
                     /locus_tag="IW746_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077565.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            18525..18968
                     /locus_tag="IW746_000641"
     CDS             18525..18968
                     /locus_tag="IW746_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1077566.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            19113..19436
                     /locus_tag="IW746_000642"
     CDS             19113..19436
                     /locus_tag="IW746_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077567.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            19854..22664
                     /locus_tag="IW746_000643"
                     /pseudo
     CDS             19854..22664
                     /locus_tag="IW746_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22760..23515)
                     /locus_tag="IW746_000644"
     CDS             complement(22760..23515)
                     /locus_tag="IW746_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1077568.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(23530..24756)
                     /locus_tag="IW746_000645"
     CDS             complement(23530..24756)
                     /locus_tag="IW746_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1077569.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            24864..25691
                     /locus_tag="IW746_000646"
                     /pseudo
     CDS             24864..25691
                     /locus_tag="IW746_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25751..26113
                     /locus_tag="IW746_000647"
     CDS             25751..26113
                     /locus_tag="IW746_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077570.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            26347..26769
                     /locus_tag="IW746_000648"
     CDS             26347..26769
                     /locus_tag="IW746_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077571.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            26781..28196
                     /gene="cpaF"
                     /locus_tag="IW746_000649"
     CDS             26781..28196
                     /gene="cpaF"
                     /locus_tag="IW746_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1077572.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            28213..29028
                     /locus_tag="IW746_000650"
     CDS             28213..29028
                     /locus_tag="IW746_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077573.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            29028..29825
                     /locus_tag="IW746_000651"
     CDS             29028..29825
                     /locus_tag="IW746_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077574.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            29843..30205
                     /locus_tag="IW746_000652"
     CDS             29843..30205
                     /locus_tag="IW746_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ1077575.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            30377..30598
                     /locus_tag="IW746_000653"
     CDS             30377..30598
                     /locus_tag="IW746_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077576.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT        11332 a         4039 c         5558 g         9669 t
ORIGIN      
        1 gaatttgaag taacccaaat tccataagag aggtgattgt atgaagccaa atccttcaga
       61 agcaggaaaa cgaatcaaac aattacgcct ttcatgtggt tttactatgg aagaacttgg
      121 aaggaaaata gataattcac cacgtgcgac catatccaac tgggaacgtg ggaccaattt
      181 accaaatcca caaaagctga aattattatc aactatcact aattcaacaa ttgactggat
      241 taaatgggga actttagaag aatatataac ttcatacctt atcgatatag ggtatgagct
      301 atatattaag gactttccag aaatacctca caaagtcttt aaagacattc aagaaagata
      361 tagtaatacg ttttcgctaa ataaagacta tgagttattg aatcctataa taaaaaatat
      421 atttacaaaa tattactcaa aagattttga agattatcga gacatagaag ttaaaccaga
      481 ccctaaaaag gtagggagaa aaattcgttc tattagaaaa aaattaggac ttactatgca
      541 ggaatttggg tacgaggttt ctaattctcc tagaagcact gtgtcaacat gggaacatgg
      601 tggtaactta ccgaacaaag ccaaactaaa aaaaattgca gacattgcga attgtagtgt
      661 agaagatttg ctttttgatg aaggctttat ttctgaaaaa tcccaagtta aattgataaa
      721 tgaactcaaa gaagaaaatt caaagctaaa agaaaaaata aaatactatg aatgtttatt
      781 tgatggtata gagagtctgc tgaactcacg aaaaaattcc tagttaatta aactagagta
      841 aaattatttg taggtgttaa atatgttagt tggctatgca cgagtcagtt cagatgatca
      901 aaatttggaa agacagcttg aagaatttag aaaaattggt gtcgaaaaag tatttgctga
      961 gaaaaaatct ggacgtaaca cagtagaacg gccagagttc aataaaatgt tgtcatttgt
     1021 cagagaaggg gatacgcttg tttttgagtc gcttgaacgc ttaggaagaa attctgatga
     1081 aatactagaa acagtatctt ttatagataa aaatggtatg ggcttaatgg ttttgaattt
     1141 accaatttta gatacaaaat ttggtgatcc aaacttagaa aaacttgtta gaagcttagt
     1201 aattaacatt ttttcttgga cggctcaaaa tgagagggag gaaattaaga gaaaacaaaa
     1261 acaaggaata gcaattgcga agaagaaagg agtctataaa ggaaaaccct acgaatattc
     1321 ttctactgca aagaatccac aaaagaggtt tatctataat gaaatagtaa gaatgcttgg
     1381 agaaggtgtt ccgattaagc gaattgcaga aaaaacagga acaaatagaa atttagtcta
     1441 tagaataaag aaaagaataa gtgaatctga ttgattttaa gttctttgag aaatcaatgt
     1501 tacgagagaa ggattctaag cctgtttcaa acttttaagg tgtacaatta tacaaagaag
     1561 cttctaaaca aaagaattaa ttttgtggca gtcaagtaac gacagaaata gaaaagaaag
     1621 aggctatgcg agagtgaatc aacaagttaa ttgggttaat gattttgaaa aagaatttcg
     1681 actgaaattt tttggaaatc ctacagcaga aagtacagaa gaaaaaaatt atgactggtc
     1741 agaattttag gttgcatatt cgattcaata agtgttgaag ataatagttc tcttacgcca
     1801 cagtatacaa cgttagaata tgacgaaaac agattcaata catttccttg caataccatt
     1861 caagaatcaa ttgataagca tgagtgataa ttgattatct acaggaaggt gttttttatg
     1921 aaactagaga tcaaagaagt tgtctgcgat tggggaattt atgtagacgg agaaacatat
     1981 ccatttatga tttttaatag taaagcaaat gcacaagaga taatgcgtat catggagttg
     2041 gataataagc acgaaagatt tgattaagta aaaaaatgga gaatgaatgt gaagcagtgg
     2101 aataccaaaa aacatgcgcc aataataagt atattttagg tgcaatggga ttaggtaaat
     2161 cagcttctat taaacaggtc tacgaagaat cacaggagaa ttgcgtatat gaaaattcga
     2221 attaaagagt gagaatatgt gggagctaat ttatttagtt ttatacttaa taggaaaatc
     2281 aactgaattt gtatataaca ataaggtaat atttatattg ttcttaatag tttgtattat
     2341 cgtatttgat gtctatgatt tactaattca taagaatgaa aatagtaata ttggtagaat
     2401 tagataaagt ccgtataatt gtgtaaaagt aaaaaggcca tataacagtc cttttacggt
     2461 acaatgtttt taacgacaaa aacataccca ggaggacttt tacatgaccc aagtacattt
     2521 tacactgaaa agcgaagaga ttcaaagcat tattgaatat tctgtaaagg atgacgtttc
     2581 taaaaatatt ttaacaacgg tatttaatca actaatggaa aatcaacgaa cagaatatat
     2641 tcaagcaaaa gaatatgaac gaacagaaaa ccgacaaagt caacgaaatg gctattatga
     2701 gcgcagcttt acgacacgtg taggcacgct agaattaaaa gtacccagaa cacgtgatgg
     2761 ccatttttca cccacagtgt ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc
     2821 aatgttggaa atgtatgtat caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga
     2881 actttgtggt aaatccgtct ctaagtcctt cgtttctagc ttaacagaac agctagaacc
     2941 tatggttaac gagtggcaga atcgtttatt atcagaaaaa aattatcctt acttaatgac
     3001 cgatgtactc tatataaaag tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat
     3061 agcgattgga ataaccaaag atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg
     3121 cgaaagcgaa gagacctgga caacattttt tgaataccta aaagaacgcg gtttacaagg
     3181 tacggaactc gttatttctg atgcgcacaa aggattagtc tctgccatta gaaaatcctt
     3241 caccaacgta agttggcaaa gatgccaagt tcacttccta agaaatatct ttaccaccat
     3301 tcctaaaaaa aattcaaaat ctttcagaga agctgttaaa ggaattttta agttcacaga
     3361 tattaactta gcgcgtgagg ctaaaaatcg attgattcat gattatatcg atcaaccaaa
     3421 atattcaaaa gcttgcgcat cattggatga tggattcgaa gacgcctttc aatataccgt
     3481 acaaggaaat tcccacaatc gactaaagag taccaatcta attgaacgac tgaatcaaga
     3541 agtacgcaga agagaaaaga ttattcgcat cttccccaat caaacatcag ccaatcgctt
     3601 aattggagcc gttcttatgg acctacatga tgaatggatt tattcttcaa gaaaatacat
     3661 caattttgat aagtagaaat ggtaaaaaca ttgtatagca ttttacacag gagtctggac
     3721 ttgacttaga attataatca caaacttaat agtaattact attgggatta caacattaac
     3781 aaataaagct ttaatatcaa gtatgatttt aatagtgttt gattttatta tcatttatag
     3841 tttgctgttt ttaataaaga aagtaactga atatttaaat ccgttaggca cgaaataaat
     3901 tttaattgag gttgaattat agcggtaaat catacaaaat agaaatgtag tcttgttaag
     3961 cgtcttgact atctaatttt gaatgaggag ggttgtatat agaaaaaggg tagttaaatt
     4021 cgtagatcaa aaggctacga aggagaataa agaatgacag ctatttataa agcactaaaa
     4081 aattctaacg tatcagagta cgttaagtcg agagtattaa ataataattt agattacatt
     4141 ttagaaaatg gtgttgctga tttaatcagt aaattaagtg atcctataga tatagatcat
     4201 ttggtggtat ggcaagatgg tactttagaa gtatcaatat atggagaatt ttatagtaga
     4261 aataaagtaa ctacattcaa aaatagtatt agtggtcgag aagatttaaa aggaatcaat
     4321 aaaaatagta aattctatag aacaaataaa gaattgctag aaaaaatttc aaagcgtgtt
     4381 tttgaattgg tgaatccatt ttcaaaaaaa cgtagtaaag aactttttgg aattttttat
     4441 gatggtttta gtgcaaaaag taatgtagaa aaattttttt attttaatga ttcagcttat
     4501 attgcggtta aagacagtaa aagaatccct gaagcaactt tagaatattt agagccaatt
     4561 aaattgtctg ttttttattc actttatgag aaagaaatcg ctgatgaagc ggtttaaggg
     4621 gagtaaaatg agtaaaaaaa taggcaaaga gtcattatcg ctcggattga aaaaagaaat
     4681 tgttaaattt aaaagtatgt atagagagcc ggaatttaga ctcatttttc aattagatat
     4741 agtgagaggg ctgaaaatta gttttagttg tttaggttat ctggtctggc tattcattgc
     4801 tagagtagga tggagcaatg cttatagctt ttataaactg ttcgccggaa ctgatcgatt
     4861 aaaagaatca ttagaggtga aagaagcctt aaatatttta ggaagtcttg ctttcagcct
     4921 gtttattttt gctttattta tcttacaagt aggaaaaata atgatgtcaa caaatgacaa
     4981 ttatattaat tatttgaagc cttttcaaaa aggaataaaa atcatttgta gacagggcac
     5041 agttttattt ctacttagcc tgtatgagca tgaaattcat tttcaaaatt attataatac
     5101 gacaactaaa cttggtgcat ttttactcct atatttagtt agtaatatag ctgaaaaaat
     5161 gctgtttaaa catattgtaa agcaagtctt ttctgatatt caaaaggaaa gctattgttt
     5221 aaaaacacct tatggaaatt taaaaaatgt tgagctgttt ttatttaaaa tggatagtaa
     5281 gtgggataaa tatatctata gaagtaaaga agtattttta actggtagtt ataacaaaac
     5341 tcttcaggag agcctgtacg acacaggagc aatatttaat aatgaaccta tggttttgaa
     5401 agatttcttt ggagattatg aactagtttg gaatccaaaa cagcttagtg aacagggggc
     5461 agaaaatgaa tttgctgaag ttcactaaca tagtgtacag aaaaacatta tctgaaagaa
     5521 gaacgccttc aaaagaaagt acaagaacga ttgggccgta cagaagacta acgaaagatc
     5581 gtaaaaaaaa ggaaagacaa tgaaaattaa gtagttcagt tttggagtac aaaatgacaa
     5641 aatgtacttc aaaatggact acttttttca ttttaactta cataccaaat tacatacaaa
     5701 atgatttata aaatgcaatt gaaaatgata attgaatgaa aatttgtgat tttgtggtat
     5761 tatgataata taacttatag aaaaaattgc ttattcaact aacgggcagg ataatgagaa
     5821 aaggtttttt gatagaaata agaaattaga tggtaaatta gttaaaaagg ctgtgttata
     5881 aatgaaagtt tctttgattg ctgcgatgga taagaataga gtgattggca aagagaatga
     5941 cattccttgg aggattccca aggactggga atatgttaaa aatactacaa agggacatcc
     6001 gataatatta ggtaggaaga accttgaatc aatcggaaga gccttacctg acagaagaaa
     6061 tattattctg acgagagata aggggtttac ctttaatggt tgtgaaattg ttcattcaat
     6121 agaagatgtt tttgagttat gtaaaaacga agaagaaatt tttattttcg gaggagaaca
     6181 gatttataat ttgtttttcc cttatgttga gaaaatgtac atcacaaaaa tacatcatga
     6241 attcgaagga gatacttttt ttccagaagt gaattatgag gaatggaatg aggtatttgc
     6301 ccaaaaaggg ataaagaatg ataaaaatcc gtataactac tattttcatg tatatgaaag
     6361 aaaaaactta ttgagttaac gggtgcgtta attgaagaag aacagtaaag ataaagcaca
     6421 agagcttggg attactccaa gctctttttg tcatttgaaa taaattatat ctttccaatc
     6481 aaattgttta tctgtaatcc ctaatctttg agcaggtgtc aaaacctttt tatccgctga
     6541 tttaaaaggg cgacaaaagt tgtagtaagt tcttagaatt gtaatcgcaa attgagcata
     6601 cttcggattg aagttagcgt aaatatagct cttcttatca ccacgagctg ttgttaatgg
     6661 tctttcaaga gatgatatat aacgtcttat ctgttgaata aagctattgg tcgaatggtc
     6721 attaacattt aatagcatct ttgcgatttc gtttggttcc agagcagata aatccgttct
     6781 acaatcaact gaataaaatc ctttatcctt agtggctaat ggatgtttga tagggttatc
     6841 ggcatatttg cgatatcttt ctccatcttt actaatagcc tcttcgtgga attgatgact
     6901 tgttagtaat tcagttagat aatgagatgc caaagtccgt agaaactttg ttttgaatcc
     6961 tctgatatct ccccaatcga gcagttctgc ttttgcttgt ccgaattctt ttaagcattg
     7021 ttttctgctt ttcgtcttgt taacttggca aataaaatga tgtgcatcgc tcaaccttaa
     7081 ttctttcgtg aatactctat aaaaggcgtt tctaatagag aagtcatcat cactaatcat
     7141 tcgccattcg tcggcattaa ccatttgttt aattaaccaa tagtgagcag ttgtagtata
     7201 aggagcatca acatttaaac catcgacaaa tctactccta ttagtaattt tccctaattc
     7261 gtgaagataa gcattcctat tttcgctatc gttagcggat ggttcttgtg gataataaga
     7321 ccaatctaac ctatcatttt tacgactaaa agtattgaga tggtcttctt tgaattttct
     7381 tgtatcttca tttaattcat ccattgaaat attccaatca taggcaacat cagaacggaa
     7441 cacatatctt gataagacct cagcagatac aactatatac gtttgcatat taaggtcttc
     7501 aaaccccgta tatttctttg aaccttgtcc tttcttacgg acgttattta ggtagtagtg
     7561 catcttgtcc gtattaagcc acatctcgtt aaatttcttt gtttgaaggg gttgtgtctc
     7621 atatcgttct aaaaactcca aacaacgcct atacagccac tccagcttat gatagtaggt
     7681 tccaactcca attcctaaaa tatcacaagt acgactgact gaaacccttc caacaaccat
     7741 cttagcgaac attggaagaa tggtgttctt ttgttggtga taagtagtgg tttcttctct
     7801 ttttggtaga acattagtga acttcttaca cgccttacat tgatagcgtt gagatttgcc
     7861 cctacttttc ccccttttgt aaaattgctt cggctcattg aatggggttg attcttcttc
     7921 cgagcagcct tctttatgaa attgataatc tggctcaaca tcttggatgc tattgatttc
     7981 tataaggcgt ttaatttctt caacaacaga ccaattagat aaaggggtta ctgtacagtt
     8041 ttgcgataca cctctgccaa taggattgtc attgcagtac atacctttat gacctttatc
     8101 tttggaacta ccagtcattt tatatcgact tggtttccct tttacatctt tgaatttgtg
     8161 ctgttctttc ccaaaattac aacaaaacgg attgatgcag taattatatt gaatgggata
     8221 ttcagaaccg ttccatttaa aagacgttgg caagaacaac attttgttat accttgttgc
     8281 aaatcgtttt ggagttaata aatcatattg tttagcccta tcttttaatt cttcgtttga
     8341 aatcggaatt ttaacatcta caatttcatc ttccttcgtg gctaatcttt taagcttggt
     8401 cattgtctat caacccactc tatctttgat acccattttt tcaacaatcc gcttattact
     8461 tcgcttgttg gcaatccatt caataaactt cttatcgtct aaaatgttca ataagaattc
     8521 atccactgct tcgctttcag tacgctctgc atattcagcg tattgcttaa ctatcaccct
     8581 tacttgctct gatatttccc aatccacaga cttagcgttc ttattttttg gctcaagaaa
     8641 ttgcatagga aaatgcaccc ctttttgctt agtaaattgt aaccatttta acacaccaat
     8701 ttgcattcct acaataatta tttcacgttc gcatttttat atacatttta acttacattt
     8761 tgtggtgtaa attaacaaca aacgttgatt taacaataaa aaacgagtat caaattcagt
     8821 tgcaatttga tactcattta ggtttacatt ttcacttttt gtactccaaa actgaactac
     8881 ttaaatgaaa atgtctttcc tttttttttg actacaagta gcctgaaaat gaaatttcaa
     8941 aagactacaa gtagtctttt gtggcaaaaa taaagagcca acgaaagcta ccaacaatcg
     9001 ttgactcgta agactgttcg tagtaagaaa aatctttggc aatccacgaa gtctttattt
     9061 cataatttat ataaatgatt atacagtcac tttcctgaat ttgcaagtaa aaaagctaag
     9121 aaatattgtt taagcgtttc cttaaaagaa tctttaaata aatttttttc caattgaaaa
     9181 catgactaca agtagactat ttcttttatt ccaagttgac tacaagtagt caaggtgata
     9241 taatatatat atcaagaaag gagaaacaaa aatgtataac gtaggagatc aaatagatga
     9301 gatcgtaaaa ataggtaatg ttaaacttgt taaaagtaag tactatattt cagaaactga
     9361 aataattttt aatactgaat caggatgtat tctaatttgg aaactgcctt ttcccaaaga
     9421 cgaaacaaca ctgaaaaaaa taaatacttt aaaaatcaga tattgcattc atgaaattat
     9481 tgataaaaat acttatgtta taaatcaagt aaaagtgatg gggtaaaaat cttttaaaaa
     9541 aactcttgtt aaaagtaatt ctattagctt ataatgttgg tattagttct gaagccaaag
     9601 gaacactaaa aaattctagt agaaaagtta gttacagact ctctactatg tatgaatgta
     9661 acgaaaaaaa tagcgaaaaa gataattaaa taagactacc aatcttattt aatggaactt
     9721 ctgtagtaag aaaagcgatt tggcaatcca cgaagtccac caagatgagc taccaactca
     9781 ttttaatttt tgcacgttta aagtttagca tttaagtgct tactctaaag acatagtgta
     9841 attttaggac tccttattac ggaatctaaa gttaccctat gtcttttttg ctattcaaaa
     9901 ttgatagcaa ggagaaagtg caatgttgaa tagttgcgag tatgcggacg ttttagctaa
     9961 aattcttaaa aatggaatta aacagtttaa tagcaaaaat ctagttgctt ttaaaaagaa
    10021 tagcaataag attggtgcta ttgccggatt tacttctaaa acaaacatga taaagactaa
    10081 aggtcttata tatcgttcta ttgatcgtgt cttggaagat tcagatgtat taacacactg
    10141 gactcctaac gtgtattcat ggcttggtgg cggtgaaaac aaaacaattt ttggtcacaa
    10201 agaagctaac ttaattcaga ttaatacttt tgtagcagat attgactatc cagtaggaga
    10261 aaagaaaacg gatttgaatt tactagttct ttatctattg gaagaagggt tattgcctac
    10321 actgattgta gatacgccaa aaggttatca ttgctatttt ctgattcaaa actataacca
    10381 agaaactgat tcatttgata aggcaagcta tatttctaat tcaaatgatt ttaaatcttt
    10441 gagagttgct aaaagaatat cagaaaatat cagaaaatct atccaaaatc gtttaccaca
    10501 agtcgatatg ggctgtaatc attttggtat ttttagattt ccaactaaaa aaaatattgt
    10561 acattatgaa cctaatttcg tagatacgtt tgaagggtat ttgaaatggt caaaagactt
    10621 tgaagaaaaa gaatatgctt taaagaggtc taacttgtca ttattagctc ctaattctac
    10681 tggcaagaaa gggtctaaac gacaaattga tactaaatgg tacgactttt taattcatac
    10741 ggatattgct gaaggagata gaaataaaac tatttttacc ttagccctgg catgtaaatc
    10801 aagtggctta tctattggcg agtgtatgga tcaattggat caacttcaat tcaataatga
    10861 tttgtcctat tctgaagttg agagaacaat aagaagtgct tatcgtggcg attacaaagg
    10921 tgctaaaagt tcatatgtca atgaaattat cgaaaattat gctacaccta ccgagtttga
    10981 aaaatactca ataaatgtaa atagacaagc tgttaaacgt aaagggaatc gtgtcgatcc
    11041 gtctaattgg gtgaaatttg ctaaacctag agaagaacga aaatatagtc attttgaaga
    11101 aagtaaagct gatttattag cttacttaga aagaaaacaa agatcattga aaaaaaatga
    11161 gttgttttta agtgtctcta tgggaactat atctgaagaa acacaaattc ctgtatctac
    11221 gttaaaggtg attttaaaag agcttagaac ggcgaataca gtgatattac gaacgtctcg
    11281 tggtcggtat ggatcgacta aaatagcaac aagcaaattt gtggaagcta aaatattaaa
    11341 tacagttatt cagaataaaa aagataaaca agtatttgtc ttagaattat taggaacagc
    11401 aaatagccac gtagaaacga ttttaagacg ttatgaagac aaaacagtac cgttccatgc
    11461 gaacatactg aaagaaagag aaaagagcaa acatgcgcca attagaggag ctaatacagg
    11521 ttagagttct atttattttt ttgattttta aatagctaaa ttgccaatta acatatctag
    11581 tgtttctgcc cactcctatt cctgttttag cgtatactaa tgattagtag ttagtactct
    11641 cttttatgta tggagtgtat agaactattt ttacttgtgt cttttccctt aatccacgtg
    11701 tacggaatac agcttataac tgcgtatgca gttatgtttg aaggaggaag attttctttg
    11761 gtaattaaga aatatgttta ctcacacccg atcaatgtat ttattatcgg taagctaggt
    11821 ttatctgttg aggtgttttg tgagttatat ggatttaaac aaggaactct atcgtcttgg
    11881 attactagag agcgtgaggt agctgatttg cctatttctt ttttgtatgc tttgtctcta
    11941 gcttcaggag ggaatatgaa tgaggtttat gattgcttgt gtgagtatga aaaagagtat
    12001 aaagagtatt tgaaaatttc agccccaaaa cggaataaaa aatatattga agattagaga
    12061 aagaagaaaa gaataatttg ttttttgact acaagtagac agatagctac tctgtttttt
    12121 gatagtttga tattggctgt ttttttgtta gaattaatat atatcaggta aataaaaaag
    12181 ggggctatta atgaatattg atgggattta taatgaatca gattttaaaa aaggcatagt
    12241 agatattgat agaaaagagt atttatttcc tgatgaaata ggtttattta aaattgtagg
    12301 tgaaactgtc tgtaggtatt gggggaagaa aggaaatata attttacttg ttgatacgat
    12361 tgagggaaga aagttaaaat tttcgttttg gagtaataca aaatattgtt gtgaacctgt
    12421 acctaaaagg gaagtagatg tggctaaaga aagtgtacga tttttacctg atcgatcaaa
    12481 aataacttta atttgtgaac ggacaagtaa tgctaaattt acaagaatta gagatatact
    12541 actttcgact acaagtagtc agtcatagag taagaacttt tggttaaaaa tcaattgaag
    12601 aaagtggagg aagtatatgg cagtagttat attagcagaa aaaccagatc aaggagcaaa
    12661 atttgcttca gcattggccg gaaaaaaatt atcgaaaaag agtgggaagt atgaatttaa
    12721 aagtgaaata tttggagata ctattgttac ttggggaata ggtcatttgg taggattatc
    12781 attacctgaa aagtatgagt ggctaccgaa taaagataaa tggcatttag ataatttgcc
    12841 ttttctacct aaattcaatg aattaaagta tgaagtctct aaaggaaaaa accaacaatt
    12901 ttcaatagtg aagtcatgtt tagaaaatgc agatgagata ataattgcta ctgatccgga
    12961 tagagaagga gaaaatattg cttataatat tttcaaatta tgcaagcagt cgatttttaa
    13021 taaaccgata aaaagattat ggattaattc tttggttcaa aaagaaatta tcaaaggatt
    13081 taaaaatctg cgaaattctg aagaaacgat ctcttacttc aaagaagcta atgctagaca
    13141 aattgctgac tatctagtag ggatgaatta tacagagttg tattcattaa agttgcaaga
    13201 aaaaggtata tcgggtgtgg tatcactcgg tcgagttcag acaccggtaa acacacttgt
    13261 ggttgaaaac gatttggcaa ttagaaattt tagaccggaa aaatataaaa cgattgaatg
    13321 tcatacaacc gataagcaac caaaagcaat atttaagaat agtcttgaat actttaaatt
    13381 agaaaaattt gaagaagaca ctaaaaaatt cggccttgat tccgccagta ctggaatcat
    13441 aaaaaaaatt gaggttagta aaaagcaaac agaaccacaa aaattgttta gcttgggtgg
    13501 tattcaagaa tacgctaata aaaaatggaa atattctgct aaaaaaacag ataaaattat
    13561 ccaaagtcta tatgatcgtg gatatttatc ttatccaaga accgatagtg aattgataac
    13621 tacaaatgaa tttgagtatt taaaagcgaa tttagacgtg tataagagtg ttttagggat
    13681 cagtattgat acaccttgtt tagagcctaa aaagcggttt gtagataatg acaaagtatt
    13741 agagcattat gcactaatac caacaatgga tattcctgat atagcaaatc tgacattaga
    13801 tgaaaaaaat atttatgaga tcgttactag gcgtgcgtgt ctaatgtttg cagaacctta
    13861 caagtacgaa aatacaaaag ttacattaga tgtaaacggc ttagaattta ccacaagtgg
    13921 taatgtacct atatctttag gttggaagca agtagatact aatttagatg aatctgatga
    13981 taaagaggaa acattaccta aatttcatga acaagagaca ataaatatag atattagatt
    14041 tattgataaa ttaactaagg aacctaaaag gttgactgaa ggtaaattat taggtaaaac
    14101 cggtattatg gcgaaattag ggctaggaac gcccgctaca cgttctaata ttgtagacac
    14161 tcttctaaat agggaatata ttaagataga aaacacgaat gtttatccta ctccaaaggg
    14221 atatttatta tgggatttga caaagaataa agacctattg attggaaaac cagaaaatac
    14281 agcgatatgg gaagaatcac ttgggaaaat tgcgcaaggt agctatacac aggaagcttt
    14341 tttaagaaat atctataact atatcaataa gactgtttca gatttgaaaa aagtagaatt
    14401 tacttcggat tatttagaaa agactgtaaa tcaaaataaa tttgatatag gtagttatca
    14461 agtggaagaa aagaaaaaag tttattctat tttaaatatt actagtggtg aacagttcgt
    14521 gatatttaaa aatatatcag gaaaagcaat caataaaaaa gttcttcaag aactattact
    14581 aaataatcgt acagataaag tcctttccgg ctttaagtca aaagaaggaa aagcttttaa
    14641 tgcaagatta cttttcgatc caaatgtgat gaaagtggta atggagtttg aaaagaacag
    14701 tccaaaaggc gataaaaaga gcaaacacga tacagtaaat ggttatgaaa tagttgaaaa
    14761 agcaaaagta tttgaaataa aagaattagc aacaggtgaa agttttattt tctataaaaa
    14821 taatagtggg aaaactttaa gttcaaaaac agtcaaagaa ttgttagaaa atgggaaaac
    14881 taaagagaaa gtaacgggtt ttataagtaa agacaataaa aaatatagtg cttatcttgt
    14941 ctttgataat aaaaacaaag ttataaagaa agaattttaa ttcttagtgg cggaaatcat
    15001 ttgtttttcc gcttattttt attcaaaagt gcccaaaata taggtatact gaattaagga
    15061 taaatacaaa actagttaga ggacagttga aaaaatacaa acaaattaga ttgctatttt
    15121 gttaaattgg aagtagaata ataccgtttt gatatgtata gtgtgtttta ccaataaaat
    15181 tacataaacg tgaatatatt tttatgaatt aaatgtaaaa tgagagcaat tagttttaaa
    15241 aaagattata ggctgttaat cttagcatat ataatccatt ggaaaggggg agatccaatg
    15301 aatctaaaga atacaaactc aaacaaatct acactatatg taatttcaga tgattatata
    15361 aataagctga aaaaggtgga ctatcgtgtc caaaacaact atcgagaaca acgattatat
    15421 tttaaatcaa acttgaaaat ttctaacaaa aatgaagaaa taaattatta tgtaccattg
    15481 gcttcagcaa aagaaaatca acgttctttg actaataaat cagtgtttaa gatttatggt
    15541 gatcgagaag aaaaggactt tttaggagta cttcatataa ataatatgat tcctgtgcca
    15601 aaagattgtg caaaagtatg gacacctaat agcccaaatg ttgatgagaa gtataagatg
    15661 ttagtaatca aacaggcaaa atatttgaaa ttacatgagt ctgaaataaa tgaatcatgc
    15721 gaattacttt ataattctaa gttaggtaaa cttgatccta gttactttaa gaaaaatcgt
    15781 aaagaagtaa ttacttatca aaaaataatg acgaatgtag aatcattgga agctgttagt
    15841 atagcagaat cccaaagaaa tattcatgta caaactcaaa gtatagatgt agagtaagat
    15901 aggctaaacc tatcttattt tattattcta ttggatatta tgatacattg aattttaatc
    15961 gaatatgtat ttaaatgtta ttatactgta gaagataaaa cttaaaggat gatttctatg
    16021 gcgagaccga gaaaatacaa tacagaagaa gaaagaaaag aagcaatgaa aaaggctagg
    16081 aaaaaatggg ttgaaaaaaa tcaggaaact attgcctata taagagccaa atcatttgct
    16141 aaaaaattca ttgaaatgtc tgaagatgaa gatattgaat tgttggaaaa atggttagaa
    16201 gatcggaaaa ataacggtca aaatgatgaa taataaccat tgactacaag tagtcaatgg
    16261 tgtagaatgg ttttagggat agaaatatcc cttttctaaa agtctaatga ctacaagtag
    16321 tcgatagagg gatcgggaat aaaaacagac gattatggcc taaagaaagg aggaatatga
    16381 atattttgag gaacgattta ttaaatgagc gttgataaat ttcactaaat attttcttaa
    16441 tatctatgta ttgttcaagc gtagaaaaaa atattgaaat tattacaaaa ccgctcataa
    16501 tggaaaataa cgaattatat taatgaatcg ttcacttttg acctttttta ctagattttt
    16561 ttgtaggact tttagagaat ttttttgtta tctgattata aggaggagct aaagttgata
    16621 gagaatagag tactggggtt cgtagagtgt aaagataatt tagacgatac tttagaagtc
    16681 cttgaaagta ttatatttaa caccaaagtt ctggatttga aagcaattaa tggtgatatg
    16741 acaacacata tcatattaga tgaaattagc gcaaaagaga ttggcgaaac tttaatagca
    16801 tgggctaata aagagctgta aaagataatt ttaaaataaa gtggccaatt gactacaagt
    16861 agtctgtata gtataataaa ttatcaaaag tgaggtatac atgatgaaat caaatttttt
    16921 gggatatgta attgcaatca acatgtttaa agggggagtt ggtaagtcaa ctcttactca
    16981 gctaatggct gatattctat cggctagagg ctttaaagtt ttagttattg atgttgatcc
    17041 acaaggaaca gtaactaaaa agtttcaaag acaatatgat ttaaaagttg ttgggaaaat
    17101 gacattgttt gaagcagtaa ttaatatgtc tttcttagac tgcatttctc ctgttaaaga
    17161 aaacttagat attgtgcttg gttcagaaca aatgtcaagg tttaccaaag tattaagaga
    17221 tatgtggaga agtactggtt ttactgatga aggtgaagaa gtcttttgga atttgtttaa
    17281 ttatatgatc ggtaatgata atttaagaga tcaatatgat tttattttgt ttgacactat
    17341 tcctacagtt tcagaattta cagataacgt ttacgtggcg agtgattttt tagtaattcc
    17401 cacgcaaact gagcaagatt ctatagacaa cttgcatagt acggttacta attatcaaac
    17461 tgctgtaaaa gaagtaaacg gagatttaga aattttaggt attgttccat atctagtgga
    17521 tactaaatta aaaacaggat tagaactttt atcagaactt caagaagaat atggagactt
    17581 agtttattct catattgttt cagaacgagg cgtaattaaa cgttggggta gagaaggact
    17641 aaataaatct atcccacatt ctaaaactgc ttatagcatg tatgtagaca ttgtagaaga
    17701 aacgttagta cgtttagaaa aagctacagg agaaaatatt ttagaacgtg tttaggaaag
    17761 gtgttgcatg atgtcgaaaa ctaaaaaagt aactagtgcc ggcagtaatg gttcgggtaa
    17821 tccgttcgga ataaaaagcg gtagtggtgg atttaatata ggacaacaaa tgttaaaagg
    17881 taaagctggc attaagtcta ctggtgataa acggaaatct gttagggtac cagttgaatt
    17941 atacgatgaa gttgttaaag atttatcata cggggctaaa tattcattaa tgacagaaat
    18001 tttagcttta tttttaaaag aatttgaaac acatggaaat agtatagtta ctgattttgg
    18061 caagttgaaa acgagaccag taaaaatttc tgaagaagcc tttaaagttt tgaaagttat
    18121 aaaatttgaa acagatcaat ctaatactaa tattattgct tcagctttga catggtatct
    18181 tgataaaaga aaaggaaaag cataatgaaa tgaggtaaga ctatggatca taaatcaaat
    18241 tattcataca aagaaaatta tatttggaag tatgtagtag ccgtttttct actggtaggt
    18301 ggaattattg cattggttaa atgggctata attcctaatg ctttagtatt tatcaattgg
    18361 tttgtcccta atttttggag tttttttggc tatatattga taatctggtt tatgtttaaa
    18421 gtactctctt ttttctggtc gcctgtatat tctttgcttg gaaagtggtc gtacctaatt
    18481 ttaatagcga cagctattta tatcattatg cagattaact aattatgcag attttagaag
    18541 atattaaaat ttattggaaa aggataatag gctatacatt agttcttctt gtagtaaatt
    18601 tattcctttc gatgatagcg ttggtctttg ttaatcaaag aaaagacaat gtaactgaat
    18661 tagtaacgtt tatttcgttt ttcataagtg ggatttttgc tcctttcttt gaagaaacct
    18721 taatgagagg aattatccaa aaccaattgg aattaaaaac aaatttgtct acaagaagca
    18781 tatatataat tgttgcaatt ttgtttagtc tatcccactt aaatttatat tttattcctt
    18841 attttatgac gagtttaatt ttgtcttttg tctatgataa ttcagataaa aggctgattg
    18901 ttccggttac tattcattgt ttttacaatt taattgtaat actgttaagt gtagtttttt
    18961 atacttgaaa aaaatagacg aaaacctaaa aatacaaggt ttttatctat tttttttatt
    19021 ttgtttttat ctatcaggtt gatagaaatt gtcataaatg atataattaa gtatgaactg
    19081 atttagtaaa aaaattaaag gagtgatagc tgttggcaag atcaagaatt gctaaaattg
    19141 aacagactaa aaaggataaa aaaatttcta aaaataaaac aaaattcttt aaagctgaag
    19201 atgttaagag gatagcatgg tctgtgaaaa catttgatga agatcgttat gttaaagttt
    19261 caatgaatgg tctatttgta cagcttggta acttcagtaa ttttaaaacg attgatgaac
    19321 tttatccgga aataagaaat attgtggata gtaaaggttt aatcgtggct aaacggaaat
    19381 caagaaactc tggtcttgct tactgtaaaa ataaagggat catgattccg gcttaaatca
    19441 gtgaaattgt tctttgaaaa cttaatacta ctcgtactgt agcaatgttg ctatgttaca
    19501 gataaaagcg taaggctaat aagcaaaata gcaaccaagc atactgttat ttcctttgcc
    19561 aagaggtatg taaatacgtt agcaatagaa gtgataaggt tagtatttca cgccaaaaaa
    19621 tacaaaagtc gatccattga aactactaat tatgaggtgg cgtaaatggt aattattcag
    19681 aatttctatc taggaagaac ctatgaaaac cgtcctagag atcagaatat tgacactaaa
    19741 aaattcagca cagtaataaa tgtgctagat acagggtaac tctttactac aagaaatgta
    19801 gtaaagagtt gttatgattc tttattacat ttctttctaa aggaagtgtg ataatgaaaa
    19861 aactaaaact gattagcatt gctctattca gcctaatcac tatgtcaacg gctcgccaat
    19921 caattgtttc ggctgaagga acgtctacga aagcagtagt agaagagtct ggcgataaaa
    19981 aagttgctat agacgacaaa gtgaaaaacg aagcggaaaa agcgactgtg gttcaagggg
    20041 cagacggctt gatgtatgtg gtcgttgatg aaaatgatcc tgaaaaattg ctagaagctg
    20101 aatctccttt gaaaaatcaa caagaaaata ccggcgctgt tgaagtcggt gttgatgaaa
    20161 agagacaaga aaaggctgat aaagctaacg aaaaagtgaa aaactttgac gatattgatt
    20221 acaacgatat tagttcgttg cagtcagcgt tgaatgatcc gaaattccct tttttatcgg
    20281 aagatgaatt aaatgaaaaa ttgcaaaaga caaagaatac atttgagtct gaagcgctaa
    20341 tgaaaaagac aaggggatat ttcagtcaaa ggattttaga gttaagcaat aatttaaatg
    20401 acgtaccgaa aagagcgaaa gtgagtcggc atgctttagt tagtaaccgt gcttcacaag
    20461 taagtgaaac acgaataaac cctccaaatg gttatccgtg ggtgatttat ttaattagga
    20521 gcaatggtta tagttataca gaaaattatg ctattagaag aactattgat gggaaagaag
    20581 ctttttgttt acaggccggt gtacaagcga cgaatacata cactggttca acaagtggtt
    20641 atctttctaa ttcgcaaaga gaaatgatta gttcgatttt atcagcatat aaagaattag
    20701 gcggttgggc taataatgat gccggaaaac aaagatggat agcaactcaa atgcttgttt
    20761 gggaaaacag taatgaaaag cctaaaagta taactgttac gccaaattgg aaaaacggag
    20821 ctactaagtg gaaaaatgat ttagtggctc gtgcaaatgc tattagagta agaacaaaat
    20881 gggattctac gtctaagacg gttgatgagg gagataaagc aacctttaaa gtaactaata
    20941 cttatggaga tcataatttg catgtgaaat ctgtatctgg tggcggcaaa gctaccataa
    21001 gcaatggtac tttaactgtt gatactacag gagccactgg aaacaaaatc actgtagata
    21061 tgattaaagg ttctcaccct tcagacgcgc accctacaac ggtatggagt aatggttctt
    21121 atcaaaagct tgttaccggt tctttttaca ttagtacttc tgttacggta aatgttaaga
    21181 gaaaaggaac tgttgaagtt ttaaaagttg attcagaaac aaaaaaacca ttatcaggag
    21241 caacatttag atttagctac tcaggtaaaa caaaagatgt tactactgat tcgaatggta
    21301 aagcaaaatt aactgaaagg ttgaaagctg gaactgtagt aaaagttaag gagataaaag
    21361 ctcctaatgg ctaccaacta gattcaagcg aattttcaat tacagttaaa gaaaatcaaa
    21421 atgttactac cacaagaacc aataagaaat ccacaggttc tgtagaaatt gaaaagatag
    21481 gcgatttaga tggattagga ctaccgaatg tagtattcac gatttataat tccgataaca
    21541 aagttgtaaa agataactta aaaacagata gtaatgggaa aataaaagtc gatgaactgc
    21601 aatttggtaa atattatgca gtagaaaaac aaggtgttac tggttatgat cccgatggta
    21661 aaaaatacaa ttttgaaata agtcaagata ctccttttac aaaaccggct aaagtgaaag
    21721 taacaaatat ttatgaagta tccataagcg aaaaagtatc ggacatgaat gaaagtcagg
    21781 tattaaaaaa taattggtct gctgatccaa acgattatct tcaatatgac attacaactg
    21841 gaaatctaaa aaaatcggga gaaagaaacg tccaaagttt cattatcgaa gggttttacg
    21901 acaataagaa tgtagatgtg tcaaaaagtg aagtatatgt tggatcaaca aatgtaacaa
    21961 actcttttga cattaataac gatactaaca atggaaaagt aattgctaag gcaaagacaa
    22021 gcgtattaac gaataatgat ttttataata agtcctataa tttacggatc actatgaaaa
    22081 ttaaaaaatc tactcttgaa aacactgaaa agcaaaaaaa tttaacgatt gttaaaaata
    22141 gtaaagttac tgtcggttca aaaatctcta aatctgctag ctctaatgat gtagaaaccg
    22201 ttttatattc aagaaaagtt actatcaatc atattgatga aaaagacaaa caccttttga
    22261 aacaagatat tgattataaa tacgatgggg aaacgtatga atataaacct agaaccgatt
    22321 tatttgataa agatggaaac aattataaat ctgatgtaat ccataaaggg aaaattgatg
    22381 gaaaagatat agttttaaac actccctatc atattccggt actttctgtg aatgtggatc
    22441 ggattcaaat tgatacggct agagctgtta aaaatggcta tttacctaca aaaatagaac
    22501 taagtaaaaa agaagaatat gaaaaagaat tagaaaaaat tgtattcaaa gttaaaatta
    22561 cagatataga taacaataaa gttgtttatg atgaaaactt taaatacaaa gaatttaata
    22621 gtcatatgtc gcttgattta cctacagaat atttaacaaa gaatgtcaat ggccattcaa
    22681 aaatgtaggt ttttggccat ttagaaatgt cgttttttgg ccaccaaaaa tgtaggtttt
    22741 tggcccataa ataacaaggt tacttatacg acaacttttt ttctttataa tctttaattc
    22801 gataagactt tccagtaatt tgaataatct tagcgtggtg cactaatcga tcgattagtg
    22861 catttgttaa ttttttatct tgaaaagttt cagcccattg agacaaagga atattcgttg
    22921 taataatggt tgattttttt tcatagcgta gattaattaa ttgaaagaat aactttgccc
    22981 cgtcaggggt aaaaggcaaa taaccaattt catcaataat tagtaaatcg taagaagcat
    23041 atttttttaa caatcgatct aaatttccac gttgatttgc acgatttagt cgattgacta
    23101 attcattgct taaagcaaaa tggcaagaat aacctttggt tatagcttct agtgtaatgg
    23161 caatagctag gtgagttttc cccactccgc tatttccaat aaacaatata ttattatttg
    23221 tatccataaa gcgtaaggta cacaaatcta aaatctctgt tcgattaatc tttggttgaa
    23281 atccaaaatc aaagtcattg attgtttttt taaacggtaa attcgccttt cttaaacgat
    23341 tttctttttg tcgttcattt cgtgagttta tttcttccgt cattaaatga tttaaagctt
    23401 ctacaaaact aagattttct tgaacaatgg tatcgagata ttctggtaaa atttctctcg
    23461 tatgttttaa attcaaatct tctaatttgt ttagtaaatg ataataatta gacataatga
    23521 ttcacctctc tataattcgt catacgcttg taaattttca tcaacaaaac gttctaattc
    23581 ttcgtcttct aaatgtttaa aaacgtcaga acgtagaatt tctatatagt catttcgatg
    23641 ataattaagc aattgatgag tcatcgtatg tgtgcgaata agagtagagt catcgtaaat
    23701 ataaagttta tcttctttta cacgaagtgt aagaatttta ccgatgtaat ttactggcac
    23761 agaatattta ttttgattcc attgaaccat cgcttcgtta gatacttttc gtttaatttg
    23821 tttttcaaca gaagcataaa ttttaagttg attcgcatca aaagaagata gttgttcttt
    23881 ctcgtatgac caacggtcac taggacattc atgaattgct tgagaaattt cttcagtatt
    23941 taatgaatcc atgattgttt gaataatttt ttctaattcg tgagtagatt caaattcata
    24001 attaaataca cgaattcgtt caattgttcg tgcaagagct tcaacttttc cttttgtttg
    24061 aggtcgaaaa gcacgacaag caattggaag aaaacctgct tctttcgcgt attgtcggaa
    24121 ccgctcatta aaaataactt tagaaaattg actacgacta cggtcaacaa cagttttcat
    24181 attatcaaac catatttctg taggaacacc tccggtgtat tgaaaagcat tagttaagca
    24241 acgaaataaa gtagtttgag aacgatcaaa agttagttct aaatacttaa ttctggaata
    24301 tcctaaaacg taaaggaaga tattacaaga aataacttca ccgaatttat tgactaattt
    24361 gaggtcttct ttccaatcaa cttgagcaga taagccagga gttgtttcaa cacgaatcgt
    24421 tgctttttgt attttatctg ctttgatatt gcgacaatat cttctcagtg ttcgtgcact
    24481 tccacgatag ccacgtttag aaataaaatg ataaatagca gaagaagaac aacctaaagc
    24541 taatttgtca gcgataatat cttcaaaatc actaaccaaa gtttttttgt ttttattctt
    24601 ttgagcaagt aaaggtaatt tattgcttat tccagcttca taataacgtt ttactgtacg
    24661 ataatcgcaa ttgtattgtt tagctagagc agcataatta ggttttgtat gagtcataat
    24721 gaaaggtaac actccttcat aaatatcttt tctcaataca ctttcctcct tgaataaaat
    24781 aacacaagga aagcgtacca gatgacgcat aaacctacat ttttatttgg ccatttttct
    24841 acattttaac ttggccattt acaacaaaga ataaaaaaat caattatagt gttgatattc
    24901 ttttagtgga taatccagat aaaaacaagt tcgaaactga aactaaagat ttaagaacga
    24961 tcggttatac ttcttctgaa aaagttttaa caaacaaaga tatgacaaat aaccaagtca
    25021 actattcagc agtagttaga acagtgaaag aaagagagaa taataatccg gtcaaagagt
    25081 tcaatgagag tatcaattat aaatttactg atagcactaa aacaaaaaca ggatatggta
    25141 tatcagttga tttagagcca acatatagaa atgaaattgg gaaacaaata gatatgaaag
    25201 tctatagtta tccgatgaat gaattgattg acagcaaaat tgacgaaatc tatcaaagtg
    25261 ggggaaaaga aacagaagtt aagatggatc gcttaaagaa tgatggtaaa gttgagtcgg
    25321 gtattagtac tcaaaatctt tcattccaat ttccacatat gaatatcgaa agaaaaactg
    25381 gaaatatata tgctgacagt caaagaaatg atgaatctat taagtatgaa ttgatcgatg
    25441 gcggtagaaa attatatgtg cctatttggt gtgatttggg aaattattct attgctatca
    25501 aaagtagtaa ctttggtggt aactatatct cgttggatat gaaccagaac atagatgttt
    25561 atgcttatat gtatgcaacg atagatagtg aaacaaaaaa agatgatgaa ttactgcttg
    25621 aacctgttta tccagagtct actctaccag atggttggag caaaaatgag attgattggc
    25681 tgaataaata agtttgaaaa gtttttatct atctgataga attaaataat gatataggga
    25741 tggtgataga atgagggttc aaagctatgg taacagtgtc tttgatgtta aaagagaatt
    25801 tcaagattct agaagaaaaa ccaaaaagct gatagcttac ttcattctat cagctatcgt
    25861 tttctatatt gtcaagtttg ttttggaatt aaactcaata gtattcacag atatggttca
    25921 taaaatgtta aatggagaac ctattaattt caactttgat tacatgcttg aaaaagcaca
    25981 aagcaatcaa attgtggctt ttatcgtgag tatctattat tttattgaaa aatctatttt
    26041 ttctttaaaa gattttttct ttgaaatgct taccaaacta acaacgtttt acaataaaat
    26101 ttcaaaaaat taaggaggaa gtttgaatgt tagatcaaaa tacagacaga agttggtgga
    26161 tgattggtgc cgtgatcgta ggtgccatct taatcgggac agctaaagta gcttttcccg
    26221 aagtgtttaa tatcgtcatt acatttttca aaaacatgat ccctacaaac ttaggatagt
    26281 tccactaaaa aattcacgta gtttgatgcg tagaaaaata ataaagaatg aaaaaggcta
    26341 cataaagtga tttatgtagc cttttttttg gatggaagtg atgtaatgct aagagatatg
    26401 cccaaaatga ttcttttaat gtttgttggg ttctttttaa ttttagtgat tgcgtactat
    26461 tcattaaatg gctatacaca agattcgtct gtgaaaggta tgcaagaaac gttaagaact
    26521 acagcaatag caaacaggga tgatagtatt cgagtagaaa aaggacgatt tgctttaatt
    26581 aagtctgatt ttgaaaaaga ttttaaagaa aaatttggga aaagtaaaaa tctaaataat
    26641 gaaaccgctg attataaatt tgattatctt acagatggga aggacggtat taaagcaatt
    26701 aaagtgaagg ttgaaagtgg atcaaaagaa tatcaagcaa cttgtatttt aaatgttgca
    26761 aatgattaga ggtgtgaaat ttgcgtgttg tagaggaaaa gaagatttct ccaaaaatcg
    26821 aggaaactcc taaaagtaaa attaatatcg caagtattat taaaaaaaat ggaattaaag
    26881 aaaactctga agcgatagaa ggaaaccttg attttttaac taagctttta gaagaagata
    26941 gtggaaatta tgaaccacca attgaagatg caaaattaaa tatgattcgt gatgatttaa
    27001 ccagaaacta ttcccaagaa gttttacaag ctttaactga tgatgaaatt aggcgaaaac
    27061 ttgtacaagt tgttttgaaa gaacatggta ctttctttaa aaaagataaa gaggttgcag
    27121 aatatgtagt acgtgaatgt attggaacag ggattattga gcgaattatg accaacgatg
    27181 aaattacaga tgttggttgg aatggctctc aacttagcat tgaaactaac tctaacaaat
    27241 ggttgtttga cggtgataaa ttaaatatca cgcaagagta tattgaacgt gttatttcta
    27301 aatatgctgt tgtaaatgat cgggctttta atagtggtaa tcctatcatg gatggaatgt
    27361 ttaaaaatgt tcgtatatca gctacacaca tagataattc accagatggc tctacgtttt
    27421 ctatgcgtgt ggcaaggcct agattagcat tgaataaaga aaactttgaa gattttgctc
    27481 ctgattatat tttggagcta tttgagaaaa tgatgaaaac aaaagcaaat atctgtatat
    27541 caggggagac aggtacaggt aaaacggaac ttcaaaaatt actgctaagt tttattgata
    27601 atcatgatcg tatgattatg attgaggacg ttcaagaaac tcacgctaaa gaattatttc
    27661 cggataaaga tattttctct tggatcacta ctcccaaaaa agcaatttca gatttagtaa
    27721 aagcttctct tagaaataat ccacgatgga tcattgtttc agaaacacgt ggttctgaag
    27781 cttatgaaat gcttcaagcg gttttgtcag gtcactatgt tgtaacgaca cttcatgcgg
    27841 ttaatgcacg tgcgatacct agacgttttg taaatatgtg tgcaagtggt tttaaaataa
    27901 acgaaaatgc ggttgaggaa gacataaatc gttatttcga ttttggtgtc catatcaaaa
    27961 aaacagtggt tgatggtaaa gtgatccgtt atttgaatga aattgttgaa ttttctgcta
    28021 gtgaaccgac tacagttttt aagcaaaaat ttatcaatgg aaagtttgtc attgaaaatg
    28081 gtagtctttc cgaagatttt aaagaaaaaa tggctgaaaa atttattacg tttgaatttc
    28141 ctgaagatca aactaataca gaagaatcac ttcctgaaga agaagaggtg atttaagttg
    28201 aaagtacagg ctatgacaaa gcgaaaaggt ctcatggatg tttcggaact aaacgcctat
    28261 caagtagcgt atggtaaacc gttagattat agagattatt tgaactatgt tggattacct
    28321 tgtttgttta tgggtggctt ttcttttgct ttatgttact actggtggat tgctttgatt
    28381 tgtggagtaa ttggaggcgt atatggcttt aaagttataa tgcctaaaag tattaaacgc
    28441 tcttatgaat taaagagttt acaggaaaga aataaattta ttaacaacat gactcaaatt
    28501 ttgaccgatg atagtaaaac agttactaga gcgttatcta tagcaaaaca gcgaacaaaa
    28561 ggtgaattaa gagaagatat tcaaatctta gaatcacgtt tacaaggtgc tgataaattt
    28621 cagattgctg aagcttttcg tgaaatcaac aataaatatt atagtgatgt tgtttttaca
    28681 caatactttg agcaattgga aacagctatt tacgaaggta gaaataattc agatactatg
    28741 aagcaaataa agagctatca taatgatgtg aaacgtaaaa cagaaggttt tatgaaaatc
    28801 aaagacggct atcttcaaga tatgaagcaa atgatgttta ttgtagctgt ttttattgga
    28861 gctataactt ttgcttttgg tttttctact ttctatgaag gttttgctcg atcacctatc
    28921 ggatgggtat ttggatctat ttactttttg attatggtta gcttcatgaa gtcatttttc
    28981 aaacattatt ttgatgatga aataatgagt ttgggggtaa gtaattaatg aaagttaagg
    29041 ctttaaaaaa gaaaagaaaa tcaagtatta gccccttgaa aattacgaaa acagaacata
    29101 aaagcttttg gattaaatta ctaggtgatg tttattatca aaaattacgt gaaatgaaca
    29161 attccaatat tgcgattatt aagtggcaaa aacaacggtt aaaaaagtca ttgattttta
    29221 tcctagtcgg attcttaatg tatctatttt tagggaaaga atggtattac ttagccggtg
    29281 gaattgctac ttcagtaggt ttttatgttt ggcaagcaaa atcaatcaat ggtatctata
    29341 atcaatttcg gtttgaacga cacttgcaat ttagtaaatt tacacggcta ttgatcccct
    29401 atctgaaaca aaaaaaagat ggcggaaatt tatatggtgt ttttgcaaaa atcgtaaaaa
    29461 gactagatta tgataccgat aaaaagctat taatgaagct tatgcaagaa atgacagatc
    29521 agcctaacga tattagacct tttacggaat atgcagaaca gacttctgga acagacatgt
    29581 ccgtattatt tatgtctaca gtttatgata ttcgacaagg ttcggcagat ttggatgtta
    29641 ttgaagagtt ggatcgttta gcttctgaag agttaatggc cggaatcgat tcaatcattg
    29701 actataagtc gaggcgtttt gtcttctttc ctacaaaaat tgcaatgact tcatttattt
    29761 tagttgtcgg ttttgcagta ggcgttcttt tcatgaacct aaaagatatt aactttgggc
    29821 aataaaggaa aggagaaagt caatgcaaga acaatccgtt atggaaatca ttaaatggtt
    29881 tataggttta atgatgatta tggcaatggt ttctatagga cttttttgtg ttcaattaca
    29941 agatattaat actttcaagc aacaagtaaa ttatcaaatt gaacgaaaag gaggtctgac
    30001 gacagaagct gttgaagcaa tcgacaaata ttcggctact aattatgata gacgttttac
    30061 aattgaaagc gataagttaa atcagaaagt atcttatgga gaagaaattg attatacgat
    30121 caaagcaact attccaattc aaattttacc gcttcctgat gtaactttat cgtttaacgg
    30181 aaatagtgtt tctcaaataa gatgagagga aggataaaat gcaagagaat aatgtttcca
    30241 gaataattta tttgattggc ggattgataa ttttatcctt agctattgcc ggtcttgtag
    30301 agcattggcc ggaaataaag ttattaataa gaaactttta cgacaataaa atgattaaaa
    30361 tgtaggaggt atagatttgg aagcaattca agaaaatcgt aaggataatc aagaaacaag
    30421 tgaaaaaaac aaaaaaaaaa gaaaaaagaa agtagataaa tttacggaaa tgaccgataa
    30481 aatcattgaa aagaaaggac ttaaacctaa agactttgac cgtcaagcaa tcaaagaagc
    30541 acgacaaaaa ctgtatgatg attttgttaa agaaaacata tcacagcttt tagactaa
//
LOCUS       DACEND010000016        29965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEND010000016 DACEND010000000
VERSION     DACEND010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.017"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(266..1210)
                     /locus_tag="IW746_000654"
     CDS             complement(266..1210)
                     /locus_tag="IW746_000654"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1077577.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1506..2450)
                     /locus_tag="IW746_000655"
     CDS             complement(1506..2450)
                     /locus_tag="IW746_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077578.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2671..3885
                     /locus_tag="IW746_000656"
     CDS             2671..3885
                     /locus_tag="IW746_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1077579.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3907..5922
                     /locus_tag="IW746_000657"
     CDS             3907..5922
                     /locus_tag="IW746_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1077580.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6005..7378)
                     /locus_tag="IW746_000658"
     CDS             complement(6005..7378)
                     /locus_tag="IW746_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1077581.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7404..7688)
                     /locus_tag="IW746_000659"
     CDS             complement(7404..7688)
                     /locus_tag="IW746_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1077582.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7690..9705)
                     /locus_tag="IW746_000660"
     CDS             complement(7690..9705)
                     /locus_tag="IW746_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1077583.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9884..10189)
                     /locus_tag="IW746_000661"
     CDS             complement(9884..10189)
                     /locus_tag="IW746_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1077584.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10278..11309)
                     /locus_tag="IW746_000662"
     CDS             complement(10278..11309)
                     /locus_tag="IW746_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1077585.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11516..12391
                     /locus_tag="IW746_000663"
     CDS             11516..12391
                     /locus_tag="IW746_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1077586.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12543..13385)
                     /locus_tag="IW746_000664"
     CDS             complement(12543..13385)
                     /locus_tag="IW746_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1077587.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13477..15354)
                     /locus_tag="IW746_000665"
     CDS             complement(13477..15354)
                     /locus_tag="IW746_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1077588.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15351..16766)
                     /locus_tag="IW746_000666"
     CDS             complement(15351..16766)
                     /locus_tag="IW746_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1077589.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETEMVQITLHKLLFPNGQL
                     PKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGASS
                     GMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVVLP
                     VHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVNIL
                     DYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKYAG
                     KKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPDKR
                     LAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLKEI
                     EAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLGVV
                     GMVFASFAIWLFIKKRTGVKK"
     gene            complement(16769..20158)
                     /locus_tag="IW746_000667"
     CDS             complement(16769..20158)
                     /locus_tag="IW746_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1077590.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20415..21807
                     /locus_tag="IW746_000668"
                     /pseudo
     CDS             20415..21807
                     /locus_tag="IW746_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(21880..22527)
                     /locus_tag="IW746_000669"
     CDS             complement(21880..22527)
                     /locus_tag="IW746_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1077591.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22535..23221)
                     /locus_tag="IW746_000670"
     CDS             complement(22535..23221)
                     /locus_tag="IW746_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077592.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23227..24300)
                     /locus_tag="IW746_000671"
     CDS             complement(23227..24300)
                     /locus_tag="IW746_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1077593.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24522..25196
                     /locus_tag="IW746_000672"
     CDS             24522..25196
                     /locus_tag="IW746_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1077594.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25201..26541
                     /locus_tag="IW746_000673"
     CDS             25201..26541
                     /locus_tag="IW746_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1077595.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26690..27676)
                     /locus_tag="IW746_000674"
     CDS             complement(26690..27676)
                     /locus_tag="IW746_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1077596.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28400..29431
                     /locus_tag="IW746_000675"
     CDS             28400..29431
                     /locus_tag="IW746_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1077597.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29540..>29965
                     /locus_tag="IW746_000676"
     CDS             29540..>29965
                     /locus_tag="IW746_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1077598.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8883 a         5616 c         5390 g        10076 t
ORIGIN      
        1 aatatgaacg aattagatat gaatcattgt atttgccgtt gcggaaggat tttttaatta
       61 accacagata aacattgata acgattcgct tattgtaatc aacagattaa atttcatatg
      121 ttcatatgta cggatacatt ttcggaccga ttaattttaa ccattctgtt tatttgctta
      181 cctattagat atcaataaaa aactgcttaa cagctatttc agctgttaag cagttttagg
      241 atttttagtt catattcatt gtttcttata atacagtgtt caatacttct cttaaagcac
      301 ttgttgattt gctgaacatt gctttttctt cttcgttgat tgataattca acaacttcac
      361 gaacaccatt ttcgtcaacg attgatggca cacctgtaaa gatatctttt tcgtcgtatt
      421 caccagttaa ataagcagat acaggcaata cagcttgttc gttattcaag atagctttta
      481 cgatacgtgc agtactcata ccaataccat agtaagttgc ttttttacga tcaatgatct
      541 cataagcagc attacggact ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat
      601 ggtctttttc tacaatttca aacactggtt taccaccgac agttgtatgt gaccatgcag
      661 caacttctga atcaccatgt tcacctaaga tatagccgtg gacgctgcgt gggtcaactt
      721 ttaatttcaa tgcgatttct ttacggaaac gagttgtatc caatgttgtt ccagtaccga
      781 taacacgtga tgttggcaga ccagattcgt tccaagcgat atatgtcaaa atatctactg
      841 ggtttgaagc aacaacgata ataccgtcga atcctgatcc catgatttct tttacgattt
      901 ggcgcatgat tgacgcattt gttttaacta aatctaaacg agattgacca ggtttttggt
      961 tgataccagc tgtgataaca acgatatcag catctttaca ttcttcatag ccacctgacc
     1021 atacagccac attttcatcg ccccaagcca taccatctaa aaggtccaat gcttcccctt
     1081 ctgctttttc ttgattcaca tcgatcaata ctaattcatt agagattcct tggttgatca
     1141 ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac gattactact ttacgacttg
     1201 tttttttcat gaataaacag ctccttttca taactatttg ttactttcat gaaaagtata
     1261 gtcttgttta ttcattttat caacatgtta attataatag ttgttattat aatttttgat
     1321 ggaaacgaat tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc
     1381 tgataataca accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat
     1441 ataataagac agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt
     1501 tcttcttaat gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat
     1561 ttgaataaag aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa
     1621 ctcgtttctt gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga
     1681 aaggaagcat caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa
     1741 tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact
     1801 ctaagcatct cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa
     1861 tgagtcaatt cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg
     1921 tatgtatcaa aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt
     1981 ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa
     2041 aaagaagaaa gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt
     2101 tcttctttta ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga
     2161 gtagcttctt tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta
     2221 ataatttgta aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc
     2281 tcgggaactt ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga
     2341 tcccctcggt ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata
     2401 ttcattcctt gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc
     2461 taattcagct agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag
     2521 ttcacgctgt actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg
     2581 ttgggacatg aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt
     2641 ttgataagcg cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt
     2701 tctctgctgt cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc
     2761 agtatgtcag ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct
     2821 ccctctttta tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga
     2881 caaaaacaaa ctgtgatcat cggattagtg attgcatcga tttgtggtat cctccccctc
     2941 ttctcacaaa gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga
     3001 ttgatcaatt ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc
     3061 tctatgattg gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt
     3121 gcaggacagc tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta
     3181 cctattcttg tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatcg
     3241 catggcacag ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt
     3301 atttttctct tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc
     3361 ctgacgactt attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa
     3421 ttggcaagta tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat
     3481 atgcttacac tttctttact actgatggga atcggctttg tattgattgc cactgtgata
     3541 aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt
     3601 ccttacttat tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact
     3661 tcaatcttat tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt
     3721 ctctcttttg cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg
     3781 ctgttgatta gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg
     3841 ataagagtag atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg
     3901 tgtaaaatga caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag
     3961 atccgccatg gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg
     4021 tactggtaca tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat
     4081 caaccttcca tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa
     4141 aaacatccat gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt
     4201 gtaggacata catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat
     4261 attggctgga cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat
     4321 gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc
     4381 actgaaacgg agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt
     4441 gtagaactaa tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc
     4501 gatgtagaag ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa
     4561 atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg
     4621 gttcaacttc aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc
     4681 gattcttctt tactgaatca gatctggtca gtatcagaag aagccttcaa gttatgtagt
     4741 gatttgtttt ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat
     4801 caatcattca tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata
     4861 ttagcattaa gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg
     4921 atcttgtggt tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta
     4981 cgcatcgtct accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa
     5041 ttagggttta tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat
     5101 aaagaaggaa cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc
     5161 acttgcggag atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc
     5221 tatcaagaaa aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa
     5281 cttggcgctt ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat
     5341 attttcgctg ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat
     5401 gtcttattga atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa
     5461 gatgctttat gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg
     5521 ggagggatgt tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca
     5581 ggaaatgaaa agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg
     5641 ggagcaagcc caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca
     5701 ggttatgaag aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg
     5761 ctacctatca aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact
     5821 tctgatagaa caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa
     5881 cagccacttg tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg
     5941 acatatcaga agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt
     6001 ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag
     6061 ataatacgac aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt
     6121 ctcctagttt cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag
     6181 aagcgatttt aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata
     6241 agctattgaa agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga
     6301 caccagaagc agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt
     6361 gcaagcctat catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa
     6421 ttaatacagc attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat
     6481 cgggtacgag ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc
     6541 gaaccccttg aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa
     6601 agattacagc gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca
     6661 aagcaagtgc gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca
     6721 aaaagctgaa acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa
     6781 actttccgat ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt
     6841 gattgtttcc agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata
     6901 gtgttccttc gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc
     6961 ttactaaaat gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa
     7021 tgaatttata tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga
     7081 tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag
     7141 gaactactcc ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat
     7201 tactgattaa accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc
     7261 cttggatcac ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag
     7321 aaacgaggat ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg
     7381 atagttcctc ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt
     7441 acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt
     7501 agcgtatctt tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt
     7561 ccaacatccg tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct
     7621 tgtttgatgt tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa
     7681 attttcatat cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc
     7741 gtgtaataaa tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc
     7801 ccgaagatgt gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc
     7861 tggattatca agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt
     7921 tacgccatct tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc
     7981 accatactgc tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc
     8041 tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga
     8101 taattggatc atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt
     8161 ttctaaaagt ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc
     8221 ttcttttaat tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga
     8281 aaatcctggt atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc
     8341 agtcatgatt ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac
     8401 agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt
     8461 agtctctgta aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga
     8521 agagctaatg ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc
     8581 gtccgctttt ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg
     8641 gatgctcttc cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat
     8701 ggaagcatat tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga
     8761 aatacgaaaa taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga
     8821 agcaaattct acggccatca ggaattccat gcgatggatg acttctgaag aacattccag
     8881 caaaaaatca agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga
     8941 gaaaaatata atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg
     9001 actaatgtca gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga
     9061 tgtttttcca attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt
     9121 tggtgcgaat cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc
     9181 gtaataatca atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga
     9241 ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag
     9301 cagcctaaaa ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt
     9361 tcttatcttt ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa
     9421 atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg
     9481 ctcttgttcg gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc
     9541 gattgctcgg aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt
     9601 taattgtttg atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact
     9661 gatttctctt ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc
     9721 ctacttatat tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag
     9781 ttggattttt tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca
     9841 ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt
     9901 tattttttga ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat
     9961 ctttttcaaa gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag
    10021 ataaagctgt ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt
    10081 tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac ttcttcttct gttttagggg
    10141 ccattgaacc tcttaatcgt ttgtgttttt caccgtcata atttgtcatg ttggttcctc
    10201 ctagtagttc tatacttttt catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc
    10261 cgattcttac aaatatgtca tatttttcct tctgattctt gaatgctttt gattccttct
    10321 tccaaaattt tcatttgtaa gattgtttct tcatctttga tgactttttc tgcgccatta
    10381 actaatgtct cgtaaatccc ttcgtagaca cggctgtaat caccaacctc ggaaattact
    10441 ttttcttcat gataactccc ttgttcatct atataggtca atacaccata atgttcgggc
    10501 aaatcgatac caaagtcttc atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt
    10561 cgatcttttg tttgtttcac gaacatccct ttttttccgt aaacgacgaa acttggacgt
    10621 tctttgatgc ggaaataact ggattttatc gaaattttca tatttccata gaaaaaatcc
    10681 agatcgaagt aatcattcat tcgtcctttc cctaaaagtt gacgtacatc atagctgata
    10741 tcatctggtt cgccgaaata agaaagaact tgatctactg tatgacaagc atgtccatat
    10801 aaatagctgt tcgcagtaga aaattcatgc gtattttctg gtacttccgg tctgaaataa
    10861 tcataatgca tttctacttc taaaatatcc cctaattttc cgctttcgat tactttttgg
    10921 actgtcaaga agtcagaatc gaaccgtctg ttttggtaac attggataaa tagacctttt
    10981 tcttttgcta atgcaaataa ctcttctgct tcctttgacg tttctgcaaa aggtttctct
    11041 actagtacat tcttaccatg ttcgagtgcc atttttccat attcataatg aaattgacta
    11101 ggtgtagtga tgacaactaa ttggatctca ggatcatccc aaagatcaga cagctgatca
    11161 gtatattcga tgtcttcgaa tgttttccac actggttcag atggggaaaa aatttttttg
    11221 atattgattt tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt
    11281 ccattgccaa gataagcgat tgtaagcatg agtggtgcct ccttttattg atgttttcag
    11341 tatatcctag aaaagcgcag aaagtattta taatagctat tctggtaaaa aagttcagaa
    11401 aaacacttgt tatcattcgg tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa
    11461 aagatagtag cggaaccaag tgtttatcag taagatagaa tttggaggtc agatcatgct
    11521 tattgaagaa aaactaacaa aacaagagtt gtttaccacg acagagaaac ggattgtcga
    11581 ttacatccgt aggaatatcg aagctgcagt ctacatgacg attgaagaat tagcaaaagc
    11641 tacgtatact tcccattcag ctatcattcg tttatgcaaa aaaatgggat tcagcggatt
    11701 taaagaattc cgtttcgagc tggggcggga agttcatcaa ttgattgctc agttcaatca
    11761 aacgattgat cctaattttc ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa
    11821 aatggcggat ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg aacatgaaga
    11881 tttagatcaa atcggtaaag tattgaacaa ggcacagcga atctttcttt ttgcaaaagg
    11941 tgattctcaa atcacggcaa ggaaattcca gaataaaatg gtcaagttga ataagttttt
    12001 gatcatggct gaagaataca gtgattcttc ttggaatgca gccaatttga ctagcgagga
    12061 ttgcgccatt tttattagct acagcggtcg catccatcat tatgaacgaa taatgaccta
    12121 tttgaagcat gtagaggccc ctaccttgct gattaccgga aatcagcatt cagaaatggc
    12181 caaacaggct gatatgtgtc ttgttatatc gcaggaggaa tatgattttg ctaaagtagc
    12241 gactttctcc tctcaaattg ccttcgatta cgtattaaat acgttattct cagtgatata
    12301 ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa
    12361 aggccttctt aaagaatatc ggaaaactta agaaaagatc ctcatttagt aataagaaag
    12421 atagtgtttg ataggctctc tttgatatca tcataaaaac aggctgaagt tttctttttc
    12481 aaacctcagc ctgtttgttt acggaacgat tcgaatagat atattataga aatgaggaat
    12541 agctaacttc tgtttcttat tttgaacgat tgacgaaaaa gatatataag aaggaggaaa
    12601 atcagtatac atgcagctaa caatccatac aggaaaaatc gtttgttctc ggatactttc
    12661 tgtacttctt tttgaacttc ttttgcatga taaggaaccc ggtgccctct gacaagtaag
    12721 cgatgactgt tgaccatata aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt
    12781 tcgattctca gatcttttgt atcagtcggt tctaccgtct tgatctgatc gacttgataa
    12841 gcgagtgtcc gtccattgat ttcgatatag aaagaatcac ctaaagtcag tttcggtaag
    12901 tctgtgaaca actttgcttg aggcagtcct cgatggctgg aaataacggc atgtgtattt
    12961 tctccccccg taggataaga cgttccttcc aataatgagg cacctttttc tagcagcaca
    13021 gtattcgttt catcaaaaat tggcaaatgg acattgatct cagggatcgt caaaataccg
    13081 atcgtatgtt tctcaaaata ggttctatcc tttttcgcag gttctttttt cttagtaaag
    13141 ggatctgcac ctggatttcc accttctttt gctaattgct ggtttttctt tgtcatgttt
    13201 tcttggattt tagccatgac ttcttcattt tctttgaccg cttgttgctg ataatgagag
    13261 atgatctgct gatccagata attattcagc gcgtcgctaa caaatggata agctagtatc
    13321 cctattcctg agagaagcaa gaaaagcatc agcagatcta gaagtttcct tttctttggt
    13381 ttcatttgtg ttctccttgt gatgactgtt tgataataaa agaaaaagga gcatttgcgt
    13441 aatccgctgc tcctttttcc aattcttgca aaaagactaa atctgactgt gcttgcgtct
    13501 agcaaagtac agtccagcaa tcagtagaag gactactcct gcaccgatat acacatagat
    13561 tcccttaccg cctgttgaag gaagtgtacc tttgtgttta tttggtattt tttcaggaga
    13621 aatcaactgt cctgctgtta cataagattg ttcatcgata gtaaatgcta cacggtttgt
    13681 taatggaaca tatttttctg gcgctttcgt ttcttccaga tagtacgtgc catattttag
    13741 acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa accgttgctg attctttcgc
    13801 cgatacccag ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact
    13861 atcttgatca cgaacgacga attctgctcc agcaagtgtt tggtctgatg taacgtcacc
    13921 atctacttta acaaatcgtt tgcccccagt aacgacatcg actgacggtg gtgtttgatc
    13981 atttgtatgc ccgttatcga catttgcttg gttagaaaat cctttggttg gatctgcttt
    14041 ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg ccaggagtta atgaaggaat
    14101 ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag aataatttac
    14161 tgggtcgatt tctttatttc catcatataa ggcataagca tacgttcctg aagaatcatt
    14221 atcaaatgtt aaagcagcgt catgagtatc gataagtttg aatgttgtgt acttgttttg
    14281 cgttccttct ttatcagcga tacctaatgg gatattgaca gaaatctcat attgaatttt
    14341 ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt
    14401 atcatttttg atggtctttt ctactggtgt ttggctattt gccaggatct caaaaggcgt
    14461 gattgtttgc ttttcaatca tttccgcatt atcaggagct tttacttctt ctaaattata
    14521 ttttccaact tctaaatgat taacgatcaa ctggcctttt tcaatagatg cctcggcaaa
    14581 gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt tggtttgatc
    14641 agttgtccaa gtgtacaatc catctgtgac actttggatg tattttttga cgcttggtgt
    14701 tccttcttct ttagaaataa taaattctgc tccatttagt gcttcgtttt cggcagtacc
    14761 gatttttgta actttcaacg ttccatcatt accgactgta tttttagggt agagatggat
    14821 agtatctagt tcttctgtcc cgtatttata agagccatct gcttgtttga tcatctcata
    14881 tacagggaaa gctaaaacca tgtttgcggc agctgacact ccgtcttttg gttcttcttt
    14941 gatcgtgtag actgcatctt tcccattttg ttttttaggt aaagataaag tgacatttcc
    15001 atcagcatct gtcgttcctg aagcaactgg tgtaccagga gtcaaagact ggactgcttg
    15061 ttttgctgca tccacggacg ctcctttatc tcgttgcgca taaaattctt gagtgacgtt
    15121 ataaactgaa aatgaaatat cggctaatcc ttggtattga tcgaattcgc tcatttcttt
    15181 cccgctgttt tggattaaag gatcgggtaa atcagtcatt ttctttttat gaaggatgac
    15241 ttgagctgct gtctcctctg caaacacttt ttttgcgccg aagctgtttg tgagtaatgg
    15301 taaaataagg aaaaggactc ctaacataac gtttattttt ttatgatttt tcattttttc
    15361 actcctgttc tttttttgat aaacaaccag attgcaaagc ttgcgaatac cataccaaca
    15421 acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa gaaatggacg gctattttct
    15481 tttttattga ctactttcaa tatactgtcc gcctctttat cctcagaaag aaaagttgaa
    15541 atggtaaacg gaatttctgt ttcacttaaa atataaccag aaggtgcttc gatctctttt
    15601 aatcgataac ttcctgtttt caacccggaa attgaaaaag cgccattttt atcggaaatc
    15661 aggtgaagcg catctgattc gttctttgtc cacttgtatc cgttggctgt ttgctggagg
    15721 taatttcctg ctttgttttt aataaggaat actgcgccag ctaatcgttt atctggttta
    15781 tttgtctcga ttttgacaaa ttgcttactc cctgtatata cagagacttt ctttgtactg
    15841 acaccaaaac caaaatccag tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta
    15901 ctgcttagct gcatctgata agaaatggtc agtttttttc cagcatattt ttccaacgaa
    15961 gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc
    16021 agcttttctc cattcgatga aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc
    16081 gcactatctg acaattcgaa cttttgatag tcaaggatat ttacaggaat tgtcgtatgc
    16141 aaggaataag tgattttttc accaatcgta aaatcatttc taggctcttc taataccttc
    16201 tcaaaaggtg gatcagggta gtcgttttct tcatttttag gatacaaatg gatagttgaa
    16261 agtttttgat tgttttgtcc atgtacagga agaacaacta ccatattctc tgccttttct
    16321 ttgacgactt ctggtacttt ggattcaatg aaaagatacg ctttatctct tttttcttga
    16381 tctttagcgg ccagagaaaa agaagcgata ccatcttcgt tgttaagagt tgtagttgtt
    16441 gcttctgcgg taaacatacc ggaagacgca ccgttttttg cgatctctgc ttgtgcttct
    16501 tctaccgttt tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg
    16561 aatgtgacac catttaatcc tcgatacgtt tgtaataaag ctttttcttg tccgtcattt
    16621 ggatgatttt tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc
    16681 atttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16741 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16801 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16861 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16921 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16981 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    17041 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    17101 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    17161 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    17221 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17281 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17341 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17401 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17461 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17521 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17581 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17641 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17701 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17761 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17821 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17881 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17941 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    18001 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    18061 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    18121 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    18181 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18241 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18301 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18361 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18421 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18481 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18541 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18601 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18661 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18721 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18781 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18841 gagaagtatt tcccggccga tcctgcgtat tagaaaactg agttccgtaa taattgctgc
    18901 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18961 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    19021 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    19081 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    19141 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    19201 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19261 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19321 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19381 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19441 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19501 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19561 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19621 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19681 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19741 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19801 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19861 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19921 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19981 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    20041 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    20101 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    20161 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    20221 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20281 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20341 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaac
    20401 gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20461 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtataatc
    20521 gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg
    20581 tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa
    20641 agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag
    20701 tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc
    20761 aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact
    20821 agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac
    20881 ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag
    20941 aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt
    21001 atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct
    21061 ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat
    21121 ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg
    21181 attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat
    21241 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
    21301 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
    21361 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
    21421 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
    21481 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
    21541 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
    21601 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
    21661 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
    21721 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
    21781 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
    21841 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
    21901 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
    21961 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
    22021 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
    22081 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
    22141 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
    22201 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
    22261 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
    22321 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
    22381 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
    22441 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
    22501 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
    22561 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
    22621 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
    22681 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
    22741 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
    22801 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
    22861 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
    22921 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
    22981 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
    23041 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
    23101 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
    23161 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
    23221 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
    23281 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
    23341 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
    23401 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
    23461 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
    23521 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
    23581 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
    23641 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
    23701 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
    23761 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
    23821 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
    23881 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
    23941 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
    24001 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
    24061 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
    24121 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
    24181 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
    24241 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
    24301 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
    24361 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
    24421 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
    24481 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
    24541 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
    24601 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
    24661 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
    24721 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
    24781 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
    24841 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
    24901 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
    24961 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
    25021 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
    25081 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
    25141 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
    25201 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
    25261 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
    25321 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
    25381 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
    25441 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
    25501 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
    25561 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
    25621 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
    25681 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
    25741 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
    25801 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
    25861 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
    25921 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
    25981 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
    26041 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
    26101 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
    26161 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
    26221 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
    26281 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
    26341 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
    26401 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
    26461 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
    26521 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
    26581 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
    26641 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
    26701 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
    26761 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
    26821 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
    26881 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
    26941 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
    27001 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
    27061 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
    27121 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
    27181 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
    27241 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
    27301 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
    27361 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
    27421 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
    27481 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
    27541 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
    27601 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
    27661 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
    27721 cttataaatt tattctatag gagacgattg ttttctacaa atatttagat tggaatttga
    27781 ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat attctaataa
    27841 aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc cactcgttgt
    27901 gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa aggattaatt
    27961 ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga agtaccaact
    28021 atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc attattgaat
    28081 aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag
    28141 agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta accacatagc
    28201 taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata aaataactaa
    28261 aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt tgcttcctaa
    28321 gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag aaaatgaaca
    28381 ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag aaaagttaaa
    28441 gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa atcgttcttc
    28501 cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta ctgattattt
    28561 gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag aaaaacaaga
    28621 ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa ctacagcgaa
    28681 ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga agcaatattc
    28741 attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag agatttctct
    28801 tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg atgcagaact
    28861 tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa gcagagaagc
    28921 tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc ttctgtattg
    28981 cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt tgaccaagca
    29041 gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac caaaagaagc
    29101 gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg aaaattcttt
    29161 tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc aaattttgag
    29221 caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat atcgttatta
    29281 tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta atcgatacct
    29341 ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt atcacgaatt
    29401 agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt tttcacaata
    29461 aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat attacttaac
    29521 aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga acttatttac
    29581 gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg atgagccact
    29641 ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt
    29701 gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag ttattattta
    29761 taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc ttggtgcagt
    29821 gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta tcgatcgttt
    29881 agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa
    29941 tggaacaaaa tcaaaacaga ttcct
//
LOCUS       DACEND010000017        29759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEND010000017 DACEND010000000
VERSION     DACEND010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.018"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW746_000677"
     CDS             147..1016
                     /locus_tag="IW746_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1077599.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW746_000678"
     CDS             1225..2250
                     /locus_tag="IW746_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1077600.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW746_000679"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW746_000679"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1077601.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IW746_000680"
     CDS             3333..5036
                     /locus_tag="IW746_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1077602.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGEIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW746_000681"
     CDS             5038..7071
                     /locus_tag="IW746_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1077603.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW746_000682"
     CDS             7082..7522
                     /locus_tag="IW746_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1077604.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW746_000683"
     CDS             complement(7722..8849)
                     /locus_tag="IW746_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077605.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW746_000684"
     CDS             complement(8852..9529)
                     /locus_tag="IW746_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077606.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW746_000685"
     CDS             9793..11559
                     /locus_tag="IW746_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1077607.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW746_000686"
     CDS             11574..12011
                     /locus_tag="IW746_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1077608.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW746_000687"
     CDS             12024..13175
                     /locus_tag="IW746_000687"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1077609.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IW746_000688"
     CDS             complement(13237..13935)
                     /locus_tag="IW746_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1077610.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IW746_000689"
     CDS             complement(13928..15280)
                     /locus_tag="IW746_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1077611.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IW746_000690"
     CDS             complement(15503..16903)
                     /locus_tag="IW746_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1077612.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IW746_000691"
     CDS             17064..17801
                     /locus_tag="IW746_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1077613.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IW746_000692"
     CDS             17926..18405
                     /locus_tag="IW746_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1077614.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IW746_000693"
     CDS             18402..19169
                     /locus_tag="IW746_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077615.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IW746_000694"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IW746_000694"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1077616.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IW746_000695"
     CDS             complement(20072..20674)
                     /locus_tag="IW746_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1077617.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IW746_000696"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IW746_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IW746_000697"
     CDS             21355..21939
                     /locus_tag="IW746_000697"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1077618.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IW746_000698"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IW746_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1077619.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IW746_000699"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IW746_000699"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1077620.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IW746_000700"
     CDS             23840..24019
                     /locus_tag="IW746_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077621.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IW746_000701"
     CDS             24041..25066
                     /locus_tag="IW746_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1077622.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IW746_000702"
     CDS             25241..26485
                     /locus_tag="IW746_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1077623.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IW746_000703"
     CDS             26638..27585
                     /locus_tag="IW746_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1077624.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IW746_000704"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IW746_000704"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1077625.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IW746_000705"
     CDS             complement(28487..28729)
                     /locus_tag="IW746_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077626.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IW746_000706"
     tRNA            28850..28922
                     /locus_tag="IW746_000706"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IW746_000707"
     CDS             complement(29041..29679)
                     /locus_tag="IW746_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1077627.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10127 a         5246 c         6060 g         8326 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacctaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgaaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgg
//
LOCUS       DACEND010000018        29581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEND010000018 DACEND010000000
VERSION     DACEND010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.019"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW746_000708"
     CDS             complement(105..1562)
                     /gene="ascB"
                     /locus_tag="IW746_000708"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1077628.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1559..3454)
                     /locus_tag="IW746_000709"
     CDS             complement(1559..3454)
                     /locus_tag="IW746_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1077629.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3512..5287)
                     /locus_tag="IW746_000710"
     CDS             complement(3512..5287)
                     /locus_tag="IW746_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1077630.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5337..6833)
                     /locus_tag="IW746_000711"
     CDS             complement(5337..6833)
                     /locus_tag="IW746_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1077631.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7027..8649)
                     /locus_tag="IW746_000712"
     CDS             complement(7027..8649)
                     /locus_tag="IW746_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1077632.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8977..10290)
                     /locus_tag="IW746_000713"
     CDS             complement(8977..10290)
                     /locus_tag="IW746_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1077633.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10535..10759)
                     /locus_tag="IW746_000714"
     CDS             complement(10535..10759)
                     /locus_tag="IW746_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077634.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10779..11147)
                     /locus_tag="IW746_000715"
     CDS             complement(10779..11147)
                     /locus_tag="IW746_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1077635.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11344..12054)
                     /locus_tag="IW746_000716"
     CDS             complement(11344..12054)
                     /locus_tag="IW746_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077636.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12072..12569)
                     /locus_tag="IW746_000717"
     CDS             complement(12072..12569)
                     /locus_tag="IW746_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077637.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12606..13058)
                     /locus_tag="IW746_000718"
     CDS             complement(12606..13058)
                     /locus_tag="IW746_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1077638.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13193..13798)
                     /locus_tag="IW746_000719"
     CDS             complement(13193..13798)
                     /locus_tag="IW746_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1077639.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(14248..14868)
                     /locus_tag="IW746_000720"
     CDS             complement(14248..14868)
                     /locus_tag="IW746_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077640.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(14870..15739)
                     /locus_tag="IW746_000721"
     CDS             complement(14870..15739)
                     /locus_tag="IW746_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1077641.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(15762..16169)
                     /locus_tag="IW746_000722"
     CDS             complement(15762..16169)
                     /locus_tag="IW746_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1077642.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(16188..17477)
                     /locus_tag="IW746_000723"
     CDS             complement(16188..17477)
                     /locus_tag="IW746_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1077643.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(17504..17806)
                     /locus_tag="IW746_000724"
     CDS             complement(17504..17806)
                     /locus_tag="IW746_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1077644.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(17831..19924)
                     /locus_tag="IW746_000725"
     CDS             complement(17831..19924)
                     /locus_tag="IW746_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1077645.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(20413..21177)
                     /locus_tag="IW746_000726"
     CDS             complement(20413..21177)
                     /locus_tag="IW746_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1077646.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(21288..22157)
                     /gene="mdcB"
                     /locus_tag="IW746_000727"
     CDS             complement(21288..22157)
                     /gene="mdcB"
                     /locus_tag="IW746_000727"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1077647.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(22182..23258)
                     /locus_tag="IW746_000728"
     CDS             complement(22182..23258)
                     /locus_tag="IW746_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1077648.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(23282..23875)
                     /locus_tag="IW746_000729"
     CDS             complement(23282..23875)
                     /locus_tag="IW746_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1077649.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(23879..25555)
                     /gene="mdcD"
                     /locus_tag="IW746_000730"
     CDS             complement(23879..25555)
                     /gene="mdcD"
                     /locus_tag="IW746_000730"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1077650.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(25545..25844)
                     /locus_tag="IW746_000731"
     CDS             complement(25545..25844)
                     /locus_tag="IW746_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1077651.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(25946..27592)
                     /gene="mdcA"
                     /locus_tag="IW746_000732"
     CDS             complement(25946..27592)
                     /gene="mdcA"
                     /locus_tag="IW746_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1077652.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(27599..28510)
                     /locus_tag="IW746_000733"
     CDS             complement(27599..28510)
                     /locus_tag="IW746_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1077653.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            28630..29487
                     /locus_tag="IW746_000734"
     CDS             28630..29487
                     /locus_tag="IW746_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1077654.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         8494 a         6279 c         4993 g         9815 t
ORIGIN      
        1 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
       61 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      121 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      181 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      241 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      301 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      361 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      421 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      481 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      541 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      601 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      661 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      721 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      781 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      841 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
      901 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
      961 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1021 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1081 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1141 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1201 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1261 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1321 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1381 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1441 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1501 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1561 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1621 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1681 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1741 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1801 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1861 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     1921 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     1981 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2041 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2101 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2161 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2221 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2281 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2341 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2401 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2461 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2521 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2581 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2641 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2701 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2761 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2821 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     2881 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     2941 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3001 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3061 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3121 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3181 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3241 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3301 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3361 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3421 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3481 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3541 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3601 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3661 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3721 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3781 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3841 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     3901 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     3961 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4021 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4081 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4141 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4201 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4261 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4321 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4381 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4441 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4501 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4561 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4621 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4681 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt
     4741 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4801 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4861 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     4921 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     4981 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5041 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5101 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5161 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5221 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5281 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5341 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5401 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5461 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5521 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5581 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5641 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5701 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5761 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5821 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     5881 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     5941 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6001 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6061 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6121 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6181 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6241 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6301 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6361 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6421 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6481 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6541 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6601 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6661 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6721 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6781 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6841 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     6901 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     6961 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7021 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7081 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7141 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7201 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7261 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7321 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7381 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7441 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7501 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7561 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7621 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7681 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7741 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7801 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7861 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     7921 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     7981 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8041 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8101 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8161 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8221 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8281 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8341 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8401 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8461 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8521 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8581 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8641 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8701 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8761 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8821 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     8881 cccttggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
     8941 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
     9001 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
     9061 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
     9121 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
     9181 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
     9241 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
     9301 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
     9361 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
     9421 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
     9481 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
     9541 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
     9601 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
     9661 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
     9721 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
     9781 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
     9841 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
     9901 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
     9961 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
    10021 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
    10081 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
    10141 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
    10201 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
    10261 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
    10321 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
    10381 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
    10441 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
    10501 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
    10561 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
    10621 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
    10681 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
    10741 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
    10801 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
    10861 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
    10921 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
    10981 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
    11041 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
    11101 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
    11161 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
    11221 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
    11281 ccacaaactt tttttacaaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt
    11341 cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc
    11401 aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg
    11461 ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt
    11521 tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat
    11581 gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc
    11641 accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca
    11701 aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa
    11761 aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt
    11821 tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa
    11881 tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata
    11941 cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat
    12001 ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc
    12061 acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc
    12121 atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca
    12181 acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt
    12241 tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca
    12301 tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta
    12361 tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct
    12421 ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct
    12481 aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt
    12541 tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct
    12601 tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc
    12661 ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt
    12721 aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct
    12781 tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc
    12841 atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc
    12901 atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga
    12961 tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct
    13021 cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt
    13081 tgtaagacta gttcacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag
    13141 ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac
    13201 aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg
    13261 atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa
    13321 taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa
    13381 gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta
    13441 ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta
    13501 tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga
    13561 ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc
    13621 caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt
    13681 ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat
    13741 atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc
    13801 tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa
    13861 ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga
    13921 acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag cttgcatctg
    13981 cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg ctgttcttat
    14041 taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct cctaccaact
    14101 cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg aaattgctcc
    14161 ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt tcaaaagcca
    14221 atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt tttctaattt
    14281 atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt catatagtgt
    14341 tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg cttctttcgt
    14401 aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat aatgatccat
    14461 gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt gccataacac
    14521 ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat ctgctaattc
    14581 ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca ctttctttcc
    14641 tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg tcctttcttg
    14701 ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt ccttgatttc
    14761 atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca taagatatgt
    14821 ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct catttatttc
    14881 ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg acaatttctt
    14941 ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt tctgtttcac
    15001 taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca acattgaatt
    15061 tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct gttcctccat
    15121 ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga atgtttactt
    15181 ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat gcatccacgc
    15241 ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg tcattttcag
    15301 cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct acggaaacac
    15361 cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct ttgaaaggca
    15421 gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct ttgatatcat
    15481 caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct tctgcaatag
    15541 ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg acacttgttt
    15601 gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga agcatttcta
    15661 ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt tttgttactt
    15721 ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt tcctttgatc
    15781 gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta atcgatccct
    15841 tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct catcagtttt
    15901 ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata ttggatggct
    15961 tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag tttcgctaat
    16021 ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc
    16081 ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt tttcggttct
    16141 ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta aaatgctttc
    16201 aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc tacatcggtt
    16261 gctgtagcat tgatgaatcc catcgaatta aagatcgtta ctagcaaagc gggcaataat
    16321 gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc gccaactgcg
    16381 ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc aggtaaaatc
    16441 atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat cgttgtcgta
    16501 atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca atctaaggca
    16561 gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac gatttcacct
    16621 aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg gatggcttgt
    16681 aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa tggttcgcct
    16741 gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga caaataagta
    16801 tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt tttattttcg
    16861 cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat cgtacaaaaa
    16921 tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg cgctactgcc
    16981 ggcatagcaa tggcgaaaca agctccgata aaatcaccga ccaagatcaa tacgatccct
    17041 cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa aattgcttga
    17101 ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt gaatacaaaa
    17161 atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat tgcttgacca
    17221 ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt aaagattttc
    17281 ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac ttgaaatcct
    17341 aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg cagtagtaga
    17401 cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt ttcgatgata
    17461 aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata actcccttat
    17521 cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata tttttcaaat
    17581 agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga aaattttctg
    17641 ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt
    17701 tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct gcaaaactac
    17761 ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt gttctccccc
    17821 gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct tcgatatttg
    17881 tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt agttctttca
    17941 tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc tttactggat
    18001 ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg actcctaatt
    18061 tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca atcacgatat
    18121 acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct tcgacaatgt
    18181 tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta gtggcttttt
    18241 ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg atctcccttt
    18301 tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag atgattcgat
    18361 ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac tccgtcgatt
    18421 catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag tcttctataa
    18481 ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta tttcgataat
    18541 tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat tcgatttctt
    18601 ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga ttctctgcta
    18661 acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa acataattca
    18721 agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt
    18781 cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga gtgactgcgc
    18841 ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg acttggatat
    18901 gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg aataaagctt
    18961 cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc aattggatcg
    19021 ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca ttgaacgtct
    19081 caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat tgaaattcct
    19141 cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta tttttccaat
    19201 taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat gtaaaaagta
    19261 ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa tagatctcct
    19321 gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga gccggtgtta
    19381 attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg ataaaatcat
    19441 agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc tttccataac
    19501 tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta
    19561 atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc gggattcggc
    19621 tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt
    19681 tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc ttcaacaaaa
    19741 aacctttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac cagatattcg
    19801 cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt gctagatgtt
    19861 ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt gttcttgttt
    19921 tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata ataaaaagta
    19981 gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa ataagccata
    20041 tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa ttttgcccat
    20101 ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga acaactaaac
    20161 taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg caagctttta
    20221 aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat aagaagcgat
    20281 tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata ggctatctct
    20341 ggcacaccgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa ctccgttcat
    20401 aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc atcttttttc
    20461 tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg tcttacttcc
    20521 tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga aatcaattcg
    20581 gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa tagttatttt
    20641 tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct gatgttcggc
    20701 aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt cccaagcgga
    20761 ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt ctttagccgt
    20821 tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt tattcttgac
    20881 gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa atcccgtttg
    20941 ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc tgccgtaaga
    21001 acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc tgcccgaact
    21061 tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt gggtcggcag
    21121 agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt ttttcattgt
    21181 cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct aaaaggaaga
    21241 gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta gtcattgagt
    21301 aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag catgaacaag
    21361 cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg acaaaagtgc
    21421 aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg agcaagtttt
    21481 tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa
    21541 ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt atctagctcc
    21601 ttagctgctt gaattgcaag cctgatatgc ggatacccta atatcgcttc ttcatatgca
    21661 ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt atgacgatat
    21721 tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga taaagaaaaa
    21781 ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat tgcccaaatc
    21841 gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc cgcttttcgc
    21901 ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca tgctatttga
    21961 gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa agcatagtcc
    22021 atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg acttaatgtt
    22081 acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac tgcttttttt
    22141 cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct gaaacctttt
    22201 ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca acttgccgtc
    22261 attagataag ctacaagaat catgactcct gaaggtaact gcagccaact atctttaaac
    22321 aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata agcataaaca
    22381 gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt ggaagcgcct
    22441 atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt caagtaaggt
    22501 gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga aaagaacgcg
    22561 ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg atagactttt
    22621 tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa ccgatctaat
    22681 aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa cagataccat
    22741 gcgacatata aaatcgttga accaatcaat actggccata catatttttt attagcaaat
    22801 actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag catcataacg
    22861 gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa gcttgcagta
    22921 tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat
    22981 agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa
    23041 tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat
    23101 ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc
    23161 aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac tttctggaag
    23221 tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt
    23281 attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga atttttttct
    23341 ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat
    23401 cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg
    23461 aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg
    23521 ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag
    23581 cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt
    23641 gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg
    23701 accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg
    23761 tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg
    23821 tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt
    23881 atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg
    23941 ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat
    24001 cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt
    24061 ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat
    24121 cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg
    24181 tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag
    24241 caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg
    24301 caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca gcagcactat
    24361 tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct tggcttggta
    24421 catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct tcgtaaacgt
    24481 gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac
    24541 ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct tctatgaatt
    24601 tacttgattt accataatac acagtactaa tagaagaagt tggatttttc atgccagtta
    24661 gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt
    24721 ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg
    24781 ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac
    24841 tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta
    24901 cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga gatcctaatg
    24961 tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa
    25021 taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta
    25081 atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga
    25141 ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc
    25201 cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct
    25261 catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg
    25321 aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt
    25381 tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt
    25441 gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt
    25501 caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa
    25561 agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca
    25621 ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc
    25681 catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct
    25741 gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta
    25801 ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt
    25861 tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg
    25921 cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat
    25981 acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat aatgaacgtt
    26041 tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg gctgcacgca
    26101 agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg
    26161 caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca attccttctt
    26221 ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg gctaagcctg
    26281 cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt ttatttgaac
    26341 catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga tcatcattgt
    26401 cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt
    26461 taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag tttccttcgt
    26521 aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct gcagcttgtg
    26581 ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag
    26641 cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt tcaacccaac
    26701 ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt
    26761 tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata gctgctgtat
    26821 tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt cctttgattg
    26881 ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag
    26941 cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca ccagggatat
    27001 ctacacgtgg taaagaatcg acaacttcgt tgacttgaac gatcacgata ccatctttga
    27061 aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg tacaagttgc
    27121 cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa tcgatgaata
    27181 agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa
    27241 tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga
    27301 ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca
    27361 gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt
    27421 tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt
    27481 ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc
    27541 gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc
    27601 atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg
    27661 tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg
    27721 ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc
    27781 actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct gcgtcctgag
    27841 tggctcttcc atccgtattt tttatataca aacatctagc atcagataga tggacagact
    27901 gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag
    27961 ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg
    28021 ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat
    28081 aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg cttctcgtca
    28141 cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt aagactgcgc
    28201 ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca acagctgcac
    28261 aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt ttttccattt
    28321 catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat
    28381 agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca
    28441 tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac
    28501 ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt
    28561 tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga
    28621 tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat
    28681 gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa
    28741 catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg
    28801 accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac attagaagaa
    28861 ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat attgtattta
    28921 gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa gcatcctgat
    28981 atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc tgtactgtac
    29041 atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga acctgtttat
    29101 gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt agaagatatc
    29161 caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac actggatgca
    29221 gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga tcctgcgaca
    29281 ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt ttcttggtct
    29341 tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca
    29401 atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat tgcaaaaacg
    29461 atgcgggaat tttatttagg aaaataaaaa aaaaagagct gggacaaaat tcttgtcgca
    29521 gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa gccgaaattt
    29581 c
//
LOCUS       DACEND010000019        29104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEND010000019 DACEND010000000
VERSION     DACEND010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.020"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW746_000735"
     CDS             complement(<1..1031)
                     /locus_tag="IW746_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1077655.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IW746_000736"
     CDS             complement(1113..1802)
                     /locus_tag="IW746_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1077656.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW746_000737"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW746_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1077657.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IW746_000738"
     CDS             complement(2329..2832)
                     /locus_tag="IW746_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1077658.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW746_000739"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW746_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1077659.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW746_000740"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW746_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1077660.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW746_000741"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW746_000741"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1077661.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW746_000742"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW746_000742"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1077662.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IW746_000743"
     CDS             complement(6444..7463)
                     /locus_tag="IW746_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1077663.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IW746_000744"
     CDS             complement(7636..8523)
                     /locus_tag="IW746_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1077664.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW746_000745"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW746_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1077665.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW746_000746"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW746_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1077666.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW746_000747"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW746_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1077667.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IW746_000748"
     CDS             9754..10398
                     /locus_tag="IW746_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1077668.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IW746_000749"
     CDS             complement(10454..10957)
                     /locus_tag="IW746_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1077669.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IW746_000750"
     CDS             11554..12519
                     /locus_tag="IW746_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1077670.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IW746_000751"
     CDS             complement(12618..13790)
                     /locus_tag="IW746_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077671.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IW746_000752"
     CDS             complement(13777..14922)
                     /locus_tag="IW746_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077672.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IW746_000753"
     CDS             complement(14936..15868)
                     /locus_tag="IW746_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077673.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IW746_000754"
     CDS             15985..17064
                     /locus_tag="IW746_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1077674.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IW746_000755"
     CDS             17061..17690
                     /locus_tag="IW746_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1077675.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IW746_000756"
     CDS             complement(17800..18027)
                     /locus_tag="IW746_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1077676.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IW746_000757"
     CDS             complement(18130..20718)
                     /locus_tag="IW746_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1077677.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IW746_000758"
     CDS             complement(20738..21397)
                     /locus_tag="IW746_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1077678.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW746_000759"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW746_000759"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1077679.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IW746_000760"
     CDS             complement(22170..23036)
                     /locus_tag="IW746_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1077680.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IW746_000761"
     CDS             23145..23786
                     /locus_tag="IW746_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1077681.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW746_000762"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW746_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1077682.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IW746_000763"
     CDS             complement(25227..26126)
                     /locus_tag="IW746_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1077683.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IW746_000764"
     CDS             complement(26128..26925)
                     /locus_tag="IW746_000764"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1077684.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IW746_000765"
     CDS             complement(26903..27607)
                     /locus_tag="IW746_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1077685.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IW746_000766"
     CDS             27738..28313
                     /locus_tag="IW746_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1077686.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..>29104
                     /locus_tag="IW746_000767"
     CDS             28431..>29104
                     /locus_tag="IW746_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1077687.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8562 a         6169 c         5232 g         9141 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaa
//
LOCUS       DACEND010000020        28546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEND010000020 DACEND010000000
VERSION     DACEND010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.021"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..715
                     /gene="purE"
                     /locus_tag="IW746_000768"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="IW746_000768"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1077688.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            699..1832
                     /gene="purK"
                     /locus_tag="IW746_000769"
     CDS             699..1832
                     /gene="purK"
                     /locus_tag="IW746_000769"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1077689.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="IW746_000770"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="IW746_000770"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1077690.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="IW746_000771"
     CDS             complement(3320..3697)
                     /locus_tag="IW746_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077691.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /locus_tag="IW746_000772"
     CDS             3953..4681
                     /locus_tag="IW746_000772"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1077692.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="IW746_000773"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="IW746_000773"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1077693.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="IW746_000774"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="IW746_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1077694.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="IW746_000775"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="IW746_000775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1077695.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /locus_tag="IW746_000776"
     CDS             7816..9255
                     /locus_tag="IW746_000776"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1077696.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9287..10330
                     /locus_tag="IW746_000777"
     CDS             9287..10330
                     /locus_tag="IW746_000777"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1077697.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="IW746_000778"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="IW746_000778"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1077698.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="IW746_000779"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="IW746_000779"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1077699.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="IW746_000780"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="IW746_000780"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1077700.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="IW746_000781"
     CDS             13849..14763
                     /locus_tag="IW746_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1077701.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14896..16461)
                     /locus_tag="IW746_000782"
     CDS             complement(14896..16461)
                     /locus_tag="IW746_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1077702.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16454..17905)
                     /locus_tag="IW746_000783"
     CDS             complement(16454..17905)
                     /locus_tag="IW746_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1077703.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="IW746_000784"
     CDS             complement(17919..18935)
                     /locus_tag="IW746_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077704.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /locus_tag="IW746_000785"
     CDS             19155..20243
                     /locus_tag="IW746_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077705.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /locus_tag="IW746_000786"
     CDS             20297..21823
                     /locus_tag="IW746_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077706.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /locus_tag="IW746_000787"
     CDS             21825..23030
                     /locus_tag="IW746_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077707.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23287..24264
                     /gene="guaC"
                     /locus_tag="IW746_000788"
     CDS             23287..24264
                     /gene="guaC"
                     /locus_tag="IW746_000788"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1077708.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24528..25952
                     /locus_tag="IW746_000789"
     CDS             24528..25952
                     /locus_tag="IW746_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1077709.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26413..27522
                     /gene="pdhA"
                     /locus_tag="IW746_000790"
     CDS             26413..27522
                     /gene="pdhA"
                     /locus_tag="IW746_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1077710.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27525..28502
                     /locus_tag="IW746_000791"
     CDS             27525..28502
                     /locus_tag="IW746_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1077711.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9221 a         4877 c         6447 g         8001 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ttctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
     6541 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
     6781 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
     7981 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
     8161 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
     8461 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
     9121 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
     9181 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    10621 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    10681 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagtg tttgcttgga
    12121 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    12901 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    15421 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    16681 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    18061 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    20101 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    21121 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    23101 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    23161 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    23221 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    23281 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    23341 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    23401 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    23461 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    23521 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    23581 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    23641 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    23701 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    23761 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    23821 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    23881 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    23941 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    24001 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    24061 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    24121 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    24181 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    24241 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    24301 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    24361 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    24421 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    24481 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    24541 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    24601 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    24661 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    24721 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    24781 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    24841 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    24901 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    24961 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    25021 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    25081 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    25141 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    25201 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    25261 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    25321 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    25381 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    25441 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    25501 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    25561 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    25621 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    25681 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    25741 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    25801 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    25861 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    25921 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    25981 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    26041 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    26101 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    26161 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    26221 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    26281 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    26341 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    26401 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    26461 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    26521 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    26581 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    26641 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    26701 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    26761 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    26821 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    26881 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    26941 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    27001 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    27061 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    27121 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    27181 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    27241 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    27301 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    27361 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    27421 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    27481 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    27541 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    27601 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    27661 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    27721 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    27781 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    27841 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    27901 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    27961 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    28021 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    28081 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    28141 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    28201 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    28261 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    28321 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    28381 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    28441 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    28501 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACEND010000021        28388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEND010000021 DACEND010000000
VERSION     DACEND010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.022"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(194..991)
                     /locus_tag="IW746_000792"
     CDS             complement(194..991)
                     /locus_tag="IW746_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1077712.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="IW746_000793"
     CDS             complement(988..2172)
                     /locus_tag="IW746_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1077713.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="IW746_000794"
     CDS             complement(2197..3444)
                     /locus_tag="IW746_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1077714.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="IW746_000795"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="IW746_000795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1077715.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="IW746_000796"
     CDS             5424..5738
                     /locus_tag="IW746_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1077716.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="IW746_000797"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="IW746_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1077717.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /locus_tag="IW746_000798"
     CDS             6423..7067
                     /locus_tag="IW746_000798"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1077718.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="IW746_000799"
     CDS             7082..7411
                     /locus_tag="IW746_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077719.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="IW746_000800"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="IW746_000800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1077720.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="IW746_000801"
     CDS             8399..9223
                     /locus_tag="IW746_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1077721.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="IW746_000802"
     CDS             9216..9563
                     /locus_tag="IW746_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1077722.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="IW746_000803"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="IW746_000803"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1077723.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="IW746_000804"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="IW746_000804"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1077724.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW746_000805"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW746_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1077725.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW746_000806"
     CDS             complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW746_000806"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1077726.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15220..15987
                     /locus_tag="IW746_000807"
     CDS             15220..15987
                     /locus_tag="IW746_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077727.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15977..18019
                     /locus_tag="IW746_000808"
     CDS             15977..18019
                     /locus_tag="IW746_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077728.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGITQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18300..18449
                     /locus_tag="IW746_000809"
     CDS             18300..18449
                     /locus_tag="IW746_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1077729.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18460..19974
                     /gene="dltA"
                     /locus_tag="IW746_000810"
     CDS             18460..19974
                     /gene="dltA"
                     /locus_tag="IW746_000810"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1077730.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19971..21176
                     /gene="dltB"
                     /locus_tag="IW746_000811"
     CDS             19971..21176
                     /gene="dltB"
                     /locus_tag="IW746_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1077731.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21221..21454
                     /gene="dltC"
                     /locus_tag="IW746_000812"
     CDS             21221..21454
                     /gene="dltC"
                     /locus_tag="IW746_000812"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1077732.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21457..22725
                     /gene="dltD"
                     /locus_tag="IW746_000813"
     CDS             21457..22725
                     /gene="dltD"
                     /locus_tag="IW746_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1077733.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22866..23948
                     /locus_tag="IW746_000814"
     CDS             22866..23948
                     /locus_tag="IW746_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1077734.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23948..24751
                     /locus_tag="IW746_000815"
     CDS             23948..24751
                     /locus_tag="IW746_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1077735.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24821..25561
                     /locus_tag="IW746_000816"
     CDS             24821..25561
                     /locus_tag="IW746_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1077736.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25572..26576
                     /locus_tag="IW746_000817"
     CDS             25572..26576
                     /locus_tag="IW746_000817"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1077737.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26621..28384)
                     /locus_tag="IW746_000818"
     CDS             complement(26621..28384)
                     /locus_tag="IW746_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077738.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8945 a         5024 c         5848 g         8571 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctatatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt
    12661 ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc
    12721 acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct
    12781 aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct
    12841 ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa
    12901 tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca
    12961 gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca
    13021 cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt
    13081 ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg
    13141 atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt
    13201 gaatagacac tgaaatgata accatattca tccccccatg catctgcatt agcttttaat
    13261 tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg
    13321 aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg
    13381 tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac
    13441 atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga
    13501 agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct
    13561 ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt
    13621 ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc
    13681 ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga
    13741 tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca
    13801 gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc
    13861 ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca
    13921 aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta
    13981 aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt
    14041 tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt
    14101 agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca
    14161 gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg
    14221 gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc
    14281 agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc
    14341 aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt
    14401 ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca
    14461 ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca
    14521 atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg
    14581 atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt
    14641 tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag
    14701 tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt
    14761 tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg
    14821 cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca
    14881 taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa
    14941 tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga
    15001 tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt
    15061 ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa attttttatt
    15121 cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc
    15181 aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg
    15241 aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc
    15301 tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag
    15361 acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc
    15421 caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa
    15481 ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc
    15541 ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat
    15601 gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc
    15661 ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt
    15721 gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg
    15781 aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca
    15841 gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc
    15901 agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc
    15961 ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg
    16021 aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag
    16081 cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg
    16141 tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc
    16201 caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat
    16261 gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc
    16321 ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt
    16381 tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga
    16441 tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat
    16501 atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat
    16561 gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg
    16621 atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct
    16681 tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta
    16741 tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta
    16801 ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg
    16861 gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac
    16921 ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca
    16981 gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat
    17041 aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga
    17101 agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa
    17161 agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga
    17221 tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg gactgccaga
    17281 taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt
    17341 atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga
    17401 ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact
    17461 gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa
    17521 tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc
    17581 acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc
    17641 agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat
    17701 tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga
    17761 aaattatcac ctattgacta agctggggat tacacaaaga aggatcagac ggatgatctt
    17821 cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt
    17881 tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt
    17941 gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg
    18001 tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg
    18061 aataccattt atttggaata agaggttgtg acacgatttt tgtcacaacc tctttatttt
    18121 ttatattttt taaaagtacg agaaattata aagaaaaaag cagtgatttg atgtgctatt
    18181 tttataaaag ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca
    18241 tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa
    18301 tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa
    18361 ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag
    18421 gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt
    18481 gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag
    18541 acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa
    18601 aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc
    18661 tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc
    18721 aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg
    18781 gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc
    18841 aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat
    18901 attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat
    18961 ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc
    19021 ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt
    19081 tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc
    19141 gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt
    19201 tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt
    19261 tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca
    19321 cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt
    19381 gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg
    19441 aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt
    19501 attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg
    19561 ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa
    19621 tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg
    19681 attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc
    19741 gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa
    19801 aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa
    19861 gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca
    19921 gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac
    19981 ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac
    20041 cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga
    20101 cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga
    20161 ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga
    20221 aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa
    20281 cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct
    20341 cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa
    20401 catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac
    20461 caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat
    20521 atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca
    20581 ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc
    20641 atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt
    20701 ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga
    20761 caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt
    20821 ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca
    20881 tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt
    20941 tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt
    21001 atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag
    21061 agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag
    21121 tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa
    21181 tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc
    21241 aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt
    21301 tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga
    21361 gctggatatc caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa
    21421 aatcattcaa caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt
    21481 ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa
    21541 gtagatatct ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg
    21601 ttgaaaggag atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt
    21661 ggctcaagtg aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac
    21721 cagcgaaact atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat
    21781 atgatgatgc gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct
    21841 cctcagtggt tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa
    21901 cttcaaacat acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta
    21961 gctagaagat tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg
    22021 caaacgatca aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa
    22081 tggaacatgc tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag
    22141 caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc
    22201 attttagcaa atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac
    22261 gatttttata cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat
    22321 tgggattatc gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca
    22381 aaaaaccata acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat
    22441 accggactat ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat
    22501 agccaaggat tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg
    22561 gaagacacga tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg
    22621 tttttagaag aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc
    22681 aaatcttggc aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt
    22741 gttgctcgct tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt
    22801 tgcctgaaca aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa
    22861 aacatttgga tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac
    22921 ctggaaatga agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg
    22981 atatttttta tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca
    23041 aggtctttat ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa
    23101 aaggattctt ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc
    23161 aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac
    23221 cacatgtttt aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg
    23281 atattggcgc atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg
    23341 tcactccata tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat
    23401 gggataatcg tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag
    23461 cacacccaaa tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg
    23521 ctcgaacaag tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag
    23581 caggagacac tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac
    23641 agattttgat ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg
    23701 atgtagcaga ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg
    23761 atgcaggaca aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg
    23821 ttcgttatct gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga
    23881 aactagtcac agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt
    23941 atgaataatg aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt
    24001 ggaaaatcaa tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt
    24061 catccagaac ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt
    24121 attgagtttt ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat
    24181 tgcgctagta gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca
    24241 aagaaagtat tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct
    24301 actcaacagt ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca
    24361 gctgcttttc agttcaaatg cggtacctac gggtgtcatg ccagaagatt ttcttgatat
    24421 cagctttgtt ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt
    24481 tggcggttta ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat
    24541 tcaatgtttg aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt
    24601 gcagctgtct tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt
    24661 gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga
    24721 aaaaggaggt ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat
    24781 aacttttgtg gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg
    24841 atgcttagga gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga
    24901 cgggttcaat ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct
    24961 tgatccactt gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga
    25021 ccttggtgta ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct
    25081 ttcaatttat ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt
    25141 ctcaaaagga catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac
    25201 ttttatggaa acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac
    25261 ggggaaagta ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc
    25321 gaaagatgct gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca
    25381 tgcccatttc acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact
    25441 tgtacttaca cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa
    25501 ggcagcaggc aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta
    25561 ggaggaagaa catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa
    25621 ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca
    25681 atgaaccttc aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt
    25741 atgtggaaga aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc
    25801 atgattttgg aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg
    25861 actttgcgaa agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg
    25921 cggaattgaa aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga
    25981 tgtatgctac caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca
    26041 atgaagtagt caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat
    26101 cgatccgttc tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga
    26161 caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag
    26221 aagtaaaaac gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg
    26281 aatattatcg aaattgggat tggaattacg ggaagtctcc agatttcaat ttctctcgcc
    26341 aaaaacgttt ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca
    26401 aagaagcaaa aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa
    26461 aattggttgg acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg
    26521 tgaagaaata ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat
    26581 aaaaaatagc ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat
    26641 ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc
    26701 ctgtgtaacg atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta
    26761 attcttctgt gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac
    26821 aaatgacgaa atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat
    26881 tttcgatggc atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca
    26941 gatcgtcatc gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat
    27001 tccgtttggc agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc
    27061 aaaaagaaca cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt
    27121 tagcttttgt ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt
    27181 ccaggcgaag tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca
    27241 acaaatcatt tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga
    27301 tgtgatcgat tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag
    27361 accaattgat cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga
    27421 taatggctac caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg
    27481 cttcgccaat gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa
    27541 tcggcattaa ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa
    27601 aaatcaaaag ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc
    27661 tccaatagta gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag
    27721 aatgaacgaa tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc
    27781 ctaatgccaa agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat
    27841 acatcaagta gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg
    27901 tcaatacttg cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta
    27961 gataaacttt cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca
    28021 atttttttcc gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc
    28081 tttcttcctg ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc
    28141 attgatggtt tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt
    28201 ccataatgaa tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa
    28261 acttaaaatc aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt
    28321 gattgctgtc cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata
    28381 acaatgat
//
LOCUS       DACEND010000022        28153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEND010000022 DACEND010000000
VERSION     DACEND010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.023"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW746_000819"
     CDS             79..1125
                     /locus_tag="IW746_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1077739.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW746_000820"
     CDS             1474..2106
                     /locus_tag="IW746_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1077740.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW746_000821"
     CDS             2694..4133
                     /locus_tag="IW746_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1077741.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW746_000822"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW746_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IW746_000823"
     CDS             4895..5110
                     /locus_tag="IW746_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077742.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IW746_000824"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IW746_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IW746_000825"
     CDS             complement(5770..6681)
                     /locus_tag="IW746_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077743.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IW746_000826"
     CDS             complement(6700..7704)
                     /locus_tag="IW746_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1077744.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IW746_000827"
     CDS             complement(7701..9824)
                     /locus_tag="IW746_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1077745.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IW746_000828"
     CDS             complement(9829..12276)
                     /locus_tag="IW746_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1077746.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IW746_000829"
     CDS             complement(12263..12652)
                     /locus_tag="IW746_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1077747.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IW746_000830"
     CDS             complement(12712..13215)
                     /locus_tag="IW746_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1077748.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="IW746_000831"
     CDS             complement(13228..13452)
                     /locus_tag="IW746_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077749.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW746_000832"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="IW746_000832"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1077750.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16331)
                     /locus_tag="IW746_000833"
     CDS             complement(16212..16331)
                     /locus_tag="IW746_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1077751.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16346..17530)
                     /locus_tag="IW746_000834"
     CDS             complement(16346..17530)
                     /locus_tag="IW746_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1077752.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="IW746_000835"
     CDS             complement(17789..18079)
                     /locus_tag="IW746_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077753.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW746_000836"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="IW746_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19444..20118)
                     /locus_tag="IW746_000837"
     CDS             complement(19444..20118)
                     /locus_tag="IW746_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077754.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="IW746_000838"
     CDS             complement(20196..21536)
                     /locus_tag="IW746_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1077755.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="IW746_000839"
     CDS             complement(21619..21954)
                     /locus_tag="IW746_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1077756.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="IW746_000840"
     CDS             complement(21964..22338)
                     /locus_tag="IW746_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1077757.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="IW746_000841"
     CDS             complement(22351..22665)
                     /locus_tag="IW746_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1077758.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW746_000842"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="IW746_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1077759.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(26419..27585)
                     /locus_tag="IW746_000843"
     CDS             complement(26419..27585)
                     /locus_tag="IW746_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1077760.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            27610..27936
                     /locus_tag="IW746_000844"
     CDS             27610..27936
                     /locus_tag="IW746_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077761.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
BASE COUNT         8196 a         5759 c         4907 g         9291 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacaag ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat
    26101 agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc
    26161 tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat
    26221 atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga
    26281 aaccaggtct taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat
    26341 accaacgatt tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg
    26401 agaaaggtaa tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg
    26461 ttgagctttt tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc
    26521 caatcgatct tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt
    26581 gtgccggaat ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa
    26641 tatattattt gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata
    26701 ggtaaagata agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc
    26761 ttgttctttt ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg
    26821 ttcggaagat atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt
    26881 ctcttcattt cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata
    26941 aatgtcagac caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag
    27001 ctggaagatt aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt
    27061 tagttcttct aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt
    27121 atttgccata ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga
    27181 gctgtaagac ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt
    27241 ctgacagtga acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg
    27301 caagcggaaa aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga
    27361 aggcaaccat tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt
    27421 atcttcaatc tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa
    27481 cccttttttt acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac
    27541 actatatcgt gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa
    27601 tctgataact tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg
    27661 gatgcaggaa ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt
    27721 tttgaaatga caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca
    27781 tttctaaaag gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa
    27841 tttaaaagaa tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa
    27901 gaatgcaaaa aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg
    27961 atgaagtaga aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca
    28021 ataataaagt tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag
    28081 gtgtgacaat gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt
    28141 ctgatatata tgc
//
LOCUS       DACEND010000023        28035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEND010000023 DACEND010000000
VERSION     DACEND010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.024"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            514..1221
                     /locus_tag="IW746_000845"
     CDS             514..1221
                     /locus_tag="IW746_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1077762.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1280..1924)
                     /locus_tag="IW746_000846"
     CDS             complement(1280..1924)
                     /locus_tag="IW746_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1077763.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2050..2337
                     /locus_tag="IW746_000847"
     CDS             2050..2337
                     /locus_tag="IW746_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077764.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2445..2765
                     /locus_tag="IW746_000848"
     CDS             2445..2765
                     /locus_tag="IW746_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1077765.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2846..4159
                     /locus_tag="IW746_000849"
     CDS             2846..4159
                     /locus_tag="IW746_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1077766.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4156..4875
                     /locus_tag="IW746_000850"
     CDS             4156..4875
                     /locus_tag="IW746_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1077767.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            5003..5554
                     /gene="raiA"
                     /locus_tag="IW746_000851"
     CDS             5003..5554
                     /gene="raiA"
                     /locus_tag="IW746_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1077768.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5776..8310
                     /gene="secA"
                     /locus_tag="IW746_000852"
     CDS             5776..8310
                     /gene="secA"
                     /locus_tag="IW746_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1077769.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8436..9537
                     /gene="prfB"
                     /locus_tag="IW746_000853"
     CDS             join(8436..8498,8500..9537)
                     /gene="prfB"
                     /locus_tag="IW746_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1077770.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9642..10328
                     /gene="ftsE"
                     /locus_tag="IW746_000854"
     CDS             9642..10328
                     /gene="ftsE"
                     /locus_tag="IW746_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1077771.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10312..11205
                     /locus_tag="IW746_000855"
     CDS             10312..11205
                     /locus_tag="IW746_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077772.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11357..12805
                     /gene="cls"
                     /locus_tag="IW746_000856"
     CDS             11357..12805
                     /gene="cls"
                     /locus_tag="IW746_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1077773.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNIGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13142..13999
                     /locus_tag="IW746_000857"
     CDS             13142..13999
                     /locus_tag="IW746_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1077774.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14114..15034
                     /gene="pstC"
                     /locus_tag="IW746_000858"
     CDS             14114..15034
                     /gene="pstC"
                     /locus_tag="IW746_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1077775.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15034..15918
                     /gene="pstA"
                     /locus_tag="IW746_000859"
     CDS             15034..15918
                     /gene="pstA"
                     /locus_tag="IW746_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1077776.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15933..16739
                     /gene="pstB"
                     /locus_tag="IW746_000860"
     CDS             15933..16739
                     /gene="pstB"
                     /locus_tag="IW746_000860"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077777.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16755..17513
                     /locus_tag="IW746_000861"
     CDS             16755..17513
                     /locus_tag="IW746_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077778.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17526..18203
                     /gene="phoU"
                     /locus_tag="IW746_000862"
     CDS             17526..18203
                     /gene="phoU"
                     /locus_tag="IW746_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1077779.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18440..19471
                     /locus_tag="IW746_000863"
     CDS             18440..19471
                     /locus_tag="IW746_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1077780.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19468..20235
                     /locus_tag="IW746_000864"
     CDS             19468..20235
                     /locus_tag="IW746_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1077781.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20335..21903
                     /gene="liaX"
                     /locus_tag="IW746_000865"
     CDS             20335..21903
                     /gene="liaX"
                     /locus_tag="IW746_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1077782.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSVIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22203..22520
                     /locus_tag="IW746_000866"
     CDS             22203..22520
                     /locus_tag="IW746_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1077783.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22520..22870
                     /locus_tag="IW746_000867"
     CDS             22520..22870
                     /locus_tag="IW746_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1077784.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23037..23975
                     /locus_tag="IW746_000868"
     CDS             23037..23975
                     /locus_tag="IW746_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1077785.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23990..24820
                     /locus_tag="IW746_000869"
     CDS             23990..24820
                     /locus_tag="IW746_000869"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1077786.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24864..25889
                     /locus_tag="IW746_000870"
     CDS             24864..25889
                     /locus_tag="IW746_000870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1077787.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25904..26842
                     /gene="galU"
                     /locus_tag="IW746_000871"
     CDS             25904..26842
                     /gene="galU"
                     /locus_tag="IW746_000871"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1077788.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26967..27407
                     /locus_tag="IW746_000872"
     CDS             26967..27407
                     /locus_tag="IW746_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1077789.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27410..28012
                     /locus_tag="IW746_000873"
     CDS             27410..28012
                     /locus_tag="IW746_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1077790.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9281 a         4821 c         6065 g         7868 t
ORIGIN      
        1 ataccacaat cgtaagctct cgttagttcg agagctcttt tttttgcttt gctaccttgt
       61 tccttgaaga ttctgctgat ttaagataga gttaagttaa ctacaagaat tggagggatc
      121 cacatgaaat ggaaagaaaa agtagacgaa gcagctgaaa agttatatga tttggtcaaa
      181 tcagaaaaat acaatattga agtgaatatt ccgaaaaaag gcggaaaagc ggtccgggta
      241 aaatcaaaac gcccaacgaa tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa
      301 caaaagatcc ataagtcggc attttcatcg attttagggt cttttgttat tttctaggtt
      361 ggacatatct aaaaaattgt ttctgacaat aggagtagga agaacgatgt gtgtctaaat
      421 cctctgttga tggatagaaa cctttatctt tttcagttgt gaatcaggta tataccagtt
      481 ataatagaag aaacgaaagg acgggtgtac gttatgtcga atcaattacc tgtatatata
      541 cagattcatg accaattaaa aaatgaaatc gaacaaggta tatggaaagt cggagaccgt
      601 cttccttcag aaagagaatt agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa
      661 gcaattcaaa acttggcaga tgaaggaatc ttagaaagaa agatcggttc gggtacatat
      721 gttgcacgag aaaaagtaca agaaaaaatg tctggtgcaa caagttttac agaaattatg
      781 gaatcacaaa atcgagtacc gtctagtcgg acaatctctt atttcttgac ttcgccaagc
      841 tcaagtgaaa tggaaaaact aaacttgagt aaagatgaca cgatcttgcg aatggaacgt
      901 attcgtttcg cagatgatgt ccctatttgt tttgaagttg ccagtgtgcc acaaaagctg
      961 atcgatggct acagcaaagc agagattact gaatcgttgt atcgcacgct agaagaaaaa
     1021 ggcgggaaaa agatcggtcc agcgaatcaa acgattactg ctgtgttggc ttctgagaaa
     1081 attgctgact atttagaggt aaaacgagga gatgcagttc ttcgtttgcg ccagatttct
     1141 tattttgaag acggtacgcc gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt
     1201 gagttttatt tggaaaaata aaaaacaggt gatccctgat tccgtattag aaataaaaga
     1261 gaatcagaat cacctgtttt tatatatgat cagttaccag ttgctcgtga taagaaacag
     1321 gaccctcttc aaattggact tgtccgttca gcagctcaat gatggattct ttaaagtggt
     1381 cagcactttt ttcatctaca gcacagatga cgctaacttg atcagtatat tggatttctt
     1441 ttataaaaaa atgttggagt tcaagataat tctgtaattt tcctagttgc ggatacgtga
     1501 tcatcacaga gatctcctgt tgcaaggtac cttctacgat tccaatgtca gacaatgctt
     1561 gagagaccgc atgagtgtat gctcgtatta atccgcctgt gccgagtttc gttcctccaa
     1621 aatatcgagt gactactgca gctacattga tcagttgatt ttttttgagg acttccaaca
     1681 tcggtatgcc cgccgttcca cttggttctc catcatcgct gcttcgctgg atttcatttt
     1741 tttctccaat gatgaaggcg ctgcagttat ggttcgcttt ccaatgttct ttttttattt
     1801 cttgaatgaa actttttgct tcgtcctctg attctattcg tctaagagaa cagataaagc
     1861 gtgatttttt gatctcgatc tcactttgtc cattttcttt tattgtacga tagcgctcga
     1921 tcatcttgtc acctcttttt tcattttctt tattcagtat aaataaagtt ctaggttgtt
     1981 tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca aaggcaggtg
     2041 gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac
     2101 aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct gtttcccagt
     2161 aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga ttataaacac
     2221 cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt catcaatttg
     2281 gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt tgattaaata
     2341 aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag taacagacgc
     2401 gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa
     2461 tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa atgcaaaaag
     2521 ctgctgaagc aaaaggaatc gactctgata ttttcgctgt gtccgcttct gacgcagata
     2581 acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt cgctttatga
     2641 aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc atcaatatgg
     2701 ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg ctcataggtg
     2761 aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt
     2821 cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc ttctgacgag
     2881 agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga agatcgcagc
     2941 agtggaacgt ttgagcaact ggattctttg tcatcgctgt ggaatgagaa cgtccgtcat
     3001 tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt tagggcgatg
     3061 cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt
     3121 ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga gaatactttg
     3181 ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga aaacagagat
     3241 gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga tttgtacacc
     3301 gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc cagaagaaaa
     3361 aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt ttgttatctg
     3421 tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga tcgatgagat
     3481 cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa cagcgattaa
     3541 accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac taaaggagat
     3601 caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt tgcttcctga
     3661 acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga agaagataaa
     3721 atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt tattttgtcc
     3781 aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc cgagatatga
     3841 gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga aaatgcgaga
     3901 agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca catttgaaag
     3961 aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt ctttggtaca
     4021 aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat attttttcta
     4081 tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga
     4141 agggaagaag atactgtgag gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca
     4201 ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc agtttgtcgc
     4261 gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc aatccatttc
     4321 aatctagaga gtacgaaaaa gaaagtaaat tctatccccg tttactgcag tgactgtcaa
     4381 gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt tgtgtacgat
     4441 gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg actagcaggc
     4501 acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat ctattgtcca
     4561 atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag cgagatgttg
     4621 aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct gctggctcaa
     4681 gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact tgcagtcacc
     4741 ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac aacaggacag
     4801 actcttttcc acgcagcaga ttgtttgcag atcgcacagc cgaaaatcat ccgaactatt
     4861 tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc aaataaccta
     4921 gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt cttgattgtg
     4981 gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa atatcgaagt
     5041 aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac gctatttcag
     5101 tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa
     5161 agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa cttctccaga
     5221 tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc gcaaattcaa
     5281 aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcaaatg ctgctgcttt
     5341 attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac gtacgaaacg
     5401 cctttcattg aaaccaatgg atagtgaaga agcagtcttg caaatgaaca tgctgggaca
     5461 taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt accgccgtaa
     5521 agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa
     5581 acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct cttttagatg
     5641 aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc aattatctat
     5701 aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca gaaggaaaag
     5761 gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga taagaaagaa
     5821 ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca aatggcagca
     5881 ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata ccaaaaagga
     5941 gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc agcaaaacgt
     6001 gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga
     6061 aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat gccagtatat
     6121 ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata tttagcaacc
     6181 cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt ggggttgaat
     6241 atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat cacttatagt
     6301 acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta ccgccatcaa
     6361 atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat cttgattgat
     6421 gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc tttatataca
     6481 agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat cgatgtacaa
     6541 tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac attcggtttg
     6601 gataacttat acgacattga aaatactgca ttgacccatc acttggatca agcattgaga
     6661 gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa agtactgatt
     6721 gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg acttcaccaa
     6781 gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc
     6841 actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg tacagctaaa
     6901 acagaagaag aagaattcag agaaatttat aacatccaag tataccagat tccgacaaat
     6961 cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca aagcaagttc
     7021 cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt gttagtcgga
     7081 actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga acgtattcct
     7141 catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat gaatgctggt
     7201 caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga tatcaagtta
     7261 ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg acatgaatct
     7321 cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca
     7381 cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga acgaatcaaa
     7441 gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag caagatgctg
     7501 acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga cacacgtaaa
     7561 aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga
     7621 caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat ggtcaaacgg
     7681 acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaactg gaatttggaa
     7741 agtatcatcg attttgccgg cagtactctt gtccatgaag atagtattac tcttgcagat
     7801 atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc acaagaagta
     7861 tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt tgaaaaagtc
     7921 gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgacgcaat ggatcaatta
     7981 cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga atatcaaaca
     8041 gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt cactcgaatc
     8101 tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca aggaacagct
     8161 tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca
     8221 atccatgtga acaaggtcgg aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat
     8281 aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt gagacaaatt
     8341 ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt tagactagat
     8401 ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa attcgaaatc
     8461 tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga cttagatcag
     8521 ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt ttgggatgac
     8581 agcgaagccg cacaaaaagt gatcagtgaa aataacgcta ataaagaaac ctatgaccaa
     8641 ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat gctgcaagaa
     8701 gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt agatgaaaaa
     8761 atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa caatgcgatc
     8821 gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag catgctttta
     8881 cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact agattatcaa
     8941 gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca taacgctttt
     9001 ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc ttttgactca
     9061 gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct tgatgaaacg
     9121 atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc aagtggtgca
     9181 ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt gcctacagga
     9241 acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca agcgatgggg
     9301 atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga agcagctgca
     9361 cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta tgttttccat
     9421 ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt ccaagcagtt
     9481 atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct acaataagaa
     9541 cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa cattgccatg
     9601 ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa atgaaggatg
     9661 taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac
     9721 aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca ttcatcaaat
     9781 tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa
     9841 tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg gtgttccaag
     9901 actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta
     9961 tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac cttgttggtt
    10021 tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag caacgtgtct
    10081 ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa ccgacaggaa
    10141 atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc aatgcccaag
    10201 gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt cggcaccgag
    10261 taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa tatggatacg
    10321 atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa acgtaacggc
    10381 tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt cggtgtattc
    10441 atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa cgttgatgta
    10501 tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga aaaagaattg
    10561 aatgacttag atcatgttaa aaaagttgaa ttttcaagta aagaacagga attgaaaaaa
    10621 atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa cccgttatat
    10681 gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc agaagaagca
    10741 gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga tcggattttc
    10801 caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct gctgttcgta
    10861 gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca gcgtgaaatt
    10921 caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt tttccttgaa
    10981 ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac atttggttat
    11041 gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc attgattcat
    11101 ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt gatcattggt
    11161 tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca caatataaat
    11221 aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct aggagaatag
    11281 aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt aagttggtag
    11341 aggaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc ttttgattat
    11401 gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg
    11461 ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat atattttttt
    11521 aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga aaatcggtaa
    11581 gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc ctcatccgcc
    11641 tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg aaagtacgct
    11701 ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat tcgatgcgct
    11761 gattgaagat atcaagcaag cagaagacca tatccacttc cagtattata tttatcgcag
    11821 tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac gaggggtaaa
    11881 agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct tttttcagaa
    11941 tttgaaaaaa gcaggcggag aaatcgcatt tttctttcct ctatttgttc cgtatattaa
    12001 tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga aaatcggata
    12061 tacaggtggg ttcaatattg gaaatgaata tctaggattg gtaaagaaat ttggttattg
    12121 gcgagacaat catttgcgta tttacggaga agcggtgtat atcttgcaaa accgtttttt
    12181 gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat tttttccgtc
    12241 cattcactct actggtgata tagctgttca aatcgtgaca agcggaccag ataccgaaca
    12301 tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg agatattgat
    12361 ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt tggctcttct
    12421 ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc tcgtttactg
    12481 ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg aaacatatga
    12541 gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt cagttggctc
    12601 agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac tgatttatga
    12661 tgaacgaatg gcttcacgtg taagaaaagc attttttgaa gactcgaaaa tttctacgca
    12721 tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag aaggattagc
    12781 aagattgatt tcgccgctcc tttaaaacac aaaaaggaca tgagaaccat caacagatgg
    12841 agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa tggagaaagc
    12901 gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa agcaagaaat
    12961 aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat agaaactttt
    13021 aatgagatag ttttttttac aattatgagg atttattttg ttatttaatg attttgattc
    13081 gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa ggagatttct
    13141 aatgaaaaaa cttaaattat tcttaccaat aataggatta gctattcttt tatcaggctg
    13201 ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg ctttacagcc
    13261 tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat tcatcaatgt
    13321 acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg
    13381 aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat tagtcgatca
    13441 tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg taaaagacat
    13501 cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga gtgaagtcgg
    13561 tggaaaagat cagccagtag ttatattgaa ccgtgcttca ggaagcggaa cccgtagcac
    13621 gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac aagattcaag
    13681 cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg ttgctttttc
    13741 ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga cagatgaaaa
    13801 tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa gagatcatcc
    13861 aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac aagataacat
    13921 cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg
    13981 aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa cttttacatg
    14041 ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa acctgattaa
    14101 gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa
    14161 aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg tgttggttgt
    14221 attatctatt ttttattttg tagctagtaa aggattggcc acctttttta ctgataagat
    14281 caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg gagcaaatgg
    14341 gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga cctttttatc
    14401 tgctattgtt gccacaccat ttgccattgg tgcagcagta ttcatgacgg agatctctcc
    14461 taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta ttccttctgt
    14521 cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta tttttggcgg
    14581 gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct tgccgactgt
    14641 cacaacgatg actgttgatg cattaaaggc agtaccacgg cactatcggg aagcttcatt
    14701 agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc
    14761 cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag ccttagctat
    14821 ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc ctgcttctac
    14881 attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag ttgaaaataa
    14941 tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta atatcgtgat
    15001 ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc agataaaatt
    15061 gctacaggtg ttttatatgt agtttcgggg atcatcgtat tgattttagc agctttacta
    15121 ctttatatct tagttcgggg aataccgcac atttcatggt ccttcttgac cgaaccttcc
    15181 aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat ttatttgctt
    15241 ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta tctttcagaa
    15301 tatgctggaa aaaactggct gacagatgtg attcgcacgt cgattgaaat cttgagctca
    15361 ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca attccagtat
    15421 ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc tcttttgact
    15481 agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc aggattggct
    15541 ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc gacaccaggg
    15601 attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc tgcattgatc
    15661 tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc attaagtgtt
    15721 tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat ttggaagatc
    15781 aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg
    15841 atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg attataccga
    15901 aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg aatgaaacaa
    15961 atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat ctacatgtct
    16021 ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa aataagatca
    16081 ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa
    16141 tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa ggcgtagata
    16201 ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg gtttttcaac
    16261 gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg aaacagcacg
    16321 gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct
    16381 tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct ggcgggcaac
    16441 agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg
    16501 agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca cttgtaaact
    16561 tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagcg gctcgtatca
    16621 gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa actaacaaga
    16681 tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat tttggttaag
    16741 acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat ctgtattatg
    16801 gaaagaaaga agcactaaaa gggatcgatc taagtttcaa tcaaggggaa atcactgcga
    16861 tgatcggtcc ttctggttgc gggaaatcca cttatctgcg ttctttgaac cggatgaatg
    16921 acctgattcc gaatgtaacg atcaccggga gtgttttata taaagacaaa gacatctatg
    16981 gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc cagcagccaa
    17041 acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta aaaggggaaa
    17101 aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg tctgtttggg
    17161 atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg cagcagcagc
    17221 gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg gatgaaccaa
    17281 caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta gaattaagag
    17341 agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt atttcagata
    17401 agacggcatt ctttttagat ggtcacttga tcgaatacaa tgatacgaag aagatattca
    17461 tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga taaggaggaa
    17521 acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat cagttctacg
    17581 aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg
    17641 ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac atggaaacac
    17701 gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca actgatttgc
    17761 gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc gatcatgcgg
    17821 tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg gaaatcgaaa
    17881 aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc ctagttgctt
    17941 atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa agagtcaatg
    18001 actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag
    18061 tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc ggagactatg
    18121 tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact gagttaaata
    18181 cgaaccacaa tgagattttc taaactaaag cggtgattct gccgtttgtt taggaaaaga
    18241 ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag aacagtcaaa
    18301 caaaaatagc tcctcatatt tttcatttgg ctggacttat taccgcagat gcaagctttt
    18361 gaacactgtt tattcgggaa agggtttgtg acaaaatatg tcacaaaccc ttttcatcat
    18421 ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat tttttatttg
    18481 tgtgcttact tcatgggtta ttcagcatca agctgcttta tatgaagtac ctgtcatcga
    18541 gatcgaaaaa gtacgaataa aagaggatca tcaattgatt acaggaagac tgatgaacac
    18601 gaatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc aaatagaaac
    18661 agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg aatggcagct
    18721 agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca ttggcgccat
    18781 gttgcttatt ggcggaaaac atggtctatt tgctttagga agtctcatca tcaatatcgt
    18841 tttactttta cttttgttgc tcatctttac taaaatagaa gggatttctc tattaaatat
    18901 atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa
    18961 tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat
    19021 ttgtcttttg acaatgaatc tttggaaaga ccaaggactg cgctatgagg aactgaatta
    19081 tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg
    19141 aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc
    19201 agaaataaca gcgaaacagt tatggctttc gggaaggcaa gtgggtcgag atgtgattgg
    19261 ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt
    19321 atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt
    19381 tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt
    19441 ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta
    19501 gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc
    19561 atcaatttag ggatcttttt cttgttgtta ttcggttatc atcagaagtg gccgatactg
    19621 gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt
    19681 tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta
    19741 ctattgcttc ctatcactga ttttttagca atccaaggtt ttacaacgat agaactagaa
    19801 gaattatctg gcttagataa gactttagcg attgatttta gattacttac gagatcgttg
    19861 cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc tagtgggaca
    19921 ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc
    19981 gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga
    20041 agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc
    20101 aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt gtccgcacta
    20161 cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa
    20221 ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat
    20281 aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa
    20341 gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta
    20401 gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa agaagcagac
    20461 aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa
    20521 aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag acaagatcaa
    20581 gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt
    20641 ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa
    20701 gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca
    20761 cagcgtcaga cgttagaagc agaaatcaaa gagttagaag ctagtggcga tacactgtta
    20821 gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag
    20881 aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca
    20941 gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggaaaattc
    21001 ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat
    21061 gtcagcttgc gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa
    21121 gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg
    21181 tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct
    21241 gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc
    21301 ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta
    21361 tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct
    21421 aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg
    21481 atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat
    21541 tctgttatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct
    21601 gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta
    21661 gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa
    21721 ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt
    21781 cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa
    21841 atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa
    21901 taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag
    21961 cttcccctat tttacaaaat tgtgccttat tgccgatagg ctatctcttg aacaccgttt
    22021 accaagagat tatgaacttg ccaattatcg ccgagtcaca aggaataatt tcaaaaaaag
    22081 ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcaaaa agacaccgat
    22141 taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca
    22201 tgatgaaaaa aaagttgaca aaatccaata aaaatgttgt tttgacaggg actttagcag
    22261 gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga
    22321 gcttagttgc ttttggaagt ccaatcattt tatacatttt attagctttg attatcccat
    22381 ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt
    22441 atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg
    22501 acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt
    22561 catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt
    22621 tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact
    22681 gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat
    22741 tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc
    22801 agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg aacataacag
    22861 tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga
    22921 atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt
    22981 tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt
    23041 cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg
    23101 acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt
    23161 tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa
    23221 tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta
    23281 gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa gaaatgatcc
    23341 aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact tctcgcttat
    23401 taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg
    23461 ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa
    23521 gtgaaacagc gttggagttg atcaaacgcg gtcatcgatt gatcgcagac gatcgagtag
    23581 atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc
    23641 tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg
    23701 tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga
    23761 aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagta ggcattccac
    23821 aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga
    23881 tgaatttccg tgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa
    23941 gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca
    24001 aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat
    24061 cgtatcaggt atcattatcg ccatgtggct tagcagcaga gaggctgttc gggtaggatt
    24121 aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca ttattggcgc
    24181 tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt
    24241 caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct
    24301 gtattttttt actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc
    24361 aagtgtatta ttagcacaag caatcggtcg ttggggaaat ttcatgaacc acgaagcata
    24421 tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga
    24481 taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag
    24541 tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg
    24601 tgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt
    24661 acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt
    24721 gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga
    24781 ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg
    24841 aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt
    24901 ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac
    24961 atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag
    25021 atcattgtct acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg
    25081 cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag
    25141 tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggga
    25201 cacataaaag gatctctgac atcttgttag aggaaatccc agtagaaaaa agaaaagctg
    25261 ttgttgttct ttctggacca agccatgcag aagaagtagc gaaacatgac atcactacga
    25321 tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata
    25381 attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga
    25441 aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa
    25501 aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg
    25561 ccaacccgct gacctttatc ggattaagcg gagtaggcga tctagtcgtt acttgcacaa
    25621 gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag
    25681 aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg
    25741 aactagccca aataatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt
    25801 atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat gggaaaaaag
    25861 aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag tcaaaaaagc
    25921 agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa
    25981 ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa
    26041 atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca
    26101 ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt
    26161 gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa
    26221 gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac catttgtcgt
    26281 tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat taatcgatga
    26341 ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc
    26401 aaaatacgga attatcaatc caggagaagt attggaagat gggttataca atgtcaaaaa
    26461 ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta ttggacgtta
    26521 cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag ccggaaatga
    26581 aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa
    26641 attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa caagtattga
    26701 atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc tgtctttagg
    26761 taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa taacagaaat
    26821 caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct aattgaataa
    26881 aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa
    26941 aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag
    27001 caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa
    27061 ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa atcgtcacaa
    27121 aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt aatgaactgc
    27181 tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt
    27241 taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg
    27301 gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg acagcgatga
    27361 aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa tggctaaaaa
    27421 aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg ccgcgttatt
    27481 gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag cggatgattt
    27541 gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg agttaacagg
    27601 tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag caggtagtgt
    27661 caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg atatcttgtt
    27721 aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg cagaagatat
    27781 gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag atatcataat
    27841 tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc
    27901 aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga
    27961 agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact aaaatcacaa
    28021 atttatagaa taaaa
//
LOCUS       DACEND010000024        27904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEND010000024 DACEND010000000
VERSION     DACEND010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.025"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..634)
                     /gene="plsY"
                     /locus_tag="IW746_000874"
     CDS             complement(<1..634)
                     /gene="plsY"
                     /locus_tag="IW746_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1077791.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKK"
     gene            761..1195
                     /locus_tag="IW746_000875"
     CDS             761..1195
                     /locus_tag="IW746_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1077792.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1361..3406
                     /gene="parE"
                     /locus_tag="IW746_000876"
     CDS             1361..3406
                     /gene="parE"
                     /locus_tag="IW746_000876"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1077793.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3418..5868
                     /gene="parC"
                     /locus_tag="IW746_000877"
     CDS             3418..5868
                     /gene="parC"
                     /locus_tag="IW746_000877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1077794.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6145..8376
                     /gene="pflB"
                     /locus_tag="IW746_000878"
     CDS             6145..8376
                     /gene="pflB"
                     /locus_tag="IW746_000878"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1077795.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8620..9381
                     /gene="pflA"
                     /locus_tag="IW746_000879"
     CDS             8620..9381
                     /gene="pflA"
                     /locus_tag="IW746_000879"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1077796.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9466..10392
                     /locus_tag="IW746_000880"
     CDS             9466..10392
                     /locus_tag="IW746_000880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1077797.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10574..11467
                     /locus_tag="IW746_000881"
     CDS             10574..11467
                     /locus_tag="IW746_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1077798.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11601..11726
                     /locus_tag="IW746_000882"
     CDS             11601..11726
                     /locus_tag="IW746_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077799.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11908..13455)
                     /locus_tag="IW746_000883"
     CDS             complement(11908..13455)
                     /locus_tag="IW746_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1077800.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(13557..13910)
                     /gene="tnpB"
                     /locus_tag="IW746_000884"
     CDS             complement(13557..13910)
                     /gene="tnpB"
                     /locus_tag="IW746_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1077801.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(13900..14094)
                     /locus_tag="IW746_000885"
     CDS             complement(13900..14094)
                     /locus_tag="IW746_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077802.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(14213..14845)
                     /locus_tag="IW746_000886"
     CDS             complement(14213..14845)
                     /locus_tag="IW746_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077803.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14993..15535)
                     /locus_tag="IW746_000887"
     CDS             complement(14993..15535)
                     /locus_tag="IW746_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1077804.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            15670..16059
                     /locus_tag="IW746_000888"
     CDS             15670..16059
                     /locus_tag="IW746_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077805.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            16063..16827
                     /locus_tag="IW746_000889"
     CDS             16063..16827
                     /locus_tag="IW746_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1077806.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            16956..17903
                     /locus_tag="IW746_000890"
     CDS             16956..17903
                     /locus_tag="IW746_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1077807.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            18033..18773
                     /locus_tag="IW746_000891"
     CDS             18033..18773
                     /locus_tag="IW746_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077808.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(19064..19732)
                     /locus_tag="IW746_000892"
     CDS             complement(19064..19732)
                     /locus_tag="IW746_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1077809.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            19866..20153
                     /locus_tag="IW746_000893"
     CDS             19866..20153
                     /locus_tag="IW746_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077810.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            20569..22107
                     /locus_tag="IW746_000894"
     CDS             20569..22107
                     /locus_tag="IW746_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1077811.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            22104..23069
                     /locus_tag="IW746_000895"
     CDS             22104..23069
                     /locus_tag="IW746_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077812.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            23056..24378
                     /locus_tag="IW746_000896"
     CDS             23056..24378
                     /locus_tag="IW746_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1077813.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      24441..24545
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24619..25362
                     /locus_tag="IW746_000897"
     CDS             24619..25362
                     /locus_tag="IW746_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077814.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            25377..26369
                     /locus_tag="IW746_000898"
     CDS             25377..26369
                     /locus_tag="IW746_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077815.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            26381..27118
                     /locus_tag="IW746_000899"
     CDS             26381..27118
                     /locus_tag="IW746_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077816.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            27108..27830
                     /locus_tag="IW746_000900"
     CDS             27108..27830
                     /locus_tag="IW746_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1077817.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         9513 a         4819 c         5738 g         7834 t
ORIGIN      
        1 attttttctt gctgctattc aaaccaaacg ggattcgact ttcagtatgg tttctgatac
       61 gcttgatatt gtctttatgc cgataaaaga taaaggaaga aactaaaaag gcaatgatcg
      121 tcagtaacca attgggctta gccaaaatca caggagctac ataaggaagg acgatcgtag
      181 ataaagtgat caatactgca ctgatcatac tgctcaaact caccatactt gtacaaaata
      241 aactaacgat aaagatgata caagaataaa caaaaaatac aggattaaat cctaaaagca
      301 ttcctgcact tgtcgcaaca gcttttcctc ctttgaaccc agcaaaaatc ggaaatgtat
      361 ggccaagaat ggcgcatacc ccaaaccaaa gagggttaat cccagaaagg tggaagatca
      421 tcggcaaaca agtagccaat gtacctttta aaatatccat aagcaggaca acaatcccgg
      481 ctgttttgcc taatacacga aatgtattgg tcgttccagt attaccgctc ccgtgttgtc
      541 ggatatcttt tttgaaaaac aatttgccaa tccagacacc ggaaggaata gaacctaaaa
      601 gatatgctaa aataagtaat aagatcaatt tcatttttta ccctctttca agttgaaaac
      661 tatcctttat tttagcatga ataggtaaca ggaacaattg aaaaaatgaa agatacaagt
      721 ataatagatg gaataaaaag aaaagcaagg ggtggcagaa atgtctgtaa aaaatccaac
      781 acaagaaacg attttcaact atttggaaga agcaaaaaat attgccgtcg taggcttgag
      841 caataaacca gaacgaacaa gttacatggt tgcacaggta ttgcaagaca atggatataa
      901 aatcatacca gtaaatccat tattggctgg tgaagaaatt ctaggcgaga aagtctatgc
      961 gaatttgaca gaaatcgatg atccaatcga tatcgtagat gtatttagac ggagtgaatt
     1021 tttacctgaa atagcaggtg attttttgaa aactgatgct aaagtgttct gggcacaatt
     1081 agggattgaa aacaaagaag cagctgagat gctaagaaaa gcaggacgta cagatatcgt
     1141 aatggatcgt tgtattaaga ttgaacttgg taagttgaaa gaaaaccaaa aataaaaatg
     1201 accacaaaga agagatgatc gatagagggg aatgtcgaaa aagcggttag gagataatta
     1261 aaattgattt ctcgtctacc ctcttttttt tcacgacctt ttctagtatg ataatcgtgg
     1321 tgcggttttg ccgcaaagta ttattgaagg agcgtttgcc ttggcaaaaa aagtaaacaa
     1381 cgaatacaat gactcgtcca tccaagtatt ggaaggatta gaggcagtta gaaaaagacc
     1441 agggatgtac attggatcca ctgacagccg aggcttgcat catttagtct atgaaatcgt
     1501 cgataatgca gttgacgaag cattatcagg ttatggcagt gagattgatg tgaccatcca
     1561 tgaagacaat agtataactg ttgcagatag cggacgtggg atgcctgtag gaatgcatgc
     1621 ttccggaata ccaacagttg aagtgatttt tactgtgctg catgctggcg gaaagtttgg
     1681 tcaaggaggg tacaaaactt ctggtggact gcacggtgta ggagccagtg tggtaaatgc
     1741 cttatcgaaa tggctaattg taacgatcgt tagagatggc gtagagtatc agcagaaatt
     1801 taaaaacggc ggtaaacctg acggtacttt gaaaaagata ggaaaaacaa aaaaagcaaa
     1861 cgggacgact gtccattttt taccagatga tacaatattc agtacaacga aattttcata
     1921 tgaaatttta gccgaacggt taagagaatc tgcctttctt ctaaaaggcg ttaaaatctc
     1981 tctttctgat cttcgtggag aagaaccagt caaggaaatt tttcattacg aagaaggaat
     2041 caaagaattt gttgattatc tgaatgaaga aaaagataca ttgacaccag ttgtttattt
     2101 ttcaggagaa aaagaaggta tcgaagtaga ggtcgcttat caatacaatg acggttattc
     2161 agaaaatgtt ttatcttttg tcaataacgt tcggacaaaa gatgggggaa cccatgaagc
     2221 tggtatgaaa gcggcgatga cgaaatctta taatgaatat gcgagaaaag ttggtttgct
     2281 gaaagaacgg gacaagaatc tggaaggtag tgatttcaga gaagggttag ctgctgtact
     2341 ttctatccgt gtacctgaaa atcttctgca atttgaaggg caaacaaaag aaaaacttgg
     2401 tacgccagtt gctcgtaccg tagtagataa cgtcatcagt gaacaaatgg gcttttattt
     2461 gcaggaaaat agcgaaatga gccagatgtt ggtccgtaaa gcaatcaaag ccagagaagc
     2521 gagagaagca gcacgtaaag ccagagaaga aagccgaaat gggaagaagc gtaaaaaagg
     2581 cgaatctctt ctttcaggaa aattgacacc cgcacaatcc cgaaacccta agaaaaatga
     2641 attgtattta gtcgaagggg attctgctgg cggatctgct aaacaaggaa gagacagaaa
     2701 gttccaagca attctacctt tacgaggaaa agtcatcaat accgaaaaag ctaaaatgca
     2761 agatatcttg aaaaatgaag aaatcaatac gatgatctat acgattggtg caggagtcgg
     2821 tcctgagttt tctatcgaag actgtaatta cgacaaagta atcatcatga ccgatgcgga
     2881 tacagacggc gcccatattc aagtattact gcttactttc ttttatcgtt atatgaaacc
     2941 gctgattgaa gctgggaaag tatatatcgc gttaccgcct ttatataaag tatccaaagg
     3001 acaaggaaag aagcaagtca ttgaatatgc gtggacagat gacgaattgg cagcaatgat
     3061 taaaaaagtc ggcaaaggat atatgctgca gcgatataaa ggacttggag agatgaatgc
     3121 ggaacaattg tgggaaacaa cgatggatcc gacttcccgt acgttgatcc gcgtccgtat
     3181 cgatgatgct gcgcaagctg aacgtcgagt aaccacactg atgggtgaca aagtagaacc
     3241 tcgaagaaaa tggatcgaaa atcacgtgca atttacgctc gaagaagatg gcagtatctt
     3301 agataaaaaa gaggatactg aaatctctcc ttcagtttct aatgatttat tagatgaaga
     3361 acgagcagat aaaaatgaaa acaatcagtt atttgaagta gaataggagg tcacctgatg
     3421 gaaaatcgac aagaagtaca agaattaact ttagaagaag tgatgggtga ccgcttcgga
     3481 cgttattcca aatatattat ccaagaacgt gcactgcctg atatccgtga tggattgaag
     3541 ccagtgcaac gccggatatt atttgccatg aacaaagatg gcaatacgta tgacaaagga
     3601 ttccgtaaat cagcaaaatc tgtcggaaat atcatgggga attatcatcc tcatggtgac
     3661 cgcagtattt atgaagcgat ggtccgcatg agccaagact ggaagttgcg ggaagtactg
     3721 atcgaaatgc acgggaataa cgggagtatg gacggtgatc cacctgctgc gatgcgttat
     3781 actgaagcca gattatccaa gttaagtgga gagatgctag cagatatcga gaaagaaaca
     3841 gtcgatctcg tgtggaattt tgatgataca gaaaaagaac caacagtcct tccagctcga
     3901 tacccaaacc ttctcgtcaa tggatcgaca ggtatatctg ctggatatgc aacagaaatc
     3961 ccgactcata acttagctga agtgatcgat ggcacaatct atatgatcga tcatccacaa
     4021 gcgagccttg aaaaattgat ggaatatatt cctggacctg actttccaac aggagggatc
     4081 ttgcaaggaa aagatgaaat caaaaaagct tatgaaacag gacgaggaaa agttattctt
     4141 cgttcgaaaa caaagataga ggcaatgaag ggtaataaac agcaaatcgt gatcacagaa
     4201 atcccatatg aagtaaataa agcgaccttg gtgaaaaaaa tggatgagat ccgtttgaat
     4261 aagaaaatcg atggaatagc tgaagtacga gatgaaagtg atagaacagg tctgcaaatt
     4321 gtcgtcgaat tgaaaaaaga cgccaatgct caaggaattt tgaattattt gttcaaaaac
     4381 actgagctac agatcaatta caatttcaat atggtcgcta tcgaccatat gactccgcat
     4441 caagttggac tgaaagacat tttaagaagt tatatcgagc atcgaaagca agtcataaca
     4501 aaacgtagtc aattcgattt agccaaagct caaaaaagac agcatatcgt agaaggattg
     4561 atgaaagctt tatcgattct ggatgaagta atcgcaacga ttcgtgaaag taaagacaaa
     4621 aaagacgcca aaaagaattt agtcgacgtc tttcaattta cagaagaaca agcagaagca
     4681 attgtcacat tgcaattata ccgtttgacg aacacggata tcacagagct tcaaaaggaa
     4741 tccgaaagct tgattgcgca aatcacagag ttgaataaaa tcctttcaaa tgacaaagag
     4801 cttttctctg ttatgaaaaa agaattgcga gaagtgaaaa aaaattattc ttccgctcgt
     4861 ctaacgacaa tcgaagacga aatcgaagaa atcaaaattg atacgcaagt cttagtggca
     4921 caagaagacg taattgtcag tgtgacgcga gaaggctatg tgaagcgaac aagccttcgt
     4981 tcttatagtg cttccaaacc agaagaaatc gggatgcgag aaggcgacta cttgctatac
     5041 tcaggtgaat tgagtacatt ggaccatgtt cttctgatca caaataaagc aaatgtgatc
     5101 taccgccctg tccatgaact acccgatttg aaatggaaag atgctggtga gcacatttct
     5161 caaacaatct ccaatctgtc tgtagacgaa tcgattttag ctgttttccc atatcaaaaa
     5221 attcaagcag agaaaacatt tgtattcatc agcaagaatg gattgatcaa gcaaacacga
     5281 atgacagagt tcgaaccatg gcgcacctat aagagccgtc cgctcagctg tatgaagcta
     5341 aaggcggaag aagatgaact gattgctgtg tatctgaaag aagatcagcc aggcttagat
     5401 gttttcttag taacgcatca aggaatgggt ttgcgctatc ctttaacaga tgttcccgtt
     5461 gttggagcaa aggcagctgg agtgaagtct atgaatttga aagatggaga taggattatc
     5521 aacgggctgc ttgtgttagc agaaggtgat acgccgatcg taatcgttac tcagcgagga
     5581 gctgttaaac gcatgcttgc tcaagaaatc agccaaactt cacgtgcgaa gcggggagtg
     5641 acggtcatgc gtgagctgaa aaaaaatccg caccgtatta tctatatgtc ggaaggaaga
     5701 tcaaaagaaa taaccttgat gaatcaaaaa gggcagcagt taacgattga cccaactgat
     5761 ttccctatag gtgatcgaac atcgaatgga tcatttgcga tggatgagaa aaaaggtgga
     5821 gaagtcatcg aagttatcga tgctcctgag attttcatcg agtcgtaact gttgtagtat
     5881 tttaatgaag agacgccatc tgtattaata caggtggcgt ctctttttca ttcattttta
     5941 ataaatcctt atacgtttgc aaaaaaagat gaaagtgttc acaatgctta aatgttggta
     6001 tgtcaacgtt gatttaaata tatgtaaata aaacataaaa attgttaaat aaaaaacaag
     6061 aaaacccttg caaagatagg gtgctgtgtt atactaatca cgtaaaaact gttacataaa
     6121 acagaaattt aggaggtcca tacaatggag caatggaatg gatttaaagg taccaaatgg
     6181 aaagaaggcg tagacactag agactttatt caaaataact acactgaata tcgcggtgat
     6241 gacagctttt tggaaggccc aacagaagca acagaaaaat tatgggatga attgcaaaag
     6301 ttaaatgata tccaattcga gcgtaacggt gtctatgata tggacacaga aatcgtatca
     6361 actattactt cacacgatgc aggctatttg gataaagatt tggaaaaaat cgtcggtcta
     6421 cagacagaca aacctttgaa acaagcattc atgccgttcg gcggaatcaa tatggctaac
     6481 aatgcgctaa aaagcaatgg ctatacacca aacgatgaat tgacacatat tttcactgac
     6541 tggcgcaaaa cacataatca aggtgtattt gacgcttata ctgctgaaat gcgtgctgct
     6601 cgtaaaaaca aaatcattac agggcttcca gacgcttacg gtcgcggacg tatcatcggt
     6661 gactatcgtc gtgtggcatt gtatggaatc gactatttga tggaacaaaa gaaaaaagat
     6721 catgatatga ctggttatca tacaatgagt gaagaagtga ttcgtttacg tgaagaaatc
     6781 actgaacaat atcgtgcatt agcgcaaatg aaagaaatgg ctgcgaaata tggctatgat
     6841 atttctaaac cagctgcaaa tgcaaaagaa gctgtccaat ggctatactt cggatattta
     6901 gcagcaatca aatcacaaaa tggtgctgcg atgtcaatcg gacgtatctc agcattcctt
     6961 gatatctata tccaacgtga tttggataat ggagtaatca cagaaaaaga agcacaagaa
     7021 ttgatcgacc acttagtaat gaaattacgt atggtcaaat tcgcgcgtac acctgaatat
     7081 aaccaattat tctcaggatt ccctatctgg gctactttat ccattgcagg tatgggatta
     7141 gatggtcgtt cattagttac aaagaatgat ttccgtctgc ttcatacatt aacaaatatg
     7201 ggaccttcac cagaaccaaa cttgactgtt ttgtattctg aacaccttcc agaaggattc
     7261 agaacatatg ctagcagaat cgctatcgaa agttcttcga ttcaattcga aaatgataat
     7321 cagcttcgtg aacaatgggg ttctgatgat tgtgcgattg cttgctgtgt atcaggaaca
     7381 gttgtcggaa aagacatgca attctttggc gctcgtgcta acttggctaa aacactatta
     7441 tatgccatca atggcggagt cgatgaagtg actggcgccc aagttggacc agaatatcgt
     7501 ccaatcacat cagaaatact agactttgaa gaagtgaaac ataacttcat gaatatgatg
     7561 gattggttag cagaactata tgtcaatacg ttgaacatca ttcattatat gcatgataaa
     7621 tattcttatg aagcagctca gctggctttg atggattctg acttgcaacg cacatttgct
     7681 acaggtattg ctgggatctc tcatgcggca gacagcttgt cagcgatcaa atatgcaaaa
     7741 gtaaaagcaa tccgtaatga aaaaggaata gcagttgatt ttgaagtgga tggcgacttc
     7801 cctaaatacg gaaatgatga tgaccgtgca gatgaattag tttcatggat cttggaatac
     7861 ttcatgggac aaatcaaacg tcataaaact taccgcaact caaaaccaac aacttcatta
     7921 ttgacgatta cttcaaatgt tgtttacggt aaaaatacag gaaatactcc tgatggccgt
     7981 gaagctggaa aaccacttgc gccaggagca aatccaagtt acaatgcgga acaaagtggg
     8041 ttattagcta gcctgaactc aacagctaaa atgaattacg gctgtgcatt ggatggaatt
     8101 tcaaataccc aaacaatcaa tccttcaggt cttggaaaag aacaagatac aagaatcgaa
     8161 aacttgcgta atgtcttaga tggatacttc tcaaaaggag cttatcattt gaatgtcaat
     8221 gtattcagta atgatttatt gcgtgatgca atggaaaatc caatgaaata tccgaacttg
     8281 acgatccggg tatctggtta tgcagttaaa ttccgtgact tgacaagaga acaacaatta
     8341 gatgtcttga tgagaacttc acacgacaga atgtaaaaag ttcgtgaaaa aagtttttcg
     8401 acttagcgtc gaaaggacgt tgaatagcca tcgcatcaaa atcgaataat cagtatgctt
     8461 atttgaaaga aacgatttac aggggcagga cttgtatgtt ccgtccctgt aaattttaca
     8521 cataacgaag cggcaattgc gaaaaatttt gttcagaaag cttcgcttcg acagctaact
     8581 tgtacttaaa atgaaatcaa agaaaagatg tgaacttaaa tggaagaaaa aacaatcggc
     8641 tatgttcatt ctatcgaaac tttcggttca gtagacggac ctggactgcg ctttgtcgtg
     8701 tttatgcaag gatgccgcat gcgttgccaa ttttgtcaca atccagatac ttggaatatt
     8761 ggcggaggga aagaatacac agcagatgaa cttttagata aagcagaacg cttcagacct
     8821 tactggggag ataaaggtgg tattactgtt agtggtggag aacctctatt gcagatcgat
     8881 tttttgatcg aactatttaa aaaagcaaaa gagcggaaga tgcatactac attggataca
     8941 tgcggaaaac cttttacgta tgaagaacct ttcttttcac gttttcaaga attgatgaag
     9001 tatacagatt tactgttatt tgacatcaaa cacatcgata atgaagagca taaaaaacta
     9061 acgcattggg acaatgaaaa tattttagaa atggctcaat atttgtcaaa aatcaacaaa
     9121 ccggtttgga ttcgccatgt actagttcct gagcgaagtg attatgatga atatttgatc
     9181 cgactagaca actttattca aaccttgtca aatgtagaca gagtagagat tcttccttat
     9241 catacgatgg gaaaatacaa gtgggaaaca cttggtttga aatatccatt agaagggatc
     9301 gaaccaccta caaaagaacg tgtcgaaaat gctaaacgat tgcttcatac agaagagtat
     9361 acaaaatatt taacgcgata ggtcgcatcg gaaagaagat aagtattgct tctttccgtt
     9421 tttttgctat tattaaatag gagaaatcac aaaggagcga atgacatgtc aaaaatttta
     9481 gtttttggtc accaaaatcc agatacagat gcaatcgggg cagccattgc gttttcacac
     9541 ttgcaaaatg aattaggtaa agaaacagaa gcagttgctc taggtacacc taatgaagaa
     9601 acacaatacg cattagacca ctttcagcta tctgcaccac gagtagtaga gtcagctaaa
     9661 gcagaaaatg cagatgtcat gcttgtggat cataatgaat tccaacaaag tatttcagat
     9721 atcagcgaag taaatatttt agctgtagta gaccaccatc gtattgcaaa ctttgaaaca
     9781 gctaatccat tgtactatcg tgcagaacca gttggatgta caagtacgat tattttaaaa
     9841 ctatataaag aaaaccaagt aacaattcct aaagaaatcg caggaatcat gctctcagct
     9901 atcatttcag atacattgtt gttcaaatca ccaacatgta cgcctgaaga tgaagcagct
     9961 gcaaaagaac ttgcagcaat cgctgaagtc gatctaaaca cttatggact tgaaatgttg
    10021 aaagcaggaa caaatctaag tgataaatca gctgcggttc ttcttgattt ggatgcaaaa
    10081 agtttcccaa tgggcagttc caacgttcgt atcgctcaag tcaatacagt ggaccttaat
    10141 gaagtgatgg atcgacaagc agagctagaa gcagcaatgg aagatgaaat gaaagcaaat
    10201 ggctacgatc tgttcgtatt agttgtcaca aacatcttag acagtgattc agaattactt
    10261 gttgctggta aaggaaaaga aaaagtagaa gaagctttcc aaacaacttt gaccaacaac
    10321 cgtgctttgc ttaaaggtgt cgtttcacgt aaaaaacaag tcgtacctca attgacggct
    10381 gtatttagct aatcaaatgg tagtatgaag cattcaaaat gaatggataa gcgattgata
    10441 taaaaagaaa catagaacat actaaaggcc gaagaaatgg aggagaataa cagtcaatta
    10501 tgactcttgt tcttcctcct ttttacgtaa aagcctttta agtaaaataa aacggataga
    10561 aaagagaaca tatatgaact attattcaaa aaataaggaa aacttttatc aaaaactgac
    10621 cgatgatccg cttttttctt cattgaccga ttatctatat gaacatcgag aacaagaaac
    10681 gattctaaga gaattgaaaa aagaattttc gcaaaataaa ttttcccatt ttcttgattt
    10741 attgatcgat gcaggattaa tcaagaggga agaacgaaga tatcatttga actttccaat
    10801 atttgatcct aaagattact tgcaacaagc cacttcggct gctgaaacca tagctgaaca
    10861 attgaagaga ctctctgttg atgaacaaaa attagcgatg ggagaagtta tttgggcgta
    10921 ttgtttcgaa gatgaacgga aagaagcgta tttttatgga gtaagaaaca gccgagagac
    10981 agaattgctg aggacaactg caggaaatga gaagtaccgt tttatcacac tttcctctat
    11041 agaacacttc ccgcttactt tggctaatta cttttttgta cagaagaatc agcttcctgt
    11101 aacgaaggca ttcaaagaat tagccgaact tattggagac gtaaatgaat cgtacttttt
    11161 tgatcaaata gaagttatcg ttgacaggat acgtaagaat aaatataaaa atcgccggcc
    11221 tagtattttc catcagtctc tacttgtgac gaatactata aaggaagaag aatcattcac
    11281 attagaatta cctattgtcg aaaaaaacaa ttttgagatt gaattaccaa cattggatcc
    11341 ttcattgaca atggaggaaa tggcctttct taaacgacaa atcttttctg agctgagcaa
    11401 gaaatttata ccgcatgcat tttcttatat caaagaatat ggaacagttt tagtgagtaa
    11461 aacctaagag agcagtaagc aaaaattata ctgactcttg caggaggagc ctttctcttc
    11521 ctttatggtt aaaagaatta tttgagtatt ttgcctatct accacctatt agattcacta
    11581 catggtctgg aaacaaagga atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag
    11641 tagtgataag aaaagttaga tttttaaata atcaatatta tgattcagtc aagtatggca
    11701 ttttatggga agaactagct gattagatta ggaagtccat ttgataatcc agaatcacta
    11761 agaaacgaat ggcgagtcat taataattta tccaatcttg ggttattctt ttagattgaa
    11821 gtgtttaaaa cgtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata
    11881 gaatagaatc gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt
    11941 gttccaaggc aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt
    12001 atcaaataga aactctagat actttacagg attcaatccg ttaagttctg ctgttttata
    12061 tacactcaga atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt
    12121 tttccgtcct atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa
    12181 acgaccatct tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc
    12241 tgcatccaac ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat
    12301 aaataatgga cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt
    12361 ccatttcttt tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat
    12421 atccgtgttt ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa
    12481 acaatagaca agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa
    12541 ataccctgta aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg
    12601 gtgataataa acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt
    12661 atctgttttg gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc
    12721 ttgattaaga tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt
    12781 aatatgccaa ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata
    12841 gttctcttgc cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac
    12901 taaactattg gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa
    12961 agaagaaagt ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag
    13021 acaatccaac atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc
    13081 tttcatctgt gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt
    13141 ctctgttata ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc
    13201 ttttactgga ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact
    13261 aaaaagattc aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa
    13321 tttgttggtt aaataattga ccttttcatt caatagttgg atttcagttg taagattctg
    13381 aatggtttgt tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt
    13441 atctgtagtt tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc
    13501 acaagaaacg cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa
    13561 aaaactcctt tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac
    13621 aaccatcgaa cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt
    13681 aacttgccgt tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga
    13741 agtttgaaac ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg
    13801 agttgatact cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt
    13861 tttccgcaca caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag
    13921 ttctttcaaa atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga
    13981 accattctct cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg
    14041 tgcactcttt tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa
    14101 cctccagact tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt
    14161 tctatggggc gcttacttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt
    14221 caaaatgctt tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga
    14281 tgaagagcat atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca
    14341 ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc
    14401 gaaagaagag cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa
    14461 tggataaaat cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg
    14521 aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt
    14581 cgtttaacgt accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta
    14641 agccgcaaag agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag
    14701 aatgatccaa aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg
    14761 aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat
    14821 tagtggttct acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt
    14881 ataataacaa attgatgagt atatgtcgat gttttttaat caaaataata aataaaaact
    14941 gtctaacatt taaaatttaa tttagatatt agacagtttt taagtttgtg agttattttc
    15001 cttgtcgttt tctcactttt ttagtccaga aaccacctga aatatatttt ggctcataag
    15061 aaatgataaa agcacgctct tcgatttctt tgatcaggcg atacagttca cgttcgcttt
    15121 ttctaggaga aagtatttcg agaactagcc gctcaccttc cagccccatt gcagaagtct
    15181 gtgtcacacc ataaccgtgt tctctcagta tacccggcaa acttttatct tctgcgctat
    15241 ttgctggcaa aatcgttgtg accatgatgt atcctaatgc caatttgtct tcaatcaaaa
    15301 ttccgacact gatacctact gcgtaaccta acgcatagac aaataaattc agtggattat
    15361 ctaaacgatc taaaacaagg gataagccga cgatataaat cgtgatctcg accatactga
    15421 caagtggagc aatcagtcga tagcctttca ttgtcaacat gaaacgtaac gtgttcagag
    15481 tgatataagc aaaattaatg aggaagatca tccccaacat ttttggatca atcatttgta
    15541 aaactccttt catttatata acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga
    15601 aactttatac atacaaaaaa atgataaaat gattataata gaattataaa gataggttag
    15661 gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt
    15721 ctcagtatat acttaggtgt ctattcgatt caacagccaa ttgaggtaga gaacattgtt
    15781 tttcagatgt cgctgacaga tggtttaatt ttaagcgttt ttttcttttt gatactgttt
    15841 attggtaata ttttcggtag cgttgccagt tttttgaatt tttctattta tccactcctt
    15901 tcgctagcat taggagtggt gggactttgt tctctgtgct tatttcccga gccattcagt
    15961 cctgcattta tattatttgg tacactgaac ctgttccaga caactgttgg agcatggttg
    16021 ctatggcgat caggaaattt aatgaaaata ggtgagtaac aattgaatcc aattatattg
    16081 aaacgttctg tccatcatat tccagtgtta gaagttgttc tgaaagaatt aagagaggaa
    16141 cgtattcccg ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt agttttgacg
    16201 caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat
    16261 ggggagcgca aacagccggt ttcttctatt ccatcctata cattttggga tagtatctac
    16321 ggaaatctgt ttgaatttga cacacttatc aagcatttag aaaaaagagg tcttctacat
    16381 gatcagcttg cagttggcgg ggtttcgatg gggggaatga caacatgcgc cttgttggct
    16441 aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat
    16501 ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa agactatttt
    16561 gatttgattt catgggttaa gagctatgat ttgtcattgc agccggaaaa gttagctgga
    16621 cgtcctctgt tcttttggca tggacaagct gacgaaaaaa tcccttatat acaaacggaa
    16681 cgatttgtag aaaaaaatcg gcataagtct tacggaaaag aaattgtttt tcgttcctca
    16741 aaaacacagc gacatatggt acaaatacca ctaatggaag aagcttccac ttttttagca
    16801 aaaaatttga taaaaaagta taaataattg aaacggaaaa tcactcatga cgatagagga
    16861 ttttccgttt tttattttgg gctataataa actctgttag gaaaatttgg tataattgat
    16921 agtaagaatg acaacatgtt agaaaggaag agagcatgcc cactttagca gatgttgcaa
    16981 aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat caatcatccg gaacaggtga
    17041 ctgacgaatt gaaagagtta gttttctcag caatggctga actggattac cgtccaaata
    17101 ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat caaattattt atactggaag
    17161 aaatcgacac aactgagccc tattacatga atttgctgat gggaatcgct aaacggatcg
    17221 gtaaagcgca ttattctctc caattagtta caaatgatgc cttcgacgtc ggttcttgtg
    17281 atggatacat catcacaggt gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa
    17341 aaccagttgt cttgtttggg gaaaattctg aaggatatga ttatgtagac agcgataatg
    17401 aatatggtac agcaaaagca acgcaatatg ccttagaaag agattatcaa acgattattt
    17461 atatcggtat cgaagtggac gagccttttg aaatttcaag ggaaactggt tatttatctg
    17521 tgatgaagga agttgatctt cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag
    17581 tagaaagata tatgacagaa aactgggcag attttacaga taaaacttgt tttgtatgta
    17641 gttcagatcg gctagcacta ggagcagtaa gagggattat aaatgctgga ggacacttgc
    17701 ccgaagaatt tggtgtgatt ggatttgatg gggtattttt agaccaaatc agttcaccta
    17761 aattgacaac gatcaagcaa gatattatac gtatgggtga agtatgtgga gaaatgttgt
    17821 taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt cctgaactag
    17881 ttatcagaga gacaacgaat taagtcttat tactatatat atatttatga aattcatcgc
    17941 tattttctta aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg ctattattga
    18001 gaaaagttcc atttttgaga aggatggttt gaatgaaaga aatcagacga aaacatttcc
    18061 gttttcctgc gtacgatgat gagttaggcg taaaattaac caacgacaag aagcgagtat
    18121 tatttgacgg acaagaagat cttttgactg aacagaatca gtccccgttt gaacagatga
    18181 aagaatttca tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa
    18241 atggattgaa gtcagctccg cgtaaggaaa atttggaaaa gcataaagga aaattaccgg
    18301 attataccgg ccattaccaa tcagaaatca catcgacagg acgaaaaaaa ctttttggtg
    18361 attcctctcg ttctgtcaga caaagtgaaa caaggacaga gtccaagcat actacacgaa
    18421 acgcatctgt tgacacgaag gtgcgggagc gttcttattt tgttcctaaa tatgtacccg
    18481 catcgattat cccagaccct agagaaagca aaatttctga ggatcattta atcgaatcga
    18541 tgaaaaaaac ggatgactct tatctgttgt tcgatacaga accgacgccg tatcaagaaa
    18601 aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa
    18661 agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc tcattacaag
    18721 gattgatcga tgatcaagga aactctttgg gagaaaatag ctattttaaa taagtgatat
    18781 acaaatttat attcggctac agttggaaag gaaacgctgc ggcgtttcct tattttatac
    18841 tctatccgaa agtttatatc gtatcctcaa atttttttga gtttaagcga attttttctc
    18901 ccaataagta agaaaataat ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc
    18961 aaaaatagtt tattttttta taaagaagat tttactgaat aatatcattt tagtttacat
    19021 aatgatattt ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc tctaactgtt
    19081 caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat tggggcaaaa
    19141 cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata gaacagctaa
    19201 gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact aaaataccaa
    19261 aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca atggcttgat
    19321 aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc
    19381 cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca aaatgtatat
    19441 gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga
    19501 gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa ttaaaaagca
    19561 gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca ataccagtaa
    19621 ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat ccgtagattc
    19681 ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc atttctagcc
    19741 tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac tcttacttca
    19801 agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa ataaggagtg
    19861 gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa aaaacagagt
    19921 atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg gtccagataa
    19981 ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag
    20041 acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag
    20101 aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa
    20161 taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag
    20221 tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga
    20281 tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata
    20341 tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga
    20401 gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag
    20461 gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa
    20521 aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt
    20581 atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc
    20641 atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca
    20701 gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt
    20761 ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca
    20821 ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta
    20881 gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga
    20941 cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact
    21001 gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta
    21061 gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg
    21121 atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag
    21181 aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct
    21241 ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg
    21301 gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa
    21361 gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt
    21421 caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct
    21481 gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta
    21541 ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc
    21601 ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca
    21661 gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga
    21721 tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca
    21781 agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca
    21841 gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca
    21901 gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt
    21961 aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga tatgaattac
    22021 caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa
    22081 ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa
    22141 agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct
    22201 ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat
    22261 tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc
    22321 tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca
    22381 tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa
    22441 gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag
    22501 atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta
    22561 ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt
    22621 caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg
    22681 ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga
    22741 aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg
    22801 actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt
    22861 tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct
    22921 ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag
    22981 tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc
    23041 gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc
    23101 ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat
    23161 ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca
    23221 gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact
    23281 gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc
    23341 cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg
    23401 gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca
    23461 accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca
    23521 gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt
    23581 gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat
    23641 aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg
    23701 gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt
    23761 actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg
    23821 actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat
    23881 aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt
    23941 ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat
    24001 tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct
    24061 acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct
    24121 tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat
    24181 gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca
    24241 gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa
    24301 ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc
    24361 tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt
    24421 gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag
    24481 ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag
    24541 acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag
    24601 aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt
    24661 ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga
    24721 cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc
    24781 acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact
    24841 gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt
    24901 tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt
    24961 atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt
    25021 tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca
    25081 gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc
    25141 ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc
    25201 gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa
    25261 gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca
    25321 ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga
    25381 aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag
    25441 aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg
    25501 taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg
    25561 ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag
    25621 gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag
    25681 atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat
    25741 tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg
    25801 caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg
    25861 gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa
    25921 aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg
    25981 gtattgaact aaactatatc ccattgaaag aattagatga acggttagat tactatactc
    26041 cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta
    26101 tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac
    26161 tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct
    26221 tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc
    26281 gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag
    26341 aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca
    26401 cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa
    26461 agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac
    26521 tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac
    26581 tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag
    26641 cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca
    26701 agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca
    26761 attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc
    26821 ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca
    26881 agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga
    26941 tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca
    27001 tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca
    27061 atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg
    27121 ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt
    27181 tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa
    27241 gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg
    27301 aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata
    27361 tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg
    27421 cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa
    27481 attatatcca agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc
    27541 aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc
    27601 cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg
    27661 gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac
    27721 tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta
    27781 aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg
    27841 tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat
    27901 taat
//
LOCUS       DACEND010000025        27637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEND010000025 DACEND010000000
VERSION     DACEND010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.026"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            121..768
                     /locus_tag="IW746_000901"
     CDS             121..768
                     /locus_tag="IW746_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1077818.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            852..1796
                     /locus_tag="IW746_000902"
     CDS             852..1796
                     /locus_tag="IW746_000902"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1077819.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1931..3382
                     /locus_tag="IW746_000903"
     CDS             1931..3382
                     /locus_tag="IW746_000903"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1077820.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3403..4239
                     /locus_tag="IW746_000904"
     CDS             3403..4239
                     /locus_tag="IW746_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1077821.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4239..4898
                     /locus_tag="IW746_000905"
     CDS             4239..4898
                     /locus_tag="IW746_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1077822.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5024..5713
                     /locus_tag="IW746_000906"
     CDS             5024..5713
                     /locus_tag="IW746_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077823.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5719..6675
                     /locus_tag="IW746_000907"
     CDS             5719..6675
                     /locus_tag="IW746_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1077824.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6719..7231
                     /locus_tag="IW746_000908"
     CDS             6719..7231
                     /locus_tag="IW746_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1077825.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7487..8035)
                     /locus_tag="IW746_000909"
     CDS             complement(7487..8035)
                     /locus_tag="IW746_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1077826.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8124..9305)
                     /locus_tag="IW746_000910"
     CDS             complement(8124..9305)
                     /locus_tag="IW746_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1077827.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9541..11049)
                     /locus_tag="IW746_000911"
     CDS             complement(9541..11049)
                     /locus_tag="IW746_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1077828.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11208..11399
                     /locus_tag="IW746_000912"
     CDS             11208..11399
                     /locus_tag="IW746_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077829.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11602..12519
                     /locus_tag="IW746_000913"
     CDS             11602..12519
                     /locus_tag="IW746_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1077830.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12512..13585
                     /locus_tag="IW746_000914"
     CDS             12512..13585
                     /locus_tag="IW746_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077831.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13733..14251
                     /locus_tag="IW746_000915"
     CDS             13733..14251
                     /locus_tag="IW746_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077832.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14232..14624
                     /locus_tag="IW746_000916"
     CDS             14232..14624
                     /locus_tag="IW746_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1077833.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14700..15635
                     /locus_tag="IW746_000917"
     CDS             14700..15635
                     /locus_tag="IW746_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1077834.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15768..16547
                     /locus_tag="IW746_000918"
     CDS             15768..16547
                     /locus_tag="IW746_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1077835.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16540..16857
                     /locus_tag="IW746_000919"
     CDS             16540..16857
                     /locus_tag="IW746_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1077836.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17101..18711
                     /locus_tag="IW746_000920"
     CDS             17101..18711
                     /locus_tag="IW746_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1077837.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18868..19716
                     /locus_tag="IW746_000921"
     CDS             18868..19716
                     /locus_tag="IW746_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1077838.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19947..20426
                     /locus_tag="IW746_000922"
     CDS             19947..20426
                     /locus_tag="IW746_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077839.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20459..22687
                     /locus_tag="IW746_000923"
     CDS             20459..22687
                     /locus_tag="IW746_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1077840.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22816..24459
                     /locus_tag="IW746_000924"
     CDS             22816..24459
                     /locus_tag="IW746_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1077841.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(24532..25077)
                     /locus_tag="IW746_000925"
     CDS             complement(24532..25077)
                     /locus_tag="IW746_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1077842.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25100..25585)
                     /locus_tag="IW746_000926"
     CDS             complement(25100..25585)
                     /locus_tag="IW746_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1077843.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25575..26387)
                     /locus_tag="IW746_000927"
     CDS             complement(25575..26387)
                     /locus_tag="IW746_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1077844.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26526..>27637)
                     /locus_tag="IW746_000928"
     CDS             complement(26526..>27637)
                     /locus_tag="IW746_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1077845.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9050 a         5027 c         5526 g         8034 t
ORIGIN      
        1 tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa
       61 aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca
      121 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
      181 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
      241 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
      301 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
      361 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
      421 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
      481 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
      541 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
      601 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
      661 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
      721 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
      781 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
      841 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
      901 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
      961 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
     1021 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
     1081 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
     1141 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
     1201 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
     1261 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
     1321 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
     1381 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
     1441 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
     1501 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
     1561 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
     1621 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
     1681 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
     1741 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
     1801 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
     1861 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
     1921 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
     1981 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
     2041 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
     2101 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
     2161 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
     2221 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
     2281 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
     2341 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
     2401 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
     2461 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
     2521 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
     2581 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
     2641 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
     2701 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
     2761 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
     2821 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
     2881 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
     2941 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
     3001 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
     3061 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
     3121 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
     3181 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
     3241 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
     3301 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
     3361 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
     3421 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
     3481 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
     3541 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
     3601 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
     3661 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
     3721 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
     3781 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
     3841 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
     3901 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
     3961 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
     4021 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
     4081 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
     4141 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
     4201 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
     4261 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
     4321 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
     4381 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
     4441 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
     4501 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
     4561 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
     4621 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
     4681 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
     4741 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
     4801 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
     4861 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
     4921 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
     4981 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
     5041 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
     5101 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
     5161 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
     5221 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
     5281 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
     5341 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
     5401 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
     5461 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
     5521 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
     5581 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
     5641 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
     5701 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
     5761 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
     5821 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
     5881 cgaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
     5941 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
     6001 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
     6061 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
     6121 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
     6181 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
     6241 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
     6301 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
     6361 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
     6421 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
     6481 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
     6541 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
     6601 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
     6661 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
     6721 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
     6781 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
     6841 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
     6901 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
     6961 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
     7021 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
     7081 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
     7141 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
     7201 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
     7261 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
     7321 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
     7381 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
     7441 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
     7501 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
     7561 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
     7621 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
     7681 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
     7741 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
     7801 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
     7861 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
     7921 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
     7981 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
     8041 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
     8101 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
     8161 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
     8221 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
     8281 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
     8341 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
     8401 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
     8461 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
     8521 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
     8581 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
     8641 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
     8701 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
     8761 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
     8821 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
     8881 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
     8941 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
     9001 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
     9061 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
     9121 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
     9181 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
     9241 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
     9301 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
     9361 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
     9421 aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa
     9481 aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt
     9541 ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag
     9601 ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc
     9661 ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat
     9721 tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt
     9781 tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc
     9841 gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga
     9901 acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg
     9961 atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac
    10021 gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc
    10081 ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat
    10141 ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt
    10201 cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat
    10261 acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta
    10321 ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc
    10381 ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt
    10441 ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc
    10501 gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat
    10561 atactcattt tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc
    10621 ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac
    10681 tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata
    10741 accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc
    10801 acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc
    10861 tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt
    10921 cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt
    10981 cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat
    11041 ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg
    11101 tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct
    11161 agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac
    11221 agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa
    11281 tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc
    11341 taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa
    11401 aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc
    11461 gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt
    11521 tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat
    11581 gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac
    11641 gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat
    11701 atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa
    11761 aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat
    11821 gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag
    11881 atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca
    11941 acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat
    12001 gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc
    12061 caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact
    12121 gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact
    12181 gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa
    12241 ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag
    12301 acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag
    12361 cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa
    12421 gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct
    12481 aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac
    12541 aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac
    12601 aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga
    12661 aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat
    12721 aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt
    12781 gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc
    12841 agattttcca caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc
    12901 cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa
    12961 tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc
    13021 gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat
    13081 cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga
    13141 aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt
    13201 attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc
    13261 aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt
    13321 gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc
    13381 aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt
    13441 agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat
    13501 ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt
    13561 cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata
    13621 attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta
    13681 tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac
    13741 aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt
    13801 tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt
    13861 aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt
    13921 tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt
    13981 ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt
    14041 ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg
    14101 tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat
    14161 aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc
    14221 aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca
    14281 ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa
    14341 aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag
    14401 aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca
    14461 aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac
    14521 tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat
    14581 tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg
    14641 ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca
    14701 tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc
    14761 tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg
    14821 aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac
    14881 aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt
    14941 tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag
    15001 cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa
    15061 tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca
    15121 aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg
    15181 aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag
    15241 caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc
    15301 tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga
    15361 tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc
    15421 ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag
    15481 ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg
    15541 atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa
    15601 cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa
    15661 aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt
    15721 tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga
    15781 aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac
    15841 cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag
    15901 gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta
    15961 cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata
    16021 cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg
    16081 tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg
    16141 acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg
    16201 ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg
    16261 tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac
    16321 tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc
    16381 aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg
    16441 agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg
    16501 agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta
    16561 cttttagttg tttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat
    16621 tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca
    16681 ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct
    16741 tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg
    16801 atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact
    16861 gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac
    16921 tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg
    16981 ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt gtccttctat
    17041 gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa
    17101 atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa
    17161 gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt
    17221 atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt
    17281 ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga
    17341 gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt
    17401 atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt
    17461 gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc
    17521 ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga
    17581 acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac
    17641 ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct
    17701 ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc
    17761 ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc
    17821 tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc
    17881 gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc
    17941 gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca
    18001 ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg
    18061 atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg
    18121 ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc actatacaca
    18181 ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct
    18241 ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt
    18301 tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga
    18361 ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc
    18421 tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc
    18481 agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc
    18541 gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat
    18601 tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat
    18661 gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa
    18721 tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta
    18781 ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa
    18841 attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca
    18901 tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt
    18961 atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt
    19021 ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt
    19081 ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca
    19141 gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg
    19201 gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc
    19261 ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc
    19321 cgagataata cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga
    19381 cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca
    19441 ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct
    19501 cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac
    19561 gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa
    19621 tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt
    19681 gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc
    19741 aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac
    19801 gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc
    19861 ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa
    19921 aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag
    19981 taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa
    20041 aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca
    20101 aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac
    20161 gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc
    20221 gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga
    20281 accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt
    20341 attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac
    20401 gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga agagaagcat
    20461 ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg
    20521 gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa
    20581 agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg
    20641 gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa
    20701 agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt
    20761 agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact
    20821 tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga
    20881 aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag
    20941 ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga
    21001 gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa
    21061 tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat
    21121 cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat
    21181 gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc
    21241 gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt
    21301 ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta
    21361 ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt
    21421 gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag
    21481 tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc
    21541 tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga
    21601 aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa
    21661 tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca
    21721 aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga
    21781 ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact
    21841 gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt
    21901 tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat
    21961 gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga
    22021 gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg
    22081 tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc
    22141 tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa
    22201 agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg
    22261 agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga
    22321 agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg
    22381 tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa
    22441 actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa
    22501 agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt
    22561 ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt
    22621 tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta
    22681 tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct
    22741 agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta
    22801 aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa
    22861 atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc
    22921 cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc
    22981 tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag
    23041 agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc
    23101 aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa
    23161 tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat
    23221 ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat
    23281 gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa
    23341 agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata
    23401 gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc
    23461 acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat
    23521 ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact
    23581 gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt
    23641 gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa
    23701 cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg
    23761 gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg
    23821 gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt
    23881 tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat
    23941 gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa
    24001 gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact
    24061 tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc
    24121 aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag
    24181 aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat
    24241 cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt
    24301 attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag
    24361 tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta
    24421 gcaccttatg aagctcggtt atataaaata aacaagtaaa acttcctcgt taaagttcat
    24481 tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt
    24541 cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc
    24601 aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga
    24661 tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt
    24721 gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga
    24781 gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa
    24841 atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt
    24901 acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac
    24961 ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa
    25021 tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt
    25081 ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag
    25141 ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag
    25201 ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa
    25261 ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac
    25321 cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc
    25381 ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac
    25441 cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag
    25501 ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat
    25561 agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag
    25621 acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt
    25681 ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca
    25741 gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat
    25801 acctcataat gatcaattga atagagaaga tccacagctt catctccagc catccttgtg
    25861 ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc
    25921 atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta
    25981 ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga
    26041 ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt
    26101 tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca
    26161 attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat
    26221 tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc
    26281 aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct
    26341 ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc
    26401 atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat
    26461 ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta
    26521 aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt
    26581 gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt
    26641 tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata
    26701 atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac
    26761 gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact
    26821 tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc
    26881 caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc
    26941 cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact
    27001 gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca
    27061 cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg
    27121 ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt
    27181 gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct
    27241 ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca
    27301 attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc
    27361 atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact
    27421 ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc
    27481 catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt
    27541 gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca
    27601 gacaaagcag gttgttccat taaataaaga ataggaa
//
LOCUS       DACEND010000026        26396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEND010000026 DACEND010000000
VERSION     DACEND010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.027"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(341..3490)
                     /locus_tag="IW746_000929"
     CDS             complement(341..3490)
                     /locus_tag="IW746_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1077846.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3504..4748)
                     /locus_tag="IW746_000930"
     CDS             complement(3504..4748)
                     /locus_tag="IW746_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1077847.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(4741..6333)
                     /locus_tag="IW746_000931"
     CDS             complement(4741..6333)
                     /locus_tag="IW746_000931"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1077848.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(6842..7567)
                     /locus_tag="IW746_000932"
     CDS             complement(6842..7567)
                     /locus_tag="IW746_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077849.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7721..8047)
                     /locus_tag="IW746_000933"
     CDS             complement(7721..8047)
                     /locus_tag="IW746_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077850.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8084..8449)
                     /locus_tag="IW746_000934"
     CDS             complement(8084..8449)
                     /locus_tag="IW746_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1077851.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8486..8701)
                     /locus_tag="IW746_000935"
     CDS             complement(8486..8701)
                     /locus_tag="IW746_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077852.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            8830..9165
                     /locus_tag="IW746_000936"
     CDS             8830..9165
                     /locus_tag="IW746_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077853.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9241..11238)
                     /locus_tag="IW746_000937"
     CDS             complement(9241..11238)
                     /locus_tag="IW746_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077854.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11336..11611)
                     /locus_tag="IW746_000938"
     CDS             complement(11336..11611)
                     /locus_tag="IW746_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077855.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11843..12577
                     /locus_tag="IW746_000939"
     CDS             11843..12577
                     /locus_tag="IW746_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1077856.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12616..13758
                     /locus_tag="IW746_000940"
     CDS             12616..13758
                     /locus_tag="IW746_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1077857.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13853..15244)
                     /gene="sufB"
                     /locus_tag="IW746_000941"
     CDS             complement(13853..15244)
                     /gene="sufB"
                     /locus_tag="IW746_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1077858.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15249..15719)
                     /locus_tag="IW746_000942"
     CDS             complement(15249..15719)
                     /locus_tag="IW746_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1077859.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15706..16941)
                     /locus_tag="IW746_000943"
     CDS             complement(15706..16941)
                     /locus_tag="IW746_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1077860.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16938..18224)
                     /gene="sufD"
                     /locus_tag="IW746_000944"
     CDS             complement(16938..18224)
                     /gene="sufD"
                     /locus_tag="IW746_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1077861.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18237..19010)
                     /gene="sufC"
                     /locus_tag="IW746_000945"
     CDS             complement(18237..19010)
                     /gene="sufC"
                     /locus_tag="IW746_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1077862.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19207..20055)
                     /locus_tag="IW746_000946"
     CDS             complement(19207..20055)
                     /locus_tag="IW746_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1077863.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20079..20765)
                     /locus_tag="IW746_000947"
     CDS             complement(20079..20765)
                     /locus_tag="IW746_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1077864.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20758..21792)
                     /locus_tag="IW746_000948"
     CDS             complement(20758..21792)
                     /locus_tag="IW746_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1077865.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22187..22525)
                     /locus_tag="IW746_000949"
     CDS             complement(22187..22525)
                     /locus_tag="IW746_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1077866.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22527..22883)
                     /locus_tag="IW746_000950"
     CDS             complement(22527..22883)
                     /locus_tag="IW746_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1077867.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22889..24076)
                     /locus_tag="IW746_000951"
     CDS             complement(22889..24076)
                     /locus_tag="IW746_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1077868.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24267..25112)
                     /locus_tag="IW746_000952"
     CDS             complement(24267..25112)
                     /locus_tag="IW746_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1077869.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25346..26311
                     /locus_tag="IW746_000953"
     CDS             25346..26311
                     /locus_tag="IW746_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1077870.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7548 a         5472 c         4450 g         8926 t
ORIGIN      
        1 ggttacatgg tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cactttatct
       61 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      121 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      181 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      241 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccactttac
      301 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattttcaa
      361 gtggaatcag ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt
      421 taaatggctt cgcttctgga tggttggctt tatatccttc gtaatcctta ctgttgataa
      481 ttgctttcat attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta
      541 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
      601 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
      661 caaacgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
      721 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgccgcataa gttcctagta
      781 attgatcaat ataagtagaa tcaatatcat acaacttcgt ggtattttca ctataaaatt
      841 cgatcgtgga aaaatcttct ccctcttttt ctggttcttc ctctattaat gaacccttca
      901 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaggttgaag
      961 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1021 attcttggaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1081 attccatgga tgaaacctct tttaacttac catatgcttg cttgaatctt tttttggatt
     1141 cctgataact tggataaatc aaacccgatt cttcttttgc ttctgaatac agttttgtcg
     1201 catcctcgac attcttctcc attgtggctg gctttcggaa agtaacaatt agtccttttg
     1261 ttttttctgg atacgtacgg ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     1321 gattacgatc cacaaatagg gtttgaatcg taggtgcgtc aaaacctgtc aatagacgat
     1381 ccactacgat cactaaatca atttgtttcc caaattgttt gaattccgct ctttttcgtg
     1441 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     1501 tataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg acacttgaat
     1561 ctcggctgtt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     1621 tcgttcttcc ttcacgaatg ggctgattag gaaactcttt tgttaaccag tcaggttctt
     1681 tcgtcatttc tttgatcgct cgataatact ttttggccat gtcaatggaa ctcgtggtta
     1741 agatcgcaga cttcgttgga cggccatttc gaaaatcaaa ttttgtatag gcattgtctg
     1801 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagtagggt
     1861 ccaaataact ttcttttttg acaggctcca tttgatcaat catacgattg atctgttccg
     1921 gtgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta ttctctaatg
     1981 aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taaaaccgag ccatcttcta
     2041 atgcattctt gatggtatag gtatgcaaga cctctccata ctgatcatgg gtcgtacgtg
     2101 ccaattgccc tttggcttgt ttccgattct cagggaaaat cggtgtacct gtaaagccaa
     2161 accaagtgga tttcggaaac atttttttga tttcttccat attttcagca cttaacgctc
     2221 gatgacactc atctactaca aacacgatat gttgccccat taatttttgg aagcgattgg
     2281 ttcctttttt ctcttcttgt ttttttgcat atttcaacgc cgcatctagc ttttgacggg
     2341 ttgtaataat cacaacattt gcattggcat ctgccaacag agtttcactt aactctttgg
     2401 cacttcctgt tcccacgatc aagctattcg ctttggcgtt cccggaagaa atcccagtat
     2461 taaattccga agcaaatttc gtaaactctg tggtggtttg attatctaaa tctttgcggt
     2521 ccactaacat gatcgtccga tcgatccccg attttcgtgc taaaagctta gtagatacaa
     2581 aactagtcaa cgtttttcct gaaccagtcg catgccacac atatcctgat tgatgtttat
     2641 ttgcagcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac accatgagga
     2701 ttttattgtc ttgatcttca ctgacaatcg tataatccgc aatcaagcga tgagcatccg
     2761 ggatatttag cacttgtttg caaaactcat ataagttttc taccttttta ttatctttcg
     2821 ttcgccaact aaataaaaac ttaggatgca aatcctttgg taaggcattt gcaaaatacc
     2881 gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata ttgttccgaa
     2941 acataccttc ttctgcatat tttttgattt ggttaaacgc ctgataaaaa ctatcttttg
     3001 cggttacttg tttcagctcg atttggacaa tcggtaatcc attgatcagt aaggtgacgt
     3061 caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga tggaccactt
     3121 cataggtaga gattcctcca ccaatgtctt gattagaata aagaatgagc gagacagaac
     3181 ccagttgcgg atcttcccgt tcaatcgtga tccgagccat tccattttca ccttttaacc
     3241 atttcgcagc atcaaagggg gttttcgttc gtagtaacaa ttctgttttg atcgtttcaa
     3301 attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag atgatttttt
     3361 ggcgtaaatt gacccaaaga tcttcttctg attttagatc gggacgatac gtccactgat
     3421 tatgcccttc ccccaataca cggatcaact gttcttcgac ttccgcttcc tcacggtgac
     3481 taattttcat ttatcttccc tccctaaaca aacatctttt gtaagaagcc ttttttcgtt
     3541 tccttcagca aatctaactt acgttgatga agagcgatcg tgtcgtcgag ttgtttgaag
     3601 aaagcgccga ttttttgttg ttcctcttta ttcctagtaa aaggaaactt gatattagcg
     3661 aaattctcat agaataagta ttctcgtacg cttccttcag tgttgcgctt gacctctttg
     3721 ataaatgttg gtgacttgat aaactgaagt atgaactcat tatctaagtc ctcgctcatt
     3781 ttcacaacta cataaagaga gctaacaatg accgtcatac caagattgtt aaatgcaatt
     3841 gatcctacgt ttactcttgc tggattatag gcgaattcat ttggttcaac gaatttataa
     3901 tttgtttttt ctaaatcatc caacctacta ccatcaaact gttctgtttg agaaattaaa
     3961 cctgctttat tacttaccga ataggcaggg aagtctaaat ctttcgtgtt cttttttcca
     4021 gtcttcttac taaactcttt gaacttacgc tgttcccaat cttccgtaaa tccaggaaac
     4081 cgaatttctg gcacttttgc tccgttttta gggaacattt tttgtaagaa tccttttttc
     4141 gtttccttta ataaatctag cttacgttgt tgaagagcga tagtgtcgtc cagttgtttg
     4201 aagaagttcc caattttctt ttgttcttct aatattggaa attgtactat ccattttttc
     4261 acttctgaca ttggtacgag atttgtaatt gctgaacctg gattagctcc tgtgagtttt
     4321 ctttgcataa aatctgcacg aataaattga ataaaatatt ctgaatctgt gatttcttta
     4381 tttattctaa gtctaatcaa agcctgattg aatacacccg gttttatttg ctttggtact
     4441 cttgaaattc tcccaattgt tccagcgcca ctcatgataa aatcgcctgg ttctaattta
     4501 aatttgatta attcattata ttttttttca gtaattttat atcttgtttc gtaattatca
     4561 taaatagcat tttgttgttc atagacaaca aaattgctat tagatacaaa gaattctttc
     4621 tttaaggcac tcccaaatgg tcccctcctt actccatctt ttttatcaag taagcattca
     4681 aacttacgct gttcccaagc ttccgtaaat cctgggaaac gaatttctgg ttgtatatca
     4741 ttactcatgt ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt caggtgttgt
     4801 ttgcagggaa gagatagctt ctaaaagttc tttttctagc acttgctgtt cttgtcgtat
     4861 cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt caaacgtatc
     4921 tacatagcgt ggaatattta agttatagtc attttctttg atttcatcaa agtttgccaa
     4981 atgagcgtat ttctcgacat cttttctttc agcataggtt tcgaggattt tttgaatgtt
     5041 ttcttccgat aatttattct gattttttcc tttgacaaat tctcgactag cgtcaatgaa
     5101 taatacgtct cgagtttggc ggttcttttt caatacaatc accgtcgttg gaatcgatgt
     5161 cccgaagaac aagttcgcag gcataccgat cacagcataa atactgccgt cttctagtaa
     5221 tttttgacga atgacccctt ctgcagcgcc acggaataaa actccgtgtg gcaagacgat
     5281 ggccatagtc cctgtttcct tcaaatgata gaaaccatga agaagaaacg caaaatctgc
     5341 tttggatttt ggcgctaatt ttccataacg attaaaccga gaatcgtcta agaaggtcgt
     5401 atctgctgac caattagccg aataaggtgg gttcattact actgcatcaa atgtgtaagg
     5461 ttcatctgtt ggccaatcct tattcaacgt atccccattg cggagattca tttcttcggc
     5521 atctacccca tgtaaaatca agttcatttt tgctagattg tatgtcgttg tatttaattc
     5581 ttgcccatga tatttaacgt tatctgggtg agttaagtag ttacgcacat ttaacatcaa
     5641 tgaacctgaa cccatggttg gatcaaaaac actaaagaat cgtcgttctt tttgatccaa
     5701 cgtcacgatt tgtgccatca tatcagaaac catatgaggc gtgtaaaatt ctccggcttt
     5761 cttccctgct tctgaagcaa attgactgat cagaaattca taagcatccc cgatcacgtc
     5821 cccatcgtga cctaaaactt ccacgtcatt caatttctta atgacttcgg taatcgtcac
     5881 atttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat catcaaataa
     5941 cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta gatcattcaa
     6001 ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact ctggcgaaat
     6061 cgcatacccc aaaatatcaa cgattgttgc aatcagatct tcttttgatt cttcatctga
     6121 aagtaattcg cgatacaaca tcgtttgttt gcttaccgta tcatattcct ctaaggattc
     6181 atctgctaaa agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag
     6241 tcccaataaa taatttttat attctgacgc atccattttg ctacggagat tatctgccgc
     6301 actaaataat ttttgattta attctgcacc caaaaagata cactcctatc tcttatttaa
     6361 agttgctata taccctctta ttttagcata ctccctttta gaaaaagcaa aaaaaaaaga
     6421 aagaaactta ttcttgctaa taaactacgc gaaaggagtc taacgttgtt tgatataact
     6481 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
     6541 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
     6601 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
     6661 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
     6721 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
     6781 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
     6841 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
     6901 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatatct ttaccaactt
     6961 tctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
     7021 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagtt
     7081 tccaagtagg tggtatagct gtgtatgttt ctacaaatga attaagtttt tcattaagtg
     7141 acttctcttt tatatttcca tctaaaaact ctgtatatat ggcttgagct atactaaaac
     7201 aaagctggcc ttgttctaat aattttacat cattagttag taaagctgtg ttcacgttat
     7261 caatcgcttc aagaagagta tctttggtgt ccctaacagc tttaggtgtg ccttctggaa
     7321 tttccctact tgatacctcg tccattttat cttccataaa ccccttaaaa tcatatcttt
     7381 ctcccaaatc catagaagtt tttgagaaag ctttcatttc tgagtctatt tcttcaataa
     7441 tatttaacat gtctgataca tattgtttag gctgattcgt ttttaatcta tatgtggtaa
     7501 atatctcaat tcctccaatt atcaataaaa ttgccaaaca aatccataat aattttttct
     7561 ttttcatttt tcctcctata cactgctgtt ataacagtga ctaatgtaaa gattttccca
     7621 gcaatcatca gactattatt tacagggaat caaaaaaact ccttagtaag cttacacctt
     7681 taggagttcc tgtttgaacg gtatgcataa tacatttaaa tcaatttaac attaacacct
     7741 cagtatatgg tatctctaca tatacacggt ctttttttgg atagtactcg acatttatat
     7801 tgatagcact tccattgtct aacgtagctt tagcccggta cgtgttatca ccattatcat
     7861 gagtgatcgt cacatcatca catgtccgat ttaagttata ctgttcttgt aaaatctgag
     7921 ttaccaatgg tacagatgac ttctctagtc cagatgaacc cgtcattctt gaaaaaatta
     7981 tacctataat agccaataca acgataccta taatgacaaa cttcttattc atatttactt
     8041 tattcaactt tttatctcct atctccaaaa tgattttctc tcatcagtaa ctcattgaca
     8101 tccccaatag tacaccaaag atggctccag ctacccctgc tatcatcaaa atcgtaccca
     8161 tttgttcatc tttctctcga tataagtaac ccataataac agcaacagca ccaaatacca
     8221 caggaataaa tgctaatgat actaccatag aaatccaacc aataatcaaa tacagcttcc
     8281 ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt
     8341 tctttactgt gtctgttccg gttgtctcat ctgtttcttg taatgctccg caaaactggc
     8401 aatacttact ttcatttgtt atttctttac cacattttct acaatacatg tcttctcctc
     8461 ctaaaatcat tcaattgctt attagctaac tgtttacttt attaaataca tcagaaaaca
     8521 gctcatttat tggtacactt aatatctcag aaagcatttt catttgatag ggtttaggaa
     8581 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt tttttggcta
     8641 actcagtttg ggaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
     8701 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
     8761 tataaatact tcaaatgtca ttacaaatac aacaacttgt tgtactctac tgagaagggg
     8821 ggtgtattaa tgaaacagaa gcaatatgca aaggtcatta gagaaaagcg aaaagcactc
     8881 gggtggacac aaaaagaatt agctaaaaaa atcttctcta cacaacaggc agtagcaaga
     8941 tgggaaaatt ctgtaacaga acctaacctc gatagcttaa cagctctttc tagagcccta
     9001 ggaacccctg taagccattt tctagacaat gttgacatag attatgagga agagtttttg
     9061 gctctctatc gctctcttag tactgaagat gcaattcgaa caattgacta tatgaagcta
     9121 ttaaaaaggc aagaaaatga acgcaatcag ctattgaaag attgaaaaat aaataaccca
     9181 ccgaacttta acggtgggtg tattgagatg aaataagatt aagagctatg gataacctgt
     9241 ctagtttaat ataattgggt ctcttctatt ccttactctc cgtccatcat ctcttgttac
     9301 acttgtaaat tgataaatag gaactaattc aactcctgat aatatcaatt cttttattac
     9361 ttcatctatc cctgcccctt tcgctagata tactaaaaaa ctactgcttg ctttcacatt
     9421 gtatagtttc ttaatatcct tagaagctcg ttcaagagtg aatccttgtt taacaaagta
     9481 gcctctgttt tctattaaag catttttcac gctgttatcc ctgacatttc gtaggcgttc
     9541 acaaacagtg ttaataagtt ctttttctgt cattagatat gtcccttgcc acgataagcc
     9601 ctgtaaaact tcatgtgctt catctaaaat caattgtttt tccttgctaa gttctgatcg
     9661 ggaattttga ggaaatactc ttttagcctc tttttcgtta aaacatatct taacccattc
     9721 ataagaaaaa tattgatatg taaaatgaaa tttttccaga agtggaagga tatcttcatt
     9781 caatttttga atcacagaag ttgcattaaa aacgtctaat cgatagacat tgcttctctt
     9841 atgtttaaag tttcctaacg ctttctcttc ctttaaccta tttaggtatt cgagcatctt
     9901 caaaggaaca tcttcatcat ctaatttttt tatcaatcct ataaaagtca taagtgatag
     9961 taacttatta catttatcta tgtcataagt tttaccatag taagaactca tttttttagc
    10021 taattgttcc cccgacaaaa tacaagtcac actttcctta ctatcatcaa aaaaattaac
    10081 atcagaaagc aagacatcat agacacgtcg catttcctct agataattcc ttattgtcct
    10141 gaaaagtttg tctttcattg attcaagagt gtcaaaaagt acttttgatt cctctttcaa
    10201 ttttcgaaaa tgtgtaggat acaagtctag gttctctaat agccaattaa atgtttctac
    10261 ccgactgtct ttctttaaaa ttagcatcac aactccaata aaattttcac tgatatcaca
    10321 attactggaa tgacaataaa aaatggaatg cccagtttta ctggtaaaaa tactagcaga
    10381 gggattatga tcgttgtgaa acagacatct taagttttta cttgacttaa gtaatttatt
    10441 catatcaatg gttaacaatc tttcataaat ctcattaaaa gacatgtcgg aggaatcaaa
    10501 caggcagtct ttaaaccact gtttcatttc ttctttgtcg tcatttttta tcaacttaac
    10561 aaaatcggtt tcattgacta gttcatactc tctccctgaa tcactttgac aatttttaga
    10621 acatgtcatg gtaaaatcaa tcggaagctc agtaatatct aacatgttgt cttcattaat
    10681 tattatcact gcatctgact tatatcctcc aaaaaacatt ctattcgaag catttacatt
    10741 gtcatcacac ccggggatgt tctttttcaa gtattcgtaa acagcaacca ttttaccata
    10801 gtcagtaatt gcttctttta atagaaagac aatcttgtat ttttctagat tttcagagta
    10861 ccgaatactt ttttgtatcg caaacgcaga attatgaact aaattgatag ataaaacctt
    10921 ttctatgctt aagtatctac tatcatcttt actcaaatct actaccttcc ccttctcatc
    10981 tttaacagtg ttatcgacat ccagaataat tactctggtt tccacaaaac tctccttgtc
    11041 attgccttta ttctttgtct tgcttggaat aaatgattgt gatgaattta gtatctctac
    11101 aaactcatct cttgtaactt ctctatagtt atctaaacac ctatctctta catcagtttt
    11161 ttcttttttt gtaactggct tctctaataa tttttcttga tctacataaa ttggaatatt
    11221 aatcattgat gtttccattt gtgtttcctc cttataaaat tctgttaaag actaaaacat
    11281 acagataata aaaacaaact ctttattact atatgtttta gtcttatttg tttactcaac
    11341 ctttataccg ttccatgaaa ctatcaatat cagatttttt aaatctaatg accgtatcaa
    11401 tttttataac aggtaacccg tattgagaaa catattttct aatcgttcct actgatacac
    11461 ctagatattc acttgcctgc tgatagtcca tatactctag agtgactcca gaaataatgt
    11521 cttctagcat aggctttagc actgtaatta tctctttcac ttgttgttca acttgtatag
    11581 aaacaatgct atcaatgatt tgtagagaca tatttccccc tcctttcgtg attgcttgaa
    11641 caaattttct aagaattatc ttcgtatttt ttgctcaaaa taaaagtatt ttgttattaa
    11701 ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca tacacataat acctcgataa
    11761 aaaaggggtg tcaacagctt tgtaaaattt atttacaaca cccctttttt gaggtatact
    11821 aattttagga ggtgtggtca atatgataaa acgtccacca ataaattatt tagaacgaaa
    11881 aaagatactc ggaacaaaaa taaaagctat aagaaagagt aaaaaattaa cacaaccagc
    11941 atttggtctt atgattaaca acggtcaatt aattgacaaa aaaactatct acgaatggga
    12001 gaagggaacc tatttaccaa taccagagcg attatcccga attgctgatt tgggaaatat
    12061 gagtatagag gagttagttt gtggcaatgt agaagaatat attttgggaa ttattcttta
    12121 tagagatagc attgtactag atggtataac atttccagat aaaaacctgt tccagcatct
    12181 tcgtcaacag ttcccaccag tacactctaa tttagataca tggctggata gatattccaa
    12241 attagaacca gagatgcaag aatttattgc taacaagact tgtaataaag ttaaaaatga
    12301 aaaaattagt cttttcaata tcttaaaaat agaagaatta ttcatcaatg ctattgtaga
    12361 agaatttgac aataacattc ttttcctcac tagcagtata gaagaactac tagaaagaat
    12421 ggtagatgaa tggctaccta ttcaattgaa agacatgtct tatcccgaag aagctgtgag
    12481 agaaattact gataatataa acaagctaga acaaacgatt tcatcaattg ggaaaaaata
    12541 cactaagaaa aaaatgaagg gaggtgatac catctagtgg taagtactcc ttgtaaacta
    12601 agaggagatg atttaatggc aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg
    12661 tggatgtata aaggacacat tgccaccgat ccacgaactg gtgaaaaaat aaatactacg
    12721 agaagaggtt ttagtagcaa acaagaagct caaaaagatt atgacgagta cagacatcaa
    12781 atattatacg gtgttaaaaa gaaagcacct gatatgacat ttgaagacct gtataatgaa
    12841 tggataactc atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac
    12901 gcaaataatc aaatacttcc agcatttggt aagcttaaac tatcgaatat ctctgtccca
    12961 tattgtcaaa aagtggtaga tgaatggcac agtaaatatg aaagttacga ttatatgaga
    13021 aaacagactg cacaaatttt acgctacggg gttgcaatgc aatatattga caataatcct
    13081 atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa attttactca
    13141 aaagaggagc ttaataattt actggatgct tttaaagatt ttggtaacat gaaacaatac
    13201 gccttcttca gattattggc atacacaggt atgagaaaga gtgaagtact cgctttacag
    13261 tggaaagaca tagacacgtt taataaagaa ctacatgtca ataaaactct tgctgtagat
    13321 gaatttggta aagttataat tcaatctccg aaaacaagag cttctagacg agttatctct
    13381 ttagatactg agactctttc tatcctcaac aactggaaat tacagcaaaa agaagagtat
    13441 ttaaaacttg gatacaatac tagctctaaa gagcaacatg tttttacaac agttaaaaac
    13501 actctataca ttccaaacac tgttaatgat tggttaagat atattttgaa aaaatataac
    13561 ttgcctcgta ttacacctca tgggtttaga catacacatg ctagtttatt attagaagca
    13621 ggagaatctg tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa agtgacatta
    13681 gacatctatg cacatattac aaataatgca cctaagaaaa caggtcaaga ctttgctgat
    13741 atgatggctc atcagtaaaa gagctggtca aaatctggtc atttctagta tacaggcaaa
    13801 acaaaaaagc tagaaccctt atatatcaag ggttctagct cctataacag gtttacccaa
    13861 cactgccttc catctcataa ctgatcaatc gattgagttc gactgcatat tccattggaa
    13921 gttctttggt gaatggttca acaaagccca tgacgatcat ttcagttgct tcagattcgg
    13981 tcaggcctcg gctcatcaga tagtagagtt gttcttctga gatttttgag actttggctt
    14041 cgtgttctaa agagacttgg ctgttgtgga tctcattgaa tgggatcgta tcggatttcg
    14101 accattcgtc catgatgatc gtgtcacatt cgatatgaga gatcgaaccc gcactgtttt
    14161 tggcaaaggt tacttgtcct cgatagttta cttctccgcc gtcttttgaa attgatttag
    14221 agacgataga gctagatgta tttggtgcat tgtggatcat ttttgcaccc gtatcttgga
    14281 tttggtttgc tcctgcaaag gcgatggaga gcatggtgcc tcgtgcacct tttccatcta
    14341 agtaaacact tgggtatttc atcgttgttt ttgcacctag gtttccgtcg atccattcaa
    14401 cggttgcgcc ttcgtatgct tttgctcgtt tggttactaa gttgtacaca ttgtctgacc
    14461 agttttggat cgttgtgtaa cggcaatacg catcttttct tgtaaagatt tctacgatgg
    14521 cggcatgcag gctgttactt gaataagtag gtgccgtaca accttcgaca tagtggatgc
    14581 ttgctccttc gtcaacaatg atcagcgtac gttcgaattg ccccgtgttt tccgcattga
    14641 tccggaagta cgtttgtaat ggaacatcga ctttgacgcc ttttggcacg taaataaagg
    14701 tcccacctga ccatacggct gaattcagcg ctgctaattt gttgtcggtt ggcggtacaa
    14761 gttttgaaaa gtactctttg aataattcag ggtattcttt caaagcagag tcagtgtcag
    14821 taaagacgat tcctaacttt tcgaattcat ctttcatatt atggtacacg acttctgatt
    14881 catattgtgc ggaagcaccg gctagataag ctcgttcagc ttctggtata ccaatctttt
    14941 caaatgtatc tttgattttt tctggtacgt cttcccagtc acgagcgggt ttgtcacttg
    15001 gtttttggta atatttgatg gcatcgaaat cgatgtctga aaggtcgggg ccccatgttt
    15061 gcattggcat tttgttaaat gtttctagag atttcagacg gaattcaagc atccattctg
    15121 gttcgccttt gattcttgac atctcacgga tcacttcttc tgtcaagcct tctcctgtac
    15181 tgaaaaccgg ttcgacatca tcgtgaaacc cgaatttata ttcttctaat tctggtacgc
    15241 ccatgttctc actcctcttc cttgtggtaa tgatggaaag ctgcggtacc atcgttttcc
    15301 attgcttttc ctagtgcttt ccaggcaagt gtcgcacatt tgatccgagc gggaaattta
    15361 gctacaccac caagcatagc agcatcgcct aattggtcgg gatctttgac ttcttttcct
    15421 tggacaagct gtagaaagtc tacagttaaa tgttctgctt cttctattga ttttccgatg
    15481 acggcgtctg tcatcatcga ggcacttgcc gtactgatgg agcagccact gccgtcaaag
    15541 gcgatatttt tgatgattcc gtcttttaca tcgacttgca attcgatcac atctccacat
    15601 gtggggttgt tcaattcgat cttgcttgtc gagtcagtca agcttccacg atgatgcgga
    15661 tgggaagaat gatccaagat cacttgacgg tataaattgt ctaatttaga tagtcccatg
    15721 ttggaaaaac tcctttgttg ccatgattgc ttcaactaaa cggtcggcgt cttgcgttgt
    15781 gttgtagaaa taaaagctcg cgcgagcagt cgctgatact tcaaggtact tcaatagcgg
    15841 ttgcgcacaa tgatgacctg ctctgacggc taccccttcc atgtctaaag ctgtcgcgac
    15901 atcatgggga tgtaacccat ccaaattgaa tgcaacgact cctgtccgaa aattcgggtc
    15961 ttgtggacca taaacagtca atccatcaat tgcttgtaat tttggcaaca cataagcggc
    16021 tagttcttct tcatatcgat ggatattgtc cataccgagt ttttgcaaat aatcgattgc
    16081 atggcctaat gcgatagcgc cggcgatatt tggtgtccct gcttcgaatt tccaaggtag
    16141 ttctttccat gtactgtcgt ataattcgac aaaatcgatc atttcgccac caaattcaac
    16201 aggttccatt tcttctaacc attttcgttt accatataaa acaccaatac ctgttggtcc
    16261 gcacattttg tgtccgctga aagcaaagaa atcacagtct agtgcttgga catcaatggc
    16321 catgtgcggg gcagattgag caccatctac tacgatgacc ccgccgtttt catggacaag
    16381 ttcagctaat tcttgaacag gattgaagac acctaagaca ttggatacat gagcaatgga
    16441 gacgattttg gttttgtccg taatcttttt cgctgcatcc tccatatcca ggaagccttc
    16501 ttctgttagt tcgatatatt tcaagacagc acctgttcgt tttgccaact gctgccacgg
    16561 gataacatta gaatgatgtt ccatgtaaga gagcactatc tcgtctcctg ctttgatata
    16621 gcggtcgccg aaggttctgg ctacccaatt caagcttgtc gtcgtaccac gagtaaacag
    16681 cacttctgct gtttcttttg catggatgaa cgcccgtact ttttctcttg cttgttcata
    16741 atcatgggtc gcgcgttctg ctaacgtatg gacaccgcga tgaacattgg cgttatcgtg
    16801 ttcgtaatag tacgctaatg cctggagtac ttgttttggc ttttgggtcg tcgctgcatt
    16861 atccagataa actaaaggtt catcattgac gtcttgaaat aagataggaa aatcattgcg
    16921 tagcttttta gcatcgatca tccattcaac ttcccttcga ttacttcgac taattctttc
    16981 cgaacttgtt ctacagggat tgctgttaag acagaaccga ggaatcctcg aatcactaag
    17041 cgctctgctt cttctttatg caatcctcta ctcatcagat agtacatttc ttctggatca
    17101 actcgtccta cactggcagc atgtccagca gttacttcgt tttcatcgat caaaagaata
    17161 ggattcgcat caccacgtgc tttatcagaa agcatcaaga cacgactttc ttgttgggca
    17221 tctgcacctt ttgccccttt caagatgtgg ccgataccat taaaggtcaa tgttcctttt
    17281 tctcggatca ctccgtgttg caaaatatgc ccgatcgtat gaggggcttt attggttact
    17341 cgtgtatcga ttccttggat ttgtctgccc gagctgattg ccacgatttt gacttctgcg
    17401 tgtgcacctt ctcctgctaa atctgagtca aagtcagcga tcacatgacc gtcattcatg
    17461 acaccaattg cccagtcgac agaagcatct cgtaaaatgt gcccacggcg attcatataa
    17521 gaagtaatat tttctcctaa ttgatctacg gctgaatatt tgatttttga accagcttta
    17581 gcgatgactt ccacgatgat attacctgat gattttgcta cttgttcttt ggttgtctgg
    17641 aaacgttcca gataagaaaa ttcactatgt tcatctgcca cgatcaacac atgcttgaag
    17701 aagtgctcat cggatgcgcc atcttgaatg aataaggact cgagtggttc atcgatcacg
    17761 acatttttag gcacatacag gaaaataccg ctgttcataa aagctacatg gagtgcagtc
    17821 aattggtctt cgttcatgtt tactgctttt ttcatataat attcttggac tagttctgga
    17881 tattcgatca ttgcagtgaa caagtcggta aaaatgactc ctttttctgc taattccaca
    17941 ggcagttgtt caaaaatcgt ccatgaacct tgttggacaa gcacaggatt gtctttcatt
    18001 gcatcaaatg ccggaatcgt gcctgtttca ctgatggttt gtgtttcttt cacatcaaat
    18061 aacgaccagc gatggaattt cacccgatcg atatgcggca atggcagttc atctgctttc
    18121 gctagtgcgt cttgacggaa ggttctcatc caatcaggtt cgcctttact taaagaaaaa
    18181 tcatttaccg cgtcaagata atcgagccag tttttttctt tcattcttgc tcccccttac
    18241 gcttcttctt ctttatagtc gattcctaat tctttactga taccagcgta gccttctgct
    18301 tccagacgtt tagctaggtc agcattccct gtcatgacta ctcggccttc catcatgatg
    18361 tgaacgacat caggtgtgat atagttcaat aatcgttgat agtgggtgat gatcaatgca
    18421 ccgaaattgt ctccgcgcat ttcatttact cctttagcaa cgactttcaa agcatcgata
    18481 tccagacctg aatcgatttc gtctaaaatt gcaaatgtag gttctaacat caacaattgc
    18541 aggatctcat tccgtttctt ttctccgcca gaaaaacctt cgttcagata acgttcagcc
    18601 atttcttctg gcatatttag taaagccatt ttttcatcta actttttcaa aaatgccata
    18661 acagaaattt tgtcgttttc atcacgtttc gcattgatcg ctgcacgcat gaattccgca
    18721 ttcgtgatac ctggtatctc acttggatat tgcatagcta aaaataagcc aagtcgcgca
    18781 cgttcatcta cttccaagtc taaaacattt ttcccgtcaa acaaaatttc gccttctgtt
    18841 acttcatagt ttggattgcc catgatagcg gcagataagg tagattttcc tgtgccgtta
    18901 ggtcccatga tggcatggat ttctccggtt ttcattgtta ggtttacgcc tttaaggatt
    18961 tctttttcct cgatcgacac atgtagattt tttatttcta agacagacat gcattatacc
    19021 cccaaattct ctttcgttct cactcatttt agactaattg agaatgaagc gcaatgacct
    19081 tccttactaa aatcaaatat cttttattta ttattaaaca attttgattt ctaaattaga
    19141 ataggtataa aaaagatgag aaataagcca ttacagactt atttctcatc ttttttgttc
    19201 cataccttat tttgcatcag cctttactgg tttaacagag ccgccccatt tgtctgtgat
    19261 ccattcttgg acttctttgc ggtgaagcac ttcaatcagt tttttgattt tgtcactgtc
    19321 ttcatcacct ttacgagtcg caataatatt cacgtaagga gagttgtcct tttcaagcaa
    19381 tacggcatct tctaatggat tcaaccctgc gccgtaagcg aagtttgcgt tgattgcaac
    19441 taggtcacct tcatcatttt ggtacgtaga agctaaaagt gatgggtcga tcgtatggtt
    19501 gaacttcaag ttcttagggt tttcttcaat atcatcgaat gttgctgttt ccagatctac
    19561 tccatctttg actttgacta agccagcatc ttgtagaatt gtgatgatgc gtccccagtc
    19621 tgattcagaa tttgaagtaa tgattgttgc accatctttt aattcggaaa catctttgac
    19681 ttttttagag tacaagccca ttggttcgat gtggacagca ccggcattga caaagtcata
    19741 tcctttttct ttgatttctt tgttcaaata aggaatatgc tggaagtaat tggcatcaat
    19801 gtcgccttct tccaaagatt ggttcggtaa tacatagtca tcgaatttga cgatttcaag
    19861 atcgattcct tcatctttca ataatggttt cacatgttcc aaaatctctg cgtgcggtgt
    19921 cggtgaagcg ccgactttca atgtcgtgtc gtctgcttta tttgctgaat ctttgctgtc
    19981 gctgttgtta ccgcatgctg ctaatcctac agtcagcaaa agcgctgctg caaatccaaa
    20041 aattttcttt ttcatatcct acatccccct atatcctatt atcttttgtc tgttttctta
    20101 gttaagaaat caccgattcc ttgaacgata aatacgatta gtaaaataat taaagtcgca
    20161 acgaatgtca ctgttagatt accacgctgg tagccttcct gccaagcaag acctccaagt
    20221 ccacctgcgc cgatcgcgcc ggccatcgca gtaaatccga tcatcgagat cgttgtaact
    20281 gttaatccag aaatcaatgc gggaatactt tcaggtatca agaccttcca gatgatttcc
    20341 cagtaacttg ctcccattgc atctgctgct tccagtacac cagggctgac ttcacgaaaa
    20401 gcaatctcta ctagacgggc ataaaatggt gctgcggata aaacaagagc tggaatggct
    20461 gcttgcgcac ctagcatagt gcctacgatt gctttagtaa atggaatgat caaaaccatc
    20521 aggatcataa atggcgttga ccggaaaata ttactgatga tcgatacgat tccataaatg
    20581 attcgagcat acggtttgtt tttgcgtcca atagagtaaa ggatcaatcc caaaactaat
    20641 ccgatgatcg tcactaagac cattgagatt aatgtcatat atagtgtatc aaggatagcc
    20701 tgattcatcg tttccgggtt gatttgttta aaatctagat atgtttctga aaagctttta
    20761 tccacgatgg atcacctcaa ttcctactcc ttgttctttg atgaacactt ctgcttgatc
    20821 taattctttg gtttcaccag tcatcaagac agtcaatgaa ccaaaggaac cttctttcac
    20881 ttgtttgatc tttccataca caacgttgac atcgatgtta tagcgtcgaa tcgcttggga
    20941 aatgactggc tcgttggcat ttctttcgct aaaggtcaaa gttgccaaac ggccgcttgg
    21001 attttctttg atgagttcag ctaatagttc tgtcgaatca gcttctggtt cgatgtcttg
    21061 ttgtacaaag cgtttggtta cttctttttt cggttgtctg aagacagtca atacgtctcc
    21121 ttcttctacg actttcccca actccatcac agcgactttg ttgcagattt tgcggatcac
    21181 attcatttca tgtgtgatca agacgatggt caaattcaat ttcttattga tcgctaacaa
    21241 taagtctaat acttcttctg ttgtttgcgg gtccaatgcg ctagtagcct catcacaaag
    21301 caggatctca ggatcatttg ccaatgctcg tgcgataccg acgcgttgtt tctgtccgcc
    21361 ggaaagttgt gaaggatatg cttgtccgcg tccttctaac ccgactaaac gcaacagttc
    21421 ttctgcccgt tctttacgtt tgctctttgg tacgccagct aattccagcg gcagctggat
    21481 attttcaagt acagttcttg accataacag gttgaaatgc tggaagatca taccgatttt
    21541 ctttcggaat gtacgtaatt ctttgttttt taattttgta atatcttgat cgtttacgat
    21601 cacttcgccg ctcgtgggaa gctcaagtcc attcaggaga cggactaatg tactttttcc
    21661 tgcgccagaa tagccgacga ttccataaat atcccctttt tcaacgttta gtgaaacgtc
    21721 attgacggct tggatgtttc cgtttttccc attgaatgtc tttttgacat tcgttagttg
    21781 gattaatgac atgccattca catctttctc ttatgatttt tcaaacaaaa aagccttcgc
    21841 cctcttgcct taagcaaaag gacgaaagct tgaacttccg tgttaccacc ttgatttacc
    21901 gttacttcgc aataacagcc tcttccagta cttggactta gcttctttcc gtatactgtc
    21961 gcgctgtaac gggcactccc gtagtaagtt tgcttacgct cgcttactct gctctaagac
    22021 catcttccat ctcattttcg ttacccattt tcagcaaaca gggctctctt caaacgtccg
    22081 tgacatgtac tcttcttttc aaagctttct cgtttcatat aggattgctt atgattatag
    22141 caactggaag aatcagtgtc aacttttttc aagtgatttt ctgattttat aattcgtcaa
    22201 attcttttgg atcaacatct ttgaaactta agatccacgc attcatttca tctgaatcat
    22261 tcaagatatt gatatcttca ccgattttat cattaacaga agaaactgta cccgttagcg
    22321 ggctggcaaa ttcattgacc gctttttccg cttcaacttc gatcagaggt tcatcttttt
    22381 ttagcgattg tcctgctttt ggcagattgg caaacgtgat gtctccaagt tcttcttgcg
    22441 cttcatttgt caaaccaaca cagtattctt ttccattaaa taagatccaa aggttgtcct
    22501 ttttcttcaa atgcttgcct gccattttat atcagttctc cttcataaat tttttcattg
    22561 aatccaattg caacaagctg gtcctctcgt aagatcagtg gtcgcttgat cagcatacca
    22621 tctgtcgata agacttggct cgcttttgaa gccgtaaact cgcctacttg atctttcagt
    22681 ccaagttccc gatatttcat cccgcttgtg ttgaacaggc gacggattgg tacttgactc
    22741 gtttctagcc aagtctcaat cgttgaggcc ttcggtactt ctttgatcat atcaacggtt
    22801 tgataactaa cgccatgctg gtctaaccac gctttggctt ttttgcatgt tgaacatttc
    22861 gggtaccagt aaaaggtgta caattttctt acctccttga agatcttgca ggtgcttcat
    22921 tttggtatct catcgataat atcaacccga taccgatcat gttccctaaa atcgaggatc
    22981 ctccctgact aatgaaaggc aatgggatcc ctgttaaagg aagtaatccg atatttgctc
    23041 caatattctc aaagacatgg aacagcagca tcatgatcaa accgcttgcg atataggcgt
    23101 aaaattcatt attcgtatcg aaacacaccc gaatcatacg atagattaaa ataaaataca
    23161 agaaaatgac gaatgcacca ccgataaagc cgaagttttc accgataacg gtaaaaatca
    23221 tatcggactc acgaacaggc acatagatat tgcttacgtt gaatccttta ccaaacaagc
    23281 caccggtacc aattgccaaa agtccttggg ccggctgata actcattcct gacgtatcat
    23341 ggaaagggtc cagccatgaa tcaatacgtg caaactgata agatttgaat ccaactttat
    23401 aaagcaagtc tcttccgcct tcagtcgtaa ccaagaagat cgttcctgct ccaacgacta
    23461 ctgccaagat tactacaggt gcgatgatgc gccaagagat tccagacatc aggaaaatcc
    23521 cgccaaagat cgcaagaaag acaagcatcg ttccaaagtc atcttgggcc atgatcaatg
    23581 ctaaaaccgg tattgtcacg atcaagagct tcccaatcag ccaaaagtcc gatttcatcg
    23641 tccgttcttt tacttgcgta ttatgtttag ttacgatcag tgccatcatc aagatgtacg
    23701 caatcttcat caactctgct ggctggaaag taaacgcacc gaatcgaaac cagtttttag
    23761 accctgttga agcagctaaa ttctgatcat agaatttgag caagagtccc attaccacta
    23821 accccaaacc ataaaggtaa ggagtcagtt tccagatcca tttggaattc aaatgcatga
    23881 tgacaacgat tgctgccgct ccaactaaat accagacaac ttgcatccct acgcctttca
    23941 agactgctgg attgttcgga tcatggctaa gtgctacata taatgagagc agaccaatca
    24001 aacataacag aaagactggt aaaatcacgc cataatcaat acgattatcg ttcgaagctt
    24061 ttaatttttt ttccatgttt catccctttt cttaaaaacc atactgctga ttattatagc
    24121 ggtccagtta gataatggaa agtctttcca tagacatttg ggaaaaatta actttccgtt
    24181 tgtgcagttg cttgagcaaa tgcagtacaa aagcctactg cttatcttta gtgtatcata
    24241 gaactttttt tgatacatgc aattatctac cagctgagat tctcctcgct gtgagaagtt
    24301 tctgtgcttt cattgtatcg aaggatctct tcggcaatct ctagcagctg atcagaaacc
    24361 agattggttc gttggatgaa ataaaaatat cgatggaaat tcaacggagt cattggcaca
    24421 ttttctggaa cagagcgctt agacaaaatc gagcgaccga ttccttcctt cagcaacgca
    24481 acaattacat cgttactttt cacgatcatc ttcttatctt ggatattcat ctcttcaaaa
    24541 taacgctgcg tgtaatgaaa aacaccagag tttgcttctc ttacaagcca caatttgctt
    24601 tttgggtccc cagcgatgac tagctggtct tccatcaaag agtatctttg tacgccggaa
    24661 gtctctaatg gcttttcgat aaaaccaaaa tcggattcat gtttcgctac ttgttctaat
    24721 gattcatgag aattcgtgat ttcgatctca aaatgcactt taggaaacct tttgatcaga
    24781 tgtttcatca actgaggcaa aagatagaca gcaaaggtgt gggaagcaac gatccgacat
    24841 acttcttctt cattttgttc attgagcaat ttttgctgag tttcttccca ttcttctaac
    24901 aaaaccagtg tccgttcata taataagtcg gcttgcttag ttggaagcat ctcttttctt
    24961 ccgttacgtt gaaaaagatc gatattcaat tcccgctcca actgtttgat ttgattcgag
    25021 actgctggtt gagataaaaa cagtacttct gctgctctgg aaaaatttct tgtttcatat
    25081 actgctcgaa aagtcgttaa taacttaaac atggcaccac ttccataact ttttatgata
    25141 attactataa aaactatttg atttaaatat acaaaaaacc atctataata gaaatgtaca
    25201 acaaaacagt tgctgatcca actagtaact tagatgaaga caatcagctg tctgcttgct
    25261 cgtaatcata cattcctatt cattttacaa tgtattcgtt catcttggta ctaaaatcta
    25321 ttaaataaaa aggagaatat tatatatgaa gaactttttg aaaattattc cgatcccgat
    25381 ctgcgggttg atccttgggt tagcttccct tggaaatctg tttaaagact atcaccatgt
    25441 tgctttaggc aatctaactg gcggtatctc tatgattctt atgatattca tttttgttaa
    25501 attgtttatc ttattcgaac acacgaaaca aacgttgcat gatccaatca tcgcctccgt
    25561 ttcaccaact tttacgatgt cactcatggt tatttgtact tattttgttt catatgaaag
    25621 tattgcacca attgtcaaat tcatttggct gtgcgctgtt atcttccaag tgatccttgt
    25681 cttttatttc aattatcacc atgttatcaa agctgatctt tcaattgaag caatttatcc
    25741 aagctggttt atcatttacg ttggatttgg ggtcatcact gtaactgccg ggaacttctt
    25801 cccacttatt ggaaaaattt tcttttggat ttcattgatc tgctatgcgt tcttattgcc
    25861 gatcatcatc aaacgtgtct ttttcgtaag aaatatggct catccgacac gtccgctgat
    25921 tacgatcgtt gcagcacctg gttctctttg cctaacaggt tatcttaaaa actttgctga
    25981 acctaatttt tatcttgtcg tagcattgtt cgctttatct caagtattgt atttcgttgt
    26041 gctgtctatg cttccacgtt tgttcaagct tcgtttttat ccaagctatg cagcctttac
    26101 attcccgctt gttatctctg caacagctat atttactact gttcattact tcaacagttt
    26161 aggtatctat agtagcgtat tggatttctt aaaagttgcg gaatgtctgt ttgcaagtgt
    26221 agttgtcttc tacgttcttg ctcattacgt acgtttctta attaaagaac atagcaaaca
    26281 tggaaaattt gtttctaaaa aaatcgctta ataatcgatt caataggttg tgaaaaagct
    26341 gttctgcatc aagtaataag aacggccaaa caaaaacagc tgttcatgtt tttgta
//
LOCUS       DACEND010000027        26166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEND010000027 DACEND010000000
VERSION     DACEND010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.028"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..825)
                     /locus_tag="IW746_000954"
     CDS             complement(130..825)
                     /locus_tag="IW746_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1077871.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW746_000955"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW746_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1077872.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IW746_000956"
     CDS             complement(2024..2215)
                     /locus_tag="IW746_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1077873.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IW746_000957"
     CDS             complement(2236..3126)
                     /locus_tag="IW746_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1077874.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IW746_000958"
     CDS             complement(3113..3880)
                     /locus_tag="IW746_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1077875.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW746_000959"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW746_000959"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1077876.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW746_000960"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW746_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1077877.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW746_000961"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW746_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1077878.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IW746_000962"
     CDS             8496..8834
                     /locus_tag="IW746_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077879.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IW746_000963"
     CDS             complement(9002..9760)
                     /locus_tag="IW746_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1077880.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IW746_000964"
     CDS             complement(9778..10596)
                     /locus_tag="IW746_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1077881.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW746_000965"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW746_000965"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1077882.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW746_000966"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW746_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1077883.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IW746_000967"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IW746_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1077884.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IW746_000968"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IW746_000968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1077885.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IW746_000969"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IW746_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1077886.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IW746_000970"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IW746_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1077887.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IW746_000971"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IW746_000971"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1077888.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IW746_000972"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IW746_000972"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1077889.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IW746_000973"
     CDS             20638..20937
                     /locus_tag="IW746_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1077890.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IW746_000974"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IW746_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1077891.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IW746_000975"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IW746_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1077892.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IW746_000976"
     CDS             21904..23877
                     /locus_tag="IW746_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1077893.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IW746_000977"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IW746_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1077894.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IW746_000978"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IW746_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1077895.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7938 a         5153 c         5191 g         7884 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacg
//
LOCUS       DACEND010000028        25776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEND010000028 DACEND010000000
VERSION     DACEND010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.029"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1096
                     /locus_tag="IW746_000979"
     CDS             <1..1096
                     /locus_tag="IW746_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1077896.1"
                     /translation="LEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLARSVKL
                     HYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANLFLIF
                     YLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAAVPFK
                     KGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAGYQMT
                     HSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLLFSLC
                     MRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYGGTSY
                     LILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1188..1943
                     /locus_tag="IW746_000980"
     CDS             1188..1943
                     /locus_tag="IW746_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1077897.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2001..4040
                     /locus_tag="IW746_000981"
     CDS             2001..4040
                     /locus_tag="IW746_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1077898.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4149..5300)
                     /locus_tag="IW746_000982"
     CDS             complement(4149..5300)
                     /locus_tag="IW746_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1077899.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5454..5984)
                     /locus_tag="IW746_000983"
     CDS             complement(5454..5984)
                     /locus_tag="IW746_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077900.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6083..6433)
                     /locus_tag="IW746_000984"
     CDS             complement(6083..6433)
                     /locus_tag="IW746_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1077901.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6590..8119)
                     /gene="ahpF"
                     /locus_tag="IW746_000985"
     CDS             complement(6590..8119)
                     /gene="ahpF"
                     /locus_tag="IW746_000985"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1077902.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8129..8692)
                     /gene="ahpC"
                     /locus_tag="IW746_000986"
     CDS             complement(8129..8692)
                     /gene="ahpC"
                     /locus_tag="IW746_000986"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1077903.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(8836..9498)
                     /locus_tag="IW746_000987"
     CDS             complement(8836..9498)
                     /locus_tag="IW746_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1077904.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            9682..11328
                     /locus_tag="IW746_000988"
     CDS             9682..11328
                     /locus_tag="IW746_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1077905.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11319..12401
                     /gene="uxuA"
                     /locus_tag="IW746_000989"
     CDS             11319..12401
                     /gene="uxuA"
                     /locus_tag="IW746_000989"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1077906.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12509..14062)
                     /locus_tag="IW746_000990"
                     /pseudo
     CDS             complement(12509..14062)
                     /locus_tag="IW746_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14210..15351
                     /locus_tag="IW746_000991"
                     /pseudo
     CDS             14210..15351
                     /locus_tag="IW746_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15477..16556)
                     /locus_tag="IW746_000992"
     CDS             complement(15477..16556)
                     /locus_tag="IW746_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1077907.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(16659..17099)
                     /locus_tag="IW746_000993"
     CDS             complement(16659..17099)
                     /locus_tag="IW746_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1077908.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17156..17566)
                     /locus_tag="IW746_000994"
     CDS             complement(17156..17566)
                     /locus_tag="IW746_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1077909.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(17746..18180)
                     /locus_tag="IW746_000995"
     CDS             complement(17746..18180)
                     /locus_tag="IW746_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1077910.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18280..19128)
                     /locus_tag="IW746_000996"
     CDS             complement(18280..19128)
                     /locus_tag="IW746_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1077911.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19517..21283
                     /locus_tag="IW746_000997"
     CDS             19517..21283
                     /locus_tag="IW746_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1077912.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21385..22644
                     /locus_tag="IW746_000998"
     CDS             21385..22644
                     /locus_tag="IW746_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1077913.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            22791..24092
                     /locus_tag="IW746_000999"
     CDS             22791..24092
                     /locus_tag="IW746_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077914.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24095..24946
                     /locus_tag="IW746_001000"
     CDS             24095..24946
                     /locus_tag="IW746_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077915.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24959..25774
                     /locus_tag="IW746_001001"
     CDS             24959..25774
                     /locus_tag="IW746_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1077916.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         8619 a         4692 c         4817 g         7648 t
ORIGIN      
        1 tttagaagta tacagtgcca gttcttatcg tttattgcaa gccgatgaaa atacaaaaag
       61 tctattattg cgtcaactaa ttttcatatt tttgagttgg agcgtgatct tcttagctcg
      121 ttcagtcaaa ctacactatt tacttcaccc taaaatagca ggatacggtt tagccttatc
      181 gattttcttt ttagtattag taagaatagg gatattcggt gtcactgtca acggcgcaca
      241 acgttggatc tctctgtttg gcattcaatt ccagccttct gaactggcaa atcttttttt
      301 gattttttat ttaagctggt tttttcgtga cggaaatagt agcccaaaag atctaaaaaa
      361 accattcctg attacagtag gtataacttt tctgatttta tttcagccaa agattgctgg
      421 agcattgatg atcctttcga ttgcgtgggt catattttgg gcagcggcgg ttccatttaa
      481 aaaagggatc tatctaatcg ttactttttc tgcattgctg attggagcag caggcggggt
      541 attatattta ggaaataaag gttggcttcc acagatgttt aatcatgctt atgaaaggat
      601 cgcgacttta agagattctt ttatcgatag tcatggtgcg ggctatcaaa tgacccattc
      661 gttttatgca ctatataatg gtggcatatt tgggcgggga ttaggaaata gtatcacaaa
      721 aaaagggtat cttccagaga cagaaacaga ttttattttt tcgattatta cagaagagct
      781 tgggctaatc ggtgcattgt gtgtgctttt cttattgttc tcattatgca tgagaatctt
      841 ttgtttaagc agtcgctgta aaaatcagca agctggattg tttcttttag gattcggaac
      901 tttgctattc gttcagacga taatgaacgt gggaagtatc gcggggctga tgccaatgac
      961 tggtgtgcca ttgccatttg ttagctacgg aggaacaagt tatctgatcc tttctttagg
     1021 aatcggaatc acgttgaata tttcttcaaa gatacaggcc gaagagttgg cgctctatcg
     1081 acctgaaaaa caataaagag aaactgagaa actctcagtt tgaattatca taaagtacaa
     1141 tcttttattt ttcaagcgag aggttctttt agaaagagga attcgcaatg aaagtatctc
     1201 aaaaaatcat attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg
     1261 agtttatcca tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga
     1321 aaagtatccc taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg
     1381 aaaacggtcg ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa
     1441 aactgatctc tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga
     1501 taaatgcagc ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt
     1561 tcaaatttga agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta
     1621 atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg
     1681 tatatataaa caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat
     1741 ttcgggaaga caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag
     1801 ctatctctca gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga
     1861 aattacttgg tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg
     1921 atttcctcaa agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca
     1981 agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg
     2041 cttatattgc cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc
     2101 agcactatca agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc
     2161 aagctttgaa caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa
     2221 ataaaagtgc attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg
     2281 ctgatgtcaa aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt
     2341 acagtttttc atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt
     2401 ataaaggaac attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg
     2461 tttttccaga aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag
     2521 ggaatattat agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag
     2581 gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg
     2641 cttcctcctt cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac
     2701 aacccgatta ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg
     2761 gagctaccat tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc
     2821 tgattggtta cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca
     2881 gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga
     2941 caacaggtgg gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg
     3001 aacatgaagt tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa
     3061 cagcttttga cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg
     3121 gtgatcttct tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga
     3181 tctcacaaga agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat
     3241 ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg
     3301 acaacggcac tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag
     3361 acagttcttg gggatcttat caagcaacgc gtgtcagtag tgatgtatca caagtagact
     3421 taaaaactgc tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg
     3481 gtgagaaaaa atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc
     3541 caattagtat gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc
     3601 tagctgatac tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa
     3661 tgtattctgt ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag
     3721 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
     3781 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
     3841 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
     3901 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
     3961 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
     4021 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
     4081 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
     4141 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
     4201 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
     4261 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
     4321 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
     4381 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
     4441 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
     4501 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
     4561 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
     4621 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
     4681 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
     4741 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
     4801 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
     4861 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
     4921 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
     4981 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
     5041 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
     5101 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
     5161 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
     5221 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
     5281 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
     5341 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
     5401 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
     5461 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
     5521 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
     5581 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
     5641 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
     5701 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
     5761 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
     5821 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
     5881 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
     5941 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
     6001 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
     6061 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
     6121 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
     6181 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
     6241 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
     6301 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
     6361 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
     6421 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
     6481 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc
     6541 ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca
     6601 tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt
     6661 gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc
     6721 catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat
     6781 ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct
     6841 ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt
     6901 tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga
     6961 tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag
     7021 ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc
     7081 ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt
     7141 tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg
     7201 ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg
     7261 aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt
     7321 gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg
     7381 tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg
     7441 ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag
     7501 gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt
     7561 cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca
     7621 tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa
     7681 tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac
     7741 aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac
     7801 tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag
     7861 aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg
     7921 gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag
     7981 acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca
     8041 aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt
     8101 tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt
     8161 tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta
     8221 catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg
     8281 cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag
     8341 ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac
     8401 cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag
     8461 taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt
     8521 attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga
     8581 agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa aattctcctt
     8641 gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac
     8701 ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg
     8761 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
     8821 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
     8881 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
     8941 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
     9001 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
     9061 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
     9121 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
     9181 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
     9241 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
     9301 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
     9361 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
     9421 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
     9481 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
     9541 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
     9601 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
     9661 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
     9721 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
     9781 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
     9841 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
     9901 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
     9961 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
    10021 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
    10081 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
    10141 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
    10201 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
    10261 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
    10321 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
    10381 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
    10441 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
    10501 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
    10561 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
    10621 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
    10681 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
    10741 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
    10801 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
    10861 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
    10921 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
    10981 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
    11041 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
    11101 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
    11161 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
    11221 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
    11281 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
    11341 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
    11401 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
    11461 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
    11521 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
    11581 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
    11641 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
    11701 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
    11761 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
    11821 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
    11881 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
    11941 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
    12001 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
    12061 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
    12121 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
    12181 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
    12241 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
    12301 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
    12361 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
    12421 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
    12481 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
    12541 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
    12601 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
    12661 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
    12721 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
    12781 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
    12841 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
    12901 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
    12961 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
    13021 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
    13081 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
    13141 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
    13201 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
    13261 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
    13321 caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat
    13381 tctctttaca tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt
    13441 caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat
    13501 cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt
    13561 gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca
    13621 aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac
    13681 tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag
    13741 tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt
    13801 aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca
    13861 gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat
    13921 ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt
    13981 cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag ataaacgttt
    14041 taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga
    14101 aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc
    14161 tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc
    14221 aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt
    14281 ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg
    14341 aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg
    14401 acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac ctatactaca
    14461 atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg acgagaacac
    14521 tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt
    14581 gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcattt
    14641 aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact caggaggaga
    14701 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
    14761 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
    14821 aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag aaaaaccaga
    14881 tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa caaatccaga
    14941 cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag aaaaaccaga
    15001 tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa aaataaacaa
    15061 aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa
    15121 aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc aatcagctga
    15181 aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa taaacagcta
    15241 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
    15301 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
    15361 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac cccaaaatac
    15421 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
    15481 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
    15541 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
    15601 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
    15661 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
    15721 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
    15781 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
    15841 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag agcgacaaca
    15901 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
    15961 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
    16021 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
    16081 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
    16141 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
    16201 tgatgctcgt aaaaattaat aacggactgg atcatgtcaa atgtttgatg aataagcatc
    16261 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
    16321 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
    16381 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
    16441 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
    16501 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
    16561 tgatggaata tgaaaataag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
    16621 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
    16681 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
    16741 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
    16801 ccttgagcag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
    16861 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
    16921 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
    16981 agatactggg tataggtaag attcagcggc ttgaggaggg gagtgtagtg tctaatgatt
    17041 tctttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
    17101 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
    17161 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
    17221 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
    17281 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
    17341 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
    17401 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
    17461 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatgc acttctccag
    17521 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
    17581 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
    17641 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
    17701 atatacaaaa aatatcagag acaaaaaata atctcatcat aaacattaca tatatttctc
    17761 aaaaagttgt tttctttctt caaaaattac ttcacgatgg acctgatgaa gcatccataa
    17821 ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat
    17881 ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga
    17941 gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag
    18001 agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa tgacttcatt
    18061 attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata
    18121 cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc ctttatgcat
    18181 aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat accagctaaa
    18241 gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt
    18301 aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat
    18361 ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac
    18421 ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat
    18481 ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga
    18541 tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga
    18601 taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag aaacaacgaa
    18661 tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag
    18721 gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa
    18781 agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc
    18841 tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt taaaaaaggg
    18901 ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca ctaaaataac
    18961 tatagctcgg tcaaagccta aagcagcttc cagtaatatt cctagaccgc tgacacctcc
    19021 agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata tgaagagggc
    19081 aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt attcacttcc
    19141 ttaattcatt aataatactc ttatcttatt ataacttact tttcacctta agttaacttt
    19201 aattaataaa aaaattaggc tagtctaaat tattatgttt cattcttcta gaatatactt
    19261 ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa
    19321 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
    19381 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
    19441 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
    19501 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
    19561 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
    19621 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
    19681 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
    19741 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
    19801 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
    19861 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
    19921 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
    19981 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
    20041 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
    20101 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
    20161 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
    20221 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
    20281 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
    20341 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
    20401 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
    20461 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
    20521 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
    20581 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
    20641 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
    20701 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
    20761 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
    20821 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
    20881 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
    20941 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
    21001 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
    21061 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
    21121 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
    21181 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
    21241 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
    21301 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
    21361 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
    21421 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
    21481 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
    21541 tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta
    21601 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
    21661 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
    21721 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
    21781 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
    21841 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
    21901 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
    21961 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
    22021 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
    22081 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
    22141 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
    22201 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
    22261 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
    22321 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
    22381 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
    22441 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
    22501 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
    22561 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
    22621 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
    22681 acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc
    22741 attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag
    22801 ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg
    22861 ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat
    22921 tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa
    22981 gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg caattcatca
    23041 ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga
    23101 agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag
    23161 gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga
    23221 tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata
    23281 ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt
    23341 taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat
    23401 gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat
    23461 ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt
    23521 ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc
    23581 aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa
    23641 cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct
    23701 atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag
    23761 caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct
    23821 tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca
    23881 tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct ggttctactg
    23941 atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg
    24001 cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta
    24061 aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg
    24121 attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat
    24181 tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc
    24241 ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga
    24301 aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt
    24361 acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg
    24421 caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac
    24481 tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat
    24541 gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac
    24601 tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt
    24661 tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt
    24721 tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca
    24781 gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct
    24841 ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca
    24901 aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat
    24961 gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg
    25021 acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat
    25081 ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt
    25141 tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt
    25201 atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt
    25261 aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa
    25321 ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta
    25381 tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga
    25441 aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg
    25501 tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat
    25561 gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct
    25621 gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt
    25681 tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt
    25741 caaaacaaga ttcaaagata agcgtgtcga atgaca
//
LOCUS       DACEND010000029        25532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEND010000029 DACEND010000000
VERSION     DACEND010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.030"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW746_001002"
     CDS             complement(159..1469)
                     /locus_tag="IW746_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1077917.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW746_001003"
     CDS             complement(1481..2899)
                     /locus_tag="IW746_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1077918.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW746_001004"
     CDS             complement(3139..4707)
                     /locus_tag="IW746_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1077919.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW746_001005"
     CDS             complement(4711..5121)
                     /locus_tag="IW746_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1077920.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW746_001006"
     CDS             complement(5118..6041)
                     /locus_tag="IW746_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1077921.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW746_001007"
     CDS             complement(6038..6916)
                     /locus_tag="IW746_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1077922.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW746_001008"
     CDS             complement(6903..8240)
                     /locus_tag="IW746_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1077923.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW746_001009"
     CDS             complement(8243..9346)
                     /locus_tag="IW746_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1077924.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW746_001010"
     CDS             complement(9420..10928)
                     /locus_tag="IW746_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1077925.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW746_001011"
     CDS             complement(10894..11586)
                     /locus_tag="IW746_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1077926.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW746_001012"
     CDS             complement(11608..12582)
                     /locus_tag="IW746_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1077927.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW746_001013"
     CDS             12882..13754
                     /locus_tag="IW746_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1077928.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IW746_001014"
     CDS             complement(14212..16410)
                     /locus_tag="IW746_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1077929.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IW746_001015"
     CDS             16642..17634
                     /locus_tag="IW746_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1077930.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IW746_001016"
     CDS             17694..19115
                     /locus_tag="IW746_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1077931.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IW746_001017"
     CDS             19108..20607
                     /locus_tag="IW746_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1077932.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IW746_001018"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IW746_001018"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1077933.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IW746_001019"
     CDS             22199..22882
                     /locus_tag="IW746_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1077934.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IW746_001020"
     CDS             22884..23720
                     /locus_tag="IW746_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1077935.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IW746_001021"
     CDS             23731..25485
                     /locus_tag="IW746_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077936.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7787 a         5196 c         4480 g         8069 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ct
//
LOCUS       DACEND010000030        23434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEND010000030 DACEND010000000
VERSION     DACEND010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.031"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            222..1223
                     /gene="ccpA"
                     /locus_tag="IW746_001022"
     CDS             222..1223
                     /gene="ccpA"
                     /locus_tag="IW746_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1077937.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1560..3905)
                     /locus_tag="IW746_001023"
     CDS             complement(1560..3905)
                     /locus_tag="IW746_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1077938.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4184..5086
                     /locus_tag="IW746_001024"
     CDS             4184..5086
                     /locus_tag="IW746_001024"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1077939.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5299..7074
                     /locus_tag="IW746_001025"
     CDS             5299..7074
                     /locus_tag="IW746_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1077940.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(7140..8726)
                     /locus_tag="IW746_001026"
     CDS             complement(7140..8726)
                     /locus_tag="IW746_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1077941.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            8977..9201
                     /locus_tag="IW746_001027"
     CDS             8977..9201
                     /locus_tag="IW746_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1077942.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9342..11093
                     /locus_tag="IW746_001028"
     CDS             9342..11093
                     /locus_tag="IW746_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1077943.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            11093..12877
                     /locus_tag="IW746_001029"
     CDS             11093..12877
                     /locus_tag="IW746_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077944.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13203..14054
                     /locus_tag="IW746_001030"
     CDS             13203..14054
                     /locus_tag="IW746_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1077945.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            14151..16031
                     /locus_tag="IW746_001031"
     CDS             14151..16031
                     /locus_tag="IW746_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1077946.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(16071..16973)
                     /locus_tag="IW746_001032"
     CDS             complement(16071..16973)
                     /locus_tag="IW746_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1077947.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            17204..18607
                     /locus_tag="IW746_001033"
     CDS             17204..18607
                     /locus_tag="IW746_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1077948.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18607..20040
                     /gene="ascB"
                     /locus_tag="IW746_001034"
     CDS             18607..20040
                     /gene="ascB"
                     /locus_tag="IW746_001034"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1077949.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            20185..21111
                     /gene="trxB"
                     /locus_tag="IW746_001035"
     CDS             20185..21111
                     /gene="trxB"
                     /locus_tag="IW746_001035"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1077950.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21325..23052
                     /locus_tag="IW746_001036"
     CDS             21325..23052
                     /locus_tag="IW746_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1077951.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            23124..23417
                     /locus_tag="IW746_001037"
     CDS             23124..23417
                     /locus_tag="IW746_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1077952.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
BASE COUNT         7426 a         4170 c         5034 g         6804 t
ORIGIN      
        1 tacgttattc atttggcaac ctactttcta aattaatttt gtattcaata attgtaccac
       61 tgatttatta aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca
      121 gagataaaac ggttgcaatt cttgagatta tttgatattc tattttagaa atgtaagacg
      181 tattttcaga aaacatataa aatatgaatg ggagaacaca aatggaaaag caaacaatca
      241 ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa
      301 atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtc atcgatcgct
      361 tggattatcg tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca acaacagttg
      421 gggtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt ggtatcgatg
      481 atgtggcaac aatgtacaaa tacaatatta tcttagcaaa ttcagatggc aacgaccaaa
      541 aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggacggcgtg atcttcatgg
      601 gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact cctgttgtat
      661 tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat tatgtatccg
      721 caacaaaaga tgcagtaaat aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca
      781 gtggtgcgtt gatcgatcct atcaatggac aaaatcgtct gaaaggctat aaagaagcat
      841 taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa tacaacttca
      901 aagatggtct atctctagcc gatcggatcc ataatagcgg tgcaacagct gtctatgttt
      961 cagatgatga attagccatc ggtattttgg atggtttatt agatcgtggc gtgaaagtac
     1021 cggaaggatt cgaaatcatc acaagtaaca attcactact gacagaagta gctcgtcctc
     1081 gtttaacaag tatcacacaa ccattatacg atttaggggc ggtatctatg cgcctattga
     1141 cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg
     1201 aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg agcctgccgt
     1261 ttaactctgc gttacaagga gcaatttcaa aaaacagcat ttcatgtttg atgaatattg
     1321 tgactcgtga ttcggagttg gcaggagaac accgtttatt cgggaaaagg ggttatgaca
     1381 aaattttgtt ataatccttt ttttgtgtct taaaaaagag agaaagtggt tgaaattaca
     1441 tttatcgtat ataaaacaat ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa
     1501 aataagggac tgtgacaata ctattgtcac aatctcttat tactgtgtat tctattaaat
     1561 caatcatctt tctcatctga ttctttggtt gcttgattcg ctcttgcata agctgaagcg
     1621 gtgttccagt aatctgtata gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt
     1681 ttcttcggtc catttttcgc ccatagcgat tcaactgttt ttccaggtgt ttggatggat
     1741 tttttattga cagtaacttt tccaggtgtt tgaccagtaa atccagatac ttttttcttc
     1801 atcacatcag atgataattc gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg
     1861 taagcttgat tgatcagatt ggcaagataa ttagccgaac gtatccttgc tttgctgtcc
     1921 attccagtat tgtcatcgtg ccctgcccag ctactgatgg taatagacgg agtagagaca
     1981 attagccatg aatctcggta ttcattggta gaaccagttt ttccgaccca atctgctttt
     2041 cctagatttc cgtttaagct tgagatggca tctttgaatg gtgtagtgat ttttgcattg
     2101 atcacactgc gcatcatgtc attcatgatc gatgccgttg cttccgaata gatccggaca
     2161 gagttgcttt cgtgtttata aattacattt cctgcgttat ctgtgatgga atcgatgata
     2221 tagccttctt catagacgcc gccgtttgct aaagcctgaa atccattggt ctgttgaagc
     2281 gtcgtgacat ctactgtacc taaaggagca gattcaacgc cccaattgtc atttgccggg
     2341 tagttcatct ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac
     2401 acatcttgat ataagtgata agccgggata ttgttggacc attctagtga ctcacggacg
     2461 gtttggaagg tgttcgatcc ttcgttagta gcattgacga ttttctcgcc agcattttct
     2521 ccttcttgcc aagttgttgc ataatctgat acacgggatt ctgatccgat aagccccatg
     2581 tcgattgcag gtccataaac aagaactggt ttgattgatg agccagcttg acgttctgta
     2641 tcgaaggcat ggttattttg attctgactg taattccgtc cgccaacgaa tccgtagatg
     2701 cggccagttc tgttatccat caatacattt ccggtttcta cttgactgcc agcaccatca
     2761 tctaacatgt atccataatt ttctacgcca ttttgcaatg cagcatagac atctttatcg
     2821 attgtcgaat ggattgtata tcctttattt tggattgttt gctgtgcctt ttgataataa
     2881 gcgtcactga ccgatgaatc agacatatca gcgttgtctt tttcagccaa ttgttgtgca
     2941 atgattcttg ttgcttcatt catcacagta taatacaaga attctctgtc agtctgttct
     3001 gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg cttttgcttc ttcgtattgt
     3061 tctttcgtaa tttggccttc acgatacata ctgaataaaa caaaatcttt tctggcaagg
     3121 ccggcagaaa gatcttcttt tagcgcacct gtgttcgtgt atggactata cgtgatcggg
     3181 ctttggggta atccagcgat ataagcagct tgcggcagcg tcacatcttt tgcatctaca
     3241 ccaaagattc ctcttgcagc ttcctggact ccagcaatgt tttgtccctt attattacga
     3301 ccaaatggtg agacattcaa atacgtagca atgatctcat cttttgaaaa gtatttttct
     3361 acttgtgcag aaagaatgat ctcgtttgct ttacgtttga atgttgtttc atctgtaagg
     3421 atctgctgtt tgactaattg ctgtgtcaaa gttgatccac cagaagagcc aataccagta
     3481 gcttcagata ctaacgctcg taaaacagct ttaggaacgt aaccttggtg tttatcgaag
     3541 tattcatcct ctgtgctgat aattgcagtt ttcaataaag gtgaaatttt atcagatgaa
     3601 atagtcgtgc gcatcagatc tgtctggatt tttgatatct ctgtattatc tgcataaaca
     3661 atctttgacg tttctgtaat gtttcctagt tctgtctgca gtgctttttt agtcggcaat
     3721 tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta ttccaatacc tagagctgcg
     3781 aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt gaaaaactct aaaaataatc
     3841 ttaggaacaa accatttttc tttttttctt ctattctgtt tttttcgagt atttttgcgt
     3901 ggcaaaaggg tcacctcaac taatggtttt atttcgctaa ttatagcaaa taatagaaca
     3961 aaaaaacaga caaggctgtt tttggcagga atttcttttt taaaggagtt aaaacgtgag
     4021 aaaatgatct tttttataaa agagttctta agcttttctt tagagaaaat ttcagttact
     4081 atggagaaca gggcaaagaa aaatcaatca ttttctttgt atatcatctt accaatatat
     4141 aggaattgtt agtaacaatt gcctttcaag gaggaatcca tccatgttat taggctcaca
     4201 cgtcagcatg agcggaaaga aaatgctgtt aggagcagca gaagaagcag ccagttatgg
     4261 atcttcgact tttatgatct atacaggcgc ccctcaaaac actagaagaa aagccatcga
     4321 agagatgaat attgatgccg gaaaagcgta catggcagaa aacggtctaa caaatatcgt
     4381 cgttcatgcc ccttatatca ttaatttggg gaatacgatc aaaccagaaa tgtttccttt
     4441 tgccgtccaa tttctgagag aagagattgt tcgtgcggaa gctttaggcg ctactcaaat
     4501 tacacttcac ccaggtgctc atgtaggggc aggaacagaa gccggaattg ctagaattat
     4561 tgaaggcttg aatgaagttt tgacaaaaga acagacggca caaatcgctt tggagacaat
     4621 ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg
     4681 agtaaaattg aatgaaaaat tatccgttac ttttgacacc tgtcatacga atgatgccgg
     4741 ttataatatt aaagaagact tttcaggtgt aatggaagag tttgataaga tcatcgggtt
     4801 agatcggctt aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga
     4861 ccgacacgcc aatattggtt ttggaacaat cggattcgac acgctgaata agatcgtcca
     4921 tgatccaatg tttgacaata tttcgaaaat cttggaaact ccctatgtcg gcaaagataa
     4981 aaagaaagct tacgcaccat atggaaaaga aatcgccatg ttcaaagcac aaatattcga
     5041 tccagaagtg tttcctgagt tattagcaga agaagtgata ctctaagatt ttctatctga
     5101 cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag
     5161 tgctttttgc agtctttttc atcatgcatc ttctcccccc tctttttaaa tttagacaaa
     5221 cagggtatac tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt
     5281 gattggaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca actaaaaaag
     5341 aaacatacat actttattct tggcatagcc ctattattta caattattcg tttgtattta
     5401 tatagtaaag caatctatgg aacagacatg tcggcaacct atgatgacca gctgctgatt
     5461 cgctatgctc aaaatatttt ggacggtaaa tggctagggg agtatacgac tacaacactt
     5521 tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca tctttcttat
     5581 gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact cgcgtttaaa
     5641 ccgatcgtga agaacaagta tcttctttta agtttctatt tttatttttt gttttctccg
     5701 attagtttta cacatgaata ttccactcga acgtatcgta atgccctcgt gattccggct
     5761 gttgagatca ttatcgcttg tttactagct atttattacc gcaaatacgg ttctagcaga
     5821 aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg gttcattcgc
     5881 gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag tttcgtccag
     5941 atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt
     6001 cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat agttggcttt
     6061 tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat tgcaaccatc
     6121 aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg aatagatgat
     6181 gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc actaaataaa
     6241 gcattagccg catctcctac attccatact gtttctaccc gtattgacga attatatacg
     6301 actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat catcttttgg
     6361 gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa agaaaccaat
     6421 gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg aaccatcaaa
     6481 aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag tgatctacca
     6541 atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa agatttccat
     6601 caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca agaattatta
     6661 aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc taatttccaa
     6721 ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt tctggctatt
     6781 cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac cgtatttgca
     6841 aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt aatactgaca
     6901 caattggttt ttcttttcgg tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat
     6961 tattttttaa gtgtctacac tggcgcaggc gtgccgttaa cgcaattcat cgtagtaaca
     7021 agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa ataaaaagac
     7081 ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa aatcagtttt
     7141 tattcgattt cttctaccat ttcctgcaaa cgctgttcat attttttgtt tcctttatat
     7201 aaatcaccaa tcgtccatgc caacaggagg aggacaagaa caatgattac ataagcgatg
     7261 tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt
     7321 cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca gccaagaagt
     7381 tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt ggtcatcatc
     7441 agtaaaggga tcatcgaaaa cggcaacgca aaagccaaga acacctggga ttcattcatt
     7501 aagttattca aagcggtatg ttcttcgatc gttccttttg tacttgtaaa caaaacacaa
     7561 atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca aatcgggata
     7621 cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt aatagtagag
     7681 ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc agattttgct
     7741 acagcaatca agataccatt gctcatagct gatggatcag ataaagcttc atacagacca
     7801 aaaaaggaag gatcttgaac agaaccggtt ttgaagacag cgacacccat aatcaatagc
     7861 agcgcattga ctagaaaagc catcgacaat tggatattag agtcccaagt agaaaaacgt
     7921 acagcttgtg ctacatcttc ttcgtcttgg tgatcgatct tccgagtttg tgaaactgct
     7981 gaatgtagat ataagttatg tggcatgact gttgcgccaa taatccctaa tgctccagtt
     8041 agcggagttt cgccaccaat gacaggtgag tcggagaatg tatggctgtt agggataaag
     8101 ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac aaaaacgaat
     8161 aaaatgacga aaatcaagca gacaaccagt gcttcgatct tacgaaaccc gacttttgtc
     8221 agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac caatggaata
     8281 tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcaatatc tgttgccata
     8341 atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt gcgtgctcgg
     8401 attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat atactgtaaa
     8461 agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg gaaattttgt
     8521 cctccggtga tcgaagtaga ccagttaccc gggtccatat acccaactgc tactaacgca
     8581 ccaggtccag agtaagcaag aagtgtttta aaaaaacttt tcccctttgg tacatccact
     8641 gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg atgcttatga
     8701 aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat ctacgattac
     8761 attctacacc tcaaaaagaa aaaattaaag aaaaagattc ggtatgccta attttttttc
     8821 gtggcgtgtc ttgctagatg acgaaagaaa ttttgttctc ctctaaaaaa accactattt
     8881 tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta aaataagtca
     8941 ttgagagcaa ttcggaaata gaaaggagag atttatatgt tatcaacagg tattgtcgta
     9001 cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa
     9061 tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg
     9121 atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat gcagcaaatg
     9181 aagaaccaag ggaaaaaata ggttctgacc caacgacttt ttacgggctg attttttcag
     9241 tccgtttttt atttcgtaat ttgttagact attcagatta ttcagcaaaa agacaagcac
     9301 agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt tttagaaagt
     9361 taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc tcgctattcg
     9421 cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt gatgagattg
     9481 ccgataaaaa tttatcagta aagatcattt tcgggtggat cttcctattg attatcgctg
     9541 cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga agtgcagctc
     9601 ggttggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg gacaatctct
     9661 tttaccaaaa atatcggact ggggatttga tggctcatgc aacgaatgat ttgaatgcga
     9721 tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt acgggcggaa
     9781 caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt gcgctgattc
     9841 ctctgccttt gctggccgtc acttcacgag tgctggggtc gaaactccat gatgcttttc
     9901 gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt atcactggga
     9961 tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt gaagaaaaga
    10021 ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta tttgatccat
    10081 tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg
    10141 tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat atcggtatgc
    10201 ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga ggaaatgctt
    10261 cttatgatcg cgtcaatgaa cttttgcatg aaaaaacgca tatcgtggaa caactgggtg
    10321 cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt aattatccga
    10381 aagaagaatc accagcatta gaacaaatca agtttgcaat ccaagaagga gaaacattag
    10441 gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg atgcgtgaat
    10501 acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa tattcattga
    10561 atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt tcgatgacca
    10621 ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta gagcatgcgg
    10681 cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat gataccttag
    10741 ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg attgctcgag
    10801 cattgatcaa aaatcctgaa ttattaatat tagatgatgc actttctgca gtagatgcca
    10861 aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa acaactatca
    10921 tttcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag
    10981 ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc tggtatgaac
    11041 aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc tgatggatga
    11101 aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa agatcatcaa
    11161 acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta tcttatttgc
    11221 gtttgctctg tctgtgatca atgtcctatt gccaaggatc atccaaactt ttatggatga
    11281 tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg gtatgtactt
    11341 atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt attccatggc
    11401 ttcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc atacactagg
    11461 tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga cgaatgacac
    11521 agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga tctttgcagt
    11581 cgtttcttca ttcattgcca tgtttcaaat caatggagaa attgccttgt ggactttggt
    11641 gttcttgcca attttaggaa tagtcatctg gtattaccag aaatttagtt ctcgtattta
    11701 ccggaatatg cgggaaaaat taagtcagct gaatacgaaa ttgaatgaat cgatttccgg
    11761 catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat ttgaacaagt
    11821 gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acaaattcga ttctgttaag
    11881 tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt tgtttgggat
    11941 cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga cctatgtcca
    12001 gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca cagatgggat
    12061 ggttgctggt agtcgtatct tgaagattat ggatacagaa gaatatgcac cacaacaaaa
    12121 ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg taagcttttc
    12181 ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc cgggagagac
    12241 cgttgcatta gtgggacaca ccggaagtgg gaaaagctcg atcatcaatg tgttgatgcg
    12301 gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc gagaatatcc
    12361 aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct tgttttatgg
    12421 ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac agatacgcca
    12481 tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag gatatcactc
    12541 aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga tcacttttgc
    12601 gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag caaatatcga
    12661 tactgaaaca gaaggattga tccaagaagg attggcgaaa atgcgacaag gacggacaac
    12721 gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt tagttttaga
    12781 caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa atggtttgta
    12841 tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac agtagcattt
    12901 tgacctaagc actagagtga aaataaaaca tagcttccca tatttttcat ctgtgagagt
    12961 tagtgtcgta gttaccagat ttaaaaaaga cgacgtcagc tagtcagaag tgcctaatag
    13021 cccctaagaa agcgttgaca aaatcgtttg ctttcgataa tataaagaaa acgatacaac
    13081 ttggatagtg attgcctgat gcaagctaaa cctaatgagt ctcccatggt ttcatggacg
    13141 gctttttggg tttttctttt tgttttggat acagccctat atatcatgga aaggagcaaa
    13201 acatgctgat tgagaaagta ctgaataaca acgtcgtaat aacaagggat gaaagagatc
    13261 gggaaatgat tgtgatggga aaaggattgg cattcaaaaa gaaggtgggt gacccgattc
    13321 ttgattcatg catcgacaaa aaatttcagt tgacaaatga cggactttca caacagttcc
    13381 aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa atcattgaat
    13441 atacaaaaga ggctctgcaa gtccatttaa atgattctgt gtatatttca ttgacggatc
    13501 acttgtatac agctatcgaa cgtcaaaaaa tggggatctt tgtgccaaat gtattgctgt
    13561 atgatatcaa acaactgttt cctaaagagt atgccattgg gaaaaaaaca gtgaagaaaa
    13621 tctcggataa ataccacatc aagttatcag aagacgaagc cggatttatc gcattgcatt
    13681 tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt accacaacaa
    13741 tacaggatat tttaaatatc gtccgctatt actataagat tacatttgat gaagattcag
    13801 tccacttttt tcgattcgtc acacacctgc gtttttttct gacacgtgtt ctagatggag
    13861 cgaaatcaga agatggagaa gtttctgagg aattgttcca agttgttcaa aaaaaatata
    13921 aggatacgtt tggttgcgtt gagaaaatag cagacttttt aaccggggct ttcgactacc
    13981 agatgtccac ggatgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga
    14041 atcgttgtaa ataaataaaa aaggaggaca aattaacaag aacgtatgaa aataagggta
    14101 gtgacattca gttggctaaa ccttccacta attaattgga gggaatagaa atgggaaaat
    14161 accaggaatt agcagaaaac atcgtaaaga atatcggagg aaaagaaaat atccgtagct
    14221 tgacacactg tgtgacgcga cttcgtttca aactaaagga ggaaaataaa gcaaaggatg
    14281 atgttttgaa aaagatggaa gggatagtca ccatcatgaa aagcggcggg caatatcaag
    14341 ttgtaatcgg gaatcatgtg ccagatgtct atgcagaagt actgacagta gccgggattt
    14401 ccgatgaagt agaagaagag gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca
    14461 tattaagtgg ctgtttccag ccttttttag gtgctttagc tgcagcggga atggtcaaag
    14521 gattaaatgc tttgttcttg tttctcaaat tttatactga aggatcgggt acgtacatca
    14581 tgctgaatgg gataggggat gcaatttttt atttcatgcc agtgatctta gggtacacag
    14641 cagcgaaaaa atttcatctg aatccgatgg tggggctcgt gattggtgca gcactttgtt
    14701 atccgactgt acaaggagca gcactacaaa gtggtttccc tgatggcagt cctgcgcctt
    14761 atagcttgct gggtcttccg gcttatgaaa catttttggg tatcccatgg gtgggcagca
    14821 gttataccag tagcgtcgta cctgttatct tcattatcgc atttgccgga caagtccaaa
    14881 aaattgccaa gaaatggatt cctgcagtcg tccagacctt tttggttcct ttttctgtgt
    14941 tattaatcgc acttcctgct ggctttttga ttatcggtcc gattgtcagt atactgacag
    15001 atctattaag cacgggattc caagcattga tggacttttc tccagctgtg tatggtgcag
    15061 ttctagggtt tctttggcaa gtactggtga ttttcggact tcattggagt attatccctc
    15121 ttgcgattat gcagctagcg caaaatgggt ggagtcagat gttagtaggc gtttatggcg
    15181 cgagttttgc acagacagca gtggtattgg cgatgtattt caagttcaag gatcaaaaaa
    15241 tgaaggcgct ttgcatgcca gctgtgattt caggaatctt cggcgtaaca gaaccggcta
    15301 tttatggaat caccttaccg aagaagaagc catttattta ctcgatgatt ggtggtgcca
    15361 tttcaggaat ctatttgatg gcatctggaa caatcagcta cacaatgggc ggattaggga
    15421 tttttggggt ggtaaactac atcacggaaa cagacgccag tggtatgttt caagcgttta
    15481 tcggcattgc tattgcttcg gtaatcgggt tttcgctgac ttttttcttt tggaaggatg
    15541 aagcaggtga aacagatgct atacagaaaa aatcaggtaa agaaattgta aaaacaccga
    15601 tacaaggaca aatcgctcca ctaaatgcag cgaaggatgc agcttttgca caaggaacac
    15661 taggtcgagg aatcttgatc taccctgaaa aaggagaagt acgtgcccct tttgacggaa
    15721 cagtcatgac cctgttcccg acgaaacatg caattggaat ggtttctgaa acagggctgg
    15781 aattgctgat tcatgtcgga ttggatactg tgcagttgga agggaaatat tttgaaagct
    15841 tagtgcagca aggagaaaga gtgaaacaag gcgatctgtt gctacgcttc gacaaaaaga
    15901 aaatcgaaaa ggaaggatac agtttagaaa cacctgtgat cgtcaccaat tacacggatt
    15961 atttggatat tttggaaaac caaggggaga cggtcgggcc agacgatgca cttatcaccg
    16021 cactgacctg aacggttagg tgaagaatgg aataggctta actattcttt ttattccact
    16081 tctttatttt caaaattgaa aggctctttc atgatggctg agctgatagg gcgtttatcg
    16141 aatttttgac cgacagaaat tagatggact aaattctgtt gataattttc tgcaaaaaca
    16201 caactatata acacgtctag aagataacaa attgatgtat tgatcgtaaa attaccaatt
    16261 tttgaataca gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc
    16321 gttgaatcac cgatactggt caaagctaaa attggaacat gattttgttt caatatttct
    16381 gcggtacgaa gaatcacttt attttcaccc gtataagaaa tcaggatccc gcacatatcg
    16441 ctcgtgcaat tgtacgcttc atagaatgat tcaccaaaag tggtgacaat tgtgacatcc
    16501 attttgatgc gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg
    16561 gcgaagatca cgattttttt tgcctggagc agtaaatact tggctttttg cagttggtca
    16621 tgatgaatga gagaaagtgt atcttgaatc gtcatctgtt ctaaagaagc cattttattt
    16681 gcgatcgtca taataccgtc tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa
    16741 ttcccttcaa gataggctgt ttcagctaaa aaagcattac gaaaactgac ccagccatca
    16801 aaacccattt tttttgctac acggatgagt gtagacgggt ggacatagca tcgctcagcg
    16861 atctcttgga tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa ctggatcact
    16921 tcattttctg aaggagaaaa ggagatttct ttgatttttt cacgtaataa catgtagtta
    16981 ccttcttctt atttttttac gtaaattata acttttttga aaatgttttg taaacggatt
    17041 tatactatat aacttaaaat agactttttt tgacaagata tttacaaaaa tccactagaa
    17101 aacagtaact tttgtaaatg ttactggaaa gaaacggatt tctattgttt gttttgttta
    17161 caaaaaagat aatgaaagtg taaaaaagta aaggagtgag aacatggcaa aaggaatcaa
    17221 aatcgctacg attggtggag gcagcagtta cacccctgag ttgatggaag gatttatcaa
    17281 aagatatgag gaattaccga tacgagaaat ctggctcgta gatgtagaag aaggaaaaga
    17341 aaagcttgag atcgttagtc agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt
    17401 caaaatctat gcaactctgg atcgggaaga agcattgaat gatgcggatt ttgttacgac
    17461 tcagtttcgt gtcggtcaat tagatgcacg aatcaaagac gagcggatcc ctagttatta
    17521 tgggatgatt gggcaagaaa caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt
    17581 tccagtggtc ttggcaatcg ttgaagacat gaagcgttta tgtccaaatg cttggctgat
    17641 caatttcgcg aacccaagtg ggatggtgac agaagcagtt atcagatatg gcaaatggga
    17701 taaagtgatt ggattatgca atgtgccagt aatggcgatg atgacagaac cagcaatgct
    17761 tggcaagaaa aaagaagagc tgatctacaa gtttgccggt ctaaatcatt tccattggca
    17821 caaagtagca gataataaag gaaatgaccg cacggcagaa ttgattgatc gtttatacga
    17881 tgaagacaat ggattgccga aaaatatctt tgatatcccg tttttcaaag aacaattaaa
    17941 acaaatgaat atgatccctt gtggctacca ccgctattac taccgtgaag aagaaatgct
    18001 ggaacatgct ttagaagaat atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac
    18061 agaagcagag ctatttgaat tatacaaaga tccagaatta gaccataaac ctgaacaatt
    18121 gcaaaaacga ggtggcgctt attattcaga cgctgcctgt gaaacgattg cttcaatcta
    18181 tggaaataaa gaaacacaga tcgtggtatc tacaaagaat gatggagctg ttcctgacct
    18241 gccggcagat tgtgtagtag aagtcactgc ctatgttggc gcacaaggag cgagaaatgt
    18301 agcgtttggt tctcttcctg ctgctgaaag aggctggttg caagtaatga agaatatgga
    18361 attgctgacg atcgaagcag ctgtgactgg tgattatggg acagctttac aggcgtttac
    18421 gatcaatccg cttatacgat caggaaagac agcaaaacgg atcatggatg agttgtttat
    18481 tgcacaccag cagtatttac cgcaattcaa ggaaacaatc agccgattaa aagaagaagg
    18541 aatcgaagtt caagatgaac tggcaagaga attatcaggt aatacacata taatggagga
    18601 agtataatgg gattcagaaa agatttttta tggggcggtg ctacagcagc caatcaatgt
    18661 gagggcggtt atgatgaagg tggacgcgga ctagcaaacg tagatgttgt gccaatcgga
    18721 aaagaccgtt ttcctgttat caccggaaag aaaaaaatgt ttgaatttga tgaggatcat
    18781 ttttatccag caaaaattgc aatcgatatg tatcatcgtt ataaagaaga tattgctttg
    18841 tttgcagaaa tggggttcaa gacttatcga atgtccatcg cctggagtcg tatttttcca
    18901 atgggggatg aaaaagagcc gaatgaagct ggcttgaaat tttatgagga tgtctttaaa
    18961 gaatgccaaa agcacggaat cgagccactt gtgacgatta cgcattttga ttgtccaatg
    19021 catttgatca aagaatacgg tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat
    19081 ctgtgtcgag tcatcttcaa tcgatataaa ggacttgtca aatactggct gacattcaat
    19141 gagatcaata tgatccttca tgcaccattc atgggagcag gactgtactt tgaagaagga
    19201 gaaaacgaag aacaagtcaa atatcaagcg gcacatcatg agttggttgc aagtgccatc
    19261 gcaacgaaga ttgctcatga agtagatccg gagaacaaag taggctgtat gctggcagca
    19321 ggagcatatt atccatacac ttgcaatcct gaagatgttt gggcttctag aaaatcagat
    19381 cgagaaaatt atttcttcat tgatgttcag tcacgaggag aatacccagc ttatgcattg
    19441 aaagaaatgg aacgaaatgg cattcagatc gaaatggaac caggggatgc cgcattgctg
    19501 aaagaacata ctgttgattt tatctctttt tcttattatt cttcccgagt ttcgactacc
    19561 gacaacgaac tcttggaaca gactgcagga aatatctttg cttctgtcaa aaatccatat
    19621 ctgcaagcaa gtgaatgggg gtggcaaatt gatccattag gcttgagaat aacgatgaat
    19681 gatatttatg atcgttatca aaaaccatta tttattgtag aaaacggctt aggtgcagtc
    19741 gatacgccag atgagaatgg ttatgtggaa gatgattacc gaatcgatta cctggcagcg
    19801 catatccagg cgatgaagga tgcagtagaa ctagacggag tcgatctatt aggctatacg
    19861 acttggggat gtattgattt agtatcagct ggtaccggtg agatgaagaa aagatacgga
    19921 ttcatttatg tcgatcgtga taaccaagga aatggcacat tagcccgttc aaagaaaaaa
    19981 tcattcaact ggtataaaaa agtaattgat tcaaatggag aaaatctttc gaacgattga
    20041 tcaatgattt ataaaagtct attgtttttc agcggatttt tcgagtataa taaagctatt
    20101 cgagaaacgc aagcagaaca atagactttt tttcgcattg gctgtaaagt atgcttactt
    20161 gattaagtaa aggagaatct acatatgtat gatgtaatcg ttattggcgc aggtcctgct
    20221 gggatgactg ctgctttata tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa
    20281 ggtgcccctg gcggccaaat gaacaatacg gcagaaatcg aaaattatcc tggattcgat
    20341 tcgatcatgg gccccgatct agcttataag atgtatgaaa gtgtctcaag atttgggaca
    20401 gaaaatgctt atggaatcgt tcaagatatc aaggatcacg gcagccataa agaagtcatc
    20461 tgtgaagata aaagttacga aggaaaaaca gtcattatcg caacaggctg tatccaccgt
    20521 aaattaggcg tacctggcga ggaagaattt gctggacgag gcgtttctta ctgtgcagtc
    20581 tgtgatggtg cgtttttccg taacaaacgt ctagtagtca tcgggggtgg ggactcagca
    20641 gtagaagaag ccatctattt gacacaattt gcttctgaag tagtgatcgt ccaccgtaga
    20701 gatgaattac gtgcacaaaa gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc
    20761 tttatgtggg atacagtcgt tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg
    20821 aaagcacgta atgtcaaaac cggcaatgaa tttgatttag cagcagacgg agtattcatc
    20881 tatgttggac tggatccatt aactgcacct tttagagaag caggtttgac gaacgaagct
    20941 ggctggattc caacagacaa cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga
    21001 gatgttcgtg aaaaagattt gcgacaaatc actacagctg ttggtgacgg cggtattgct
    21061 ggacaacaag tgtataaata cttggaatct ttagaagaaa caactgcata agtttaaaaa
    21121 gagtagaaga acgggttcaa ttgttctttt actctttttt ttcgcgtgaa atttactgaa
    21181 tctgatttat gcgaacacgt ccaacacctt ttgataaaag aatcctttct tgatttaagc
    21241 gttttttggt cctcatactt tcatatctaa taagtgaatg ctatacttat taagaaccat
    21301 atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat
    21361 gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa
    21421 aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggcat gcggggaatc
    21481 ttaggacctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggacta
    21541 gcacgtttca tggatacaca aggtgaagaa gcaaaacgcc gaggtgtcgc tattgcttat
    21601 gactcacgcc atcagtcacc agaatttgca atggaagcag ccaaaacact ggcacatcac
    21661 aatatcccgt cttatgtatt cgaaagtctg cgtccaacac cagagctttc ttttgcagtt
    21721 cgtcatttga agacgtttgc gggaatcatg gtaactgcat ctcacaaccc agcggcttac
    21781 aatggatata aagtctacgg agaagatggc ggacaaatgc ctcctgcaga cgcagatgca
    21841 ttgacgaaat atgtccgtga agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa
    21901 gaagctaaac atagcgggtt gatcacgatc atcggagaag aagtggacgc tgcttatcta
    21961 gaagaaatca aagcagtgac gattgatcgt gaattggtag aaacaatggg gaaagatttg
    22021 aaacttgtgt acacgccact tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa
    22081 caagctggat tcgaacaatt cgtactagtt cccgaacaag ctgtggcaga tccagatttc
    22141 actacggtca aatcgccaaa tccagaagaa cattcagctt ttgaatatgc tatacgactt
    22201 ggagaaaaag aaggagctga cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc
    22261 gcagcggttc gtttgccaga tggttcttat gaagtattga ctgggaacca aatcggggca
    22321 ttgtttgctc aatatatttt ggaagcacat caatcaagag gcactttacc agaaaacgca
    22381 gcggtattga aatcaatcgt ttccagcgaa ttgcctacag caattgcgga acactacggt
    22441 gctaccatgt tcaatgtgtt gacagggttt aaattcattg ctgaaaaaat ccaacaattc
    22501 gaagaagacc attcacatac ttttatgttt ggattcgaag aaagttatgg ttatttagtt
    22561 aaaccttttg ttcgcgataa agacgcgatc caagcattgc ttttattagc agaagtagca
    22621 gcttactata aaaaacaagg caaaacgttg tatgacggct tgcaaacgat ttttgaaacc
    22681 tatggctatt ttgcagaaaa aacgatttct gtgacaatga gcggacaaga aggttctgcg
    22741 aaaatctctg ctttgatgga aaaattccgt aaagaagcac cgacagaatt tgccggagca
    22801 aaagttattc aaacagaaga ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta
    22861 gaaaaactaa caacaccgcc atccgacgta ttgaaatata cattggaaga aaatggctgg
    22921 attgctatcc gcccttcagg aactgagccg aaaatcaagt tttatattgg tgtaaaagga
    22981 agctctacag aagacgcagc cgaaaaagta gcctcttatg aagcgattat caaaaagatg
    23041 acagaagaat aaaaaacttg agactgatgg aaaaagtggt aaactttaga agtcagtctt
    23101 ttttcaagtt ggaggtgaat ggattgccag acacgcaaaa atcaaaagta gagaaagtca
    23161 gtcaattatt caaaatgttg agtgatccca ctcgtcttaa aattctgcta tatctgaaag
    23221 acggggaaca aaatgtgaca gcaatcaccc aagcagtgga aatggagcag tctgctgtat
    23281 cgcatcaact acgtttatta agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag
    23341 cgattttgta tagtcttgat gattctcatg tactggatat tttgaaccag acactcaaac
    23401 atgtggaaca tcaataacga taggtgcaaa taga
//
LOCUS       DACEND010000031        23291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEND010000031 DACEND010000000
VERSION     DACEND010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.032"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(110..181)
                     /locus_tag="IW746_001038"
     tRNA            complement(110..181)
                     /locus_tag="IW746_001038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Arg,seq:ccg)
     gene            241..1575
                     /gene="rpoN"
                     /locus_tag="IW746_001039"
     CDS             241..1575
                     /gene="rpoN"
                     /locus_tag="IW746_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1077953.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1778..2815
                     /locus_tag="IW746_001040"
     CDS             1778..2815
                     /locus_tag="IW746_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1077954.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            2856..3857
                     /gene="gap"
                     /locus_tag="IW746_001041"
     CDS             2856..3857
                     /gene="gap"
                     /locus_tag="IW746_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1077955.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            3967..5157
                     /locus_tag="IW746_001042"
     CDS             3967..5157
                     /locus_tag="IW746_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1077956.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5261..6016
                     /locus_tag="IW746_001043"
     CDS             5261..6016
                     /locus_tag="IW746_001043"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1077957.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            6132..7430
                     /gene="eno"
                     /locus_tag="IW746_001044"
     CDS             6132..7430
                     /gene="eno"
                     /locus_tag="IW746_001044"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1077958.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            7829..8968
                     /locus_tag="IW746_001045"
     CDS             7829..8968
                     /locus_tag="IW746_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1077959.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(9035..9610)
                     /locus_tag="IW746_001046"
     CDS             complement(9035..9610)
                     /locus_tag="IW746_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1077960.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(9615..10589)
                     /locus_tag="IW746_001047"
     CDS             complement(9615..10589)
                     /locus_tag="IW746_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1077961.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEPICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(10613..10657)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10872..11840
                     /locus_tag="IW746_001048"
     CDS             10872..11840
                     /locus_tag="IW746_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1077962.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            11937..12230
                     /locus_tag="IW746_001049"
     CDS             11937..12230
                     /locus_tag="IW746_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1077963.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            12348..12584
                     /gene="secG"
                     /locus_tag="IW746_001050"
     CDS             12348..12584
                     /gene="secG"
                     /locus_tag="IW746_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1077964.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            12696..13442
                     /locus_tag="IW746_001051"
     CDS             12696..13442
                     /locus_tag="IW746_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1077965.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            13445..15802
                     /gene="rnr"
                     /locus_tag="IW746_001052"
     CDS             13445..15802
                     /gene="rnr"
                     /locus_tag="IW746_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1077966.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            15824..16288
                     /gene="smpB"
                     /locus_tag="IW746_001053"
     CDS             15824..16288
                     /gene="smpB"
                     /locus_tag="IW746_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1077967.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            16590..17309
                     /gene="atpB"
                     /locus_tag="IW746_001054"
     CDS             16590..17309
                     /gene="atpB"
                     /locus_tag="IW746_001054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1077968.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            17362..17577
                     /gene="atpE"
                     /locus_tag="IW746_001055"
     CDS             17362..17577
                     /gene="atpE"
                     /locus_tag="IW746_001055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1077969.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            17668..18192
                     /gene="atpF"
                     /locus_tag="IW746_001056"
     CDS             17668..18192
                     /gene="atpF"
                     /locus_tag="IW746_001056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1077970.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            18179..18721
                     /locus_tag="IW746_001057"
     CDS             18179..18721
                     /locus_tag="IW746_001057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1077971.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            18744..20300
                     /locus_tag="IW746_001058"
     CDS             18744..20300
                     /locus_tag="IW746_001058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1077972.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            20314..21216
                     /locus_tag="IW746_001059"
     CDS             20314..21216
                     /locus_tag="IW746_001059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1077973.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            21239..22645
                     /gene="atpD"
                     /locus_tag="IW746_001060"
     CDS             21239..22645
                     /gene="atpD"
                     /locus_tag="IW746_001060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1077974.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            22659..23081
                     /locus_tag="IW746_001061"
     CDS             22659..23081
                     /locus_tag="IW746_001061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1077975.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         7846 a         4224 c         4975 g         6246 t
ORIGIN      
        1 atctatacag atacaacaaa aaactgtcca ccaattctct ttggcggaca gttttcactt
       61 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
      121 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
      181 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
      241 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
      301 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
      361 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
      421 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
      481 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
      541 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
      601 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
      661 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
      721 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
      781 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
      841 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
      901 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
      961 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
     1021 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
     1081 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct ccaacgtggg
     1141 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
     1201 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
     1261 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagattt cggtgtattt
     1321 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
     1381 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
     1441 aagccgttgt cggaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
     1501 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
     1561 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
     1621 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
     1681 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
     1741 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
     1801 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
     1861 tatctattgg atgcagccga ttgggcgtcg aagtttatcg gaaagtatgg ggatcacaga
     1921 acgagtctta cgaacagaaa cagacctttt gaaaaatctg aatttgatcg acacctcaaa
     1981 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
     2041 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
     2101 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
     2161 cgaagcgctg agcgaagcct tagaccgact acttccagaa ggcgagaata tcatcgctgt
     2221 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
     2281 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
     2341 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgcg cactttatgt
     2401 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
     2461 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
     2521 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
     2581 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
     2641 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
     2701 cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
     2761 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
     2821 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
     2881 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
     2941 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
     3001 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
     3061 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
     3121 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
     3181 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
     3241 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
     3301 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
     3361 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
     3421 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
     3481 actcaacagg tgctgctaaa gctatcggtt tagtaattcc cgaattgaac ggtaaattag
     3541 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
     3601 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
     3661 cttacggata caacacagac gaaatcgttt cctcagatat cgttggtatg acttacggtt
     3721 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
     3781 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
     3841 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
     3901 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
     3961 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
     4021 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
     4081 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
     4141 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
     4201 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
     4261 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
     4321 cgttttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
     4381 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
     4441 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
     4501 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgcccaat gattgcaatc
     4561 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
     4621 gataaaatct taatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
     4681 atcggtaact ctctagtaga agctgataaa gtagaactag caaaagaatt aatcgaaaaa
     4741 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
     4801 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
     4861 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
     4921 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
     4981 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca
     5041 gcagaacaat taggattcgc tgacaaattc acacacatct caacaggtgg tggcgcaagc
     5101 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
     5161 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
     5221 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
     5281 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
     5341 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
     5401 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
     5461 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
     5521 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
     5581 agacatcaat aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg
     5641 cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
     5701 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
     5761 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
     5821 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
     5881 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
     5941 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcattct tgtcattgtt
     6001 agacgctgta aaataattgt tgaaaatttg tgaaaaatgc aagaaatgtt tgcactaatg
     6061 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
     6121 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
     6181 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
     6241 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
     6301 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
     6361 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
     6421 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
     6481 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
     6541 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
     6601 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
     6661 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
     6721 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
     6781 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
     6841 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
     6901 tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
     6961 aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa aacgactggg
     7021 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
     7081 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
     7141 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
     7201 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
     7261 caatctctga tatcgctgtt gcaacaaacg ctggccaaat caaaactggt tctctatcac
     7321 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
     7381 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
     7441 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
     7501 gaactctcta cttgctgaaa tatcagcagg tagagagttt ttttgcaact agcttctgta
     7561 tatgatgttt acaaagaatg gtacaagaaa agatcatcct tgaaaaagga aagctaacaa
     7621 taactagcat catataagag cttttaagga tagaacagta gaataggaat ctatgagcaa
     7681 gtcctctcgt ataaataaaa taggagggaa gtagcagcat atttttcata aagtggctct
     7741 ctttcttcat ttttactttg gttgctccta tttgaaaatt cgagcacact caaatacaat
     7801 aaaaagaaat ggcaagggag aggattagat gaaaaaagaa gaaaaaatcg cgattttaca
     7861 agagatcatc cgaattaaat cagtgaatgg aaacgaaggt gaagtagcag cctatttgaa
     7921 taagcttttg gcaaaacatg acataacagg agagattgtt tcttacagag atggtagaga
     7981 taatttgatt gctcgttacc aaaagggcca atcaggaaaa gtgttaggcc tatctgggca
     8041 tatggatgtc gtggcggctg gtgatgaatc gtcttggacc tatgcaccct ttgcagcaga
     8101 aatccatggg aaccgtttgt acggacgtgg tgctacagat atgaagagtg gcttggcagc
     8161 aatggtgatt gccatgatcg aactgaaaga gtcaggaaaa ccatttaacg gaacagtcaa
     8221 attattagcc actgtcggag aagaagtggg tgaactaggt ggggaacagc tcacgaaagc
     8281 aggctatgta gatgacttag atgccttgat catcggagag ccaaccaatt acagtttgat
     8341 gtatacgcac atgggatcaa tcaactatac agtcacctcg cacggaaaag aagcgcatag
     8401 ctctatgcca gaccaaggtt ataatgcaat caatcaccta aatgaattca ttacgaaagc
     8461 aaatgctgaa atgaatcacc tcgctgaaac aatcgagaat cctgtcctag gaaaaacaat
     8521 ccataatgtt acattgatca gtggcgggaa tcaagtcaac agcatcccaa gccatgcgca
     8581 gctccaaggg aacattcgat cgattccaga atacccaaat gataaaatca ttgcattgct
     8641 gcaatcaatc gtcaatgaat tgaatcaaga gacagactat catttggaat tgatgatcga
     8701 ttacaacaaa atacctgtca aagctgatcc agattctcct ttgattcatt gcattcagca
     8761 acaattcagc cagccattac ctttagttgg agcggcagca acaacggatg cagccgaatt
     8821 tacgaaagct aaccattcct tcgactttgt cgtctttggg cctggtgtcg ttactttgcc
     8881 tcatcaagta gatgagtatg ttgaaatcga taattatttg gatatgatcg aaaaatatca
     8941 aggaattatt ttatcctact tagcgtaaga tttttttaat aaatgaaaaa acagagccag
     9001 attagcatta ttttgctaat ccagctctgt ttattcagcc tatttttctt tttgaatata
     9061 tcaagcgttg tggcaacata gtgatgacta atgaaacaac gacgatagac aacaaacgat
     9121 caaggtagtc tgtacctgcc tgtaccaaga tcacacttgt tgtctgactt aaacctaacc
     9181 catgtaatag ctgaacgatc atactggatc ctgaggaggt gatcccgcca aataactgaa
     9241 cggtaatgat cgaagaaacg atcgttccag gtaccgacaa aatcaaagcg agtaataaca
     9301 gtttcaagct gtttttatct ttgccgagat atcttggcaa aatcaatcca gcaagcaagc
     9361 ctgtgagaat ttggattggc gaataataca gagaaaaaat atctgtagtc actccactta
     9421 aaataccgct ggacaatcct gtcagcattc caggtacagg cccaagcaat gcaccagcaa
     9481 aaatcgttcc gatagaatcg agatagattg gcaaacgaag ccataaagca atcgttcctc
     9541 caatataatt gatcccaata cacaaagcca tcactgtaag tacgcgagtt gccattttgt
     9601 ttttattcat tttgttagcc catctttaac ctttccaaat ctttcataat cgtttcttct
     9661 tgtgcattta aaagaacggt taaaaacttt cggaaaaaaa gacgagtatc tacttttgtc
     9721 ataattttac tgttagcagg tttctgccaa aattcataac gatcaaccaa tgtttgtcca
     9781 cgacttattc cttgagtttc cacagcagtg aagcttttga aaccttcaca gatgggctcc
     9841 tcgataaaat aagcaacagc taacggatca ttgatgacac atcctaaaat tctttcttgt
     9901 ttccagtgga agtcaaaata aaaacgagtg atcgctttta gatactctcc ctcttcagga
     9961 ttcatttgac agcagtattc gaggatagtc ggtgtcaaga cgatttctct tgtcacatct
    10021 aacccaacca tttctatcgt ttgtttgaga ttctcgaata cataagaagc tgcttcagga
    10081 tcacaccagt aattgtattc agcaactggc gaacagttac catggctttt gtaagttcca
    10141 cccatcgaga caaaacgttc catatgtttc ccaatatttg ggttgacttt caaagcagaa
    10201 gcgatattgg tcaaaggacc caaagcaatg actgatgtat ttgtcttttc tttgaaataa
    10261 tcagccaaaa aatcaacggc atggagaggt tctgcctgtt ttctcaagat catcgggaaa
    10321 tttgtttccc ctaaaccatc cataccatga gtgtcttgtg cactgatgaa aggttgcttc
    10381 aaaggttttt ccattccttg ataaacagga atgtctaaac gatcacagcg ttccaggcat
    10441 ttcagcacat tctgcgtacc tatatctgca gggacattcc cacaaactac ggtaatagca
    10501 actacttcga tatcagggga tttgacagct aatgataaag ctaaagtgtc gtcgatccct
    10561 ggatcgcaat caataattac ttttcgcatt atttccaatc tcctttcttt agagatactt
    10621 agttttttat tctgggaagt ttccgaacca gtccaagacg agcgtcttga ttataatgca
    10681 cgaaattagt atactggctt ttattgcgaa tgtaaatgct ctcttacaag taaatctatt
    10741 gaggagaact ttaagtaaat gatacaatga aaaagaaatg gaatttacgt gtgccgaaaa
    10801 taaagaatcc tatggatcgg tacattggat aatagataga ttatagtttg gcagaaagcg
    10861 gcgggtgtac aatggttatt ttttatttca ttcttattct gataattata gggatcagtt
    10921 accattttct ttcttatttt tatgcaaaga aattagtaag agcgggagta cagagaggat
    10981 atgactggta tgaagacact ggtcatcgtt tgatggaccc tgctgcagtc acgtcagtag
    11041 aaaaaaaacg acaaaaatta gaagaagagt gtgaaagctt ttgggagcag ggaacaccca
    11101 aaacaatcaa aagttatgac gggttgaaac tagctggtca gatgttcatc catccaaata
    11161 agcagaataa atgggtcatt tgcgtacatg attaccgaag cacagggaaa agagacatgt
    11221 ctcatatagg taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg
    11281 cgcacggaga aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa
    11341 ttgcctggat ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg
    11401 gatcaatggg agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca
    11461 aaggatttat tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa
    11521 gtaaaatcac tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga
    11581 aatacgccgg ttactcctta aagcaagcat cagctacgag acagcttggc agcaatcatc
    11641 tgcctttatt agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca
    11701 cgattcaaaa tgctacggca ggggataaag cactgctgct tgttccggaa gcagaacact
    11761 tggaagcatc gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac
    11821 aaagaataac gatgaattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat
    11881 tgtttataga tcaggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga
    11941 ggagtgcggc atatggaaat caaagaagaa aaaatcgtct tgctttattc aatgatgaac
    12001 aacaagagat cggagaaatg acatggtcag atgcgggccc agacatcatg atcattgacc
    12061 atacatttgt agatccagca tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg
    12121 gagtagaatt agcaagacga gacgggaaaa agatcattcc tttatgccct tatgcaaaaa
    12181 aagagtttga aaagaaacca gaatatcaag acgtcttacg tcagtcataa gaataacagc
    12241 tgaaagtttt gaactttcag ctgttattcc atttatatag agttgtcacg gacttttatc
    12301 tatgataaag ttaaagagac gatatctttt gggaaggagg ctgcgatatg tataatttaa
    12361 ttttgggaat cgtgatcgtt ttatcgattg ttatggtcat tgcgattatg atgcagccaa
    12421 gcaagcaaaa tagtgcagcg agtgcattta ccggcggtgc ggatcaacta ttcggcaaac
    12481 aaaaagctcg cggctttgaa gctgtcatgc agcgatcaac tgcagttatg ggagctatct
    12541 ggatgatctt attatttgtc ctagcatatt tatcttcgaa gtagttgaat aagcctctcg
    12601 caaaacggcg ggcggctttt tttattgcaa agagaacgct taagaagaag ttatgaaaga
    12661 aactatggta aaatgaggcg aaagaggtga gacgagtgag acaattacca aaaccccttt
    12721 atgcaaaaca tggaaagcga gcggttttgc tgcttcacgc ttactcagga agtccaaacg
    12781 atgttcgaat gctcgcacga tttttggaaa aagcagatta tacagtttat gcgccgcttt
    12841 ttaaaggaca cggaacaatg gagccatatg atatcttgca agaaaaagca gaaagctggt
    12901 gggcagacac taaaaaagcc atccattttt tacagtctga gcagttttct gatattgccg
    12961 tattaggatt atcaatgggc ggcatctttg ctgtacgagc actagaagaa gaatcagtga
    13021 ttggcggcgg ttttttctgt tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact
    13081 ttgaaaaata cgtacgtcaa gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg
    13141 aaaaagcagt tgcttaccgc tcgttagctg aacaacaatt aatggatata caagatcagg
    13201 cagcaatcgt agaaagtagg ttgtcagata tccaacagcc gatattttta gctcaagctg
    13261 ggaaagatga aatgatcgac cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc
    13321 agcgagtgac acttcaatgg tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa
    13381 gacgcgaatt agaaaaagac gtcttggaat ttttagaaaa attaccttgg aatgaggaat
    13441 aaatatgaca aaagaaacga taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa
    13501 acgcagtttt tctatggaag aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt
    13561 taaattacta gtacaaacga ttgctcaaat ggaaagagaa cagagtatag ttttcaataa
    13621 aaaaggaaaa gtgaaacttc ctttaaaacc aatcttgatc gaaggaacat ttcgagcaaa
    13681 tgaacgagga tttggatttg taacgattga tcccgaagaa gatgacatct atatcccaaa
    13741 agaagcgact ggttttgcaa tggatggaga caccgtagca atcgatatca tgaagcgagc
    13801 aaatacagat atggatcgcg gagcagaggg gaaagtagtc gagattcgag accgggcgac
    13861 tactcagtta gcaggggaat tcgttgctta ttcagaagaa gaaatacaag aaacagatct
    13921 ttacggcgtt gttatcccga aagatagaaa gctcaaccaa tttaaagtat atgcagcagc
    13981 agagggagtt cgtccagttg acggaagtat tgtgctgatc gaactgaccc attaccctga
    14041 aaaagggtat gcgactagtt tagaagggat aatcaagcaa gtgattggtc ataagaatga
    14101 ccctggcatg gatatcctct caattgtcgt ggctaatggt atccctacga aatttcctga
    14161 tgatgtattg tctgaagcag atgaagttcc agatcaaatc agcgaaaagg atataactgg
    14221 acgtagagat ttaagaaatc agctcattgt tacgattgat ggagaagatg caaaagatct
    14281 agatgatgca gttacggtta aaaaattaga taacggtaat tattttcttg gagtacacat
    14341 tgcagatgtt tcttactatg tgacacaagg cagtcaattg gacagagaag catatgaaag
    14401 agggacaagt gtatatttga ctgatcgagt aattccgatg atcccgcaac gtttatcgaa
    14461 tggtatctgt tcactgaatc cccatgttcc aaggctgacc atgagttgcg aaatggagat
    14521 tgatccaaat ggacaagtag tctctcatga tattttccca agtgtgatcc aaacaacaga
    14581 acggatgacc tacacagctg tcaatcaaat cttagaagat caagacgaag aaacgatgca
    14641 acgctatgct cagctcgtac ctatgttcca agaaatgggt gaattgcacc gaatccttga
    14701 aacgatgcga atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt
    14761 tgatgctgaa ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt
    14821 gatcgaatct ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa
    14881 tctgccgttt atttaccgaa tacatgaaca gccaaaagaa gaaaaaatgc agcgcttctt
    14941 tgattttgct tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa
    15001 agatttacag catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac
    15061 tatgttgcta agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg
    15121 cttagcggca gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat
    15181 cgtccatcgt ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg
    15241 ggctgcggaa cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga
    15301 aacagaacgt gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga
    15361 agaattcgat gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa
    15421 tacagtagag ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga
    15481 aaatcattta gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacagaaagt
    15541 gcgagtaaaa gtagtcaaag ctgatccaga tgaacgagca gtagattttg aacttgtttc
    15601 cgcagaagag gttacagcgt tggaacggcc aaaaacacga aatggaaaat caggaaaacg
    15661 cgaaggaaaa caagcaggaa aacgcccaga aagaaatggc aagaagaaca aacgatatag
    15721 cgatagtaag caaacgttcg gtaagaaaaa agggaagaag ccattttaca aaggtgttaa
    15781 aaaaaagaaa aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg
    15841 aaaattaatt gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga
    15901 agcagggatg gtcttgcaag gaaccgagat caagtcgata cgaaacagcc ggatcaatct
    15961 aaaagatgga tttattcgcg tccgcaacgg agaagctttc ttgcataatg ttcatatcag
    16021 tccttatgaa caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt
    16081 acacaaaaaa caaatcatcc ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt
    16141 tcctttaaaa gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcgaaagg
    16201 gaaaaaatct tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga
    16261 tcgaacatta aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt
    16321 ttgcttttac ttaatgataa aatttgttag catttaatct gataaaacat gattatcata
    16381 ggttttttct tagatcagga gcaccgtgta taaagaaaaa ttcgcttcgt ttttgactga
    16441 aataaaaaag tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg
    16501 ataccgtgtg gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg
    16561 tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg ttccacattg
    16621 gaccaatttg gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt
    16681 tcttcttagc ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg
    16741 cgatagaatg ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag
    16801 aattgagcaa tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca
    16861 acatcggttt agtaacaaaa atcgttttac ctaacgaaac aacactttgg aaaagcccga
    16921 cagcagaccc gtttgttact ttaactttgg catttataat gatcttactt actcacttat
    16981 ttggggtgaa aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccatata
    17041 gttttatgtt tccgatgaaa ctgattgaag aatttacaaa cttattaaca cttgctttac
    17101 gtctttatgg gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatggtaa
    17161 taagcttagg ctggttctca ttgccgttag caattccatt agagatggtc tggatcgcat
    17221 tctccttgtt tatcggaagc atccaagcat ttgtcttcgt tactttatct atggtttata
    17281 tgagccataa gattgaagtg gaagaataat attcgtttta aaaacaaact actatttatc
    17341 taatttagga ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca
    17401 gcaatcggag ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct
    17461 cgccaacctg aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtc
    17521 gaagctgttc ctatcttagg ggtagttgtc gccttgattc tagtttttgg tgtttaattg
    17581 aatcagtatt aaggcagaaa caatttggtt tgttactgct tttttacatg aaaactagaa
    17641 gtacttcaaa gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc
    17701 aatgcgatgt taggaaatgt catcgttgtc agcggctcat ttttgatact actggctctc
    17761 ttgaagcatt ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc
    17821 gccaatgatt tggattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa
    17881 cgcgaacaac aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa
    17941 gaaagtggag aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt
    18001 ctaaaaagta aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta
    18061 aaagacgatg ttgcagaact atctcttcaa atcgcggaaa aaatcttgaa caaagaatta
    18121 tctccagaaa tgcacgaatc attaatcaat caatatatcg aaggtctagg ttcttcaaat
    18181 gaaactagat aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat
    18241 cgattcaaat caagcagaag aaatctatca agatctcgta agcttacgtc aaatctatga
    18301 acaagtacct ggattaggta atatgttaag tgatgttcgt ctagaacctc atgaaaaacg
    18361 agaaatcatg gacaagcttg ttagtggcta tgatggaatc gttaaaaact ttttagaagt
    18421 tgtttatgcg tataaccgaa tggatgatct tttgttcatg atcgaagaat atgaacgtcg
    18481 atatgatgaa catcaaggtt tgctgttggg aagtgtcaca acagcagttc cactttcaga
    18541 cgaacaacga atacaattag aaaagaatgt tgcaaaaaca atgaactatc aatcagtcga
    18601 attaaaacaa atcgttgatt cttctattat tggtggcgcg attgttgaag ctaaccatcg
    18661 agtaatcgac ggaagcattc gcacgcaatt agaaaatatg cgtaatcaat taaatagata
    18721 gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta
    18781 aagaacaaat tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt
    18841 atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc
    18901 ttgaattttc aaatggctca tacggaatgg cgcaaaactt agaatcaaat gatgtaggta
    18961 tcattatcct tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggaa
    19021 agatcatgga agttccagtt ggagaggcct tgatcggacg ggtagttaat ccgctaggtc
    19081 agccaatcga cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcaatgg
    19141 caccaggcgt tatgcaacgt aaatctgtta acgaaccaat gcaaacaggt ctaaaagcaa
    19201 tcgacgctct tgtaccaatc ggacgtggcc aacgtgaatt agttatcggt gaccgtaaaa
    19261 cagggaaaac ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct
    19321 gtatctatgt agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga
    19381 aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcgc
    19441 cgttgcttta tatcgcacca tatgctggta ctgcaatggg tgaagagttc atgtacaacg
    19501 gtaaacatgt cttgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac
    19561 tttccttgct acttcgccgt ccaccaggtc gtgaagctta tccaggggat gttttctact
    19621 tacactcacg cttattggaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa
    19681 tgactgcctt accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa
    19741 acgttatttc gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta
    19801 ctcgtccagc ggttgatgcc ggactatctg tatctcgtgt agggggttct gcgcaaatca
    19861 aagcaatgaa aaaagttgct gggactttac gtctagactt agcaagttac cgtgaattag
    19921 aagcatttac acaattcggt tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg
    19981 gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc accgctagct gttgaaaaac
    20041 aagtagtgat tttgtatgct ttgacacatg gattcttaga tagtatccct gtagacagta
    20101 ttttagactt cgaacatgaa ttattcgaat atctggatac caatcattca gatatctttg
    20161 aaacaatccg tacaacaaaa gatcttccag aagaagaagc gttgaacagt gcgattcagg
    20221 aatataaaga catgttctta gctacaaaag gaaataattc ctctacagaa gataaattga
    20281 aatccattca aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag
    20341 acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct
    20401 gcttctaagt tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa
    20461 gtaagagaaa tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat
    20521 cctagagggg atatcaatta caacagcatg ctgctcagcc gtcctgttaa gaaaactggt
    20581 tatatcgtca tcactgcaga tggcggttta gtcggtggat ataacagctc gatattgaaa
    20641 caaacgatgt ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca
    20701 attggcggaa ctggtgcaga cttctttaaa gcacgcggta tcaatctagc atatgagctg
    20761 cgcaatctat ctgatcagcc aagctttgat gaagtccgca aaatcgtaaa tatggcaaca
    20821 actatgtacc aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat
    20881 tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa
    20941 gaagcaacta ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct
    21001 cagttattgc ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca
    21061 gcagaacatg ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc
    21121 attgatgagc tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt
    21181 accgaaattg ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat
    21241 gagttcaggc aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga
    21301 ccaatcctta ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc
    21361 aaaagttgtt cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat
    21421 ggaatcaaca gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc
    21481 tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga
    21541 tttagaaaca cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc
    21601 ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt
    21661 gcttgcccca tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa
    21721 aaccgtactg atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt
    21781 atttactggt gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga
    21841 ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc
    21901 acgtatgcgt gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg
    21961 tcaagatgta ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt
    22021 atcagccttg ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga
    22081 aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca
    22141 agcaatctat gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca
    22201 tttggatgcg acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt
    22261 agatccattg gcttcttctt caagtgcttt agcacctgaa atcgtgggag aagaacatta
    22321 taaagtagca acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat
    22381 tgctatctta ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg
    22441 cagagtccaa ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc
    22501 aggttcatat gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa
    22561 atacgatgat cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga
    22621 aaaagcgaaa acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa
    22681 atgtggtaac tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta
    22741 cgacagctgg tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga
    22801 ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta
    22861 atggcggaat catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac
    22921 gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa
    22981 tcgaagaagc aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc
    23041 accgagcaat caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta
    23101 tccgtgttta agaacagtaa aaagaggtta tgacagaagt ggtcagcttc aagacataag
    23161 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga
    23221 agctacttct ggaacaccgt atatcagagg ttgtaaaatc agcgttttgt cctgagtatt
    23281 agagaaacaa a
//
LOCUS       DACEND010000032        23170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEND010000032 DACEND010000000
VERSION     DACEND010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.033"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW746_001062"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW746_001062"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1077976.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW746_001063"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW746_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1077977.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW746_001064"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW746_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1077978.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW746_001065"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW746_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1077979.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW746_001066"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW746_001066"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1077980.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW746_001067"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW746_001067"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1077981.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IW746_001068"
     CDS             complement(8053..8523)
                     /locus_tag="IW746_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1077982.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IW746_001069"
     CDS             complement(8569..8994)
                     /locus_tag="IW746_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1077983.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IW746_001070"
     CDS             complement(9061..9330)
                     /locus_tag="IW746_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1077984.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IW746_001071"
     CDS             complement(9341..10936)
                     /locus_tag="IW746_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1077985.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW746_001072"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW746_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1077986.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IW746_001073"
     CDS             complement(14489..15049)
                     /locus_tag="IW746_001073"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1077987.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IW746_001074"
     CDS             15402..16376
                     /locus_tag="IW746_001074"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1077988.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IW746_001075"
     CDS             16546..17946
                     /locus_tag="IW746_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1077989.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IW746_001076"
     CDS             complement(18024..18746)
                     /locus_tag="IW746_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1077990.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IW746_001077"
     CDS             complement(18825..19331)
                     /locus_tag="IW746_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1077991.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IW746_001078"
     CDS             19454..21253
                     /locus_tag="IW746_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1077992.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IW746_001079"
     CDS             complement(21321..22895)
                     /locus_tag="IW746_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1077993.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6354 a         5026 c         4228 g         7562 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt
//
LOCUS       DACEND010000033        23126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEND010000033 DACEND010000000
VERSION     DACEND010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.034"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..278
                     /locus_tag="IW746_001080"
     CDS             <1..278
                     /locus_tag="IW746_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1077994.1"
                     /translation="FQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNE
                     EEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            409..1076
                     /locus_tag="IW746_001081"
                     /pseudo
     CDS             409..1076
                     /locus_tag="IW746_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            1078..2502
                     /locus_tag="IW746_001082"
     CDS             1078..2502
                     /locus_tag="IW746_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1077995.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            2520..3089
                     /locus_tag="IW746_001083"
     CDS             2520..3089
                     /locus_tag="IW746_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1077996.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            3199..4953
                     /locus_tag="IW746_001084"
     CDS             3199..4953
                     /locus_tag="IW746_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077997.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            4907..6679
                     /locus_tag="IW746_001085"
     CDS             4907..6679
                     /locus_tag="IW746_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1077998.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            7202..7471
                     /gene="rpsN"
                     /locus_tag="IW746_001086"
     CDS             7202..7471
                     /gene="rpsN"
                     /locus_tag="IW746_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1077999.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            7486..7665
                     /gene="rpmF"
                     /locus_tag="IW746_001087"
     CDS             7486..7665
                     /gene="rpmF"
                     /locus_tag="IW746_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1078000.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            7697..8074
                     /locus_tag="IW746_001088"
     CDS             7697..8074
                     /locus_tag="IW746_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078001.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            8095..8244
                     /gene="rpmG"
                     /locus_tag="IW746_001089"
     CDS             8095..8244
                     /gene="rpmG"
                     /locus_tag="IW746_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1078002.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            8268..9251
                     /locus_tag="IW746_001090"
     CDS             8268..9251
                     /locus_tag="IW746_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1078003.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            9281..9403
                     /locus_tag="IW746_001091"
     CDS             9281..9403
                     /locus_tag="IW746_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1078004.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            9421..10944
                     /locus_tag="IW746_001092"
     CDS             9421..10944
                     /locus_tag="IW746_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1078005.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            10968..11207
                     /locus_tag="IW746_001093"
     CDS             10968..11207
                     /locus_tag="IW746_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078006.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(11400..11861)
                     /locus_tag="IW746_001094"
     CDS             complement(11400..11861)
                     /locus_tag="IW746_001094"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1078007.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(12056..12274)
                     /locus_tag="IW746_001095"
     CDS             complement(12056..12274)
                     /locus_tag="IW746_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078008.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            12392..14008
                     /locus_tag="IW746_001096"
     CDS             12392..14008
                     /locus_tag="IW746_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1078009.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(14249..15589)
                     /locus_tag="IW746_001097"
                     /pseudo
     CDS             complement(14249..15589)
                     /locus_tag="IW746_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15865..16020)
                     /locus_tag="IW746_001098"
     CDS             complement(15865..16020)
                     /locus_tag="IW746_001098"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1078010.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(16212..16406)
                     /locus_tag="IW746_001099"
     CDS             complement(16212..16406)
                     /locus_tag="IW746_001099"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1078011.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(16316..16648)
                     /locus_tag="IW746_001100"
     CDS             complement(16316..16648)
                     /locus_tag="IW746_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1078012.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(17162..17353)
                     /locus_tag="IW746_001101"
     CDS             complement(17162..17353)
                     /locus_tag="IW746_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078013.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(17447..17833)
                     /locus_tag="IW746_001102"
     CDS             complement(17447..17833)
                     /locus_tag="IW746_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1078014.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            18527..19732
                     /locus_tag="IW746_001103"
     CDS             18527..19732
                     /locus_tag="IW746_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1078015.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            19749..20183
                     /locus_tag="IW746_001104"
     CDS             19749..20183
                     /locus_tag="IW746_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078016.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            20800..>23126
                     /gene="esp"
                     /locus_tag="IW746_001105"
     CDS             20800..>23126
                     /gene="esp"
                     /locus_tag="IW746_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1078017.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         7938 a         3365 c         4333 g         7490 t
ORIGIN      
        1 tattccaatc agctggaacc aaatatcaag atattgttga aaaaattaca tcatttgtga
       61 caaaaaagac agtctgttgt ctagataagt taaagactat cggaatgatc aatcaaaatt
      121 tagtctttaa tgaagaagaa atccacttct acatttactc attctacgct acattctatg
      181 atatattgaa gcatagctat tcaaaagaaa agactatgct attaaccaga agattgtatc
      241 aattttctgt tcctgggtgg attgaattac tgagttaatt aaaaaaaagc ttgcattgag
      301 aattgttctc agttatgctt tttgataaga tcgcactgtt atctgaataa cggtgcattt
      361 ttgtcagctt aagataacag tgcgatctta ttttttggag gtgaaagagt gtcaaaaatc
      421 aatccgttaa ccttgattac tttaaatatt tttttccggt cattattttt ttaggaaagg
      481 gaaatatatt tcaagtgggg tgtttcatta ttgctacagt tttagtattg atgtttcgta
      541 aaataaagac atgtatttat ttcatcttga tttattcagt tctgttttta gtaatgaaaa
      601 ttattgatgc tatcaagata gagtttttta ctatattttt tgggacatcc ttatatattg
      661 tatttcgaat gttgccggtc gtaatgatct catggttgtt ggttagtgtc tatactagca
      721 atgaattatt atcctcttta gaaaaaatac atttaccgaa aaaaataatg ttaagtatta
      781 cagtagtgat cagattcttt ccgacttatc gttctgaaat aagaatgatt aaagaatctt
      841 taaaaatgag aaacattcgc ttgtcttttt ttcagcctct gcaatatttg gaatttctgt
      901 tagttcccgt tcttatacgt gcaactatga ttgctgaaga aatgaccgct aatgcaataa
      961 caaaggggat agaatctcct gtgaaaagaa gttcttacta taaaacaaac atgagcttat
     1021 tagactatat catcctgttt tccgttacta tttttttctt aagtattttg atttgaggtg
     1081 aaagaattgt ttgaatttaa caatgtttct ttttcatata aggatgttaa ggaacaagcg
     1141 cttgttgacc ttaatttttc aataaaaaaa ggcagtctta cattgattac tggttcttct
     1201 gggagtggga aatccacact attaaagtta atgaatggac ttattccaag actttatgac
     1261 ggaaaattgg ttggggaaat tttatttgaa aataactcgc tacaatcctt aacacaagaa
     1321 agtatatcaa aaaatattgg ctacgtaagt caaaatccta gaggacaatt ctttacaaca
     1381 aatacaacga gtgagttagt attttcgatg gaaaactttg gatttacaaa agagcatatg
     1441 gataaaagaa tcaatgaatt agctgaatta ttagatcttt caaatatatt agataaaaac
     1501 attttttcgc tatcaagtgg tgaacggcag aaaatttcta ttggatgtag tttatcgcta
     1561 aactctaaat taattatatt ggatgaaccg tcttcaaatt tggacttcaa agcgactaag
     1621 aaacttgctg acttactttg tgatttaaaa aataaaggat ttactattgt tatagctgaa
     1681 catcgttttt actatttgaa agaattaatt gatcaagttc tatttgttaa aaatagaact
     1741 gtaaaaacta caactattca gttgttaaaa actgaaaaca ataacgcatt aaggaaattt
     1801 gacattttta atagttcgat tgaagttccc tatatcgaaa aggtaagtgc agatgtatta
     1861 aaagttgaag aaattactta tgaaaatatt ctaaaagaca taaattttga tgtcaaagtt
     1921 ggagatgtta ttggtttggt agggaaaaac ggtgtgggga aaacaacctt actaagaata
     1981 ctcatgaaga ttattcgccc taccaaagga aagataatcg aaaataaaaa aaatttgtca
     2041 tcgccgtttt tagtaatgca agaaatggat taccaatttt tcacagaatc agtgaggagc
     2101 gagttagccc tggggaatga aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa
     2161 atggaacttt ataaaatgaa agatcaatta ccgtttgatt tatccggtgg agaaaaacaa
     2221 agattattgg tttctatagc ttcattgtca aaaactaatt tatttctatt tgatgaacct
     2281 acaagtgggt tggattatat caatatggaa aggattgctg accttattaa agacttaaaa
     2341 gaaaagggtg cagtagttat agccactcat gatccggaat ttctttataa aacatgcaat
     2401 agaattattt atttaaaaaa tggtggaata aaaaaagata taaaacttag agtagaagat
     2461 tcaactttga tagaacagat ttttaatgac ataacgaaat agaattgagg agaatatata
     2521 tgaaaacaaa agattatatt tttattggtt tagctacagt tatttcccta gtaatttact
     2581 ttttttcaat aatggttagt agtattggtg gggcgtttgg tcacagcata tccccaggga
     2641 tttttggatt attatctgga atcatatttg tttatattag ctataactac cctagaaaag
     2701 gaatttttac tatttacaca attgttttat tgttattttt tacgttaatg ggcggggcgt
     2761 acttaccttg gtttatcagt tcgattagta tggcgatctt agctgatatt attctatctg
     2821 tatttggata tgatcgagca attcctcaag ttgtgtcttg ggttctaatg caactaggta
     2881 gtgctgccgg acagtggatt cccatatggt tctttacaga tcgttttcgt caagactgga
     2941 tagatagagg acaatctgct gcaacaatgg atgcaatgat ccactacgcg gtgggtatat
     3001 ggggaattat ttcagtgtta gttgtagcaa gtttatctat gattggtgta ttgattggaa
     3061 gaaaagtctt aaaaaaatac aaaaaataga acgtagttta ccttctatct gtttgtacat
     3121 ctctaattta cgctattacg gaaagcatta acagttgtaa acgaataaga gaagaaattg
     3181 aatgcggggg aggaaaaaat gaaagtatac aaaaaattat ttagttatgt ccctaaaatg
     3241 aaatactacg gatggttagc cactttattt tctgattttt ctgtcgtatt aacagtctat
     3301 ggatattaca gtatttacaa atttcttcag tctctaatta ttgtaggaga tgaatattta
     3361 gctaagagtt atgcggttca aactgttgct tggttaactt taggagcttt attttatatt
     3421 ttttctggct tattttctca tatccttggg tttcgcttag agactaattt gagaaaaaag
     3481 ggaattagcg gtttaacaga agcaagtttt cgtttttttg atttaaattc ttcgggatat
     3541 attcgaaaga caattgatga caacgcggct aaaacacata tggcaattgc tcacttgatt
     3601 ccagatagtg ctcaagccat agtaacacct gttttatcag ttgccttagg ctttttcatt
     3661 aactttaaaa ttggattggt tctaatcgcc atgttagtaa ttggtgtatt cttgttaaag
     3721 aaaatgacag agaatacaaa ttttattcaa aaatatcaag aatcgttaga tgtgttaagc
     3781 tctgaaacgg tagaatatat ccgcggaatt caagttataa agatatttgg tgctaaagta
     3841 acatcattta aagccttgaa taaagcaatt aacgattatg ctaagtttgc atataagtat
     3901 tctttgagtg ggaaaacacc ttttgttctt tttcaatggc tattttttgg catagtttgc
     3961 attttgatag ttcctataac cttttttata acttctctta ccactcccaa gatcttagct
     4021 gttgacttgt tgatgttgtt ttttttaagt ggaattattt ttgtttcttt catgagaata
     4081 atgtatgtaa gtatgcactt gtttgaagca agatatgcag tcgaaacact agactccctt
     4141 tatttagaaa tgaagcaaga ccaactaaaa tatgggaacg agacacagat taaaaattgt
     4201 gacattgcat ttgaaaatgt cagcttttca tacaaagaag agtgtgtttt agaaaatttc
     4261 aacttagttt tagaacaaaa aagaatttat gcattagtag gaaaatcagg aagcggaaag
     4321 agtacgatag ctaaactaat ttcaggtttc tacaatgtgg ataagggaca gattaaaatt
     4381 ggtggtaagc ctttaaatca ctattcaaaa gacacaatta ttaacacaat tgcttttgtc
     4441 tttcaagata cgaaactatt caatcagagt atttacgata acgtttctat agcaaatcct
     4501 caagcgccag caagtaaagt atttgacgcg ctaagattag ccggttgcga atcgatttta
     4561 gaaaaatttc ccgaaagaga gcatacctta attggtagta aaggagttta tctgtcagga
     4621 ggagaaaaac aacgtatagc tattgccaga gcaattttaa aagatgcaaa gattgttatt
     4681 atggatgaag ccagtgcatc tattgatcca gataatgagt atgaactaca aaaggctttt
     4741 caatatttga tgaaagaaaa aacagttatt atgatagctc atcgattatc cagcattaaa
     4801 tctgtagatg aaattcttgt cttgtcagaa ggaaaaatag tagaacgtgg aaatcataat
     4861 gaattaatgg ctaaacatcg agtgtataaa caattggtca ctaattatga gaatgcaaat
     4921 gattggaggc ttgacaatga aaaattttta taaaaaaaaa tttgctttaa ctgatcaggg
     4981 cgcagaagct ttaactaaag cttctatttc tagttttttt gtatactgca ttaatatggt
     5041 accagcattc attataatga tgctgataga cgaattagtt ttagaaaatg caaagccccg
     5101 ttggctttat tttgcagtat cgtttgttac tttgcttttc atgtattggt tgttagatag
     5161 ggagtatgaa aacttatata atagtactta taaagaaagt gcgcatttaa gagtgcaaat
     5221 tgcagacgat ttgtcaaatt taccattatc ctatttttca aaacataatt tatcagattt
     5281 atctcaaact atcatgtctg acgttgaagg tattgagcat gcgatgagtc atgcaatacc
     5341 taaagccggc ggtatggctc tgtttttccc ttttatttca gtgatgcttt tggttggtaa
     5401 tgtcaaaatg ggattagctg ttattttgcc aacgttattt agttttgtct taatcttgtt
     5461 atcaaagaaa tcccaaacga aagccaatac taaatattac gatactttga gagaaaactc
     5521 ggaagaattt caagaaacta ttgaattgca gcaagagatt aatagcttta atctatctaa
     5581 aaaagttcaa gacagacttt tcaaaaaaat ggaagagagt gaaaggattc atttaaaggt
     5641 agaattaagt actttttcag tcatggcctt atcctctatt ttctcatatg ttagtttagc
     5701 agtagtcatt ctagtaggtg ttcacttact gttaacgggt gaagtgacta tactctacgt
     5761 cgttggttac ttactagccg caataaaaat aaaggattcc ttcgattcta tgaaagaagc
     5821 agtgcttgaa atattttatt tagctcctaa aatacagcga attagagcta tgaaggaaac
     5881 cagtatccaa gagggatcag atagtcctct gaaatctttt gacattgagt tgcgagatgt
     5941 ctctttctca tatgataaca ataccccaat tttagatcat atctctttta cggctaaaca
     6001 aggagaagtg actgcgttag taggtgctag tggttccggg aaaactagta tcttgaaatt
     6061 agtgtctaga ctgtatgatt atgatgaagg ctgtatttta attgatgggt atgatattaa
     6121 acgtgtatct ccagcttctt tgtttagcaa aatcgctatt gtttttcaag aggtaacttt
     6181 gtttaataca agtattttgg aaaatatacg tattggaaat agtcaagcgt cagatgagga
     6241 agtgaaaaaa gcagctcgtt tagctaactg cgaagacttt attgaaaaac tacctgacgg
     6301 ttatcataca ttggtcggag aaaacggaag ttcactttct ggaggagagc gacaacgcct
     6361 atccatagca cgggcttttt tgaaaaatgc gccaatttta attttagatg aaattactgc
     6421 cagtttagat gcggaaaatg aaaaacgtat tcaggaaagt ttaaatcgtt taatacaaga
     6481 taagacagta ctaattatat cgcatcgctt gaagtctatt gaaaaggtaa ataaaattgt
     6541 agtgatggat caaggaaaac tagttgatca aggtactcat aatgagttgt atcgaaggtc
     6601 agaaatatac aaaaatctca taaagaaaac taaactatct gagaaattcg tctatgaaaa
     6661 agaagctcaa tcacgatgat gaaataggct ttaaattaac ctacaatttt tgggatatag
     6721 ttataattct ttttcaaaaa gaataatttt attaaacagc atctaatttt gaatacacga
     6781 aagaatcagt atgattgaat tagttcactt aatgtctatt cttataatat aaggaataat
     6841 caaagtttta aaaaattggc gataaaccag ttaaacataa aagattgaat aaaaagttat
     6901 agtttctttc atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt
     6961 tgattggact aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa
     7021 atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg
     7081 agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg
     7141 tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta
     7201 tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta
     7261 tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc
     7321 aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg
     7381 gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt
     7441 gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca
     7501 agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca
     7561 gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa
     7621 gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt
     7681 ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta
     7741 ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga
     7801 gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt
     7861 caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat
     7921 ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt
     7981 tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc
     8041 acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt
     8101 caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat
     8161 aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg
     8221 gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag
     8281 ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc
     8341 aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg
     8401 ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat
     8461 gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa
     8521 tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc
     8581 cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg
     8641 atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag
     8701 cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt
     8761 tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc
     8821 gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta
     8881 atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc
     8941 atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa
     9001 aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt
     9061 atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg
     9121 taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc
     9181 taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg
     9241 aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc
     9301 tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat
     9361 tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga
     9421 atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc
     9481 tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca
     9541 acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat
     9601 ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca
     9661 aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca
     9721 aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg
     9781 attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat
     9841 gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc
     9901 aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa
     9961 aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg
    10021 aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga
    10081 ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta
    10141 gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat
    10201 gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg
    10261 aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta
    10321 atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt
    10381 ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat
    10441 gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta
    10501 aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca
    10561 gctgccgagt acaaagatta ttatactact ggctataaga cagatattga tactatcaac
    10621 attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa
    10681 tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt
    10741 gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt
    10801 gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc
    10861 tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt
    10921 gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag
    10981 tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc
    11041 aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat gttagtattg
    11101 acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg
    11161 gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg ataataccag
    11221 agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca
    11281 aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt
    11341 tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc
    11401 tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca
    11461 ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta
    11521 actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca
    11581 tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg
    11641 gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa
    11701 gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca
    11761 tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg
    11821 acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta
    11881 ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg
    11941 ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc
    12001 gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga
    12061 atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt
    12121 gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa
    12181 tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc
    12241 gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct
    12301 cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag
    12361 tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac
    12421 tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg
    12481 aaaggaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt
    12541 cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat
    12601 ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga
    12661 cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc
    12721 aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc
    12781 cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat
    12841 tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt
    12901 aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac
    12961 caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat
    13021 taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat
    13081 agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca
    13141 tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct
    13201 tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc
    13261 aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg
    13321 tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg
    13381 tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga
    13441 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
    13501 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
    13561 tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
    13621 tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct cttttattcc
    13681 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
    13741 catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
    13801 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
    13861 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
    13921 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
    13981 agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
    14041 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
    14101 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
    14161 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
    14221 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
    14281 ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata
    14341 aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta
    14401 ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc
    14461 gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca
    14521 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa
    14581 cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata
    14641 gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
    14701 gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct
    14761 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag
    14821 cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
    14881 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt
    14941 cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
    15001 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
    15061 agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca
    15121 aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
    15181 gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt
    15241 gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag
    15301 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt
    15361 tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt
    15421 cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaag
    15481 caaaacatgt ttcgtcattg atgcgctctt tgcgaaaggc atcctcatga aaggtaagat
    15541 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
    15601 actttgaatg gtttttgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta
    15661 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
    15721 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
    15781 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
    15841 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
    15901 attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag
    15961 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
    16021 ttctccttaa taaatttgta atggacggac gacaatctct ttttttatgt gtgaatctac
    16081 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
    16141 gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc
    16201 aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa
    16261 gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca
    16321 acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg
    16381 tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt
    16441 caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt
    16501 tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt
    16561 cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt
    16621 taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat
    16681 agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga
    16741 agttgataga taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca
    16801 aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg
    16861 cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac
    16921 ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta
    16981 tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct
    17041 atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca
    17101 aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga
    17161 gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat
    17221 aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc
    17281 ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt
    17341 ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg
    17401 aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt
    17461 tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca
    17521 gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt
    17581 gttcaggttc tttcaacaaa ggaaggattt ttgctaattt tatatcttca acaaggagag
    17641 tcacatttaa aacattaatc ggttgtttaa attttatagt atctttagta aacaaatatg
    17701 gtgggagttg atcaagttgt tcatgttttt gaatttcttc ttgtttttta taataagtat
    17761 tagcggtgtt caaaatgacc ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt
    17821 cgttatctga catattgcac acctcctcca ttaagattta aagggaaaag aggcaaaaaa
    17881 cacgaaaaga agctaatgca attatgctgc gctgcgaata acttctataa gctcacatgc
    17941 attgatagga ctgctgatgt ttattttcaa aaatgttttt ttagttgata atggatagtt
    18001 atctttatac ttagaaaaga aaattcatat atcttcttaa atatcaaaca tgtccttatg
    18061 gctaagaaaa tgtcttgagc tttttacgta taataaatct tttctaaaac ctccttttgg
    18121 ataatattct tattcgtgta tagattgtat tttttatttc ttcccccttc ataattaaca
    18181 actagaaaag agttcgaata gacacgtatt tgaacgaaaa tttttattgg attcccaaat
    18241 tggaatattt agaaacgaaa aattcctatt atctgtatag tgcttagata ataacggatc
    18301 ataataatta ttgtctttga actagatata tgtaaatagt attcatcatg atgaaaaatg
    18361 atgagttctc ggtgataaaa tgtacttttt acggtggaat atacatgaaa gcgataagtg
    18421 gatattttat aatgaagtaa gttagcttat tttgacagag gaatagtatt ttagataacg
    18481 tataaaatac tatttaatga tgtagaaagg aaagagagaa acaaaagtgt tttcagataa
    18541 agaagtctta ttaaagtctc ttcatgtttg tttaaagtta cctatcgtgg tttttaattc
    18601 cgaattcagt attataaaag agtatcgctc agatagaact acttattttt tttatgatta
    18661 taaaaagtta ctaactgata atattaaaaa aactaaagat ttccatttct ttactggtga
    18721 ttttaatgag atgtttcttc tatacatgtt taaaaccaga tattacttat ttggtccatt
    18781 tagagcaaat aatattgata aagttttttt taaactaaaa atgaacaatt tgaatgttgc
    18841 aatggctgat agagaacgac tctataattc attgcaaaat ttgactttgt attctttagg
    18901 agacattcga gatattctga ttttagttca ttattttttt actggaaaaa tagaagatct
    18961 attccatgaa ccgttaattg aatatacaga aaatctaagt aaaactatcg agcaaatcaa
    19021 aatagataat ctgttatcac aaaattacga tccagaaata tatctgtttc tttatgaaaa
    19081 taaaatactg gaatatgtta aaaatggaga tattcgaaat ttagaaaaca tggtttttaa
    19141 tctgagtaat ggaataattc caagtgttag tggagatacg attcggtctg aaaaaaatta
    19201 ttctatcatt gtttttgaaa agttagcaca aacgtctata acattaggaa tggatataat
    19261 agaagcgtat caaagtagag atgctttgat acaagaaaac gaattagctg tatcgttacc
    19321 tgaggtctta aaagtgaggg attctgggat tgtttattat actaaggaaa tcggaaaaac
    19381 taagatagag catctttctc ccttaatttc ttctgtcgtt caattcatcg gcttaaatat
    19441 ctataaaaga attactgtaa aggaaatagc taattatttt tctgtaagtg aaactaaatt
    19501 aagagaaagc tttaagaatg aaatgcatat tacgatttat aattatatct ctaagcgaaa
    19561 aatttctacg gctaaaataa tgttgaaatc aaaccatact atcagtgaag tttctttagg
    19621 tttaggattt tcagattctt cacacttttc gagagtattc aaaaagtacg caggtgtatc
    19681 tcctaagcaa tatcaattag gtttagtgga caatttcaac aacattagct gagaggtttt
    19741 attttattat gagactatat catttacgta gtttggagga gttgaggaaa ttggatttta
    19801 acaaaaaatt tttttgggaa atttctgatt tgagttgttt cgtagaatct gagcgtctaa
    19861 gtaatggtaa taatagtgtg ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa
    19921 gtttatgtga agaagctaga gaatctatca gtcatattaa tggtagcaat tttaaacaaa
    19981 cattatttaa tctgattaag ctagatgcta aaatatcaag ttatttattc tttttgatac
    20041 atgatgaatt tatggattat caaaaattgg atcagttaat tgaaagagaa agctgggaag
    20101 attattacgt gggtctaact ttttcagaaa agttaaacaa ctacaagtta atagattata
    20161 agtatttgag tgaaagcaat taattattca tgtagagtac tagacacttt aaatatggac
    20221 aattcaatta taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt
    20281 tgtttcgcta aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta
    20341 ggaatcattc agaaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa
    20401 attccgaaga aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg
    20461 cgggataagg ctccttattc cgttatttga atactctgaa gtttaatcta agaaagggta
    20521 atattttgaa aacatgcttc atcgcataga aatgaactta atttcagaaa gaatacatac
    20581 atatcttttt atatgttttc agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa
    20641 catgaagaaa tgtacttttt ttgttgtaat gttcatgaaa ttctctaagt aaattagtta
    20701 acataaggtt agtaagattc ctattctgtg aataatacgt atcagattta tcttttattc
    20761 ttactaggaa agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag
    20821 agagtatttc tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt
    20881 gttggggttg catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt
    20941 aaagcacaag atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa
    21001 gtaggtgaca aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat
    21061 tataactttt cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc
    21121 aatacaattg gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg
    21181 gaaatccgtc ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat
    21241 ccaaagaaag gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt
    21301 agacctacaa atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg
    21361 gcagaaacaa ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta
    21421 gatgaaattt ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga
    21481 atctacttgg taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc
    21541 tactttttaa cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct
    21601 gaccagttta aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa
    21661 gatggttcag gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa
    21721 aattataatt tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa
    21781 attgatgaac gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac
    21841 aaagtaacat atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag
    21901 cgacgggaag gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa
    21961 cttatcgatt ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg
    22021 actaagccat tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat
    22081 aaaccatttg gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta
    22141 atccagaaca cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac
    22201 cctgacgata aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa
    22261 gaagtatatg atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga
    22321 acaggtcaca aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc
    22381 gctccaaatg aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt
    22441 gtagattcaa gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt
    22501 tttgataaac cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa
    22561 caaaccgtgg tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac
    22621 ttacctgaag atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat
    22681 agtacgaaag gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag
    22741 tttgctgtaa aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca
    22801 actccagaaa cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta
    22861 gctaaagatg ccttttcaat ttcagatgtc tataataagg acttcggtaa tgcaagtgtt
    22921 gatgcaaaca ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg
    22981 attactggaa caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat
    23041 ttagcagtaa ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc
    23101 taccacaaag taaccttcac cgcagg
//
LOCUS       DACEND010000034        23037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEND010000034 DACEND010000000
VERSION     DACEND010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.035"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IW746_001106"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IW746_001106"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1078018.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IW746_001107"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IW746_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1078019.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IW746_001108"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IW746_001108"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1078020.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="IW746_001109"
     CDS             4798..5496
                     /locus_tag="IW746_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1078021.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="IW746_001110"
     CDS             complement(5664..8303)
                     /locus_tag="IW746_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1078022.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IW746_001111"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IW746_001111"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1078023.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="IW746_001112"
     CDS             10146..11321
                     /locus_tag="IW746_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1078024.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="IW746_001113"
     CDS             11325..11966
                     /locus_tag="IW746_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078025.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="IW746_001114"
     CDS             11963..12880
                     /locus_tag="IW746_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1078026.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="IW746_001115"
     CDS             12877..13548
                     /locus_tag="IW746_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078027.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="IW746_001116"
     CDS             complement(14089..14577)
                     /locus_tag="IW746_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1078028.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="IW746_001117"
     CDS             complement(14778..15044)
                     /locus_tag="IW746_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1078029.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="IW746_001118"
     CDS             complement(15044..15307)
                     /locus_tag="IW746_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1078030.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(15300..15572)
                     /locus_tag="IW746_001119"
     CDS             complement(15300..15572)
                     /locus_tag="IW746_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078031.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            15783..15968
                     /locus_tag="IW746_001120"
     CDS             15783..15968
                     /locus_tag="IW746_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1078032.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="IW746_001121"
     CDS             complement(16110..17933)
                     /locus_tag="IW746_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1078033.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..21236)
                     /locus_tag="IW746_001122"
     CDS             complement(18531..21236)
                     /locus_tag="IW746_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1078034.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21542..22489)
                     /locus_tag="IW746_001123"
     CDS             complement(21542..22489)
                     /locus_tag="IW746_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1078035.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22679..22948
                     /gene="rpsN"
                     /locus_tag="IW746_001124"
     CDS             22679..22948
                     /gene="rpsN"
                     /locus_tag="IW746_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1078036.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         6482 a         4871 c         4301 g         7383 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    20041 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    20101 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    20161 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    20221 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    20281 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    20341 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    20401 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    20461 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    20521 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    20581 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    20641 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    20701 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    20761 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    20821 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    20881 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    20941 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    21001 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    21061 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    21121 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    21181 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    21241 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    21301 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    21361 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    21421 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    21481 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    21541 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    21601 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    21661 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    21721 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    21781 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    21841 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    21901 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    21961 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    22021 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    22081 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    22141 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    22201 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    22261 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    22321 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    22381 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    22441 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    22501 ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt
    22561 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    22621 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    22681 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    22741 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    22801 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    22861 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    22921 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    22981 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaa
//
LOCUS       DACEND010000035        22803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEND010000035 DACEND010000000
VERSION     DACEND010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.036"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            223..1002
                     /locus_tag="IW746_001125"
     CDS             223..1002
                     /locus_tag="IW746_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078037.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1048..1992
                     /locus_tag="IW746_001126"
     CDS             1048..1992
                     /locus_tag="IW746_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1078038.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2008..2787
                     /locus_tag="IW746_001127"
     CDS             2008..2787
                     /locus_tag="IW746_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1078039.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2799..3497
                     /locus_tag="IW746_001128"
     CDS             2799..3497
                     /locus_tag="IW746_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1078040.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3525..4289
                     /locus_tag="IW746_001129"
     CDS             3525..4289
                     /locus_tag="IW746_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1078041.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4334..5023
                     /locus_tag="IW746_001130"
     CDS             4334..5023
                     /locus_tag="IW746_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1078042.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            6250..6930
                     /locus_tag="IW746_001131"
     CDS             6250..6930
                     /locus_tag="IW746_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1078043.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            6959..8272
                     /locus_tag="IW746_001132"
     CDS             6959..8272
                     /locus_tag="IW746_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1078044.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            9078..9845
                     /locus_tag="IW746_001133"
     CDS             9078..9845
                     /locus_tag="IW746_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1078045.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            9872..10873
                     /locus_tag="IW746_001134"
     CDS             9872..10873
                     /locus_tag="IW746_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1078046.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            10896..12182
                     /locus_tag="IW746_001135"
     CDS             10896..12182
                     /locus_tag="IW746_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ1078047.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            12216..12869
                     /locus_tag="IW746_001136"
     CDS             12216..12869
                     /locus_tag="IW746_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1078048.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            12902..14107
                     /locus_tag="IW746_001137"
     CDS             12902..14107
                     /locus_tag="IW746_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1078049.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            14237..15562
                     /locus_tag="IW746_001138"
     CDS             14237..15562
                     /locus_tag="IW746_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078050.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            15579..16709
                     /locus_tag="IW746_001139"
     CDS             15579..16709
                     /locus_tag="IW746_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078051.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            16735..17820
                     /locus_tag="IW746_001140"
     CDS             16735..17820
                     /locus_tag="IW746_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ1078052.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            17843..18658
                     /locus_tag="IW746_001141"
     CDS             17843..18658
                     /locus_tag="IW746_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078053.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            18642..20360
                     /locus_tag="IW746_001142"
     CDS             18642..20360
                     /locus_tag="IW746_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ1078054.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            20386..21222
                     /locus_tag="IW746_001143"
     CDS             20386..21222
                     /locus_tag="IW746_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1078055.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            21227..22660
                     /locus_tag="IW746_001144"
     CDS             21227..22660
                     /locus_tag="IW746_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1078056.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
BASE COUNT         7588 a         3319 c         4921 g         6975 t
ORIGIN      
        1 aatcgattaa ttgttatctt attaatgttt tatttacttt taaatcataa attgccctag
       61 tcaagaaaac agtttgtggt atgattatat agggaaaact tgtttacaaa tatttctgtg
      121 aatatattag tttaattatg ttttttaaaa taaaaagctg tcatttttat gtaagtgaat
      181 tatatcttaa tttatctatc atcaatactt aggagtgaat ctatggaaaa gccattcgtc
      241 aagtcattga ctgctgtggc ggtaggtgca ggtgctattg cgatctgttt gtttggttac
      301 catatcaata atcagcggca acatcagcaa cggatcaatt atgcagaatc tgccatcacc
      361 aatcaaaaag acaccttgac ttctttatca aaagaagtag acaagctgta tagtacaaaa
      421 gaaaaaattt ttctaaaccc tgaaataaca gaagaaacaa tcacaagttt gtcaaataag
      481 ctatcctatg tcaaactttc agcagatgat tacgacatca aagaatcaga attgcctaaa
      541 gaagctgttg caatccaaga tgagaagaaa gcaatcgtga cacagttaga tagtgcagaa
      601 tcaaaactga agatccagac agctgtcaac gatttgttta ctaaaaatgt atcaaactgg
      661 cagcaagctg tagacgatgt catcatcaaa gaaaaggtga caagtgcaga tgtcgctcgt
      721 gtacgtgaaa atatgagctt ttttaaagat agtccttgga aaacggttgt tatgcaatat
      781 ttaggattcg ctgatactca gatcgcgcaa gtcacgcaac tagatcagtt attcgatacg
      841 atgttgaagg atggtcaagt gactgccacc gctacttacg atcaatattt gacggcgctc
      901 agtcaaatag agcagatccg gaatgaaaaa ataagcgcag catacgcaac aaaagcacaa
      961 actgttgctc agcaaatggg ctatagcaat ataagttatt gacagttaaa acgttgattc
     1021 aagcaaggtt agcaaacgga ggaaactatg gcaagacgaa gaagaaaatc aagagggaaa
     1081 aaaatatttc tgattatttt agcagttctt atcgtattag tattagcaat tgtaggagta
     1141 ggagcaaaat tatatctgga tctctcacat tctattgaga aaacttacga atcagtggaa
     1201 cgaacccaag gagaacgaac gaaaccagtt gatctggaca aacaagaatc tttctctgtt
     1261 ctattgatgg ggattgatac tggagattta ggtcgggtcg atcaaggaag atcagatacg
     1321 atgatggttg ccatagtcag tcctgaagac aaacagacaa cgatcgtcag cattcctcgt
     1381 gatacgtatg tcgacatcgt aggtcgtgac acaaaagaca agatcaacca cgcttacgca
     1441 ttcggcggag ctgccatggc gatggatacc gtacaaaaat atgtagacat cccaatcgat
     1501 cactatgtct ctatcaatat gaaagggcta aaggaacttg ttgatgcagt tggtggtatc
     1561 gaagtcaata atgacctcac tttctcacaa gatggctatg actttacaat cggtaaaatc
     1621 acattgaacg gggatcaggc attatcttat tctcgtatgc gttatgaaga tccaaatggt
     1681 gactatggta gacaagagcg tcaaagaaag atcgtagaag gtgtagcaaa aaaagtattg
     1741 agtttaaacg gtgtcagcaa ctatcaatcc gttttgaatg ctctagagtc gaatatgaaa
     1801 acagacatga gctttgatga aatgaaaaaa atcgctttag attatcggga ggcatttggt
     1861 acgatcaagc aagatcagct gcaaggagaa ggctttatgc aagataatat ctcctatcaa
     1921 aaagtcagtg atcaagaact tgatcgtgta aaaaaagaat taaaagatca aatgaatcta
     1981 gagaataaat aggaaagtgg agaaaaaatg gaagagacaa tcaacttgga agaaattttt
     2041 gaaacattac gcaaacactt ggtaacaatc atcatctcaa tgtttgctgg cttggccatc
     2101 tcaggaatcg taacattttt catcttgaca ccaaaataca gttcacaagc acaattgatc
     2161 gtgacactgc cacaatcaga cacagcaaat gtcaatgatg tcaatacgaa cctgcaaatg
     2221 atcaatacgt ataaagatat gatcgtcagt gatttagtac taaatgaagt caaagatcgt
     2281 ttagaaacag aagacaatgt gaaaatgact gctggacaaa tcaaagatgc gatctccgtc
     2341 aatcagtcac aaaattcaca aatgttctcg atccaggcaa taagcacgaa tgcagtgact
     2401 gctcaacaga tcgctaacac aacttcacaa gttttccaag agaacgcaaa agacgtgatg
     2461 aatgtcgata aaatctcgat tatttcaggt gcagttgcca atacgacacc tgtttcgcca
     2521 aataataaat tgaatctagt gattggctta gtattaggaa tgatggtagg cgtaggtctg
     2581 gcattcttat tcgaattact tgatcggaca gtgaaagatg aaaaatatgt gacggatgca
     2641 ttaggattcc caatcttagg aacagttcca gaaatgtcag cgaaagaatt gaacgctggt
     2701 atccgtaaaa aaggaacagc tcctgcacga ccaaaagcta caaacgataa aacagcccca
     2761 tcacgcagaa gccgctcaag agtctaggag gaagaaaaat ggcacgaaca cagaaacaaa
     2821 aagcagcaca aactcatgca gtcagtttga tcacattaac aaacccatct tcaccaatcg
     2881 ccgaacaata tcgtacgatc cggacgaata tccagtttgc ctccgcagct ggtcagcaaa
     2941 tcaaaacgat tgtcgtgact tcatcaggtc ctggagaagg aaaatccacc actgcagcaa
     3001 acattgcagt cgtttttgct aaatccggtc aacgagttct actagtcgat gcagatttac
     3061 gtaaacccgt cgtctataag acattccagc tgaacaatgc caggggatta agtacagcac
     3121 ttagttcatc aggaagtgta gcagatgtaa tccaacgaac gccagtcgag aacttgtcga
     3181 ttttgcctag tggacctaag ccacccaacc catcagaatt gttaagttcg ccaagaatgg
     3241 atcagatcct agacgaagca agacagttat ttgacgtggt gatattcgat atgccaccag
     3301 tagtagccgt aacagatgca cagatcatgt cttcaaaaac ggatggcact ttattggtcg
     3361 tacgtgaaaa tacatcacgt aaagaatcat tgaataaagc aaaagaactg ctagagatgg
     3421 tacaagcacg agttttaggt gttgtataca acggagcaga acatagcaaa gatgctggtt
     3481 attactacgg aaattaagga ataagaagag aggagacaag agaaatgatc gatctacatt
     3541 gccatatcct accaggaatc gatgacgggg cagaaaactt agaagcaagt attgcaatgg
     3601 ctgaaaaagc gattcagcaa ggcatcacgc atattttatg cacaccacac cacaataatg
     3661 gaaaatacag caatgaaaaa tcacaagtca tttctctagt cgcttcgtta caagccgaat
     3721 tagagaaaag acagttgcct ttgacgttat tggaaggaca agaagtacgt attaccggaa
     3781 cgctcatcga agacatccac cgagatgaaa ttttatttac agatttagat gatacatatc
     3841 ttctgatcga atttccgaca ctggaagtgc cgttatatgc agaatggctt ttttttgagt
     3901 tgttagaact aggaaagaca ccagtgatcg tccatccaga acgcaacgcg cattttcgag
     3961 aagacccaaa tcatttgctt ccgttcctag atatgggatg tttggcccaa attactgcgc
     4021 caagttatgt tggtgtattt ggcaaagaca ttcagaaaac agccaaaaca atggtcaaac
     4081 ataatctcgt gcagatggct gcttctgatg cccatggggt ctctaagcgt accttttact
     4141 taaaagagtg ctatgagcag attgctaagg actttggtaa ggaaaaagta gtacagatga
     4201 aacaagtcgc aaaggatctg atcaacgggg atgaaatcag ttatccagtc tatgaagagg
     4261 taaaaaagaa gaagtttggt ttattttagt tctttattcg tagataaaaa ggaaatgagg
     4321 agagagggaa agtatggaaa gtgaagattt attatctcgt attagtgaag tacatcctaa
     4381 tcaagtttgg ttggataaag aaaaagttga aaaacgatat atctatttat tttttaaaag
     4441 aatgattgat attgtaggaa gcattattgg tttgatttta gcttctcctt tgatgttcta
     4501 tgtagcttat cgaatcagaa aagaagagcc aggaagtccg atattttttt gccatacacg
     4561 tgttggtaaa aatggaaaaa cattcaaaat gtataaattc cgttcgatgt gtttagatgc
     4621 tgaagaaaaa ttggaagaat tacttgatca aaatgaaata gaaggtgcaa tgttcaagat
     4681 gaaagaagat ccccgtgtca ctaaaatagg taaaattatc cgtgccaaaa gcattgatga
     4741 attgccacag ctatggaatg ttttaaaagg cgatatgagt ctaattggtc cacggccacc
     4801 tttggagagg gaagtagctg agtacacgag atatgatctt caacgcttgt atgtaaaacc
     4861 tggttgttca ggtttgtggc aagttagtga tagaaataat gttagtttcc ataaaatggt
     4921 tgagttggat ttagagtata ttagtcatcg aagtttaatg tacgacttga ggttgatttt
     4981 aaaaacgttt tatgttattg tcaagcccaa tggagcatat tagttaggga tagaatgatg
     5041 gattgttaat aaaaaagatt acacatgttc atattatcgt ttcaaaagta ataactgtaa
     5101 ttatggaggg tttgaatcac ttgtagaaaa tctaaaaaaa gaaaggtgaa tggggcaatt
     5161 cagtatcatt attcttatat aaaagaaaat attcgaaatt ttgggatgat accactctta
     5221 aatataatga tctaaatgct ttaatattgg tatatccgat ataggaactg acgagctatt
     5281 tagtatgata caaaagtata ttgatagcca ttaataaatt ttccaaaaaa taatgacagc
     5341 acaccaattt ttataattta gcttgttgaa tcggtatgtt catgggacgg ttcaaaaaaa
     5401 gaatcgaaaa aataaacggt aaaattttag tgaatcctat gtaataaaat ttggatagat
     5461 ggaaaaacag cacataagga ggctgagttt gttgtcattt ctgctcctac aaactatggt
     5521 agtgaatgtg ttattgaatt agtgatgtct gttacttcag agacattggg gataattaac
     5581 ttcactatac ctgtagggta tacaaaaaat ggaagaaaaa atattatcaa tagattttat
     5641 ttagttcaga atttttacgt ggaagtaaat tattgatgat aatctttatc caattagaat
     5701 tattatcgta ggatgtgatg atgatagccg tgaagttgtt caagtattta ccaatgtcta
     5761 caacgcagaa aattagtttg ggtttttcgg attggaaaat ttcaatgtat aaaatcataa
     5821 atttgctgta aatagacagt ttggggtagt aatagcccaa taaattgttc gataaactca
     5881 tctttggggt gagaatagct caatcattga atttcaaacc tgcctttggg gtgggattag
     5941 aggtcaatat gtacctacat aatacttttt ggaaaatgat ggtgttgaga atgtaagtac
     6001 atttcaatgt gaaattctaa tatagcccca aacgaggtca ataaaaatga cctaaactcg
     6061 tatttctccc ctaaacgagg aggagataga tatgaaaatt gaatgtgccg gcgttggagt
     6121 tggctgcttt tagtaggaat gaacatggtt cgatgagaac tgtttcagat agagtggtcg
     6181 atgatcacag gcctttaagt cgtttacaga tgatgagact tgtaaaaata gagggacgaa
     6241 tggaaaaaga tgattggtat agaagaaaag aaaggtgttc agaaagacgt aacaattgat
     6301 agggaagaga atcttagggg acatagattc tattggattg gacgcagagc acaagatatc
     6361 ttcttctcgg ctctagcttt gattgcattg agccctgtga tgctaattac agcgctcgcc
     6421 attgtgattg acgatcccgg tggcagcccg atttttgtgc aggatagagt tggaagaaat
     6481 ggaaagctgt tcaagtttta taaattccga tctatgtgtg tagatgctga agcaaaatta
     6541 gaagccttgt tggcacaaaa cgagatggat ggtccggtct tcaagattaa agatgaccca
     6601 agaatcacga gagttggtaa atttattcga aagacaagta tcgatgagtt acctcagctc
     6661 tggaacattc ttaaaggtga tatgagtatt attggaccca gacctgcttt accgagagag
     6721 gtggaacaat acggtgacta cgaaaggcag cggttgttcg tacagcctgg tcttagttgc
     6781 tattggcaga ttgctccaca tcgtaatgaa ctgtcctttg aagaatgggt ggaacttgat
     6841 gtgaagtaca tcaatgaaag aagctttatc acggactgga agattatctt cttgacttta
     6901 caggctgtat tatttggata tggtgaatga ttatatatag tattaggagt tgaaatatgt
     6961 gaatattatt ttattatcgg gtggtagcgg taggcggtta tggccgctgt ccaacgatat
     7021 tcgtagcaaa caattcatca aactgtttaa ggatgaaaat ggcgaatatg aatctatgat
     7081 tcaaagagtt tatcgccaga ttacaactgt tgacccagat gcaaaggtca ctattgcaac
     7141 aagtaagtct caggctagtg caatccataa tcagttggga gataaggctt cgatttgtgt
     7201 agaaccttgt cgtagagata ctttccctgc aattttgtta gctgcagcat atcttcacga
     7261 tgaacttggc gtaggtgatg atgaaagtgt tgcggtttgt cctgttgatc cttatgtaga
     7321 taattcctat tacgaaggcg ttgaagccct tgagaagttg gtgcagcaga atacggctaa
     7381 tttgactttg atgggtattg cacctacata tcctagtgaa aagtacggtt atatcatccc
     7441 tgaaagttct aacgaagtaa gtactgttaa ggagtttaag gaaaaacctg acaaagcaac
     7501 ggcagagaaa tatttagctc agaatgctgt ttggaatgcc ggtgtatttg cgttcaaact
     7561 tggttacctg ttggaaaaag ctcacgcaat ggtggatttt gaagattatc gtgatttgta
     7621 ttccaagtat gagactttaa ccaagatcag ctttgactat gctgtggtcg aaaaagaaac
     7681 gagcattcaa gtgatgcgtt ataacgggga ttggaaagat gtcggaactt ggaacatgat
     7741 ggctgaagtt atggctgata atattaaggg gaaagctgta atggatgata cctgtgttaa
     7801 tactcagatt gtgaatgagt tgaatattcc tattcttgct atgggctgca aagatatggt
     7861 aattgcagca agtggcgatg gaattctgat ttctgataaa gagcgcagtg gctatatgaa
     7921 gccgtatgtt gagaaaatca gtacggaagt aatgtttgca gaaaaatcct ggggtacata
     7981 taccgttatg gacgttcagg aaggctccat gactgttaaa gtttctatgc aggccggaca
     8041 gaagttgagc taccactacc acaattctcg cgatgaagtt tggactgttg tagcaggaca
     8101 gggtaaagcg attgtagatg gtatgaagca gattcttcgt gcgggtgatg tggtgactat
     8161 tgccgcagga tgtaaacata caatcgaagc cgtgacaaat ctggatatta tcgaagttca
     8221 gattggtgaa actattagcg tcgctgacaa gtataagagt tctttgaact aaaagttcgc
     8281 gaggtagtta ttatggagat actaaaattt caacccgtgg gtaaagatta tctttggggt
     8341 ggagaacggt ttaagacgga atttggaaaa aattgactta acaccattaa gcttatacgt
     8401 gggaatgctt agttcatcta gacgataact acgtacggaa atatcaaata tgaaggacag
     8461 aatggtaaaa ccgagttgtg gtatgtattg gaagctacac cggggggtga tttatgcttt
     8521 tgagtacgat gtaacagaag aaatgttacg aaatgctgtg atggacagat cacttgggaa
     8581 acatttatat aaagaagaag tacacaaggg agatcttttt tatgtcagtt catggtattg
     8641 gcgctggagt attagtggct gaaatacagg aaagctcaaa tgttacctat cgagtttacg
     8701 attatgatcg agtagataag aatgataata agtgagaatt gtactttgaa aaagcgattc
     8761 aggtaatgaa tatggaagcc actttgaaat tggagaaaag aaataagctc attcattttt
     8821 atcctggtgg ctctcgtgaa atactgtgta gatgtaaata ctgtgagccc gaaagaatcc
     8881 aagtaaagac ggggttttcg ttctcggtaa aagaggaatc ttttcaagtt cttttatgta
     8941 tatcggggga cggacaagtt gagtcggcaa atggtgttat gaaactcatt agatttgtaa
     9001 aaaagatatt gtatgttttt gccaacgaat attggaagat gttaagtgtt gagcgatgcg
     9061 gacttactga aaattagatg gggatagagg gaggatatct aatgtcacga atgagattta
     9121 tgaacacaga aattgacaat cttacaatgg gtgaagtatt ggtagaggtt gaaaaaatga
     9181 ttcaagatag gagatgcgga tatgttgtaa ctccgaatgt ggatcacatt gttcagttgg
     9241 aaacaggaaa agagttgcaa gatgtttata aaaacgcatc tttaattttg acggatggta
     9301 aaccattgat ttggatttca aagttttata agacaccaat aaaagaaaaa atatcgggat
     9361 ccgatatatt ttctttactg tgcaagatgg ctgcagaaaa gggatacaca atgtattttt
     9421 taggtgcggc agagggcgtt gcggctaaag cggcagataa cctgatgaaa agatttccgg
     9481 gattaaatgt tgtaggaaca tactctcctc cttatgggtt cgaaaaagac gaagatgaaa
     9541 tgtttaaaat tgattcccaa attaaacgag caaatccaga tatgcttatt gtgggtttgg
     9601 gttgccctaa acaggaaaaa tttatgtttt ataactgtga tagattggga gtacctgtgt
     9661 cttttggtct aggagctagt ttggactttg aggcagggaa tgttaaaaga gcaccaaaat
     9721 ggatgtcaga ttatggattt gagtggctgt ttagaattac tcaggaccct aaaagaatgg
     9781 caaaacgata tttggttgat gatttaaaaa tctttaaatt agcactaaaa tatcggaaaa
     9841 aataaactat attcaatgag gacaatgaag tatgattatt acaaaaacac cttttcgcat
     9901 gtcttttttt ggcggcggaa cggatatgga agattatttt cgagaaaatg gcggtgcagt
     9961 gctttccact acttttgata agtactgtta tgtgaatgtg cgtcatttgc ctagattttt
    10021 tgattacagc acagagttat catattctcg tactgagaga gtaacaaata ttgaagatat
    10081 cgagcatcct gcaattcgta atgcgatgaa aatgctagat atgcatgaaa ttcgccttac
    10141 atatgaagca gatcttcctg cacgttctgg tctgggtaca tctagttctt ttgccgtagg
    10201 tatgttgaat gctttctatg ctttgaaggg caagtacgct gacaagaaga aactcgttga
    10261 taaagcaatt tatcttgaaa gagtcctttg taatgaagca ggcggatggc aagatcagat
    10321 tgcagcagct tatggtggct ttaaccgaat caatttcaac tcagatggct atgaagtatt
    10381 gcctattatt attacgcctg agcgtaaaaa gcagcttaac aacaacttga tgatgttctt
    10441 tactggattc accagatttt cctctgatgt tcagaaggca aataatgtca gcggcacaga
    10501 ggacaaaaga gtaagactga aaaaaatgta tgagcttgtg gatgaagcag aagcggttct
    10561 gactgataag aacagagatc tagatgattt cggcagactg cttgatctca cttggagatt
    10621 gaaaaaggct accggtggtg cgattagcac tggaagtatc gatgaacttt atgaaaaggg
    10681 catggctgct ggtgcattgg gcggtaaact tctcggagca ggtggcggcg gtttcttggt
    10741 tttttatgtt cagcctgaaa aacaaaatgc tgtgcgttcg gcaatgagta atttgatgta
    10801 tattccgttt gaatttgaaa acggtggaac acaggttatt cattacacac cggaaatgta
    10861 tattccaaaa taaagaaatc agcgaggata agaatatgaa agttgttatt atggcaggcg
    10921 gtaagggaac tcgcatctca tcggttgcaa gcgatattcc aaagcccatg attaaaattg
    10981 aaaacaaacc ggtattggag cacgaaatcg aatgtctgag agaccaggga ttcacagata
    11041 ttattcttac cgttagccat cttggcgata tcattatgaa ctattttggc gatggttctg
    11101 gaatttcacc agttactggt cagccctttg acgttaaaat tgaatacttt ttggagaaag
    11161 agccattagg aaatgccggt gcattgtttc gtttaaagga taatttgacc gaagacttcc
    11221 tcctgctgaa tgcagatgcg atgtttgacg ttgatttcaa ccgatttgta gcatatcacc
    11281 gtgaaaaggg tggcttggtg acattgttta ctcaccctaa cagtcatcca tatgacagcg
    11341 gtctaatcat tgcagataag gatggctctg tagacagatg gcttgtaaag gaagattccc
    11401 gtccaactta ttaccgtaac agagtaaatg cgggactcca tgtgattagt cctgctgttc
    11461 ttgatgtcga gataaacaca cctaagattg acttggaccg tcagttatta aagccgttgg
    11521 caggaaccgg aaaaatgttc tgctatgata gtccagaata cgtaaaagac atgggtaccc
    11581 ctgatcgtta ttatgctgtc tgtgaagatt ttaaggctgg aagagtgaag ggcaaaaatc
    11641 ttcagaataa gcagaaagct gttttccttg atcgtgatgg aacgattaat caatatgttg
    11701 gctttctgcg tcatattgat gagttcgaac taatcaaggg tactgaagat gcagtgaaaa
    11761 agatcaatgc cgctggatat ctggctattg taataacgaa tcagccagta attgcaagag
    11821 gtgaagtttc ttttgaagaa ttggaagaga tccataacaa aatggagaca cttcttggat
    11881 ataagggcgc ttatttggat gcagtttatt attgcccgca tcatccacat aagggatatg
    11941 aggaagagcg cccagagcta aaattcgatt gtgaatgcag aaagccaaag cccggaatgt
    12001 taattcaggc agctacggac ttcaacatag atttgagtca gtcttggatg attggcgatg
    12061 gcgagaatga tattaaagcg ggccaagccg ccggctgtaa gactgcattg gttggcgaga
    12121 gtgagtatgg acaggatatg accatagcaa ccctgagtga atttgcagat aagatctgct
    12181 aagggatgtt ttatcacgaa aggagagtat aaacaatgat agacaataga ttacagaaac
    12241 acattgattt gctgattaaa cgctatccca ctcttgaagt aatcaagcag gatattgttg
    12301 acggatacct gattatggag gaatgctatt taaatgatgg caagctgctg atagcaggaa
    12361 atggcggatc tgctgctgat tccgagcata ttgcgggtga gctgatgaaa agctttaaga
    12421 cgcctcgccc tgttagtagg gaatttgcag ataaattgat tgcggttgat cctgtaaagg
    12481 gttctgaatt agctaagaat cttgaatatg gtttgatggc gattcctctc gttgctcatg
    12541 aagcgctttc cactgcgtat attaatgatg ttgatggcct aggtgttttc gcacagcagt
    12601 tgtttggatt tggaagacct ggcgatgtat tccttggtat ttcgacatcc ggcaatagca
    12661 aaaatgtgat aaatgcaact gttgttgcta gagctcttgg tatgaaagta attggcttaa
    12721 ctggagccaa aggtggagaa cttgcaaggg tagcagatgt ttgtgttaaa gctcctgaaa
    12781 cagaaacata tatgattcag gagtaccatt tgccaattta ccactgttgg tgtctaatgt
    12841 tggaagataa attttttggg aatagataaa atatctaagc ccatattgaa aggataaaat
    12901 tatgaatgta ctatttataa caactcaatt tccatatcct ctagataatg gcggaaaaat
    12961 tggtgcgtac aacggtatca gtgttttatc tagagactac gatgtgacgg tattgtcttt
    13021 caccgaagaa ccgcagtatg ttgaagaagg tgtcagtttt tttcgagaga aatttaatag
    13081 tgtaaaattt gaaaatcctg tgatacatga tgtacacata agaaataatc caatgaaatt
    13141 ggtagaagct ttagcaatag ggtattgtct acgccaacct tatatagttt caaagtttta
    13201 tgattcaaaa atgctgcagt taatagatga aaaattttct aacaatgcat attgggatat
    13261 catttttatt gactatctga atatgcaaag ttatggtcaa tacatacggg agaagtataa
    13321 aacacaattt ggtaagtata tcttaaaaga ccacaacata gaatatgatt tagtcctaca
    13381 ggaagctgaa tcaagtcgag gtttaaaaaa gaagttgctt cagttagagt ataagcgtac
    13441 tcgaaagtat gaagaggccg cagtaaatag ggccgattat tgctttagtg tgtgtgatgc
    13501 aaatacggaa gtactgaaaa agattaatga caacagttat acgatgcttc caacttatga
    13561 aatgcttaca ggggtgaaaa aaaatataga gaagaacacg atcctatatt tgggtaacct
    13621 ttcttggaaa tccaatatgc aaggattagt ttggttttta gagtcaattt atccaaatat
    13681 tcaacaaaga attccggatg taaaaatcac aattgtcggt agcgggccag ttggaacaga
    13741 ttttagtaaa tattcaggcg tggattatcg tgggtatgta aaagatattt cacacattta
    13801 tgacgatcaa gcggtgctaa ttgtaccttt gttagaaggt agtggtataa gaataaaaat
    13861 attagaagcg ttcaataatg aaattgcagt tgtatctaca ttagttgggt gtgatacgat
    13921 tggcgcaaaa gatggaagtg agttatttat agcagataac gaaaaagatt ttgcaacggc
    13981 tgtagttaga ctgcttgaag acatgaattt gaatgaaaaa gtaagaaaga atgcaaaatt
    14041 attcttaaaa gaaaaatact ccttaacggc tagacaagat gagtttagtg cgataattaa
    14101 cagctaatat atatataagc ttatctgatg atgatttata ggaggaaata tatggagatg
    14161 gctgagagaa aggaaaagaa acttggaaat aagaatatca tattatttta gaaaactcct
    14221 gttattttat gtttgtgtga ttgttgcggc tatgagttac caccttacag gcaattccaa
    14281 tgtagcgtca ttaattgctg tagtaggagt agtttatgct attgtaaagg cgtgtaataa
    14341 cgagtatctg gctatatggg ttatgtttgt attaggttct gatatatggg ggatagtgcc
    14401 atatattaac attggtagtg gtgtgttgca ttggtcagat tttaatgtta tagtagcgat
    14461 atttctctgc ttgagattca tcaaagttaa agctaggagt agatacggga ttgtctatag
    14521 tgtaatgctt atatttattt tgattgcatg tgtgcaatcg cattcaatct atggacaaga
    14581 tgtacataca acgctcttaa cagctagaga attgctagtt gtaatatcct tttggccgat
    14641 agtaaaaata gtatattcag aaagaataac taaaaatgaa ttcattgatt tagtgtgtaa
    14701 attcacaaga tggacaatgt gtatatattg gtttgaatgg attcttgtta atatgggatt
    14761 caatataacc tttttacaga caggtgcacg ctggggtact agaatctata tagatccgat
    14821 atttatcatt ttatatttct ttataaatct atatgcggtt atatataaaa gagagggcta
    14881 taatagtaca aataaaagta tatggaattg catttttagt ttactgacat ttgttataat
    14941 ctcgcaaggc agaagtggta tattatatgt agtaataacc tcggttatta tgcttttaat
    15001 ttcgcttaaa ccatccaaga ttattagatt tggtatatta ggaattgcct taattgcatg
    15061 tctagtgtct gtgacccaaa ttagagaggt cgttatatct tcgtttgtag agtctcagag
    15121 cagtgacaca ggtaatatcg catatagaat tgctgaacaa gattattttg aaaataaatt
    15181 agaaggtcat gaactatttg gagttggtat accaaacaat catgatgcca gagctgtaga
    15241 atattcaggc aaaacaatag attttgagtc taaaaaatat aatccctatt atcttgagga
    15301 cttaggcgct tttgaaattc gttatagatt tggtataatt gcatatgcgt tatatatagt
    15361 tgctttagct ataagtatta tagatgccgg atttcgaaga gacaaatcgg tcgccgcatt
    15421 tgcgggtatg gcattcatgg tgtttatggc gcttaatttt aatttgctag cgtatattac
    15481 agcaagaccg ttcggattta tgatttcttt aattttgata gaaatatcgg cattcaatga
    15541 tgaattcata tatattgaat aggtaaggga gagcacagat gaatttagat cgattaaaga
    15601 aaatagcaaa tatgatttca ccaatcataa aagtgataaa tgcgccgtta gcgtatttta
    15661 gtgggaaatc agattatatg gagaagggaa tattgtggcc tactgtaaca acctactcca
    15721 ataacagtag aaaagtgatt caatatacaa ctttacctaa aaacgtagac aacatggctc
    15781 aaatgcaatg ggctggggaa agttttgagg acatatgtat agttcttcaa ggtccagtat
    15841 tagaagacgg tgattttaca ctaaataccg ttaaaatgta taataaatac tatcctaaag
    15901 taaaacttgt agtgtctact tggataggat tatctgaaga gacagaatgc aagttgcgtg
    15961 aagctggtgc agatgttgta ttatgcaaga aacctgaaaa aacaggtgcg tacaatatta
    16021 atctacaact tacttctgca tacgaaggcg taaaaatggc gcaaaaactg gggtgtagtt
    16081 atgcgctgaa aacgcgaact gatcaaagac tatatgcaaa tgatgtacta aagtatttcc
    16141 aaacattatt gaatattttt ccatcttcgg actgtaaagt agtgaatcag cgattaatat
    16201 ttacgactta tggaaaatca tttagatatc ttccgtttaa tttatgtgat tttttggtat
    16261 acggagaaac tagtgaatta ttaaaattat atggtattca aagagataac cgagcatcag
    16321 actttgggaa aattatttat actgaggaaa aagagtttaa taaaaagata tttacactac
    16381 tagaaggaaa acaaggtgta tctccatatg atgtttttcc agattttgat gcactatata
    16441 aaaaatatat gtttgcagaa tactatattg tgtataatta tttcctggaa aatattgcaa
    16501 gtaatgaatc ttatggtgac ttaatggatg cctattatag ttatttaaag aaatatgcag
    16561 ttgttgctga ttcagaaaaa ttgttactat attggccgaa atatgttcaa tataatgtta
    16621 agttcgaaaa tgaacttgat ttctatgcga agttggattc aaaaaaatgg atggatattt
    16681 atgctaatta tacacctttt agtcagtgat tatatggaaa ggatgaggtg aataatgaaa
    16741 atcggaatag tgactattgt taatggggca agttttggta acagattaca aaattatgcg
    16801 actcaaaaag tgttggaaaa taacggctat acagttgaga caattaataa ttgttttgac
    16861 aaagaaagta ccctatccat tgttatcaaa aacgctattt atacattatt taagtttata
    16921 ccgcatttca aaaagtcaaa aagtatctgg aaaagaatgt atacatttta tcaatgggac
    16981 aagcagtata tagattattc tcatcgtaaa attgatgcat ctaaaaaaaa cagctttgtg
    17041 agtataaaga atgattatga ctatttcttg gttggtagcg atcaaatttg gaatccaaaa
    17101 tatgcagcca ataagggatt tgaactttta tcatttgttc caaatgaaaa aagagtttca
    17161 ttatcaacca gtttgggagt tccatctata ccaagtgatt tagaaaaatt atatgtgaca
    17221 gagtggaata agtttaaatc aatatcggta cgtgaaaatc aagcggcaaa aataattgag
    17281 gacttgacag gcacaactgc ggctgttcac attgacccaa ctttaatgtt ggagcgttcg
    17341 gaatgggaag cctttcaaaa aaaaccagac aatattgatg aaaattacat cttgtcgttt
    17401 ttcttaacta atccgcctaa ggatactgtt gatgtattag ataaggtcgc taaagatttg
    17461 aaaataggtg ttctcacctt agatgcgtct tgggaatatt ttcaagatat tactccgaat
    17521 cattttgttt atttgatttc acatgcaaga atgatttata cagattcatt ccactgcaca
    17581 gttttttcat tgctatttgg aaaaccggtt gtggtatttg accgttttga tagtgaaaaa
    17641 atgaattcta ggttaagtac attgttaaat aaagtaaata aagcacattt ttgggtagga
    17701 aaaaaagctg atgaagcaat gattcgtgca atagcatctg aacctgaata tgacatagat
    17761 aagctgatgg tatcagagag ggaatgtgta aaagcttatc ttaaagagta tttacagtaa
    17821 aattttgaaa tgggatgaaa atatggagca tttaagtgat tgttttggta aaatgaagat
    17881 tgttacgact tcatgcgcaa gagaatattt taaggatgat ttggataaat cagagtattc
    17941 atgttttgtt gattctcgag cagctatgtt tttcgcaatg ggacaagctt tagctaaaag
    18001 aaggaatgta gttgtaatgc ttgctgatga attgacatcg tgttatacgg cactaacaga
    18061 atgtgcattt caacaagttg atttaattgt gtttgctttt cagacagtca gacatgaaat
    18121 tgatttagca tctttgaaga attgtggttg caatctaatt aactgtagcg cccaagacga
    18181 tattgataga gttgtgtcca acactaaggg gttaaatcag acaacagtaa ttaaaatcaa
    18241 tatgaattgt gagtcggata aagatcatat atgtgagtat atagacctgc cagacatacc
    18301 tgtttttttc cacggtagct tatactcacc tgcaagtaaa aagaaatatg agcagtatgg
    18361 acaggtatcg aagtatttag gctatctcgt ggggggaaaa gatgcaatac tcataatttc
    18421 tcacaatgag tttttaaacg atatcagtgt atggattaat tattattctt ttggagaaat
    18481 tcaacccaaa attattatat ttgacagtcc gaatgactat caatataaat ggctgtgtga
    18541 cttagaatgc agtgtagctc attcaaccaa ccgatttttg tctgaggatt tagccttatt
    18601 gcaaaataac ggaatttcca ttgtgttaag aggtgataat tatgatatcg tcagttaaaa
    18661 atgttcaaat tttagtatct ttattaaaac agcataatat taaagatata gttatctcgc
    18721 caggtagtag agattttcaa ttggtgcata gtgtagaaac agacagtttt tttagaacct
    18781 atacggttgt agatgagcgc agtgctgcct tttttgctaa tggactttct atggcgattg
    18841 ataagcccgt gtgtgtatgt tgtacttctt caactgcctc ttcaaattat atggcggcag
    18901 ctaaagatgc ttttgctaat ggcatccaac ttgtacttct tactgctgat agagattacc
    18961 gccgcttata tcagatggaa gatcagatga ttgatcaggt agatatgtat ggggcttatg
    19021 tgcgttctaa cgttgatatt cctattgtta aggattctga tgatgaatgg tatgctacca
    19081 gaaaaataaa tgaagcaata ttggagttaa atcatggcgt taagggtccc gtgcagatta
    19141 attatcaatt aatggattta agtgcgccgt ttgttgagac catgccagag tatcgtaaaa
    19201 ttacaagata tcaagaggat gatttactgt catcagtaga gatttttaaa cagaaactat
    19261 tatcaaaaaa gagaataatg gttttatgtg gacagcagga agaaaataat ttaagtgcct
    19321 tattggaaga gttctttatt aaatataata caattataat caaggataac tattctaata
    19381 tacacggtga acactttatt aagactgttc tagtaacaga aagaatgagt gtaaatgcat
    19441 ttattgatta ttgcccagat ttggtaataa ctattggtaa acatgtatgg tcttttgtga
    19501 aatatactct tagagataaa aagcttaaat ttgagcattg gcggatatct gaggatggta
    19561 agattgttga tccgttcaac aagctaacat ctgtctttca gtgctcagcg gaatttttta
    19621 tggagactgt cactgctggt gtgaatagca ataataatct tgagtattat aatctatgga
    19681 agacaagatt atcaaaagtt tgctatccag agttagtcta tagcaatttt tatgcaattt
    19741 cagaactagt aaaaaaaatt ccagatagtg cattagttca ttctagtatt ttaaatgcgg
    19801 ctagattgta tgactttgta tctgatgata aagcattact cagttttgct aatttagggg
    19861 ctgatggtat tgatggttgt ttaccaacat ttttaggtca ggcagaagca gataaaactc
    19921 gacctgcgta tttaatttca ggagacttga gtttctttta tgctatgaat agtttatgtg
    19981 gtgacattcc agataatgca catattttcc ttattaacaa tcattctgga agtgaattcc
    20041 atacaaattt tggtaagttt tacaacggat cattaagtgt tgataagcat atagctgcag
    20101 gacatatttc aaacgcaaag gattgggcat tagaaaataa tataaaatac attactgcgg
    20161 attctaagga aagtctcaaa tcggggttgg atgtatttat taatacattt gacagtcctg
    20221 ttttatttga ggtctttaca gatccggata cggatacgtc tgttctggaa tctttttata
    20281 gtataaataa ggtaatcaca ccaaaaatgt attttagaaa ggcgttagat attataaaaa
    20341 agcatttaca cattaagtag atgtttattg cgaggtgaaa caaatatggc aataaaatct
    20401 tatattaaac ggttattgct ttatatccgt catggagatc ccaaaataaa tttagatgtg
    20461 agttatttat ctgaaggtgc aatgcttgct ggtaagactg tgataattac gggtggtgca
    20521 aaaggacttg gttttgcaat tgcaaaatca tttatagctc aaggcgcaag cgttattatt
    20581 acaggaagag acatggattc gttgaataat gctaaatgtc agcttggaga tatgtgttct
    20641 atcagggcgt ttgatataac tgatgctaaa atgtatcgaa cgttttttga agatgcatac
    20701 gagacctttg gacgtattga tatactgctt aataatgctg ggatctcgtt acacgaagat
    20761 ggatttatga gtgttactga gaaaacttgg gataagcaaa tggatacaaa tttgaaaggt
    20821 gtttatttct ttacacagga atttattaag tttgttaaag aaaagaatca aatgcaagca
    20881 aatattatta tgatgtcctc agaaagaggc ttgtgtggtg atagtattcc gtatggatta
    20941 tcaaaggctg caattaactc tttagttgta ggcttgtcta agaaatatat ttctgaggga
    21001 attagggtta atggtattgc tcctggagta actgcaaccg ctatgactgg tatttctgaa
    21061 aagggtaatt tgtataatcc tgcaacgatc ggttatagat attttctccc agaagaagtt
    21121 gctcaagttg ctttgttttt ggcaagtagt ctttcaaact gtatcagtgg tgaaatcata
    21181 gcctgtgacc aggggcaata tttaagtcga aattgggggt aataacttgg gaatattatt
    21241 aaaaaaatat aatagcctta gtgagcctgc aaaagcatct atttggttta cactttgtaa
    21301 tgtgttgcaa aaaggaattc tatttttgac tacacctatt tttacaagaa tgctttcaac
    21361 tgcagaatat ggtaagtata cgatttttat atcgtggtat tctattatag caattttcac
    21421 aacgttgaat ttatctgcag gtgtctataa taaaggacta attaaatacg agaataaata
    21481 tgcattaacc tcgtcatttt taggattatc gtcatttatt tccctttgtg cattgatgct
    21541 ataccttcca tttagaacgg ttataaatgg gctactggat ttatcgacgg agtttgtcat
    21601 attgattctt attgaatcaa tatttgagcc ggcttttttg ctttggtcag ttaaggaacg
    21661 atttgattac aagtatcaaa aactagtact catcacagtt cttatggctg ttttaaatcc
    21721 aggtctcggt attatattaa tcttatcatt tggggagtat gctatttgga gagtacgagc
    21781 ttatgtcgtg gtaaatgttg tagtggggat gtttattggc ataggaatac tggctaaggg
    21841 taagaaatta tttgataaag agtactggac atatgcgctc agatttaatt tgccattaat
    21901 accacattat ttatcaatga cattgctgaa tcaggttgat cgccttatga taaatagtat
    21961 ggttggagct agcgaggctg cgatttatag tatcgcttac actatatcta gtatgatgaa
    22021 tatcataaca acagctataa acggttcttt tattcctttt acatacaaag caattaaaga
    22081 tggcgtgtat gaaaaaatta aatccgttgc taatcaacta ttagtgttga tttttgtctt
    22141 gtgttcgttt actatgctgt tcgggccaga attgatttgg gttgtcggag gagaaaaata
    22201 tatggatgct gtttggatta ttcctccagt tgcggcatca gtatttttca tgtttttata
    22261 tccgcttttt ggcaatattg aattttacta tgaaaagaca aactacgtaa tgtttgcatc
    22321 ttgtgtgggc tctatcgcaa aaattatttc aaactatgtt agtattcccc gttttggata
    22381 tatttcggct ggttacacga cattgatttg ctatatgttg ttttcctttg cccactacat
    22441 gttttataaa aaaattctta gagaaaatgg tataaaagaa gatatgtata atattaaaaa
    22501 tattatattt ctttctgtct taatgattgt gttaactatt ggtatgacaa tattatactt
    22561 ttctacaatt ctaagatata gtattattat tggattagtt attgtggctt ttcttaaaag
    22621 gacgcaaata atgaaaatcc ttaaaaatat aaaaaattaa caagcaaatc ttgggaatgg
    22681 aacggaggca cggtgaaatg agattattga ttccatctgc taagattgta ccggaagaac
    22741 ttcaccatct tggaaaactt cctgcaatta tctatccgat taatcaaaaa attgtatttg
    22801 att
//
LOCUS       DACEND010000036        22564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEND010000036 DACEND010000000
VERSION     DACEND010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.037"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..753)
                     /locus_tag="IW746_001145"
     CDS             complement(34..753)
                     /locus_tag="IW746_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1078057.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            1090..1422
                     /locus_tag="IW746_001146"
     CDS             1090..1422
                     /locus_tag="IW746_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078058.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1409..3412
                     /locus_tag="IW746_001147"
     CDS             1409..3412
                     /locus_tag="IW746_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1078059.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3431..3901
                     /locus_tag="IW746_001148"
     CDS             3431..3901
                     /locus_tag="IW746_001148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1078060.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3922..4506
                     /locus_tag="IW746_001149"
     CDS             3922..4506
                     /locus_tag="IW746_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1078061.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4521..5507
                     /locus_tag="IW746_001150"
     CDS             4521..5507
                     /locus_tag="IW746_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1078062.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            5497..5808
                     /locus_tag="IW746_001151"
     CDS             5497..5808
                     /locus_tag="IW746_001151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1078063.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5830..7611
                     /locus_tag="IW746_001152"
     CDS             5830..7611
                     /locus_tag="IW746_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1078064.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7604..8980
                     /locus_tag="IW746_001153"
     CDS             7604..8980
                     /locus_tag="IW746_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1078065.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8987..9622
                     /locus_tag="IW746_001154"
     CDS             8987..9622
                     /locus_tag="IW746_001154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1078066.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9652..11007
                     /locus_tag="IW746_001155"
     CDS             9652..11007
                     /locus_tag="IW746_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1078067.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKFAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11199..12593)
                     /locus_tag="IW746_001156"
     CDS             complement(11199..12593)
                     /locus_tag="IW746_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1078068.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12685..13656
                     /locus_tag="IW746_001157"
     CDS             12685..13656
                     /locus_tag="IW746_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1078069.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13653..14434
                     /locus_tag="IW746_001158"
                     /pseudo
     CDS             13653..14434
                     /locus_tag="IW746_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(14603..14974)
                     /locus_tag="IW746_001159"
     CDS             complement(14603..14974)
                     /locus_tag="IW746_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1078070.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            15108..15551
                     /locus_tag="IW746_001160"
     CDS             15108..15551
                     /locus_tag="IW746_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1078071.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW746_001161"
     CDS             complement(15594..16403)
                     /gene="yidA"
                     /locus_tag="IW746_001161"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1078072.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16422..17789)
                     /locus_tag="IW746_001162"
     CDS             complement(16422..17789)
                     /locus_tag="IW746_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1078073.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17773..18603)
                     /locus_tag="IW746_001163"
     CDS             complement(17773..18603)
                     /locus_tag="IW746_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1078074.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18766..19203
                     /locus_tag="IW746_001164"
     CDS             18766..19203
                     /locus_tag="IW746_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1078075.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19279..19899
                     /locus_tag="IW746_001165"
     CDS             19279..19899
                     /locus_tag="IW746_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1078076.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20088..20594
                     /locus_tag="IW746_001166"
     CDS             20088..20594
                     /locus_tag="IW746_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1078077.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20611..21510
                     /locus_tag="IW746_001167"
     CDS             20611..21510
                     /locus_tag="IW746_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1078078.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21597..22482
                     /locus_tag="IW746_001168"
                     /pseudo
     CDS             21597..22482
                     /locus_tag="IW746_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT         7308 a         3923 c         4847 g         6486 t
ORIGIN      
        1 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
       61 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
      121 gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc
      181 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
      241 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
      301 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
      361 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
      421 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
      481 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
      541 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
      601 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
      661 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
      721 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
      781 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
      841 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
      901 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
      961 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
     1021 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
     1081 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
     1141 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
     1201 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
     1261 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
     1321 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
     1381 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
     1441 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
     1501 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
     1561 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
     1621 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
     1681 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
     1741 catgaataaa ttagaagagg tccttgattt aaaagaaaag tgggaacagc taactgaaaa
     1801 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
     1861 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
     1921 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
     1981 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
     2041 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
     2101 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
     2161 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
     2221 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
     2281 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
     2341 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
     2401 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
     2461 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
     2521 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
     2581 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
     2641 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
     2701 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
     2761 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
     2821 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
     2881 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
     2941 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
     3001 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
     3061 tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
     3121 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
     3181 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
     3241 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
     3301 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
     3361 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
     3421 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
     3481 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
     3541 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
     3601 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
     3661 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
     3721 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
     3781 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
     3841 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
     3901 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
     3961 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
     4021 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
     4081 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
     4141 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
     4201 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
     4261 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
     4321 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
     4381 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
     4441 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
     4501 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
     4561 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
     4621 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
     4681 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
     4741 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
     4801 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
     4861 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
     4921 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
     4981 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
     5041 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
     5101 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
     5161 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
     5221 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
     5281 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
     5341 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
     5401 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
     5461 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
     5521 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
     5581 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
     5641 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
     5701 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
     5761 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
     5821 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
     5881 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
     5941 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
     6001 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
     6061 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
     6121 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
     6181 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
     6241 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaaggt
     6301 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
     6361 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
     6421 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
     6481 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
     6541 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
     6601 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
     6661 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
     6721 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
     6781 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
     6841 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
     6901 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
     6961 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat cgccgtctgg aggggatatt
     7021 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
     7081 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
     7141 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
     7201 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
     7261 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
     7321 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
     7381 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
     7441 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
     7501 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
     7561 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
     7621 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
     7681 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
     7741 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
     7801 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
     7861 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
     7921 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
     7981 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
     8041 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
     8101 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
     8161 cacttttgaa gaagcagaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
     8221 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gagcgtatcg ctactcctcg
     8281 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
     8341 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
     8401 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
     8461 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
     8521 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
     8581 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
     8641 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
     8701 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattggc
     8761 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
     8821 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
     8881 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
     8941 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
     9001 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
     9061 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
     9121 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
     9181 ttttgtattg gcaaatgctt cgatcacgga gcctttcatc gaagaattat ttgttttgcc
     9241 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
     9301 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
     9361 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
     9421 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
     9481 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
     9541 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
     9601 aaatatgggt accactgact aagtggtatc cttttttaaa agaggaaaac gatgattaaa
     9661 caaagcaaac aaataaagaa acgcttgccg gctgttcagc ttctcgcatt aggatttttt
     9721 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
     9781 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt gacgggattg
     9841 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
     9901 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
     9961 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    10021 tcaggtgtga tgaagctcat gctttacatc ctgaaatttg cctttgtgat ccaactgatt
    10081 ggtacggttg ctttatcttt tgtattcgtg cctgaatttg gctggttgaa aggtatctgg
    10141 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    10201 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    10261 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    10321 ataacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    10381 gttgtgttct ttttcacaga gcgcaatggg gaaacgctag tggaagggaa tatgtttcaa
    10441 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    10501 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggt
    10561 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    10621 cagatgcgtt cgatgcttaa aggacgtacg agagcggaag catttggccg taccatccga
    10681 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    10741 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    10801 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    10861 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    10921 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    10981 ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt
    11041 aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg
    11101 tttatttttt gaggcactat ccgttgttga acctcgggta gtgcctcttt cttattgaaa
    11161 ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata tttttttatt
    11221 ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa
    11281 attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg
    11341 ataccgttag caagaaagtc attctcaatt atcttaggat caatctcagg atcaactgga
    11401 agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa
    11461 agctgattgg ctcttttcgt aagacttaat ttagtttcta agatcctatt tgcttcattt
    11521 gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta
    11581 aaaattgctt gttctatctt gttttggaca tgtactggaa ctacaagata agcaactcgc
    11641 aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataat
    11701 ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca
    11761 tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg
    11821 tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc
    11881 ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca
    11941 attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat
    12001 cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca
    12061 ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt
    12121 aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc
    12181 aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta
    12241 cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta
    12301 gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta
    12361 tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa
    12421 tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc
    12481 tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt
    12541 tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctac
    12601 actccttaaa attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa
    12661 tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct
    12721 tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt
    12781 aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaaga caaaaatatg
    12841 tttgcgatga tgcctgctta tttgggggag aacagatatt ttggaacaaa agtcattacc
    12901 gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt
    12961 gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga
    13021 acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt
    13081 gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt
    13141 cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct
    13201 aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca
    13261 gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa
    13321 gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga
    13381 gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctacc
    13441 ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt
    13501 attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg
    13561 aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat
    13621 atttataaca aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg
    13681 catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc
    13741 tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta
    13801 cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc
    13861 tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg
    13921 atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt
    13981 attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg actgaatatg
    14041 aagaagcaat tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat
    14101 tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg
    14161 aattaggaat agagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac
    14221 cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct
    14281 tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc
    14341 aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc
    14401 agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa
    14461 ttggtggaga gtggcagcct tatagctaca atggttgtta aaaaaagacg caaacttttt
    14521 caatttaatg aaaagtttgc gtcttttttt aattattggt gtttgttttt tattcaatca
    14581 agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt
    14641 caatgacaaa gtagtaagaa ggcgtagtgc caggtaatcc ttttaagtca gtaatctcaa
    14701 gacctgcagc tttatgttgc tcgtgtaatt tttctaaatc atcagctgca atagcgatat
    14761 gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt
    14821 catagtcatc tccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac
    14881 ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat
    14941 ctttcacccg aacacatgtg tgagccattt tcataaattc atctctcctt ttccttttac
    15001 tcaatcataa caaaaagaag taagaaaata gttgatttgt gcttgttcat gagaaatttc
    15061 tcatgtaaaa tggatagaaa ataatactgt taaggtggaa acaattcatg gataaaccaa
    15121 tttttggtga aaaaaagcaa tctgctaatt atcaaacccg ttatgcagca tatattgtca
    15181 tagaaagaga caagcggatt gcggtgatcg aggctccaaa tggtgctttt ttccttcctg
    15241 gtggggaaat cgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag
    15301 aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact
    15361 cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga
    15421 agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag
    15481 cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa
    15541 ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt
    15601 acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt
    15661 gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca
    15721 atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca
    15781 aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg
    15841 aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc
    15901 gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg
    15961 tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg
    16021 tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa
    16081 tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgagtc
    16141 agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa
    16201 gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct
    16261 ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc
    16321 gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg
    16381 atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat
    16441 gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga
    16501 tcacgcaagg agggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct
    16561 tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca
    16621 tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa
    16681 ggcagatcgt agctagaatt cacagcagtg taataaacag ggttatagcc aaccttttct
    16741 actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat
    16801 ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat
    16861 tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct
    16921 aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg
    16981 gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat cacttccata
    17041 ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa
    17101 tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc
    17161 acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc
    17221 cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt
    17281 gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact
    17341 cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct
    17401 tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca
    17461 tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct
    17521 attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa
    17581 acgcctaatg aatgggtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg
    17641 cctaattgtt taatgcgtcg taaacgttgg acttctttcg aattgatcaa atctaagatt
    17701 actttatgct ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc
    17761 agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc
    17821 cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt
    17881 agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt
    17941 gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg
    18001 atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg
    18061 cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga
    18121 aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa
    18181 atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg
    18241 aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt
    18301 tgtgtcactt tgagggaaaa tcaaagaaat atttaaaatt ccttcatctg atacgatccc
    18361 taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc
    18421 gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg
    18481 ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa
    18541 tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt
    18601 cattcaaccg gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa
    18661 agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa
    18721 gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa
    18781 ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat
    18841 ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat
    18901 aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat
    18961 ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa
    19021 gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg
    19081 aaagaactgc atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat
    19141 gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg
    19201 taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg
    19261 tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta
    19321 tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct
    19381 gatttagcta accagatcca aaactatctt gagaaatcag acagtgaaat tcgtgattca
    19441 ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat
    19501 cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga
    19561 atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt
    19621 atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg
    19681 acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct
    19741 gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac
    19801 ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat
    19861 tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg
    19921 ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc
    19981 ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt
    20041 tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg
    20101 gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa
    20161 ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct
    20221 tagatgtgat gcaagatagt tttcataaag ccttgaacta ttttgaaaaa gaaaaagaga
    20281 tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc
    20341 gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac
    20401 ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa
    20461 aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcaactatct
    20521 tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc
    20581 aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa
    20641 ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag
    20701 cgttttatta tcgaagagca atcatttata aaagcaaggc ggagaaagag acgtctccaa
    20761 gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt
    20821 caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta
    20881 ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca
    20941 caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga
    21001 caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa
    21061 gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag
    21121 atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa
    21181 aacagtgtcc agctctcact tccactttta ttcaaagatg acacttatct atctgttttg
    21241 acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat
    21301 tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca
    21361 cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt
    21421 gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt
    21481 ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa
    21541 ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga
    21601 aaagaaatta tttagtcatc agtattattg ctttatccgt gcttttgttt ttcgaaatta
    21661 cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt
    21721 cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta
    21781 acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcaatca tcacaatcaa
    21841 cacaagacag tacagaagca atccaagacc cgactgtcaa aagcgtcgca atcagctttg
    21901 atgatggacc aggagcgacc acaacgccgc agcttctgcg aatcttaaaa gaaaaaaacg
    21961 tgcatgtcac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc
    22021 agacagctga agcagggcac gagattggca atcacacata cgatcatcag aatctggcca
    22081 ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaaa
    22141 gtcactggca aaacccccgc ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
    22201 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
    22261 aggaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg gtcaattatt
    22321 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
    22381 aaagagcaag gctatcgcat cacgacagtt agcgatttat tagggcatcc aacggctgtc
    22441 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
    22501 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
    22561 caat
//
LOCUS       DACEND010000037        22457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEND010000037 DACEND010000000
VERSION     DACEND010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.038"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..152
                     /locus_tag="IW746_001169"
     CDS             <1..152
                     /locus_tag="IW746_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1078079.1"
                     /translation="LLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAERVSVNEME
                     ENQID"
     gene            145..846
                     /locus_tag="IW746_001170"
     CDS             145..846
                     /locus_tag="IW746_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1078080.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            846..2432
                     /locus_tag="IW746_001171"
     CDS             846..2432
                     /locus_tag="IW746_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1078081.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2451..2672
                     /locus_tag="IW746_001172"
     CDS             2451..2672
                     /locus_tag="IW746_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1078082.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2669..3430
                     /locus_tag="IW746_001173"
     CDS             2669..3430
                     /locus_tag="IW746_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1078083.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3810..4319
                     /locus_tag="IW746_001174"
     CDS             3810..4319
                     /locus_tag="IW746_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1078084.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4394..4933)
                     /locus_tag="IW746_001175"
     CDS             complement(4394..4933)
                     /locus_tag="IW746_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1078085.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5073..5684)
                     /gene="rpsD"
                     /locus_tag="IW746_001176"
     CDS             complement(5073..5684)
                     /gene="rpsD"
                     /locus_tag="IW746_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1078086.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6000..6797
                     /locus_tag="IW746_001177"
     CDS             6000..6797
                     /locus_tag="IW746_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1078087.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6825..7460
                     /locus_tag="IW746_001178"
     CDS             6825..7460
                     /locus_tag="IW746_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1078088.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7480..8253
                     /locus_tag="IW746_001179"
     CDS             7480..8253
                     /locus_tag="IW746_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1078089.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8661..9458
                     /locus_tag="IW746_001180"
     CDS             8661..9458
                     /locus_tag="IW746_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1078090.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9436..9849
                     /locus_tag="IW746_001181"
     CDS             9436..9849
                     /locus_tag="IW746_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078091.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9833..12478
                     /gene="mutS"
                     /locus_tag="IW746_001182"
     CDS             9833..12478
                     /gene="mutS"
                     /locus_tag="IW746_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1078092.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12496..14604
                     /gene="mutL"
                     /locus_tag="IW746_001183"
     CDS             12496..14604
                     /gene="mutL"
                     /locus_tag="IW746_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1078093.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14626..15186
                     /locus_tag="IW746_001184"
     CDS             14626..15186
                     /locus_tag="IW746_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1078094.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15271..17259)
                     /gene="treP"
                     /locus_tag="IW746_001185"
     CDS             complement(15271..17259)
                     /gene="treP"
                     /locus_tag="IW746_001185"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1078095.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17482..18195
                     /gene="treR"
                     /locus_tag="IW746_001186"
     CDS             17482..18195
                     /gene="treR"
                     /locus_tag="IW746_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1078096.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18272..18718
                     /gene="msrB"
                     /locus_tag="IW746_001187"
     CDS             18272..18718
                     /gene="msrB"
                     /locus_tag="IW746_001187"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1078097.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18888..19490
                     /gene="ruvA"
                     /locus_tag="IW746_001188"
     CDS             18888..19490
                     /gene="ruvA"
                     /locus_tag="IW746_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1078098.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19503..20504
                     /gene="ruvB"
                     /locus_tag="IW746_001189"
     CDS             19503..20504
                     /gene="ruvB"
                     /locus_tag="IW746_001189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1078099.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20533..21111
                     /locus_tag="IW746_001190"
     CDS             20533..21111
                     /locus_tag="IW746_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1078100.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21163..21645
                     /locus_tag="IW746_001191"
     CDS             21163..21645
                     /locus_tag="IW746_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1078101.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRGAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21762..22136
                     /locus_tag="IW746_001192"
     CDS             21762..22136
                     /locus_tag="IW746_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078102.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7326 a         4037 c         4867 g         6227 t
ORIGIN      
        1 ttttactggg agcagtcgtc gtgtgttcca tcgtcacgat ggttgcagcg ggaaaaacgg
       61 cagaaggtgt agaagctttg ctgggctatg tccgtaagaa aaaagcagaa agggtatctg
      121 taaacgaaat ggaggagaat caaattgact gagttttttg tgaatccatt atttggctta
      181 agtttgactt taggagtgta cgtattatct gacaaatttt tgcggaaata taaactgcct
      241 gttttgaatc ctctcgtatt atcgattgtt gttattatct tatttttgca tttttctggg
      301 atcagctata aagactataa tatcgggggc aattttttga ctatgctgat tactcctgcg
      361 acagtggcac ttgccatccc tctttataaa aacttccgtt tactgaaaga gaatttttat
      421 cctgtaatgg ctgcgatttt agcaggaata gtcgcaaatt gcttggttag tgtagggttc
      481 ggttacttct tctctcttca taaagagatg gtgatctcat tactacctaa atctgtaacg
      541 acggctattt ctgtcgattt atctcatacg atgggcgggg tgaatgccgt gacacttgct
      601 attgttgtct ctacaggtat ttttggttcg ctgatcgcca ctcatatttt ccgcctattc
      661 aagatcgaaa gtccagttgc tcgcggtgtg gcgttaggaa gtacttctca tgctattggt
      721 acagcaaaag caatcgagat tggtgaaata gaaggaatca tttccggact tgcgatatgc
      781 gtcaacggaa tattgaccgt tttattgcta ccgttattct ttcagccttt tgctggcttg
      841 ttttgatgcg aaaaccacta ttttatgcta caataaccaa aacgttcgag cataaaaaag
      901 gagggaaaga cgtgtatttg tcttccgagt atcttaaaca atttgataaa gatctatatt
      961 ataagattct cctcctagaa agttttgagg atcaagcttg gcataccgct gcacaattag
     1021 cgcaagttgt tcagctggat gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa
     1081 actatcagca gtttagcggt aagacacacc ctttgtttac aaaaaatcac cgttctggtt
     1141 ataactttta tgatacgctc gattcaattg aacatgagcg ttttttgatc tatctcgtgc
     1201 aaagtacgct gaaattccag ttgctgcacg acattttttt tgaagaattc catacaatgt
     1261 accaatttgc gcaaaagcat tacattagtg aatctactgc tcatcgaaaa atcaatgaat
     1321 ggaaacagca actgcaaact tacggaattc ggctgcaacg aggtacatat atcgctcaag
     1381 gcgaagaaga aatcattcga ctttacttgc atatgacatt ctggcagttg tttcgaggga
     1441 aaatctggcc atttgaaacg atttctcaaa tggacgtaaa gaatatggca gaacatatca
     1501 tggctttttt taatgttcgt ctgaacgaga tcaaaaaaag acgattggaa tatatgctag
     1561 gtgccttttt tttaagaaaa tcacagaagc attatgttgt attgaatgag aaaaaaagac
     1621 ggctgatttc tgacaatctg ttgttccagc gattttgcca ggtcatggag ccggtatttc
     1681 ctaattattt tcaagtagaa gacgagttag gcgcattatt tcttgtactg atgactcgtg
     1741 aagaatatta tagtgatcct aagatacgga agaaaatttt tgattttcat caacaagcaa
     1801 aaacaccgcc ctttacagcc ctttcagaag caaaagccgc tctttctctt tatcaggaag
     1861 agcaggggct gccagcagaa aatctaacct tcgaggcaga aaactattta ttcagcagtc
     1921 attttttcgc ctatcttttt cctaatgcga aagaaacgat tgatggcaat agtagtgatt
     1981 ttattaatca tttagttatt gaaaacaagg aattgaaaca atggctcgta catttttttg
     2041 aaagtagaca caaacatccc aatcatctgg catttaaaaa tcatgcgttt ttgatggggc
     2101 ggtatctgac tgtgtttaaa acattaggag cctttactcc acagcttcca aagatcacga
     2161 tccttttaat gacagatttc ccattatttg aagagcaact gttagaggag ggattacgga
     2221 catttttccg taatgagtat cagctgattt ttctgccaac agattatcgg gggagagaag
     2281 ttgatcttct gatttcgaca agtaaagtcc accgcaaacc atgggcggat cttgactatt
     2341 ttattgtgac tgaagaacta aaattaattg attacatcca actatctcaa aaatttgaaa
     2401 tgattcaaaa acaaaaacta ttaaaacaat aacaaagaag gagaaaaaag atgacatttg
     2461 aagaaatttt accagaattg aaaaaaggtg caaagattat tcgtaaagga tggagtggat
     2521 ttgaacttta tgttgttctc gtatccgatg atgaatttga tggctgtccg gtgacgccgt
     2581 acttattgat caaaacaagt gacgaaggtt tttccagctt cgctcctaca gtttgtgata
     2641 ttttagcaga tgattgggcg atcgtagaat gaaaaaccag tatccagatc tagctaaaca
     2701 ggttgtattt atcacaggag ctgcttctgg aattggtgct gcgcaagccc gagcgtttct
     2761 tgaacaagat gcttttgtat ttggattaga tcaaaagttc ggtaaaatgg aagaaatttc
     2821 tttgcgattt cctgaaactt ttgcctacgc actaggtgat gttcgacaaa tgaaagaact
     2881 ccaacaagcg gtatcgaaat gccaaaatca ttttggtgaa gttacgattt tactgaatac
     2941 agcggggata ttggatgcct acaaaccctt gatggaaact gacgaatcgc tgtgggatct
     3001 gatttatgag acgaatgtga aaagcatgta tcagctgaca aaaatcatcc ttcctgatat
     3061 gatccgccaa aaaagcggaa cgatcatcaa tatggcttcg attgctggct tgattgctgg
     3121 tggaggtggg atcgcttata caagcgcgaa acatgcgatt gtcggattta ccaaacaact
     3181 cgctctggat tacgcaggac agggtatccg agtaaaagga attgctccag gagccatcca
     3241 aacaccaatg aatgcggctg attttgaagg gaaaggtgag atggcagagt gggtggcaaa
     3301 tgaaacacca gtcaagcgat gggcacagcc ggaagaagta gctgagctga cgttatttct
     3361 tgcctctcct caagcaagct acatacaagg aacgatcatc ccaatcgacg gcggatggtt
     3421 gttaaaataa ccttgtgttt ttttcaagct tctgtatgat aaaactaaag agagaaacaa
     3481 cggttgatga taaaaaaagt cgatgactgt tcccgacatt cgatgccgaa cagccagttc
     3541 aaaaaattct ttttaataat cgttaatatt cgaaactaag cggcttttca cgacctcttg
     3601 atataaacga tattcaaaag cttgcaactg cggtaacaag tccagccaaa tgaaaaatat
     3661 aaggagctat ttttatttga ccattcttat tacttgttgc agggcagctt tttcactacc
     3721 ttctgcgaca cttcagttgg aattttcttc ccatcctttg gtcgcggtca ttaccaagat
     3781 aaaattatct tgcaaaggag aaatagaaca tggaaaaaaa taatacccgt tcagccaaac
     3841 gctggacaac gacagatgtg actcggatgg cgctagtcac tggtttatat gtggcagtga
     3901 cgctggtttt gtctgtcttt agctttggag tgattcagat tcgtctctcg gagatgttca
     3961 actatctgtc cttgtataac aaaagataca tcgtcgcggt aacagcggga gtagctttgg
     4021 ctaatattgc ttctcctctt ggactgatag atgttgcagt aggaagcgtc agcacgctgc
     4081 ttgtattgct tatcaactat aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg
     4141 ccgtgacagc atgcgtattt gctttttcca tgtttacagt agctggtcag ctaacgattt
     4201 tatatcaact accttttttc ttgaattggt gggtgattgc tttaggagaa ttggcatcaa
     4261 tgattgtcgg tgggataatt atttattgga tcggtaaaaa agtggatttg tcaaaataaa
     4321 gatctgttta atttaaaata gggcagcatg gaagagactg aatgttgagt cttcttccat
     4381 gctgcttttt tgattaattt gtatgaagaa atcgatttag ccgttttttc atcaagatac
     4441 cgatataagc tgctccagtt gccttgattg cttccggcag taaaaacggc agtagtccac
     4501 tggcaaatgc agttgtaaaa gtaagatcac cacttatttt cagccagatc gttccgcaga
     4561 tcaatgcagc aagagagccc attagattgg caaagattgc atataagtaa tgagcatccg
     4621 ttctttcgat catccaacca gttatccacg tttgtaagag aaatccaatc aaatatccgc
     4681 cagttggccc aaataaaaca gctatacctc cagtcatccc tgaaaataca ggaagaccaa
     4741 tcaagccaag taaaagataa actccaacag atatcaatcc tagttttctt ccgagaaaag
     4801 ttgctgttaa tccaatagca aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg
     4861 tcatctgaga aaggacagaa ataatcgcgg caaattccgc agcaagtatc aattctttca
     4921 atgttcggtt cataacgccc tcttttcgtt aacttaatat ttttatttgg ttaacgaaat
     4981 tgtaaaacaa agtgattact ttgtaaatag aaaatataaa aaaagaccag ctatccaata
     5041 gatagcggtc tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg
     5101 acaagagctt cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa
     5161 ccttccaatt tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca
     5221 gcttctttta ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact
     5281 tctacgtggt atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta
     5341 actaattgac gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct
     5401 aaacgttgtt ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta
     5461 gcttttacga acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt
     5521 tgtttttcag ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt
     5581 tgacctggtg cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct
     5641 aagcgacgag aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat
     5701 gaatataagt ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc
     5761 ttcaatcttc acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact
     5821 agggatgagt tattattttt ctgctgcatt attttacaca aactacagta tatgatagat
     5881 catccacgaa tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt
     5941 ataaaatgag agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa
     6001 tgaatccggt atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc
     6061 tttttgaaaa cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga
     6121 ctttaggaac ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc
     6181 taggaaagat tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga
     6241 acgccgaatt aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac
     6301 ttgctttaag taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg
     6361 gtatattatg tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca
     6421 actttatgtt agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg
     6481 aagggatgtt tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag
     6541 aggatgtcgg aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat
     6601 atgaacacgt gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac
     6661 agatggatgg aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa
     6721 attatattgg tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat
     6781 catcctatgt ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt
     6841 ttccaaaaaa tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct
     6901 ttgctggagt acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca
     6961 aaaatttatc tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa
     7021 gagagtatca gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg
     7081 aagacttagt cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact
     7141 cttggagaaa agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct
     7201 ttcaattgtt tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat
     7261 taggttataa acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc
     7321 aaacaaagga attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga
     7381 ccgatgccga ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag
     7441 aggcaagtaa gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct
     7501 tttgatattg atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa
     7561 gcattggaaa aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga
     7621 tttcatgcgc aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga
     7681 gcggccggtt ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg
     7741 gatgcgttcg ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac
     7801 aatatcaagc gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc
     7861 ggtgctgtct tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg
     7921 ttagcatttt tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt
     7981 gtccgctggc atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca
     8041 attctgcact tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat
     8101 ggacaaaacg accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct
     8161 gtgccagaag tacaagcaga agctaagatg gtcactgata caaacgatca ggatggaatt
     8221 tggaaggcat tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca
     8281 tcaagtaata agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt
     8341 attaccgcag atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc
     8401 agtttcaaga aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg
     8461 gcttatttct gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga
     8521 tgtttgtcat agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga
     8581 gttccttttg tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta
     8641 aaagagaaga ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg
     8701 gacgagagat gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa
     8761 cgattgtaaa tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa
     8821 aatttttgca agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg
     8881 gaatctttga gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag
     8941 ggacgcctgg acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca
     9001 acatgcaggc tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc
     9061 tggtagaaga agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa
     9121 caagtgaaaa acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta
     9181 cacatacaca cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga
     9241 cagacgttgg gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa
     9301 tcgaaaagtt tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga
     9361 ttttaagcgg ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc
     9421 tgattcagat caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag
     9481 caggagccgg aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa
     9541 acgatcattc gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag
     9601 ttgagagaaa aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa
     9661 ccagcagcag aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag
     9721 catccgcttg ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat
     9781 ttaacaacta tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca
     9841 aaaaactaaa aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga
     9901 tgcttttttg ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa
     9961 agcgtcacag cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat
    10021 tcctatgtgc ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa
    10081 aggctataaa gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt
    10141 caagcgcgaa gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc
    10201 ggcaaaagac aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc
    10261 ttatgccgat ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt
    10321 tttaaatgaa gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga
    10381 tacccttcgt gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat
    10441 ggaagaaaac gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga
    10501 agcaaccggc aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat
    10561 ccaaaaagca gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt
    10621 caacctagaa ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt
    10681 gttagatgaa acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc
    10741 gcttatccag gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag
    10801 ctattttgaa cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct
    10861 agcaggtcga gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc
    10921 tttagaacaa gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca
    10981 aagtctgctt tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat
    11041 ccaagatgat ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga
    11101 gcagctcgat atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga
    11161 agcaaaagag cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt
    11221 cggttattat atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga
    11281 acgaaaacaa actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga
    11341 gacacaaatc ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc
    11401 tgtgagagaa gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag
    11461 tgcagtagat gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc
    11521 agaacttgtc agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga
    11581 aaaagtatta ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat
    11641 gatcttgctg attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc
    11701 attaactgtt ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc
    11761 tatttttgat cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag
    11821 tacttttatg gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag
    11881 tttgattttg tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc
    11941 ccaagcaatc atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca
    12001 ttatcatgaa ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg
    12061 agccgttgaa aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga
    12121 taaaagctac ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg
    12181 agcagcagat atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc
    12241 tgaagtagaa gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa
    12301 agtaaatcaa gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt
    12361 cagtgaatta tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact
    12421 agaaatgacg ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga
    12481 agaaggcgga tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc
    12541 gctgcgggag aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca
    12601 attgacgccg gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc
    12661 caagtgattg ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg
    12721 catgcgacaa gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt
    12781 cgaggggaag ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat
    12841 caggaagaaa aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga
    12901 ccagcaccac tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca
    12961 gcccgcttga aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc
    13021 aatcgactgg cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa
    13081 atgctggcaa ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta
    13141 tctaccgcaa aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc
    13201 tatgtttcat tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat
    13261 ggacgtttta tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa
    13321 ttaatggtcg gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta
    13381 gatgtaaatg ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca
    13441 gagctaatta gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca
    13501 gcggataatc tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa
    13561 atcgatttgt cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt
    13621 gatcgagaga caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt
    13681 gttgataagt ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact
    13741 tatccagaaa aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta
    13801 gaaaaaacag tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa
    13861 acaaatgaag aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa
    13921 ttgacactcc atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc
    13981 atcaagaaat tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga
    14041 cagatgcacg gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag
    14101 catgctgccc aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct
    14161 gatgatctgc aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg
    14221 aaattaaaag agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt
    14281 caaaacagct ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg
    14341 attattcggg agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc
    14401 cgtgaagcaa cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg
    14461 aatgaagcac aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat
    14521 tgtccccatg gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc
    14581 aaacgaatcc aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta
    14641 gcatcccaat caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt
    14701 gaacctgctg atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt
    14761 agaatggctg aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta
    14821 gtgattgcca gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat
    14881 ctggctgagg ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca
    14941 gctgttgttt taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact
    15001 ttttatcctt tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat
    15061 aaggcagggg catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga
    15121 gattattaca gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc
    15181 atttaaacaa aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt
    15241 tttattgaaa agattactta tctactctga ctaagatttt acttcagctt taccgactgc
    15301 ttcgcccggg cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt
    15361 ggtcaaagca acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat
    15421 ttcagcaatc agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg
    15481 aacacccttt agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt
    15541 agaaagaata ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg
    15601 tgcatagact ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga
    15661 gaaaactggg tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa
    15721 tgtttctttt gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt
    15781 tgctgttgcg ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg
    15841 atcaatctta ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat
    15901 gacagcaatg atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat
    15961 tcctggcaaa ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat
    16021 gccagctaat ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat
    16081 tccgaacata gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat
    16141 ttgttcttct tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg
    16201 ggcgatattt gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag
    16261 ctgcatatcg atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag
    16321 gaatccgaat acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat
    16381 atttgagatc cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa
    16441 aacagttggt aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata
    16501 tttgcggaag aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac
    16561 ttgcgcttga taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc
    16621 tgctgcggct gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat
    16681 tcctaaaacg attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat
    16741 acctactgga agaaaatgga aaattgcttc acccggtaac cacaaaaagg cattgacacc
    16801 attccaaaac ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc
    16861 aagtacattt ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt
    16921 aaagatttct gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc
    16981 tgcttttcct tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata
    17041 aaaagtagaa acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc
    17101 ttttacgcta ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac
    17161 aaaacgcata cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc
    17221 atgtaacagc ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt
    17281 aacttgtcta tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag
    17341 cgttttatta aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt
    17401 tcttaaaaat aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga
    17461 agaacgagtg agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag
    17521 aaaattttag cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc
    17581 aaaatttatg gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa
    17641 ggttatattc aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac
    17701 tttcctattt ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt
    17761 gaaacgatca tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact
    17821 ggctgggaga tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa
    17881 gtagttatat tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca
    17941 caagcacaag gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac
    18001 gcgcaaaagg agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt
    18061 cacgatgaca cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt
    18121 ttcgagtatt ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga
    18181 cgccgaaaag tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg
    18241 ctatgatagg atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga
    18301 agaactgaaa gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga
    18361 acgaccattt agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt
    18421 cagcggtgaa ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc
    18481 attcagcaaa ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat
    18541 gcatcgaata gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga
    18601 tggaccagta gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat
    18661 tccatatgaa aagatggatg aattaggtta tggggaattc aaatcacttg tagaataatg
    18721 gaaatacaca aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt
    18781 caagcgattg ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt
    18841 gctacaataa agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc
    18901 tgacaggtgt ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg
    18961 gctaccagat cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa
    19021 aaatttatgt ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc
    19081 tagaagaaaa gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg
    19141 cccttgcaat catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg
    19201 atgtcactta tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct
    19261 tggatctaaa aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg
    19321 aagcaaacac aaaagacgct acagctctat ctgaagcaat ggaagctctc tcggctcttg
    19381 gttacagtga tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga
    19441 caacggatga gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc
    19501 aaatgacaga tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa
    19561 aatctcttcg accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac
    19621 ttcagattta catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt
    19681 acgggcctcc tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg
    19741 taaatattcg tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct
    19801 tgaatgaact ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag
    19861 tggtagaaga gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac
    19921 aagggccaac cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga
    19981 cgacacgggc aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata
    20041 tggaatatta tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc
    20101 aaacagagat cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta
    20161 gaatcgctaa tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga
    20221 aaatcgatcg tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat
    20281 tggattatgt tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag
    20341 tagggttaag tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt
    20401 atgaaccgta tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta
    20461 ctgcttatgc ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt
    20521 ggaggaaaat gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt
    20581 gatttgtgcc aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca
    20641 aaaatcaatc gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg
    20701 gtctattgtg aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg
    20761 gaagagcaag cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat
    20821 actgtgcttt ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca
    20881 tttatcaaga tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta
    20941 ttttatgctc gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat
    21001 gaatatgaag aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg
    21061 gaattatatt cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat
    21121 tttttgttgg aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt
    21181 gtttgtttgg gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata
    21241 gcattagaag gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa
    21301 gttggaaaga caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg
    21361 acgaatatgg ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt
    21421 ggcatggatc aagaaaatgt agaagaactg aaacgaggag caccacgctc agcgcagggg
    21481 aaaatccatt tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca
    21541 tactatacca ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg
    21601 tggaatattt ggattgatga aaataaaata acaaacaatt tgtaaagtta acgctttctt
    21661 tttaaggcga tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt
    21721 aattaagtaa cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat
    21781 cgccaatgaa gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt
    21841 ttgtaaatga cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg
    21901 agctgcaaaa agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt
    21961 tgagtagcaa gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc
    22021 gagtgatgat ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa
    22081 ttgaaaaaat aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta
    22141 taccagatag aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc
    22201 gaatgcttta agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac
    22261 agcattcggt tgattagttg aatcgattgg atggtacaag tttttttgat aaaaaaaggt
    22321 cctgctagcc ggggagctag caggataagg agttaaaaat gaaaaagtgt tgttagggtt
    22381 gtttgttggt atgcttatat aatagtcatt agttgtgaat attttgtgac gaaagttttc
    22441 gaaagttgtt aagtttt
//
LOCUS       DACEND010000038        22113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEND010000038 DACEND010000000
VERSION     DACEND010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.039"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1273..1938)
                     /locus_tag="IW746_001193"
     CDS             complement(1273..1938)
                     /locus_tag="IW746_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078103.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IW746_001194"
     CDS             2064..4100
                     /locus_tag="IW746_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1078104.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IW746_001195"
     CDS             4104..4742
                     /locus_tag="IW746_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078105.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IW746_001196"
     CDS             4770..5198
                     /locus_tag="IW746_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078106.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IW746_001197"
     CDS             5655..6566
                     /locus_tag="IW746_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1078107.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IW746_001198"
     CDS             6783..7220
                     /locus_tag="IW746_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078108.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IW746_001199"
     CDS             7315..8199
                     /locus_tag="IW746_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1078109.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IW746_001200"
     CDS             8423..11590
                     /locus_tag="IW746_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078110.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IW746_001201"
     CDS             11610..12155
                     /locus_tag="IW746_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1078111.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IW746_001202"
     CDS             12183..12638
                     /locus_tag="IW746_001202"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1078112.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IW746_001203"
     CDS             12635..13393
                     /locus_tag="IW746_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1078113.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IW746_001204"
     CDS             complement(13479..14219)
                     /locus_tag="IW746_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078114.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IW746_001205"
     CDS             14280..15533
                     /locus_tag="IW746_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1078115.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IW746_001206"
     CDS             15855..16082
                     /locus_tag="IW746_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1078116.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IW746_001207"
     CDS             16205..16531
                     /locus_tag="IW746_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1078117.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IW746_001208"
     CDS             16599..17312
                     /locus_tag="IW746_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1078118.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18473
                     /locus_tag="IW746_001209"
     CDS             17622..18473
                     /locus_tag="IW746_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078119.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18594..20459
                     /locus_tag="IW746_001210"
     CDS             18594..20459
                     /locus_tag="IW746_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1078120.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20507..21916
                     /locus_tag="IW746_001211"
     CDS             20507..21916
                     /locus_tag="IW746_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1078121.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22024..>22113
                     /locus_tag="IW746_001212"
     CDS             22024..>22113
                     /locus_tag="IW746_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078122.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSML"
BASE COUNT         7446 a         3599 c         4540 g         6528 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgtttt aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc
    17821 cggcagagga gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag
    17881 ttgaattttc gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac
    17941 gagcaaaaga aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc
    18001 cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg
    18061 aactttctga ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa
    18121 acgaagacat gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca
    18181 tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta
    18241 ctcatctgca gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg
    18301 aatttctcta cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga
    18361 tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt
    18421 taacgattca tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt
    18481 tactgtatgg caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt
    18541 tttttcaatc tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata
    18601 atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt
    18661 tagtacattg tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc
    18721 aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag
    18781 tagtgattgg cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg
    18841 cagtttcgga ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta
    18901 tctctagtat atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat
    18961 tcttatcatt ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat
    19021 tgtttgctgc agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag
    19081 ctaaaaagtt caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc
    19141 cagctatcac agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga
    19201 ttctttcacc atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc
    19261 aaagtaaatt agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag
    19321 ttccgcttgt cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg
    19381 ggacggttgc cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa
    19441 tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt
    19501 ggggattcgt accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc
    19561 caatgcttct tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa
    19621 caaaagatac gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa
    19681 tcactgaacc aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt
    19741 gtatttctgg aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca
    19801 gcagtttggt cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt
    19861 caaatgtgac agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta
    19921 cattgatttt aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga
    19981 atgcaggaga accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag
    20041 tactgccatt gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta
    20101 tcgctattga tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta
    20161 tttttccaac tggacatgct gtagggatca caacgactga cggagcggag attttgattc
    20221 atatcgggat ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag
    20281 gggatctcgt caaagcagga gatttattga ttcgttttga catcgaagca attagagcag
    20341 ctggctatag cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac
    20401 ttgaattaga ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag
    20461 gaggaaatca ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct
    20521 gccaaatcaa ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc
    20581 tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat
    20641 tgatcaagaa aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa
    20701 agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg
    20761 gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta
    20821 tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca
    20881 ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat
    20941 cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata
    21001 ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt
    21061 agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt
    21121 cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg
    21181 ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc
    21241 aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga
    21301 ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga
    21361 gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag
    21421 ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa
    21481 cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga
    21541 tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt
    21601 cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga
    21661 tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga
    21721 cggtgtagat ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac
    21781 aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg
    21841 gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa
    21901 tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt
    21961 tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac
    22021 gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt
    22081 ttctcaggaa aatatccagt cggttcgatg ttg
//
LOCUS       DACEND010000039        20779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEND010000039 DACEND010000000
VERSION     DACEND010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.040"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW746_001213"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW746_001213"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1078123.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW746_001214"
     CDS             complement(2260..2511)
                     /locus_tag="IW746_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1078124.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW746_001215"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW746_001215"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1078125.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IW746_001216"
     CDS             3461..3679
                     /locus_tag="IW746_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078126.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IW746_001217"
     CDS             complement(3968..4480)
                     /locus_tag="IW746_001217"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1078127.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW746_001218"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IW746_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1078128.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IW746_001219"
     CDS             complement(6933..7367)
                     /locus_tag="IW746_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1078129.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IW746_001220"
     CDS             complement(7377..8165)
                     /locus_tag="IW746_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1078130.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IW746_001221"
     CDS             complement(8162..9103)
                     /locus_tag="IW746_001221"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1078131.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IW746_001222"
     CDS             complement(9280..9867)
                     /locus_tag="IW746_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1078132.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW746_001223"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IW746_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1078133.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IW746_001224"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IW746_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1078134.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IW746_001225"
     CDS             complement(12784..13719)
                     /locus_tag="IW746_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1078135.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="IW746_001226"
     CDS             13905..14864
                     /locus_tag="IW746_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1078136.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="IW746_001227"
     CDS             complement(14950..16044)
                     /locus_tag="IW746_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1078137.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="IW746_001228"
     CDS             16233..16994
                     /locus_tag="IW746_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1078138.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IW746_001229"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IW746_001229"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1078139.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17769..18524
                     /gene="fabI"
                     /locus_tag="IW746_001230"
     CDS             17769..18524
                     /gene="fabI"
                     /locus_tag="IW746_001230"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1078140.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18634..19647)
                     /locus_tag="IW746_001231"
     CDS             complement(18634..19647)
                     /locus_tag="IW746_001231"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1078141.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19812..20732
                     /locus_tag="IW746_001232"
     CDS             19812..20732
                     /locus_tag="IW746_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1078142.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         5993 a         4464 c         3682 g         6640 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
    17641 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
    17701 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
    17761 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
    17821 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
    17881 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
    17941 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
    18001 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
    18061 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
    18121 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
    18181 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
    18241 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
    18301 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
    18361 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
    18421 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
    18481 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
    18541 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
    18601 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
    18661 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
    18721 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
    18781 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
    18841 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
    18901 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
    18961 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
    19021 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
    19081 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
    19141 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
    19201 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
    19261 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
    19321 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
    19381 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
    19441 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
    19501 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
    19561 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
    19621 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
    19681 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
    19741 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
    19801 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
    19861 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
    19921 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
    19981 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
    20041 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
    20101 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
    20161 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
    20221 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
    20281 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
    20341 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
    20401 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
    20461 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
    20521 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
    20581 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
    20641 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
    20701 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
    20761 taaaaagagc gtgggacaa
//
LOCUS       DACEND010000040        20313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEND010000040 DACEND010000000
VERSION     DACEND010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.041"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            432..1805
                     /gene="glmU"
                     /locus_tag="IW746_001233"
     CDS             432..1805
                     /gene="glmU"
                     /locus_tag="IW746_001233"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1078143.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1899..2870
                     /locus_tag="IW746_001234"
     CDS             1899..2870
                     /locus_tag="IW746_001234"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1078144.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2876..2978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3044..3613
                     /gene="thiT"
                     /locus_tag="IW746_001235"
     CDS             3044..3613
                     /gene="thiT"
                     /locus_tag="IW746_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1078145.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3694..4467)
                     /locus_tag="IW746_001236"
     CDS             complement(3694..4467)
                     /locus_tag="IW746_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078146.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4733..6433
                     /locus_tag="IW746_001237"
     CDS             4733..6433
                     /locus_tag="IW746_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1078147.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6403..6621
                     /locus_tag="IW746_001238"
     CDS             6403..6621
                     /locus_tag="IW746_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078148.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6641..7531
                     /locus_tag="IW746_001239"
     CDS             6641..7531
                     /locus_tag="IW746_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1078149.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKTREQVQNIE"
     gene            7549..7833
                     /locus_tag="IW746_001240"
     CDS             7549..7833
                     /locus_tag="IW746_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078150.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            8320..8934
                     /gene="gmk"
                     /locus_tag="IW746_001241"
     CDS             8320..8934
                     /gene="gmk"
                     /locus_tag="IW746_001241"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1078151.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            8939..9244
                     /locus_tag="IW746_001242"
     CDS             8939..9244
                     /locus_tag="IW746_001242"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1078152.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            9497..11905
                     /gene="priA"
                     /locus_tag="IW746_001243"
     CDS             9497..11905
                     /gene="priA"
                     /locus_tag="IW746_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1078153.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            11920..12411
                     /gene="def"
                     /locus_tag="IW746_001244"
     CDS             11920..12411
                     /gene="def"
                     /locus_tag="IW746_001244"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1078154.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            12404..13342
                     /locus_tag="IW746_001245"
     CDS             12404..13342
                     /locus_tag="IW746_001245"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1078155.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            13342..14700
                     /gene="rsmB"
                     /locus_tag="IW746_001246"
     CDS             13342..14700
                     /gene="rsmB"
                     /locus_tag="IW746_001246"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1078156.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            14713..15453
                     /locus_tag="IW746_001247"
     CDS             14713..15453
                     /locus_tag="IW746_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1078157.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            15450..17519
                     /gene="pknB"
                     /locus_tag="IW746_001248"
     CDS             15450..17519
                     /gene="pknB"
                     /locus_tag="IW746_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1078158.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            17684..18583
                     /gene="rsgA"
                     /locus_tag="IW746_001249"
     CDS             17684..18583
                     /gene="rsgA"
                     /locus_tag="IW746_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1078159.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            18596..19246
                     /locus_tag="IW746_001250"
     CDS             18596..19246
                     /locus_tag="IW746_001250"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1078160.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            19247..19885
                     /locus_tag="IW746_001251"
     CDS             19247..19885
                     /locus_tag="IW746_001251"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1078161.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20040..>20313
                     /locus_tag="IW746_001252"
     CDS             20040..>20313
                     /locus_tag="IW746_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1078162.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDD"
BASE COUNT         6821 a         3672 c         4391 g         5429 t
ORIGIN      
        1 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatct agaggttgtg
       61 aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt
      121 gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg
      181 taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt
      241 ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga
      301 taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca
      361 cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag
      421 gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca
      481 tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata
      541 tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag
      601 cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat
      661 tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa
      721 cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg
      781 aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa
      841 caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa
      901 aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg
      961 acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat
     1021 actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc
     1081 aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa
     1141 atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg
     1201 attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag
     1261 caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt
     1321 caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa
     1381 gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg
     1441 aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca
     1501 aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca
     1561 atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg
     1621 tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta
     1681 aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg
     1741 caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa
     1801 actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt
     1861 agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc
     1921 caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag
     1981 cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag
     2041 taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc
     2101 cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg
     2161 caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac
     2221 gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa
     2281 caagaatgct gactttggac cttcatgcag tccaaatcca aggtttcttt gatattccag
     2341 tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag
     2401 gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag
     2461 cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg
     2521 cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata
     2581 tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa
     2641 ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag
     2701 aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta
     2761 aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta
     2821 tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact
     2881 cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta
     2941 tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc
     3001 ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt
     3061 ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca
     3121 gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag
     3181 aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt
     3241 gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac
     3301 ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa
     3361 tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata
     3421 tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa
     3481 ttcttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac
     3541 gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg
     3601 acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct
     3661 tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc
     3721 ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat
     3781 ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat
     3841 tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc
     3901 ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc
     3961 ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact
     4021 agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt
     4081 aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt
     4141 tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat
     4201 cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga
     4261 ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc
     4321 atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat
     4381 actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc
     4441 aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt
     4501 ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa
     4561 aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt
     4621 ttatttaaag gatgttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga
     4681 ctcatgacct ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat
     4741 aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca
     4801 accccttcca ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa
     4861 aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa
     4921 aagtagagga gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa
     4981 aaaatttttt cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt
     5041 tttatttcaa tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca
     5101 aggaaccaca caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg
     5161 aggagcacac ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa
     5221 ctttgagatt atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg
     5281 agatatgaaa aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt
     5341 accagaaatg gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat
     5401 gacgaaaaca gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa
     5461 agacaatttg gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc
     5521 tactaaactg ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga
     5581 aataccagat tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat
     5641 cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat
     5701 taacaaagga gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa
     5761 aattcgtatg ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag
     5821 ctttctgctt tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat
     5881 gaaaaaacga ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc
     5941 aagaacatac tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc
     6001 ttttttcaaa agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt
     6061 accagagaca tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc
     6121 caatgaaatc tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag
     6181 tatgccggat gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta
     6241 tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct
     6301 gctacaagga aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac
     6361 attactgatc ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca
     6421 tatgaaatcg tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa
     6481 ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca
     6541 gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa
     6601 aaagcaatag aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt
     6661 tggcaaagcc atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa
     6721 ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga
     6781 ttttcgtcag cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct
     6841 agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact
     6901 tatcactcaa ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat
     6961 tagtcggatg gcggaacgct tcgcgctgga cgaaaacttc gtaacggtag aagaaaaacc
     7021 ggaagagagc atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga
     7081 ttctattgag gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa
     7141 tactgaagaa ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa
     7201 ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga
     7261 gcaacggttg ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact
     7321 ggatcgtctg gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt
     7381 aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc
     7441 caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa
     7501 aaccagagaa caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat
     7561 gacgtaacgt tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa
     7621 aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt
     7681 attttgatca acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac
     7741 acagaagagg tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt
     7801 gtagtgaata ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat
     7861 aaataatcag aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt
     7921 tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat
     7981 tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt
     8041 cttcatactc gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt
     8101 gcatctgcga taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg
     8161 ttcttattac ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt
     8221 accaatttct agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata
     8281 aacgataagt acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta
     8341 atcgtgttgt ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag
     8401 agtgaggata atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg
     8461 gaggtcgaag gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa
     8521 gctggagaga tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca
     8581 tatgtcaaca agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga
     8641 gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat
     8701 ttagcggaat taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa
     8761 gaacgaatga aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt
     8821 gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat
     8881 tttcgagtag atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat
     8941 gatgttaaaa ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt
     9001 gattttagca agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc
     9061 ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt
     9121 caatgatcca catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca
     9181 agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat
     9241 gtaataaaga aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt
     9301 caaacattcg ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt
     9361 cttttttttc ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc
     9421 catcgatctc tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa
     9481 aaggaggaaa cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca
     9541 gaccgatcag ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg
     9601 gatgcgagta gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc
     9661 tatccaaaaa tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga
     9721 ccttgctccg gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac
     9781 ttacgcattc aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata
     9841 tgataaattg atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg
     9901 agaagtgatt tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg
     9961 gcgccaagag caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac
    10021 gattcgtttg gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt
    10081 acggcaaaat gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga
    10141 agaaccaatt gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa
    10201 agaaaagggc tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg
    10261 agtatttgac caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca
    10321 aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg
    10381 cagcgggaaa acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac
    10441 ggctattatg ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg
    10501 acgcttcgga gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga
    10561 tgaatggcgc aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat
    10621 ttttgccccg ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta
    10681 caaacaagat gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata
    10741 tcatcattgc ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc
    10801 tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct
    10861 tccagctatt gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt
    10921 ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct
    10981 actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct
    11041 tccatgtcct aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg
    11101 ccattattgc ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa
    11161 aatccgctac tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc
    11221 gcaagctagg attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca
    11281 gattttacaa aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc
    11341 taaaggtttg gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct
    11401 gaatttaccg gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg
    11461 acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca
    11521 ttacgccata gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca
    11581 tgtacggcat cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca
    11641 tcctgaagaa caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt
    11701 gttaagccct caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa
    11761 ccgatattat tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt
    11821 ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga
    11881 caacgaacca ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc
    11941 caccctaatg ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca
    12001 atcgctttat tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct
    12061 gctcctcaag taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa
    12121 tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt
    12181 tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt
    12241 tattatgatc gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc
    12301 ttccagcatg aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag
    12361 atcccggaag aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt
    12421 tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga
    12481 gatccaggca gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc
    12541 gacaccggta aaagaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat
    12601 ctccggttcg ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc
    12661 ggcatttggc caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa
    12721 tgtccatgct tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat
    12781 gaatggggag aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg
    12841 cggaatctat gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt
    12901 tgaaaagctt agccttttag gaagaaagct attgctagaa acattgccca atattttgga
    12961 tggacaaaaa ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg
    13021 ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg
    13081 aatgcgtcct tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc
    13141 ggttgaagta ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa
    13201 aaaaaatcta tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc
    13261 agctggtaaa ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca
    13321 gattggtcag caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga
    13381 tatatggcat tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg
    13441 ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa
    13501 ttgatctatg gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc
    13561 gctaaagcaa aaaaagtgga tgactgggtc aagacgcttc tttatctttc tgtataccaa
    13621 ttagaatttt tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca
    13681 aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag
    13741 cgtcaaggag caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa
    13801 atcagcctgc ctctatggat gactaaacga ctggtggatc aaatcggata cgaagaaact
    13861 gaaaagctcg gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc
    13921 cgactttcca gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa
    13981 agccaagtca gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg
    14041 ttccacgatg gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg
    14101 caaatcgagc ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg
    14161 catattgctt cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac
    14221 gaacacaaag tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc
    14281 tacgctcaaa agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat
    14341 cggattttag tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt
    14401 cgttataaaa aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta
    14461 gaaagctgcg caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc
    14521 ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag
    14581 aggattgatg ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact
    14641 ttgtatccgc atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa
    14701 tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat
    14761 caagactatg ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac
    14821 gggatgggtg gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc
    14881 gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa
    14941 tcgattcaag aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac
    15001 ggtatgggaa caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat
    15061 gttggggata gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat
    15121 cattctttag tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac
    15181 catccaagga aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat
    15241 gtttctactc atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg
    15301 aacatgctgt ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat
    15361 aaagtaactg agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt
    15421 cttcttatcg aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc
    15481 gttatcaaat cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg
    15541 atttaatctt ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc
    15601 agactgcgat tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa
    15661 acattgttag tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat
    15721 acgtcaaagg aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa
    15781 cagcagtaaa tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa
    15841 tcatccaccg agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa
    15901 tcacggattt cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga
    15961 tgctaggatc tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc
    16021 agtcagatat ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt
    16081 ttgatggtga atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta
    16141 taaaagcact tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg
    16201 ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag
    16261 tattatcacc agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa
    16321 caaaagttat cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc
    16381 ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg
    16441 atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt
    16501 taggaatcgg aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg
    16561 atgtttctga tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag
    16621 tagcaacaaa aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga
    16681 cagacccggc agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta
    16741 gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag
    16801 caagtttgaa agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt
    16861 cagaggtaga aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc
    16921 ctaaaacaga cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta
    16981 gtcatgtagg agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata
    17041 tcaataatat tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca
    17101 gtcaggatcc agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa
    17161 gccaaggaac agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa
    17221 gttatttagc tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca
    17281 cggtggataa agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag
    17341 gaatggttgt gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa
    17401 aagactcttc agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa
    17461 aagaagaatc ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag
    17521 tgctgaataa aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt
    17581 gtttctatct cttttgatca attttcaagc catatacttt gagaagtttg catggaataa
    17641 cacctacttt cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg
    17701 acaaatcaga aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca
    17761 aacaagagga agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt
    17821 gatctttgag agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa
    17881 tcaattagtt cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt
    17941 agagcctgca ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc
    18001 gatcgaaccg attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga
    18061 agagatcaag caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg
    18121 tgatacgaca gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc
    18181 tggagcagga aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg
    18241 agagatttct gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc
    18301 tatctatggc gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat
    18361 tgaagcagga gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa
    18421 atttagagag tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac
    18481 aggtggtatc gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa
    18541 aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa
    18601 gatagcacca tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt
    18661 ggaaaaagga ggcgcagatt acatccacgt agatgtaatg gatggccaat ttgtcccaaa
    18721 tatcacatta gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga
    18781 cgtccatctg atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc
    18841 agatatcatt actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac
    18901 aaaaagctta ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat
    18961 caaacatgtt ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg
    19021 cggacaaagc tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag
    19081 agagaacaat tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa
    19141 aatctgtaaa gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga
    19201 aaatccagtc ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt
    19261 agctgctgga ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta
    19321 tgtcgggata gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc
    19381 agtgggtgat tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa
    19441 agagtatacc cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa
    19501 aacatttgaa cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt
    19561 ggatcatctt ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca
    19621 tcagcttatt atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat
    19681 agtaagacag ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa
    19741 tcttacatta aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc
    19801 ttatgcaagt aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat
    19861 tgcagttatt caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct
    19921 aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg
    19981 tttcacggca tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg
    20041 tgaagaagtt caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg
    20101 tgacgatact aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc
    20161 aatccatcgt taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt
    20221 gggtacgaaa tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc
    20281 ggctaaaaga aaaaatcgat agctatgatg acg
//
LOCUS       DACEND010000041        19905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEND010000041 DACEND010000000
VERSION     DACEND010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.042"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..996)
                     /locus_tag="IW746_001253"
                     /pseudo
     CDS             complement(13..996)
                     /locus_tag="IW746_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(1001..1648)
                     /locus_tag="IW746_001254"
     CDS             complement(1001..1648)
                     /locus_tag="IW746_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1078163.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IW746_001255"
     CDS             complement(1733..2365)
                     /locus_tag="IW746_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1078164.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW746_001256"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IW746_001256"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1078165.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IW746_001257"
     CDS             complement(2929..3666)
                     /locus_tag="IW746_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1078166.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IW746_001258"
     CDS             complement(3663..4652)
                     /locus_tag="IW746_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1078167.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IW746_001259"
     CDS             4910..5452
                     /locus_tag="IW746_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078168.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IW746_001260"
     CDS             5469..6554
                     /locus_tag="IW746_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078169.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IW746_001261"
     CDS             6566..7372
                     /locus_tag="IW746_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078170.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IW746_001262"
     CDS             7373..8200
                     /locus_tag="IW746_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078171.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IW746_001263"
     CDS             8197..9270
                     /locus_tag="IW746_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1078172.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IW746_001264"
     CDS             9698..12394
                     /locus_tag="IW746_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078173.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IW746_001265"
     CDS             12471..12959
                     /locus_tag="IW746_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1078174.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IW746_001266"
     CDS             12971..13465
                     /locus_tag="IW746_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078175.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IW746_001267"
     CDS             13486..14295
                     /locus_tag="IW746_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078176.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IW746_001268"
     CDS             14292..15113
                     /locus_tag="IW746_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1078177.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IW746_001269"
     CDS             15164..17263
                     /locus_tag="IW746_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1078178.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IW746_001270"
     CDS             17260..18378
                     /locus_tag="IW746_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1078179.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IW746_001271"
     CDS             18417..19295
                     /locus_tag="IW746_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1078180.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19441..19638
                     /locus_tag="IW746_001272"
     CDS             19441..19638
                     /locus_tag="IW746_001272"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078181.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         6072 a         3455 c         4254 g         6124 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagctagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatacgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaat
//
LOCUS       DACEND010000042        19882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEND010000042 DACEND010000000
VERSION     DACEND010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.043"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..703
                     /locus_tag="IW746_001273"
     CDS             <1..703
                     /locus_tag="IW746_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1078182.1"
                     /translation="GRWAQNRRLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFST
                     ENWKRPKDEVSFLMQLPVDFFDTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQ
                     TKNNTGMVLNFALNYGSRAEIVTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPK
                     ELQDPELMIRTSGEERISNFLLWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRR
                     FGGVKKTEDEKGDQS"
     gene            700..1500
                     /locus_tag="IW746_001274"
     CDS             700..1500
                     /locus_tag="IW746_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1078183.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1661..2929
                     /gene="rseP"
                     /locus_tag="IW746_001275"
     CDS             1661..2929
                     /gene="rseP"
                     /locus_tag="IW746_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1078184.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            2997..4706
                     /locus_tag="IW746_001276"
     CDS             2997..4706
                     /locus_tag="IW746_001276"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1078185.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            4918..9270
                     /locus_tag="IW746_001277"
     CDS             4918..9270
                     /locus_tag="IW746_001277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1078186.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9414..9887
                     /gene="rimP"
                     /locus_tag="IW746_001278"
     CDS             9414..9887
                     /gene="rimP"
                     /locus_tag="IW746_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1078187.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            9911..11086
                     /gene="nusA"
                     /locus_tag="IW746_001279"
     CDS             9911..11086
                     /gene="nusA"
                     /locus_tag="IW746_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1078188.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11108..11401
                     /locus_tag="IW746_001280"
     CDS             11108..11401
                     /locus_tag="IW746_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1078189.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11398..11709
                     /locus_tag="IW746_001281"
     CDS             11398..11709
                     /locus_tag="IW746_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1078190.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11722..14028
                     /gene="infB"
                     /locus_tag="IW746_001282"
     CDS             11722..14028
                     /gene="infB"
                     /locus_tag="IW746_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1078191.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14055..14402
                     /gene="rbfA"
                     /locus_tag="IW746_001283"
     CDS             14055..14402
                     /gene="rbfA"
                     /locus_tag="IW746_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1078192.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14510..14731)
                     /locus_tag="IW746_001284"
     CDS             complement(14510..14731)
                     /locus_tag="IW746_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1078193.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15179..17542
                     /locus_tag="IW746_001285"
     CDS             15179..17542
                     /locus_tag="IW746_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ1078194.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            17910..18833
                     /gene="truB"
                     /locus_tag="IW746_001286"
     CDS             17910..18833
                     /gene="truB"
                     /locus_tag="IW746_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1078195.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18837..19778
                     /gene="ribF"
                     /locus_tag="IW746_001287"
     CDS             18837..19778
                     /gene="ribF"
                     /locus_tag="IW746_001287"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1078196.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6817 a         3489 c         4373 g         5203 t
ORIGIN      
        1 tggtcgttgg gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga
       61 aacagtaaag aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta
      121 tgccttttcg actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact
      181 acctgtcgac ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca
      241 tgtcatgggt tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat
      301 tgagcaaaca aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag
      361 agcagagatc gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca
      421 cgcagaagaa atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa
      481 agagttacaa gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt
      541 tctgttatgg cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt
      601 tgatggtgcg catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg
      661 cggtgtaaaa aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca
      721 gcagctgtgg cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag
      781 cttacagcag caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg
      841 acactcttaa gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca
      901 aaagaaagat ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt
      961 atagttatga ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa
     1021 gcaggttttc ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat
     1081 gcacgaacag aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat
     1141 atcggagcat atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg
     1201 cccaacaaaa cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt
     1261 ctttatttat tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt
     1321 ctgatgacga ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc
     1381 aaacgtcatt atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta
     1441 gaccgtttcg acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag
     1501 taagtatacc tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta
     1561 tattgatact attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt
     1621 ttataaaaaa cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt
     1681 tatcatcgtt tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa
     1741 acgatcagga atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca
     1801 tcaagcaaaa gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg
     1861 aatggcaggc aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt
     1921 actaaattct gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa
     1981 tgctatcccg atggaattga gccgttatga tttagaagat gagctgacta tcactggtta
     2041 tgtgaatgga gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat
     2101 tgaaaacgac ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa
     2161 attgtggcag cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat
     2221 cgtgttattc atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg
     2281 cgtaggagag atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga
     2341 ggtagtcagt gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac
     2401 gaaaaatcca ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt
     2461 cgacgtgaca ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa
     2521 agccccaatg aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag
     2581 cggttcattg gaaattttca aagccttagg atcactattt acaggtttca gtttagataa
     2641 attaggcgga cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac
     2701 aactgttatt agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc
     2761 gattcctgcc ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa
     2821 accattaagt caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt
     2881 gttgatggtg ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac
     2941 aaatagggta taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga
     3001 gacaatcaaa aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa
     3061 gccatcaaat cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat
     3121 atctgccatt agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg
     3181 aaaaaatcgg agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag
     3241 aatccgggcg ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc
     3301 gaaaaatgat ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa
     3361 tcaattcata taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg
     3421 aaaaacgacc tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt
     3481 ctttccatgc aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata
     3541 ccgaagtatt caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa
     3601 tgggcgggaa agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct
     3661 gttattcgac tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat
     3721 caaaaaaatc gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat
     3781 caattgacga agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat
     3841 tgttcattgc ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg
     3901 acgtaaaact gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag
     3961 cacaaaaata tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg
     4021 taaaagtata tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg
     4081 aaacaggatt ccactataaa aatgtgaatc cagaacgcga tttcactgtg ctttcttatg
     4141 aagatcttcg ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct
     4201 ttactcgagg aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta
     4261 tgggagcaac tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg
     4321 gtatcggtgt aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa
     4381 tcaattggcc aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag
     4441 acgaatacca aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg
     4501 aagtactggt tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga
     4561 ttggctgtcc aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga
     4621 aaatcaagaa aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac
     4681 caatcctctt gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga
     4741 atcagctgtt tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg
     4801 cagttccaat tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa
     4861 agaagaggat aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg
     4921 tcagaaaaac gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa
     4981 aaaaatcatc cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg
     5041 cgtttatggg aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca
     5101 ttgactcagc atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta
     5161 gcagatgacc agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa
     5221 aatcagcaat gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata
     5281 aaagatcgta aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa
     5341 caatacttgc cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc
     5401 gaaccagaag tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa
     5461 caagaacagg cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa
     5521 aagaaaaagg aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga
     5581 aatattccag ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga
     5641 gtcacacttg aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag
     5701 atcttgactt tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac
     5761 ggcgaaaaag atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga
     5821 ggaagtattc aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata
     5881 gaagtcaaac atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt
     5941 catgttcata gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca
     6001 caagcaggaa aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc
     6061 tttccggaag cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa
     6121 gcgaatgtcg tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt
     6181 gaagccacat acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg
     6241 atcatcgagc tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa
     6301 tttatcgatc caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat
     6361 gaaatggttc gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca
     6421 aaagatacaa ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc
     6481 tacgcaaggt atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct
     6541 agatatctgt atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt
     6601 gttagcttgg aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca
     6661 tggatctttg tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat
     6721 gagcatatcg gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta
     6781 gcaaaaaatc aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa
     6841 tacttcgaac gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta
     6901 ttgatcggct cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta
     6961 gaagaggcaa gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc
     7021 tatgcgcctt tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc
     7081 caaaatttgg tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta
     7141 cattatctga acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga
     7201 gcgaatccgt tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg
     7261 ctaactgcat ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg
     7321 aataaaatcg cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg
     7381 aagattccag gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg
     7441 tacggagatc cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt
     7501 aatggtaatg gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat
     7561 gaagatggct atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg
     7621 acaggaatca ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat
     7681 tccgagtttt ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt
     7741 ccaaaatgtg gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta
     7801 ggattccatg gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca
     7861 gaagcccata actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg
     7921 attggtacag tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat
     7981 aatctgcagt ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa
     8041 cggacaaccg gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat
     8101 gattttacgc cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat
     8161 tttgatttcc actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac
     8221 ccaactgtta ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg
     8281 gatgacccgg aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa
     8341 caaatttatt ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga
     8401 gggatgttag aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta
     8461 tctcatggta cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg
     8521 acactggcgg aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga
     8581 ttagatagcg ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag
     8641 atccctgatg aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac
     8701 tggtatatcg attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct
     8761 tacgtattga tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac
     8821 tgtgcatact tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa
     8881 gatgctgtga aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa
     8941 gaaaagaatc aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa
     9001 tttggaatga ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg
     9061 ttgatcgcac catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt
     9121 gaagcaagaa aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt
     9181 tcaaaaacat tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa
     9241 aatcaattat cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt
     9301 aagcagagta tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt
     9361 cgctcactct ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta
     9421 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9481 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9541 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9601 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9661 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9721 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9781 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9841 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
     9901 tcagaaaaaa atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg
     9961 gatctcaaaa gaaatcgtga tcgatgcatt agaagcagca ttggtttctg catacaaacg
    10021 tcactatggc caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca
    10081 tgtctatgcg gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt
    10141 gaaagatgca cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt
    10201 aacaccaaaa gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg
    10261 tgtacgtgaa gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat
    10321 catgcaagga atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat
    10381 tgaggcagtt ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg
    10441 aatcaaagtt tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag
    10501 ccgcagccat ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga
    10561 tggaatcgtt gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt
    10621 tcgctcaaat gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg
    10681 agttcaagca atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga
    10741 agatccagct gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt
    10801 cgatgagaca aattcaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc
    10861 aatcggtaaa cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga
    10921 tatcaaatca gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac
    10981 agaagaactg catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac
    11041 aattgcattc aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg
    11101 caaagagatg aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa
    11161 acctaaaaaa gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc
    11221 aactggaaaa atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca
    11281 agcttgggat aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta
    11341 tcaagaactt cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg
    11401 aatacagtta atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa
    11461 ctaatcacgg gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg
    11521 tttgtggcaa ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat
    11581 tatgaagttc cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa
    11641 cctcgcaagg tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt
    11701 gaaggttagg aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc
    11761 aaaaaatcta gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac
    11821 catatgggtg cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca
    11881 agcacgaaac aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac
    11941 gatcaacgta aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat
    12001 caagatcgcg gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa
    12061 aataatacgg gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa
    12121 aaccgtacta acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat
    12181 aaccgtaatc gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca
    12241 gctgtaccac cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg
    12301 aacgtagcag atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta
    12361 tttatgcttg gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg
    12421 ttagcgacgg actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc
    12481 gataaattct tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta
    12541 gtaacgatta tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat
    12601 tcacgtgtaa caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc
    12661 gatattgatg gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt
    12721 atgcgtgcac gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat
    12781 ggcgtcatgc cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc
    12841 atcgtcgctg taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa
    12901 ttaagtgagt atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc
    12961 tcagcgaaat tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa
    13021 gtagaagatt taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt
    13081 cttgataaag gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt
    13141 ggtgacccaa tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt
    13201 ggccgtcgcg ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat
    13261 gtacctcaag caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg
    13321 ggtgaagaac gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact
    13381 ttggacaact tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc
    13441 aaagcagatg tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg
    13501 gaaggtgtcc gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta
    13561 accttagcag cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag
    13621 gcaaaagtgc aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa
    13681 gccatcgaag aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa
    13741 atcaccggtc aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca
    13801 ggtgcctatg taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac
    13861 ggaatcgtaa tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa
    13921 gaagtcaaaa tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta
    13981 gatgacgtaa ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa
    14041 ggaggccaat cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag
    14101 aagtcaatga tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca
    14161 cagatgtgaa agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg
    14221 acttagcatc agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc
    14281 gtcgagagtt agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg
    14341 atgaatcagt tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact
    14401 aatcaaaaaa tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt
    14461 aagacgtatt acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa
    14521 agagaaaaag aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca
    14581 tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag
    14641 tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg
    14701 tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc
    14761 cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc
    14821 tgggatacca attcacatat tgacaaagat aacagagact tttacataaa acggatcaat
    14881 caggaaagga attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg
    14941 tcattcagca aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata
    15001 acgacaagtt ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt
    15061 ttatacctgt tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag
    15121 gtttcaattg ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat
    15181 ggattattct agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta
    15241 tctttctgtt gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc
    15301 agaagatgtc aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat
    15361 ttatgtccat ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg
    15421 gccaggacac ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga
    15481 gatctatcca gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc
    15541 atttcctgga ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg
    15601 cggagagctt ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt
    15661 aatctctgct gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg
    15721 gttctcaaat gtctttatca atccagtgat agatggagct gttttaccga tccttcattt
    15781 aaatggtgca aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc
    15841 aaattacttt aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa
    15901 attaaatcct gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca
    15961 aaaagaagct cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat
    16021 cgtacgcaca ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg
    16081 aaccttccga gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga
    16141 tgctttactc cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg
    16201 tattttagaa gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc
    16261 tatcaccaat ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac
    16321 tttgcaattt gaaaaactag gctccgttaa agcagaagat atgaccgaac ttggtaaatt
    16381 cgttcgcgag atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac
    16441 aaaatcaaac cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat
    16501 cgaaccagaa aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc
    16561 ggaacaccaa gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt
    16621 tgcaagttac gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg
    16681 gatacggaaa tcacatgacc tttcttggcg aaatgattat ccttcgctaa atctcattgc
    16741 aagctcaaca gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt
    16801 gacacatttg gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa
    16861 tacattgctc gcagtcatgg ataaagcatt ccactcgtca gaaaaaatca atttgatcat
    16921 ctcttccaaa catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa
    16981 tgaaggcttg aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt
    17041 cattgctgct gcaggaacgg aatctaactt agaagcatta gcagctgcaa ctttgctgct
    17101 ggaagaattc ccaaaactaa aaattcggtt cattaatgtc gtagatttat tgaaattacg
    17161 tcatccaagt caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa
    17221 agacaaaccg atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt
    17281 tgatcgtcat aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac
    17341 accatttgac atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc
    17401 tttaaaaatc aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca
    17461 agaacatcaa aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa
    17521 atggaaaaat cttgatcaat aaaatgataa aatcgatccc gcaaaacaga attttttaca
    17581 ttgattaaga aactgaaaaa ataaataaaa aaaacggaaa aaagactgga agaatggaca
    17641 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
    17701 atccaatcaa atggtgcaaa gaaaaaatct tcctttctat tccctccttt aaaaaaacat
    17761 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
    17821 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
    17881 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaccttta tggaaagaac
    17941 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
    18001 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcctatt tgtattggaa
    18061 aagccacaaa agttatcgag tatttgacag attctgggaa aatctatgta ggcgagataa
    18121 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
    18181 tagatatgtc tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga
    18241 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
    18301 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
    18361 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
    18421 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
    18481 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag
    18541 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
    18601 tgctgccaat agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat
    18661 ggcagaaagt aaaaaacggt atgagacttg attatactgt atttgcgctc ccagaaatgc
    18721 cgtcagaaga aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc
    18781 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
    18841 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
    18901 tagtactggg attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta
    18961 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
    19021 cgattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
    19081 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
    19141 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
    19201 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
    19261 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
    19321 aaggtgaaaa aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag
    19381 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
    19441 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt
    19501 tgcctaaaga aggtgtttat gtatcggaaa taaaaattgg ggaaacttgg tatccagcca
    19561 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt
    19621 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat
    19681 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaaaagg
    19741 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
    19801 gtttatgtat aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct
    19861 gtgacaaaag tcttgtcaca gc
//
LOCUS       DACEND010000043        19675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEND010000043 DACEND010000000
VERSION     DACEND010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.044"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..571
                     /locus_tag="IW746_001288"
     CDS             2..571
                     /locus_tag="IW746_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078197.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(749..1189)
                     /locus_tag="IW746_001289"
     CDS             complement(749..1189)
                     /locus_tag="IW746_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1078198.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1347..2111
                     /gene="map"
                     /locus_tag="IW746_001290"
     CDS             1347..2111
                     /gene="map"
                     /locus_tag="IW746_001290"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1078199.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2143..3066
                     /locus_tag="IW746_001291"
     CDS             2143..3066
                     /locus_tag="IW746_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1078200.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3142..4281
                     /locus_tag="IW746_001292"
     CDS             3142..4281
                     /locus_tag="IW746_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1078201.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4274..5074
                     /locus_tag="IW746_001293"
     CDS             4274..5074
                     /locus_tag="IW746_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1078202.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5074..5901
                     /locus_tag="IW746_001294"
     CDS             5074..5901
                     /locus_tag="IW746_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1078203.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5879..6613
                     /locus_tag="IW746_001295"
     CDS             5879..6613
                     /locus_tag="IW746_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1078204.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6713..7579
                     /gene="rfbA"
                     /locus_tag="IW746_001296"
     CDS             6713..7579
                     /gene="rfbA"
                     /locus_tag="IW746_001296"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1078205.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7593..8165
                     /gene="rfbC"
                     /locus_tag="IW746_001297"
     CDS             7593..8165
                     /gene="rfbC"
                     /locus_tag="IW746_001297"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1078206.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8187..9215
                     /gene="rfbB"
                     /locus_tag="IW746_001298"
     CDS             8187..9215
                     /gene="rfbB"
                     /locus_tag="IW746_001298"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1078207.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9313..10164
                     /gene="rfbD"
                     /locus_tag="IW746_001299"
     CDS             9313..10164
                     /gene="rfbD"
                     /locus_tag="IW746_001299"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1078208.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10198..12231
                     /locus_tag="IW746_001300"
     CDS             10198..12231
                     /locus_tag="IW746_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078209.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12275..13554
                     /locus_tag="IW746_001301"
                     /pseudo
     CDS             12275..13554
                     /locus_tag="IW746_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            13764..14570
                     /locus_tag="IW746_001302"
     CDS             13764..14570
                     /locus_tag="IW746_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078210.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14582..15802
                     /locus_tag="IW746_001303"
     CDS             14582..15802
                     /locus_tag="IW746_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078211.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15792..17378
                     /locus_tag="IW746_001304"
     CDS             15792..17378
                     /locus_tag="IW746_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1078212.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17417..19555
                     /locus_tag="IW746_001305"
     CDS             17417..19555
                     /locus_tag="IW746_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1078213.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6232 a         3287 c         4176 g         5980 t
ORIGIN      
        1 tatggcaaga aaaaagacga ttactagaga acagattttg gcagctgctt atgaagtagc
       61 tgccaccgat ggatttacac gatttactgc gagaaacatc gcgaacaaaa tgaattgttc
      121 cacgcaaccg atttatcttg agtttaaaaa tatggatgac ttgagacaag ctctttttga
      181 gcagatctat gatcatttac aatatgaagt gttcactgtt gagcatacag ggaatacgat
      241 tatcgatctt gcttttaatt atatccattt tgcaagaaga gagaagaagt tgtatcgttc
      301 actgtactta gaagaatatg gtggaggaaa agagatgcag aatttctctt atacttactt
      361 tgtgaataca gtgaaacgcg atccagctta tgtgaatctt tcagaagagc agatcgaatc
      421 tttgtataat gggacatgga tcatagctac tgggatcgca gctttgatgt cttcaagcat
      481 catccatcca actgatgaac agattgaaaa gatgatccaa gacagtatag atgctatctt
      541 gaaattcaaa gaaccaatcg aaatcgattg aatatactca aaagttccac aaatcagtag
      601 gtaacaagag atgtgtcaaa ataaggaaaa gttcgttttt cttacagcgt atgcattttt
      661 aactcccgaa gaactcttcc atgaatgcaa tgaataaaag agccatacag attgaataaa
      721 tctggatggc tcttttattg caagtatatt aaatttttgc gactagttct ttagcgaaag
      781 cttctaaatt ttcgatatct tcttcttcag cattcagatc cactttaaca ttttcggctc
      841 cttttgatgc gcctgttttt gcaaaagcag catcgaaatc atccaccgat ttgcagaatt
      901 catcgtaaaa agtatcacca gatccgcaga caccgtaaat ttttcccaat agatccaatt
      961 cttgcaagtc ttcataaaag tctacgatct catcaggcag atcgccatca tcgtatgtat
     1021 aagtagctac tacgcaaata tctgcctcct cgaattcatc tgcatctact tgggtacatt
     1081 cgttgatttc gacttcgata tccagatttt caaatgcttc tgcaacaata tcggcgattt
     1141 cttcagtgtt tcctgtcatg ctagcatata cgatttttgc taaagccatg atgtgcctcc
     1201 tcgtttataa ttatttaaca attatgaatt ataccaaaac atcattgaaa agaaaataaa
     1261 acctagagac acctggggaa tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga
     1321 gggatttgta aaaaggagac gaaaatatga ttactttaaa atcagcacgt gaaattgaaa
     1381 tgatggcaga atctggagct ttattagcag atgtacataa aaatctgcgt gattttataa
     1441 aaccaggaat cacaagttgg gatattgagg tatttgtcag aaactatatc gagagtcatg
     1501 gcggaattgc tgcgcaaatt ggctttgaag ggtatgaata tgcgacttgt atcagtataa
     1561 atgatgaaat ttgtcatgga ttccctcgta aaaagccttt gaaaaacggt gatttggtaa
     1621 aagtcgatat gtgtatcgat ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag
     1681 ttggagaatc tacacctgag atcgaccgat tgatggaagt aacgaagaaa gctttgtaca
     1741 tcggtattga gcaagcacaa gtagggaatc ggattggtga catcggtcat gctatccaaa
     1801 cttatgtaga aggcgaaaat ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc
     1861 ctacgattca tgaagaacca gcagttcctc attacggaga agctggaaaa gggttgcggt
     1921 tgaaagaagg catggttatc acgatcgaac ctatggttaa taccggaact tggaaaatga
     1981 aaatggatcc taacggctgg actgcgtata caagagacgg cggactttct tgccaatatg
     2041 aacatacatt ggctattact aaagatggtc caagaatttt gacatcacaa ggagaagaag
     2101 gcacttattg atctatccag tcaataggta cgggagaggt acatgaaagt ttggaacaaa
     2161 ataaaacaaa atgaaaatct gatgcgcttt attgagacga ctcaaaagcg catcactgat
     2221 tcagaaatga gtacgacctc tgtcgtcgtt gcttattatt tactcttgtc tttatttcca
     2281 ttgattattg cttttggtaa tattttaccg tttctccaca tcgatcaaga gactgtactg
     2341 acctatttac gagaagtgat tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa
     2401 gaccttttga cacaaagttc aggaggcttg ttatccgttt ctgcattagc ggctttatgg
     2461 tctgcaagtc aaagtatcaa tgctttgcaa attgcgatga ataaagcgta tggcgtagaa
     2521 aatagaaaaa attttttgat cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg
     2581 attgctatta gtggggtaac tatcgttctt gggttgggac agctgatttt ggatgctatt
     2641 cagccaatcg tacaaatacc cgtgaacttt atcgatcagt tccaaacttt gaagtggcca
     2701 atcacattgg tagctttatt tgtcatcatg ttcttgattt atttaattgt ccctaatgct
     2761 cagttgaagt tgaagacgat cattccgggc acgatcttcg ctactgtcgg ctggatgctg
     2821 ttatcacaag tgtttagtat ctatgctagg tattttgcaa cgaggatcag cggttatcag
     2881 atcataggaa gttttattgt actcatgctt tggcttaatt ttgctgctac gatcatcatt
     2941 ttgggcggaa tcatcaatgc agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa
     3001 cgtcgagatc caagccgaaa atggttcaat aaattgaaaa ctaaattttc tcataaaaaa
     3061 aagtagaaac aagagttctt attgtaaatt tatatcagta atttataata attatgactc
     3121 ttaattaaag taggtgccca tatgcttgtt tcttttagta tggttataag attttttctg
     3181 acaattttat tatcactgat attaacacct gtattcaaaa tcatttcagt acaaacaggt
     3241 atggtcgaca aaccaaatga aagaagaata aataaagttc caatgccttc agcgggcgga
     3301 ctgcctatct ttatttcatt tgttatatcg actttattct tattccgtaa aattatccct
     3361 caatcgtata ttgtaccgat cttgattgct tctttagtaa tcatagtcac aggagtgctc
     3421 gatgataaat atgagctgac acctaaacaa aaaagtatag ggatcttgat tgcttctctt
     3481 attatttatt ttgtagcaga tatacgtatt gattccttca cgcttccttt tattggatat
     3541 atccaactag gctggcttag ttttcccgta acgatttttt ggatcttcgg tatcacgaat
     3601 gcggtcaatc tgattgacgg tttggatggg ctggcggcag ggatttcatt gatcggttta
     3661 attacgatag gaatcattgg atattttttc ctccatgctt ctacagtgta tattccagtt
     3721 gttattttct gtcttgtagc aagtattatc ggtttttttc cttacaattt ttatccggca
     3781 aagatttatt taggcgatac aggagctcta ttcctagggt ttatgatggc cgtcctttca
     3841 ctacaagggc tgaagaacgt gacttttgtt tcatcgattt ctttgctgat cgtcatggga
     3901 gtgccggtaa cagacacgtt ttttgctatc attaggagaa aagcaaaccg tgtttctttt
     3961 tctacagcag ataaaaaaca tcttcatcac cgactaatca gtttaggctt tactcataaa
     4021 ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt ctttcacagc tatggtcatg
     4081 aattatactg gaagaatcgg aacgatcata ttgattattg ccatgttgtt tgctgcaatt
     4141 ttattatttg aattgattgg tctgatcaat gagaaacatc agtttatttt aaaaacattg
     4201 cgatttttag ggaataaaga gtaccgtacg caagtaaaac agaaatattt gaagaaatgg
     4261 aaccgtcata tagatgaata acaaattaaa ccagccgatc cttattagta ttgtgacggc
     4321 aaacagtgat aaaatttttc ggacactaga taattttatt tcaactgtaa aagatgaaac
     4381 agcagtaaaa atccgaattt ttgacaacaa ttcacttccc gagtacaaaa aaagactaac
     4441 agagtatagg aaatatccct acatcgatat ctattttcat gatgaaaatt ccggatttgg
     4501 ttatggacat aatcacaatc tgatagctag cgattctcgc tatggcatta tttgtaaccc
     4561 agatatttta gtggatgaag agacagttac agcattggtc ttgttattga aagaacatcc
     4621 ggaatgtgcg atgttagcac ctaagattct taatgaagat ggaacaactc agcacctcat
     4681 ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa
     4741 aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga ggattgccta ctgatgctaa
     4801 tagttatgta cggatgatct caggtagttt tatggtagct gatattgata aatttaaaga
     4861 gataaagggg tttgatgagc gtttcttcat gtactttgaa gacaacgact tgtgtttgag
     4921 ttttgaaaaa gcaggttaca agttattgta cacacccttt tatgcagtta tccattttta
     4981 cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa
     5041 atttttcaat aagtgggggt ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt
     5101 gtgctttatc agcaactatt cagtcaatct ccagcttatg gaccattaag caaagcactt
     5161 agcgaaaagc aaattcagct tattatttac gataatagtc ctcaaaggca gcaaaatgag
     5221 ctgtttgaaa aagaaaatat cgtttatcat catgatccgc aaaatcctgg tttagcggtg
     5281 gcttataatt ttgcgttgtc tcaagcagat gaaggaacac gttgtctcgt cactctggat
     5341 caggatacga ggctagtcga cgattatttt gaactattgc gtaaagtaac ctttacagat
     5401 gaatgtgttg ctgcagttcc gatgattttt tctgggagcc gacaagtttc tcccctatat
     5461 tcagatcgtt atatcaatcg tacagctcaa gcagtagaag ttgaaggccc aacagctgaa
     5521 cgaatcatgg caatcaattc aggtgttgcc tggtctgtca aatttctaaa aaagattggc
     5581 gggtttaatc ctgctttttc tttagatttt ttggatcatt ggctgttttg gaaagtggat
     5641 cagttggaaa aaactgtgga gatccttcct gtacgaatgg aacatgatct ttcggtttta
     5701 gactatgaaa aagtttcaag taatcgatac cgttctattt tacaagcaga aaatcgattt
     5761 tatcatgaat atgatcaaag gcatttatcc agtcaccgac gacaattatt gttaagaaca
     5821 gctaaacagt tcttgaccgt caaaaaccgg catatctggc ggatgacttt gcgctcatat
     5881 gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt atagctacct ttaatggagg
     5941 cagatattta agagagcagt tagatagtat tttatctcag ctttcagcag aagatgaagt
     6001 agtcatttct gatgatgggt cagtagatga tacatgggat atattaactg aatacgcaga
     6061 aaaagataag cgaatccagc ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg
     6121 ttatgcgatc cagcagacga aaggagatct aatctttttg gcagaccaag atgatgtttg
     6181 gttggagaca aaagtgaaaa agatcactga ctattttgag cagaatccgg atcagctagt
     6241 agtagtttct gatttaatca ttgtggacag cgagctgcaa gagatccatg attcgtattt
     6301 ttcgtttcga aattccaaag aaggatggct taataatgtg ataagaagtc actatattgg
     6361 tgctggtatg gctttccggt cttctttaaa acccgatatt ttgccgatcc cgtcagaagt
     6421 gccgatgcat gatatgtgga ttggtttgct gggaggaaag aagactgggt tcctaagaga
     6481 accgttaact ctgtaccgaa gacatgattt aaacgcgagt gagattgaaa cacattctaa
     6541 attacggcaa aaaatttctt ggcgtgttca tctgattacg gcgttgatca aacgaaaatg
     6601 gtttaaacgt tgataaaaga tgtttaaatc cttaatattt ccatgacaaa tataacaatt
     6661 gatgagataa taacaaagtg aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg
     6721 aatcattctt gctgggggca gcggaacacg tctgtaccct ttaacaaagg caacatcgaa
     6781 acaattgatg ccgatttatg acaaaccaat gatttattat ccaatgtcta ctctgatgtt
     6841 ggcgggaatc aaagaaattc tgattatttc aacgccacaa gatacaccac gtttcaagga
     6901 attatttgga aacggaaatg atttaggact gcagatcgaa tatgcagtac aagaaagtcc
     6961 agatggattg gcacaagcgt ttatcattgg agaagaattt attggagatg atagtgtctg
     7021 cttggtatta ggggacaata tttactatgg tggcggtctg tctaaaatgt tgcaacgagc
     7081 agcttcaaaa gatacaggcg cgacagtatt tggctatcat gtcaatgatc cagaacgctt
     7141 tggtgtagta gaatttgatg agaatatgca agctttgtcg attgaagaaa agcctgctca
     7201 gccaaaatca aattacgctg taactggcct ttatttttat gacaatgagg taatttctat
     7261 cgctaaaggt atcaaacctt cagaacgtgg agaactagag attacggatg tcaataaagc
     7321 atatttggat aaaggaaaac tatccgtaga acttatggga cgcggatttg cttggcttga
     7381 tacaggaaca catgaatcat tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg
     7441 tcagaactta aaagtggctt gtttggaaga aattgcttac cgtatgggct acatcgataa
     7501 ggaacaattg ctggcccttg cacagccatt gaaaaaaaat caatacggtc aatatttatt
     7561 gaatttggcc gctgaataag gagtccttac agatgaaagt aacagataca aaattaacag
     7621 atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg cggtttcttc acagaaagtt
     7681 attcaaaagc aaaatttgca gaacatggat tgaaatatga ctttgttcaa gacaaccatt
     7741 cactatcagc tgaagctggt gtccttcggg ggcttcattt ccaaaaagga gaagctgcac
     7801 agacaaaatt gattcgggta gtcactggag cagttttaga tgtagtagtg gatatccgca
     7861 aaggtagccc gacttatggt caatgggacg gatttatctt atctgaacat aatcatcgac
     7921 agttgcttgt acctaaagga tatgcccatg gattcgttac tttgacacca aatgtgaact
     7981 tcctttataa gtgtgataat tactataatg cagaagcaga tgggggcatc gcatttgatg
     8041 atccagattt agcgattgat tggccaattg atccatcaag agcgattatg tcggaaaaag
     8101 atcaccgtca tccgacatta aaagaatttg aagcagaaaa tccatttgtt tacggagaaa
     8161 tctaagaatt aaggagaaat tttcttatga aaaacatcat tgttaccgga ggtgccggat
     8221 tcatcggctc taatttcgtt cattacgtgg taaacaatca tccagaagtg catgtcactg
     8281 ttttggataa actgacttat gcaggaaaca aagagaactt ggctggactt cctagcgatc
     8341 gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt agtggaccgt ctagtaaaag
     8401 aagcggacgc agttgttcat tacgcggcag aatcccataa cgataattca ttaaaagatc
     8461 ctttcccatt tgtacaaacg aatatcattg gtacgtatac acttttagaa gcttgccgca
     8521 agtatgatgt acgctatcat cacgtatcta ctgacgaggt ttacggcgat cttccattac
     8581 gtgaagacct gccaggacat ggggaaggcg aaggagaaaa attcactgcg gaaacaccat
     8641 ataatccttc aagtccatat tcttctacaa aagctggttc agatctgtta gtaaaagcat
     8701 gggtgcgttc ttttggctta cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt
     8761 atcagcatat tgaaaaattt attcctcgcc aagtaacgaa tatcttaagc ggtattcgtc
     8821 caaaattata tggcgaagga aagaatgttc gtgactggat ccatacgaat gatcattctt
     8881 cagcagtgtg gctgattttg acgaaaggca gaattggcga aacctatctg atcggtgcag
     8941 acggtgaaga agataataag accgtgatgg aattgatctt agaaatgatg ggtcaaccaa
     9001 aagatgcgta cgaccatgta aacgaccgtg caggacacga cttgcgttat gcaatcgatt
     9061 cgacaaaatt acgggaagag ttaggatgga agcctgagtt tacgaacttc cgtgatgggt
     9121 tggctgacac gatcaaatgg tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg
     9181 ccgttgaagc agcatatgcc aaaaatggac aataatacag agaaaatgtc actgtattaa
     9241 tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga
     9301 gcggataatc atatgttttt attaactgga ggaaatgggc aactaggtac agaattgcgc
     9361 catttattag atgaaaaaaa tctgcaatat gtttcaacag atgcaaaaga cttggatatc
     9421 acagatgccg agaaaacaat ggcttacatc acagaattaa aacctgatgt tattttccat
     9481 tgtgcagctt acacagcagt agataaagca gaagaagaag caaaagaatt agatgagaag
     9541 atcaatgttg atggtacaag aaatgtagca cttgcagcaa aagctgtgaa tgctcaattc
     9601 gtctatatca gtacagacta tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta
     9661 gacgaccaaa cgaatccact aaatgaatat ggtcgtacga aattattagg cgaaaaagca
     9721 gtagaagaga ttttagaaga ctattatatc atacgaactt cttgggtttt cgggatctat
     9781 ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa cccgcgacaa actgactgta
     9841 gtaaatgacc agtttgggcg accaacttgg acacgtactt tggcagaatt tatggtatat
     9901 gtcatcgaag aaaaagcacc atttggtatt tatcatttgt cgaacgaaaa tagctgttct
     9961 tggtatgaat tcgcaaaaga aatattgaaa gatacagatg tagaagttgc acctgttacg
    10021 tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag
    10081 acagaagagt taggatttgt cataccaact tggcaagaag cacttgcgca aatgctggaa
    10141 gctgccaaaa aaatgaataa gtaatttcct tttatcaaat agaatttgga gcatgatatg
    10201 gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa aagcgaacca ggcagattcg
    10261 aatttggtgc ttgacctgtt tctaaaactc cgctatctta ttggcgtttt agtcatcgtg
    10321 gtcatcgtta cttttaattt aaacggaagt tcacttggtg tttgggataa atatgtgtcg
    10381 caaagagacg atgggaaaaa aactgatgtg atttttggac agaatcggga agtgcggtca
    10441 gacgaatggc tggtgcagac acctttttac ctctcacaag ctgaagagga ctttccgttg
    10501 gtgaacgagg attattcatt aaacggacaa aacatgatca ttgcgtataa ttctcctgta
    10561 aaggatatta cggttatcgg aaaaccattc aactggggat tctttttcct cggaagagat
    10621 cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa
    10681 gtactgatga tattaacgaa gagaaaaaaa gctctatcgc taatcggtgc atttgcgctg
    10741 acattttctc cagcagtcca gtggtggttc atgcaacatg tgggcgattt gattttcttt
    10801 actttgggac tgatgactgc tttttatcat tatttctaca atcatgataa aaaatggcta
    10861 cgaacgttga tgatgctttt ggtggttatt ttcggtattg ggtttatttt agtgatttac
    10921 ccagcccatc aagtgatgct ggcgtacttg ctgatttttt attttgttgg cctgcttatt
    10981 tactatagca agaaagtttc atgggatctg ttggatgtag tgttgattgg aggggcagtt
    11041 ttatttattg gcggagtcat ggttcatttc tggctaacgt caaaagacgc attgttagct
    11101 tctttgaata ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg gacgattggc
    11161 gaattttttt ctttccttac aaattggaaa attccttttg aagatatcaa ctattcgaat
    11221 aactctgaag tggccctgtt ctatcacttt tttccaactg tattcctagc aagtccattt
    11281 gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt gatgattttt
    11341 tgtctgtttt ctatcttttg gattaccgtt gggttgccaa aatggctagc agaaatcact
    11401 ctgctgtctt atgttcctcc attaagagcc atccttactt ttagttttgc ggcttgttta
    11461 ttatcgatct ggtttatcgc ttatatttgg agagaagatg ttattcctat ttggtataaa
    11521 ttcattttgt tggcagtaaa tgcatggtct tatttctatg cgattacgca ttcgaaaatg
    11581 gataattatt tttcggattt tgaaaccatc gcagttgctg tgttgggaac agtgatttta
    11641 gcttttgcat tatttggctg gagacgcttg ttttacctcg cttttgccgc cttagttatc
    11701 gtatctggct ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca
    11761 ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa
    11821 gatgaattat ataacttcac cccaacattg ggagtgaaat ccttcaatac agtacgcttt
    11881 tatccagata tagctgcatg gaagaaaatc gatccgaaag aagaatacga gaagtattat
    11941 aatcggtatg ctcatacaag agcttttatc gctaaggaag atacaaagtt cattttagat
    12001 cgtccagata tgttttcggt cacgttgaat tttgaagacg cagaaaaatt agggatcgac
    12061 tatgtgatca gtaaacgacc attaactgat tacaaccaat tatatgaagc gcagttcacg
    12121 cagctatatg gaccggataa agacggttat atgattttta aagtagaata tccggattat
    12181 ccagatgtac cacaaacaca aactcaggaa aatcagctag tcgtacagta ataggctagc
    12241 tgagaataat cctgaaatgt tagtagaaag gagagtggag ataattcaag caatcgtaca
    12301 aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg ttactgacgc tagtcttttt
    12361 tgtttggaca gcagggttag gttttccagc aactttgttt atatataaca atagttcatt
    12421 acttatcgct tttagtgtgg ctattttgct cattttaaat gtttataaag caaaaggcgt
    12481 cgattttctt tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt actttcggga
    12541 attaagaaat gagtctttat ttggagactt actgtttcct ttgattattt tattttgttt
    12601 agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt atttatctga ttgtcttttc
    12661 ggtcttcctc ggaatcacgt ttatcgtgta tttacagaga tccaagtacc agaaggacaa
    12721 agcatctggg ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga
    12781 agttctttga tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg
    12841 tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt ttctcgggat ttgggtctgc
    12901 caatctagag gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa
    12961 tcgttgatgc gtgtcattcg tgcgccattt attgcttaca cggttacgat tcttttagct
    13021 ttgccgattt cttatctggc ggctgtctct gaaaaagtca atttgtttac cggtcgagaa
    13081 gatatttggc ataaattcta tcagacgcta ggagaaaaat ctgaacaaat tttactagga
    13141 atgaaaacat ttatcttcca acgaggaaac caatttttag gaaaccacaa tagttataat
    13201 tctattttga atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt
    13261 tttattggtc gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg
    13321 gccttttttg cggtgatgat gcagtcattt atggaggata cattgacttc ataaccatgg
    13381 gttccgatta tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt
    13441 gcggaggaat tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct
    13501 tatgaggaac aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca
    13561 caaaatacga gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct
    13621 agcatcttct gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag
    13681 aaataagaac gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact
    13741 gaaaatcgct tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt
    13801 atcagaacag aaaattattg ctacagtttt cgatcaatga cttcaaatca agatatgcag
    13861 gatcgttttt aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt
    13921 ggctggtatt tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg
    13981 tatatttagt taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta
    14041 cggttgtttt tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct
    14101 tgcccacgac gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca
    14161 tagctgtcgc gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt
    14221 atttattttg catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac
    14281 catttttacc tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat
    14341 taccgatatt atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa
    14401 atccgctgta ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct
    14461 gggagcatgg aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg
    14521 gctctgcagt gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc
    14581 tatgacagaa tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa
    14641 accttcggat cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt
    14701 ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg
    14761 agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac
    14821 atccggcacg atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt
    14881 caatcctgag tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc
    14941 aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggagacca
    15001 tatttatcaa cctgtaaaaa cttattcaag cgggatgttc gttcgtctag cttttgctgt
    15061 agcaatcaat gtgaatcccg atattttgat tgtggatgaa gcattggcag taggcgatct
    15121 tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat
    15181 tttattcgtt tctcatgata tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt
    15241 gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa gtaacgacga attatgaaaa
    15301 ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt
    15361 cgccgctcca gatatcgtcg acgtgacgaa agcggaattg ttggataaag acaaaaatcc
    15421 ggtggatatg ctggaacaag gagaaaaact atacgttaaa gtaacttatg aagtaaaaga
    15481 tgatacaatc aaaaatccgg ttttaggaat tgcgctacga acagtcgaca atttttatgt
    15541 atgcggactt aatactctac tagatggtat caagattcct tggaaaaaag gagagaatat
    15601 cttttatctg gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc
    15661 ggtctttgaa gaaaatgcaa cggttccttt ggtatataag acaaagtaca tgaacttgtt
    15721 tgtcagcggt tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg
    15781 gactcatagc aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa
    15841 aaattgccgc acagatcaaa ccaggaagta aagttttaga gtttggtccc ggaaacggcc
    15901 gattaacgaa acatttaatc ggagcaaaac aatgtgaagt gagcattgtt gagttagata
    15961 aggaattgtt cgattttgtc agcgaatttg ctcaagatgg attttatggt gatatcgaaa
    16021 gttttgagtg ggcaaactat tatgctggac agacatttga ttacgtcttg tttgcggatg
    16081 ttttagaaca tttggtagat cctggaaaaa cattgaagaa agtcagagaa tttttgaatg
    16141 aagaaggaga gatcctgatt acattcccta acttggcgca taattctgtc atgatcgatt
    16201 tgttcaataa ccaattgccg tgggcttctt atggtctcct agatgaaacc cataattctt
    16261 tttatactca tgatggcttc caaaaagttt ttgaaaaagc gggattattc attaatattg
    16321 aagattattt gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc
    16381 cggaagctgt acgctatgat ttcaaaatgc gaccatttgg tgaagtctac caatactttt
    16441 tctcattgat gaaacatcca gtagcacaaa gttccattgc agagccacaa aattctaatt
    16501 atgtaaaagt gttagaagta acccagcaaa cgaaacaaga tgaaacaatc caacaaatac
    16561 cattcaataa tttcacaggg gaaaatgaga cactaagttt ccccgtatca gaaacaacag
    16621 aaagaatggt gtttcgcttc gcgcaacaac ctagctttat cgaattcagc gcagaagctg
    16681 ccggagaaaa actaacattt atcgattcga atgctgtagt caaaacagta aatgattgtt
    16741 atttgtttga tgggaaagaa ttaccagagt tcgttctaac ggatatttct gggaaagaag
    16801 tgactatcca ttgtcactat cgctttatcg gggaattgac accaacaatg aaagaattgt
    16861 tggaaacgat tcgtcccatg gctgaagtca caaagcagct ttctgagaaa aatgacgaat
    16921 tagaaagaga aaatcatcat cttcttgagg aaaatacaag actagaccat acgttaaaaa
    16981 caaccacaaa tcgctattgc acattgttgg aaagtgatga atggacaata aaacatagac
    17041 taggacgacg taagaaagaa acttcgaaaa aaatccaaga aaaagaactt tctatatgta
    17101 ttgacgagaa aatctgggat gcagaaacaa aaatattaaa aatcatcgga tggggaatcg
    17161 ctaattcgga tcgccagccc ttgtcctata agttatcagc tgaccaatcg ccatttttcg
    17221 aagcaattcc tgtttcacga gaagaagtca atcaagcaga acagctcgca aaaggaacag
    17281 aagcgggatt tgagttacga atcctttgcg agcaagaacg ttcattctta gtggaagcag
    17341 tagctcaaaa tggccaatca tggatcattg aaatgtaaat aatgattaac ttataaagat
    17401 aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg
    17461 tgaacgggcc actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga
    17521 aatccctacg ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag
    17581 attggatatc aatcatttat ataatcttga tgttaagacc caaagcggtt ttaaggtacg
    17641 tttatcaggg aaaatgagag gaaaagccat cttagacttc caaacagcga gacatcaaaa
    17701 tggcatagct gtgaaattaa acggtaaata cccttatgat gacggcattg aatcttcatg
    17761 ggaaagaaaa aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa
    17821 aaaagcgatc cgtcgggtca aattggaatt gaagccagga tcgatcgact atgcagagtg
    17881 gatcagtcgt catgagaaac cagatttgaa agagcaaagg aaagaagcac aaggatttgc
    17941 gtaccgtcca ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga
    18001 aaaatgcatt gattcggtac tggatcaaac gtatgatcat tgggaactat gtatcagtga
    18061 tgatgcttct actgatccag ctatccgaaa gtgtctggaa tcctatcaag caaaagatga
    18121 tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc agtctggcaa ctaattctgc
    18181 attggaaata gcagaaggag aatttatagc actcttagat aatgatgatg aattaccgcc
    18241 atttgcatta tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atgtgatcta
    18301 cagtgacgaa gataaaatcg atgcagatgg caatcgcttt gatccgcatt tcaaagcaga
    18361 ctggtcgcct gatacattga tgggaaataa ttatatctct cacttaggcg tatatcgaac
    18421 aagcattgtt aaagaacttg gcggtttccg taaaggctat gaaggttctc aagattatga
    18481 tttggtcctt cgtgtgacag aacaaatccc tgcagatcat atttaccaca ttgaccgagt
    18541 cttgtatcat tggcggacga tcccaggatc aactgcaagt aatggcgaag ccaaaagtta
    18601 tatctatgat tccggagtga aagcattgac agatgccctg tctagaagaa acatcaaagg
    18661 aagcgtacgt cctggcagaa tttctggatt ttatgagatt gcttatgacg tcttgcaaga
    18721 ggatttggtc agtgtgatca ttccaacgaa gaatggctat gaagatctaa aaacttgtgt
    18781 ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa atcattattg cggataacgg
    18841 aagtacagat ccaaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg
    18901 ctttatcgta gaattgattg acattccatt caattattct cgaattaaca atttagcagc
    18961 agaaaaagca aacggcaaat actttttatt cttgaataac gatacagaag tgattgagcc
    19021 agattggatg acggtgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc
    19081 aaaactgttt tatccagatg atacaacgca gcatgcagga gtattattag gaatcggtgg
    19141 tgttgcaggg catgcattga ataactatga tcgaacacat tgtggttatt tcggccgatt
    19201 ggtcattgac gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga
    19261 cttcaatgct gttaacgggt ttgatgaaac attagaagta gcattcaacg atgttgactt
    19321 gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta
    19381 ccatttcgaa tctaaatcaa gaggttatga agatactcca gaaaaacaaa gacgttttgc
    19441 tggtgaaatc aaaaaaatgc aagataaatg gccagcatat attgcgcatg atccatttta
    19501 caatgataat ttaacaaaag aaggaatcgg cgacttttcg ttgagaccag attaaactat
    19561 aataaaaagc tgttatgttg tgattcgctc gcacataacg gctttttata gttttttgtc
    19621 aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga ggttgggaca gaagt
//
LOCUS       DACEND010000044        19464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEND010000044 DACEND010000000
VERSION     DACEND010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.045"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW746_001306"
     CDS             127..3654
                     /locus_tag="IW746_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1078214.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW746_001307"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW746_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1078215.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW746_001308"
     CDS             7442..7783
                     /locus_tag="IW746_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1078216.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW746_001309"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW746_001309"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1078217.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW746_001310"
     CDS             9140..11560
                     /locus_tag="IW746_001310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1078218.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW746_001311"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW746_001311"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1078219.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW746_001312"
     CDS             12994..13731
                     /locus_tag="IW746_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078220.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW746_001313"
     CDS             13745..14560
                     /locus_tag="IW746_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1078221.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW746_001314"
     CDS             14573..15220
                     /locus_tag="IW746_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1078222.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW746_001315"
     CDS             15236..15892
                     /locus_tag="IW746_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1078223.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW746_001316"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW746_001316"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1078224.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW746_001317"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW746_001317"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1078225.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW746_001318"
     CDS             18234..18752
                     /locus_tag="IW746_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1078226.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW746_001319"
     CDS             18859..19359
                     /locus_tag="IW746_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1078227.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6545 a         3344 c         4179 g         5396 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caag
//
LOCUS       DACEND010000045        18720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEND010000045 DACEND010000000
VERSION     DACEND010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.046"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..1076
                     /locus_tag="IW746_001320"
     CDS             180..1076
                     /locus_tag="IW746_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1078228.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1226..2167)
                     /locus_tag="IW746_001321"
     CDS             complement(1226..2167)
                     /locus_tag="IW746_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1078229.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2293..3414
                     /locus_tag="IW746_001322"
     CDS             2293..3414
                     /locus_tag="IW746_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1078230.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3672..5174
                     /locus_tag="IW746_001323"
     CDS             3672..5174
                     /locus_tag="IW746_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1078231.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5241..5768
                     /locus_tag="IW746_001324"
     CDS             5241..5768
                     /locus_tag="IW746_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1078232.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5979..7040
                     /locus_tag="IW746_001325"
     CDS             5979..7040
                     /locus_tag="IW746_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1078233.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7043..7939
                     /gene="murQ"
                     /locus_tag="IW746_001326"
     CDS             7043..7939
                     /gene="murQ"
                     /locus_tag="IW746_001326"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1078234.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7953..9935
                     /locus_tag="IW746_001327"
     CDS             7953..9935
                     /locus_tag="IW746_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1078235.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="IW746_001328"
     CDS             complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="IW746_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1078236.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10657..12081
                     /locus_tag="IW746_001329"
     CDS             10657..12081
                     /locus_tag="IW746_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078237.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12262..13035)
                     /locus_tag="IW746_001330"
     CDS             complement(12262..13035)
                     /locus_tag="IW746_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1078238.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13032..13937)
                     /locus_tag="IW746_001331"
     CDS             complement(13032..13937)
                     /locus_tag="IW746_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078239.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14091..14411)
                     /locus_tag="IW746_001332"
     CDS             complement(14091..14411)
                     /locus_tag="IW746_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1078240.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14885..15571
                     /locus_tag="IW746_001333"
     CDS             14885..15571
                     /locus_tag="IW746_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1078241.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15573..16517
                     /locus_tag="IW746_001334"
     CDS             15573..16517
                     /locus_tag="IW746_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1078242.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16716..17360
                     /gene="pcp"
                     /locus_tag="IW746_001335"
     CDS             16716..17360
                     /gene="pcp"
                     /locus_tag="IW746_001335"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1078243.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17439..18716
                     /locus_tag="IW746_001336"
     CDS             17439..18716
                     /locus_tag="IW746_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078244.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6004 a         3304 c         3980 g         5432 t
ORIGIN      
        1 agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt
       61 catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct
      121 ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga
      181 tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca
      241 tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag
      301 ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg
      361 agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc
      421 acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg
      481 agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat
      541 tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg
      601 caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag
      661 atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta
      721 cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg
      781 agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag
      841 tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc
      901 ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc
      961 tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg
     1021 attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa
     1081 cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga
     1141 gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt
     1201 cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga
     1261 ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc
     1321 aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt
     1381 acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt
     1441 acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata
     1501 cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat
     1561 ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat
     1621 aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt
     1681 tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta
     1741 acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga
     1801 tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac
     1861 acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt
     1921 gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt
     1981 ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc
     2041 catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc
     2101 cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga
     2161 ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga
     2221 agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag
     2281 tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca
     2341 tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat
     2401 gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga
     2461 acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta
     2521 ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta
     2581 gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga
     2641 gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga
     2701 agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg
     2761 tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt
     2821 cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa
     2881 caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc
     2941 ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta
     3001 gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga
     3061 gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc
     3121 gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga
     3181 aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt
     3241 tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt
     3301 attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa
     3361 acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca
     3421 aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca
     3481 ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga
     3541 agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg
     3601 tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga
     3661 ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag
     3721 gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta
     3781 tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt
     3841 atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata
     3901 aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg
     3961 aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac
     4021 tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt
     4081 tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg
     4141 cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg
     4201 atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga
     4261 atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg
     4321 aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt
     4381 ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta
     4441 gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag
     4501 caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg
     4561 aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg
     4621 tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg
     4681 agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc
     4741 attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc
     4801 tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg
     4861 cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg
     4921 aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg
     4981 aattggctaa atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt
     5041 atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca
     5101 tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc
     5161 ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg
     5221 ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg
     5281 cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg
     5341 cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt
     5401 cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc
     5461 tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca
     5521 aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt
     5581 cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag
     5641 aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc
     5701 atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt
     5761 atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta
     5821 gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat
     5881 gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat atactctagt
     5941 catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta
     6001 tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct
     6061 ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc
     6121 gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt
     6181 caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa
     6241 ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt
     6301 cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat
     6361 tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc
     6421 ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag
     6481 gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag
     6541 gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat
     6601 tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg
     6661 agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg
     6721 aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg
     6781 tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag
     6841 ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg
     6901 agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag
     6961 ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta
     7021 taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg
     7081 cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat
     7141 gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc
     7201 agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc
     7261 cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt
     7321 agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt
     7381 cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac
     7441 aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg
     7501 attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc
     7561 cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac
     7621 aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac
     7681 tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac
     7741 aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta
     7801 cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat
     7861 gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt
     7921 tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag
     7981 aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc
     8041 actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat
     8101 taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg
     8161 gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag
     8221 gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc
     8281 gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta
     8341 aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt cctgcatttg
     8401 tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc acagccggaa
     8461 cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga
     8521 tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacac
     8581 caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat ccggaagcac
     8641 cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg
     8701 tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg
     8761 atgcggtaga tatcatcgtt acaccaactc tttcgttgat tgccatcggt ctgattgaaa
     8821 ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt gggatcaact
     8881 gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta ttcttgccga
     8941 tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg attgatgaaa
     9001 ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag
     9061 cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag
     9121 ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta acattacctc
     9181 taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca gtgatcggaa
     9241 tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg atcccattga
     9301 tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat gctggtggct
     9361 tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa gaattagcga
     9421 ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa
     9481 gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg
     9541 atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca gaaaacggac
     9601 atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa cacgctattg
     9661 ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac acagttgact
     9721 taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc
     9781 ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca tccatgatcg
     9841 tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa
     9901 aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat agaaacgaaa
     9961 gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt tttctgctaa
    10021 aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat ctgccaagaa
    10081 aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat tattgaagtt
    10141 tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat gttccagata
    10201 ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat ttccgccgtc
    10261 taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat cactattttc
    10321 atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca ttttttcagc
    10381 atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata tatataaaat
    10441 gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta agttttttgg
    10501 tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa agccggtaga
    10561 attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt tgttatactt
    10621 tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa
    10681 ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta
    10741 tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta
    10801 ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt
    10861 ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt
    10921 tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca
    10981 ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca
    11041 tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc
    11101 gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta
    11161 tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc
    11221 gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc
    11281 atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga
    11341 gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt
    11401 atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg
    11461 atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca
    11521 gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc
    11581 ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga
    11641 aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat
    11701 gtctttttac tttcattatt attcactgac agtgcttata actttgctta cacacttggt
    11761 acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat
    11821 cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg
    11881 gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc
    11941 atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta
    12001 gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc
    12061 actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc
    12121 gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt
    12181 tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac
    12241 agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc
    12301 taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt
    12361 caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg
    12421 attccatttg atccactccc atttttggat gagtgcaata aggataccag atatgattga
    12481 cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca
    12541 agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata
    12601 agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga
    12661 agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac
    12721 agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg
    12781 ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc
    12841 tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac
    12901 tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac
    12961 taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt
    13021 tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa
    13081 gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat
    13141 agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga
    13201 tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta
    13261 gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg
    13321 atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag
    13381 tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca
    13441 tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga
    13501 tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc
    13561 gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat
    13621 acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata
    13681 cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc
    13741 tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc
    13801 atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt gccaggggat
    13861 aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg
    13921 accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg
    13981 atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga
    14041 atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg
    14101 ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa
    14161 agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc
    14221 cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag
    14281 taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca
    14341 tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc
    14401 atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc
    14461 atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta
    14521 tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta
    14581 aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat
    14641 ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact agaccttatt
    14701 tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag taagataatt
    14761 tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt gagaaaacaa
    14821 ctatattttt tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa
    14881 atatatggaa aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt
    14941 taaattagac tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg
    15001 tcttggcatt caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat
    15061 ggcgattttt atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga
    15121 atcagcggct tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat
    15181 ttatgggatc tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg
    15241 atttgttcgt ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga
    15301 agtccatcct aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg
    15361 ctggttcttt ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac
    15421 actgaaagat ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt
    15481 ggctatcacg gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc
    15541 gaagaaatat tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt
    15601 tattaggcga taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca
    15661 ctggagtaag aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct
    15721 ggacggtctt aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg
    15781 gcttgatctt tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc
    15841 ggcttccatc aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta
    15901 ttccagcttt gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg
    15961 ctttagtcgg cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc
    16021 gagataatcg accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc
    16081 cgttaagcat gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag
    16141 ttgggacagt gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg
    16201 gcatcatcat ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg
    16261 cggccatcac cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga
    16321 atccagcgtt aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac
    16381 cgatggctgc taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg
    16441 gagtgatcaa aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata
    16501 tgattctatt taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc
    16561 taacattgta gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt
    16621 atttaaacta ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa
    16681 ccatatgtta aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg
    16741 atccatttgg tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg
    16801 aaatcagcgg tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc
    16861 aagtagtcaa agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag
    16921 caggtgggcg gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa
    16981 tccctgataa tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg
    17041 cttactttac ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac
    17101 cagcgtacat ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc
    17161 ttcatttgat cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct
    17221 cacccgatca agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg
    17281 cttcttcttt gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa
    17341 atgccggaac aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag
    17401 tagccagctt tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct
    17461 ttgaagaatg gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat
    17521 acacaagcag tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag
    17581 tcgataagca ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg
    17641 caacagattg ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc
    17701 agcaaaaatt gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc
    17761 caaaaaccgg aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct
    17821 ctccgacagt ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg
    17881 aattattatg gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact
    17941 gttttgtctt gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag
    18001 ctcagcggct cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag
    18061 cattggaaaa tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac
    18121 agataaaaaa agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat
    18181 tcttgatcaa ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag
    18241 tgccagtaca tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac
    18301 gtcaacgaac accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca
    18361 tccaaccaaa aatctatcta tttatttgga agcaatattg tcactatggt tttacagaag
    18421 ggttcttcga cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag
    18481 aacgagaagc tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc
    18541 aactagtagt gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag
    18601 gaaaacatta tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag
    18661 aagaatcact gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa
//
LOCUS       DACEND010000046        18559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEND010000046 DACEND010000000
VERSION     DACEND010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.047"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..160)
                     /locus_tag="IW746_001337"
     CDS             complement(<1..160)
                     /locus_tag="IW746_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1078245.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            426..770
                     /locus_tag="IW746_001338"
     CDS             426..770
                     /locus_tag="IW746_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1078246.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            771..1190
                     /locus_tag="IW746_001339"
     CDS             771..1190
                     /locus_tag="IW746_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1078247.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1282..1632)
                     /locus_tag="IW746_001340"
     CDS             complement(1282..1632)
                     /locus_tag="IW746_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078248.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1598..2929)
                     /locus_tag="IW746_001341"
     CDS             complement(1598..2929)
                     /locus_tag="IW746_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1078249.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3185..3751
                     /locus_tag="IW746_001342"
     CDS             3185..3751
                     /locus_tag="IW746_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078250.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3896..3983
                     /locus_tag="IW746_001343"
     tRNA            3896..3983
                     /locus_tag="IW746_001343"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3928..3930,aa:Ser,seq:cga)
     gene            4317..5291
                     /locus_tag="IW746_001344"
     CDS             4317..5291
                     /locus_tag="IW746_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1078251.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5616..7511
                     /gene="asnB"
                     /locus_tag="IW746_001345"
     CDS             5616..7511
                     /gene="asnB"
                     /locus_tag="IW746_001345"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1078252.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7706..8152)
                     /locus_tag="IW746_001346"
     CDS             complement(7706..8152)
                     /locus_tag="IW746_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1078253.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8380..8529
                     /gene="rpmG"
                     /locus_tag="IW746_001347"
     CDS             8380..8529
                     /gene="rpmG"
                     /locus_tag="IW746_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1078254.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8617..9135
                     /locus_tag="IW746_001348"
     CDS             8617..9135
                     /locus_tag="IW746_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1078255.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9212..10168)
                     /locus_tag="IW746_001349"
     CDS             complement(9212..10168)
                     /locus_tag="IW746_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1078256.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10334..11140
                     /locus_tag="IW746_001350"
     CDS             10334..11140
                     /locus_tag="IW746_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078257.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11137..12126
                     /locus_tag="IW746_001351"
     CDS             11137..12126
                     /locus_tag="IW746_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1078258.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12123..13127
                     /locus_tag="IW746_001352"
     CDS             12123..13127
                     /locus_tag="IW746_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1078259.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13259..13801
                     /locus_tag="IW746_001353"
     CDS             13259..13801
                     /locus_tag="IW746_001353"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1078260.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13821..14519
                     /locus_tag="IW746_001354"
     CDS             13821..14519
                     /locus_tag="IW746_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1078261.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14540..14752
                     /locus_tag="IW746_001355"
     CDS             14540..14752
                     /locus_tag="IW746_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1078262.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14752..15714
                     /locus_tag="IW746_001356"
     CDS             14752..15714
                     /locus_tag="IW746_001356"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1078263.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15732..16127
                     /locus_tag="IW746_001357"
     CDS             15732..16127
                     /locus_tag="IW746_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1078264.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16290..16655
                     /locus_tag="IW746_001358"
     CDS             16290..16655
                     /locus_tag="IW746_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1078265.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16652..18463
                     /locus_tag="IW746_001359"
     CDS             16652..18463
                     /locus_tag="IW746_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1078266.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
BASE COUNT         5962 a         3131 c         3834 g         5632 t
ORIGIN      
        1 attctaaggc tgattttcct tgcttgaaga acagatatac tttatggatc gtttgtcttg
       61 aacgattcaa gcaatgggca gttttagcaa cagattgatt cattttgaaa taagactcta
      121 ttattacaag ttcatccgtt gtaagatggg tataggtcat ttgtactcac tccttataat
      181 tctttggtcg gaactatttt gagtgtagca caaatgactt ttttagttgt ctagcttaat
      241 tttacaatcg gcgaataaaa aaagcgttag caactatgct taaaaagcat atcgactgaa
      301 taaactaagc attggacagg ctgaaagact acatttaaaa tgaaatgcga agaaacaata
      361 gaaaataaag ttgccttaga accatagttg aatcacctaa cagtcgtgaa gggagaataa
      421 gagaaatggc agaaaaacaa accgcaggac gagatcaatt aggagatttt gcaccaaaat
      481 ttgcagaatt gaatgatgat gttttatttg gacaagtatg gtccagagaa gaattatctg
      541 ctcatcagcg tagtatcatc accatatctg cattaatttc aggaggaaat tttgaacaat
      601 tgccagctca tctgaagatt ggcaagcaaa atggcattac tagtgaagaa atagcagaaa
      661 tcattaccca tttagctttt tatgtaggat ggccaaaagc ttggtcagct tttagtttag
      721 caaaagaaat ctatttagaa gaaaatagcg aagataagaa agaggcgtaa atgacgacaa
      781 aacacgaaga aatacacaat ggcgtgattt ttccagcagg tgaaaaaaac gaagcttacg
      841 ctcaagtatt taccgggcaa agctatctga atatgttagt gaatgatccc gaagtttctg
      901 ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa tcactggcat gtccataaag
      961 acggctatca aatcttactt gtgaccggag gagaaggctg gtatcaggaa gaaggaaagc
     1021 cagcaagacg attaatggct ggagatgtcg tcgtaacgca caaagggatt aaacattggc
     1081 atggtgcgac aaaagacagt tggtttgaac atttggcgat cactgctggt aattcagagt
     1141 ttttagaacc tgtaacagat gaagtgtatg aaggattgga aaatcaataa tgtcaagctt
     1201 gaacagctcc tgaaagtaga ttttaatcga actttcagga gctgcttttt tgattcagtc
     1261 ttttataaat agaaaatagg gttaataata ggaatgagaa gcacgtcgaa agatcttaat
     1321 attgataatc ggctgggcat acatgatttc cacgtcatca atcaaactac ctaacagatg
     1381 ataatcttcc cgatcatgtc ggggattacc taatagttct tgataataga tttccatact
     1441 agggtcccga ggattgttca gcaaatcagc aagtacatct aaatcttctg gctgagtttc
     1501 tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca aattctactc caatacgtat
     1561 ttcagttatt tcatgggaat ataaataagt aagaagctca tcgatgattc tttttgataa
     1621 ttctttttcc aagtgtcttt ttttcctttc tgacagcggt aaagttatct agtatcggta
     1681 caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt atatagattt agtcctccgt
     1741 gcaaagtgct gacgttatgg acaagagcaa tatgtagtac accagctatt acaccgtaaa
     1801 aactgccgta aaaaccgctt atgggcgcga gacctgtccc aaacacagct gcaacaagca
     1861 tgccagtact ggtgatctca tgaaaagttc caaacaaaga agcaataaat attccgacta
     1921 gtatcggaaa actgtttttt aaatgacaac caaaagcact gaaacccacc gcagataaaa
     1981 tagcaccgat gacaggtcca tttaactgac cgccaaccag caaaacataa gaagacagca
     2041 tcagaccaac taagcctatg ttgatcaaag tagcaccaat accagaatta gcaatgaaat
     2101 cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat ccccaaaagc cgaaaggaat
     2161 tataatagaa accaattccg actgtcagcg aaaaaaagcc aaaaataatc caaatgagag
     2221 gaaagtgata ttcattgaaa accaatgtat tttcgacgat tgaatagctg aataacctca
     2281 aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa tccaatatta taaagtgtga
     2341 atccacgatg gaaaactaaa gtttgagcag ctaagggcgg aagaagtaga ccaattgcta
     2401 tgccagctaa attccccaat aaaataccta tcagtaaagg aaaacgcata ccaaaagtga
     2461 tataactaac gaccggtgat aatgcgctac caaacagtcc gatcatgatg tactgagaaa
     2521 aaggtttatt gacaaataca gcgtatagat acacacctaa aatgattgag atagaattat
     2581 aaaggttttt gccaaaaaaa gagaaaccgg taactgttag aatcgaagca aataagggac
     2641 cgtttaaaat caccttgtgc cgataagcta aaagcacaga aagtaaggtc aaaatcccta
     2701 catttatgaa tgcgcttccg aaaccgccta aagcaaaata atctgtcaaa agattgcttg
     2761 gcgaggtaag aatagttagc aatccactcc aattatcagt aggagtatta gaaagcaagc
     2821 caataaaaag aaaaaaaaca cctgtaaaac caagataaaa atactttctt ttctgtgaaa
     2881 cgattcgttt ttgaggatga atataaataa cgtcagtttc gttttccatt acacaccttc
     2941 tttttcattt tctttaacta taacttgaaa tgaaagattt ggacaactat catttaggtc
     3001 aaaactaaaa atgtaaggga acccattgaa tttaaccaaa tttttttaat tttgcaaaaa
     3061 aaagttaaag aagactgaaa aaaaatttaa aatataacgt tttgagtaaa atgtttgcaa
     3121 aaaatgctaa aaatagggta caataaaaca acatatactg cagtaaagaa gtgggggagt
     3181 tttaatgaga agaaaagtct ataccaagga tcaaattcta aaagctgctt atgatgttgt
     3241 tgcaaaggaa gggtttaaag gatttactgc gagaaatatt gctaaaaaga tggggatttc
     3301 gacacagccg atttatctgg aattcaaaaa catggaagat ttaaaaaata cgttgctgga
     3361 ttctatcttt gaacaactaa aagaagatgt tttccctatt gaacgtactg gtgataaaat
     3421 cattgatctc ggtttgaact atatctactt tgcaaaaaag catcatagtc tttacatggc
     3481 attgtacatt gatgaacatg gggacggaaa gaaaatgcat gatttttcat acaattattt
     3541 ccgtgaaata gttaaagaag atgaaagata caatcaatta tcggatgaat atgtcgatgc
     3601 tctgcatact ggtacatggg tagcagtgac tggcttagcg tcattgatgt catcaaacat
     3661 catgcatcca acagaagaac aaatcattgc actgatcaaa cggacaattg actcgatttt
     3721 agcattagat aatcctgaaa taatcagata gtgttctatc agtatgaatt tgatcggtaa
     3781 gtccgttggc tgcaaactgt caatggactt ttttgctaaa aaaagcgatt tctgttattt
     3841 atctattaga aataggcttg acatttcgtg aaaaaagttt aaaaatagta cttacggaag
     3901 gttggcagag tggtaatgca ccggactcga aatccggcga accggctaat accggcgcgc
     3961 aggttcaaat cctgtacctt ccttatatca acgattagag gttgtgaaga agctgtcctg
     4021 catcaagtaa taagaacggc caaacaaaaa tagcttttcc tattttttcg tttgattggg
     4081 cttattaccg cagatgcaag cttttgaaca ccgtttgttc ggaataaggg gctgtgacaa
     4141 gacttttgtc acagcccctt attgttgtac ttttaaccta caatattttt tgattccccc
     4201 tgagtactat tttttataaa tgctagtaaa tcaatcttct ttggcctttt tttgataaaa
     4261 aaaatactag atgtaagata aaagtgtaaa aacgatttgt tttaggacgg gaaacgatgt
     4321 gtaccgcaat cacttatgca acgaaagacc attattttgg aaggaatttt gattatgaga
     4381 tgtcttataa tgaagtggtc actattactc caagaaatta tcgtttcgat tttagaaagg
     4441 taaaaaattt agataaacat tatgcaatga taggaatagc agctggggta tccaactatc
     4501 cactctatta tgaggctaca aatgaaaaag ggttgagtat ggcaggactg aattttcctg
     4561 gcaatgctga ttataaagaa ttacaagaag gaaaagataa cgttgcacca tttgagttta
     4621 ttccatggat actagggcaa tgctcaacca tagacgaagc taaagaatta ttggctacta
     4681 tcaatctagt caatatcgat tttagtgaaa aactaccctt gtcaccttta cactggttat
     4741 tagctgataa agaaaaatcg atagtaattg aaagtatgaa agatggactt catttatatg
     4801 ataatcctgt cggtgtgttg acaaataatc cgccatttga ttatcaatta tttaatctga
     4861 acaattatcg ctcgctatca aatggaacac cagaaaatca tttttcaaac cagatcagct
     4921 tgaatgttta tagtcgtggg atgggaggat tgggcttacc tggagatctc tcttctgttt
     4981 ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc atcaggtgat tcagaatctg
     5041 aaagtatcag tcaatttttt catatactag gctcagtcga gcaacaaaaa ggagtatgcg
     5101 atactggaga aagaaaatac gaatatacga tttattcttc ttgctgtaac gttgataaag
     5161 ggatttatta ttatcgtacg tatgaagaca gtcaaattac tgctgtaaat atgaataaag
     5221 aagacttgga tcgtcatgaa ttggtcagtt atccagtcat aaaagagcaa cagataaact
     5281 atgtcaatta attaatgaat agatccgttt ggtatcatat tttctgatac agtcataatt
     5341 agacacgatc tttaggtcgt gtcttttttt gctggttttt ttggaaatag catccactaa
     5401 tattttttta acacgaagag aataaggtag cctcaaaaaa gaaaacatgg taaattagat
     5461 attgggactt tattttctgt aaaaacagaa actttcatct tttgttttca cgtgacgaaa
     5521 ttgtaagaga gaaaatattc gtccgctatt tattctatga taagatgcat atgaatgaaa
     5581 taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg cggtattgta ggttttgtta
     5641 acgacaagga caacaaaaaa acaattatta atacaatgat ggatcgaatc gttcaccgtg
     5701 gacctaatag ttcaggagaa tatatcgata aacatgttgc tttaggattc agaagattaa
     5761 gtattattga cttagagggc ggtactcagc ctatttacaa tgaagatcgg acaaagatca
     5821 ttatttttaa tggcgaaatc tataattacc agcctttgag agaagaattg attgcagctg
     5881 gccacgtttt tcaaacacat gcagatacag aagtcctttt gcatggttac gaagaatggg
     5941 gaacagaatt attgcaaaag atccgcggaa tgtttgcttt tgctatttgg gataatgaaa
     6001 agaatgagtt attcggtgca agagaccact ttgggatcaa accatattat tatgcagaaa
     6061 tgaacggaac atttatgttt ggttcagaaa tcaaaagttt tctgcctcat cctgatttta
     6121 acaaagaatt gaacaaagaa gcgttgaaac cttatatgac tttccaatat tctccattaa
     6181 acggcgaaac attcttcaaa gatgtctatc gtttgcctga aggccattat tatacgtata
     6241 aagatggaaa aatagacatt cagcaatatt gggatgcaga ttttgaaaca aaagaaacgc
     6301 attcccgaca agaatggatc gaaaaaatcg acgaaaccgt tcaagcttct attgaagcac
     6361 atacggtcag tgatgtagaa gttggttctt tcttatccag cggggttgat tccagttatg
     6421 taacctctgt attgaaacca gatcattctt tctcaatcgg gtttgatgat aaaacctata
     6481 atgaagcgat tgaagccaga aaactaaccg aactattgga cttagataat acagcagcag
     6541 tgatcgacgg agacatgtcg ttcaaagcat tccctttgat ccagtatcac ttagatgaac
     6601 ctgattcaaa tccttcttgc gtacctttgt actttttagc aaatcttgct tcacaaagtg
     6661 tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt tgcgggatac caaacgtatg
     6721 gattccatac gaattcaaaa ttcatccgtg tgattgctca aggattgaaa aaattaccaa
     6781 aaggaacacg ctataattta ggacgtaaaa tcggaaaaat gaaaaatttc catggacgga
     6841 tccatttata tgaatcactt gctcctgcaa aagaatattt tatcggtcat gctcgagtgt
     6901 tcgaagaatc agaagcatca gaagtgttaa caccaaaata tcaaacagca ccatctgtgg
     6961 atgaaatcat gactgttcat tacgagaaaa cagaaggaat caaagatgaa gtcaataaga
     7021 tgcaatatgt cgatcttcat caatggatgc caaaagatat cttgttaaag gcagataaac
     7081 tttctatggc aagttctttg gaagtccgag tacctttatt ggatatcgaa gtcatgaaat
     7141 tggctcaaca aataccaagt aagtttttat tgaatcaaaa caacacaaaa gatattttca
     7201 gacaagctgc gaacaaacat cttccggaag aatggtcgaa tcgtgtgaaa ttaggattcc
     7261 ctgtgccaat caaagcttgg ttgaaagaag aacacggcta cgaacaagtc aaagcattat
     7321 tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa aatcatgaag ttgctagatg
     7381 accatcacga aggacgtaaa gaagagcaaa gaaaaatctg gacaatcttc agtttcttga
     7441 catggtataa agtctacttt gtagatgaaa cgattccgca agcagaagca atcgactatg
     7501 tgactgtttg atgaaaatca aatatcaata aaaaagaggt tttctcctac cgtttaacac
     7561 tgagtgataa gaaacaattt caaaaaacag ctcttcccgt tttatagata ttatgactta
     7621 tggttcggag ttggcagagg aacaccattg tttcgaacat aagggggttg tgacacgatt
     7681 ttcgtcacaa cccctttaca tatgttcaat aatcgatttc tttttctttt gtcccatctt
     7741 ttttataaat gaacaaagtt gattctttgt tttgtgctac ttgctttcct ttttcgattg
     7801 cttctttttt tgtatcaaaa tggttgcttg ccttttttgc ccctttagat ttcacgatcc
     7861 attgttcttc ctcatattct acgatcacat ctgaatcaag cagttttcca gcacgaggat
     7921 tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt ttcgaaagtt cgtttttctg
     7981 attcactagc atcttcatac catttttttg cttgatcaat ggcaattggg atcgcacggt
     8041 tatcagggta cccttcatct aataatgcat ttgcgatgtc gattgctttt tttcttgtca
     8101 atttatctaa gtttttcatt gatgcaggaa agtccttcat gttccatggc atattagtat
     8161 ccccctttat taaaaaacat aatctatata aacaagtgta agggtattta tacaaaaaga
     8221 aaaagatttt gtttgagaag taaaaccgtt gtatttatcg tcaagtagtt ttacttaaca
     8281 aaatcgtgaa aaacactagc aatctagaaa aaatcatggt aagataatca agtctgagag
     8341 tcatcttaga ggattcttgt aaaaatggga gggaaaaaca tgcgcgtaaa catcacttta
     8401 gagtgtactt cttgtaaaga acgtaactat ctaacaagca aaaacaaacg taacaatcct
     8461 gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca aagttacttt gcaccgtgaa
     8521 acaaaataat gtttttaaag ttctcaattc cttgctggtc aaagaattga gacttttttt
     8581 atctcaaaac atctaaaatc ataaggagcg gataatatgt ttatttccat gtgggcacaa
     8641 gataaaaatg gtctgatcgg caaaaatggt ttgttaccat ggcgattgcc aaatgatatg
     8701 cgtttctttc gcgaacacac aatggatcga attttagtga tgggaagaaa gacctatgaa
     8761 ggaatggggg atctttcact tccttatcgc catatcatcg tgttgacaac gcaaccaaat
     8821 tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg atgaactttt gtcgtatagt
     8881 caaacgatct cagaagatat ctatgtttcg ggtggtagtc ggatttttca agaacttctt
     8941 ccttacactg gtcggatctg gcgaacattg atcgatagca cgtttgaagg cgatacgtat
     9001 attgggaata ttgatttttc agacttcgcc cttatagagg agtatgaggg gataacggat
     9061 caagaaaatc tatatgccca tcgttttcaa aaatgggaaa gagtaaaaaa tagggttgct
     9121 tcaaaagaag aataaaatct atgcgtctag tttgttaata agaaaaaaag catctcacga
     9181 ctaatcgtga agatgctttt tttagttaaa atcaatttag tttttctttg atatcattga
     9241 ccatttcctg actggcaata ggtccgttat atagccaact agattcaggt ccgaattgca
     9301 gtacatgatt gtctgtcact gcttttagat tcttccagct tggttcattg aacatggcag
     9361 catctttgtc actgttcaca aggatgatgt agtcagcatc taattcagct agcttttcag
     9421 aggaaacagc agaccagtca gaagtggctt ttttggagac ctcttccact agatcaggaa
     9481 caccaaattc cagatcgtcg tatacgattc ttccacttga ccgatttttg ctgaccataa
     9541 aggctgagtt gttggtcacc cacaacactg cagctgtctt gttctcgatt ttatctgcta
     9601 gttcttctcg agttttcttg atggattttt gataatcgga aatgattttt tctgcctctt
     9661 tttcttttgc caaagctttg ccgacatctt ttagttgttc ttcccatgtg acatctgttc
     9721 cgttttttac aacataagtt ggcgcgattt tgctatattg ttcgtatttc ccgccttcga
     9781 cagtagcgga agaagaaatc aacagcaaat caggctcaaa gcttaacact ttctcataag
     9841 gaaggtcata agaaatcgtt gggacatcct ttaaatcatc ttgcagataa tcttgaatgg
     9901 aaccactgcc aacagtccac tgggcgatag gtttttcccc tagtgcaatc agatagtctt
     9961 ctaagtaact gccgatgatc cgtttgggcg cgtttggaat ctctacttta tgtccaagtg
    10021 tatctgtgac tgtatgagta ctattggatg aagtcgtcga attggcagtt gtagctgttt
    10081 ctttatctga agagggggag caagcgctca gtactccagc cagcagcaat atgctagata
    10141 atgtgaatac ttttgttttt aatttcattt ctttcctcct aacataattg agaatctttc
    10201 tcattggtta ttattcctga tatttcgaac gctgtcaacc gcttcatttg gctatagata
    10261 tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa ttgagaatga ttttcagcga
    10321 aaggatgggt gaaatgacga gattaagagc agctgacatt gcagccggct acgaaaacaa
    10381 aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg aaaatcacta gtttgattgg
    10441 gccaaatgga agtggaaaat ctactttatt gaaaagtttt acccgtatac tacctgtgaa
    10501 atcaggaaag atcattgtca ataactctga tatctctaca tacagttcaa aagaacttgc
    10561 aaaaaaaatt gcattattag cacaaactag tgaacaccct acaggtatga cagtagaaga
    10621 agtagtatct tacggacgat acccttatca aaaactgttc agtggcatgg acacgcaaga
    10681 tcatgaagca attgagtggg cgttggatgc tacaggtcta gctggatttc gtatgcggga
    10741 tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg atcgctatgg cgcttgctca
    10801 ggaaacagat atcttgatct tggatgaacc gacaacattt ttagatccgg cacaccaatt
    10861 agagatccta cagttattga ctgaaatcaa tcaaatgaaa caaacaacga tcttgatgtc
    10921 gattcatgac attaatcatg cttcacgttt ttcagattat ttattagcaa tgaaaaacgg
    10981 taagttgatc acagaaggta cacctgaaga agtgatcaca aaagagtgta tgaaaaaaat
    11041 atttgagatt gatgcaacga ttgtcacgct tccaagaaca aataaaccag tttttctaac
    11101 ctatgatttg ttgccaaaag agcaggagga tcaattatga ggactatgaa gaaaaataga
    11161 ctcctgcttt tggtctgttt tgttcttttg attatttttt ttgttgctct ttcaaatggc
    11221 gcagcgactg tgggaaaagc agatttttgg caggcgttgt ttgattttga tagatcaaaa
    11281 caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg tggcgggagc atttcttgta
    11341 gggagttgtt ttgcattgag cggtgcattg atgcaaggag tgacaagaaa tcctttagca
    11401 gattcaggct tactaggaat caacgcgggt gcttcgttgg caatggccct tagttttgct
    11461 ttttggccta aagcttctga tttttctgtg tttctaatgt cattggcagg ttcttttgct
    11521 gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa tgggcatgaa tcctttacag
    11581 ctgattttag ctggcgtagc tattagttct ttttttactg ctatcagcca agcacttacc
    11641 caattcttta atctgcagca ggatcttgct ttttggtttg taggcggagc tgccaatatc
    11701 acatggaaac aattagcagt attgtttcct ttatatatac ttgctgtttt ggtttctttt
    11761 tcattaggaa aagcaatatc tttgattcat ttgagtgaaa cacatgcatt agctcttgga
    11821 agtcatccta aacgaacgcg gttgattgtt ttctgtctag ttgcagtgtt agcagccatt
    11881 gccgtttcgt tagtcggacc tgtttcattt attgggctgt tggttccgcc agttgttcgc
    11941 ggaatggttg gaacggacta tcgttatgtt ctacctgtct cttttttagt tggcggtgga
    12001 ttggttatgt tggcggattt tacagcgagg atgatcaatc ccccttttga aacacctttt
    12061 ggattagtca ttgcattgat tggcgttccg tttttactga tacaagtaag gagggaacaa
    12121 cggtgaaaaa aatatattgg acgttctatt tcttgctttt tgtacttgtc atcctgtcta
    12181 taggtagttt aatgattggt acgcctttta tttacctgga tcagcttttt gccacgttca
    12241 tagggaaaga atcatcttct ttggcgctga ttgttagaga atttcgagtt cctcgattac
    12301 tgatcagttt attagcagga gcttgtcttg gcgtttctgg ttatatcttg caaggagtaa
    12361 caagaaatga attggcagat tctagtatat tgggcattaa tgccggagca ggatttttag
    12421 tgatgcttta tttaggtttc ttttcgcact attcgctgcc gtttctattg ctagttgtag
    12481 cctttctagg tggtgtttta gctgcttgct gtgtctatct tgcggcctat gatcgaaatg
    12541 gctttttggg tatgaatcga atcttattgt ccggtattgc cgttaatgcc ggcctttctg
    12601 cattaacatt gctatgcacg atccaattat ctaaggaaaa ttatggattt gtgaatgcat
    12661 ggttagcggg atcaatctgg ggtgcgagct ggccttatgt ttgggcattg cttccatggg
    12721 cggttatctt aattccgcta ggaggacttt atgcacagag gattgtattg ctatcatttg
    12781 gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa aagtcaaaaa atccttttgt
    12841 tgttagctgt tgctttggca agcggtagtg tagcggtgac aggtagttta tcttttgtag
    12901 gactactagc tcctcatgct gcgaagctag tcgtacgtaa agaaaataac tggacctttg
    12961 ttctgagcgg tttgttcggt tccatttttc tgcttagtgc agatatggtt gctagagtga
    13021 ttctactaag tggggaagta cctacaggaa ttttgattgc cctttttggt gcgccatatt
    13081 ttctgtatgc actttttatc agacaaaaac acgcgctgaa cagttgaaat tcattaaaaa
    13141 aggaaggcca atcatttcta ggtcttcttt ttattctatt tatgcattta tttgaaagtt
    13201 cgattgctat ttttcttttt ttcctatacc attttatgag agaaggaagg gtgagttttt
    13261 ggaccaattg aaagtaagat tacgccggaa aggaatcgaa aagctgaaaa atctggccaa
    13321 aagtggcaag aaaaaggaaa aagaacagcg aatctataaa cagctattcg ccagcgagta
    13381 ctggaagtca gcaaaagtga tagggacgac tttatcgaat gaatttgaac tggatacaca
    13441 gccggttatc caacgagcag aaaaagaaca aaagaaaata gttgtaccta aaactttgcc
    13501 gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc gttcttgtac gttcgagttt
    13561 tggtgttctt gagccacaat cagagaagct ctatttgccg gagatgatcg atctcctcat
    13621 tgtaccaggt attatctaca atagagccgg ttatcgaatc ggttttggcg gtggttatta
    13681 cgaccggtat ttaaataatt ttcataaaaa aacatgtagc cttgtttttc gtgaacaatt
    13741 gaatgaacaa tggcaaccgg agagctttga tcagaagatc caacaattgt ttattgacta
    13801 aaatgaaatg ggaggttttc atgaattatc aacagcaaat aaaattgcgt gcatggttaa
    13861 gacgaccgtt atggacctat gcttttttag ggatacagac agcggtcttc ctcatgatgg
    13921 aattatttcc gcgacttgag atcccttatt atgccggaat gtatggtcct tatcttgttc
    13981 acttcaatga atggtggcgt ttagtgacac caatcttcat ccattttgga ctgatgcatt
    14041 tcgtaatgaa ctcactgata ctgtatttta tggggcagca aatcgaggca atctatggcc
    14101 attggcgttt tttcttgatt tatatgttca gcgggataat gggaaataca gcaagctttg
    14161 cttttaacga agcgaatgtt ctgtctggtg gtgcaagtac ttcgatattc ggtttgttcg
    14221 gtgcgttgtt tatcttaggg tttcatttta aacataaccc agcgatccag cagctagtca
    14281 gacattttct tttatttatt gtgttgacat ttgtttttgg tcttctggat acctctgtag
    14341 atgtttgggg acatgtcggt gggctaattg gcggattagt gttagggaat gttttgggtc
    14401 taccgaaaca acagacaagc tattcgatac accaaaggat tctttctaca ctagtttttg
    14461 tatttctttt tgtaatatgt attttactgg gtttaaaaaa atatggttta cttgtataat
    14521 atgaaggaag tgcttaaaga tggagagctt atatgatgta cagcagctgt tcaaaaaatt
    14581 tggcatcttt atctatgtgg gtgcacgcat ctatgacatt gaattaatga ctatcgaatt
    14641 gcgtaatctg tatgatagcc gtttgattga tcatgataca tacatgacag cgtggcgcat
    14701 attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa ggaaatttgt aatggacaag
    14761 aagattattg ggattgattt aggtggaaca acagcaaaat ttgcaatttt aacaccagaa
    14821 ggagagattc agcaaaaatg gagcatcgat accaatattt tggatgatgg taagcatatc
    14881 gttcctgaaa tcattgaatc aatcaatcat cgtttgaatt tatataatat gaaagccgaa
    14941 gactttatcg gaatcggcat gggtacccca ggaagcgtgg acagcgaagc tggaacggtt
    15001 attggtgcgt ataacttaaa ctggacagaa gttcaatttg tgaaaaaact gatcgaggca
    15061 ggaacaggaa tcaaattcgc tatcgataat gacgcgaatg ttgctgcttt aggtgaacga
    15121 tggaaaggtg ctggagaaaa cgatccagac gttgtattcg ttactctagg aacaggtgtt
    15181 ggcggaggta tcgttgctgg cggaaatttg attcacggcg tgagcggagc tggaggagaa
    15241 atcggacata tcactgtgga tcctgaaggg ttcgaatgta cttgcggtaa acgcggatgt
    15301 ttagaaactg tatctagcgc tacaggtgtg gtacgcctag gtcgtttctt ggcagaagaa
    15361 tacgcagggg attcaaaact gaaagcaatg ctagacaacg gagaagaagt aacaagtaaa
    15421 gatatctttg aaatggctca agaagacgat ccgtttgcat tgatggttgt tgatcgcgta
    15481 tgcttctact taggtttagc ttgcggaaac ttggggaata cattaaatcc gtcaagtatc
    15541 gtgttaggtg gaggagtatc ggcagcagga gaatttttac gtagccgtgt agagaaatat
    15601 tttaaagaat ttactttccc gcaagtaaga gaatcaacaa aaatcaaatt agcagagctt
    15661 ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct tgaaatttgc ttaaaaaaac
    15721 ggaggcatta catggtttta tgggtaatta atatcatttt gctattgatt gtgcttgcaa
    15781 tcatcctttg ggaagtctat ctacgagtaa tggccaaacg ttcagcaacg actttgacag
    15841 aagaagaatt tcgagaagga atgagaaaag cacaagtaat cgatgttcgc gaaaaagatg
    15901 tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc ttacactgtt ttgaaagatt
    15961 cattaggatc tattcgaaaa gaccagcctg tctatattta cgatcaaaag aaaagtttga
    16021 gcatccgtac agcaaataaa ttacgtaaag caggttatca agagatttat ttacttaagg
    16081 gtggatatga cggatggtct ggaaaaatca aaagtaaaaa agtataaaac agatgattgt
    16141 atgacagctt taaaaaccga atcaatcatt agattttgac caagatcaca tgaacggcgc
    16201 aatggatagt tgtgccgttc atttttttta ttttgtgcta ttatctacat atataagcta
    16261 agcgaaaagg tagaaagaag gaaacaacta tgatcaaatt aaaacgtgtt taccttgatg
    16321 ctgaaaaaga ggatggctat agaatactcg tggatagatt ctggccacga ggaatttcaa
    16381 aagaaaaagc gcagttagat ctgtggttga aagaaattgc acctagtaat gaagcacgca
    16441 aaacttttga ccatgtacca gaacgatttg cttcatttgc aaagaaatac caacaagaat
    16501 tgattttcga agaagaaaag aagcaagcta tttttaaact cagtcaactt atgaaagaac
    16561 atccagtcat tacttttgtt tatggtgcaa aaaatgaaca tcagaaccat gctgttttat
    16621 tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata gggatcattg gtgccggacc
    16681 tagaggatta agtgtagcag aaaggattct ccataataat caggacagac attctcttga
    16741 actttttctt ttcgatccca atggtcctgg aggtcgagtt tggcggttga atcagccacc
    16801 ggagttatta atgaattctg tttctcaaca agtgacgctt tttacagatg aaacattgtc
    16861 cagtggagga ttggtaacac agggacccaa cttgtttcaa tggagtaaag gacctgctac
    16921 agattatatc gaacagacgg taaccaaaaa taagcaacta attatagaag aaatcaatcg
    16981 tctggaaaaa gatcagcaat ctactcgatg tttatacgga ctctatcaac gctggttttt
    17041 ctcaaagttg caagaagaat ttccagcttc tattcagttt gtttattctt tagtaaaaaa
    17101 agtaagcaaa agaaaagatg gttatctttt acagacagat cagcaaagct attctatcga
    17161 tcagcttgtt ttagcaagcg gtcactggga aaatgatgag acagaagaag aaagagtgtg
    17221 ggtagactac gcaaagaaaa atgttttact gtatcagctt ccttctaatc ctgcagatgt
    17281 agatctttcg aagataccgc ctaaagaaaa tgtcttttta agaggtctag gcttaagttt
    17341 ttttgattat gtcggattgt ttacagtatc aagaggaggg cgcttcgaga acgaaaaggg
    17401 aaaactggtc tatcatccat caggcaagga accgattgta tacagtggat caagaagggg
    17461 gctcccttat taccctcgag gaagaaatca aaagcaaggc ggagcaatgg catggccacg
    17521 gttactaaca aaagaaaacc tggagcaatg gcatcagtcg ggatttttga ctggcgaaac
    17581 attttttgac tatctgaaaa aagacgttga gttgttttat tacaagaaaa tcatcgaaga
    17641 atatcacttg ccgattccaa agaaaaattt tgagcatgat tttctctccc tctcttcaga
    17701 ggagtgtctc ggaaagcatc cagcactaat cccttataga tgggattggt cgtatttaga
    17761 aactcctctt gcctatcaga atgagccctt ttcagaagct agccgaatat ttattgaagg
    17821 gcagataaaa gaagcggaaa aaggcaattg cacaggagca tttgcttcaa catttgatgc
    17881 attaaaagac tggcgtgatc caatccggca ggctattgaa tggcacgttt tttctgctaa
    17941 ggaatacaaa gaaatactat ggggatggtt tactcatcta aatagctttt taacgatcgg
    18001 tccgccaatc attcggacga aagaattagc agcactgata gatgcgggta tttttcattt
    18061 gatcgaaccg ccatttgata ttcagcaaaa gcacaaccgc tatgttattt ctctacaaga
    18121 taaagaaatc agcagtcgct atttgattga agctcgattg ccacaaacaa gcttttctcg
    18181 ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa cttgttcgcc ctttttcatt
    18241 ttcagataat acaggaagtt atcattcaga agctatagat gttgagcgag gtacgaatcg
    18301 cgtgattgat gcttcagggg aaatccagtc tgacttttat tgctatggaa tacctgttga
    18361 aggtgtggat tggctaactg cagcagtagc tagaccttat acagatccat ggaatctgag
    18421 acaggcagat aaaatagcac aacttatatt gactgacaaa taaaaatacg catgatgact
    18481 tatctgtttt ttagaaagtc ttcatgcgta tttttaaata attattaacg agaaacgcgt
    18541 ttacgcattg cttttttac
//
LOCUS       DACEND010000047        17857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEND010000047 DACEND010000000
VERSION     DACEND010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.048"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..221)
                     /locus_tag="IW746_001360"
     CDS             complement(<1..221)
                     /locus_tag="IW746_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1078267.1"
                     /translation="MKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKTIDWIKWGTLE"
     gene            527..715
                     /locus_tag="IW746_001361"
     CDS             527..715
                     /locus_tag="IW746_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1078268.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            761..1138
                     /locus_tag="IW746_001362"
     CDS             761..1138
                     /locus_tag="IW746_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ1078269.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(1698..2870)
                     /locus_tag="IW746_001363"
     CDS             complement(1698..2870)
                     /locus_tag="IW746_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078270.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(3114..4082)
                     /locus_tag="IW746_001364"
     CDS             complement(3114..4082)
                     /locus_tag="IW746_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1078271.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(4079..4276)
                     /locus_tag="IW746_001365"
     CDS             complement(4079..4276)
                     /locus_tag="IW746_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078272.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            complement(4287..4604)
                     /locus_tag="IW746_001366"
     CDS             complement(4287..4604)
                     /locus_tag="IW746_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078273.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            complement(4641..4859)
                     /locus_tag="IW746_001367"
     CDS             complement(4641..4859)
                     /locus_tag="IW746_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078274.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(4968..5159)
                     /locus_tag="IW746_001368"
     CDS             complement(4968..5159)
                     /locus_tag="IW746_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078275.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(5321..5677)
                     /locus_tag="IW746_001369"
     CDS             complement(5321..5677)
                     /locus_tag="IW746_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078276.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(5702..6706)
                     /locus_tag="IW746_001370"
     CDS             complement(5702..6706)
                     /locus_tag="IW746_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1078277.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(6743..7003)
                     /locus_tag="IW746_001371"
     CDS             complement(6743..7003)
                     /locus_tag="IW746_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078278.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(7012..8085)
                     /locus_tag="IW746_001372"
     CDS             complement(7012..8085)
                     /locus_tag="IW746_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078279.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(8093..9919)
                     /locus_tag="IW746_001373"
     CDS             complement(8093..9919)
                     /locus_tag="IW746_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1078280.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(10074..10940)
                     /locus_tag="IW746_001374"
     CDS             complement(10074..10940)
                     /locus_tag="IW746_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078281.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(11115..11378)
                     /locus_tag="IW746_001375"
     CDS             complement(11115..11378)
                     /locus_tag="IW746_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078282.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(11380..11709)
                     /locus_tag="IW746_001376"
     CDS             complement(11380..11709)
                     /locus_tag="IW746_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078283.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(11731..13266)
                     /locus_tag="IW746_001377"
     CDS             complement(11731..13266)
                     /locus_tag="IW746_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078284.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(13333..13800)
                     /locus_tag="IW746_001378"
     CDS             complement(13333..13800)
                     /locus_tag="IW746_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078285.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(13797..14093)
                     /locus_tag="IW746_001379"
     CDS             complement(13797..14093)
                     /locus_tag="IW746_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078286.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(14444..14674)
                     /locus_tag="IW746_001380"
     CDS             complement(14444..14674)
                     /locus_tag="IW746_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078287.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(14710..15606)
                     /locus_tag="IW746_001381"
     CDS             complement(14710..15606)
                     /locus_tag="IW746_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078288.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(15620..16270)
                     /locus_tag="IW746_001382"
     CDS             complement(15620..16270)
                     /locus_tag="IW746_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1078289.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(16285..17421)
                     /locus_tag="IW746_001383"
     CDS             complement(16285..17421)
                     /locus_tag="IW746_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078290.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(17443..>17857)
                     /locus_tag="IW746_001384"
     CDS             complement(17443..>17857)
                     /locus_tag="IW746_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1078291.1"
                     /translation="KTEIISNSYLSVFINSNNKYACEYVLSFEREMRKMRAGVYFATQ
                     TPEEIIPKDSGSEVADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLP
                     ELEVGQAVVQTSSEDTYMINFDPYPEQIERFDGGQ"
BASE COUNT         5168 a         3403 c         2127 g         7159 t
ORIGIN      
        1 tcttctaaag ttccccattt aatccagtcg atagttttgt tacttaattc agcaatttta
       61 attaatcttt ctttgttggg aagggagaat cctcgttccc aattattaat atttcctttt
      121 tgagcatttc caattaaatc tgcaaattgt tccatagtca ttccaaaatt agaacgaatt
      181 gatttaattc ttttacctgt ttcaacagga tttggtttca tacaatcacc tcttttacag
      241 aatttggttt catagtatct tattcgctct ttgagttcta agttctcttt ctccaatttt
      301 tctatataat attcttgatc ctgtttcttg ttcaaaaaat gacctccaaa tttcacattg
      361 tgttacaccc tagtgttaca cctccaaacc ttattatact gtactttacc atatgataca
      421 atagggtata ccctattgtg acacttacat tctactttta atgtaaaaac tcttgacctt
      481 tatacgcata atgcgtataa taatatttgt aaggaggtag tcgcgaatgc cacttaccgg
      541 taaaaaaatg ctcaaactag ccaagaaaaa tggttggaaa gtggtgagga taactggcag
      601 ccatcatatt gtagccaaag aaggattccc acccgttcct attcctgttc atagtaatca
      661 ggatttaaaa aaagggacag agcagaagtt actaaaggac ttagggctga aatagcccag
      721 tcctttagtt cattggatta actcgaaagg agtggtggaa atgaaaaaaa gttaccctgt
      781 aattttttct gctgaagaaa atggctactc agtagttttt cctgattttg ctggagcaac
      841 acaaggagat aattttgaag aagctatgtc caatgctaaa gaatttcttg atgggattct
      901 agcttactat attgatgaag atatttcagt tccttctcca acagatattc atcaactaaa
      961 taatttgggc aataacaaaa ttgtttctct tattcaaagt gatccttctc catttatcca
     1021 aaaaaagacc gtacgcaaaa acgtcacagt cccagaatgg ttagtagcta gaggggaaaa
     1081 agataaaata aatttttcta aagttttgac agatgcgtta ctaaaaatat acgagtaaac
     1141 gtaaatatat taataacaat ttagttgcct aatgacatag atagcggaat taaaagtaaa
     1201 atagtactca tttattgcag gttcccttag aatcaataca ataaatgagt actatttaga
     1261 ctgtttaact actagtaaga tatagcgatc atcagtttca gggttatccc ttggtgacca
     1321 tagttttgcg tatttttcaa ctcgttgcca aatcaacgat tctatttttt tatttctact
     1381 taactccaaa acggagtacc ttctttttct tcgttagctc ctatttaaga gccatgtcgt
     1441 tttatataac tctaaaacag aaactactga tcaattcaat aaagccttat ttgagaactg
     1501 tgttgtttta catggtttta aaacctgatt tcccgcaaac ggtttatccg taacatttga
     1561 gaactgtgtt gttttacatg gttctaaaac ctcaacaata ttcgtccttc cgtctggtta
     1621 cacaacttga cagagctaac ccttcagtga gtgagtgtcc cctcactatt tcgttgccaa
     1681 atcaacgatt tcttattcta ctcactttta acttctattc catatctttt ccctttattt
     1741 attatttgtt ctttcaactg ataataagcc cattccccat aatttttatc tggttcagaa
     1801 atacagatta tttgcgtctt attgcggata gctgtttgaa taatttttgc cgaacattga
     1861 ttattatagg ttttaacaaa attgctttct cttttcttta tggcttccca tctatcttga
     1921 tctaattttc tttttcgacc atgaccgcct ttagaaaaaa ctcctcggct ttgctccctt
     1981 gatttcattg ctttaatttt atttttgaag ttacttatat atttgctgtc accaaaatca
     2041 atcacagatt gattgttaac taaacattta ccgctacaca aatcagtaaa ctttatggtt
     2101 aattcagctc cttctttgaa atctagctta tgataatctt tttcaaaaga aattgttaaa
     2161 ttcaaaaata aatttttccc ctcaaagatt agattactct ggtttaactt gtattgaccg
     2221 tccataatgc gttctaaagt taaagcgcta tctctggctt ttcttggttt tctacctaaa
     2281 tttactttaa acctaaatcc tttaggtaaa gagatataaa acttgtcagc tttgtgaatc
     2341 ttaatatccc gacctctgaa cggtaacaat ccatatttat ttttatttct atagattcta
     2401 ggtgttcttt gcccagtaac aattccaaca aaatcattgc taaaatcctt actagctttc
     2461 attttagctg aatctataaa atccgaataa tcaaagtcca atttttttgc tatatcacgt
     2521 attctggttt gactttttaa acctatagct tgtgtcaaaa cgtctctggc ttctttgttt
     2581 ttcgcaattc gtagtttaga taattccctt ttcgttttat ctaatttctt ttgatcgatc
     2641 acttcattac ttagtaaatt atcaattcgc tcttcaagac ttagctctct tttaatatac
     2701 tcatgatcca attcttttat agaattttcg atcaattctt caaacatcag ctgtttatat
     2761 accatattca gcgcttttac atattctcta ctatttttcc aaaaaaaacc aaaaaacgaa
     2821 tctctttcct cttgtgatcc atcaacatat acttctattt ttttagtcat agttattatt
     2881 gtcaatttta gcacctcatt attatatatc tggtatataa taatagtaac atatttccta
     2941 gactataatc aatgatcaat tgtctacttg tagtaattat attaaaaaca aaaactaaaa
     3001 ataatatatt attccttctg actacatata gtcactttat ccaaaaaata agaagtgctt
     3061 tcgcactcct tattaaattt atttactatt aattgattca gctacaactt cttttattca
     3121 acttcaaaat cagttttttg gatatttcta tctctattta attccatact taatagctca
     3181 ctatcagttt ttactaaaag ctcacctaca ctattaatat atgccaattt acaagaattt
     3241 cttcttttat cttgacctac taattcatct tgagataccc ctgtagaaaa actaattgat
     3301 agaatccgtt ccttgatttc atttttatgg ttcctataaa aatcatcgac ttgcgcttct
     3361 tttgaaaacc gatcactaat aaaatcatca cttgctgaat atattctttc aagggtttct
     3421 tttggattca tctcttcttg agatatatcc atcataattt gtttgcttat aggagtttca
     3481 gcatttttct cttgaatagc tttgggaacc caagcatatt cttgcgtatt attggctaca
     3541 actgtatgat tttctttaac ataaatataa gaaaactcat ctgaattatc atagatactt
     3601 acattatcaa aatatttctc taactctgct aagtttctta aagaaaattc gtatcttttt
     3661 cttattaaat cttcaggtac accgtggcca cctttagcaa cacggatttt aaccctttca
     3721 atcgctcttt ctggtgaatc tagtcctata tagattaaaa acgattcata acctaatttt
     3781 ttagccttta gaagtctcat tttatcggga tatggattac caccaaaagt cgtttcatgg
     3841 tgaaaagatt ttccttcttt aaaatgacct ttgatctctc ttagaacttc acgcatactt
     3901 ttgtttatgt ctgcttgttt tttccaattt ccgccaaaac ttctcaaaat ttcatctgca
     3961 ttaattctat cagtatcaag ttcttttctt tttaaatttt gaacttgata aatagtagac
     4021 ttaccggcgc cgttaacgcc cccaacaact acataatacg gtttttcatt ttctaaactc
     4081 atattttaaa gtctttctga ttaattactt ttttttgatc tactcgtgtc acaaaactag
     4141 aaagtgtaag gtagaaagtc ctttcttcct cactttctgc attttttaag gcttcatgaa
     4201 aatcatctac agtcatattc aaaacatcag gatacatttt ctttaattct tttacagcct
     4261 ctgtcatagc tctcattccc ttcttattag tctttattaa agtactctgc ttcagcttct
     4321 agtctatctt tcaagtcttt taaaacaacc gtaagtatat gatcttcacc atttttttca
     4381 attgattgct ttatcgcttc atctaactta tctaaatagt cttctataat tcctaaattg
     4441 tttacttcga taaatcggaa gtccggtaaa tttttcatcc gaataccgcc tttaggctgt
     4501 tcctttacaa ataaagattt gtaatcatta tattcattcc cattaataac caattctttc
     4561 ataggctcta agtggataaa tttacgtgaa gcatagtctt tcataataat ctctcccagt
     4621 agaaaatctt cttttttcta ttatatcacg ttctcaaagc gttgagttat tggattgttt
     4681 aaaccatcaa gacacatcat gctgcatttt ttcatcacta ccggtgcttt tccgattgct
     4741 ttaactaccg ctacattagc taacaaacta attacttctc cctcgataag ctctccgctc
     4801 ccaataacaa cgccctgtac ttccatacct actttaaacg tttctttttt atttaccatt
     4861 attccgctct ccttccatga ctacttgtag tcatttggat attttttagg ggaacggttt
     4921 tccgttccct ttcatcaaat agaatccttc atcatttcaa caaataatta actttcagtg
     4981 taaaaaaatt cttcgtaatt accatcatga aaaatatccc atctttgttc gtcattctca
     5041 ctgaagtagg taaaataata tctgacagta tctaacttat ttaattgctc tctggttttg
     5101 taaatacaat ccaagtgatc ttgcattcga tctgaccaaa acattcttgt ctgctccatc
     5161 tattatctct cctttttatt aaatcaaggg tttgagtctt tgactacttg tagtctatgg
     5221 ctatatcata caggaatgac tacttgtagt caatggtttt ctttagttta attaactgtc
     5281 tattcgtagc caaataatgt aaaaaaacgc ctagtttaca ctagacgttt aaacaattca
     5341 ataattcttc gttatacccc attaagggtt gaactgcttt aatacttact tgatcgtctt
     5401 ctctaacggc ttctatgctt accccttttg tatattggcc atctttctta tccgtccaaa
     5461 tttgataata agtaagccca tatcgttcaa taatctcaaa gagaatagtt attaattttt
     5521 tttgtttaaa ttttggtaat tcaatacgca atgatttttc ttgaatatcg aactctggat
     5581 ttttattttt aagttgttct tcaataatca tatagtctgt agacgtctta tctaaatcaa
     5641 ttaatgaatc tgataagatt gctattgtat cgttcataac acatcaacta ctttcttctt
     5701 tttattccat ttcatataca ggagtattaa gagataattt atgtgtgaag ttcacgattt
     5761 ttgatacatc ttctaacttg aagcgtttaa aagcttcttt tcctcttgtt aaattatcat
     5821 tccaatcaaa tccctctcgc aaaggtacat cgacttttac ttcactgatt cctatccctt
     5881 ctcttccaat tcctttttct ttaaattcag tttcaataaa agtacgtccg gctttatcat
     5941 tatcaacaca aaggactgtt tctgaaggca acgatttaaa cttattataa tgctctgtaa
     6001 ccccatgcaa tacattacct acctttaatc cattcatgct aacaaacgta gcattttcgg
     6061 ctttttctgg cttttgtgta atgtacgaca tcaaatctat ttcactttca aaaaaataca
     6121 ttttatctgg ttgaccaaaa gttacgttaa atcctgaata ctctttagag ttttcttgta
     6181 tttttttcca tgtaacatcc ttttgtttgc cttcatgaaa aaattttcta gttccttgtt
     6241 ccgtacaccc aacgatttct tgatcttttt tccataagaa caaaacattt ccttttttat
     6301 cttgtttaat tagtccttta gataaaaaat cttccactaa agctgtatcg attcttctct
     6361 catttactaa ataagacttt gcaacagata ccggcacttc attttctttc tgatattggt
     6421 acggagcttt tttcactttt gtattgaacg aatgaacttc ataatcacta tcttcaagtt
     6481 tctgttttgc ttctttaaat gaacaatcgt agtaggcttt gataaaatca aaaatatctc
     6541 cccctaaatc ctttgagttc catttaaaaa ggtttttacg atcactgatt acacaagaat
     6601 catgatcttc taatctatag tattcccttg aatcttgctt taaacctatc ccattttgtt
     6661 tgcaaaaatc aacgatactt actttttttg cctttttcaa atccaaacaa aaaccccctt
     6721 tactagacca tcactagtaa aattacgata aggtacactt gtatttttca taataatcct
     6781 ctgtacagaa aataaagttt agcccatccc taagagataa ctcccaaaag cctatcttct
     6841 tgctcttttt ttctctaatt atcttttttc taatactttt tccgtacttc aaagccattt
     6901 taaaagcgat ttgtacatct tcacaaatta caaaatgact attattaatt tgacgatatt
     6961 caccttgcgt ttctaaaaaa tttctatttt cttgttctat cattcattta tctactccat
     7021 ttcaaatttt gttgtatttt caaaattagt caatttcaaa atcttattct ccatttcttc
     7081 agaaacaggt ggtatttcaa acgtatcatt ttcaatttca ttataaattt ctctcattgc
     7141 accgttgtaa gccatgatag acgtagcttt tttgtattcc gtagaagaag tatctactgg
     7201 aaaacctagc agttcttctt tatcgatctc taacttttcg gataaatttg tcaatttcgt
     7261 ttcaatttct tctttatgac tatcataaaa ttcattaata tcactatcta atgctaatct
     7321 ggaaactggt tcttccatgt tttctaagta ttcaatgcgc tcttgccaag cttgtttgtc
     7381 ataataatta gttgtttcta gctcttgctc aaaatctttt atcacaactt ctcgaatggg
     7441 aacttccgaa accttatgcc caacaaattt atctgtcatg tcctcactac tttctaagct
     7501 attccgttcc tcgtttaaat cgtgaatagt ttcaatcttt actttatcag gctgtaacac
     7561 tacgataact acatcctttt gtcttaactg ttcttccgca ataatatgtg tatatttccc
     7621 aacgtcataa aatgccttag cagtagcttc atgatctcca attgtatttc caatgtcgtt
     7681 aatcaaatct acgtcatttt cattctcact aagagaagca atcacttctc tttttacaac
     7741 tttttctccg taataactaa catcattgta atcatttaaa atatcattac tttcttgtaa
     7801 tttatctatt actttagaaa gctcttcatc agtaatagtg cgttcattca tagaaatcgg
     7861 cttctctaat tcaacattta cttcatatac gaaaccatta tttccgttac taccctgttc
     7921 agcgtacatt gtagccattt ctttattagg tgttaagtac acaccaaatc cattagctgt
     7981 tccgtttaaa cctttatgat ctaaactaaa ttcatcaaat ttattcatac ttccatgaaa
     8041 caggcgataa gttccgcctg tttcataatt ttgttcatta ctcataattt tttcactcca
     8101 tttcaaaaga acgttctgct gtttttacag tcggcttttc caagttagaa ttttctggta
     8161 tcttgaaagt tccttcatcc atttcatatt ttactttagt tgcaaccagt ttataagcta
     8221 ccactgaagc cacctcttta tatgactcat tgtctattgt atctcttaaa tttgttcctg
     8281 acacagattc aaaatctgta atcgtatttt cgatttcttg tttatgagat tcgtaaaaat
     8341 cttccgcagt catacaagag tttaattctt ttacatcatt aatatttgtt tggttttcaa
     8401 tttcttcttg ccagcatttt tcattatagt aattgttagc attttcagat agattataaa
     8461 ccgcttcatt tataacaact gatcttagcg gttcgcttaa ttttaacgta tcatttttta
     8521 gttcatgatt ctcgcaagct ttcaaacttt ctacaacaaa cttttcaaca taatcacaat
     8581 tttcaggtga tcgatctaat agtaaacgat catgtttatt ttcacttgga ttctcaaaaa
     8641 ctacaccata aacatcacat acatcaaatt gtttcgtatc tgaataatat gtcacatagt
     8701 tttctcctat tttagctgta acatcataat tgctatgagt aagctcaata gaattttctc
     8761 catactttcc tctataataa tccaccatcg cttctaaata actaccgtgg attgttctat
     8821 ccggatcgtt gattgtacta tcgtagaaat tatgaaattc taaatctgat ttttgttgta
     8881 tctgctcttt attctcatta ggaacaaagg aattttttag tgcattaaat gctgtttgct
     8941 gttcttccaa gactttctta tcaatcgtac ttataaagtc aatagacgtt tctctaactt
     9001 cttgcattat ctgtctaaag ttttttggat caattttttc attattttta gtccaatttg
     9061 caatataagg aatagtaaag tctgacgtat caataccgta attgtgacaa acaataaatg
     9121 aagtcatttc cgcttgaaac tctttaatcg gtgttgagta ggaagaaccg tgttcattct
     9181 tattgtgaag ctttttgtga gccaattcgt gaatactggt agtaagattt tgtgttctgc
     9241 tatttcttga atttaataca atttcttccc taccatcaac atattgaata tattcacctc
     9301 tagcgcttcc catttcacct ctaccagact cgttcatatc ttttatagat atattcatag
     9361 attcggccaa atgatttaca cctttttcta attgactaac acttaattca ccatcaatat
     9421 taaaattcca taccctgtta ggaaatattt ttggcaaatc agcttcagtt gcattagttt
     9481 gggaaatatc aaatacgtaa cccttcttaa aatgcttgat ctttttgctt cttaattctc
     9541 catttttgat tttttctttt tgctcgtttg tcgcttcttt taacgtcttt tcctcaccat
     9601 tttcatcaat aaaaaccgtt accggagcat gcgcataaat gcctatccct ttttctttcg
     9661 ctttaacttg aaatcctagt tttttccaac catcataact tgctacagca attgctcctt
     9721 caaatccctc atcgatcaac gataggttta aaggactgta attgtgtatt ttagacatga
     9781 acttggcaaa ctcgatcata tcttcagggt tagactgata ttgctcgatt ttgctgttgg
     9841 aagccttaat taaatcgtct agctcttttt gtttttgctc tttcgttttc ggcttccagt
     9901 ttcctttttt ataggccatc ttctatctcc tttctttttt aggtaagtta aattacctaa
     9961 ttaaattata ccagatatat aaaaactctt ctatctaatt tcactgactt aatgcaaata
    10021 aaaaagagat aagatagtta tagtaaccat cttatccctt cttattttac catctactca
    10081 actatactta cgtttttacc tttactatct aattgaactt tgttatcttt agaagctact
    10141 ttagtaagca aaagttgatc caattcatca ttagaatatc ctgaataaaa attatctaaa
    10201 actttcgttg tggcaactgg aattgataac ttcaaatcca tactagtaga ttcataagat
    10261 aattctcctt cagacgaaac ttcaccaaaa ctatttaaat tccaaattgg actatctaaa
    10321 tatttataag ataatttaac ggtttccatt gaaggtgatt tgttttcatt atccatttta
    10381 attgttgctg ataccatttt ccactccggc tctgaatatt gaacaccatc gatctcttct
    10441 atatccgcct gtattttagg tatactatct tgaaaatcag caattttttg gtcattttga
    10501 aactcttgtt tttcctcatt ttctaattct gtttttacag aatctaaagt ggcattatag
    10561 ctatcaatat cgcttttttt cttatcatta aacttttgtt tatcagcgtt caatgcttgt
    10621 tctattattt cttcatcact cattttagaa actttttcca atgtaagctg ttccatatcc
    10681 gttttaaata cttttgcttt gccatcttta gtttggatca ccgcttttac atattcattt
    10741 ttatcaatag gcgttttatt tttactactt gtttcgacta ccaaccaaca acgtgagtct
    10801 ttttgttctg cactatctat gaatgagata ttatgtgctt catttgactt agcaccagct
    10861 tcttctttgc cacatgcaga taataatagc gcaaaactaa aaatcatagg tattgttgca
    10921 ataactctta actttttcat ttataagcac ctctttttta tcggtctgat atataatttt
    10981 atattatcat atttttattc caatacacag atactcttat ataatgtctt ttcatgtagt
    11041 ttatcaaatt tctatttcaa accgcttaat acaaatatca aagcccaaaa ttttctactg
    11101 gaagctttta gagcctaaaa tatatttttt atttgatcca aaacttcagg gaaatacaaa
    11161 cctaatatca acacagcacc tataattaat agcggaatca ttttcttaat aaaagagatc
    11221 acagcaaaaa ctatcagtac tgcaactaat actaaagctg ttttgggatt agaatataaa
    11281 aactctttga ctgtagacgt tacatttaag tatagttcaa tcaatacgcg aaaatctcca
    11341 ttacttagtt tatccataac cgttctaaaa tcgatcatat catttcctca ctctttccaa
    11401 ttttattgct tcttttattg aagaagcaac ttgttcatta ctatgcttat ctatatataa
    11461 tggggaaaag taatttttat caaaaaagta atttgcaaaa gctacatcgt cttctgtctt
    11521 ccaattataa aaacagtaat tcccactaat cggtatttca aaaagccccc aattctctag
    11581 ctccatgttt tctttttcaa acaacactat gatttttttt acatcttcta tagtttccga
    11641 atcactgtta agtagctcac gtaaatatat attttttgta ttactgcaca ctatatcgaa
    11701 gtttttcata atttctattc tccgtttcta ctaattcatt cctatttcaa attcacgtgc
    11761 ttgtctcatt ttctcttgct ctatcattcg ttgcgctact tcataatcat ttttcgcacg
    11821 atacatattt aaatcatcat tgattactct atcaattttt ctaataagct ttttatcttt
    11881 aaatttttca gaaatcttct tgaagtggac ttcatttttt tcacgtgcta attgtcgttc
    11941 ttctaatctt ttttggaaat catcataact ttgtttagca tatttcgttt taaattctgg
    12001 tttttctcta tcaaacttaa cgtttaattt ttttccgtct aatagatttt taagttcatt
    12061 acttttcaaa tttttttctt cagattgctt taaaagtttg atttgtccta ctacagcatt
    12121 acccattcga gcattaaaat ctttttttct atttaaataa tattcttgtg gattgtagcg
    12181 tttaccgtag gttgtttgat atagctcatc aatttcttta gtcatacgat catattcttt
    12241 tctttttgga ttatcctttg tcatataatt tgtgacttca tcaatctttt cttttgtttc
    12301 ttcaggaagc tcattgtagc cacgtgttgt actaggtatt tcttttttta attcctttag
    12361 gtactttact aattctttgt catatcttga attattttct acatataatt gaatcaaaga
    12421 atcttttaca gactgtttaa gttctaaagg aacttctttt ctcaacttag aaattttttc
    12481 aaactcgttc gttctatcta atattttgct accgaattta aaaatcatat cgtctaacgt
    12541 agactgtttt ctcttacccc tagcttgtaa aacaccatca tattccatta tttcacgagt
    12601 atttttacgc tccatagctg ttacatgaat atggatatgt tcagtatttc tgtgaatcgt
    12661 tgcaaaccaa aaaggatcaa ctagttcttc cttttcttgc atttttccca tcatagactt
    12721 tgttgcttca tatagaacat tttcatttaa ttcatttgtt tctggattat aatatccttc
    12781 ccttactaaa aaatcattat cgaatgaaat tacatcttgg aacatcaccg atccattatt
    12841 ttttgcttct atgaccgatt cttttatttt tgaaatgtct tttttagtga tatatctttt
    12901 tgtattgcta aatgctccgt ttactatttc attatctaca ttttctaaga tagctttttt
    12961 acgtgtcata taatcaatat atttatccat ttcacgtaaa tctttaatca ctggttgatc
    13021 tgtttcctgt tctatttttt ccaaagcttt ttctattcta gctaattcat ttagttcatc
    13081 ttctgaataa tcatgatcta gtttatcttg cctaatagcc tcttgtctgg aaatataatc
    13141 tacaaatttt tgaagttcta ccatgtgatt attctttttg ctttttccag tttcaaattt
    13201 tgctttaaga ataatgctcg cactttttct gttaaagata tgttcagtca tatcatcacc
    13261 caccattttc aaaaattttt tctacaggcg gttttgtact taatgctaag ttgcattaga
    13321 attttttagt gttcaatccg ttgtaaatct taatgacggt gaactaaaca ttgttccctc
    13381 tcccaaacga ctactttctt gttctggttc agcacgctct tcttctaatt ctttactagc
    13441 taatccacta ttaattaata gtttatagac agactcttct atactattta attgttcact
    13501 ataatcttta tgtgtactgt cttctttttt attaaagttt tcaagttgct gtttttgcaa
    13561 ttctattatc gtttcaagtt cgctaatttt taattttgct tcttctaagt ttttagctaa
    13621 ctcttctttt tctggatcac ggaaattttg attggctttt gttaacattg gtaaatttga
    13681 aacaacataa cggaacatat tggattttgt ttgaaatact gacttgttta cttcaaagca
    13741 cttattgaat ttttctttta catcttctgt tgctgatact ttgaaataag ggtagttcat
    13801 ttatttaaat cactcctttc gctaattaaa aaattgaata agcgttcata aactgaagtt
    13861 aactcgctaa tactttctac catttgttca tttgacaata aaagtttttc aactactaaa
    13921 tcaaattctg actttttata gtcaatcaaa tcgatctcgc tatcttggcg aatgattgct
    13981 aaaaaatagt cattcctact aaactttttt ccggtacgtt cttgaatttt attaactttt
    14041 ttatcaatct cttttacata ttgtggatca attcctctga ttgaaacatc catacacaca
    14101 cctctttcaa gctgttttta tctatctggt atatattatt atatcaaagt ccaaaggaaa
    14161 attaaagatt taaagacaag accaaggaat taatacaaag agcataaggg aaaactgata
    14221 gcaccttcct aaaggaaggt ggctaagttg gctgtgccaa ctggttttct ttcaaaatag
    14281 cttcatattt tttgctatca caaaaaaatc cattttcgac ctattttcgg tcataatata
    14341 gtacctactt ttggtcatag tttcgtccgt agttttggtc atagtaacta aagcttttac
    14401 gcccaaaaaa agagtggcaa gttttacctt gccactctct tttctattca caaaattggt
    14461 ttaaattcgc tttttttagc acttcgttta tatcatagca atcataatat ttacacaaca
    14521 tcattcctaa ttttaacgct tgttcctctt tatatttttt aagctctgca ttttttttgc
    14581 gcttaatcgc ttttctcttt tctttatatt ccgcttcaat tctcattaat tcttcttcag
    14641 ctttctcttg aatagtgaca atagaattac acattctctc taaactccct taaaataatt
    14701 taaacacgat cactcaaatc ggcttgcaga cttttctagt tgctttattt tttcctcaaa
    14761 ttgtttgatt ttattttcac tttcctctat tgaagattta ttattttcaa tagtcgtctg
    14821 catagaactg atttcatttt ttgtatctgt ttgttcgtct agggtttgaa attctaagtt
    14881 ttgattagct ttatcaatat ctttttgcac tactttattt gctaaattca ttttttcaat
    14941 tgattttttc tcatctttta tagaattttc tatagcttta atctgatatt taaaattatc
    15001 tttttgcaaa tcagaagtac tttcaacttc caatttagca tttgatttaa tatctttgtc
    15061 tactgcatag aattttattt ccttttcttt taagtcattt gaaggttcta attcaggata
    15121 aatatatcta gggttaactg tcacagaaat tacaccaaaa tctttaggca gattttgata
    15181 ataaattacc gtgtattcat ctgacaattt tacaactttt gtcttaacat cttcattatt
    15241 tttgttcgtt ataaaattta atttatagtc acttttaata ttacttagtg atttagtacc
    15301 tgtatcatct tgataccgca atactaattt aaacagtccg gcttccctat ttatttccat
    15361 atcatcaaca tacatattca aagaagacgt tacattttgg gctgaactca tttttacagt
    15421 ttcaaaatca taacttttcc ccatcacata aggagaaaaa acgattatca ttcctattgc
    15481 taaaaatcca agtatagcac ctaaatataa ttttactcct ttcgtttttt tatatacttt
    15541 gtcaatttgt gccttatttt ttttatttgg ttccttctta ttatgaatgg aatatttctt
    15601 attcatatac ttcacctcac tattttaagt acttgatttc ataattatct accaacactt
    15661 ggccgtcttt attggttact tcaaaaagca tatagactgt attttctgta tctccactag
    15721 aagattctgt tttataattt acttcagcaa aataattaat agtattggcg ttaacataat
    15781 tcgtatataa ttttgaatct ttatatttac ttttgaatgt tggatcactt gaatatgctt
    15841 ctcctttttg agtagtagat ttactttcta aagtcttttt actcgttgta atcatgtcgt
    15901 ccggcacgat agaatcagct acagaaggag tagctatttt ctctaaatca gctttctttt
    15961 cttctacttt ttcaatatca tataccggat atagctcaac aaatttctta gcaacttcag
    16021 caatttgttt atttaattca ctgccatcat cattagttaa gttcgcttct acatttgcat
    16081 ttagatcact aatattttta cttaacttgc tattttgttc ttctagttgt gtaacttctt
    16141 gatccaattt tttatttttt gcttgaatgt tacttgtatg aagttgagat aacaatccac
    16201 ctaaaactaa aataataatt aaaacaccac aaatatattt ccatttatta tcattttttc
    16261 tcttaatcat ctattttctt cctcctattt tttgactcta cgaatctctg gactatgtga
    16321 ttgccaatat gaatcttgcc aattgtgata accaattccg cttgaattac tgtcatacat
    16381 agttgtttca ttgacataaa tacctacgtg agaaatgaaa tcaggcgctc catatgttcc
    16441 tctaaagaaa atcagatcac cagcttttgc ttctttaatt gatactgctt cagttgcttc
    16501 atactgcgtt actgtataac tatctattgt aattccggcg ctaaattgat aaagataagc
    16561 tgttaatccg ctacaatcaa aacctgtcgt tggattttta ccgccccaaa cataaggcca
    16621 accttgatat ttttccattt ctgttttcat ttttccccaa aattctgaag gattagccgg
    16681 attaccacta cccccaccga tatactgttt aactaactct gcatagtaaa aattaccacc
    16741 atttaaatac aaatatgttt ttccatattt aattgatact tcatttacat atgaataagt
    16801 ctgtcctgta gtatttccta aacttggagc aacaacattt ttagagtagg cttcagccgt
    16861 ttcgatagta tgtgtttttt tgttttttcc taagtagtgc atgtaagcta ttccgtaatt
    16921 gtaagcttga actattccat catagttact ttccatgcca taacttttag caatgttata
    16981 cacattactc aaatgtttta gcccttgatc gatactttct tcatattgaa gtgtgtttac
    17041 aggcaatccg gctgattcgc tactttgcat taaatccggc aatttacctc cggattcaca
    17101 ttgcataatt gctaaagcat aaggtactaa ttctttaatt ccgtattttt cagcatattt
    17161 ttctaccgtt ttttgccatt tttgaacttc aggtgacaac ggtttgttgc caccaatgac
    17221 ttctgcacct cctgtattag ttgcagtttg tgttccttgt aatgtagcta aagaacctat
    17281 aactaaaagg ccaaccaatg ttatcagtcc tataactacc cctattttta tttttaaagc
    17341 aagacctatt ttctttgata cttgcttcat cccttgacga gcgagtgttg aagcaacatt
    17401 attatttttg ttctgctcca ttaaattcac ctccgatttc tattattgac caccatcgaa
    17461 tcgttcaatt tgctctggat atggatcaaa attaatcata taggtgtctt cactggaagt
    17521 ttgaactaca gcttgtccta cttctaattc tggtaaaagc ataatttctg tatcagtaat
    17581 tgtatttcct aaaacttttt ttatgtcttc caacaatgca gaatctaaat taaagaagca
    17641 tttaaaagta catagattaa aaatgtttct aatcacatct gctacttctg atccagaatc
    17701 tttagggata atttcttcag gcgtttgagt tgcaaaataa acacctgcac gcatttttcg
    17761 catttctcgc tcaaaactta gtacatattc acaagcatac ttgttatttg aattgataaa
    17821 tactgatagg taagagtttg atattatctc cgtcttt
//
LOCUS       DACEND010000048        17585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEND010000048 DACEND010000000
VERSION     DACEND010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.049"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..1087
                     /locus_tag="IW746_001385"
     CDS             5..1087
                     /locus_tag="IW746_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1078292.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IW746_001386"
     CDS             complement(1305..2447)
                     /locus_tag="IW746_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1078293.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IW746_001387"
     CDS             complement(2452..2637)
                     /locus_tag="IW746_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1078294.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IW746_001388"
     CDS             complement(2991..3236)
                     /locus_tag="IW746_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078295.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IW746_001389"
     CDS             complement(3230..3631)
                     /locus_tag="IW746_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1078296.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="IW746_001390"
     CDS             complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="IW746_001390"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1078297.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5647..7413)
                     /locus_tag="IW746_001391"
     CDS             complement(5647..7413)
                     /locus_tag="IW746_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1078298.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7426..8220)
                     /locus_tag="IW746_001392"
     CDS             complement(7426..8220)
                     /locus_tag="IW746_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1078299.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(8230..9606)
                     /locus_tag="IW746_001393"
     CDS             complement(8230..9606)
                     /locus_tag="IW746_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078300.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLVLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(9726..10373)
                     /gene="eda"
                     /locus_tag="IW746_001394"
     CDS             complement(9726..10373)
                     /gene="eda"
                     /locus_tag="IW746_001394"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1078301.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10466..11893)
                     /gene="uxaC"
                     /locus_tag="IW746_001395"
     CDS             complement(10466..11893)
                     /gene="uxaC"
                     /locus_tag="IW746_001395"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1078302.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11967..13046)
                     /gene="uxuA"
                     /locus_tag="IW746_001396"
     CDS             complement(11967..13046)
                     /gene="uxuA"
                     /locus_tag="IW746_001396"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1078303.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13049..14680)
                     /locus_tag="IW746_001397"
     CDS             complement(13049..14680)
                     /locus_tag="IW746_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1078304.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14986..15657
                     /locus_tag="IW746_001398"
     CDS             14986..15657
                     /locus_tag="IW746_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078305.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKILEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(16360..16715)
                     /locus_tag="IW746_001399"
                     /pseudo
     CDS             complement(16360..16715)
                     /locus_tag="IW746_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16899..17306)
                     /locus_tag="IW746_001400"
     CDS             complement(16899..17306)
                     /locus_tag="IW746_001400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078306.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
BASE COUNT         5715 a         3393 c         2323 g         6154 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaaaa
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctaatttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gacaaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaaggag atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taactatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tggatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcatttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagagag cccctttttt tcgaaaaaac tagaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaacgat taggctaggc
     3781 taatcgtttt ttgaacattc ttaaaaaaat ccaaattatt ttttataact gaaattgggt
     3841 attgatgacc atcgtttctg tagatagtga gcaatcattt taggttttct atctctagta
     3901 aagattcctt tcttatttcc ttgcactctt aaaatccctt gactcgttgc aaaatctgca
     3961 aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct
     4021 tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca
     4081 gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca tctttctttc
     4141 catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca
     4201 taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaata
     4261 tctggcgttg attttagata agtaactatt gttacaggtc ttttctgcgg atcaagttct
     4321 ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt
     4381 tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc
     4441 ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc
     4501 acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca
     4561 tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa
     4621 tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt
     4681 acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta
     4741 aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata
     4801 ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact aattttcata
     4861 ctatataaat aagcatctag aggttcccaa aaaattggat tcttaagctg aatcattcct
     4921 ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt
     4981 tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc agttattctt
     5041 gtattaatag taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta
     5101 tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca
     5161 ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg
     5221 tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca
     5281 aatggtgtaa acccaccttt gtgttgagta atcaactttc cattcaaaaa aacttttgct
     5341 tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga
     5401 gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat
     5461 aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct
     5521 aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt
     5581 aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc
     5641 taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt
     5701 aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc
     5761 ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac
     5821 agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact
     5881 tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa
     5941 gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt
     6001 tttaattttt tcttcttgcg ataatccttt caactcttct tcaggtgaaa tgtatttttc
     6061 aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt
     6121 tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct
     6181 tttatatttt tctggttcaa taggccaaat atcttgttta tctttaacta atgtaattgc
     6241 ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc
     6301 ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt
     6361 taaccctaaa attcttttta aggcatctaa taaccgatca tcagagataa ttcctttacg
     6421 ataaccttcc atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc
     6481 acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc
     6541 taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa
     6601 gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg
     6661 attaaaatgt tttgaataag ctggcatatg gatatgacct gccataatcg aaggtaatcc
     6721 agcttctatt aaacctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa
     6781 actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg
     6841 tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc
     6901 taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt
     6961 tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat
     7021 cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac
     7081 ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc
     7141 agcaattaat aaaggaattt tgctttctgt ttgtaggata tagttttgat catatacctc
     7201 atcagcagtt cctgggttat atcgtacagc tccaatatgg tactgattga ccatattact
     7261 caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc
     7321 caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata
     7381 tggttttttt cgtaagtcta cttttatagt cattttcttt ctcctctact tattagtaag
     7441 atttttccaa aaagtatcta taaatttttg ttcaatatct acatactctc ccgataaaca
     7501 tttaaataaa acatcagttc ttaagttttc ccatgattct ttttctttat taaccaaaat
     7561 aggataaaag aaaactttat ttttatttgc agcttctaga tcaccaggag aatcaccaat
     7621 cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa tgtcttcttt
     7681 ttttccttta tcttgacaac acagttcatc aactaatgat aataattcat gtctttccca
     7741 ttcttcttga acagcttcct tattagctga gcttacaata attatcttcc catatttttt
     7801 cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt tagcttgact
     7861 aatctgttga ttgacttctt ccgaccattt taaagctttt aataaatcct ctgactgata
     7921 atattctatt tccctcttca aagatggatt agacaattct tttgttagag atacccagtg
     7981 ctttaatcga ttaatggatg ttacatcatt tttttctaat gttttaacta aacctaaaaa
     8041 tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt
     8101 ttctctatga tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga gattgtgttt
     8161 ataagtcatc gtatccataa cacagccatc tgaatcaata caaaatatcc aattattcat
     8221 atttctctcc tatttatatt tttgttttaa aagctcgcaa ttttctaaga ctttatcttt
     8281 agataatgga taaaggaaaa ataaaattaa tcctacaata aaaaaacaaa ttgatggaat
     8341 taatgttgaa gcgttatata acttttctaa aacatctccc gtttgtatat ttgctaaaga
     8401 gtcatagcct ataatagaaa gagtccaacc tccgacgcct ccagctagag cttgacctat
     8461 ttttctagca aaagaatata ccgcatatac ggtcccatct tctctttgat cagactttac
     8521 ttcttgatca tcaataacat cagtaatatt tgcccaaata acagtattaa acacacccaa
     8581 acccatatag ccaaccgaac taagaattaa gaatacatac atattactag tttttatcaa
     8641 aaataagatc aaatatacta ttgctgaaaa gaacattcct actgatgcca gttctttttt
     8701 tccatatttt ttacttaaaa acaaagttaa aggtgaaaca actattaaaa ccaaaatagg
     8761 attgataaaa ttcattaaaa ctattccctg tgcatcatta tagtaatcag gaaatacata
     8821 attattcata ctcataatta ataattgagc aaggactaag aaaatagccg caccaattat
     8881 agaaagtaat gccctatttt taactaaccc ttttaaagat tttaacaatg aaactttgaa
     8941 ttgagattgg tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat
     9001 aagtgcacat atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat
     9061 ttgagcgcct gaatcatctc ttgtataaat aaataatggt gttaatacac caatgatcaa
     9121 acttgctaaa gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat
     9181 agaagtaata gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact
     9241 cccccataat atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat
     9301 catagaagtt tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat
     9361 ccacggttta aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt
     9421 tacatcagta actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc
     9481 gcttatcccc aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt
     9541 aaaatcgttt ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct
     9601 ttccatacaa ttctctccct tcttttgtta agcgttttca ttcttatgaa agtacgtact
     9661 atactctgaa gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt
     9721 ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc tactcgactt
     9781 acttcttcaa aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca
     9841 acaactccta cttcaaacca atcttctaag ttctctagac ttactccgcc agtaggcatt
     9901 atatttacgt gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa
     9961 cctgggaata gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt
    10021 gtcatacaac ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga
    10081 tcaaaagatg gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac
    10141 gcatctaaaa ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca
    10201 atttctttga ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt
    10261 ccacccgcaa taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca
    10321 accgcaatca ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc
    10381 tcctctatct ccttctttta tggatacata ctttgtctag ctgtactaac ttaaaaagaa
    10441 taaatttaag cttttatttc aagtattatt ttctacttcc gatttcaaaa aaattaaaat
    10501 aattatacgc attacgataa caaatattct ccactatctg actaagatac atttcatcat
    10561 ctggaattcg gcctgcttta acccactcgc ttagtaattg acataatact cgacgaaaat
    10621 actcatggcg aggatagctt agaaaacttc gagaatcagt caacatacct gtaaaattcg
    10681 acaacaaact ttgttgcgcc atcgtagtca actgttgacg cattccttca gctgtatcat
    10741 tgaaccacca agcacatccc aattggattt tttgaatcat acccccttgg aagctttgca
    10801 ttgctgttgc tagtggaagc caatcagttg gatttaatga atataaaatt gtttttggta
    10861 agcacccact cttttcagcg gtatcgaaca atgctacaac attttgtgtc aaactagctt
    10921 gatcccccat cgaatcacca ccgacatcta ctccaatttg ttcctttaat atacttgaat
    10981 tatttcttaa agcatttaaa tgcatttgca tcgtccaacc atatcttgta tactcagaca
    11041 tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc tgataaatgg aaatccgttt
    11101 ttcctgctaa agcttttatt aacagattgt cgagtatttt ttcatctgct atttcataaa
    11161 aataagtatt caaaccgtga tctgctaatc gacagccaat ttgatgaaaa tgatccactc
    11221 gttggctcaa tgcttgcttc aacccattaa agtctttgat tggtatagcc gctgcttgac
    11281 taagttttaa aatatagttc gaaaagcctg atgatttgat actccagacc ttatctggac
    11341 ggaaagtagg taataccttg aaagaattat cctgtttaga aagctcagta tgatacccca
    11401 agtcatctgt aggatcatcc gttgtacaga tggctttcac atccatcatc tccaacaatt
    11461 ctttcgggcg ataggaatcc gtttggattt tttcgttagc acgtcgccaa atttctggtg
    11521 cattcttctc agaaattgtc aaatcaatat caaagtaacg ttgtaattcc aaatgagacc
    11581 attcaaaaat aggattacct ggcgccttct ccactgtttt tacaaaagct aaaaattttt
    11641 cataatcatc acctggacca ctgatcaact cttcttctat accattagct cgcatcaatc
    11701 gccatttata atgatctcca gctcctttat catttaacca tacctgagtc aaattcttgt
    11761 attgcttatt ttctagcaaa tatgctgggt ctaaatgaca atgatagtcg ataattggtt
    11821 ggtgttttgc aaatttatga aaaagtcttt tagctatttc attctctaat aaaaagttct
    11881 tatctaaaaa catatttaaa cactcctttc cttccgttag tttttagaag agaaaatttt
    11941 tcctctaaat aaactccatt atataattat ttactcgccc ccttttcaag cgcttcccaa
    12001 ataccattta aataaactgc acctagagct cggtcatata atccataacc aggtctaccg
    12061 gtttctcccc aaatcatccg accatgatct ggacgaatat agccattaaa tccattatcg
    12121 tacattgttt ttaatatctt atacatatct agagaaccat attctgattt atgcggcgct
    12181 tcgtaaaaat cgagtccatt gtcacttaaa tatttgatat tacgaacatg tgcaaaagga
    12241 atccgatccc gttgacaaaa ttcagataga atttcataga catcattatc tggtcgttct
    12301 gcaatacttc cagtacataa tgtaatacca ttagaaggag aatccactac attacacaac
    12361 caatctaaat cgtcacgatt tttaacaata cgaggtaatc cgaaaatgga atatggtgga
    12421 tcatctgggt gaactgccat tttaatccca acttcttcac atgtcgggat aattgctttg
    12481 agaaaatatg caaagttctc tcgtaatttc tcttcatcaa cttctttata ggcttctaat
    12541 aattgcttaa tttgtgataa ccgatgaggt tcccaacccg gcaattcaaa attattagta
    12601 gaattttcta cttttctaat aatttcctcc ggatcagtag ggatattttt tgaaatatag
    12661 gctaacgtag aagacccatc atctaaacga taatccaaat ccgattttac ccaatcaaat
    12721 actggcataa aattataaca gataacctca atcccaaatt ctgccaaatt gcgaatcgtc
    12781 tgtttataat tctcaatgta ttgatctcgt gtttgtaaac caattttgat atcatcatga
    12841 acattaacac tctcaataac tttcaaggta agtccattgg cttcgacttc atcttttaaa
    12901 gatttaattc gttctctcgg ccaaacctct ccggctggta tatccattaa tgttccaaca
    12961 attcctttca ttgctgggat ctgacgaatt tcttgtagtg agattttttc ttcatttgcc
    13021 ccataccatc taaaagtcat ttccatagtt actttcctcc aacttctgtt atatatttat
    13081 tgattgtttt acgaactgct tgaggaccta ctagcatttc gcaaaagatt ttttcagttt
    13141 tctttcctac cccaacttga tataaatcta aaccaaaaat ttctttattt tctaaaattt
    13201 cctgtaccgc acaatgaata tttgaaggat cttgttcacc taatttcaat actgataatt
    13261 tatcctgcaa ctctaccaat aacggatcag gacttggcac aaaattttcc ccttgatcat
    13321 cgactgccag taaataacgt aaccaagcag caatagcgat aggaataaat gttagatttt
    13381 ctgcatttcc gtctttttcg acatactttt taattgtttc accatagcga atcgctactt
    13441 tttgagaagt atctgttgca attcgttgtg gtgtatctgg aatataggga ttaggcaaac
    13501 gtttattaat tacttcatca ataaattgtt ttggatcaat aatgcctgga ttttcaacta
    13561 ctggtaaacc ttctaaataa cctattcctt tgattaaacc aaccaaatca tcatcctgca
    13621 tctctgctga aattgattga tatcctagca gacaacctgt tactgccaaa gcagtatgaa
    13681 gtggattcaa gcttgtcgta accttcatga tgtctgcttt atcaaccgtc tctctatctg
    13741 tcataatcac accagccttt tccaatttag gccggccatt tggaaaacta tcttcaataa
    13801 ctaaataatg aactacctca gtattagtga aagcagcaat attggtatgt ttttttgtgt
    13861 gaatgatatc taaatcctcg agccccatca tcaatagatc ttcctttaca ttctctgaag
    13921 gatttggcgt aattcgatca atcatactcc aaggaaaagt cactttttct ttatccttta
    13981 aataagcaat aaatttttca gaaacatatc cttctttttg ccaagcttct gcaatcgtaa
    14041 aaatactatc ttgaaaacgt tgaccattct gtgagaaatt atcagtagaa accatcgcaa
    14101 tcggcaactc tccagcatta aatctgctat ataacaagga tgttacaata ctcatcgtat
    14161 gttgaggatt gctaggtcca gatttgatat cagctttgac taccgaaaag aattgccctt
    14221 tagaatcttt taaactgtaa cctttttcag taatagtaaa cgttgtaaac tgtaatttag
    14281 gatcttcaaa atatttaacc actctttgat agtccgctat attcttcgga ttgcaatata
    14341 aactatcagc cgttactgcc aaaagccttt ttttgagttc accatctcca ctcataacaa
    14401 tttccaaaat gtcattgttg tagtcatgat aagctttttc cactacttct tcatcaaatg
    14461 tttcacacac aactactcct gtttctaatt cattttgatc tgctaagcat tgagcaattt
    14521 ctgcatgaaa tcctcgataa agatttcctc caccaaaatg aagccatact ggatgttgct
    14581 ggccttcttg cttaacttct ttgacatcaa aacacggcag tagaatctgc atttcattga
    14641 attcttcgat agaattttgc caatttttaa tcgaaaccat aattaacctc cttaaactaa
    14701 tatgttagtt ttgatacatt ttatatttta aaacccgttg taaccgttgt caatataaaa
    14761 atggaataaa cttgcttttt ttctaccata tcagaattat tgtatttttt attcagaaaa
    14821 ataaaatact ccttcttttt ggactgattt tatcgaaact tatttttttc tttttttcat
    14881 gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa
    14941 cagaattaaa atatcagcta aaataaaagt taaggagcat tgagtatgac aattaaaact
    15001 agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa
    15061 cctggaaaaa aagtatcaga aaaaatatta gaagaaacac tttctattgg tcgtacacca
    15121 atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc
    15181 ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa
    15241 catttggaac gacaaattat gttagagtgt tgtgccaaaa ttaataacca aaaaaaacgg
    15301 attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt
    15361 ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa
    15421 gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgttg gctacgagta
    15481 aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca
    15541 ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg
    15601 cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaag tatataactt
    15661 ttaactcttt ttaaattaaa tgattgttct gatcagaaaa atatcgcttg ttttcttatt
    15721 atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg
    15781 ataactaatt atgactgctg ataacttttg atattaaact agtatctcca atccaaataa
    15841 aaaatagata tcttgttcat caaaattagt tcatggatac ttaccttcat ttacatcatt
    15901 cagcggtttc tcattttctt ttattctatt atttcatttt ctaatagact tggcttcatt
    15961 tcgcaatact tttttacaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt
    16021 ttgtgacatg ataagtatgc tcctttttgt cttttccact aatacaacga tcaaagggtg
    16081 cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg
    16141 tttcaactac atcttatatt tcataataga ataaatagcc ttttgcatag ctgcttaact
    16201 tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat
    16261 tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta
    16321 ggattgatgc cgattactta tattctacgt atagttttag tattcttttt ttatcaccag
    16381 taaccaattg agaacagtcc taaagcagaa tagtctttta tctcaaaacg cttaatttca
    16441 ggcttaggaa tatgaataat cttttgagta tattctttct cttttaatct tactgaagaa
    16501 ataccactgc ctatcttttt tacaaatatt tcatttggtg tacagactaa taaaacaata
    16561 tttgtaatca atccttgcac taaagaatta aaaggaagac tatttaaaac agggtgtgca
    16621 taactataac atgtgaagta gttattttga actttaactt ttaaaaactt taaaaataat
    16681 cttatttgtt cttctaaaca atcatctata ctcatataat cactccacta attacttccc
    16741 ccaacaagaa atagcattaa tagctatacc agctgcaata gttgcagcat tgaattttgc
    16801 aaattgtgca ggagttaaac ttctttttgc tacatctaaa agtattttaa cagcataatc
    16861 ataatttttc ccttgcaacg cattaattaa agtttgcatt agattttcac gattaagtat
    16921 atcccaaaca acacaccaaa tggtaatgag ttcataacaa cacatgcagc atacttttga
    16981 agatcacgtt tttgacgctt tcctgttggc tgcacaaaat tttctaaaat aaatttccct
    17041 gctatacctt cagagccatc taattcagct tttttctgta tagcttctaa atcagtaact
    17101 atatattgat gtgtttctgt atcaacataa ccagcttctt ctaaatagaa tttcaaactc
    17161 tctactgcct gttgtctttg tgtttctata tcaggaactt cattcacttt ttcagcagca
    17221 taaacactgg ttccggcacc gactaacgta cttaatccaa ttaacattac acctaataat
    17281 gaaaaaaact taaaaaaacg actcattttt atccctcctg aaaagtattg ttgattatca
    17341 atttttatcc ttttaagctg ttcaataaga cgtttttacg ttccttccgt ttctatttta
    17401 ttctttctgt cttataacag cttttttcaa gtaaagaacg atataattct tcttaaaaaa
    17461 cagtctattt tttcggtata taataaaatt gtattgttac ctacatagcg aaacagtacc
    17521 aacatcattc aaatgaagat agcaaatttg cttcttcctt tttcttaagt gactatttaa
    17581 ataac
//
LOCUS       DACEND010000049        17136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEND010000049 DACEND010000000
VERSION     DACEND010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.050"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..2058)
                     /locus_tag="IW746_001401"
     CDS             complement(151..2058)
                     /locus_tag="IW746_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1078307.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IW746_001402"
     CDS             2188..3063
                     /locus_tag="IW746_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1078308.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IW746_001403"
     CDS             3143..4117
                     /locus_tag="IW746_001403"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1078309.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IW746_001404"
     CDS             4306..5442
                     /locus_tag="IW746_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1078310.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IW746_001405"
     CDS             5579..5923
                     /locus_tag="IW746_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1078311.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IW746_001406"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IW746_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1078312.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IW746_001407"
     CDS             complement(7618..8217)
                     /locus_tag="IW746_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1078313.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IW746_001408"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IW746_001408"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1078314.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IW746_001409"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IW746_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1078315.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IW746_001410"
     CDS             11423..12541
                     /locus_tag="IW746_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1078316.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IW746_001411"
     CDS             complement(12601..13893)
                     /locus_tag="IW746_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1078317.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="IW746_001412"
     CDS             14043..14603
                     /locus_tag="IW746_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078318.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IW746_001413"
     CDS             14732..15109
                     /locus_tag="IW746_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1078319.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IW746_001414"
     CDS             15042..17114
                     /locus_tag="IW746_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1078320.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5473 a         3104 c         3651 g         4908 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagtgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DACEND010000050        16942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEND010000050 DACEND010000000
VERSION     DACEND010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.051"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(122..808)
                     /locus_tag="IW746_001415"
     CDS             complement(122..808)
                     /locus_tag="IW746_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078321.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(944..1138)
                     /locus_tag="IW746_001416"
     CDS             complement(944..1138)
                     /locus_tag="IW746_001416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078322.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1188..1628)
                     /locus_tag="IW746_001417"
     CDS             complement(1188..1628)
                     /locus_tag="IW746_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1078323.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1632..2477)
                     /locus_tag="IW746_001418"
     CDS             complement(1632..2477)
                     /locus_tag="IW746_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1078324.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2626..4044
                     /gene="pepV"
                     /locus_tag="IW746_001419"
     CDS             2626..4044
                     /gene="pepV"
                     /locus_tag="IW746_001419"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1078325.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4101..5048)
                     /locus_tag="IW746_001420"
     CDS             complement(4101..5048)
                     /locus_tag="IW746_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1078326.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5271..5621)
                     /locus_tag="IW746_001421"
     CDS             complement(5271..5621)
                     /locus_tag="IW746_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1078327.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5821..6900
                     /gene="pepA"
                     /locus_tag="IW746_001422"
     CDS             5821..6900
                     /gene="pepA"
                     /locus_tag="IW746_001422"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1078328.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7044..7364
                     /locus_tag="IW746_001423"
     CDS             7044..7364
                     /locus_tag="IW746_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1078329.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7386..7850
                     /locus_tag="IW746_001424"
     CDS             7386..7850
                     /locus_tag="IW746_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1078330.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8055..8660
                     /locus_tag="IW746_001425"
     CDS             8055..8660
                     /locus_tag="IW746_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1078331.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8699..9988)
                     /locus_tag="IW746_001426"
     CDS             complement(8699..9988)
                     /locus_tag="IW746_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1078332.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10416..10895
                     /gene="rlmH"
                     /locus_tag="IW746_001427"
     CDS             10416..10895
                     /gene="rlmH"
                     /locus_tag="IW746_001427"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1078333.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10980..11489
                     /locus_tag="IW746_001428"
     CDS             10980..11489
                     /locus_tag="IW746_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1078334.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            11589..12239
                     /locus_tag="IW746_001429"
     CDS             11589..12239
                     /locus_tag="IW746_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078335.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            12690..13628
                     /locus_tag="IW746_001430"
     CDS             12690..13628
                     /locus_tag="IW746_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1078336.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            13641..14693
                     /locus_tag="IW746_001431"
     CDS             13641..14693
                     /locus_tag="IW746_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078337.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            14959..15594
                     /locus_tag="IW746_001432"
     CDS             14959..15594
                     /locus_tag="IW746_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1078338.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            15785..16576
                     /gene="msrA"
                     /locus_tag="IW746_001433"
     CDS             15785..16576
                     /gene="msrA"
                     /locus_tag="IW746_001433"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1078339.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         5380 a         2955 c         3419 g         5188 t
ORIGIN      
        1 aataaacggt gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg
       61 aggaactatt ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct
      121 cttatttgat gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt
      181 attcttgctc ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa
      241 agttatgatc aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt
      301 ctaattctga aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt
      361 ctagccaatc ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag
      421 tatccaaatc aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta
      481 gtattttttc tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga
      541 tcggtttttc caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt
      601 ttgaaacata agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga
      661 tcagtgcttc tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg
      721 agattttact tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag
      781 cttgctgttg caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat
      841 tataacataa atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc
      901 tgataggcat gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct
      961 cttgtatcgg tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt
     1021 ctttgaaaat gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga
     1081 tgtaaccgtg cattacttta acgacataca tggggatcac ctgctttttt cttttcatta
     1141 tacgaactac tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca
     1201 tcatccaaag aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc
     1261 ttcactaatc gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt
     1321 aattttttgt cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac
     1381 ttttggatag ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg
     1441 caatttactt ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac
     1501 cctagatgta ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga
     1561 tgaataatcc ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat
     1621 ttctccatga ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc
     1681 ggaagtgctt cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct
     1741 ccaatgaaac tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg
     1801 gctaaaaatc ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca
     1861 gcagaaccta aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt
     1921 ttcaaaacga gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt
     1981 tcttgtatcg gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt
     2041 tgttcgggat agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca
     2101 atcaccagcc attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc
     2161 ttagaagaag tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa
     2221 agaaactggt cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga
     2281 gcactccgat ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca
     2341 gctaaagcac tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata
     2401 cgtccaaatg ttcctttatg tgaaattttt ggacgaggtg tgatgattct tttcactagt
     2461 tgttcgttta tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag
     2521 agaaaaacag ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg
     2581 tggtatcatg aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg
     2641 caaaaagaag tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt
     2701 gtcaacagcg agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct
     2761 cgcgacgcat tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat
     2821 gtcgataact atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt
     2881 ggacacatgg atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta
     2941 atcaaggatg gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca
     3001 gcttactatg cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc
     3061 ttcgtagtcg gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat
     3121 gaagaagaac cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa
     3181 aaaggaaatg tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg
     3241 aaatcttttg tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt
     3301 caagtaccaa gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa
     3361 gcaaatccag taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt
     3421 ggtaaaggcg cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca
     3481 ttgttcttag ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct
     3541 gcttatgtcc atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa
     3601 atgggcaaat tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa
     3661 gcgaacttca tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa
     3721 agtgacatcg aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta
     3781 atggaaccac attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat
     3841 gaagaccaca ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt
     3901 ttgttgaaac gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat
     3961 caagcaaacg aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat
     4021 gccatctatc gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt
     4081 ttgcagtctt gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga
     4141 ttcaattcat attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact
     4201 aatgaaagaa gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta
     4261 ataaagcttg tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa
     4321 aagaagaaga tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta
     4381 ataatcatca tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct
     4441 tcaggacgat cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca
     4501 tggatcgtca attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca
     4561 aagatacgat tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt
     4621 ctagtaattg tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca
     4681 tcaaagtagg ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt
     4741 aatttctcga gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt
     4801 aaataatagt cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga
     4861 cgaggttctt tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc
     4921 gaattattat catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg
     4981 actgttgaaa ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt
     5041 tgttccattt ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat
     5101 aatcaaagga aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg
     5161 aatatatgtt tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac
     5221 ctctcttttg aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt
     5281 gcaccttttt ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca
     5341 taataaacag tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga
     5401 acggcgaatt tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct
     5461 tcttttagaa aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg
     5521 cgatgatacc aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct
     5581 aacccgcctt taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt
     5641 ctcacttgtt actccttgtg cttattttac cacactctca tattttcttt gaaataaata
     5701 accgctacca gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt
     5761 ttatccgcag atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat
     5821 atggaagaaa aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt
     5881 tttgaacaag atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta
     5941 cagttagatg gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc
     6001 cgtgtaatgg tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa
     6061 aacgggcttt ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag
     6121 cgttttacac ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc
     6181 ccacatttat tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc
     6241 gatgctgggt ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca
     6301 atcgttcctt acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca
     6361 tgggacaacc gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa
     6421 acattaggtc atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt
     6481 tctaaagctt ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca
     6541 gcagatgata cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg
     6601 cgtatccaag atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc
     6661 gctgaaacaa acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt
     6721 gctgctcata cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat
     6781 atccatacgc atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg
     6841 attcaaacac tgaaaggttt ggataaatca acagtgaata cgatcgtata cggaaaataa
     6901 tgcgatagtg aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa
     6961 aaagaggatc aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat
     7021 aaacagaaag tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct
     7081 acgtagaaac tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt
     7141 ttatcaaacc gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag
     7201 tagaccgtga caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt
     7261 ttgtcgtgat ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa
     7321 aacaggaaat cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg
     7381 tcaagatgaa tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac
     7441 ttgctgcttt caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag
     7501 caaacgtaat cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc
     7561 tagtcgatca gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga
     7621 aatctgttga ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca
     7681 tggcagtcaa attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc
     7741 taaatgcagt cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac
     7801 cttgtgatgt cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa
     7861 cttgtacgac ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg
     7921 tcacagtccc ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca
     7981 tgaaaaaaat ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata
     8041 atggaggaaa gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg
     8101 ttattatagc cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc
     8161 ggatcaaaga tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc
     8221 taacaatcga agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc
     8281 gattgatcaa agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg
     8341 ttgtcgggta cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg
     8401 agacagaagt agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag
     8461 ctggacaaaa agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct
     8521 ggccaggaac tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc
     8581 atttgccaaa tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg
     8641 gagaagcatt tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt
     8701 aattttcact attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag
     8761 ttgttttttc tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct
     8821 tatacaaagc agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa
     8881 tgacgtcgta ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca
     8941 cgatgacacc gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag
     9001 aaacggcaga taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt
     9061 tttccagttg attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt
     9121 ctacgcttac attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga
     9181 taacttgtcc ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat
     9241 ctgtttggat cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc
     9301 ggttcaagga agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg
     9361 aaccaatcgc aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa
     9421 ctgtttcgac tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc
     9481 caactaattc agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc
     9541 catctactac gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca
     9601 cgccgcttcc ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt
     9661 gctgcccgaa tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag
     9721 aaacgacagc gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt
     9781 tgatattttc cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac
     9841 tattgttgcc agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga
     9901 tccctccaac aagccccaga cctaacttac gccagaggct attattttta ctttttttca
     9961 tctttggtgt cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta
    10021 ctgttagtat agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga
    10081 aaaactttag gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga
    10141 tactgtgtaa aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc
    10201 tgtgtataaa gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat
    10261 aaggtaaaat aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg
    10321 gaaatctttt taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata
    10381 aacaaagtga gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag
    10441 gaaaattaaa agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg
    10501 cctatgcaaa aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg
    10561 aagcgcaaat gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag
    10621 aacaagaata cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg
    10681 ctaaacaaat cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg
    10741 gctctttagg actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa
    10801 aaatgacgta tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct
    10861 tccggatcaa tgcgggggcg ccttatcata agtgatgcgg tttttatcat tgatcctaaa
    10921 aatcttagca agacaaaaag tacgtaagaa agtaaagact aaatacttcg gaggaacaaa
    10981 tgaataacaa tgataggtta ttgcgattgc gttatgcgat tgatttgaaa gatacggatt
    11041 tgattaaagc ttttgagcta ggcggtgtac cactgactaa agaagaagct caggcggtac
    11101 tgaccaaagt acaggataag aacaaagata atgcagaaaa taatgtgtat gaaaaaacca
    11161 tcaacaacca agtttttgac gcattcctaa atggcctgat tctcttggcg cgtggtccgc
    11221 aaaaagataa gccgatagta gacacgccgc aaaaaagcaa ggaaatcaaa tatatcaaca
    11281 atgtgttgct aaaaaaatta aaaatcgcct tgtctatgac cactgatgat attttagacg
    11341 tttttgctga ggcagaaatc tatccatcaa aaggcgaaat cggggcattt ttgagaaaag
    11401 aaggccaacg aaattttaag ccttgcggag ataaatatat gcgtaatttt ctcaaggggt
    11461 taggaattta taatcggaga aaagtataaa gaaatttcat gagaaatcaa aacaatggtt
    11521 gttttgatga gagctagttt ttgtatgaga ttaaaatatt atcttaatcg aggagctgga
    11581 ttggttttat gaatgatgaa tataagaatg atgaagataa gatgcttttt gaagagatag
    11641 agaatcgatg taggctcaat tttgagcttc ggggaaagat gtctttaata caacaaaaaa
    11701 agtatttagc gaataaatca gagtttactc tgggacatgt tgaaaaatta atctcggatt
    11761 ggattagtag tagaagcgaa tttacaaaaa taaaacagcc gattaaattt gacatgaaaa
    11821 aacttttgtt aaataagagt gagataggta atagagatca atatattaga gctaaagggc
    11881 aagaaataat tgattcatta ggtgaaatga ggtcatacaa ttatttgtat gtaacacata
    11941 gagctgatgg aatggtcatt actgtgggga aatcttcttc taatgatatt tttttagatg
    12001 gagatctttt ttatcaactc aatacaaatc acttgtctgg cactgaaaat ataattttaa
    12061 ggacggaata tggcaatgaa atatttgcaa aatatgatga gattttaaaa aattatttgg
    12121 actgggcttg gataattccg gttgagtcag gagatgctaa gaaactggag agattattgg
    12181 gcgatgaact aattaataaa aaagtaccaa ttttaaatta ttattcccat agacaataaa
    12241 aggctatttt tcatgataat actagttata actgtaaaaa tggaagaaaa tctgggaaag
    12301 aagtttttaa ataagtgtat tgttcgattt taataaataa taagcaatac catccgtctg
    12361 tgttgcgcaa catcgtacta caatgaatat tttttcacaa aaagaaaatc atccatcgaa
    12421 tgaatggaag gttttagtat acacgaggtt aatgccccct cttttataat cagaggagtg
    12481 aggttttttc tatgaatgtt tctattttct tattgctagt ctagtttctc ttacgatgaa
    12541 attgggattc tattccagtt tttattgatt tgctgataaa ataacttgaa tggtggatat
    12601 aaacatgata aataagagaa gaataacaaa acaaggttgt gaaatgattc tcaaatgaat
    12661 ataatagaaa gaaaaaggag gaagaatcaa tgaaaatagc aattgcaggc gctggcgcaa
    12721 tgggaagtcg ctttgggtta atgcttcacc aaagtgggaa cgaagtatta ttaatcgatg
    12781 gctgggcaga acatgtccaa caaatcaaag agcatggatt acaagcaaat tttaatggta
    12841 aagaggtaga agcaaaacta ccaatcgttc ttcaatccga agtagaaaaa gaagatcaag
    12901 ttgatctgat tattctattt accaaagcga tgcagctgga aaaaatgctg caggatatcc
    12961 aatcattaat caaaaaagat acagaggtct tatgtctatt aaatggtatc gggcatgaag
    13021 atattattga gaaatttgta ccaatggaaa atatctatat tggaaatacc atgtggacgg
    13081 caggtcttga aggtcctggt caggtcaaat tatttggaag tggttcagta gaattacaaa
    13141 atttaggtga tggaaaagaa gcagctgcaa aaaaattagc agacaaattg tctgaatcag
    13201 gattaaatgc tcatttttct gacaatattc actattctat ttatcgcaaa gcatgtgtga
    13261 acggaacaat gaacggacta tgcacaatct tagacgttaa tatggctgag ctaggaaaaa
    13321 catcgactgc tcataaaatg gtggcgacga ttgtcaatga gtttgccaaa gtagcagcag
    13381 tagagaagat tgaactagat gtcccagaag tcattgcaca ttgtgaatct tgttttgacc
    13441 cggaaacaat tgggttacat tatccttcaa tgtatcaaga cttgattaag aaccatcgat
    13501 taacagaaat cgattatatc aatggcgcaa tttctagaaa agggaagaaa tatggtgttg
    13561 cgacgcctta ttgtgatttc ttgactgagc ttgtccatgc aaaagaagat agtttgaacg
    13621 taaaataaag gagagattga atggaaactt cacaaaaaat atcaccgaaa atttttctga
    13681 ataaagtcct cgctggtaca gcaacaggaa tcattattgg attgattcct aatgcagtat
    13741 taggtgccat tttaaaatat tttagtgcgt atccctttgc ggtaacgatc gcacagttag
    13801 cagttatttt tcaactagca acacctttga tcattggtgg tttgattgcc ttacaatttg
    13861 gatttgatcc gatgagaatg atggttgtag ccggagcttc ttttgttggt tctggtgtag
    13921 tcaaattcac acctgatttg ggtgaaacgg gtgtttacgt tggtgctggt acaggcgata
    13981 ttattaatac gatgctgaca gcatcgattg ctgtcgggct gattttactg attggaaata
    14041 gttttgggtc agtgtcgatt gtgttaacac cgattgttgt aggtgtaggt gcaggtttac
    14101 ttggttttta tttattccct tttgtaacag caattacttc tgcaattgga tcattgatta
    14161 ataacttcac taccttacaa ccgctgttaa tgtctatctt gattggttgt tcctttgcct
    14221 ttttgattat ctctcctatt tcaacagtag caatcggaat ggcaatccaa ctagatggtg
    14281 tttctgctgg tgcggctgcg atgggcgtag ctgcaaccac ttttgtttta gtagtgaact
    14341 cttggaaggt taataagtca ggtgtgacaa ttgcgattgc tttaggcgct atgaagatga
    14401 tgatgcctaa tctatttaga aaacctgtga ttcttattcc ttgtttagtt agtgcagtga
    14461 ttacagctat tcctgtcgct ctattttcta tttcaggtac gcctgcttct gctggtttcg
    14521 gcgtggtcgg acttgttggt ccgttggctt ctttagatgc agggctaaat ggctttcttg
    14581 ttgttctttg ttggttagtc atccctgttt ttgcggcact aattactcaa tttttatgcg
    14641 agaaagtatt taaactgtat gatcgagaag aagtcttcaa atttttaggc tagtagattt
    14701 tactaagaaa ttttcaatca atggatgcgt aagtgagatg tcaacaacat ctgacttacg
    14761 tttttttgcc tttttgagta ggagattgat gctgacaact gaactcttca gtgccattcg
    14821 attttttaaa atgtagaaga aactcacctt tatgttattg attgttcaaa aggcaactag
    14881 aataaaaata tgtgttaaaa tgagaaaaaa tttgattgtt ggatgtatcg aataatcact
    14941 aaaaagtagg agaagaaaat gatttttttg caaaaatatg gtttttattt tttattatta
    15001 ggtgtgatta gtgatttatc gacaccgtat atccttggcc ttttttatcc caaactgaat
    15061 caaatgacta ccgtaattag tgtgtttggt gacgtagata gtccagtcag acgtgctttc
    15121 ttggtctggt cagttgtatc tggacttttt tttgttcttt cgttgcctgc gttgtatcac
    15181 ctatttattg gaacatcaaa aacacttgca atacttgctg ttgcaacagt tggtttatat
    15241 gggataggtg attgtatttt tactgggtta ttcagtatca atacgaatga aagctcttgg
    15301 aatctatcga cttggatcca taatataggt tctgggctag gctatgcagg ctttctactt
    15361 ttcccattgt tacttgttct gctttatcgt caaagtggtc aaggtacgct tagccatttt
    15421 tatcttgttt taacagttat tagtttacta attgcaggtc tttacagatt agcacggatt
    15481 ccgtcgatta gtcagtttgc cttctttaag caattgggct tctttcaacg cttaagtttt
    15541 ttctttaact atttgccgat gatttgttta agtttttttc aactacgaaa gtagaaaaag
    15601 cagtgtgaag ctgattaagt aaaagaattt ctctatgggt aaccattcgt gtggatgtgt
    15661 tcgactaaga atagagatga ggagagcgaa aagcaagtta gtcttaactt gttatgcgtt
    15721 gtgctaaatt tcaggtagtc attttttgaa tgaataagaa gaaaacttga aagagggtga
    15781 cttcatgtta gatgtggagc agatggaaat agacctttat aatattattt taaatcgaaa
    15841 tacaagagaa tgggaaagaa cacaatttga agaggcaaaa gaagacctag caaaaatgtc
    15901 gataaagcag gtgatagaaa aactagaggt caacttgcgt ccgctagcac tacgagataa
    15961 tttgacacca gatgcgatgg atttttattt aagaatcaca ggggagtcag cgagtgcaaa
    16021 ggcttatgat tatgccaaac ataagataac ttcaccctta taccaagaac gggcgatttt
    16081 tgccggtggc tgtttctggt gtatggttga gccttttgaa acaaaaccag gtgtaatctc
    16141 agtgttatct ggttatacag gtggagagac caaaaaacca aattacgatc aagtcagtgg
    16201 tggatatact gggcatgttg aagctgttga gatcatattt gatacacgtc aaataaccta
    16261 tgaagagctg ttggagattt attggcaaat ttcagaccct acagatgcct ttggtcaatt
    16321 tcaagatcgg gggaaacaat atcgttcaat tctatttgtt gttaatgaaa agcaaaggca
    16381 attggctgaa aaatcaagag aacaactgat tcaatctaac cgatttagcc gtcctatcgt
    16441 tacagagatt cgccaagcaa agactttttg gccagctgaa aatcatcatc aacaatttta
    16501 tcagaagcaa cctaaaagat ataaaaagat caagcattcg catcagcttc atttattttt
    16561 tcagcgacat agatagagga aggttgtggc gcaaagcgtt cagctccgac aaaataatga
    16621 gcaactgaca aaaatagctt ttcatatttt tggacagtta tgaattattt cgaggagttg
    16681 ctttgagaac accgaggaaa aggttgtggc gcaaagcatt cagcttcaag tcgcaaggaa
    16741 acaggaggta tgacaagtct tttgtcacaa ctcctgtttt tttactagcc tttatttcca
    16801 tgtgaaagag aattcgaatt gtagaatctt gatttttcta tggaattttg tagagaatca
    16861 tcttgataat taatagacac ctaatcgaat tccacattaa tgatgaacta aattccgtat
    16921 aaaagcgcaa acaataacta ta
//
LOCUS       DACEND010000051        16818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEND010000051 DACEND010000000
VERSION     DACEND010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.052"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..454)
                     /locus_tag="IW746_001434"
     CDS             complement(<1..454)
                     /locus_tag="IW746_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1078340.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="IW746_001435"
     CDS             complement(559..1422)
                     /locus_tag="IW746_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1078341.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="IW746_001436"
     CDS             complement(1618..3717)
                     /locus_tag="IW746_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1078342.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IW746_001437"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IW746_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1078343.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="IW746_001438"
     CDS             complement(5070..6911)
                     /locus_tag="IW746_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1078344.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9486)
                     /locus_tag="IW746_001439"
     CDS             complement(7093..9486)
                     /locus_tag="IW746_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1078345.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="IW746_001440"
     CDS             complement(9593..10453)
                     /locus_tag="IW746_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1078346.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="IW746_001441"
     CDS             complement(10512..11033)
                     /locus_tag="IW746_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1078347.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11353..12072
                     /locus_tag="IW746_001442"
     CDS             11353..12072
                     /locus_tag="IW746_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1078348.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="IW746_001443"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="IW746_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1078349.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IW746_001444"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IW746_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1078350.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="IW746_001445"
     CDS             16432..16788
                     /locus_tag="IW746_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1078351.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5765 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaa
//
LOCUS       DACEND010000052        16719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEND010000052 DACEND010000000
VERSION     DACEND010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.053"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            169..1275
                     /locus_tag="IW746_001446"
     CDS             169..1275
                     /locus_tag="IW746_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1078352.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            1292..2320
                     /locus_tag="IW746_001447"
     CDS             1292..2320
                     /locus_tag="IW746_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1078353.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            2326..2805
                     /locus_tag="IW746_001448"
     CDS             2326..2805
                     /locus_tag="IW746_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1078354.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2821..4116
                     /locus_tag="IW746_001449"
     CDS             2821..4116
                     /locus_tag="IW746_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1078355.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            4127..4951
                     /locus_tag="IW746_001450"
     CDS             4127..4951
                     /locus_tag="IW746_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1078356.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            5116..6015
                     /locus_tag="IW746_001451"
     CDS             5116..6015
                     /locus_tag="IW746_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078357.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(6141..7010)
                     /locus_tag="IW746_001452"
     CDS             complement(6141..7010)
                     /locus_tag="IW746_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078358.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            7163..8623
                     /gene="rhaB"
                     /locus_tag="IW746_001453"
     CDS             7163..8623
                     /gene="rhaB"
                     /locus_tag="IW746_001453"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1078359.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            8620..9876
                     /gene="rhaA"
                     /locus_tag="IW746_001454"
     CDS             8620..9876
                     /gene="rhaA"
                     /locus_tag="IW746_001454"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1078360.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            9890..10735
                     /gene="rhaD"
                     /locus_tag="IW746_001455"
     CDS             9890..10735
                     /gene="rhaD"
                     /locus_tag="IW746_001455"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1078361.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            10737..11048
                     /gene="rhaM"
                     /locus_tag="IW746_001456"
     CDS             10737..11048
                     /gene="rhaM"
                     /locus_tag="IW746_001456"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1078362.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            11130..12281
                     /gene="fucO"
                     /locus_tag="IW746_001457"
     CDS             11130..12281
                     /gene="fucO"
                     /locus_tag="IW746_001457"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1078363.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            12408..13166
                     /locus_tag="IW746_001458"
     CDS             12408..13166
                     /locus_tag="IW746_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1078364.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            13266..13910
                     /locus_tag="IW746_001459"
     CDS             13266..13910
                     /locus_tag="IW746_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1078365.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            13917..14924
                     /locus_tag="IW746_001460"
     CDS             13917..14924
                     /locus_tag="IW746_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1078366.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            14940..15782
                     /gene="kduI"
                     /locus_tag="IW746_001461"
     CDS             14940..15782
                     /gene="kduI"
                     /locus_tag="IW746_001461"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1078367.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            15802..16605
                     /locus_tag="IW746_001462"
     CDS             15802..16605
                     /locus_tag="IW746_001462"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1078368.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT         5390 a         2868 c         3801 g         4660 t
ORIGIN      
        1 tttctccaag ttcttttttt aatttccaag taactgaaag ttacttttgt tatttaacat
       61 ttaggtcaat tttagacagt ttttgttcaa aaagcgatat ttatagtgga cggtattact
      121 gctatattaa ggatataaaa tgaaagcgtt taaaaaagtg gcgtaaagat gattattaaa
      181 gaagagattt ttgtacctaa agaattagtg aaagaaaaaa ttgacctgct cgttgaaaac
      241 ctgacaacga tcaaagacga taatggtgaa tttttgttgg actttgatgg actgaaagta
      301 gatgacaaaa gctggacagt gtggaattgg ccacaaggtg tcggtctata tggtatttat
      361 aaaaattacc agatgacaaa aaatcctcgc gcttatcaag tggtcaatga atggtttgag
      421 gatcgtatgg aagaaggcgc tccgcccaaa aacgtcaata caatggcacc gctattgact
      481 atggcttact tgtatgaaga cacgaaagat tcacgctatt tgccttattt agaacaatgg
      541 gcagaatggg tcatgaatga catgcctcgg acaaatgaag acgggttaca gcatgccaca
      601 tatggaccgg aaaataagaa tcagttgtgg gacgatacat tgatgatgac agtgcttcct
      661 ttggcaaaaa tcgggaaact gttgaatcgc ccagaatatt tagaagaagc tcgttaccag
      721 tttatgatcc atatcaaata cttgatggat aaacgaacag gattgtggta tcacggctgg
      781 acgtttgaag gaaatcataa ctatgccgaa gcattttggg ctcgtggaaa ctgctggttg
      841 acgattgcta ttccagagat cattgaaatc ttagaattat cgaaagaaga tgcattgcgc
      901 aaattattga tcgaaacact tgaagcacaa gtgcgtgcat taaaaactta tcagtctgaa
      961 agcggtttat ggcatacgtt gttggatgat ccttcttctt acgtagaatc ttctgcaact
     1021 gcaggatttg cttacggtat tttgaaagcg gtccataaac gttatttacc acaagaatat
     1081 aaagaagtcg cttatcgtgc tatccaagga ttgcttgaac aaatcgatga aaaaggagaa
     1141 gttcaaaacg tttcaatcgg gacaggaatg ggggacagtt tagattttta tcggaatatc
     1201 ggaatcacag caatgcctta tggacagtcg ttaacggtgc tttgtttgac agaattgctc
     1261 gtctcttatt gttaaaagaa aggaaaccca tatgaaaaaa acatcactgc tgtttgcagc
     1321 aagttcgctt agcctaggtc tgctaggtct aagcggctgc ggaaataatt ctagtgtggc
     1381 aactgtcgca gatgcagatg taaatgagat agttaccttg cgctttgctt atgcaagcaa
     1441 cagccagcca gtaattgatg caatgaatga atttgggcgt ttagtaaaag aaaaaacaga
     1501 tggttccgta cagatccagt atttcccaga tggacaactg ggtggagaac gtgaactgat
     1561 cgaattgaca cagtcaggag cagtagattt cacaaaagta agtgcctcag cgttggaaag
     1621 tttctctaaa gattattcga ttttttcagt tccttatcta ttcaatgatg aagaacattt
     1681 ctttaaggtg atggataatg aagaaataat ggagccaatc taccaatcga ccaaaaattt
     1741 aggatttacc ggattgacct attatgattc tggtcagcgc agcttttata tggtagacgg
     1801 tccgatccat acgccggaag atttgaaggg aaagaagatc cgtgtgatgc aaagcgaaac
     1861 cgctatcaaa atggtagagc ttttaggcgg ttcgcccgta cctatgggaa gtgatgaagt
     1921 gtacacgtct cttcaatcca atttgatcaa cggatcagaa aacaatgaat ttgtcttgta
     1981 tacagctgga catggtggtg tggctaaata ctattcctat gatgagcata cacgtgtgcc
     2041 agatatcatc atcatgaatg atgccatcaa agaacagcta actgatgagc aggaaaaagc
     2101 cattgaagaa gcagcgaaag aatcaacggt ttttgaaatt gaagcttttg caaaagcaat
     2161 tgaagaagaa aaagctttag cacaagaaga acatggagtc caattcaatg atgtagataa
     2221 tacaccgttt cgagaggcag ttgccccact tcatgatata ttcaaaaacg atcctaccta
     2281 caaagacctt tatcaaaaga tccaagagca aggagcgtag ctgtgatgaa aatgatttta
     2341 aacaaggtat tagagatcct gggagctgtt ttgcttgttg ggatggtcgc tgttgtttta
     2401 tggcaagtat tttccagaac ggttttagga aatccaaata cggtcacgga agagcttgtt
     2461 cgattcggtc ttgtctggtt tgccatgttg tcttccgcgt atgttgtggg acaaaaagca
     2521 catttagccg taacggtttt aagtgaaaaa ttaactggca aaaaaagtaa aattttagaa
     2581 gttgttgttc aaagtttatt tctggtattt gctgcgatga tcatggtgta tggcggatgg
     2641 aatgctgtca cattgacaat ggggcagatc tcgccttctt taagtattcc tatgggatat
     2701 gtttatctgt cagtacctgt atcgggggta ctcatcatta tctatagcct gatcaatcta
     2761 atcgatacat taagcaacaa gccagcaatt caagaaaaaa tataaaaatg gaggaataaa
     2821 atggcaatag aagccggatt agtgttattt tttgtatttg ttgtgttgct tgtattaggg
     2881 atgccgattg ctataagtat tgcattctca tcgatgatga ctttattgtt agttataccg
     2941 tttgatgttt ctgcttttag ttcagctcaa aaaatggtgg gaagtatcaa tagtttttcg
     3001 ctgatcgcta ttccgttttt cgttctctcg ggcattatca tgaataatgg agggatagct
     3061 aagaaactag tggattttgc gatgttgttc gtcgggcgcg tgccgggggc tttggctcat
     3121 acgaatgtgt tagggaatgc tttatttggt tccatctcta gctcggccat tgccgcttct
     3181 accgcgattg gcggagtgtt gatccctcag caagtggaag aaggatatga ccggaagttt
     3241 gcgacagcgg tcaatatcgc gtcagctccg acaggtatgg tcattcctcc aagtactgct
     3301 tttatcatgt attcacttgt tgctggtggt gcttcgatct ctgctttgtt tatgggaggt
     3361 tatctcgtag gcgctttgtg ggcgctagga atcatgctag tagcattcat cgttgctaaa
     3421 aagaataaat accctgttgt cgaaaaagga cagatggctc atgctggaaa aataataaaa
     3481 gaggcgattc ctagccttct attgattgtc atcattatcg gtggtatctt gacggggata
     3541 tttacagcag tagaagcttc agcgatagct gtcatttact ccctgttgat tgctatgttt
     3601 tactataaaa cggtaaaact aaaagatata cctaatatgt tgtttcaatc agtagcaatg
     3661 tcagggacga tcatgttctt attggctact tcttccatga tgtctttcgc catggcgttt
     3721 accggtattc ctcaggcaat tagttcgttg attatgggcg taacggataa taaatttatt
     3781 attctgttat tagtcaatct tgtgttatta ctggtgggta tgtttatgga tgtggcgcct
     3841 gctatcttga tttttacacc aatattttta ccaatcgtca caagtgtagg ggttgatcct
     3901 gtacattacg ggttgttttc aatcatgaat ctgtgtgtag gctccatcac gccgccagta
     3961 ggtacaggac tatatgtcgg tgcaagtgta ggaggcgtaa aagcagaggc aatgctgaaa
     4021 ccgcttcttc ctttttatgg ggttatctta gtcgtcttgt ttttgattac ttacttcccg
     4081 caaatcgtca tgtggctacc caatatgctg agttaggaga atagttatgt tgaacattac
     4141 aattagagga catgatcttt ccgaagtcca aacgatagaa gatcttgcag aaaaaacaaa
     4201 agaacaaggg attcatacac tgcagctcgc cttaggtctt tcgtttcctg aaataccttc
     4261 tggcgcaaat gagatcaatt ctgggatggg aaattatgtc aaacgagttt tagaaaagca
     4321 agaagtgacc gtaggaattt taagttgcta tatcaatatg attcatccag atttaacgat
     4381 acgacaagag ttgctgacaa agttcgagca gtatcttcgc tatgcttctt cctttggtgc
     4441 gtcgatggtt gcttcggaaa ctggatgtgt tttgccggaa atccaatata cggaagaaaa
     4501 tttcacagat gaagcatttg ctgaagctgt gtccgttatc cgtcgcttgg taaaagctgg
     4561 agaaaaatac caaatgatgg tggggataga accaggtttg aatcatccgg tgtattcttt
     4621 agcaagggta gagcagctga tccaagcagt agattcggac tatctaggaa tcattttaga
     4681 tccgaccaat ttgatcacaa gtagtgatta tcaagaacaa gtccaattgg tagaagaagc
     4741 ttttgaacgt tttggtgaaa agatatgtgc ggttcatttg aaagattttc gtgtcgaaca
     4801 ggaaaaaatc gtgccggtaa atttgggaga cggtgtgatc gagtacacaa aaatcaaaga
     4861 aatcatcatg aagaatcgtc cgtacctgta tgtggttctt gaagaaacaa aagatgatgg
     4921 gattcgttat ggaagaagtc ttcttgaata atgtaaagaa gacgttggtt aactaatatt
     4981 tagaatggag ggggcggggc tagaagatgc cacgtctctt cttttttata agagtatata
     5041 aaagcagctc tttttgatta caaaaaacgt ggtataataa aattgtgcat aagtgaacaa
     5101 aaggaggtgc ggcacatgct ccaaaagaaa atcagcggtg aacaagtcaa tacgatggtt
     5161 tctgatcagt atgaaatcta tcatgtcaaa gatgtcgtca gccaaaataa aacgatctat
     5221 catcatcatg atttctatga gatccatgct acattaaagg gagaagcgac tttttatttg
     5281 aacggacgac aatttacgat caaagcagga agtgtgctgc tcatccattc caatgatttg
     5341 catcgtatcg tccgccaatc ttcagatgta ttcgagcgtg tctatatctt tgtcacccct
     5401 tcttttctag aaagtcgttc cacgccatgg tctaatctat cggcttgctt tcaaccaatg
     5461 ggagagaaga ggagtcgtgt attgcaaata gaacaggaag tattgaagca aaaattatca
     5521 tttatcgacc atcctcctga taaaaaaatt tacggtgaag acataaaata tgaacaagct
     5581 ctggtagaat acttgatttt tctcaatcaa gttgtacaaa aagaagagag tgcgcaagaa
     5641 cagaatgctg tagttcctaa tgagcgtgtg gaacaaatgt tacaatatat ctcaaaaaat
     5701 ttatctcatc cgctttcttt agaggaaatg gaaaagcagt tttttgtcag caaataccat
     5761 atcacacgag aatttaaaaa gtacactgga tttacgtttc atcagtatgt aatgaagaaa
     5821 aagctgatct atgccaagca cttgttgcga gaataccgca gctctagtgc tgtatatagt
     5881 aaaagtggtt tttcttctta tccgcatttt ctgaaagcat tcaagagaga gttcggcata
     5941 acgcctaaag aatttttgaa acgtgataca cagaaacagt tggttcatta tgaacatttt
     6001 gaagatgaac aatgagaatg atcactaaaa aataaccgca gtagctgctg ttgtttacat
     6061 agaagcagaa actgcggtta ttttattttt atctatatat ttcaaaatat tttgaaaaaa
     6121 tgacgtacat attatatgaa tcagctgaat ttacgaaagt ctctcggcga tagtcctgtt
     6181 tttgctttga acaggcggct aaagtaagca aaattctcaa tccctatttc ttcggaaatc
     6241 attcgaaccg taaattctgt ataccgaaga aggtctttag cgtaagaaag ttttttgctg
     6301 atcaaataat cgattggtgg agaaccgata aatttggaaa aatctttgct cagctgatat
     6361 ttattcaata agaattgttg ttctaattga tcaagtgtaa tcgtttcttt gaaatgttcg
     6421 tctaagtatt gcttcagttt gtaaacgtag gcgggaatat cctcttcttc gaaatctagc
     6481 tgatactttt gcaaaatcag ttcattcagc aactcgtgga tagctacaga ggactggaaa
     6541 tcggtacggg cattcgtgcc ttgttgcatg tggatcaatt ctgtgatcaa atggtggatc
     6601 ttgttttctg atggatcccc tgttgaatgg aaggtgttat ggccgtcttt tgtgaactcc
     6661 tgataaaatg attggacgtt ctgaccattc aaatggatcc aatccatttc ccatggttct
     6721 tcgctgatcg tttgataacg ctgatattct tggcagtcaa taaaaaataa gtctccagct
     6781 tttagctgtt gctgattatc attatatgta agcgaacctt tgccagacaa agtaaacttt
     6841 atcaagtaag aaggtaaatg tgctcgttca gtatagtaag ggattggtgc tttgaagtaa
     6901 ccgatttctt ggatataaaa aaataaattc ctgcttcttt gtgtaggcgt caagatcact
     6961 ctttttgagt cttccgacca tgagtggttg gctttttccc agtaatccat gtaaacactc
     7021 ccaacttgtc aagataatgc aattatatac caaaatagtg tgaagcaaaa tataagattc
     7081 tgtattatga tagcattgtt aataaatgat aacgctttat taataaagtg ctaatcaaca
     7141 ataaagtaaa agaggtggag aaatgaatcc aacatttgtt gccatcgata tcggtgcatc
     7201 aagcggacga ctaatgaaaa gtacgaggga tgcaaaaggt tctttgatct tggaagaagt
     7261 acaccggttc aaaaacggct ttcggaacaa agacggtcac gatcgctgga agatcgatta
     7321 tcttatcaaa gagattttga ttggatttga aaaattaaag cagacaggga ttgatcgcct
     7381 ttttgcgggg atcgatacat gggcagtcga ttactgtctg attgatgaac aaggggatcg
     7441 gataaatgat ccgattgctt atcgggacaa gcgtaccaat ggagcagtag aacagttttc
     7501 tgaaatctat tctctagata agttgtatga gcagacaggg attcagatcc agcctttcaa
     7561 taccttgttc caattgtttg ctgaagaaaa agaaaatttg gcaaaagcac ataaattgtt
     7621 actgatacct gattatttag ggtatgtctt tactggtaac atggtgacgg agaaaactaa
     7681 tgcatctact atgcagctgc tgaatgcagg gacaaaagaa tgggaaatag aacttcttga
     7741 aaaaatcggt gtctcgaagg aattatttcc accattgaca gagccgggaa cgatactagg
     7801 agatctgcaa aaagaacaat tttcaaaata tgatttgcca gatgtgacct ttatcacagt
     7861 agcaagtcat gatacggcgt cagctgtatt aggaacgcca ggtcaaggag aacgttgggg
     7921 ttatatcagc agtgggacat ggtcgctttt agggattgaa acaaagatcg ccaatgtttc
     7981 tcgcaatgct ttttctgaaa attatacgaa tgaatggggt gcccataaca cggtgcgttt
     8041 tttgaaaaac atcatgggga tgtggttgat ccaggaagtt gcccgaatgc aagagtacga
     8101 gtacagttat gcggaattgg cagagcttgc tgcaaaagaa aaaccgctgc agcaagtgat
     8161 ggaaatcaat gaccctcgat ttttgaatcc cccaaatatg atagaagaga ttcagcaata
     8221 ctgttttgaa acagagcagc cagtccctgt ctcatcggga gctatcgctc gttgtgtgta
     8281 tgataatctt gctctttgtt acgcacaaga gctgatcaaa gtagaacact taactggaac
     8341 gaaattggaa aagctgcaca tcgtaggggg cggatcgaat aatcgtctac tgaatcaatt
     8401 gacagcagat tatgccggaa tcattgtaga agcaggacct agtgaagcca cagcaatcgg
     8461 gaatgttgcc atgcaaatgc tcagtacagg tcagttttcg aatatagaac aagcaagaaa
     8521 tgtaatcaaa cagtcgtttc catgtgaaac tttttaccca gatgaaaata agcaaaacac
     8581 gatggaatgg tacgtgaata aaatcaaagg agtaatcgta tgaacacgat tgaaagaaat
     8641 tacgaacaag caaaagaaaa atatgcaact atcggtgtag atacagatgc tgtactagaa
     8701 aagatacagg acatcaaaat ttctatgcat tgctggcaag gagatgatgt caaaggtttc
     8761 ttgacaccgg atggcgaatt gaccggtgga atcatggcga cagggaattt tccaggagcc
     8821 gcacgaacac ctgaagagct tagacaagat ctggagaagg cttattcgtt gataccgggc
     8881 aaacacaaat tgaaccttca tgctatctat ttggatacag aagaatctgt cgatctaaat
     8941 gaaattgagc cgaaacattt tgaaaaatgg gtagagtggg caaaaaaaga agaaatcggt
     9001 cttgacttca atccgacttt cttttcgcat ccaatgatga aagacggctt tacattggct
     9061 catccagata aagaagttcg tgaatactgg atcgaacacg ggaaaagaag ccgcaaaatc
     9121 gcggagtact ttgggaaaga aactgggcaa acaagtatca ataacttttg ggtacctgat
     9181 gggatgaaag ataatccgat tgatcgtttt accccacggg aacgtttaat ggaatcactc
     9241 gatgaaattt ttacagaaga gttagatgaa cggtatgtac aagatgcagt agaaagcaaa
     9301 ctatttggtt taggcgctga agcttataca gtcggttccc atgaatttta tatgggttac
     9361 gggttgactc gtgacaaact tgtttgtcta gatgctggac attttcatcc aacagaagtg
     9421 atttccaata aattatcttc attagcttta ttcagcaaag gaatcatgtt gcatgtttcc
     9481 cgtccagtgc gttgggattc agaccatgtg gttttgatgg atgatgaatt gcaggaaatc
     9541 gctaaagaat tagtccgtaa cgaattgttg gaaaaaacaa atatcggtct ggatttcttt
     9601 gatgcaacga tcaaccgtat cgctgcttgg gtcatcggta cgagaaatac acaaaaagca
     9661 ctgctgaaag ctatgctgga accagttgag cgattgaaag aaatggagct ggcttttgac
     9721 tttacttcaa gaatggctta tacggaagaa ttgaaagatt tcccgtatgc cgatgtatgg
     9781 aattatttct gttacaaaaa tcaagttcca gttggtttgg attggctgga agaagtccag
     9841 gaatatcaaa aagacgtgtt ggaattaaga aaataggagt gtggctatat tgaaaaaaat
     9901 agatatttta caagcgcctt atgtgaaaga aatgatcgaa acgacgtcga atctttatcg
     9961 cttgggatgg gatgaaagaa atggcgggaa tatttcttat ttactaaaag aagaagagat
    10021 cacaccgttc ttggatcctg aaaaagtgat ccgccgcatc tcgatgattt ttgatgccag
    10081 ccgtttagca ggatgctatt ttattgtaac tggttcagga agatatttta aaaatgtagc
    10141 gagcgaacct gccgagaatc ttggaatcgt tcgagttgct gaagatggca aaacactgga
    10201 acttttgtgg gggctggaaa acgaatgcct tcctacaagc gagttgccaa gtcatttcat
    10261 gagtcatatc gcccgtttag aagtagaccc agataatcgg atcgttatgc attgccatgc
    10321 tagtcatcta ttagcgatga gttttacgca tgaattagac gaacgttcct ttagtcgtac
    10381 gctatggcaa atgtgtacgg aatgcttagt tgtttttcca gaaggaattt ccatcattcc
    10441 gtggatggtt ccgggaacaa atgagattgg ggaagcaaca gctgaaaaaa tgaaagaaac
    10501 tcgattggtt ttatggccac aacatggaat ctacgggtca ggaaaagaca tggatgaagt
    10561 ctttggtttg atcgagacag cagaaaaggc agcagaagtc tatacgtacg tcaaagcgca
    10621 aggaccaatc ttgcaaacaa tcacggacga aaacctgtgg catttagcag atgcgtttgg
    10681 tgtcactcca aaagcaggtt atttggagga agttcatacg aaagcaggtg tctgacatgg
    10741 tgaaaaaagc tttttgcatg aaagtttacc cagaaaaaca agaagaatac aaaaaaagac
    10801 atgatgaact ttggccagaa atgcgtacag cacttcgtga acacggggtc atcgactatt
    10861 cgatctatct tgatccgagt acaagtttgt tatttggtta cttagtgatc gaagatgaaa
    10921 agttatggga aaaaatggca aatacatcta tcaatcaaaa atggtggaag tacatggaag
    10981 atatcatgga gacaaatcca gatgcctctc cagtcagttg ggaattgatg ccagtctttg
    11041 aactgtagca acaagcaact gggcaaccgg ttgcttgggg ttctttcgtt tttattgtga
    11101 aaaataatac aaattaggag gaatagaaaa tgagtaatcg aatgatttta aatgaaacat
    11161 cgtattttgg aaaaggagcg attgagtcga tcgtccctga atttcagcag cgaggattcg
    11221 gcaaagcagc tgtcattact gacaaaggat tgatcgagca cggtatagca acaaaagtaa
    11281 cagaacttct agataaagca gcaatcgctt atgctttata cgacgggatc gtacctaatc
    11341 caacgatcca aaatgtaaaa gatggagtgg cttttgttaa agaggcagag gcagactgtt
    11401 tgatcgcaat cggtggaggt tcgccaatcg atacggcaaa agccattggg attattttga
    11461 cgaatccaga gttttcagac gtcattagtt tagaaggtgt tgctgatacg aaaaatccat
    11521 gtttacctat attagctata ccaacgactt ctggaacagc agcagaagta acgatcaact
    11581 acgtcatcac agatgaagta aatcaccgaa aatttgtttg tgttgatcct catgatatcc
    11641 caatcgtcgc gttcatcgac agcgatatga tgatgggaat gccgaaaaaa ttggctgcat
    11701 caacaggtat ggatgctatg acgcatgcta ttgaagggtt tatcacaaaa ggcgcttggg
    11761 aaatgactga tatgctgcat ttaaaagcaa tcgaaatcat cggccattct ttggaagctt
    11821 ctgtagatgg cgatcaaaat gggcgagaaa aaatggcatt aggacagtat attgcgggaa
    11881 tgggcttctc aaatgtcggt ttaggactgg tacacgggat ggctcacccg ttaagtgcat
    11941 ggtacaacat tcctcatggc gtagcgtgtg cagcactttt gccaactgtc atgaaatata
    12001 acaaagaata tactggagaa aagtaccgtg agatcgctct tgttctaggg attaaaggag
    12061 cagctgaaat gtctttagaa gatgtaagag aagcggcttg tggtgagatc gatcggttga
    12121 gcaaagcggt tggaatccca gaaaccattt ctgaattagg tgtaaaagaa gcagatattc
    12181 cagcaattgc agaagatgct ttgcgagatg tatgcacacc aggaaatcca agagaaacga
    12241 cagtagaaga aatcattgct ttatatcaaa gtttgatgta aaaacgaagc ggatcttcaa
    12301 cattaaattg cctgtaaaga gaaatggtag tttatatctg gatatctctt ttaaattgat
    12361 ggtatttccg ctataatttt tcttataaag aaataggaga atattatatg gaaaataaaa
    12421 agccttatgg aactgtattg atcaaagcct ctaaaatact agactattta gcggaaaacc
    12481 aagatgtctc tttgcagacg atcgcaaagg gtgtgggaat gacctcttct acagtcttga
    12541 aaatactaga tactttgttg atgatcggct acgtaaataa aaattcagag aaaaactatc
    12601 gtttaggtgc aaagcttatt cgctacgcga ataaaaacat tgaacaaatc gatttggtag
    12661 agttgacttt accgttttta gaacagctgc agcaaaagat cgatgagact atccacttag
    12721 gggtattgga caacaatgag atcttgtacg tgaacaaact agatccaaaa catcaaacga
    12781 tccgcatgtc ttcaaaaatc ggaatcaccc gaccattata caattctgcg atgggcaaag
    12841 ccgtattagc agaattcagt gaagagcagt acactgatta tcttgaaaag catccgttga
    12901 tcccttacac agaatatacg atcaccaatg cattacggtt gaaaaaggaa atccaaacgg
    12961 taaaagaaac ccacatcgca tttgacgatg aagaaatcga acgtgatatt ttttgtatag
    13021 gagcctcaat cgtgaaaaac gaacaaatca ttggtgcatt tagtgtaagt atgccgaaat
    13081 atcgtttaac agatgagaca aaagaacaaa taatcagtgc cctcaagcaa acaaaaagcg
    13141 aaatcgagca aagaattcaa aaataatgaa aaaagcgttt gcaatttttc ttttctgccg
    13201 tataatatgg gtatagattt ccaagttata aaataaaatt tctaaatagg aaataggtgg
    13261 agaagatgaa aaaaattgat attttatctc gattgaaaaa cgccggagtg attgcggtgg
    13321 tacgcggaaa aagcaaagaa gaagctttga atgcttgcca cgcaatcatc aaaggtggtc
    13381 taactggtat tgagttgact tttacagtcc ctcaagcaga tcaagtaatc aaagaacttt
    13441 tgtcattcta caaagaccaa ccagaaatag tgattggcgc aggcacagta ttagacgctg
    13501 tcacagctcg cttggctatc ttagctggcg cagagtatat tgttagccct tcctttgatc
    13561 aagaaacggc agagatgtgc aatctgtatc aaattcctta cttaccagga tgtatgacta
    13621 ttaccgagat caaaacagcg ctgaaaagcg gggtagatat cgtcaaacta tttcctggaa
    13681 gcgcttatgg accaagtatc atttctgcct tcaaggcgcc gatgcctcaa gtcaatatca
    13741 tgccaactgg cggtgtgagt ctggacaata tgaaagagtg gttcgatgct ggtgtcgtaa
    13801 cagtcggagt aggtgggaac cttttagcac ccgcagctac aggagatttt gacaaagtaa
    13861 cggaagtcgc gcaacagtat gcagcaaaaa tgaaagaaat caagaggtag ctgcttatgg
    13921 gaaaagtagt gacactcgga gaaatcatgt tgcgattgtc cacagagcag ggaacccgta
    13981 tcgattcaag tgaacgattc gctgcccatt atggcggagg ggaagcaaac gtagcaattt
    14041 ctttagctaa ttacggacat gaagcgattt ttgctacaaa agtacctgat aacgcattgg
    14101 gaaaagcagt caaaaagcac ttgcaatgtt atggagtaga tacgaaacag gtattatctg
    14161 gtggatcaag actaggtact tattatatgg aaactggtag tggcgaacgc gccgcctcag
    14221 tgatctatga tcgtgccgga tcaagttttg ctgaaatgga tcatttggaa tggtcgctag
    14281 aggagttatt ccaaggaata gatatatttc atgtttcagg aatcacccca gctttgtcta
    14341 aaaaatggca aaaaatgacg aaacaggtga tagaagctgc aaaaaaagct ggctgcctga
    14401 tcagcttcga tatcaactat cgtggaaaat tatggacaca gcaagaagcc agcgaagtaa
    14461 tccatcagct tttaccttta gtagatatct gctcagctgg aaaaatggat gctctttatt
    14521 tgttaggtat cgagaaagcg ccagaagcag aagaacaacc tctaatttat tattatcaaa
    14581 aaatgcagga aaagttcccg aatattcagg tgttttattc gaccaagcgt accgttcatt
    14641 cagcaagtga aaatgaattg aaggggacat tgtggatgaa ccatgaatat gtggaatccc
    14701 aagtccatag aatcacccca atcgtggatc gagtaggtgg cggagatgct tttgcaggtg
    14761 gcgtgctcca tgggatttta tccaatatgt ctccgcaaga aatcattgat tttgctacgg
    14821 cagcttctgc cttgaagcat acgatccatg gggactgcaa ccaatttagt caagaagaag
    14881 tcgcacaatt tttagcatgc ggctctggaa aaatcattcg atagaaggag tagaagaaaa
    14941 tgcaagaaat ggaaacacgt tatacacaca gtccagaaga tatccgtcat tattcaacgg
    15001 aacaattgag aaaagaattt ttagtagaaa aggtatttga accagggaaa atcagtttga
    15061 cttatacgca caacgatcga atgatctttg gtggagtgac acctactgaa aaagaattag
    15121 aaatcacatt agacaaagaa ctaggtgttg attacttctt agagcgtcgt gaactaggtg
    15181 tgatcaatat cggtggacca ggattcatcg agatcgatgg gcaaaaagaa gaaatgaaaa
    15241 aacaagacgg atactatatc ggaaaagaaa cacgacatgt tgtattttct tctcaagatg
    15301 ctgaaaaccc agctaaattc tatattagtt ctgttccagc tcatcataaa tatccaaatg
    15361 taaaaatcag tatcgaccaa gtaaaaccaa tggagactgg tgaaggcctg acactgaatg
    15421 aacgtaaaat ctaccaatac atccatccaa atgtatgcga aagctgtcaa ttacaaatgg
    15481 gttatacgat tttagaacca ggaagcgctt ggaatacgat gccttgtcat acacatgaac
    15541 gtcgtatgga agcttatgtc tactttgatt atgccaatga agatacacgt gtgttccaca
    15601 tgatgggcaa accagatgaa acaaaacatt tagttgtaga caatgaacaa gccattattt
    15661 cgccaagctg gtcgatccat tcaggtgtag gtacaagcaa ttactcattc atctgggcaa
    15721 tgtgcggaga aaatatcacc tatacagaca tggatatggt gcaaatggat caattaaaat
    15781 aagcataagg gagagaaaat gatggaattt agtatggata tgtttcgttt agatggaaaa
    15841 gtagcattag tcaccggtgc tgtctatggt attggatttg agattgcacg ctctttagct
    15901 acagcaggag caaaaatcgt atttaataac ttgaatcaag aatcagttga tgaaggtata
    15961 gcgcattata aagaagcagg catcgatgca aaaggttatg tctgcgatgt gaccgatgaa
    16021 gaagcggttc aagcgatggt tcaacagatc aaagaagatg taggttcagt tgatattcta
    16081 gtcaacaatg cagggatcat caaacggaca ccgatgatcg aaatggatgc ggcagacttc
    16141 cgccaagtaa tcgacgtgga tttaaatgcc ccattcatcg tttcaaaagc agttattccg
    16201 gatatgatcg aaaaaggcgg cggtaaaatc atcaatatct gctccatgat gagtgaatta
    16261 ggacgcgaaa cggtcagtgc ttatgctgcg gcaaaaggcg gattaaaaat gctgacgaaa
    16321 aacatcgctt ctgaatacgg tcaatacaat atccaatgta atggaattgg accaggttat
    16381 attgctacac cgcaaactgc tccattacgc gaaacacaag aaaacggtga acgtcatcca
    16441 ttcgatcaat ttatcgttgg acgtacgcca gcagcacgct ggggcgagcc aatcgatttg
    16501 gctggaccat ctgtcttctt ggcttctagc gcatctgatt tcgttaacgg ccatgtcttg
    16561 tatgtggacg gaggcatact tgcctatatc ggaaaacaac cttaaaagag ctttagtaca
    16621 ctgtttagtg gagaggttgt gaaatcagcg gattgaacga ataccgaagg acagctgatt
    16681 aaacatcatt tattcgaaaa aagaggctgg gacagaagc
//
LOCUS       DACEND010000053        16693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEND010000053 DACEND010000000
VERSION     DACEND010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.054"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..1208)
                     /locus_tag="IW746_001463"
     CDS             complement(6..1208)
                     /locus_tag="IW746_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078369.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1325..1840
                     /locus_tag="IW746_001464"
     CDS             1325..1840
                     /locus_tag="IW746_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1078370.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2039..2485)
                     /locus_tag="IW746_001465"
     CDS             complement(2039..2485)
                     /locus_tag="IW746_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1078371.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IW746_001466"
     CDS             complement(2532..4010)
                     /gene="msr"
                     /locus_tag="IW746_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1078372.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4364..4993)
                     /locus_tag="IW746_001467"
     CDS             complement(4364..4993)
                     /locus_tag="IW746_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1078373.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4977..5441)
                     /locus_tag="IW746_001468"
     CDS             complement(4977..5441)
                     /locus_tag="IW746_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1078374.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5928..7196)
                     /locus_tag="IW746_001469"
     CDS             complement(5928..7196)
                     /locus_tag="IW746_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1078375.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGFTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7427..8164)
                     /locus_tag="IW746_001470"
     CDS             complement(7427..8164)
                     /locus_tag="IW746_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1078376.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8231..8800)
                     /locus_tag="IW746_001471"
     CDS             complement(8231..8800)
                     /locus_tag="IW746_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1078377.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8928..9800
                     /locus_tag="IW746_001472"
     CDS             8928..9800
                     /locus_tag="IW746_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1078378.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10142..11014
                     /locus_tag="IW746_001473"
     CDS             10142..11014
                     /locus_tag="IW746_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078379.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11011..12969
                     /locus_tag="IW746_001474"
     CDS             11011..12969
                     /locus_tag="IW746_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1078380.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13220..14071)
                     /locus_tag="IW746_001475"
     CDS             complement(13220..14071)
                     /locus_tag="IW746_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1078381.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14194..14661
                     /locus_tag="IW746_001476"
     CDS             14194..14661
                     /locus_tag="IW746_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1078382.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14924..15406)
                     /locus_tag="IW746_001477"
     CDS             complement(14924..15406)
                     /locus_tag="IW746_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078383.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15506..16369)
                     /locus_tag="IW746_001478"
     CDS             complement(15506..16369)
                     /locus_tag="IW746_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078384.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         4984 a         3461 c         2974 g         5274 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
      961 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
     1021 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
     1081 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
     1141 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
     1201 atttccatcc ctttctctct attagcatca tacatgattt attttacatg taaatatgca
     1261 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
     1321 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
     1381 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
     1441 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
     1501 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
     1561 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
     1621 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
     1681 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
     1741 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
     1801 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
     1861 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
     1921 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
     1981 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
     2041 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
     2101 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
     2161 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
     2221 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
     2281 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
     2341 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
     2401 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
     2461 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
     2521 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
     2581 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
     2641 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
     2701 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
     2761 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
     2821 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
     2881 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
     2941 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
     3001 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
     3061 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
     3121 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
     3181 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
     3241 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cggtctgatt
     3301 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
     3361 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
     3421 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
     3481 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
     3541 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
     3601 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
     3661 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
     3721 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
     3781 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
     3841 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
     3901 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
     3961 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
     4021 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
     4081 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
     4141 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
     4201 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
     4261 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
     4321 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacgtagtga
     4381 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
     4441 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
     4501 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
     4561 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
     4621 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
     4681 ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc atatcgccaa
     4741 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
     4801 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt tgcatattga
     4861 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
     4921 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
     4981 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
     5041 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
     5101 agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt ctgggctttt
     5161 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
     5221 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
     5281 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
     5341 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
     5401 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
     5461 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
     5521 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
     5581 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
     5641 tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc aaaaatttcc
     5701 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
     5761 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
     5821 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
     5881 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
     5941 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
     6001 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
     6061 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
     6121 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
     6181 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
     6241 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
     6301 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
     6361 gtttcgatta cattgttcaa tgtaaaacca taattaggcg cagtggcata acgacctgtt
     6421 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
     6481 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
     6541 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
     6601 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
     6661 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
     6721 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
     6781 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
     6841 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
     6901 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
     6961 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
     7021 tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc tgtttctgaa
     7081 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
     7141 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
     7201 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
     7261 agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct ttttctacat
     7321 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
     7381 ataactattt aacaagtaat tctctttgga tattatccgt tatggattat acttcctgtg
     7441 tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct atcgcaaatg
     7501 ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct tcatgaagtt
     7561 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
     7621 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
     7681 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
     7741 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
     7801 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
     7861 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
     7921 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
     7981 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
     8041 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
     8101 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
     8161 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
     8221 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
     8281 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
     8341 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
     8401 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
     8461 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
     8521 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
     8581 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
     8641 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
     8701 gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
     8761 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
     8821 ttactttata acggacggct tatcatgtct aatacttata aaacatactt aactattcct
     8881 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
     8941 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
     9001 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
     9061 tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt cgatatcttt
     9121 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
     9181 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
     9241 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
     9301 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
     9361 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
     9421 ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc catccgcagg
     9481 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
     9541 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
     9601 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
     9661 atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa gcttcactga
     9721 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
     9781 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
     9841 ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt atttcttcct
     9901 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
     9961 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    10021 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    10081 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    10141 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    10201 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    10261 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    10321 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    10381 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    10441 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    10501 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    10561 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    10621 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    10681 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    10741 gaattattca agactggtga atcatttacg actacttcta caacgaaccc atgcgcaaca
    10801 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    10861 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    10921 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    10981 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    11041 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    11101 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    11161 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    11221 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    11281 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    11341 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    11401 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    11461 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    11521 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    11581 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    11641 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    11701 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    11761 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    11821 aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg tgtgacagaa
    11881 ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    11941 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    12001 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    12061 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    12121 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    12181 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    12241 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    12301 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    12361 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    12421 gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa caccgtggtt
    12481 aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat cgcaaatggg
    12541 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    12601 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    12661 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    12721 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtagaa
    12781 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    12841 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    12901 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    12961 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    13021 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    13081 gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac tcttcttatt
    13141 acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    13201 ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    13261 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    13321 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    13381 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tctttacctg
    13441 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    13501 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    13561 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    13621 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    13681 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
    13741 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    13801 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    13861 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    13921 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    13981 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    14041 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    14101 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    14161 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    14221 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    14281 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    14341 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    14401 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    14461 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    14521 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    14581 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    14641 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    14701 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    14761 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
    14821 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
    14881 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
    14941 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
    15001 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
    15061 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca acaagatacg
    15121 ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc gtttgaacac
    15181 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
    15241 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
    15301 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
    15361 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
    15421 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
    15481 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
    15541 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
    15601 acccataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
    15661 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
    15721 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
    15781 tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt acttgatttg
    15841 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
    15901 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
    15961 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
    16021 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag
    16081 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
    16141 atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta gccaaagcca
    16201 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
    16261 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
    16321 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
    16381 tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
    16441 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
    16501 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
    16561 ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
    16621 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
    16681 tggatttttc ttt
//
LOCUS       DACEND010000054        16585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEND010000054 DACEND010000000
VERSION     DACEND010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.055"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..546
                     /locus_tag="IW746_001479"
     CDS             <1..546
                     /locus_tag="IW746_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1078385.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IW746_001480"
     CDS             735..1403
                     /locus_tag="IW746_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1078386.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="IW746_001481"
     CDS             complement(1603..1989)
                     /locus_tag="IW746_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078387.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="IW746_001482"
     CDS             complement(1961..3343)
                     /locus_tag="IW746_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1078388.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="IW746_001483"
     CDS             complement(3499..4686)
                     /locus_tag="IW746_001483"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1078389.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="IW746_001484"
     CDS             complement(4693..5724)
                     /locus_tag="IW746_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1078390.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="IW746_001485"
     CDS             complement(5740..7194)
                     /locus_tag="IW746_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078391.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            7369..8274
                     /locus_tag="IW746_001486"
     CDS             7369..8274
                     /locus_tag="IW746_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078392.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            8365..9042
                     /locus_tag="IW746_001487"
     CDS             8365..9042
                     /locus_tag="IW746_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1078393.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9323..10219
                     /locus_tag="IW746_001488"
     CDS             9323..10219
                     /locus_tag="IW746_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1078394.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="IW746_001489"
     CDS             complement(10274..11644)
                     /locus_tag="IW746_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078395.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIR
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="IW746_001490"
     CDS             11882..12628
                     /locus_tag="IW746_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078396.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="IW746_001491"
     CDS             12625..13464
                     /locus_tag="IW746_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1078397.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="IW746_001492"
     CDS             13465..14415
                     /locus_tag="IW746_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1078398.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="IW746_001493"
     CDS             14556..15308
                     /locus_tag="IW746_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078399.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="IW746_001494"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="IW746_001494"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1078400.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5396 a         3091 c         3326 g         4772 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaata actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatatctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagtgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gatctactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca atttctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctgcattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacaaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accacagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tctgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagaatg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa atatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 agtttttgtc cagtaattgt tgtaacaatt actggttttt ttgtattttt tacatttttt
    16561 taagagaatt tgataaaaat caaaa
//
LOCUS       DACEND010000055        16507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEND010000055 DACEND010000000
VERSION     DACEND010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.056"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..1182
                     /locus_tag="IW746_001495"
     CDS             184..1182
                     /locus_tag="IW746_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078401.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="IW746_001496"
     CDS             1294..1803
                     /locus_tag="IW746_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1078402.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="IW746_001497"
     CDS             complement(1833..2492)
                     /locus_tag="IW746_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1078403.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="IW746_001498"
     CDS             complement(2494..3258)
                     /locus_tag="IW746_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1078404.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="IW746_001499"
     CDS             complement(3266..3943)
                     /locus_tag="IW746_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1078405.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="IW746_001500"
     CDS             complement(3980..5365)
                     /locus_tag="IW746_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1078406.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5334..5588)
                     /locus_tag="IW746_001501"
     CDS             complement(5334..5588)
                     /locus_tag="IW746_001501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078407.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="IW746_001502"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW746_001502"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IW746_001503"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IW746_001503"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1078408.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="IW746_001504"
     CDS             6304..7161
                     /locus_tag="IW746_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1078409.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="IW746_001505"
     CDS             complement(7242..7577)
                     /locus_tag="IW746_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078410.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="IW746_001506"
     CDS             complement(7644..9014)
                     /locus_tag="IW746_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1078411.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="IW746_001507"
     CDS             complement(9092..9628)
                     /locus_tag="IW746_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078412.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="IW746_001508"
     CDS             9937..11418
                     /locus_tag="IW746_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1078413.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11521..12228)
                     /locus_tag="IW746_001509"
     CDS             complement(11521..12228)
                     /locus_tag="IW746_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1078414.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12307..12645)
                     /locus_tag="IW746_001510"
     CDS             complement(12307..12645)
                     /locus_tag="IW746_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1078415.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12756..13718)
                     /locus_tag="IW746_001511"
     CDS             complement(12756..13718)
                     /locus_tag="IW746_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1078416.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13733..15064)
                     /locus_tag="IW746_001512"
     CDS             complement(13733..15064)
                     /locus_tag="IW746_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1078417.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15072..15752)
                     /locus_tag="IW746_001513"
     CDS             complement(15072..15752)
                     /locus_tag="IW746_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1078418.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15971..16450)
                     /locus_tag="IW746_001514"
     CDS             complement(15971..16450)
                     /locus_tag="IW746_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1078419.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4883 a         3441 c         2737 g         5446 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    11461 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    11521 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    11581 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    11641 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    11701 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    11761 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    11821 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    11881 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    11941 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    12001 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    12061 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    12121 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    12181 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    12241 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    12301 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    12361 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    12421 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    12481 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    12541 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    12601 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    12661 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    12721 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    12781 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    12841 ttcgcaccac ttgctaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    12901 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    12961 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    13021 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    13081 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    13141 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    13201 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    13261 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    13321 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    13381 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    13441 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    13501 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    13561 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    13621 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    13681 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    13741 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    13801 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    13861 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    13921 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    13981 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    14041 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    14101 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    14161 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    14221 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    14281 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    14341 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    14401 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    14461 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    14521 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    14581 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    14641 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    14701 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    14761 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    14821 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    14881 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    14941 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    15001 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    15061 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    15121 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    15181 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    15241 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    15301 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    15361 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    15421 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    15481 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    15541 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    15601 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    15661 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    15721 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    15781 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    15841 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    15901 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    15961 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    16021 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    16081 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    16141 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    16201 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    16261 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    16321 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    16381 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    16441 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    16501 ttcaaaa
//
LOCUS       DACEND010000056        16460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEND010000056 DACEND010000000
VERSION     DACEND010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.057"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            346..3180
                     /locus_tag="IW746_001515"
     CDS             346..3180
                     /locus_tag="IW746_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078420.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3371..3862
                     /locus_tag="IW746_001516"
     CDS             3371..3862
                     /locus_tag="IW746_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1078421.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3965..4954
                     /gene="manX"
                     /locus_tag="IW746_001517"
     CDS             3965..4954
                     /gene="manX"
                     /locus_tag="IW746_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1078422.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4987..5790
                     /locus_tag="IW746_001518"
     CDS             4987..5790
                     /locus_tag="IW746_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1078423.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5811..6725
                     /locus_tag="IW746_001519"
     CDS             5811..6725
                     /locus_tag="IW746_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1078424.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6808..7182
                     /locus_tag="IW746_001520"
     CDS             6808..7182
                     /locus_tag="IW746_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1078425.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7321..7566
                     /locus_tag="IW746_001521"
     CDS             7321..7566
                     /locus_tag="IW746_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1078426.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7738..8802
                     /locus_tag="IW746_001522"
     CDS             7738..8802
                     /locus_tag="IW746_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1078427.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9048..9473
                     /locus_tag="IW746_001523"
     CDS             9048..9473
                     /locus_tag="IW746_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1078428.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9466..9915
                     /gene="nusB"
                     /locus_tag="IW746_001524"
     CDS             9466..9915
                     /gene="nusB"
                     /locus_tag="IW746_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1078429.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10012..10866
                     /locus_tag="IW746_001525"
     CDS             10012..10866
                     /locus_tag="IW746_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1078430.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10867..12204
                     /locus_tag="IW746_001526"
     CDS             10867..12204
                     /locus_tag="IW746_001526"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1078431.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12209..12433
                     /locus_tag="IW746_001527"
     CDS             12209..12433
                     /locus_tag="IW746_001527"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1078432.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12435..13325
                     /locus_tag="IW746_001528"
     CDS             12435..13325
                     /locus_tag="IW746_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1078433.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13334..14152
                     /locus_tag="IW746_001529"
     CDS             13334..14152
                     /locus_tag="IW746_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1078434.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14259..14708
                     /locus_tag="IW746_001530"
     CDS             14259..14708
                     /locus_tag="IW746_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1078435.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14726..16408
                     /gene="recN"
                     /locus_tag="IW746_001531"
     CDS             14726..16408
                     /gene="recN"
                     /locus_tag="IW746_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1078436.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         5440 a         2731 c         3704 g         4585 t
ORIGIN      
        1 aaataaaagt tgtagtggag gctacgaagt ttactttgtc tttttacact taatattttt
       61 ttgccaattt ggaatcagga tgttttgata actatcgttt tcaattgatg gaagctatat
      121 tgtttgctat cagtgaaata attatatggt aaacagtgtt ctttttcttg tttccattat
      181 aaaaatcgta tcgggatttt tcgttctttt aaattgaaca tttctatcta tccgcgtgtt
      241 atcaacaaaa caaaatcagg taaatagttg acgaaacaga aataaataca tagtattatg
      301 ttttgaagcc taagtacaat agtctataga aaaggagcga taccattggc aaaacgcatt
      361 gaccgaattt atatgtatgt caaagaaaaa accgctcatt taacaaaggc agaatatgat
      421 caaggagtaa caacacagga aattgctgaa gtgttaggga ttcagcgaac aaatagcagt
      481 aaagatctga atcagctagt gcgcgaagga aaactgataa agacagacgg acgaccagta
      541 cgttacatat atcaaacact cgttacgcac gtaaagccag ttacaaaaca tgtagtcagt
      601 tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc aaatagatac taaggatatc
      661 tttgcaaaaa tcattggagc taacggcagc atgaaaaatt cagtggaaca ggcaaaagct
      721 gcgattttgt atccgccaag aggattaaat tgtttgatta caggacctac cggatcagga
      781 aagacttatt ttgcacacgc gatgtatcat tttgcaaaag cgcatcatgt aatagatgag
      841 gaaacggaac tgattgtttt caattgtgct gattatgcca ataaccctga gcttttgatg
      901 agtcatttat ttgggtacgc aaaaggtgct tttacaggcg cagatgaaga aaaaatgggg
      961 attatcgatc aagcggatgg cggtatgctg tttttggatg agattcaccg gttgccgcct
     1021 gaaggtcaag aaatgatctt ttactttatg gatcatggaa cttacagtcg tcttggggaa
     1081 acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg cgaccacaga agacccaggt
     1141 tcttctctat tagaaacttt tgttcggcgt atcccaatca atatcaaact tccggctttt
     1201 gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa tcatgattgc ccacgaagca
     1261 aaccgcacgc agcgaaagat ttctttgaca gaagatgtag caaaagcatt gatcggcagt
     1321 gtgacctatg gaaatatagg acaattgaag tcaaatatcc agttagtatg tgcacgagga
     1381 tttctaaatc atatgcaatc acccgaaatc tcgatcacaa tcgatgattt gacagaaggg
     1441 attcgtagcg gattgattca attagcaagc aaccgcagtg caatggcaga gctttctaaa
     1501 atattggagc caaagataac tgtagcacca aatgatacga tcatgaaaat ccaatcggac
     1561 tcctatgagt taccttataa tctatatgat atcatcggag ataaagcagc tttactgaag
     1621 tcggatggtt tggatcaaga agcaatcaat catttcattt ctacagatat caacatccat
     1681 ctgaagtctt tctataaaga ccatggtttt tcttttaatg cagacagcaa attagcagaa
     1741 tttgtagatc cgaaaatcat agaagtgacc aatcagattt atgtaatggt caaaagatct
     1801 ttaccatatg agtttcagca aaactttatt tatgctatga gcttgcatat cagttctttt
     1861 ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg ataacatccg tgagatggct
     1921 tttgattttc cagatgaata tgctgttgcg aaagaagtgc gtagttatat cgaaacgtat
     1981 tttcaagtgg agattccaga aagtgaagac tattacttga ccgttctact cgtctcgtta
     2041 cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg cagctcacgg aaacagcaca
     2101 gcaagcagta tggtccaagt tgtccaacag ttgttagatg cagagaacgt acgagcagta
     2161 gacatgcctt tggatatgga tccgaaaaca gcattggacc gaattgaacg caatgtacaa
     2221 gaagtagatg aaggaagcgg ggtcatactc ttagtagata tggggtccct tgcgtcgttt
     2281 aattcgcaaa ttcaaagaga cactggaatt ccagtacgta ctgttgatat ggtaacaact
     2341 tctctagttt tggaaactgt acgaaaggtc tcagtattag ggacagatct agatatgttg
     2401 catgaatcat tgaaaaattt tcgaggttac gcagaaatat cttctgtaga atctgttcag
     2461 gaagcaaaac ctattcataa gaaaaaagct gttttggcta tctgcgcatc tggcgaaggt
     2521 acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa acaagcgtca agaaacagaa
     2581 ttagaagttc tagctttgtc gattgtggaa gtgaaagaag aactgccagt tatccaaaaa
     2641 gaatatcaga tcatagctac tactgggatt actgatttga atatccaagc cccatttatt
     2701 cctttagaac gttttattga tcaaaatatc gaagtcattt tggatcagtt gctgatggaa
     2761 tcagaattgg aggaaactga attcatctcc ctagatgaag agtcagctaa aaatacgtgt
     2821 gttgagttta tctctgataa cttcatcttt attaatggta gtaagttaat cgatccaatg
     2881 tggcaatttt ctacacaaat ctctcagacg acgggaatcg gcgatgaaga atatggattc
     2941 aaaatcaacc tagtgatgca cacagcagga atgatcgaac gtattattcg gaatgaacca
     3001 ttgactgtgg aagaaaacga attgacaaat acaacgaatg atcctcttta tagccaattg
     3061 gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa aagtgccgat agaagaaatg
     3121 tattatctgc tacgtctagt ccataatcag ttagataaaa aagaatacac tgtgccatga
     3181 atacacaaaa acgatacact tttatagtgt atcgtttttg tgtattggtt aagaatcctt
     3241 taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc
     3301 taggaggtgg ttgttcacac agaaatgata ggggtttctg tgaagtagtg aggaagaagg
     3361 agttttgcag atggatattc gattggcacg tatagatgat cgtttaatcc acgggcaagt
     3421 cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt
     3481 cgctcaagat aaattgagaa agtttctact aaaagaagct gcaccgccgg ggatcacagc
     3541 aaatgtaata accgtgcaga aaatgattca aatctatcat agtggattat tatcacgagc
     3601 aaaagtgatg cttttattca ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt
     3661 acagttgaag tcgttgaata tcggcggtat gagttttact gaaggcaaat caatgattac
     3721 caattttgtt tcagtaaatc aacaagatat tgattccttt cgttacttga acagtcaaga
     3781 agtggagtta gagattcgaa aagtacctgc tgacaggaaa atccttttga tggaactact
     3841 agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa aaggtcaaat aattctgttg
     3901 taatggttag caattattaa tatttttgaa tttgttacaa caaaagtaaa taggaggtat
     3961 aaatatggta gggattatcc tagcaagtca tggacaattt gctgaaggaa tcttacaatc
     4021 cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga
     4081 aggtccagat gacttgagag ccaaattgga agaagcggtt gcttcttttg ataatcaaga
     4141 cgaagtgtta ttcttagttg atttatgggg cggaacaccg ttcaatcaat caaacacatt
     4201 attcgaagag cacaaagata aatgggcaat cgtcagcggt ttgaatttac caatgttgat
     4261 tgaagcctac gcatcacgct tttcaatgga atctgctcat gaaattgcag cacacatcat
     4321 tgaaacagca aaagacggtg taaaagtaaa accggaagaa ttagaaccgg cagaagcacc
     4381 gaaagcagca gttgaagacg cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg
     4441 agacggaaaa atcaaatatg tattagcacg tgttgactca cgtctgttac atggacaagt
     4501 tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt
     4561 atcaaaggat gatctgcgta agagactaat cgaacaagct gctcctccag gtgtaaaagc
     4621 aaatgttatt cctattagca aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac
     4681 caaagcattg cttttatttg aaaatcctga agacgtattg acagctgttg aaggtggcgt
     4741 agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag
     4801 taaagtattg tctatgggac aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg
     4861 tgtgaaattc gatgtacgta aagtacctaa tgattcacgc gataacatgg atgatatttt
     4921 gaaaaaagca aaagctgaat tagcaaaagc gtaataaata atattaaaat ttataggagg
     4981 cttatcatgt ctattatttc aataatttta gtcgtattag ttgcctttct agctggtatg
     5041 gaaggtattc ttgatgaatt tcaatttcat caaccactag tggcatgtac attgatcggt
     5101 ttagttaccg gtaacctcga agctggtatc gtacttggcg gtacgcttca gatgatcgct
     5161 ttaggttggg caaatatcgg tgccgctgta gcaccagatg ctgcattagc atcagtagca
     5221 tcagcaatta ttttagtttt aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg
     5281 attgccgttc ctttggctgt tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct
     5341 gttccaatcg ttcatttgat ggatgcggct gctgaagaag gaaacatccg taaggttgaa
     5401 atgtggcaca tcattgctgt atgtttacaa ggggttcgta tcgcggtccc agcagcagcg
     5461 ttgctattta tcccagcaca aactgtgcaa tcattccttg aatcaatgcc agcatggtta
     5521 acagatggta tggctatcgg tgggggaatg gtcgtagctg taggttacgc tttggtaatc
     5581 aacatgatgg ctacaaaaga agtatggcca ttcttcgtta ttggtttcgt tgtagctgca
     5641 atttctcaat taacattgat tgctctaggt gctttaggtg ttgcattagc tcttatttac
     5701 ttgaaccttt ctaaaatggg tggttcatca aataatggag gcggaagcaa ctctggtgac
     5761 cctttaggcg acattttgaa tgactattaa tcttgaagga ggaaaataaa atggcagaag
     5821 aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc gtggcgctct accttcattc
     5881 aaggttcatg gaactacgaa cgtatgcaaa acggtggctg ggcattttct atgattccag
     5941 caatcaaaaa attatacaaa acaaaagaag aacgttctgc agcattaaaa cgtcacttgg
     6001 aattctttaa tacacaccca tacattgctt ctccaatttt aggggttaca ttggctctgg
     6061 aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc gattcaaggg gttaaagtag
     6121 gtatgatggg tcctttagct ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa
     6181 tgctaggtgc actaggtgct tcattagcta tgggaggaaa tatccttgga ccaatcatct
     6241 tcttcttagg ttggaacatt attcgttggg cattcatgtg gtatacacaa gaatttggct
     6301 acaaagctgg ttctaaaatt actgatgacc tttctggcgg attgttacaa gacgtaacaa
     6361 aaggtgcttc aattttaggg atgttcgtat tagctgccct tgtacaaaga tgggtatcaa
     6421 tcaaattctt accagttgta tcaactgtaa aattagataa aggtgcttac atcgaatggg
     6481 ataaattgcc ttcaggtggt gaaggaatca agagtgcatt tgaacaagtt aataatggct
     6541 tagccctttc tcctgaaaag gtaacaacat tacaagacaa cttagaccaa ttgattcctg
     6601 gtttagctgc attaggatta acattgttat gtatgtggtt gcttaagaaa aaggtttctc
     6661 caatcgttat catccttggt ttattcgtag tcggtgttgt tggacacgtc atcggacttt
     6721 tataatccat aaaaatatag ctcgggcgtt gtcccgagct ttttttatat agtatactgt
     6781 tcataaacaa atatctggag ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg
     6841 gtaattgatg ctacttcgtt tacgggtctg acagattacg ggcaaatcat gataggtgat
     6901 aatggttttg agttttacca tgcgcgagat agacgcaaat atatccaaat tccatgggaa
     6961 gaagtggatt atgtactagc atcagtcatg ctaaagggaa aatggattcc tcgatatgcc
     7021 attcaaacga aaaaaaatgg tacatacaca ttttcttcca aagaacctaa aaaagtgctt
     7081 cgtgcgatca gagaatatgt agatgaagat agaatggtac aatcattgag tttctttgat
     7141 gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct
     7201 aagaggaggt tttctttatt tttttacttt gcagaagaaa atatgaaata gcaaaaccaa
     7261 ttatttgtcc cctttaaatc ccatatgcta aaattgatta gcaaagaagg aggttatatt
     7321 atgagttggt tatggacatt gattgttgga gccgtgatcg gagcaattgc aggtgcgatc
     7381 accagtaaag gaaattcgat gggctgtgta acgaatatca ttgctggact agtcggttca
     7441 agtattggac aagcgatttt cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt
     7501 ccatctattt taggcgcagt gattttggta gcagttgtct catttttctt tggaaaacga
     7561 tcataagttt ttacaaaata gtaaactaaa aggagttgca gcgagactgt gactcctttt
     7621 tggctttatt tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt
     7681 acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg
     7741 atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg
     7801 gttaccagtc cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct
     7861 gttattactt tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca
     7921 gcgcaggctc aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca
     7981 aacttagtac gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt
     8041 ttagaatatt cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg
     8101 atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt
     8161 catattgcgg atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag
     8221 attgaagtcg caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct
     8281 tttgatacga tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa
     8341 aaaatcatcg aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac
     8401 gtttccgata tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt
     8461 tatcaaatag tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact
     8521 ggtgtccaat tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca
     8581 tttggacatt ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct
     8641 tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac
     8701 ttaccaggaa tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat
     8761 cgtgtgttga ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag
     8821 taagcgtaaa aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac
     8881 actatagaag aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat
     8941 agcagtttgc agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata
     9001 gagaattcgg atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa
     9061 aaaatttgat tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca
     9121 tcgaagtaat tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa
     9181 cttttgccag caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact
     9241 tagcaaacga ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt
     9301 cagttcctaa agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga
     9361 cggatatcga tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag
     9421 aacaaccaac aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta
     9481 tcaagacacg agattcggga aatggcattg caggctttat ttccattaga cttcaatgca
     9541 gacctaacaa aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc
     9601 aacgaagatg aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt
     9661 gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc
     9721 aatcggatct caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat
     9781 gtgacagatg taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt
     9841 agtgatgacc gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta
     9901 gaagctgggg cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg
     9961 ggaaaattct atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa
    10021 ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa
    10081 aaattgaaag ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca
    10141 gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca
    10201 ttggtagagc gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa
    10261 tacaatcaag atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata
    10321 gatgaagaaa agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg
    10381 atgaatttag gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc
    10441 atcatgaaaa tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc
    10501 ggacgaagca atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact
    10561 gttactgttg ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt
    10621 ttagtcgtag caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct
    10681 gtagtgatag atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa
    10741 tttgatgaag tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca
    10801 atgactatca cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt
    10861 gaatagatgg cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa
    10921 tttgatgccg atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt
    10981 ttacgtgcca atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct
    11041 attatgttca aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc
    11101 cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag
    11161 cacatggagc ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag
    11221 ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga
    11281 atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa
    11341 agaagatatc caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca
    11401 gcggacaatg ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg
    11461 atcatcggtc gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt
    11521 gctcgggcga tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat
    11581 accacgatcg ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa
    11641 ttagctgttc ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg
    11701 cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt
    11761 tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag
    11821 ttgaaacagc gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg
    11881 atcgcattaa gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc
    11941 caagaaactg cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa
    12001 cagcagcagc gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc
    12061 atgggaagag gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag
    12121 attcatcaag gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta
    12181 caagagatag acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca
    12241 agaattagaa aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct
    12301 agattcgttt aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc
    12361 agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa
    12421 cgaggaaagc tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa
    12481 aagaaatctt gtctttttta gatgagcata caacggatag aggactctat gatgcgatga
    12541 gttattcagt gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg
    12601 cttcctttga tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg
    12661 tgcatacgta ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta
    12721 gaggaaaacc taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg
    12781 gtttgcttac aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc
    12841 ttctgctgct tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag
    12901 ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg
    12961 aaagaaagac agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc
    13021 aacctgaaga aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc
    13081 agataagaga tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg
    13141 gtaaagatga acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac
    13201 gtgaggcgtt ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg
    13261 acgttttttc ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga
    13321 aatgagggaa agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt
    13381 tgaaacaaga gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa
    13441 cgagcggttt gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg
    13501 taaaaaactt ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt
    13561 tgatctatct gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac
    13621 tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca
    13681 gctagcttgg aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg
    13741 ttatgcaaaa cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc
    13801 ttttatttca ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga
    13861 agtgattgct ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg
    13921 aattgttcgt gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc
    13981 acaaggtgga tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa
    14041 tatcgagttc ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat
    14101 taatatggca gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac
    14161 tgtgaaaatc aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat
    14221 cattgattaa gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc
    14281 gtttgattac tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa
    14341 tcctgaaaag caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac
    14401 tgaaactgat caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa
    14461 caaacgaaga tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg
    14521 accaaatgga aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa
    14581 atttgattga taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg
    14641 ttttgatgat tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt
    14701 acctttaagt tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat
    14761 tattccggaa ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg
    14821 tgcgggtaaa tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag
    14881 cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa
    14941 acaagaagga ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct
    15001 gattgttcga cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat
    15061 tatcacatta gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa
    15121 cgaacatcag gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga
    15181 tgtagctttc caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct
    15241 ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat
    15301 gctccatttc cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa
    15361 attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc
    15421 aggttacgga gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt
    15481 ttcagaactg caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat
    15541 ccaaagtgct tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt
    15601 attggagtta gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca
    15661 gttgaaacgt aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac
    15721 cgaagagcta gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact
    15781 agagcaaaaa gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa
    15841 aatcgcaaaa gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa
    15901 cactgagttt gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt
    15961 tgaccaaatc gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa
    16021 agtcgcttct ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc
    16081 tgagcaaggt gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt
    16141 tgcacaggcc atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat
    16201 cactcatctg cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt
    16261 gattgatgga cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga
    16321 aattgcacgt atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga
    16381 actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc
    16441 agctgatagc tatattttat
//
LOCUS       DACEND010000057        16276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEND010000057 DACEND010000000
VERSION     DACEND010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.058"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..436)
                     /locus_tag="IW746_001532"
     CDS             complement(<1..436)
                     /locus_tag="IW746_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1078437.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLE"
     gene            complement(566..1276)
                     /gene="budA"
                     /locus_tag="IW746_001533"
     CDS             complement(566..1276)
                     /gene="budA"
                     /locus_tag="IW746_001533"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1078438.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1289..2953)
                     /gene="alsS"
                     /locus_tag="IW746_001534"
     CDS             complement(1289..2953)
                     /gene="alsS"
                     /locus_tag="IW746_001534"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1078439.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3158..3820)
                     /locus_tag="IW746_001535"
     CDS             complement(3158..3820)
                     /locus_tag="IW746_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1078440.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3830..4645)
                     /locus_tag="IW746_001536"
     CDS             complement(3830..4645)
                     /locus_tag="IW746_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1078441.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4907..5350)
                     /locus_tag="IW746_001537"
     CDS             complement(4907..5350)
                     /locus_tag="IW746_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1078442.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5381..5494)
                     /locus_tag="IW746_001538"
     CDS             complement(5381..5494)
                     /locus_tag="IW746_001538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1078443.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5484..5822)
                     /locus_tag="IW746_001539"
     CDS             complement(5484..5822)
                     /locus_tag="IW746_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1078444.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5810..6187)
                     /locus_tag="IW746_001540"
     CDS             complement(5810..6187)
                     /locus_tag="IW746_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1078445.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6411..7604
                     /locus_tag="IW746_001541"
     CDS             6411..7604
                     /locus_tag="IW746_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078446.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7770..8192)
                     /locus_tag="IW746_001542"
     CDS             complement(7770..8192)
                     /locus_tag="IW746_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1078447.1"
                     /translation="MQTFLTKASQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8782..9237
                     /locus_tag="IW746_001543"
     CDS             8782..9237
                     /locus_tag="IW746_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078448.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9399..10571)
                     /locus_tag="IW746_001544"
     CDS             complement(9399..10571)
                     /locus_tag="IW746_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1078449.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10777..12206)
                     /locus_tag="IW746_001545"
                     /pseudo
     CDS             complement(10777..12206)
                     /locus_tag="IW746_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12203..13705)
                     /locus_tag="IW746_001546"
                     /pseudo
     CDS             complement(12203..13705)
                     /locus_tag="IW746_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13702..16029)
                     /locus_tag="IW746_001547"
     CDS             complement(13702..16029)
                     /locus_tag="IW746_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1078450.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4646 a         3241 c         2880 g         5509 t
ORIGIN      
        1 tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta
       61 aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg
      121 aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt
      181 tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc
      241 cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag
      301 tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat
      361 cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct
      421 gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc
      481 agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac
      541 cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag
      601 cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg
      661 acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc
      721 caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca actccttgga
      781 ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg
      841 gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac
      901 ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg
      961 cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa
     1021 aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta
     1081 attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt
     1141 caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa
     1201 tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata
     1261 agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc
     1321 ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg
     1381 caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag
     1441 cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc
     1501 gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca
     1561 aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa
     1621 atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa
     1681 tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag
     1741 gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa
     1801 cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga
     1861 tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg
     1921 ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat
     1981 caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt
     2041 cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg
     2101 cttcatattc gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca
     2161 tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac
     2221 gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta
     2281 attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg
     2341 gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac
     2401 ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat
     2461 cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat
     2521 tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg
     2581 gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt
     2641 ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg
     2701 tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa
     2761 tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt
     2821 caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga
     2881 atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca
     2941 ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat
     3001 aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta
     3061 tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa
     3121 actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca
     3181 atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc
     3241 attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac
     3301 tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg
     3361 acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag
     3421 caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac
     3481 aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta
     3541 gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg
     3601 ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa
     3661 aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca
     3721 agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga
     3781 attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca
     3841 ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa
     3901 caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc
     3961 caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga
     4021 atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg
     4081 ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga
     4141 tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca
     4201 caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca
     4261 cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc
     4321 cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag
     4381 cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat
     4441 tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc
     4501 aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag
     4561 cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca
     4621 tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat
     4681 ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac
     4741 ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt
     4801 tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc
     4861 atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag
     4921 cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc
     4981 agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg
     5041 aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg
     5101 caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc
     5161 ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat
     5221 tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga
     5281 gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg
     5341 gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac
     5401 ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta
     5461 atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga
     5521 ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt
     5581 cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg
     5641 cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt
     5701 ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc
     5761 caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac
     5821 attccatttt atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt
     5881 ccaaacattc ttgcatttca gcaatttttt tcgtcaaaag ttcactttct tcaatcaaaa
     5941 gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca
     6001 aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt
     6061 cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga
     6121 ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga
     6181 tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat
     6241 tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta
     6301 tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt
     6361 tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt
     6421 atcagcgaaa gttattatta tcgacatcgg cgggaattgc tttagagaat atggatatta
     6481 tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag
     6541 cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca
     6601 tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga
     6661 ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta
     6721 tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt
     6781 tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg
     6841 cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt
     6901 atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac
     6961 caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg
     7021 aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg
     7081 ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt
     7141 cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga
     7201 tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa
     7261 tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag
     7321 cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga
     7381 ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg
     7441 gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt
     7501 taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa
     7561 ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa
     7621 tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa
     7681 aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca
     7741 actgctaatt tccttctatc tggcggctac tattctttga cgtccgtcat ttcaaagtac
     7801 tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca
     7861 taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat
     7921 gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg cttttccata
     7981 gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat
     8041 tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt
     8101 agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc
     8161 ttttgagaag cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta
     8221 tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca
     8281 taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc
     8341 gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca
     8401 tacttaataa cagaactata gcaaaaaatc ccgtgtatca aaacgggatt agattaagtt
     8461 tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac
     8521 atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa attagttgca
     8581 ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa
     8641 agcacaagtt cagctcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat
     8701 tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa
     8761 aaagctgcac tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca
     8821 aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac
     8881 aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca
     8941 gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa
     9001 gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aagcagcagc tgaacaggcc
     9061 tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca
     9121 ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact
     9181 ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc
     9241 aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa gctggcgttc
     9301 cagatagtaa accaaaaata gcagtttaca cgtccatttc gtcagcttct ctcttttttc
     9361 atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag
     9421 tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt
     9481 ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag
     9541 ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat
     9601 ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt
     9661 ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta
     9721 gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt
     9781 agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc
     9841 gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt
     9901 gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata
     9961 cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat
    10021 gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta
    10081 aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg
    10141 ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt
    10201 gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata
    10261 tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg
    10321 ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc
    10381 attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa
    10441 tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa
    10501 aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg
    10561 cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg tgaaatcagc
    10621 gccttgacct gagtattcgc tcaacaaacg gcaaaatagt tcctcctatt ttgccgtttg
    10681 ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt tgaatcacta
    10741 tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt cttgatggcg
    10801 ttttttgaag tagatcactg ctcctccaac taaagcagta cctaccaaga tgaatacgat
    10861 gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga tcgttgtttc
    10921 atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt cattcttagg
    10981 taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tatgcccctt gtttcagccc
    11041 ttcgattgaa aatttccctt ctgaatcaga aatcaatttc acttcttccc agtcagaacc
    11101 tgtgacttca tctgaccaag catatgggta ttctcctgag gcaagatttg ctattttttt
    11161 cattaattta acagttggat cttttgctct tagaatagaa aattctgcat cggctaattt
    11221 ttcttgcgtg ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt ggacattttc
    11281 tacagtaatc gtgttatccg tagttgtatt ttcccgattg actacaccgt ttggattccc
    11341 aagattggct tcatctgcgc catggatgat accggttgtt tgttgcaatt ttccgttttt
    11401 tactataaaa gtagtttctt tttgtggatt ggttaattga taaccatctg gtgcttttgt
    11461 ttcaactaaa gtataattgc cgtctattaa tccattgaca ctgatggatc cttcagtatc
    11521 agttgtgaat gtcaacgctg actcagcagg atcatagaga gcgacaccag tgttttcatc
    11581 aaaaccggtc acgaattgat tctctgaatt tttgatctgg aattgcgccc ctgctaactt
    11641 atctacccct gaaaagttcc cagctttctg gattttgata ttgctttctt tgatgacatt
    11701 tttagcatat aggtgaatcg tgtctaaagg tttgtcaggg tctgatgaat tcgggttata
    11761 aaagggtaag cttaacacga tatttggcga cgtttcgaca ccgggctttg gtgtttctac
    11821 aaagacgtat acagcatctt tcccatcaga tttacctgga agagtcagga tagctaatcc
    11881 ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct gctggaactg ctgtgtcttt
    11941 acttggatag tacgtagcag tgatgtcata tacgttgaat tcaacgccgt tcaacggttc
    12001 gctattcgga aatgtcaatt ccgttccgtt gtttatttga ttttctggtg ctgaagtgaa
    12061 tttcaattta tgcaaattaa ccgtaacatt ttctggtgta ggtgatacgg cctagaaacc
    12121 tctgcagttc ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc
    12181 catatttttg ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt
    12241 ttttataaaa ccaagctgtc cctgttgaaa ggactagcaa caaccctaaa attgagaaac
    12301 ctccaagcat ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac
    12361 catttgtctg catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt
    12421 tgatgatttc caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga
    12481 gaatggttga ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac
    12541 ttccaaaaga caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg
    12601 ctcttgaatc atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt
    12661 catttttttt gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt
    12721 tgacaaattg tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag
    12781 tcgtcaaacg tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt
    12841 ggtaacggat cgttaatttt ttcccagcaa acgaactaag atcttttcca gaatctgata
    12901 agacaaaacc atgttttgtt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca
    12961 aaaaatcgat cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat
    13021 aggttcctat ctcgttgatg ttctcaggaa ttgttacggt gatgagataa ggaactttat
    13081 ccccatctgc aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag
    13141 gtattttttg ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat
    13201 ctgtcacagg aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct
    13261 ttatttccgc aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa
    13321 aagaagcaat gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt
    13381 cactatcact tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc
    13441 gcagcttgta aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt
    13501 ttactccttg aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct
    13561 tatcgctttc gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca
    13621 cagtcggaga acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca
    13681 cacgtacatt ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa
    13741 ataaaagaag ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat
    13801 cccacctgtc gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc
    13861 gataatcgta taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga
    13921 tccaatatct gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac
    13981 accggtaacg tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc
    14041 agcgtcatcg acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt
    14101 ggctatatca gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt
    14161 cgttagttta attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc
    14221 tgtgtaatcc atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc
    14281 tgctagcttg acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact
    14341 gagctctacc gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt
    14401 aaataaatag tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga
    14461 cgtataggtt ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt
    14521 gaaatcagtt ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt
    14581 tgattctgtt ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata
    14641 gtacttactg ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac
    14701 ttcagaaatc tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact
    14761 agctaaaaca ctcattgctg attggttctt tgtttctgtc gctgaaacag ctgtaaaggt
    14821 atatccgttg atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag
    14881 attcattatt ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat
    14941 tggtgtttgt ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga
    15001 accgtttgtt tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc
    15061 acccacatgg attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt
    15121 gactgtataa gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata
    15181 ggttggaaaa tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta
    15241 ccccgtgaat ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc
    15301 tccggcagga agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg
    15361 tgtaactgaa tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa
    15421 tgtgatagta gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt
    15481 atcaataaaa gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga
    15541 tccgttaacc gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat
    15601 ggtatgtccg ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt
    15661 ttctgaataa gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc
    15721 aatcagttga gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct
    15781 ttctatggac tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc
    15841 aaaatttgac gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt
    15901 aaaaatcaag atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt
    15961 gagtctagaa acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat
    16021 cattttcatc cctcctctca ataaaaaatc atcaaataat cctttacacg ttattctaaa
    16081 tttaattttt attattttat tgatgtatag aaagtttggg gctttttttc tctttttatt
    16141 ctaaggttat tttagtcttg tcctttaata caaagatttt atttttaaat aaaaaatact
    16201 ctttttttac gattattgaa aaaaaaagat aaaacttgat taattgaagt attgcttaaa
    16261 tttggaagct atgagg
//
LOCUS       DACEND010000058        16195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEND010000058 DACEND010000000
VERSION     DACEND010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.059"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW746_001548"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW746_001548"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1078451.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW746_001549"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW746_001549"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1078452.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IW746_001550"
     CDS             complement(1459..2235)
                     /locus_tag="IW746_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1078453.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IW746_001551"
     CDS             complement(2390..3712)
                     /locus_tag="IW746_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078454.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="IW746_001552"
     CDS             3887..4474
                     /locus_tag="IW746_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078455.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGFLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW746_001553"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW746_001553"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1078456.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRC
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="IW746_001554"
     CDS             7230..7508
                     /locus_tag="IW746_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078457.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="IW746_001555"
     CDS             7605..8426
                     /locus_tag="IW746_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1078458.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="IW746_001556"
     CDS             complement(8711..9721)
                     /locus_tag="IW746_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1078459.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="IW746_001557"
     CDS             complement(9733..10686)
                     /locus_tag="IW746_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078460.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="IW746_001558"
     CDS             complement(10676..11746)
                     /locus_tag="IW746_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078461.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="IW746_001559"
     CDS             complement(11752..12795)
                     /locus_tag="IW746_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078462.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /locus_tag="IW746_001560"
     CDS             complement(12796..13737)
                     /locus_tag="IW746_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078463.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="IW746_001561"
     CDS             13821..14168
                     /locus_tag="IW746_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1078464.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="IW746_001562"
     CDS             complement(14238..15899)
                     /locus_tag="IW746_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1078465.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4580 a         3329 c         2897 g         5389 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc ttcttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata acaacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcagaa taattaatat tttctgaagt tgtttaagag
    15961 tttaatgaat aattattaaa aaatcaacac tttttttgtc tttttcataa aataatgatt
    16021 ttttcgtgag gtttcaagga atttttcaga aaaaatagct ttgtacaagg ttttccatgg
    16081 tcttccaaaa gccattaaat aattttcgtt atattgtcta ttattgccat acaaagataa
    16141 aaaagaataa acaaaaagaa actgtgacaa aagtcttgtc acagtccctt atctg
//
LOCUS       DACEND010000059        16181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEND010000059 DACEND010000000
VERSION     DACEND010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.060"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..70)
                     /locus_tag="IW746_001563"
     CDS             complement(<1..70)
                     /locus_tag="IW746_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1078466.1"
                     /translation="MANKAELIEKVASATDLTKKDAT"
     gene            complement(313..1623)
                     /locus_tag="IW746_001564"
     CDS             complement(313..1623)
                     /locus_tag="IW746_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1078467.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(1805..3034)
                     /gene="rpsA"
                     /locus_tag="IW746_001565"
     CDS             complement(1805..3034)
                     /gene="rpsA"
                     /locus_tag="IW746_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1078468.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3169..3849)
                     /locus_tag="IW746_001566"
     CDS             complement(3169..3849)
                     /locus_tag="IW746_001566"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1078469.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3898..4533)
                     /locus_tag="IW746_001567"
     CDS             complement(3898..4533)
                     /locus_tag="IW746_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1078470.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4599..6002)
                     /locus_tag="IW746_001568"
     CDS             complement(4599..6002)
                     /locus_tag="IW746_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1078471.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(5989..7020)
                     /locus_tag="IW746_001569"
     CDS             complement(5989..7020)
                     /locus_tag="IW746_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078472.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7051..7272
                     /locus_tag="IW746_001570"
     CDS             7051..7272
                     /locus_tag="IW746_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1078473.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7437..8174)
                     /locus_tag="IW746_001571"
     CDS             complement(7437..8174)
                     /locus_tag="IW746_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1078474.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8327..9667)
                     /locus_tag="IW746_001572"
     CDS             complement(8327..9667)
                     /locus_tag="IW746_001572"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1078475.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9904..11427)
                     /locus_tag="IW746_001573"
     CDS             complement(9904..11427)
                     /locus_tag="IW746_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1078476.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(11805..12416)
                     /locus_tag="IW746_001574"
     CDS             complement(11805..12416)
                     /locus_tag="IW746_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1078477.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12539..12664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12746..13462)
                     /locus_tag="IW746_001575"
     CDS             complement(12746..13462)
                     /locus_tag="IW746_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1078478.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13468..14037)
                     /gene="scpB"
                     /locus_tag="IW746_001576"
     CDS             complement(13468..14037)
                     /gene="scpB"
                     /locus_tag="IW746_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1078479.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14021..14818)
                     /locus_tag="IW746_001577"
     CDS             complement(14021..14818)
                     /locus_tag="IW746_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1078480.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMVIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(14788..15159)
                     /locus_tag="IW746_001578"
     CDS             complement(14788..15159)
                     /locus_tag="IW746_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1078481.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15178..16065)
                     /gene="xerD"
                     /locus_tag="IW746_001579"
     CDS             complement(15178..16065)
                     /gene="xerD"
                     /locus_tag="IW746_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1078482.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4549 a         3509 c         2735 g         5388 t
ORIGIN      
        1 cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt aattctgctt
       61 tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata tcatttagtt
      121 aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc aatattctgt
      181 tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc acatgctttt
      241 tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa aaacgtcgat
      301 atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc cttcaaaagt
      361 aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca tcaattcctc
      421 ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag cataaaaaat
      481 cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat ccataatgat
      541 atcattcaac acagccgaag agatacgaag attctggttc atacttactt gttcgatcat
      601 ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga ttggagcgta
      661 atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg tattggtttc
      721 tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag cttcatgggc
      781 gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca gtaccatcaa
      841 aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt tttcagtatt
      901 ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga atttctgacc
      961 actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa tatccgaaac
     1021 aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa catttgggcg
     1081 accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt cagagaaata
     1141 cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg aaacaggata
     1201 aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt ctggattatc
     1261 tactttattg actgctaaaa tcactggttt attacttctg tataaaattt tcgcaaccat
     1321 ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta catctgcttc
     1381 ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat cgccaagatc
     1441 aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt ttgcataaat
     1501 acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac cagcgatgcg
     1561 gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg ttggatttgc
     1621 catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg cattgccaac
     1681 tttcaagcat agattgtcaa ttaaacgaag tatttttatt ccttattgaa atactaatcg
     1741 cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac agatacaaaa
     1801 tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc aacgcatcac
     1861 ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa gactcaacat
     1921 tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc aatgcaatac
     1981 gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct tcatgaagca
     2041 cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact agcccttcta
     2101 ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact gttccgtcta
     2161 atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc tgtgtgtctt
     2221 tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg acatttacat
     2281 catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa atctctgaga
     2341 catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg
     2401 caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct tttttctttt
     2461 cttctctttc gttttccact accgctttat gcgataagat caaacgattt tctgatggtt
     2521 cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa
     2581 aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca acgactaaac
     2641 cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg aaatctcttt
     2701 cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtagg tagctgccgt
     2761 tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca ccgactttta
     2821 ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga acgactcctt
     2881 caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc
     2941 ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct tccattgact
     3001 ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta accaacattc
     3061 tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat aaagcgattt
     3121 cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt aaaaaagaag
     3181 ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa tcgatttgcc
     3241 agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt cccttgttga
     3301 atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc
     3361 tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg ctactaaaaa
     3421 gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt ccatgacaac
     3481 gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga cttttccatg
     3541 tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta aagtcacatc
     3601 ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga taggatactt
     3661 tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg taatagctaa
     3721 ataagttact gcgcgataca tggcaccggt atctgtataa atataattca attcttttgc
     3781 taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg caatactgat
     3841 ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg gctattatta
     3901 tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta agccattcaa
     3961 ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg gaccttcgcc
     4021 tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg tttctgctgc
     4081 attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg gtgttgtctc
     4141 tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag attcttccgc
     4201 gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg tacttgtaga
     4261 actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat tgttgcttga
     4321 tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga ccagtaaaat
     4381 caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag aacttcttga
     4441 tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg tttcgttatt
     4501 ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac tctatcgtgt
     4561 gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat gtttttttta
     4621 aataaattta gtaaaatcgt ttcccaatga agttgttcag tcttagaaaa aagcttcctc
     4681 tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg cttttcggag
     4741 agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc attaatatag
     4801 cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag ccgttccacc
     4861 caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg cagctcatca
     4921 aagggagctt gctttgtggc atattccaaa taaagctcaa aactttgtcg ctgttctcta
     4981 gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa aagaattgct
     5041 gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt ttctagactg
     5101 tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc cataccaaat
     5161 gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg aagttgtctt
     5221 ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc cctgaataaa
     5281 tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc tccttgtgct
     5341 ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac aaaaagcgag
     5401 atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa tagtacatga
     5461 cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt gaccgggttt
     5521 cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc aactccccat
     5581 tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt tttcagatgc
     5641 tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt atattgcgat
     5701 agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa agcagctact
     5761 cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga aagcagcggt
     5821 gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca aaggcttttt
     5881 cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc ctgaataatt
     5941 tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc atttattaac
     6001 attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt ctccataatc
     6061 taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat ccttatttgg
     6121 ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat catcaaaaaa
     6181 caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac ggattgccat
     6241 gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca tgctttgatt
     6301 gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg gccgtttttc
     6361 gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaacct ctgactcatc
     6421 attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag ataattggtt
     6481 agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca aactctcata
     6541 cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt tttttagttg
     6601 gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg ttgccagatt
     6661 agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg ttctgccata
     6721 gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat cagcccctgc
     6781 aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg caattttttg
     6841 caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga taaacaaaag
     6901 ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa ggagttggta
     6961 aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa aaacaatcat
     7021 attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact tgtacctgaa
     7081 cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga ttatgatgaa
     7141 gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt gatgccggaa
     7201 gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat tttattagaa
     7261 aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct aaacttgaaa
     7321 ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga aagattcatt
     7381 tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca tttatattat
     7441 ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg gtctgcgtta
     7501 gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc tccatgattt
     7561 ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc ttcttgataa
     7621 gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg aacagctgat
     7681 tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc ttttaaatgt
     7741 ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa tgaatggcca
     7801 ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt gtttccgccc
     7861 atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt ttttgctaaa
     7921 tcttgcgccc aaatactata actttctggc tctacgagag caccaggata aaaaataagt
     7981 aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc ttctttgttt
     8041 tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt ttgctgtata
     8101 tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat tagtattcgt
     8161 ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt cttaatcaag
     8221 tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc aaataaaaaa
     8281 agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat ttagcacata
     8341 aacgatacac agcttctgca taaatttcca ttgctttata catactactt aattcccatc
     8401 gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg aatgccacac
     8461 actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca gtcatgtctc
     8521 cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct agtggcacgt
     8581 ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct gctgttttag
     8641 ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata tctacaccga
     8701 tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc tcgccggatt
     8761 gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga actaattttc
     8821 ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc agacgagaaa
     8881 tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga acgacgatgc
     8941 cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt acttcagaaa
     9001 gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct ccagctttta
     9061 cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct ttttctgcat
     9121 aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct tctttttcgt
     9181 tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata aaacgaatac
     9241 gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt gccgcaatag
     9301 aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt atagttggat
     9361 cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc atatgacaca
     9421 agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag ccttctggat
     9481 ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc aaagcttcaa
     9541 tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt acggaaggaa
     9601 ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct gtcacaaatt
     9661 ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc ctccgacaca
     9721 acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca gaatgttctt
     9781 aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttgtc cacgtgctta
     9841 tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg tatttgtcta
     9901 ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata atatcgcaat
     9961 taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg ctacagcacc
    10021 cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag attgataagc
    10081 agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca tgattcccat
    10141 tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga tatagaaaat
    10201 aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt cgccccaatg
    10261 taagatggtg tctgtgatta atccattgtt cataaccact tggataccac gagcaacagc
    10321 tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag agacaaattc
    10381 agactctttc atgccataag tgattgctac tacgatggac attactaaga ataacatagt
    10441 aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa ccgtacctag
    10501 taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat tcgcattcaa
    10561 ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga acaatacatt
    10621 aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa tatcgaaatg
    10681 cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt tttcgatctt
    10741 tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga acatcaagaa
    10801 tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta cgcctgttgc
    10861 aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata cgattgctac
    10921 agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag catagaatgg
    10981 aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca gcatcaatac
    11041 tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa tcccggcatc
    11101 tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa tgaagaaaga
    11161 gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac ctagcatccc
    11221 attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta ctcgttcata
    11281 tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg gtataaacca
    11341 agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg aaggcatttg
    11401 aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt tagagacaac
    11461 aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata ataacacgca
    11521 ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct tttttcaggt
    11581 ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac aaacattttc
    11641 gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt tatgcataaa
    11701 tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt cttgtatttt
    11761 tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt gactgctgta
    11821 tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg cgcatgcagc
    11881 actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac gatcccaatc
    11941 agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc taaaaacatt
    12001 aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt tagagcgatg
    12061 gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt tacactaaag
    12121 aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt gtctcctacc
    12181 aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa gaacgctgtt
    12241 gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat atcactaaaa
    12301 tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg taaaactaat
    12361 gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt cttcatttga
    12421 aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc aaatgaaaaa
    12481 actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt caaataagct
    12541 ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc agctattttt
    12601 ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc ccctgaagac
    12661 gaaccttata taatttttgt ttacatattc attatacata tctattcaaa aaacgcaaca
    12721 tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg ctgacttctt
    12781 ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga tctccgtatt
    12841 tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt ttcacttgat
    12901 gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta ggatccactg
    12961 ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt ccttttgtca
    13021 aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa actttatcca
    13081 cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc agtagcaaga
    13141 tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt accatcggca
    13201 aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata actcctttag
    13261 gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca tccacttcca
    13321 cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg tttactttta
    13381 cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca gcgtgggcaa
    13441 ttactttctg caatctctcc attacaatta ttctccttta ttcattccat caaagaatag
    13501 atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag gcagttcttc
    13561 taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata atatcggccg
    13621 acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta gacgctgtac
    13681 cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga ttggctgttt
    13741 ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg atgtcccttg
    13801 cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat aatggctttc
    13861 cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt ttaattcttt
    13921 cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt cttcattact
    13981 gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt tcgtcatgtt
    14041 cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt tgttgcgcaa
    14101 tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca acttcacttt
    14161 tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt tttatacgct
    14221 cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc gttttttcca
    14281 ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc aaatcgattt
    14341 ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg atgttcatag
    14401 gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt tcagctgcat
    14461 atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga tcttcttccc
    14521 ataattcttc atcgtctgtg ccgaattctt ggacaggaag caatgtcttg cttttgatta
    14581 ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg agtgttttca
    14641 ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata tcatatatat
    14701 cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg aatacatcta
    14761 atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg ctagccattt
    14821 tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt acttttcctg
    14881 taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat tcaaagaaat
    14941 gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc cttgaatgtt
    15001 ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt cgtacgtatc
    15061 aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca atagtcccat
    15121 agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt cctatgacta
    15181 agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga tatgtgtata
    15241 aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc gcaaatcagc
    15301 tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg ttacttcttt
    15361 gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt gccgagaaag
    15421 acctttccca tgattattga caaatacatg catttcagat gcgtccttta caagtaatgg
    15481 acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta aaggaatgat
    15541 ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat gaagatctcc
    15601 caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga cttccaagat
    15661 tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca gcttttctac
    15721 ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat cgatatgttg
    15781 catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc ttctgagact
    15841 tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact tctccagaaa
    15901 acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct cctgtagaaa
    15961 atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg acaatccccg
    16021 ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa tccccctcat
    16081 gcctctctat catatcagat accgcttcat tctgatagtc tttttccata atctgagcaa
    16141 agaaaaagcc gtctagacac tcagacggac tggtttcttt t
//
LOCUS       DACEND010000060        16133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEND010000060 DACEND010000000
VERSION     DACEND010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.061"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(182..601)
                     /locus_tag="IW746_001580"
     CDS             complement(182..601)
                     /locus_tag="IW746_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1078483.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IW746_001581"
     CDS             complement(603..1187)
                     /locus_tag="IW746_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1078484.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IW746_001582"
     CDS             complement(1234..2709)
                     /locus_tag="IW746_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1078485.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IW746_001583"
     CDS             2890..3201
                     /locus_tag="IW746_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078486.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IW746_001584"
     CDS             complement(3316..5652)
                     /locus_tag="IW746_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078487.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IW746_001585"
     CDS             5794..6462
                     /locus_tag="IW746_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078488.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IW746_001586"
     CDS             6465..7508
                     /locus_tag="IW746_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1078489.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IW746_001587"
     CDS             complement(7745..8626)
                     /locus_tag="IW746_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1078490.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IW746_001588"
     CDS             8785..9513
                     /locus_tag="IW746_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1078491.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IW746_001589"
     CDS             9669..9986
                     /locus_tag="IW746_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1078492.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IW746_001590"
     CDS             9979..10587
                     /locus_tag="IW746_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1078493.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IW746_001591"
     CDS             10603..11433
                     /locus_tag="IW746_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1078494.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IW746_001592"
     CDS             11571..12059
                     /locus_tag="IW746_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1078495.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IW746_001593"
     CDS             12110..12982
                     /locus_tag="IW746_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1078496.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IW746_001594"
     CDS             13158..13520
                     /locus_tag="IW746_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1078497.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IW746_001595"
     CDS             13529..14281
                     /locus_tag="IW746_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078498.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..16097
                     /locus_tag="IW746_001596"
     CDS             14295..16097
                     /locus_tag="IW746_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078499.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5017 a         2810 c         3306 g         5000 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atgcttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctggt ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caa
//
LOCUS       DACEND010000061        15916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEND010000061 DACEND010000000
VERSION     DACEND010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.062"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            304..933
                     /locus_tag="IW746_001597"
     CDS             304..933
                     /locus_tag="IW746_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078500.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1057..2166)
                     /gene="ugpC"
                     /locus_tag="IW746_001598"
     CDS             complement(1057..2166)
                     /gene="ugpC"
                     /locus_tag="IW746_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1078501.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2375..2797
                     /gene="mgsA"
                     /locus_tag="IW746_001599"
     CDS             2375..2797
                     /gene="mgsA"
                     /locus_tag="IW746_001599"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1078502.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3120..3656
                     /locus_tag="IW746_001600"
     CDS             3120..3656
                     /locus_tag="IW746_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1078503.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3696..5459)
                     /locus_tag="IW746_001601"
     CDS             complement(3696..5459)
                     /locus_tag="IW746_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078504.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5462..7177)
                     /locus_tag="IW746_001602"
     CDS             complement(5462..7177)
                     /locus_tag="IW746_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078505.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(7956..8180)
                     /locus_tag="IW746_001603"
     CDS             complement(7956..8180)
                     /locus_tag="IW746_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078506.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            8380..11148
                     /locus_tag="IW746_001604"
     CDS             8380..11148
                     /locus_tag="IW746_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078507.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11229..11648
                     /locus_tag="IW746_001605"
     CDS             11229..11648
                     /locus_tag="IW746_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1078508.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            11660..12130
                     /locus_tag="IW746_001606"
     CDS             11660..12130
                     /locus_tag="IW746_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078509.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            12147..12926
                     /locus_tag="IW746_001607"
     CDS             12147..12926
                     /locus_tag="IW746_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078510.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            12913..13734
                     /locus_tag="IW746_001608"
     CDS             12913..13734
                     /locus_tag="IW746_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1078511.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            13751..14800
                     /locus_tag="IW746_001609"
     CDS             13751..14800
                     /locus_tag="IW746_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1078512.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            14813..15817
                     /locus_tag="IW746_001610"
     CDS             14813..15817
                     /locus_tag="IW746_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1078513.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         5071 a         2815 c         3221 g         4809 t
ORIGIN      
        1 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
       61 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
      121 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
      181 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
      241 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
      301 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
      361 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
      421 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
      481 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
      541 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
      601 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
      661 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
      721 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
      781 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
      841 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
      901 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
      961 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
     1021 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
     1081 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
     1141 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
     1201 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
     1261 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
     1321 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
     1381 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
     1441 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
     1501 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
     1561 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
     1621 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
     1681 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
     1741 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
     1801 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
     1861 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
     1921 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
     1981 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
     2041 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
     2101 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
     2161 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
     2221 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
     2281 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
     2341 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
     2401 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
     2461 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
     2521 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
     2581 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
     2641 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
     2701 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
     2761 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
     2821 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
     2881 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
     2941 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
     3001 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
     3061 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
     3121 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
     3181 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
     3241 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
     3301 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
     3361 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
     3421 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
     3481 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
     3541 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
     3601 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
     3661 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
     3721 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
     3781 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
     3841 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
     3901 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
     3961 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
     4021 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
     4081 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
     4141 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
     4201 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
     4261 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
     4321 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
     4381 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
     4441 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
     4501 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
     4561 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
     4621 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
     4681 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
     4741 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
     4801 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
     4861 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
     4921 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
     4981 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
     5041 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
     5101 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
     5161 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
     5221 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
     5281 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
     5341 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
     5401 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
     5461 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
     5521 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
     5581 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
     5641 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
     5701 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
     5761 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
     5821 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
     5881 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
     5941 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
     6001 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
     6061 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
     6121 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
     6181 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
     6241 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
     6301 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
     6361 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
     6421 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
     6481 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
     6541 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
     6601 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
     6661 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
     6721 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
     6781 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
     6841 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
     6901 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
     6961 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
     7021 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
     7081 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
     7141 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
     7201 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
     7261 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga
     7321 ctattttttt taaaaaaaag aaaatgagaa aacatgccag tctcttcatt cataggaacg
     7381 tctattttac ttaatttttc tataaatgaa ttctgcataa aaaatatatc aagtaaatgt
     7441 ttatagatga aactctattt acttcttttt taatttgttc ttgcaatctt ttttgctacg
     7501 taatttttgt aaaaaagtgg gttgagagaa atctaattga ttttcaaaga aggagtatga
     7561 aaatgagaaa aatatttatt cgtttattgt gggtactatc ttagaggaat aaataatagt
     7621 ggagaaaact atggtttggc gcagattcta tcaggtttga tggaacgaag acaacctccc
     7681 gacatcaagt caaaagctac ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta
     7741 ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta
     7801 cgtaatcaat aaaaaggatg gtgacaacag tacctcttta ctctctgaaa gcatcataac
     7861 tcgtttttcc tcattatgcc aataaattgt tgttatacca catataagga tttgtaatag
     7921 ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt ttgtatggaa ggatttatag
     7981 aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt cggctatata ttgcgtatct
     8041 acgccaataa aacgagtttg ttctgtcata gccatataaa gcaaaggcaa aaatacaact
     8101 acagcgagat aagatccgat gatttctcga aaagttttcc aggaagtatg tttcctgttt
     8161 ttatatttat ccgatttcat tgaatagtca tagcctcctt attctttcat atgttgaaat
     8221 tccagtataa aaactggaag gaactgtacg ttttttagaa taaaaattat aagggataag
     8281 taggctgctg tctataataa gatatatttt tcaattcttt attaaagatt ttattgacaa
     8341 aatgaatccg ttttctatga tggaggaggg gtgaataagt tgttgaagaa ggaaatttta
     8401 tgttacttag agaaccagac agcctttttc gatttagaac atatgaacga ggtctttaca
     8461 gcacaaaggt tagctgatca ttttggggta aaaagaaaca cagtcagtca ttatttgaac
     8521 cagttaaatg atgagcaact actagtcaaa atcaattctc gaccagtgat ttatttccac
     8581 aaagaagctt ttgaaaaaca attttttaag ttacgcacga atttttattt ttctatcgat
     8641 gatctcaaag aggaacagcc ttttttcgcg caaccaaaag acttattctc ttttatgatc
     8701 ggatacgacg ccagtttgaa agaaagtatc gaacaaatca aaacagctct ttactatcca
     8761 gatggcggat taccactttt gattacaggc gaaagtggca caggtaaaag ttatttagtt
     8821 aatttggtct atcagtattg tctgcttcat gatctgctag aagattctgc accattcatc
     8881 acttttaatt gtgcacaata tgcagataat cctgaacttt tgactagcaa tttgtttgga
     8941 catgttaagg gagcgtacac tggtgcagaa gaaaataaaa aaggggcttt tgaagcagca
     9001 gatggtggga tcttgttttt agacgaggta catcgcttaa caccagaagg tcaggaaaaa
     9061 ctgttcacct atcttgatca aggtgtgatt tatcgtatgg gagaacctaa tttgtcacgc
     9121 cggatcaaaa cacgtctctt ttttgctacg actgaagaga ttactagtca tttcttaaca
     9181 actttcattc gaagaatacc tatccgaatc gaactacctg cacttaatca gcgtagtcgt
     9241 aatgaacggc tagaactgat ttactctttt tttctcgaag aacaaagaaa aatggggagc
     9301 cctttgtcag tcagtggacc agctttgtca cttttaacaa gtcagaaatt atccggaaac
     9361 ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa aagcatatgc tgaacagcgt
     9421 gaacagcctg aaatccatgt cacgatccat catttgcaaa aagaactact tgcgcagcct
     9481 attactaaaa atacagcaca gcaaggaatc tatattacgc aagaccagac cttagagcaa
     9541 ttaatggaga agcaacagcc cgaacagcag cgaatcgtta aaagttttga gcaaatccta
     9601 attgatttca agaaaaataa ctgtctgcta tattcttgcg aaggaaaatt aaagcaagaa
     9661 gtgattcaat tatttgattt tttgctgttt gagaccagta gacaagaaaa acacgagctg
     9721 ctggtttatt ggacacaatc tatccgagag acctttcgac aaatggaaac tgcctatcaa
     9781 atcaaattcg atggaaacag tgtctatgcc ttgagttact atctctatca gcgaagcact
     9841 atcaaatggt taccggaaga acatgatatt atccaactaa tcaaagaatt agaacaacaa
     9901 gtttctcagt cttatccatc tagttatcac tatgtccaac gaattctgga attaagtaag
     9961 atcaaattag atattgaaga cacaagtatg gatcggattt tactgacgat ctatctaaaa
    10021 aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga tgattgctgc tcacggttat
    10081 gcaactgcta gcagtatggc aaatgtagta aatcgtttat tacaagagga tctatttgaa
    10141 tcatttgata tgccactaga tgtcacacca cagcagattg cttcagaaat ccttgattat
    10201 tgcgaaaaca gcgatgtttc aaatggattg atcatattag tagacatggg atctctgaaa
    10261 gaaatccatc agttttttaa aaaacagtta tccgtaccgc ttttgatttt gaataatgtg
    10321 acgacacctt tagcaatcac agtaggggaa tgcctgcaaa aaaatgtatc attagaagag
    10381 atagcagaag agactgttcg acagatccag ccagaatggc gactgctcta tccagaggaa
    10441 aacaaaccca aagcactcat tactacttgc ttcactggaa tcggtacggc ggtccactta
    10501 agtgaactgc tagaaaaaag cttacctacg gcttgtcagt tgaaaatcat cccatatgaa
    10561 tatcagcaat taaaagataa gaaaaacagt gacccattat tttccatata cgaagtcgta
    10621 ggaatgattg gcaccacaga tcctgatatc accaatatcg actatctttc attagaagac
    10681 ctcatctcgg gggaaaagat gtctgtacta gctgaatggt tggacactac tatgaatgct
    10741 tcagagaagg aaatattcaa tcagcgcatc attcgtaatt tttcattgga aaaagtactg
    10801 gattccgtga ctattctaga tacagagaag gtgatgggag aaatcgatca ttttatgaga
    10861 gaattagagg tgcagttgaa tcgaagcatc agcaatccca aaaaattagc gttgtatgtc
    10921 catgtgagct gtctcattga acgattgatc cgacaaatgc caatcgaaac ctatcaagga
    10981 ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg ctattaaatc tgcgtttagt
    11041 gtcattgaag agcattatag tgtcatgatc cccaattctg agattgccta tatctatgat
    11101 attttgagtg aagatacaga gtttctctcg atagaagaag aattttgaac ataagcaagt
    11161 tgttgccact aattttggca tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag
    11221 atgaaaaagt ggatagagaa attatcttag cttcccatgg aaaatttgct tcagggatat
    11281 tagattcatt agagctgatc tatgggaaaa atcattctat tactgtattc gattgctata
    11341 ctgatgagaa ttttgattta gcggaaacag taaggcagct ttttatgagg tacaaagata
    11401 aagaaatcat tgttctgacg gatctttttg gcggaagtgt caacaacgaa ttcttacaat
    11461 atatcaatcg ggatcatgtc tatctcgttg ccggattgaa tttgccacta ttgattgagc
    11521 tagtatctcg gttggacgct gaagtagaga caactgtttt gatccagcag gtgctaactg
    11581 attcaaaaaa aatgatccag ttttgtaacg agcggctcaa tcagaaaata gaagaagacg
    11641 aattttagga ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt tattacacgg
    11701 acaagtagct ttttcatgga cacaaacaat aggcgcagac tgtattttga ttgctaatga
    11761 tgatgtacct acgaatgaga tacgaaagac aacgatcaaa ttagccaagc cacaaggagt
    11821 caaactagtg atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga
    11881 taaatataag ttattcatcg tagtggaatc gatcgaggat gcatataagc tggcagaagc
    11941 gtatccagaa atcaaaacca tcaacttagg agggatcaag gcaaaacctg gtactcgcag
    12001 catcggaaaa gcggtaaata ttttaccaga agaggaggaa cgcttgaaac gattaattga
    12061 caaacaaata gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg atgtaagaga
    12121 agttttgtaa gaaaggaaga acctcaatga ttgtacaagc agtcttatta ggaattgttg
    12181 catttgtcgc acaatcagaa tatgcactag ggacatcgtt gctttctcgt ccgattgtga
    12241 cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa
    12301 ctttagagtt agcatttatc ggatcgttct cagtaggtgc ttcgattcca cctgatgttg
    12361 tgaccggtgg gattttaggg acggcttttg ctattacagc tggagccggt acagaaaccg
    12421 cgcttttact aggtttgccg attgctacgt tgacattgat attgaaaaat atttacttag
    12481 ggcttttgat tccaattatg aaccataaag cagacaatta tgcagaagaa ggaaattata
    12541 aaggagtcga gcatatgcac ttgcttgccg gtatcggtct ttccttgatg cttggtgttg
    12601 tggtaatggt ttcttatatg gtgggaagca atacgatcgg aaatctatta aatatgattc
    12661 ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt gattccagct cttggttttg
    12721 cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc atattttttc ttaggttttg
    12781 ctatagcttc ttacttagag attcctgtga ccggagtagc tatttttggt gcgattttag
    12841 cagttgtggt agtcaatatt atgaatgtcc gtcaactacc acttcaaaca actgcagggg
    12901 aggtagaaga agatgaagaa ttctgagatc aaaaacagca aaataacgaa aaaagagcta
    12961 aatcacgtat tttggcgttc ttttcaaatg gagttttctt ggaattatga acgtcagatg
    13021 aatatggctt atgtttatgc aatgattccg attttgaaaa agttatatca gtcgaaagaa
    13081 gaaatggcag cagctttgaa acggcatcta gagtttttta acacgacccc acatatcgtt
    13141 acactgattt taggaatcac ggctgcaatg gaggaagaaa ataaggatga tcctgaattt
    13201 gatgtgactg cgatcgatag tatcaaaaca tctttgatgg gacctcttgc agggttagga
    13261 gattctttct tttggggaac tttgcgattg attgccacag gtgtgggtac ctctttagcc
    13321 atgcaaggaa atattttagg ccctatcttg ttcattttga ttttcaatat tccgcacatt
    13381 ctgtttcgct atctagcaac aggttgggga tatcgcttag gaacgggttt tttgaaaaag
    13441 atccaggcaa acggtatgat ggaaagtttg acattaggcg cgtctattat tggactgatg
    13501 gtcgttggag caatgactgc cacgatgatc accatcaata ttcctttaaa aatcggttca
    13561 ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa tatcttatgt
    13621 cttggagcat ttggcatagt gttttatcta ttgaagaaag aagtgaaacc attaacgatc
    13681 ttgatcggac tagctttctt tggaattttt ggttcgtgga tagggatttt ttaaaaaagg
    13741 agcagagaaa atgtttacga tgcaagatta tatctatgaa gaaaaggaag tactaagtac
    13801 gatcttgaaa aagaatgatt tttcgacaag agaacatatg aaaaaaacag tgaatttact
    13861 gatcttagca acgggttcat cttataacgc ttgtttagca gcgaagccag cattggaatc
    13921 ttatggtgat cttactgtcg atattcaaga acctttttat tttttcaatt atggaaaact
    13981 ttctccttca atcgatactg tgattgctgt gtcacaaagc gggaaaagtg cttctactgt
    14041 tgaagcagta aaaatgatac aaaaacaagg gttacctgtt gttgctatca caaatgacgt
    14101 gcaaagtcca cttgctttag aagctgcaca aattattgat ttaggtgccg gggtagaatc
    14161 agtaggcttc gttacaaaag gctattctgc gactgtttta cagttactac tcttagggat
    14221 aggaattgga ataagtaaaa ataaaatcag caaaaaaata gaacaagact acatgcaaca
    14281 gttgcgcaaa ataatcaacc atttaccagc gattattcaa aaaacggaag aattttttga
    14341 tggatatcaa agtttatttc gtttagcaca gcgtttcgtc accattggat atggtccaaa
    14401 ctggggaaca gctaaagaag cagagacgaa attgactgaa acaatccgcg ttccgtcaca
    14461 aggatttgag ttagaagctt atatgcacgg cccatacctg gaggcagatg cttcgcattt
    14521 gctattcttt attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata
    14581 tatgagtcgg tacgttggag agactctcac cattaccaca aaaaaagcaa gaaacgagaa
    14641 aacacttgga ttagcaatag aatgtgatga atatctgtct gtattagcgc ttgtcgttcc
    14701 gtttcaacta ttcgcttata aaaccgcagt agctaaaggt attgacttga acaaaaagat
    14761 ttttgaagac tttgatacag tcttgaaaag taaattataa gaggtggaaa tcatgctgaa
    14821 attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt
    14881 agaagaaatc atcgataaac tttatgcgga aaaatttgac gctgtctatt atctaggaat
    14941 cggtggaacg tatgcttcct cgatgcaagc tgtcacttat atgaatggga aaagcaactt
    15001 gcctgtcttt gtacagcatg ctgcagaata ttacactact ggaaacaaaa gattaacaaa
    15061 agattctatc gttattttgt cctctgttac cggtacaact caagaagtag tgaaagcagt
    15121 agaacaaatc aagaaagtag gagcgacact gattggattt atcgataaag cgaacagtaa
    15181 gttgtctcag ctatgcgatt ttgtggtcac ttatccagct cctggaacag aacaaatcaa
    15241 attttttatg gcagcagatc gattgatgta tttgaatgga gaattcgaag cttattcaga
    15301 atactatgaa cagctcgaac gttatttacc aacagggtta gtggaagcag agaaaaaagc
    15361 agatgcattt ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc attattttat
    15421 tggagcaggg aatcaatggg gagctgttta ttcatatgcc atgtgttact gggaagaaca
    15481 aagctggctt ccatcgaaat ctatccatgc tgccgagttt cttcacggga cgttagagat
    15541 cgtagaagaa acaaccccag tcacattatt tttaggcgaa gatgaacaga gagtgttatc
    15601 tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat tatacattga tagacacaaa
    15661 agactatcca gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt
    15721 gatgcatgta gtgacacaac gcatcgatgc tcatgtggag cagttgaact gccatccatt
    15781 agagatacgc cgctactatc gtcagtttga ttattaggat agactggata aaaagaatat
    15841 aaaaaataaa gagagcagcg ataactgctc cctttatttt tttaaaatat tggcatcaat
    15901 tacgttataa aacaca
//
LOCUS       DACEND010000062        15646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEND010000062 DACEND010000000
VERSION     DACEND010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.063"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..966)
                     /locus_tag="IW746_001611"
     CDS             complement(91..966)
                     /locus_tag="IW746_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1078514.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1048..1839)
                     /gene="codY"
                     /locus_tag="IW746_001612"
     CDS             complement(1048..1839)
                     /gene="codY"
                     /locus_tag="IW746_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1078515.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1857..3257)
                     /gene="hslU"
                     /locus_tag="IW746_001613"
     CDS             complement(1857..3257)
                     /gene="hslU"
                     /locus_tag="IW746_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1078516.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3271..3819)
                     /gene="hslV"
                     /locus_tag="IW746_001614"
     CDS             complement(3271..3819)
                     /gene="hslV"
                     /locus_tag="IW746_001614"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1078517.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3856..4761)
                     /gene="xerC"
                     /locus_tag="IW746_001615"
     CDS             complement(3856..4761)
                     /gene="xerC"
                     /locus_tag="IW746_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1078518.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4959..6335)
                     /gene="trmFO"
                     /locus_tag="IW746_001616"
     CDS             complement(4959..6335)
                     /gene="trmFO"
                     /locus_tag="IW746_001616"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1078519.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6304..8382)
                     /gene="topA"
                     /locus_tag="IW746_001617"
     CDS             complement(6304..8382)
                     /gene="topA"
                     /locus_tag="IW746_001617"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1078520.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8504..9361)
                     /gene="dprA"
                     /locus_tag="IW746_001618"
     CDS             complement(8504..9361)
                     /gene="dprA"
                     /locus_tag="IW746_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1078521.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9417..10184)
                     /locus_tag="IW746_001619"
     CDS             complement(9417..10184)
                     /locus_tag="IW746_001619"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1078522.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10177..11037)
                     /gene="ylqF"
                     /locus_tag="IW746_001620"
     CDS             complement(10177..11037)
                     /gene="ylqF"
                     /locus_tag="IW746_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1078523.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11297..11695
                     /locus_tag="IW746_001621"
     CDS             11297..11695
                     /locus_tag="IW746_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1078524.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11932..12474
                     /locus_tag="IW746_001622"
     CDS             11932..12474
                     /locus_tag="IW746_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1078525.1"
                     /translation="MNTLIAKNVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12557..13108)
                     /gene="lepB"
                     /locus_tag="IW746_001623"
     CDS             complement(12557..13108)
                     /gene="lepB"
                     /locus_tag="IW746_001623"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1078526.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13260..13487)
                     /locus_tag="IW746_001624"
     CDS             complement(13260..13487)
                     /locus_tag="IW746_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1078527.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13484..14002)
                     /gene="msrA"
                     /locus_tag="IW746_001625"
     CDS             complement(13484..14002)
                     /gene="msrA"
                     /locus_tag="IW746_001625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1078528.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14022..14735)
                     /locus_tag="IW746_001626"
     CDS             complement(14022..14735)
                     /locus_tag="IW746_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1078529.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14738..15634)
                     /locus_tag="IW746_001627"
     CDS             complement(14738..15634)
                     /locus_tag="IW746_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1078530.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
BASE COUNT         4162 a         3292 c         2733 g         5459 t
ORIGIN      
        1 tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt ggtaaacggt
       61 gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat attttgtctt
      121 gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt ttccactact
      181 gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg cttcttttgg
      241 ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta cgctatggga
      301 agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa ccaaggcatc
      361 ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta acgtcttttg
      421 atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca atggagaaaa
      481 tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga aagcaggatg
      541 tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat aatgaaccgt
      601 gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg gatacttttc
      661 taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat gttcaatgac
      721 ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt tcccttgata
      781 aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg catgtctgcc
      841 ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg atttcagact
      901 gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt gaataataac
      961 agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct ggaacaaaca
     1021 tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact cgtctttaaa
     1081 acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag attcaatgat
     1141 tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga tgccgatact
     1201 atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt caaagatagc
     1261 ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga cagccgttgc
     1321 actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca taccgacaac
     1381 cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt ccatacgaga
     1441 taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag tcgtcagacc
     1501 aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag taattgggat
     1561 attagcttca gttttcatca actgtgccac tgtatcagta taagattcag gaaattgttt
     1621 ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat tataacctag
     1681 taatactccg tcacttccta tgatataggt gttgctgtca agaataccac ctagtgtgac
     1741 agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt gctgcaaaag
     1801 tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc gattttttat
     1861 aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc atccacatat
     1921 gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa tagatcttct
     1981 agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt gtcacggttg
     2041 acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt aactgacaca
     2101 ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg ttcggtcaga
     2161 atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg gaagcgtcct
     2221 tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga agcaatgaat
     2281 aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc ttcgacaatt
     2341 ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg tgatttagaa
     2401 gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa tttgattgcc
     2461 tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat caataactct
     2521 aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa agcacctaga
     2581 gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg tgcaggtttc
     2641 ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt ttgtagttgt
     2701 tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg cggttcagcg
     2761 gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt agtggattgt
     2821 ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc tgcatttttt
     2881 ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat cgcattttct
     2941 actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc agtaaatttt
     3001 gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg tgcgatttct
     3061 gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat atcttgttgc
     3121 attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc aacagatttt
     3181 tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg tcttggtgtt
     3241 tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa tattatgatt
     3301 agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt ctttcgcact
     3361 catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa aattccctcc
     3421 tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac ctgaaacaag
     3481 aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca tagattgctg
     3541 tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt tatattcgtt
     3601 tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc cggcgaaacc
     3661 tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt tcatgacaac
     3721 agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt ctttttcgac
     3781 ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct cctaaatttg
     3841 ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt gtaaactttc
     3901 ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta gcaattcttg
     3961 gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat ggcgcagcat
     4021 gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca agacatattc
     4081 aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat catgctcttc
     4141 gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca gagcatcttg
     4201 agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga tcaacatgac
     4261 ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta aacggatgcc
     4321 tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac tatctcctgc
     4381 tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca gtctcaaatt
     4441 tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt ctttgagaag
     4501 atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc tgctatagct
     4561 aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt ccgccttcag
     4621 cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt cttcttcata
     4681 tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca aaaatttttg
     4741 ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat aatttagcac
     4801 agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta agtttgttca
     4861 caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg caagaaaaag
     4921 agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg gattgcagtt
     4981 ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc aattgcttca
     5041 tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc atagttggca
     5101 ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat acttccaata
     5161 gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc ggcgttgatt
     5221 ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt catctgacct
     5281 gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa ttctggcgaa
     5341 ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt ttctaaccca
     5401 ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg aaagcccacg
     5461 atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc ataaggacgt
     5521 ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag catcgttttg
     5581 aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt ttctttctca
     5641 aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc tttgaattcc
     5701 tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg ggacttcaaa
     5761 tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc atcatagaaa
     5821 taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg agaagtcaaa
     5881 ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc gtttatcact
     5941 gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga atcgcggtca
     6001 acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat tactaccgaa
     6061 ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact gtttcctctt
     6121 aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc agtcgatttg
     6181 actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg ccaagcagct
     6241 tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag aacctctttt
     6301 ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt gtttaccgcc
     6361 tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc ccactggttt
     6421 atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata gtctgttttt
     6481 cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca caccgatttc
     6541 ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat aaaacttccc
     6601 atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga atcctgctgg
     6661 ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt ctttttcaaa
     6721 cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt caattttatc
     6781 caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga agaattcaca
     6841 gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt gtgtcaattt
     6901 tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag gtcgccctac
     6961 cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg gtggttgagt
     7021 aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt caagttctgg
     7081 aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca cttgcatgaa
     7141 tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt gttctaatgt
     7201 cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac gtgaccagat
     7261 caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat ctggtgtacg
     7321 aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag catttttgat
     7381 ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa atgctgcggc
     7441 ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg taatcaagcc
     7501 gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta ccatcatggt
     7561 ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac ttgtagtgaa
     7621 aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt cgaagtctgt
     7681 tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt tctttttctt
     7741 accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa aattaccgtc
     7801 gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt caataataat
     7861 ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct tacgccagag
     7921 aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt gctgtgcatc
     7981 tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg cgtcttttgt
     8041 gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta ggtacgctaa
     8101 atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa ctttttgagc
     8161 tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta tagaaatata
     8221 atggggttca taattgtttt caaaatcgat acccatttta cttttcggta aatctcttat
     8281 atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa ttgtcttggc
     8341 ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat ggactccttt
     8401 ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta atcatatcca
     8461 ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct ttaaattctg
     8521 ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa tgatgacatc
     8581 cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt ccaagatcca
     8641 atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag acacatgttc
     8701 ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt ttcgggccgg
     8761 attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct tgcaagtcac
     8821 gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt gtccgtccac
     8881 catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag ccactgacga
     8941 tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt ttggctaaag
     9001 cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa gatagatcac
     9061 cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg ggatatttgg
     9121 gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta gcgtgaattt
     9181 cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc ttaatgatgt
     9241 cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt gcccattttc
     9301 cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct atttgataca
     9361 tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc tgttttttat
     9421 tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat tccttgtcgt
     9481 tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa accatatcca
     9541 ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc aatgatactt
     9601 gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg ctgaataggc
     9661 aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa tttttctact
     9721 gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat tgtctgctga
     9781 tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata aagaagttct
     9841 cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc atttttcggc
     9901 aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac ttcatcaatg
     9961 ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa catttcttca
    10021 tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc cagtgcttgc
    10081 tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc tattctctgt
    10141 atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt tttgctccat
    10201 ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat catgcacgac
    10261 taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa tatccatcag
    10321 taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat cgctgattct
    10381 tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat ctaaatggac
    10441 aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt catcttcaaa
    10501 tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta aacgaagcca
    10561 ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct tgcctactag
    10621 tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg cccgaatagc
    10681 gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg cttctttagc
    10741 cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta atgcaagatg
    10801 gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg ccagatccgc
    10861 tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca ttggattacg
    10921 agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat atttcatttt
    10981 ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga tggtcattgt
    11041 tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt tctttttcaa
    11101 tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga aagtgaacca
    11161 gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt tggtttctat
    11221 atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg aacaaaacag
    11281 aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg ctgatgtcaa
    11341 tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat tttctactga
    11401 agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct ctcatgattt
    11461 ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa gtggtgcttt
    11521 gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa ttgcaaaaga
    11581 aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa tgttttcatt
    11641 agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga tgtaagcaga
    11701 taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga taagtattct
    11761 ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa gcagtcttct
    11821 cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt catgacacat
    11881 tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat tttgaacact
    11941 ttgattgcaa aaaacgtttt tagattgccc gaaatgaatg caagattgtt gtcgacctct
    12001 gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc tgcttatttt
    12061 caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg accgatctgg
    12121 gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg ttcaggaacg
    12181 tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg cttattttta
    12241 tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat tatgatccca
    12301 gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga tttcaaagta
    12361 atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat aatggttgtt
    12421 tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt ttgattttac
    12481 gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct caacagactg
    12541 cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa tagacaaatc
    12601 ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta tcttttgaaa
    12661 ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat tgtcccgtta
    12721 gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat ttcaggtttt
    12781 tagttagata aggttcctta actactttcc cattgatctt caattgatca ttttggtagc
    12841 tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca tctgctaatt
    12901 gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca accatatcac
    12961 cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg aataaaaatc
    13021 cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca agccagaagc
    13081 gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt tttttcattc
    13141 tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa taaaaataat
    13201 tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa aactatctct
    13261 tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat atccatggac
    13321 ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct agagctgtgc
    13381 ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc atcatgtgtg
    13441 ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct tcgttcctcc
    13501 aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt tcaggatgct
    13561 ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct ggttcgattt
    13621 ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta cttttttcag
    13681 caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg tcaccgcgat
    13741 cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt tctactaatt
    13801 gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta tgtccagtcg
    13861 tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta tatcctgaca
    13921 tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag aagcagcctc
    13981 ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc cgatgatttt
    14041 ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt atttgagttg
    14101 tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc atcatttact
    14161 aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg atccaaccta
    14221 agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt atagtttctc
    14281 ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga aagacttcta
    14341 gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa atagaaatcg
    14401 ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc caaataaaat
    14461 ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat gatttgattg
    14521 actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc cgtaagagct
    14581 ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa aaatacggtc
    14641 gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt tgttttcttt
    14701 gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc tccttctcta
    14761 accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc aattgataac
    14821 ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg atgccattta
    14881 gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct acttcctgat
    14941 tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc attttttttg
    15001 tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca gtgatctcag
    15061 gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg gttttattga
    15121 gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc agtggctttt
    15181 taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc aaatcattac
    15241 cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt tcttcttttg
    15301 tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag ttttctgttt
    15361 caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga acgaatcctc
    15421 cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct atgtagtgaa
    15481 gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt agtgggccag
    15541 ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat aatgggaaca
    15601 aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctct
//
LOCUS       DACEND010000063        15578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEND010000063 DACEND010000000
VERSION     DACEND010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.064"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..1073
                     /locus_tag="IW746_001628"
                     /pseudo
     CDS             45..1073
                     /locus_tag="IW746_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1099..1539)
                     /locus_tag="IW746_001629"
     CDS             complement(1099..1539)
                     /locus_tag="IW746_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1078531.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1699..2205
                     /locus_tag="IW746_001630"
     CDS             1699..2205
                     /locus_tag="IW746_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1078532.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2373..4286
                     /locus_tag="IW746_001631"
     CDS             2373..4286
                     /locus_tag="IW746_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1078533.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4291..5292
                     /locus_tag="IW746_001632"
     CDS             4291..5292
                     /locus_tag="IW746_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1078534.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIEKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5494..5700)
                     /locus_tag="IW746_001633"
     CDS             complement(5494..5700)
                     /locus_tag="IW746_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1078535.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6346..7194)
                     /locus_tag="IW746_001634"
     CDS             complement(6346..7194)
                     /locus_tag="IW746_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1078536.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            7358..8260
                     /gene="gnd"
                     /locus_tag="IW746_001635"
     CDS             7358..8260
                     /gene="gnd"
                     /locus_tag="IW746_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1078537.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            8262..9263
                     /locus_tag="IW746_001636"
     CDS             8262..9263
                     /locus_tag="IW746_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1078538.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            9260..9544
                     /locus_tag="IW746_001637"
     CDS             9260..9544
                     /locus_tag="IW746_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1078539.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9577..10374
                     /locus_tag="IW746_001638"
     CDS             9577..10374
                     /locus_tag="IW746_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1078540.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            10388..11221
                     /locus_tag="IW746_001639"
     CDS             10388..11221
                     /locus_tag="IW746_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078541.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            11239..12096
                     /locus_tag="IW746_001640"
     CDS             11239..12096
                     /locus_tag="IW746_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1078542.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            12118..12627
                     /locus_tag="IW746_001641"
     CDS             12118..12627
                     /locus_tag="IW746_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078543.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12664..13065
                     /locus_tag="IW746_001642"
     CDS             12664..13065
                     /locus_tag="IW746_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078544.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            13177..13530
                     /locus_tag="IW746_001643"
     CDS             13177..13530
                     /locus_tag="IW746_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078545.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13564..14007
                     /locus_tag="IW746_001644"
     CDS             13564..14007
                     /locus_tag="IW746_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1078546.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            14024..15109
                     /gene="uxuA"
                     /locus_tag="IW746_001645"
     CDS             14024..15109
                     /gene="uxuA"
                     /locus_tag="IW746_001645"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1078547.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            15102..>15578
                     /locus_tag="IW746_001646"
     CDS             15102..>15578
                     /locus_tag="IW746_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1078548.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMN"
BASE COUNT         5418 a         2431 c         3302 g         4427 t
ORIGIN      
        1 cgatagaagg agaagaatac cgtatccgat acttttttca cttgatgtat tggcagtcat
       61 tcgacgaaaa tcattctgat atattatcaa gtataaaaaa taaacgtcaa ctattagaaa
      121 aatttgcgaa acatgcgcca tatacgcgta gtatagatag aaagaaatgg atcagcctac
      181 taagaatttc gttgcaacga atgaaacaag gccactcgat tacgtatgtc ccagaagaga
      241 tgaacaaatt tgtgcatcca ttgataacaa aagaagagtt tacgcattac atactagagc
      301 ctttttttga agcaaacttc tggtcgatga atcacatcac tccaacagag caagcttacc
      361 tttattttat gtttggtgta atgaacacct atttggaaga agatttgttg gatactccag
      421 ataaagtaca agaaagttgg ggctggattg aatcgtctat tgtagaaaat ttttaaaaaa
      481 acttcaagat ttgttttaca acttcagaaa aaaattatct atatgtcaat ttggcaatga
      541 tccatttgta ttcaagggtt tttgggacaa aaaagaaggt ggatgctttt ggaaaatcag
      601 cagaagacag agattttcag catatatttc cagtgatgta tccaatcatg caacagtttt
      661 ttagtggggt agctcgcaga attccagagc ttaatactta ttatgaaaag aacagacggt
      721 tgatttttca atactgtatg ttagtcaggg atatttttat aaaacacgaa caacctgtca
      781 tattctatat tcaatcaaag tatggtaaaa cacaggaatt atgggcaaag aaaaggatat
      841 tatctatgac tgaacgccca ataaaatata gtgcatccgc tgatcagtta cctgatattg
      901 tcattagcga ttatcctatc gacaaaaagg catatgatca aacgaataca cagatatttt
      961 actggaatgg acaacctaca aaaaatgatt gggcgcgtct gaaagctgaa atcataaaaa
     1021 tcaggaatga gaaaaataga tccatattgg aaaaattgga tttcgttaat taagaaaagc
     1081 agattgttta tgccaaaatt aagacattgc aaaaagatta ttttctttaa aatcgacaag
     1141 atgaccagaa agcaagagtt cgttgagctc ttgctttctt ttagttttag aaaggaacat
     1201 cctgatttta tcttcgctaa atccagtcaa aagttttgca gattggtcgt agataatcgg
     1261 ttttttcaat aaagaagggg atgtacgaat ctcaatatat agttcactca aactgagttc
     1321 atctattctg tgaacaagat ttttataact aatagaatga gtagagagca tttcggtcac
     1381 gccgttgttt gtcaatttta gtaagtggat aaattcattt ttggttagtg gttgacgaat
     1441 taagttctgt tcgataaacg gtatatcatg gacgataagc caacgttttg cgcgtcttga
     1501 agacgagcag ccagaacctg tatacaattt aatcatcata cattcctcca atagctaagt
     1561 atttatgaaa taataacatg aagaaatagt cgaaaagtgc catttagagg tgtaaaataa
     1621 gaaaatgttg tcacgtataa tggaactagc tagataagaa atactattaa atcaagaaaa
     1681 aagaaagaag gcacaagtat gtcaaacaca gccgacgcaa tagtagtgat cgatttacaa
     1741 aaaggggtat gttttgattc ttcgccgata cacgaattag aaacagtgac agcattagtg
     1801 aataaaagaa tcaaaacata cgaaacgatt gggaaaccag ttattttcgt tcaacacgaa
     1861 gatgctcaat tagtgccaca aacaaaacca tgggagctat tgtcggaatt atctattcct
     1921 gagcacagtt attttgtgag aaaaacccat gcgaattcat tttataagac taatttgagt
     1981 gttttattaa ctgaattgga ctgtcattcg atcgaattat gtggggcaca agcagagtat
     2041 tgtatagata cgactgtgaa gtttgctcat ggattaggat accagcttat gatgtgtaga
     2101 ggagcaacga caacttatga taatcctttt atgaaagcaa aggaaacaat cgctttttac
     2161 gagaaaattt gggataaacg atttctgaca tttttggagc aataagttgt ttcttcgttt
     2221 aaaaaaagca gaatggatac tggtttatcc attctgcttt attacgtaaa atgagaaaat
     2281 aatcctgttc tatttttatg ggataaacca tttatttttt gtataaaaaa tgtttgcgtt
     2341 tacatataat tctcctagaa acataaaaag gagtgaatag cttgagaaca aaacaagtat
     2401 taaagggagc gttggtcatc ttatttgctg taagtacaag cgtcccgcaa tggacagtat
     2461 atggagagag catttcttcc gaatcggaat ctcgggaggt agaaaccaca gaaactactg
     2521 atgctggaaa tgaaacgaaa tcgagtactt cacaaacaga aacaaatact gaaacaacgg
     2581 ttgagacatc tgttactagc acagatcaag aaacgaccgc taccacagat aaccaacaaa
     2641 cagaagaagc aacagctaca aaaatgtcaa acgacgaatc ctcttctgct aaagtaagtg
     2701 acaaagagaa tgggacagaa aatgatgata tcggtaaagg tgaaaatttc aatggtaaag
     2761 aatatttgaa ttacttcggt aaacagatga cagaaatact taatggacca acagaagaca
     2821 tcaagtcaaa attaatagag ttattagtca ctgagcttgc tgttaatgat tatggtgtag
     2881 ataaagagat tatcaaacat ctgattgcta cttatctatt gtacgatatc gcttttgcaa
     2941 aaaacagtgt gttgatgtcg atgcttggag gtacagatga tgggacaacg cctacagaaa
     3001 acacaggctt ctatccaaat acaaaacaac ttcatcaaac tgtactaaac aaacaagatg
     3061 gcaaaatgat ggatgtatat gcatattatg tcgatcaagg ttcagataaa acagtcatga
     3121 tccatggcgg atttcgaggg aattggaata acgggatcgt tacagaagaa tacaatgatt
     3181 tttataaggc cgggtataat ttattatttg ttgattccag agcgactggt aatagcggag
     3241 gcgattatgt gacctatgga caatatgagt cagatgacgt cctttactgg ataaatcaag
     3301 aagttcgaga gcggccatct caaaagattt tactttatgg tggttcgatg ggagcagcta
     3361 cgatgatgtc tgtattagcg aaagatattc ctgtcaatgt caaaggaatt attgaaaatt
     3421 gcggatttgc atcaattgat gaacaactac gttttacgta cagccagaca gtagcccccg
     3481 cactaccgga tgccataaaa aatcagttag acatcattgg ggatcaagag catgaagatt
     3541 tatttatggg ccttttgaaa caatactatt ttgatcagga gatgcatttg gatacaaaag
     3601 ctgctctacc tacgattgga atgtcagatt cattgcctaa attgattatc catggaacag
     3661 cagatgatgt agtacctgtg tctaacgcgc aaaaattata tgagttatca ggagggtata
     3721 aagatctttt attagtagaa ggagcagggc atggaaaggc acaggaagtt gaccatgctg
     3781 cctacacgaa acatgtaacg gactttttga aagtagtgtt ccatgatcag ataaacgtaa
     3841 agtatgtgga tgaaaacaat aaaagtctac tcaaagacca ggacgaaatc cagctttacg
     3901 gagcttatgg agaaaattat atgacagagc aaaagacatt cgaaggatac gaattagcaa
     3961 atgttgaagg accgactgaa gggattttta atgagacgat accaactatt attttctatt
     4021 acaaaaaaat cccagtagct ccaccaaaaa agcaagatcc gacagagaat gcagacaata
     4081 agggaaagaa tgaacctcga gaggatcaat caagtgatga tacgcaaccg attcagatga
     4141 cagtgaaagt acaaaaaaag acagatgcaa aaaaaagaat gttgccgaaa actggagaaa
     4201 agaaacattc gcttctcatt tattcaggtg gatttttatc tgttagcacg attatcacat
     4261 ggatttggcg aagaaagaga gggtaaatga atgaaatggt tcaagcgaat agtgattgca
     4321 gtaccagtta tcatcgtagt tattttagtc actgtatttt tgattaatca aatcacacca
     4381 aagccgatta gcttgcttgt tagaaaacaa tttgacacaa gtgaaaaaga acaagtatat
     4441 gcccgaccag atgatttcca gcaaaaaatc gaacaaatcg atatagaaaa agaaatatcc
     4501 tatccttcaa agtacaaaaa taataatatg gatatctaca caccgaaagt ggaaaagaaa
     4561 aaattaccga ttcttttctg gatgcatgga ggcgcatatg taggagggga taaaaatgat
     4621 tgtcgtgatt atttggaata tctatgttca gacacgcagc aaatcatcgt aaatatagat
     4681 tatgaacgat caccggaagc aaaacatcct acaccagtta tccaattgaa cgaagccatt
     4741 caagcagcta aaaaagagat aaaagatcaa gcagattggt cgaatatttc gattggtgga
     4801 aattcggcag gtgcacaaat tagtggagaa tatttgttag cacttcaaaa tgaagaaatt
     4861 aaaaaagctg atcatcttga tccgactttg gagaacaagc agattacgaa gtttatttct
     4921 ttgtctggtc tgttggatcc ttctgcactt actcaagtaa gtgataaaat ctctagcttt
     4981 ctttatgaaa aatgtggatg ggcttacttt gataataagg actttgaaca atcagagaag
     5041 gtcaaaagtt tagccttggt cagacatgcg gatcgttgga cgcaaaacac attcttaacg
     5101 gatggaaata caaatacgtt tacgaaacaa atggaagaag taacttctgt atttgagaaa
     5161 gcgggggtga aagtcacaaa agtagattat gcggagaaaa atgctgtttt gaaccatgaa
     5221 tatcagtttg atttcagcaa taaacaagcg caagaaacat atcaaaagtt agtaatgttt
     5281 ttcaaggagt aaagtcttat gatgcaaaag aggttgtaat ctgtcactta gctccaagtc
     5341 acaaggagca atttcaataa acaagctttt catggtttat gaatattgta acttgtgact
     5401 gaggagttag agatggcaag gggcaccttt tattcgaaat ttgggaatat cacacgatta
     5461 ttgtcacgat ctcttttacg taaattttat aagtcagatt tgttgttttg atcgtttaaa
     5521 aaaagcaaca aatccgaagc ctgcggaggc aagaagggac aaaaagccga gtggtaagca
     5581 aaagaatgct ggttccacta atattccgtt gttattgata taaggctggg aacaaagatg
     5641 aatagcaaat aaaatcaatg cactagcgaa aaaaagcaaa gaaatatgga tagctttcat
     5701 aagcagcacc tcctttttta taattgtatg ataaatagat taagaatcta taaatgtgac
     5761 aagtttgtta ggatgtgaat aaaatgatca tctagcgaac ttgaaacaaa gatggctaat
     5821 gataagcaat taatgattca gcaatcagat aggtacaaag tttaccatca tacgtctaga
     5881 ccaataatag aaaatatggg aatataatag aaaagattta ggataccccc aaaagaattt
     5941 ccccaaaaat tcgatgtatc ctaaactttt ttattttaca taacaatcat atcaactgtg
     6001 aaataaaatt acaaatgata acctaaaatg aggccaaaag ccagtgttat caaggaaata
     6061 cttaattcac caaaattttt tttaattttt tagaaaataa gaaacaaaaa taataaaaaa
     6121 aagaaaagat aaatttttta ctactgtata ctaactttgt tatcgcatct aacaagtaac
     6181 tcgaataata ccaatacaac cttctccaaa gatgcgatga catacgttct aacccaccga
     6241 ctattgatgt tctgtaccac ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag
     6301 tggctttttt tgttttatgg atttttcgct ttttctgtta tggtgctatt ctttgatttc
     6361 agaaatagta tgtcgaactt tagcgataga acgacgtgat tctttttcgt tgtaaaacat
     6421 caaaatcaca tatagagagt ccaaaatgct tagttgagaa atacgggaag ctaaagcttc
     6481 agatcggaat tctgtttctt cagaaataga aataaaaacg acatcgccaa gtttagcaag
     6541 ggaagaatta gcttggctag taataacgat tgtttttgct ccagtttttc ttacggtttc
     6601 agcaatatgg attgtttctt ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt
     6661 ttccgtcaat aaagaagctt ccatcaattg aatatggtaa tccgtgctgt gagaagttgc
     6721 gataggggaa cgcaaaaatt tatgataagc gtcagttgct acaatctctg aaccgccaac
     6781 accgaaaaaa tagacacgtt tagattcatt gataatagta gccgcttttt ttagatcctt
     6841 ttgttgtaac aacttttttg tgtcatttaa agttgaaata ctagactcga acaccttttg
     6901 cgccatagtg agctcagtat ctgttttctg aatattttcg tggatagaaa tagcagatag
     6961 atcattttct tggatcaaca tggccatttt aaaatcttta aatccattat aaccaagttt
     7021 ttttgtaaat tgaaaaaaag tagagtcagc aatgcccagt tcacttgcta aattgctaat
     7081 aggactatga cttactttgc ttggattttc taaaatataa tcggcgatag cctgttcttt
     7141 tgggctgaag tcgttgtaca ttgttttgat ttgtagtctt atggagacgc tcatgagaat
     7201 tcctcatttt cataataaga ttatcatttt ttccgttctc attaatgtta aagatacaag
     7261 gagagtagga aaaaatcaaa caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt
     7321 tttgtataat gagggtataa gaaatggagg cgctatcatg gaaattggtg taattggttt
     7381 gggaaaaatg ggattgaatt tagctttaaa cttaaaagag caccatcaca aagtacaagg
     7441 actcgacatt tcaagtacag ctgttagtca ggcaagagaa aatgacattc ctgcttatca
     7501 agagatctct gaatttctag ctacttttga gcagaaaaaa atcatctggt taatgcttcc
     7561 tgctggagag ataaccgaaa acatgttaag aaaacttttt ccaaaactcg gaccaggaga
     7621 cattattatt gaaggcggaa actcttatta taaagattca atccgtcgag cagaagagtt
     7681 tcagaaaaat gaaatcggtt acttggattg tgggacttct ggaggaatcg aaggagcacg
     7741 acatcaagcc tgcttaatga ttggaggaga taaagaaact tttctatccg tggaacaatt
     7801 attcaaagat gtagcaattg aaagaggata tctgtatgct ggtgcatccg gaagcggaca
     7861 cttcttgaaa atgatccata atggaatcga atatggaatg atgcaagcaa tcggtgaagg
     7921 attccaactt gtacaaaaaa gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg
     7981 gaatcatggt tcggtgattc gcggctggct gatggaaatc atggaagatc agttaaacga
     8041 acatcccaat ttaagtaatt atcggggaat cgtttctgca tcaggtgaag caaaatggac
     8101 ggttgaaaca gcgctagaaa tggctgttcc agttccagta attgcaacta gtttgttcat
     8161 gaggaatctt tcacaggaag aagatagttt ttcagcaaaa gtagtttcag cactaagaaa
     8221 tgggtttggc ggacatgaaa tagttgaaaa gggtgagtaa aatgccagat tatcatatag
     8281 caatcgttaa ttccagcagt ttcggaagag tctttccaca ccacttggaa agattaaagt
     8341 cagttggaga cgttcatttc tttactgttg atcaagagat tccaggaaaa gaactagcgg
     8401 agttacttaa aggattcaat atcattattg caagtgttac gccatttttt cgacgagatt
     8461 tttttgaaaa caaagatgaa ctgctattga tttcccgaca tggtattggt tacaacaata
     8521 ttgatttaaa agcagcaaaa gaacaccaga caatcgtatc tatcatacct gctttagttg
     8581 aacgaaatgc tgtagcagaa aataatataa ctaatttgtt gtccatgctt cgacgcacga
     8641 cacaagcaca agcaagagtc aaggacgatc gatgggaaga tcgggcggag ttcgtcggta
     8701 ggacgctgtt taacaaaaca gttggtgtga ttggtgcagg aaataccgga agcggagtaa
     8761 tcgaaatttt gcgtaacggt tttcgctgtg atgttctagc ttatgatcct tacaaatcag
     8821 atttatacat cgaaagcttt ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat
     8881 cagatatcat ttgtttatgt gcgaatttga cagaagagaa ctatcacatg atttctgata
     8941 aagaagtcga tcagatgaaa gaaaatgttt atctatctaa tagtgcaaga ggcgctctag
     9001 ttgatgaaga agcgatgatt aatggtctga aaagcggaaa gatcgcagga tatgccacag
     9061 atgttttaga agaagaaccg ggacgtcaag atcatccgta tttagcgttt gaacaagtag
     9121 tgattacgcc acatatatcg gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat
     9181 gtgtcagaga tgtcgaacag atcagtcagc accttcttcc tgaacgttct gtccagacaa
     9241 agagcaacta tataaaagga tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg
     9301 ccctcaattt tatttatatg aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc
     9361 atacggcgcg aaaagagtac tggtcaccca cggaacggta tcatgggaaa aggcgctacc
     9421 taaattagta tttttgaatg acgaaacgat tcaatttttt tatcatcgtt attctggaga
     9481 atgtagttat gctgaagcca gacgaatagc tacaatcatc aaaaaaatga aatcgatttc
     9541 gtagttggcg ttggcggagg aaaactgacc gatctagtgg gttatagtgc caatcttgca
     9601 agaatcgatt tcggagtcat ccctacttta gctagtaatt gtgctccatg gacacctctt
     9661 gctgtcatgt ataaggagaa tggacaagca gaagggaaaa cagaacactt ttacagacaa
     9721 gctgcttttt ttataacaga tccccaatta gtgattgatt cccctgttcc atatttcatt
     9781 gccgggttag cagatacact agcaaaatgg tatgaatctg agctcatctt agaacaagat
     9841 tttttgcaag atgaatcatt tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa
     9901 gagattttga atcattctgc taaagcaatc caagatatgg cacaaagaaa actgacatcg
     9961 gagttcaaac agctttcaga gattatcttt gctgttgctg gtttagttgg aggattagga
    10021 gataaatacg caagaaatgt ggcagcacat gctatgcatg acgctatcag taaatatatt
    10081 ccggaaagcc atcgttttct acatggagaa aaagtagcgt atgggatttt ctatcaactg
    10141 gcactagaag gtcgctggga aaaaatagac gagttatatc ctttgtatcg ggaattagaa
    10201 ctgccggttt ctctagaaca aatgggatta tttccgaaag ataaacagat aatagataaa
    10261 atggctgaat ttattgattc gaaagagaaa gtgcatttga ttcctataga ggtaacacag
    10321 aaatcactga tacagtcaat gatggaactg gaaggttata tcagaaagat ctgaaggagg
    10381 aggacacatg gaaacattaa gtgtagtaca aagtttgttg attgctttat gggtagccgc
    10441 ggttatgtcc cgctggttag gtggaggagc cacgttgacc ttacgttttt ctcctttgat
    10501 gactggatta gtcgtcgggt tgatcatggg ggaagttgca caagcgatga ttgttacagc
    10561 cgctttgcaa atgatttata tgggggtttt ttctccagga ggatcaatgc cagctgaacc
    10621 atctattgca gcagctatcg cagtaccagt cgcattatta ggggatctgt cgccagaagc
    10681 agcgattgcc gttgcggtgc cagtcggatt gctcggaagt tacttgtatc agtttcgttt
    10741 tttcatcaat acttttttag gtaaatatac agacaaagct gtagaagatt tgaatacaaa
    10801 agctatggcc cgatcaatta ttctttatcc aacggttgcc tcatttttat tattcgttcc
    10861 gcttgttttc attgctttat atttaggggc gccattgatc gctgatgtaa tcaaagcttt
    10921 agagggaaca gtcgttatcc acattcttga agtagtaggt ggagggttag cggcaatcgg
    10981 tatcgcgaca actgtatacg ttatcggtcg aaaagattat ctggtctttt tcttccttgc
    11041 ttattttatg agtgttgttt tgaaatcttt agatattacg atggttactt atgccatttt
    11101 tgggattatt atcgctttga tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc
    11161 cacaagcaat aacaattcag catcttttga tgacgaagat gactatgatg acggatttta
    11221 atttaggagg aatgggaaat ggctgaaata atggataatc aaacaggtgc tcctgaagaa
    11281 atcacgaaaa aagatgtaac aaaagcctat ctgagatggc attttgcaaa cgaaattcct
    11341 cactcttttg aacgttactt ggcgccatca ctactatacg gaatgatgcc aatcttgaaa
    11401 aaactttata aagatgaaga ctctttaaaa gcagcatata aacggcagtt actgttcttc
    11461 aacactcaat taagctgggg tggcggtgtg attactggcc taatgtcttc tatggaacag
    11521 caaagagcag aagaagagta tgaagaaaaa gagatcttga tgcaggatga cttgatgtat
    11581 aacacgaaag cgggattgat gggggcacta gcaggaatcg gagatgcaat cgattcagga
    11641 accgtccaat atatttttat cgcaatcgcg gtaccttggg cacaacaggg aagtccttta
    11701 ggcgcacttt ttccttttat ctgctttggg ttgtatcagg ttttactagg ggtatttttc
    11761 gcaagacaag ctttcactat gggccgtaat gctacaggcc taaagcaaaa cactggtgtt
    11821 cagacagcga ttgaaatgct atcagtttta gggttgttta tgatgggggt gcttgcggga
    11881 aactatgtaa aagtagaatc ttcattgaaa tttgcgatat ccggacgtga gtttgttatc
    11941 caggaaattc ttgatcaaat cgtgccaggg atattacctt tagctgtcgt aatgggtgtg
    12001 tacttgttct atacgaaaaa aggattgaaa gtcacacaag cattattatg gctgactgga
    12061 atattaattg tgctagcagg aatcggtatt ttataaaaag aagggaacgt gaataagatg
    12121 ggtgtagtca atttagcgcg tgtggatgag cgtttgatcc atgggcaagt aatggtaacg
    12181 ttatctcaaa aaagtggagt gaattccatt tttgttgtag acgaagttgt agcaaaagat
    12241 aaatttatga gagatcttta caaaaatgca ggaagccgta caggacaaaa aacaattgtc
    12301 attacaccag agaaagctaa attctattgg gatgaatatc aatttaaaga atacaattgt
    12361 attttgatcg caaaaacagt ccaaacgatt tacgacttag taaaacacgg aatccccatg
    12421 gaagaattga atattggagg gattgctcaa aaaaatcctg atacagatct tttagtgacg
    12481 aaatctgttt atttaaataa atcagatgct gaaaaattga aagaaatgaa tgaacaatat
    12541 ggcgtaaaga atatttattt tcaagccact ccatctgcac ctaaaacagg attaaaagat
    12601 gtattgaaca agtttgattt gtcataaaca aatgcttaga aaaaagataa agagggcgaa
    12661 aaaatgaaga agaacgagct gtttcgtgat tgggaatttc gttatcgcta tatttaccgt
    12721 aagcgccgta caaaaaagag taaacagcgt tttctttcag cattagtatc agatatttac
    12781 agtatgagaa cagatgtgac agtcattgct tacgatacgc tagcctaccg atcaaaaaat
    12841 atttatgttg gagatatcga aaaagcagag aaagttatct gtacttatta cgatacaccc
    12901 gttcatgcat taggaagtta tttcatgttt gattggaaag accaaaggaa aaaaacaatc
    12961 tactctatat tattgtcctt cattttgttg ttcagtttag gttggtgggg aatgatgata
    13021 tataacaaaa atcctcatca cgtattcgat ctgctatccg tataaacagg tatcactgta
    13081 ctagcatttg ggagctactt tttcttattg ggaaaagcag ctcgcggatg gcctagcaga
    13141 caaactttta ttcgaaatac ttcatccata ttaacgatgc tagaaatgat ccgtacgatt
    13201 gatgatccga gtgtagcgta tgcgtttgtt gatgaaggct gttatggaga aaagggctta
    13261 gacagcgtga gatcgggtat gaaaaaagag gctattcttt tttatctaga ctctgttggg
    13321 gcagatacac cgctacagtt ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa
    13381 caagttgata agttgaaaga aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa
    13441 gcgcagttct tctatttgac caaaacagat ttacgaggga aaacatttaa ttggcaaaat
    13501 gccaatcaga ttattgctct ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga
    13561 ctaatgttgg aaatcatgat cgttacacat ggtaagttga gcgaaggact gaaagatgcg
    13621 gcggaagtaa ttgtcgggac atcgagccat attgaaacaa tgtgcttaga gtctggggag
    13681 gacatccaaa cattcggaga gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa
    13741 cccatgctag tatttgttga tctaattagc gcaagtccgt acaatcaagc gctcttggca
    13801 gtcaatgaac tgactgaaga aaagaaagca gccacttatg tcataggggg cgtgaatctg
    13861 ccaatgttgt tagaagcaat caatcatcag atgcttcaca caccgataga agaagcggtg
    13921 gaacaaattg tcaaacaagg aaaagataat ttaactgttt ggcatgcttc agcggaagaa
    13981 aaaaaattag atgaggacga tttttagaag gaaggctgaa aagatgaagt ggggatttag
    14041 atggtatggt gaaaaagggg attcgatccc attaaaatat gttcgccaaa taccgggaat
    14101 aaatgggata attggaactt tgctgactaa aatgcctgga gatgtttggg agatttcaga
    14161 aattgaggag ttgaaagcat ctgttgagag ccaaggactg gaactgcttg gaattgaaag
    14221 tgtagcgatc cacgatgcaa tcaaggcagg gacagatgaa cgagattact atattgataa
    14281 ctatatacga acaatcaaaa acttgtctcg atgtggggtt cgattgattt gttatagctt
    14341 caaaccaatt tttggatggg cgaaaactga tttgtttaat gaaaacaacg atggaagcta
    14401 ttctctttta tacgatcaga aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt
    14461 gatacacagc cagtcaaagg gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa
    14521 attcgctcag ctggaaaaac tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct
    14581 gacttatttt ttagaaaaag tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat
    14641 ccatccagat gatccgccat gggaactttt tggttttcct agagtgacga aaaatcttgg
    14701 cgacttgaag aaaattctaa atgcagtaga ttctccatat aatggactaa cgctgtgcac
    14761 aggatcttta ggggcaaatc cggaaaacga tttagttgaa atcatccatg aggtaggttc
    14821 gcgcatccat tttgtccact tcagaaacgt ggagttttta ggagaaagaa agttcaaaga
    14881 aacggcacat ccctctacag aaggctccct tgatatgtat catattatga aagcgttggt
    14941 gcaagaaggg ttcgacggtc ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt
    15001 ggctatgcct ggatatggcc tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct
    15061 atatgaggcg ataagtaagg aaaaaccact gggagagcat tatgaataaa atcaagctct
    15121 tcatctttga tatggatggc cttttattag aaactggacg tttagcttat cgtgcttatg
    15181 taaaatcagc acaaaagtat gattatgaaa tgcgtaagga agtctattat ttactaactg
    15241 gccaaacaga aatggcgata agagaacaaa tgggcatttt gtacggagaa gatgttccat
    15301 atatcaaatg gcgagaagca atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga
    15361 gagtatatac gaaaaaagga gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc
    15421 atacaatcgt ggcttcgtct aatacacggg taaaagtaga aatgtattta aaaatggaaa
    15481 atctatatgc cttgtttgat caaatcattt caggggatga tgtaaaaaaa ggaaagccgg
    15541 aaccagagat ctttttgaaa gcttgttcaa aaatgaat
//
LOCUS       DACEND010000064        15012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEND010000064 DACEND010000000
VERSION     DACEND010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.065"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(100..414)
                     /locus_tag="IW746_001647"
     CDS             complement(100..414)
                     /locus_tag="IW746_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1078549.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(428..688)
                     /locus_tag="IW746_001648"
     CDS             complement(428..688)
                     /locus_tag="IW746_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1078550.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            858..1172
                     /locus_tag="IW746_001649"
     CDS             858..1172
                     /locus_tag="IW746_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1078551.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1174..2829
                     /locus_tag="IW746_001650"
     CDS             1174..2829
                     /locus_tag="IW746_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078552.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2912..5047
                     /locus_tag="IW746_001651"
     CDS             2912..5047
                     /locus_tag="IW746_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1078553.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5222..5731
                     /locus_tag="IW746_001652"
     CDS             5222..5731
                     /locus_tag="IW746_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078554.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5728..7032
                     /locus_tag="IW746_001653"
     CDS             5728..7032
                     /locus_tag="IW746_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1078555.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7128..7997
                     /locus_tag="IW746_001654"
     CDS             7128..7997
                     /locus_tag="IW746_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1078556.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8109..8429)
                     /locus_tag="IW746_001655"
     CDS             complement(8109..8429)
                     /locus_tag="IW746_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1078557.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8583..8978
                     /gene="dhaM"
                     /locus_tag="IW746_001656"
     CDS             8583..8978
                     /gene="dhaM"
                     /locus_tag="IW746_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1078558.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8980..9963
                     /gene="dhaK"
                     /locus_tag="IW746_001657"
     CDS             8980..9963
                     /gene="dhaK"
                     /locus_tag="IW746_001657"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1078559.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            9991..10596
                     /gene="dhaL"
                     /locus_tag="IW746_001658"
     CDS             9991..10596
                     /gene="dhaL"
                     /locus_tag="IW746_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1078560.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10743..11408
                     /locus_tag="IW746_001659"
     CDS             10743..11408
                     /locus_tag="IW746_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078561.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11691..12350
                     /locus_tag="IW746_001660"
     CDS             11691..12350
                     /locus_tag="IW746_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1078562.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12427..14037)
                     /locus_tag="IW746_001661"
     CDS             complement(12427..14037)
                     /locus_tag="IW746_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1078563.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(14050..14931)
                     /locus_tag="IW746_001662"
     CDS             complement(14050..14931)
                     /locus_tag="IW746_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078564.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         5056 a         2740 c         3108 g         4108 t
ORIGIN      
        1 ttttcacaac ctttgattca cggttttcca gaagtagctt cttcgaaaaa taagccgaaa
       61 tttcttgaag ctgatcactt ctgtcgcaac ctctactgac taaggcttga tataagcata
      121 accttgggat tgcagttcaa ctaaatcaag gacacctgct ggtacaacga ttacttgttc
      181 aggtaaagaa ctttctgaaa tatgattagc acgcatagca ttggcgcaag cgtgaaaaac
      241 gactccttgc aaatctaaga attctgcatt ggcagagtct agataaccgg taatcgcgat
      301 gccatttaca gatacgacaa tcgtcgtttc tggagaagct tttatcaggt tttttacgtt
      361 tttccctgtt tctgaccatt tttctaattc atcaatatgg aaaacgactt tcattgaaat
      421 cattccttta ctttttaata atcatcttga tcgcttgttc tagtttagcc gcttgttctt
      481 ctggattact atcaggtttc tcaaagcaat ttttcagatt atctgcaacg atcatgccca
      541 ttacgcgatc aatactagaa cgaacagcgc ttaattgagt gatgacatcc atacattctt
      601 tttcgtcttc gatcattttt tggataccac gaatttgtcc ctctgtacgt ttcaaacggt
      661 tcaggatatt tttattatca atctccataa cacattctcc tctattttta taattagata
      721 atacccctta tcggtatttt atgcaaatat aaaaagtagt gaaaaaaatg aagagtcatt
      781 ctggttgaca tattaataaa gctcctgttt aatatacccc atgaggtatt tataaaaatg
      841 taaatgaggt gcagaaaatg aatttgctat ttcaatcaat aaaatcgata acagtcccag
      901 aattaaagga aaagcttttg gaaaatccag cgttacttga tgttagaaca cctgcagaat
      961 accggggcgg gcatataaaa ggagctaaaa atgttccatt acaaagtata aacagatatg
     1021 atggagacaa agaaaagacc gtctatgtga tttgtcaatc agggatgagg agtaaacaag
     1081 cagctaaaga attaaagaaa tctggttatg atgtagtgaa tgtacgtggt ggcatgaatc
     1141 aatggtttga tcgaacagta ggagggaaat aagatgaaaa tcgttattgt cggaggtgta
     1201 gcaggtggaa tgtctgctgc tacacggctt cgccgattaa tggaagatgc agaaatcgtt
     1261 gtttttgaaa aaggaccgta tgtttctttt gcaaattgcg gcttgcctta ttatctttcg
     1321 ggagaaatca gtgaacggga aaatcttctt gtccaaacgc cagaatcatt atccgctcgt
     1381 ttttgtttag atgtgcgtcc aaatcatgaa gtgacagcca tctttcccga aaacaaaacg
     1441 gtagaagtcg tacatgaggg tcaaaaacac attgaacagt acgatgcatt ggttttatct
     1501 cctggtgcaa aaccagttgt tccatcgatt ccagggataa cagaagccga caatgttttt
     1561 tctattagaa atgtaccaga tatcgataaa gtgatgcatg cattagaaaa acagccaaag
     1621 cgtgccgtga tcgttggtgc aggattcatc ggattggaaa tggcagaaaa cctaaaaaga
     1681 agaggtttag aagtcatggt gatcgaacaa gcaccacata ttcttccaac actggatgaa
     1741 gaaatggcag cttttataga aaaagaattg tctcatcaag gagtagaagt gattacttct
     1801 catgctgtcg ctggatttga agaccacggg aaacgattgc gactggatga tgggcgtacc
     1861 atccctgctg atttagttat tttatccatt ggtgttcgtc ctgataacca gctagcagtg
     1921 accgctggaa tcgaattagg catacgcggg ggtatcctag tagacgaacg ttatcaaacg
     1981 aatattcctg atatttatgc ggtgggggat gctatcgttg taaaacagca aatcactgga
     2041 aaagatgcac ttatttctct tgcttcacca gccaatcgtc aaggtagaca agttgcggac
     2101 acgatttccg gaatttctcg aagaaatcaa ggcggtattg gaacagcaat tatacgaacg
     2161 tttggaatga ctgccgcatc caccggttta agtgaaagaa cagccaaaga aaacgaactg
     2221 tcttttgaag tcattcatgt atcaggaaaa gatcatgcaa gctattatcc agaagcaaca
     2281 gatattttac tgaagttgat cttccatcca gagactggcg agatttatgg tgcacaaggt
     2341 gttggggcaa aaggtgtgga taaacggatc gatattttag caacagcaat caaagggcat
     2401 ttgacgatct tcgatttacc ggaattagaa ttgacgtatg caccgccatt tggctcagcc
     2461 aaagatccag taaacatgct aggatatgca gcaatgaaca ttgcagaagg gcttagtgaa
     2521 accgtacaat ggcatgaatt gccgacagaa ctagcaaaag gaaaaatttt attagatgtg
     2581 cgaacagcag aagaattgga aaagggcaaa ttcaaggaag ccaaacatat ccctttgaat
     2641 gaacttcggg accgattaaa tgaattagac agccaacaag aatatatcgt cagctgtcat
     2701 agcggattac gtagctatct agcggaaaga atcttgaagc agtctggcta ccacgtaaaa
     2761 aaccttgatg gtgcattttc tttatatcaa actgtccgac ctgaagaact gatatatcct
     2821 aacaaatgat actcgttaaa aagcttgcat tcgactctta aaagaggtaa actaatcggt
     2881 tccaccactt taaaaaaaga ggagaatgtg tatggtcaac acacaaaaac agttagaaga
     2941 agcatatctg aagaacgtgt atcaaaagct aattgataaa aaaaacgaac tgcgggaatc
     3001 gatggatacg gcaaaaatag aaggaaacca gtcgttgaaa gaaatggctg gcgagcttcg
     3061 attgaatttt gacagttact tagataacct ggatactttt tcgatgattg aaatgaaaaa
     3121 tagagaaatc gatcagatga atatcaaaca gaaaaacgca gaaaatcagc tagcaaagat
     3181 cgaacgttta ttgaaaagtc cctactttgg aaaagtcgtt gtagactttc tggataatga
     3241 gccaaaagaa tccttttata tcggtacagc aaatttcaca gatgatgcag atgaaaatct
     3301 tgtttatgat tggcgttcac caattgccga acttttttac aataacatga ttggtccatc
     3361 ttcttatacg gttcgtcaaa ataaaatcga tacggttatt aaagaaagac gacaatttct
     3421 tttggaaaga gatcgtttgg tcaagttttt tgatacgtct gtagctattc aagatgacgt
     3481 attattagaa gtcttaggcc aagatgagac caatgaaatg aaggctatca ctgctacgat
     3541 ccaatcggaa caaaacacaa tcattcggga tgtgaaaagt caaaatatct tggtaaatgg
     3601 cgtagcagga agtggtaaaa cctctgctat catgcagcgt attgcgtatc tgctctattt
     3661 atatcgagat acgatgacat ccgatgactt gctgattcta tcacctaatc atcggtttat
     3721 cgactatatt tcgaatgtcc taccatcatt aggtgaaaga aatccactga atctaaccat
     3781 catacagctg gtttcacagc tttcagcaga ggaaatagaa ggtgaagagg cttatttcaa
     3841 acgtatcacg ggagaaaatg tatcggagca gacagaacgt ttgcgtagca aaaagtttat
     3901 cgataatcta aaacaatcag atcctttgtt tttagatcat ccgaatttta tccgtggact
     3961 tactaaaaat ggaaaaacag tcctttctaa aaaaacgatc gaaaaaatat atgagaaagt
     4021 tcctgctcat cctaaattga tcgataggct tcaagcaacg aaaaaggcac tgatgagcga
     4081 atggaagaat catcttttga aacaagcaaa aagtcctgct gtgcagaatc aggtactctc
     4141 gttgacagaa gataggcagt tggaattatt cggtaaattg atttctgatg attcggaaca
     4201 aagtattgta gcttatgcaa ggaaactttt acaaaaaaaa tatcgaaaaa taaccagaca
     4261 aatcgaagaa atggcatggg tggatgagca tcagttgttt gaaagaattt atgaaaaacg
     4321 atatggctca gcttatgctt ggcaacccac acgtactgtt gatgaagcag tcattatatt
     4381 agctattcgt catttacttg ttgaaaaggt aaatgtacca gcatttcgct atctattgat
     4441 tgatgaagta caagattata ccctggcaca attaggtcta ttgatcgaat tatttccaaa
     4501 aactcacttt actttagtag gtgacgagaa tcaggcaatc tttaattctt ccactacttt
     4561 tgctgatatt atgcgttgtt tcgacgacta ccatctgccg atccatcggt atgatcttag
     4621 aaatagttac cgttctagcg gagcaatcac agagcttttc aaaacctatg ctgtggatca
     4681 agagaagatc gacatcgtat cgatccgacc gcaaggaaaa gaacccgaat accgctcttt
     4741 tcgatcctct gaagaattgt tgaaaatact agctgagatt ttggtttctc taaaaggaga
     4801 aaaagcggca gttatcaccc aaacagaaga ggaaacccaa acgctacaag aatcgattaa
     4861 aaaaaattca tatggattag aaaactgcca aatcattcct ttgagtctag caaaaggact
     4921 ggaatttgat catgtaatct tatatccatt tgaaaacgat ggagatgagc aaagaagacg
     4981 cagacaaatg tacacggcga tatctagagg aatgaaaagc attgtcgtat tggaaagagc
     5041 aacataatta agagataaaa aataaaaaga acaactgata aaaagaacaa aatatttttc
     5101 atttgctgat gagaaatgtt ttgttctttt tcttaggaaa aatttaggaa cactttatgt
     5161 gaaaagaaat gaaatgtatg gtaaactcct acgtcgtaac aaaaaaacgt gaggtgagga
     5221 tatgaaaaaa gtaacaagag aagaagttgc atcaattttg gtgaaagata agtatttttc
     5281 aaaaggtaat tactggttaa agatttggca aacgttagtg gctcttttag gatggatgat
     5341 cgttgttctt ccttttatat gggtcttttt cccattaacg agaccagaaa gagccaagga
     5401 tttttccttg tacgcttttt tatcagaaaa gaaaatgctg tatttcttaa tcggcttttt
     5461 tgtattgatt tttttcagtt tcctgattac ctcatttgta ttgactaggt ggaacaatcg
     5521 aaatctatac aaaaaagtaa ataaatcttt tgaatatgaa gatgaaaaat tgaaaaaaag
     5581 acaagaatta cttgaagaag tgtatacgga acgttttgga acaaaagaag agagagaaac
     5641 ggtccgtttt tattctgtct ccgaagagaa aaatctagat acgacattca ttgcagattt
     5701 gtataaagaa aatggggtgg aactgaaatg atctcaatca cccatacagg taatttattc
     5761 ttagatactt gtttgtctat tatgtatttt tttctcgttt cttatccgat tttaggcgga
     5821 tttgtatggt ttattggtgt ctggtgttat gtgtttttat acaaacataa gcaaaaagaa
     5881 tgggtagatg ttcccttgag tgtcgaaccg ttcatcacga tcatggtgcc agcccataat
     5941 gaagaaattg tgatcgaata tacaatcgag tatttgatga cgaaattgaa ttatcacaac
     6001 tatgaggtat tagtgacaga cgatggttca acggaccaaa caccagaaat attagctaga
     6061 ctgatgaaaa aatacgctaa tcttcgtgtg gttcggatcg aaaaaaataa aggaaaagcc
     6121 cacgcgttta atatcggact tgcctttgca aaagggaaat tgattttaag caatgatgca
     6181 gacacggtac cggagccaga tgcactgatt aggtatgtga attatttcat tcgccctggt
     6241 gcaagacata ttgctgcggt cactgcaaat atggatgtcc aaaatcgtac caaattgatt
     6301 gcaaaatctc aaacggttga attctcaagt atcgtgggca tcattaaacg aacacagtca
     6361 gctgttttcg gtggtcttta tgcgtacagt ggagcaaata cgatgtatcg aaaagaagcc
     6421 ttgatcgatg tgggcggatt cagacaagat cgagcgacag aagacatcag tattgcttgg
     6481 gatcatcagt taaatgactg gttatctgtt tttgctcctg agattatctt tttcatggaa
     6541 gtacctgtga cgttgaagat gctatatcga cacagaaaac gttgggcaaa aggtggaaca
     6601 gaagtctggc tgacgaattt caaaaaagtg atgctccatc cttttaaaca tattggaaga
     6661 acgatcatct tcattgatca aacattgagt attgtctggt caatattctt ttgtatttcc
     6721 gtagttcttt ttgctggatt aatcgggcat tatgtatatc aaggaaacta cgaacagatt
     6781 tatattactt ttacattcag ttttgtgttc atctgctttg aaatggtcgc aggatttttc
     6841 cagttgctag cttcgctgat tgtagatgat cgacgaagaa aattgaaata ccttcttttc
     6901 gcaccactat atatgctttt gttctggata gtgaacgcga taacgatcgt gacgactttc
     6961 attccagcag tcaaaacgat tttaggttat ggaagcggga catggaaaag cccggaacgt
     7021 acaaaaaaat aaaagatgca ccgtttttta gacaaacagc ctgttgtgtt tatggttctt
     7081 ccaaacattt ttgcttataa taagaaatta gttaaaaagt agtaaggatg ggaaaagtga
     7141 tgaaaaaaag aatgaagccg ttaatctggc caattgctgt ttccagtatt ttcttattgg
     7201 gacagacagc ttttgcggaa acttctgaac ctgctcaaac agaaccttca acaaccataa
     7261 gctcttctgg tgctgaaaac ggtgcctcgg aaacagaaat gacttccaat gtaccgacag
     7321 ataattcagg agaaacgatt tcatcttcag aagatacatc aataaatagt tctacctctt
     7381 ctgaccaaat cgatcatttg caagacttgg aaaataaaag aaaagaactc gaagaagcat
     7441 tagaagctgc aaaaaacaaa ttgaaagaag accaagcagc aatcgatcaa gatttagctg
     7501 atcttaaaaa taaagtggga aatgaccgtg cacaacttga gaaaaagctt gccgaagcga
     7561 aagcaaaagc ggaagctgtc caaacagatc ttgacgggaa gatccaacaa gcaaaagaat
     7621 tcaatcagca attaaatcag gttgctcaaa cagcttctgg aaagctaagt caaactcaag
     7681 cggatatcac agcggccagt caagcaatcc acggaaagat cagcgaagca caaacagttg
     7741 ctaatgattt tgaagcaaaa aaagctgact tagcagctag tttccaacga ataaaagata
     7801 ttttgcattc ggcaggagaa aaatcagtga cctctggaag cacgacagaa acagttcagc
     7861 aaacgacaga gccatccaaa tcaacaagtc agactttgcc aaaaacaaat gaaaaaacag
     7921 cttctgtcag ttattcctta gcaggggcgg gtgtgttagt cgcagcagct tacggaatca
     7981 tccgcaaaaa gaaataaaaa agagaggatg ggaaggtaga gtacctcgaa gttagactga
     8041 aaaatctaat tcttgagtgc cactacatgt cccatctctc tttagttatt aaaacagatg
     8101 tcccctactt atctcgtgga gacagctgtt ccactacatg tgaccatcag catgccattg
     8161 tcgctgccta atacttcata gtccattttt acgccaatga tcgcatcagc tcctaatgct
     8221 tttgcacgtt cggccatttc ggcaagtgct tcttctcttg cacccattag ttcgttttca
     8281 tagctttttg agcggccgcc aaagatattt cgcaaccccg cctccaagtc tttaaatgca
     8341 tggatcccag taatgacctc accaaaaacg atccctttat aagctactac ggcttttccc
     8401 tctacactgt tcgttgttgt tgtaatcatt cgatcactcc tctcttttaa ttaaagtata
     8461 acaaacttca agaagcacta cacatatttt cccaaaatta gaaatttatt atatacttga
     8521 attgttcatt aatgaaagcg aaaccaatcg ttagcaacta tgtaaaagaa aggaagattc
     8581 ttgtgaaaaa aagtattttg cttatttctc atagtcaaaa agtaacagat ggtattaaag
     8641 aaatgattga gcagatgcaa aaatccgaag aggtgcaaat tttttctctc ggcggaataa
     8701 atggcgagat cggatcagac ccgatgaaaa tcgtagaagc agtcaatgaa tcaagcgatg
     8761 cagctagtta ttttgttttt gcggatatcg gtagcgcggt attgaactcg gaactggcaa
     8821 aagacatgtt ggaagaagag cagcagcaac gttatcatct ggtggatgcg cctttagtgg
     8881 aaggagcttt tgcagcagcc atcactgccg gtataacaga tgatactcag caaatcttaa
     8941 cagaagcaca aaatgccgga aagaaaggat gggaataaat tgaaaaaaat catgaatgat
     9001 ccaagcaata ttgtcgaaga aatgctggaa ggattagtaa aaagctatcc agaacttgtt
     9061 catcgtgtag aatcttcccg tgttgtagcc aagaatcaga aagcggaaca agttggtttg
     9121 gtttctggtg gaggaagcgg acatgaacca tcccatgccg ggtttgtagg ggaagggatg
     9181 ttaagtgcag cggtgcttgg tgatgtgttt acttctccaa ctccagatca gattcagacg
     9241 gctattaagg aagccgatag tggaaaagga gttttgctga tcgttaagaa ttatacaggg
     9301 gatgcattga acttcgatat ggcaaaggaa ttagctgcta tggacgatat tgaagtagaa
     9361 agtgtgatcg tagatgatga tatcgccgtt gaaaatagta cgtatactgc tggaaaacgc
     9421 ggagttgcag gtactgtcct tgtacacaaa atcgtgggcg atgcagcaag aaacggtgct
     9481 tccctagctg agttgaaaga actgggagaa aaggtcgtac aggcaactaa gacgatcggt
     9541 ctagcgcttc gagcagcaac tgtaccagaa gtagggaaac caggattcga actaggagaa
     9601 gatgagatcg agtatggtgt tggtatccac ggtgagccag gctatcgtcg agaaaagatg
     9661 cagccttcta aagtactggc aaaggaactt gttactaaga ttttagatga ttactcgaat
     9721 ttaccaaaag aagcaggtgt actagtcaac ggaatgggcg ggacgccttt gatggaacag
     9781 ttcgtgttca tgaatgatgt tttagctctg ttggaagaaa gaggcgtcaa tgtagttttc
     9841 cgaaaagtcg gtaattttat gacctcacta gatatgcaag gtctttctct aacactgatc
     9901 gacttgacgg agacacaatg gaaagattct ttagaaagca atgtacagac gatttcatgg
     9961 taaaaaatct caagataaaa gggtgataat atgaaactaa ccgttcaaga aatccaagaa
    10021 tggctcacgc aatttgcaga ggcaatcaat cagaacaaac aatatctcag tgatctagat
    10081 accccgattg gcgatggaga ccatggaaat aacatgggaa gaggggtttc agcttatgaa
    10141 gaagcttttc aagccgatca tcctgaaaca atcagcgata cattcaaagt attctctatg
    10201 gcgatgatat cgaaagtcgg tggggcttct ggtccgttat acggatctgc attcatgaac
    10261 atgatgaaag cgacaaaaga ggtcgattct attgattctc aagaaaaatt aggcgaagtg
    10321 atcgagcagg gaacagcggg tattcaaagc cgaggaaagg cagaagctga cgataaaacg
    10381 atgttagatg tttggctgcc agtcacagag gcattgaagg cagggaattt aacaaaagaa
    10441 atcattgacc aggcaaaaga acacaccaaa gtcttggtag ctaaaaaagg acgagcttcc
    10501 tatctaggag agcgtgcaat cggacatatc gacccaggag ctgcttctag tgccatttta
    10561 ttttcaacgc ttttagatgt gatcaatcaa ccttaagttc aataaaactg cggtgatgct
    10621 catcgcagtt ttttgtttgg ctgttcttat gacttgatgc agaacagctt tttcacaacc
    10681 tctatttgtc catatcgcaa aattcaggta gaatacaaaa gataacggaa agaggcgaaa
    10741 caatggaact tggaaaatca gattatcagt tgtttgatcg accaatctac gcattcaaac
    10801 agctcaaaga gagccatcca acagataaga ttgaacaaat caagaaagaa tataaagaac
    10861 actggcaaaa atggaaagag attcagctcc agacagctgc acttttgcct gatatgtacg
    10921 gcatgagcaa acctaaaatc gaaagctgga cgaatggctg gaatttacgc agtcattttt
    10981 ggagcgcgta ccgaagcgaa tctcgtcaag acgagaatgc ctgtctagca gttttgttga
    11041 atcaaaaaca ataccaaatc tatttgatgt accaacatta taaaagcgaa gaacgatatg
    11101 gtagcattgc agcgtataac cagttgcttg cgatactgaa agagtggagc aaaacagtcg
    11161 atatcaagga ttattacata tggccgcaac cagaacatga acttgacgac cacttagctt
    11221 tgtctgacta tttgtccgat acaaagaaac aagaagagtt gaaaaaagcg atgagagacc
    11281 gaacctttca aatgggtaaa ctattcttct atccgcaaga aatcgaacac gttgagcaaa
    11341 taaccgctga aaccttacaa gaactagcac cactatatca aaagctaggt gcggaaaaat
    11401 ttcaatagac aacacgaaat aagcaatgcg aaaaacaaac cttactctcg gcttgcgagt
    11461 gaaggtttgt ttttgattat tcaaaaagta aatatttaaa aaattgatag gaatcaatta
    11521 cttaaggtct caaaagaagg aaaataaagc aagggacaaa aagaaatcca ctctatttgc
    11581 tggtatctgg ttcgcttgac gtttatgcta tgataaaaga aaattatcag ttggaataag
    11641 ggaagttatg tttctttatt agaaatagct gtttagaagg aggagagatt atgagtcatg
    11701 atcatttatg tgtttcattt gttcctttgt tcaatcactt agtacagcaa gatcaagaaa
    11761 aagtacacgc acttgtcaaa catcactatg tggaaaaagg agaacagatt cttgcgccga
    11821 atggagaacc tcaattagtg atcgtggcct ccggcagtat gaaagtgtat caattatctg
    11881 ctagcggaaa ggaacagctt ttacgagtaa tagagccggg cggatatgag ggagagagcc
    11941 agttgctagg cgttccaaat gagtctttat ttggcgaagc actagaaaaa acggaaattt
    12001 gcatattgag acaaaaagat ttctcggaac tcttactgca ttatcccgcc cttagtctga
    12061 aacttttagc aatcaacgtt gagaaattaa caaaagtaca gcagcagaca acttttttga
    12121 tgatggaaaa agtagaagaa cgagtcgctg tctatctgct tgacttagct aaagcgacag
    12181 ggaaggatca ctttttcctg ccaatgaaaa tgaaagaact tgctgcattt atcggtacga
    12241 cccccgaaac tttatctaga aaatttaaat tattagagga gaatggttat gtaaaaagaa
    12301 agaatcgaga agtctggatc attgataaag agcagttaga agacttgtaa ataaaaaaat
    12361 gacctccaaa tgttggattt ttgacccaga tttggaggct agcatgaaat tgtacatgct
    12421 tttttattat ccttctagta agtctcttcg cctataacca atagaaccaa ggacgaacag
    12481 tgcaaggctg atacatgata acgtaaggaa cacggaaaga tcaaaagaat cgtttggcat
    12541 ccgtggaacc cagctaggga cagctgtttt ggaaaaccat tctggtaaat ccaaaattcc
    12601 gccaaagtaa ttgagtgcaa aggagtaagc taagtagata taagcaagtt taccgattcg
    12661 aggaagccat ccgaatagaa agccactaat tccgataaac actaaaacgg caggcaggaa
    12721 attgaacccg gctttaagaa acatcaatag atccatgtct ttcgcatcct tcatcgaagc
    12781 caaagcagaa agccctagtg caccagaact aatcaggatt gccaatatag caaatagaat
    12841 agctagtaac atattggtcc aataaagttg gttcctagaa atttttgttg cgaacagctg
    12901 attcatacgt ccgtgggttt cttctgtaaa taaacgattg atgctagcaa ttggtaaaat
    12961 agcagctaga caaatcaaga cgaccatgat cgtgcttgta aagttttttt caattgagac
    13021 gccagattgg ataaacatct gtttcattaa gtcgttactt tctaaaaact gttgcatatt
    13081 cccgtagata gagccgtatg ccgcccccat caaaccaaaa gtgataagcc aactgattgt
    13141 catcccttta tttagataga ataataaacc acgcaaggat aaaagagaac gtttagcata
    13201 cggttttcct tcacgttcgg ggatatagcc ggtgttcaaa tctcgatgtt cctctaaaac
    13261 aaatgaactg attatcaaga ttagactgaa caaaaacaaa taaagcaaag gaatccagtt
    13321 gttagttgta aatggatagg tcaaataaga ccagcctaaa ggattccaca tcgaaaggga
    13381 ggaatcagct acatctgtta ttgctcgtag aaggtaaagt ccacctaaaa acgctaaaga
    13441 cagaccattc gaactggctg aacttggcat cagttgtgca gcaaacagtg caacggcact
    13501 ccccaaaatc cccatactac ctacagatag cccaaaaagc caagcacctt ctgcagtgat
    13561 cgaatccgct tggaaaaaaa tcatcgtaca actgatcgat attgctaaaa gcaaattgat
    13621 cagaatactt tcagcgatga cagctaaaga gtttgcttga cgtccgactc gaaaggaacg
    13681 aataagttca atcagaccaa gttcttcttt ttttctcgtt tggctcacca cgtgtaaaac
    13741 agagatcaca gcagcaaaca aactgcaaaa aagcaacatt tcatttgcat acatcgctcc
    13801 taaagtatac tcagcagcat tcgaagctgg agttggtccg accattgcaa tcattgccgg
    13861 gttttgcaag gtttcgtata atccagctaa tccttgtccg gaagtcaatt cttcaaacgc
    13921 agggacaaaa ccaccagaaa aagcagtgac accgactagc catatcagga tctttttcca
    13981 gtcacggcta aggttttgag aaaaaagggt ggaccagcgt gaaaatttat cagtcatttc
    14041 gcttcctcct catccttcat aatgtctcat gaataaatcc tcaagagtcg gtggaataga
    14101 ttcaaatttt ttcacaccta atttggacgc ttcagctaaa atcacatcga aagcttcgtt
    14161 atcagcagca aattgagcga ggtgaccttt ttgcacaaag tcatggacac cgttcaattg
    14221 tgctaagtga ctattctctg cttctgtttc tatagtgatg gtcgaacgag tcaaatgacg
    14281 caattcatcg agtgttcctt cttcatcgag tgttccttcg cgtatgatca caactttatc
    14341 cgctagtcgc tcgacttcac tgagaatatg agaagaaagt aggatggatt tgccctgttc
    14401 tttcaatttt tcaacttctt cttggaaaat cgtttccatc aaaggatcta aaccagaagt
    14461 aggctcatca aataaataga gatccgaatc aacagccaaa gcagcaatca agcccacttt
    14521 ttggcgattt ccttttgaat agttttttgc ttttttctta ggatcaagag agaaacgttg
    14581 aatcagttca tctttttttt gagcactccc ttttccatga agcttcatga agagatcgat
    14641 gatcgctcct ccagtcaaat ttccccacaa agagatatcg cctggaacat aagaaagacg
    14701 ttcatggata gataaactat cttcccatac atcttgccca aaaatcgttg ccgttccgcc
    14761 gtcttttttt ataatcccta aaagtgtccg aatcgtcgtg gattttccag aaccatttgg
    14821 tccgataaat ccaacgactt cacctgaatg tacggtgaaa gagacatctt ttaaagcttc
    14881 gaaacgacca aagctctttt tcaaatgtgt gactttgacg atttctgcca taaaaacccc
    14941 actccttttt ctaaaaatat atactaaaca agattaaata gtttaataaa aaggttttat
    15001 acctttttat aa
//
LOCUS       DACEND010000065        14825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEND010000065 DACEND010000000
VERSION     DACEND010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.066"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..553)
                     /locus_tag="IW746_001663"
     CDS             complement(<1..553)
                     /locus_tag="IW746_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1078565.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGV"
     gene            complement(740..1390)
                     /locus_tag="IW746_001664"
     CDS             complement(740..1390)
                     /locus_tag="IW746_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078566.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1933..2868)
                     /gene="whiA"
                     /locus_tag="IW746_001665"
     CDS             complement(1933..2868)
                     /gene="whiA"
                     /locus_tag="IW746_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1078567.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2902..3900)
                     /locus_tag="IW746_001666"
     CDS             complement(2902..3900)
                     /locus_tag="IW746_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1078568.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3897..4781)
                     /gene="rapZ"
                     /locus_tag="IW746_001667"
     CDS             complement(3897..4781)
                     /gene="rapZ"
                     /locus_tag="IW746_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1078569.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4916..5647)
                     /locus_tag="IW746_001668"
     CDS             complement(4916..5647)
                     /locus_tag="IW746_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1078570.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5651..6916)
                     /locus_tag="IW746_001669"
     CDS             complement(5651..6916)
                     /locus_tag="IW746_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1078571.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7179..9995)
                     /gene="uvrA"
                     /locus_tag="IW746_001670"
     CDS             complement(7179..9995)
                     /gene="uvrA"
                     /locus_tag="IW746_001670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1078572.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10005..11999)
                     /gene="uvrB"
                     /locus_tag="IW746_001671"
     CDS             complement(10005..11999)
                     /gene="uvrB"
                     /locus_tag="IW746_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1078573.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12258..13760
                     /locus_tag="IW746_001672"
     CDS             12258..13760
                     /locus_tag="IW746_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880041.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1078574.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13762..14499
                     /locus_tag="IW746_001673"
     CDS             13762..14499
                     /locus_tag="IW746_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078575.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14544..14705)
                     /locus_tag="IW746_001674"
     CDS             complement(14544..14705)
                     /locus_tag="IW746_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1078576.1"
                     /translation="MIIIVSEGGIDYVLDIVNEPMVLSYAMEQVERLLVCAGEQAMRL
                     VQLSEKTRN"
BASE COUNT         4351 a         3184 c         2498 g         4792 t
ORIGIN      
        1 tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac aaccttgatc
       61 cttcttttat agattcagga aacagtataa ttttagtact agataaattg tttacaccga
      121 ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac aaaagaacag
      181 ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta taaatactct
      241 ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct atccaagcaa
      301 cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt aatctattta
      361 tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa ttaaaaatcg
      421 gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa taacctgatt
      481 ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat atccataatc
      541 tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag catcaccaat
      601 tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc atgcttcaac
      661 aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac ataaatttta
      721 cattctgttt tttataagac taataataat cattgaaaaa atttcttatc ggtggcgcat
      781 attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct tcttctaagt
      841 catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc tgtggcttgc
      901 ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt gttacatctt
      961 cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta ttatttaaaa
     1021 gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa cttcctataa
     1081 attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca tattcaagac
     1141 taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt ctccaaataa
     1201 atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg agacctgctc
     1261 gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct agaaaaataa
     1321 aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt tgccctccct
     1381 ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt caggtataac
     1441 gtataccaac catacatccc aattactatt tatgaactta tgattatcac tgaataaatc
     1501 actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc gactagctaa
     1561 tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac cagaagtaag
     1621 aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa tctccagtta
     1681 tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa gtttatgtat
     1741 aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat taaaaaacaa
     1801 tagaagcctt tcaacattat gttactccag taaagttgga attatagttt gacggaagaa
     1861 aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc atgtatcagt
     1921 tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat ttattttgcg
     1981 aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt cccctaattc
     2041 tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa gtttttcagg
     2101 aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt gtttagaagc
     2161 cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat tgaccgaatt
     2221 tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg ttgcaccaat
     2281 caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat agccattgcg
     2341 ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa tgtcttggtt
     2401 atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg gattattgac
     2461 tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg aatttcccat
     2521 aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa gatccatcaa
     2581 cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt ttaatttcat
     2641 ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa gagaatagat
     2701 acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct ggttcacaat
     2761 acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat gttctcgatg
     2821 aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc tattctcctc
     2881 ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga acaattcttc
     2941 tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa agtcggtgga
     3001 aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt gtactagata
     3061 ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat tgaccaatac
     3121 tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat cggcatctgt
     3181 gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg tttcagcagt
     3241 tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga tactggtgaa
     3301 taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa cgacttttcg
     3361 agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt caatggtttt
     3421 acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa aaacagcatg
     3481 caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca tcatttttgc
     3541 taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg caatgatcag
     3601 attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt attggaaaat
     3661 atcttcatag aattgtggca tatccgataa cgccactaag acatttctca agtcaccagg
     3721 aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat ctgcaacagt
     3781 cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa ctggcagacc
     3841 agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt tcattttcac
     3901 gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt gactttatag
     3961 tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg ttgaccgcct
     4021 gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc aggcaataca
     4081 gtttccagaa gatccagaaa tttatggtag aatgattgcg tttcatcaaa ggacatcaca
     4141 taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat ataatgaggg
     4201 ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc gtatttaaaa
     4261 ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa tgactcattg
     4321 attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac ggaagcttgg
     4381 gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat caaaccgtcc
     4441 atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc ttcgtctgaa
     4501 gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc gatcagcatg
     4561 ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc catttttgtg
     4621 actttacctg attctttgat caattcccag aattttggaa tcaatcgagg cggcatatta
     4681 tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt ccctgcacca
     4741 ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa cccctctcac
     4801 attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc tttttctttt
     4861 acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa tttttttact
     4921 tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg atcgttcgat
     4981 ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca aattcatgca
     5041 tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc tctaccgctt
     5101 cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg atctcatgat
     5161 agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt tcgaaaccaa
     5221 gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt cctagaatcg
     5281 ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt ggcatatgca
     5341 agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg gtgttacaag
     5401 tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc agcagaaacg
     5461 gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga actgttgcat
     5521 gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt cggtggttga
     5581 tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata ctgaaaaaat
     5641 tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga atatagttat
     5701 ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca taaaatacag
     5761 tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct tcattttccc
     5821 gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct ttatctgatt
     5881 gattcgttcc ataggtcgtt tcaacaaacg gtttgttgaa cactcgatct tctgtgacaa
     5941 aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct tgtctcaaat
     6001 tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg aaattggcaa
     6061 agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt ttttcgttat
     6121 aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct tctaatagtg
     6181 gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca tttagcacac
     6241 gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt ttataaccgg
     6301 cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa ataaaagctt
     6361 tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt ttcaaagcaa
     6421 ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt tcttctctga
     6481 cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa aagcttactt
     6541 tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa tattctgaca
     6601 gctcttgttt atgttgggag atcaattctg catatccatc accacaagca atcaaaagat
     6661 attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc tcgataaata
     6721 cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg tagcgggtag
     6781 gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca ttaaaagaac
     6841 gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat tcttcatttt
     6901 caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct tttccaacta
     6961 cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaactcctc cgattgctca
     7021 attctttttt gaacggattg aacttatagg aagtttttat ctataccaat cagtgtagaa
     7081 agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa aaattcattt
     7141 ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat ttttttcaaa
     7201 tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc agtcgcaagg
     7261 atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc tgctgttttg
     7321 attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac taatctttgt
     7381 aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg ttcatctagt
     7441 atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt catccgttgt
     7501 gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc taagccaaca
     7561 tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa ttctacagca
     7621 tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg cacttctaat
     7681 gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac atcaggcaaa
     7741 aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg accgcctttt
     7801 acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt cgtttgagca
     7861 aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact tctcggcgtc
     7921 cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta gccggaaatt
     7981 gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa cgatttcttt
     8041 aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac tgctacaaat
     8101 tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc accagtgatc
     8161 ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga ttttttccct
     8221 gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg agtgcctgca
     8281 gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa ataatctgct
     8341 gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag atcacgcatt
     8401 ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat cgatggttca
     8461 tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa tctgattcgc
     8521 tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata gtctagtccc
     8581 acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt ggcaattact
     8641 ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg aatagacaac
     8701 tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg gggattcaat
     8761 cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat ttgctcacgc
     8821 gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac cccttcgaaa
     8881 gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg gaaaagatca
     8941 tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa tggtgtatct
     9001 aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata ctgagagctg
     9061 attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc tggaatcact
     9121 aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca agctccaaaa
     9181 ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc gcaataagga
     9241 caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac cagagcatag
     9301 ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg aatcccttct
     9361 ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt ctccagctca
     9421 ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta tccctctttt
     9481 tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat cggtgccaac
     9541 acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg atctactgat
     9601 tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg tgcaaacaac
     9661 aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg atttttactt
     9721 gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc tacgtccggc
     9781 ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac gtatctccgc
     9841 tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc ggacaaacct
     9901 gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa gttatgggca
     9961 cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt tgaagctttt
    10021 aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc ttttgccgca
    10081 tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt cgtcatttct
    10141 tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt aatagaaatc
    10201 aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc attgtacttc
    10261 tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga atctgttact
    10321 ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc aatcgtttgg
    10381 actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat agcaacaagg
    10441 gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa cacatcaaat
    10501 tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat atcactatgc
    10561 aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc tgccattttt
    10621 ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt tatttcgcct
    10681 actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc aagcagccct
    10741 gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata tggtcctggc
    10801 gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa ccgaagtggc
    10861 cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg ttttctcgct
    10921 cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc gtccgccacg
    10981 atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc ttctttacgt
    11041 ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc taacatcatt
    11101 tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt gttttcattt
    11161 cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc atgctccata
    11221 tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ccacgtgttc cgtttctcct
    11281 aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc tccaaaaaac
    11341 tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac atcaccgcgc
    11401 actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat actgactaag
    11461 tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac tacttgtttt
    11521 tggtactcaa aaggcgaacc taatccaaaa atacatgaaa cggatgcaat gacgatcaca
    11581 tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat ctcatcattt
    11641 acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc aggctgataa
    11701 taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc cttgaattcc
    11761 ccgtacagct gaccagccaa tgttttatta tgagcaataa tcagtgttgg tttattcact
    11821 ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc taataagatt
    11881 tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc aggctgatca
    11941 ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct ctcaatcatt
    12001 cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag aaaaggaaag
    12061 caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc acttttttat
    12121 cttattatac atgttacgtt ttataacata aaatgttaga aactcgcatt tttttcattt
    12181 tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata aaatattgga
    12241 aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt ttcttaatga
    12301 caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact acaacggcag
    12361 agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta tacgtaattg
    12421 ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag tacgaaggca
    12481 tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc cgaatggact
    12541 accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac ggaatgattg
    12601 ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat tcttactttg
    12661 atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct tatgaagatt
    12721 tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt ttaagcgacc
    12781 atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa ttgtacgacg
    12841 ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc ggttatgcga
    12901 tcaaacaagg acaagaattg gaaacaccga tcgaaaaaga atctggcgga aaatatgcat
    12961 ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa ggattgaaag
    13021 aattaaaaag aacaggcgaa tacgataaga ttatcaaaga atatgtagcg gatggttcag
    13081 aaacttctag tgagtcttcc actgctgatg aatcaacatt tcttggactt atccaaaaca
    13141 actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg atctcctttg
    13201 ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca atacgcgctc
    13261 ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg atggttcttg
    13321 cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct gacttccttg
    13381 ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt gttcgaggcg
    13441 ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt ctttcttata
    13501 atcggacaat gcaaaaaatc attttgccac aagcaatacg catcatgatc ccatcattcg
    13561 tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc ggtgtagtcg
    13621 aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg tatgtttact
    13681 tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta gcaaaagttt
    13741 tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt agtcaaaaaa
    13801 tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg agatgtcgtc
    13861 tgtatcattg gcccttcagg ttcaggaaaa agtacatttt tgcgttgctt gaatcgttta
    13921 gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga taaaagcaca
    13981 gatatcaact tggtgagaca acatatcgga atggtattcc agcactttaa cctattccct
    14041 catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa agaatctaga
    14101 gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga agataaaaaa
    14161 gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat tgctcgtgct
    14221 ctagcgatga accctgacat catgcttttt gatgagccta catctgcact cgaccccgaa
    14281 atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat gacgatggtt
    14341 atcgttacgc acgaaatggg cttcgctaaa gaagtggcca atcgtgtcat gttcattgac
    14401 gacggaaact ttttagaaga cggaacgcct caacaaatct ttgaaaatcc gcaaaatgaa
    14461 cggacaaaag atttcttgaa taaagtattg aatatttaat aaaaaccaaa aatgcccggt
    14521 tgcttcatga cgaccgggca tttttagttt cttgtttttt cacttaactg taccagccgc
    14581 atcgcttgtt ctcctgcaca aacaagcaat cgctctacct gttccatcgc ataagaaagt
    14641 accatcggtt cattgacgat atcaagtaca tagtcgatac caccttcaga aactatgatg
    14701 attatcttat tctaaagctg gcttttttgt tggtacaaac atgacaacta caaaaaaacc
    14761 aggcgaacgt atgattgttc gtctggtttt taattattaa tgacttagcg tttttactag
    14821 aaata
//
LOCUS       DACEND010000066        14398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEND010000066 DACEND010000000
VERSION     DACEND010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.068"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1421)
                     /gene="nhaC"
                     /locus_tag="IW746_001675"
     CDS             complement(3..1421)
                     /gene="nhaC"
                     /locus_tag="IW746_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1078577.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1824..3047)
                     /locus_tag="IW746_001676"
     CDS             complement(1824..3047)
                     /locus_tag="IW746_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1078578.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3078..3917)
                     /locus_tag="IW746_001677"
     CDS             complement(3078..3917)
                     /locus_tag="IW746_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1078579.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3934..4614)
                     /locus_tag="IW746_001678"
     CDS             complement(3934..4614)
                     /locus_tag="IW746_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078580.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4616..5689)
                     /locus_tag="IW746_001679"
     CDS             complement(4616..5689)
                     /locus_tag="IW746_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078581.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6336..7007)
                     /locus_tag="IW746_001680"
     CDS             complement(6336..7007)
                     /locus_tag="IW746_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1078582.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7094..9772)
                     /gene="mgtA"
                     /locus_tag="IW746_001681"
     CDS             complement(7094..9772)
                     /gene="mgtA"
                     /locus_tag="IW746_001681"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1078583.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10431..10868
                     /gene="ndk"
                     /locus_tag="IW746_001682"
     CDS             10431..10868
                     /gene="ndk"
                     /locus_tag="IW746_001682"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1078584.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10890..11756)
                     /locus_tag="IW746_001683"
     CDS             complement(10890..11756)
                     /locus_tag="IW746_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1078585.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11916..14009)
                     /locus_tag="IW746_001684"
     CDS             complement(11916..14009)
                     /locus_tag="IW746_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1078586.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4179 a         3086 c         2446 g         4687 t
ORIGIN      
        1 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
       61 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
      121 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
      181 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
      241 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
      301 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
      361 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
      421 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
      481 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
      541 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
      601 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
      661 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
      721 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
      781 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
      841 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
      901 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
      961 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
     1021 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
     1081 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
     1141 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
     1201 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
     1261 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
     1321 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
     1381 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
     1441 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
     1501 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
     1561 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
     1621 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
     1681 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
     1741 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
     1801 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
     1861 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
     1921 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
     1981 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
     2041 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
     2101 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
     2161 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
     2221 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
     2281 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
     2341 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
     2401 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
     2461 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
     2521 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
     2581 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
     2641 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
     2701 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
     2761 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
     2821 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
     2881 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
     2941 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
     3001 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
     3061 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
     3121 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
     3181 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
     3241 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
     3301 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
     3361 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
     3421 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
     3481 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
     3541 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
     3601 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
     3661 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
     3721 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
     3781 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
     3841 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
     3901 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
     3961 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
     4021 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
     4081 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
     4141 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
     4201 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
     4261 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
     4321 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
     4381 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
     4441 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
     4501 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
     4561 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
     4621 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
     4681 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
     4741 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
     4801 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
     4861 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
     4921 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
     4981 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
     5041 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
     5101 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
     5161 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
     5221 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
     5281 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
     5341 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
     5401 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
     5461 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
     5521 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
     5581 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
     5641 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
     5701 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
     5761 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
     5821 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
     5881 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
     5941 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
     6001 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
     6061 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
     6121 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
     6181 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
     6241 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
     6301 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
     6361 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
     6421 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
     6481 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
     6541 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
     6601 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
     6661 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
     6721 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
     6781 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
     6841 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
     6901 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
     6961 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
     7021 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
     7081 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
     7141 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
     7201 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
     7261 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
     7321 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
     7381 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
     7441 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
     7501 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
     7561 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
     7621 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
     7681 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
     7741 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
     7801 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
     7861 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
     7921 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
     7981 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
     8041 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
     8101 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
     8161 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
     8221 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
     8281 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
     8341 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
     8401 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
     8461 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
     8521 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
     8581 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
     8641 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
     8701 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
     8761 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
     8821 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
     8881 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
     8941 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
     9001 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
     9061 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
     9121 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
     9181 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
     9241 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
     9301 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
     9361 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
     9421 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
     9481 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
     9541 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
     9601 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
     9661 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
     9721 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
     9781 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
     9841 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
     9901 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
     9961 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    10021 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    10081 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    10141 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    10201 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    10261 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    10321 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    10381 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    10441 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    10501 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    10561 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    10621 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    10681 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    10741 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    10801 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    10861 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    10921 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    10981 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    11041 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    11101 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    11161 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    11221 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    11281 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    11341 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    11401 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    11461 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    11521 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    11581 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    11641 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    11701 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    11761 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    11821 atacacgaaa aaaataaaaa aatgcttttt tcataaaaaa taccaacctt caagcattag
    11881 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    11941 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    12001 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    12061 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    12121 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    12181 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    12241 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    12301 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    12361 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    12421 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    12481 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    12541 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    12601 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    12661 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    12721 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    12781 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    12841 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    12901 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    12961 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    13021 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    13081 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    13141 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    13201 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    13261 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    13321 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    13381 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    13441 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    13501 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    13561 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    13621 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    13681 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    13741 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    13801 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    13861 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    13921 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    13981 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    14041 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    14101 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    14161 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    14221 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    14281 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    14341 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattg
//
LOCUS       DACEND010000067        13956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEND010000067 DACEND010000000
VERSION     DACEND010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.069"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            239..481
                     /locus_tag="IW746_001685"
     CDS             239..481
                     /locus_tag="IW746_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1078587.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            478..948
                     /locus_tag="IW746_001686"
     CDS             478..948
                     /locus_tag="IW746_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1078588.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1076..2290
                     /locus_tag="IW746_001687"
     CDS             1076..2290
                     /locus_tag="IW746_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1078589.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(3000..3188)
                     /locus_tag="IW746_001688"
     CDS             complement(3000..3188)
                     /locus_tag="IW746_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078590.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3279..3488
                     /locus_tag="IW746_001689"
     CDS             3279..3488
                     /locus_tag="IW746_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1078591.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3600..5213
                     /locus_tag="IW746_001690"
     CDS             3600..5213
                     /locus_tag="IW746_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1078592.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5248..6459
                     /locus_tag="IW746_001691"
                     /pseudo
     CDS             5248..6459
                     /locus_tag="IW746_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6482..7408
                     /gene="glsA"
                     /locus_tag="IW746_001692"
     CDS             6482..7408
                     /gene="glsA"
                     /locus_tag="IW746_001692"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1078593.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7616..9076
                     /locus_tag="IW746_001693"
     CDS             7616..9076
                     /locus_tag="IW746_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078594.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9297..11060
                     /locus_tag="IW746_001694"
     CDS             9297..11060
                     /locus_tag="IW746_001694"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1078595.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11144..11602
                     /locus_tag="IW746_001695"
     CDS             11144..11602
                     /locus_tag="IW746_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1078596.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11711..12394
                     /locus_tag="IW746_001696"
     CDS             11711..12394
                     /locus_tag="IW746_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1078597.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12541..13155
                     /locus_tag="IW746_001697"
     CDS             12541..13155
                     /locus_tag="IW746_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1078598.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13171..13377
                     /locus_tag="IW746_001698"
     CDS             13171..13377
                     /locus_tag="IW746_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078599.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13888..13956
                     /locus_tag="IW746_001699"
                     /pseudo
     CDS             13888..13956
                     /locus_tag="IW746_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
BASE COUNT         4840 a         2200 c         2907 g         4009 t
ORIGIN      
        1 aaaataagaa attgattgtc gttttctttt gcagaaattt aagtgcaggc aattatggta
       61 gttgtcgtaa ttgctgtcag ctcagtgttt atcactgatg gtgtgaaaga tttacggttg
      121 gagatgttca ataagattat tggtgcttaa ttatttttat tgtagtgctg atatgagaat
      181 gttataattg gggtataaaa catacaaata aatttgctga aaaatgaggt atcaaaatat
      241 ggaaaatgaa ttactggaaa acttaggaag acttcacaca actgaacttg gcgttattag
      301 aattaaaaag aacttatctt tgaatgtaga aaatgtaatt gaatggtgca aagaaaaaat
      361 tagcctttct aatgcggaaa tcacaagaaa aggaaaaaac tggtatataa cgattgataa
      421 ttgtattata acggtaaatg cgtacagtta taccgttatc actgctcaca aggtaaaatg
      481 ataaattttt ctataaagga attaaacgca tttgcagtta ttgggcagga agtagaactg
      541 actaattatc aaaaaaagaa tattcagatt agtacacagt tttggagaaa gtttaacagt
      601 agtttgaaaa aagcgtatct ttcacaatca ggaaattggg taaaatatgc ttttatggaa
      661 agaagaaatg gaaaacttta ttatttctgt tctattcccc aaagaaccat taccccagag
      721 ggctttctgt ataaagaaat accgtcttat aaatatttgg ttgtggagca tattggtgct
      781 atggataaaa tatatgaaac ttatggcaag atttatgaag aaattattcc tagtacatcg
      841 tatattccta taaaagatat tatcctacac tttgaaaaat acgattatcg ttttcattgg
      901 aatagtgata attcagttat tgaaatttgg atacctattc aagattagaa gcaattttat
      961 atccatacac acaaagcagt tagtcttagg attgactgct ttttctatac agatttttaa
     1021 tgacaggcaa ttatcaatta tggtagttgc cttttttgat tggaggaatt gaagaatgaa
     1081 tgataaaaac tttattgaag aactaagaca aaagcgtgag gaatacggag taacacaaac
     1141 aaggcttgct gttgcctgtg gtatcagccg tgaatattac aaccgcattg aaaaaggaaa
     1201 acagccattg aatgacgaat taagagaaat catagaaaaa cagattgagc gtttcaatcc
     1261 gcaagaacca ctattcttat tgattgatta ctttcgtgtc cgctttccta ctacggacgc
     1321 attggcgatt atccgtgatg tattacaact gaaatctaat tacatgcttt atgaagatta
     1381 tggaaaatac ggatatgaaa gcaaatatgt acttggcgac atcaatatca tgtgttccat
     1441 gcaggagcat ttaggtgttt tattggaact gaaaggcaaa ggctgtcggc aaatggaatg
     1501 ttatctgctg gcacaggaac gctcatggta tgacttcatg ctggattgta tgacggctgg
     1561 tggtgtgatg aaacggcttg atttggctat caatgataaa gcaggaatat tggatattcc
     1621 aaagttaaag gaaaaataca aggctggcga atgtatgtcc tacttccgta tgcagaaaga
     1681 ttacagcggt acagaaaaat gcggtagcga tttaccaaag aatacaggag aaaccttata
     1741 tctcggttca acaagtagcg aattatatat gtgtgcttat cagaaaaatt atgagcagta
     1801 tgtcaagaat ggcacagaaa ttgaagatac ggagattaag aaccgttttg aaatacgcat
     1861 gaagaacgaa cgagcctatt atgcggttgt agatttactg acctatcggg acgctgaacg
     1921 caccgctttt tctatcatca atcattatgt ccgctttgtt gacagagagg acgacaagcc
     1981 aaaaagtcaa tggataacaa atgatgattg ggcatggttt gtaggggaaa acagagagcc
     2041 gatacgctta accacaaagc cagagcctta cactttacaa aaagcgttgc attggctaca
     2101 aagacaagtc gcaccaacca taaaaatggt acaggcatta gacagagaaa atcgtacaac
     2161 gatactgaaa gatatgattg agcaggcaga actaaaagac aagcataaac atttattaca
     2221 attagagaaa tcaaccatag aagaacgtat agataccgct gttccacaag agaatgacgg
     2281 tattttttaa tgtaattgaa aaatttcaga tttagtcagc aaagagctgt tttcattccc
     2341 tatgtgaagt gaaagaagaa aaataaattt tttattaaat ctgcaacaaa atggctactt
     2401 gcgtacagat atggtgcgag aaaaggaaat ctaaactttt tttgaaaata ggtcattaaa
     2461 tcggggcttg ctgtccctat atgaagtgaa agaagaaaaa agttttgaaa aaatgacgga
     2521 aattcgtgat tagtggacag tcttctgtga aaagaggaaa aacatcatca ttttgtattc
     2581 aaacaggcat tagcagttga gtgttgatgt gaaaagatga aaatactttg ttctcaactt
     2641 aacaatttaa ggtttccgtt ccctatatgg tgcaaagaaa atatttcatt ctattgttgg
     2701 cagtaacggt atggtttggg tagttagggt gtgaaaagga aaatattctt ttttcatcat
     2761 caacttagca aaatgacatt ttctatccct atatggtgca tgaaaagaag ttaaaatttt
     2821 tccatttaaa cttagcaaat ggacgtttgc tgtacagata gtagtgaaaa aggagaaaat
     2881 caaccgttga ttgccaaaat gaaccgttcc atttgtacta ataatggaaa aagttttgga
     2941 ttttggttac gtttctcctc ttttccaacg cgatatatag gaaagacgat ttttttcttt
     3001 caatcgttct ttgacaactg aataaagcta ttcaatacgt ttgacagaca gctagccgtt
     3061 gaaacaaata cgccacgacc tttcccctgc aagagcgagc gaaaacctat ttgtgttctt
     3121 gtaaaagatt tgtcctcttt tatcgccatg acctgtactg tcagataatg atactcccgt
     3181 acagccacag cttgagcatt aagagcgtcg cacgcaatgg gtacggctac ataagaacta
     3241 tgcagaggtg gaactcctgt gggctatgac aaaagccgtt gaattgctta gaaagatttt
     3301 acagaaaggg ggtatgtgtt attgataatg ctgtaaaaac aattcaaaag aaaaatggca
     3361 agtgtggcat tggcaacaga ggaaagacaa agattgtagt aaaagagcat ttttccgaaa
     3421 agggcaaaac aatggaagaa cttctaactg atgtcatgct ggaaaaagca aagcagacaa
     3481 tcgcataaaa tcggtgcagt tgtaagaaat agattgaatg acaaaaagta cccgtgttat
     3541 actttacttg caagcaggta ttgtaaacac gggttagtta taaaggagga taactgacaa
     3601 tgaaacaaca gatttacaat actgcacttt atcttaggtt aagccgagat gatgaattac
     3661 agggcgaaag ttccagcatt accacacaga gaagtatgtt gcgtttatat gcaaaagaac
     3721 atcatttgaa tgtcattgat gaatatattg atgacggctg gtcgggaaca aattttgaca
     3781 gaccgagttt tcaaagaatg attgaggata tagaggcagg aaaaatcaac tgtgttgtaa
     3841 cgaaagacct ttcccgtctt ggcagaaatt atattatgac aggacaatat acagaattgt
     3901 atttccctag tcataatgtc cgctacatag cgattgatga cggtgtagac agcgaaaaag
     3961 gcgaaagtga gattgcacca tttaagaaca tcatcaatga atgggtggca agagatacaa
     4021 gccgtaaagt caaatcagca tttaagacaa agtttgcgga gggggctcat tacggtgctt
     4081 atgctccgtt aggatataag aaacaccctg acatcaaagg aaaactgttg gttgatgatg
     4141 aaacaaaatg gattattgaa aaaatctttt ccctagccta tcaaggttac ggaagtgcaa
     4201 aaatcacaaa gcagttaaga gcagaaaaag ttccgacagc gtcatggcta aattttacaa
     4261 gatacggtac ttttgcacat atctttgagg gaaaacccga aagtaagcgt tatgagtgga
     4321 cgattgctca tgtcaaggcg atattgaaaa gtgaggttta tatcggaaac agtgttcaca
     4381 atatgcagtc tacggtatcg ttcaagagca aaaagaaagt gcgtaagccc gaaagtgaat
     4441 ggtttcgagt agaaaacact cacgagccga ttattgacaa ggaagtgttc tatcgtgtgc
     4501 aggagcagat aaaatcaaga cgcagacaga caaaggaaaa ggcaacgccg atttttgcag
     4561 ggcttgtcaa gtgtgcggat tgtggttggt ctatgagatt tggaacgaat aagacaaata
     4621 aaacgcctta cagttattat gcttgcagtt actacgggca gtttggcaaa ggtaattgtt
     4681 ctatgcacta cattcgttat gatgtgctgt atcaagccgt attggaacgc ttgcagtatt
     4741 gggctaaggc agtacagcag gacgaagaaa aggtattgaa caagatacag aaagctggca
     4801 atgcagagag aatacgggaa aagaagaaaa aggcaagtac attgaagaaa gccgagaaca
     4861 gacaaaatga gattgaccgt ttatttgcga aaatgtatga agatagagcc tgtgagaaga
     4921 taacagagcg aaattttgtg atgttgtcca gcaagtacca aaaagaacag atagaactgg
     4981 aacagcagat aacaagccta agagaagaac taagtaaaat ggaacaggat atgataggtg
     5041 ctgaaaagtg gattgagtta atcaaggaat attccgtacc aaaggaactg acagcaccat
     5101 tattaaatgc catgatagaa aaaatcctca tacatgaagc aacaacgaat gaggataacg
     5161 aaagaataca ggaaattgag atatattacc gatttattgg aagagttgag taatcaacaa
     5221 gagtaatatt tttaactaag ggaaaccggc ggtgtctata cttgggtcag tcgcacattg
     5281 ggaaaacgat tcggttttgc cgcaatcttt ttccaatggt ttgagataac agttggctac
     5341 ttgaccatgc tgtatttctt aactggtgca ttatcctatg caacgggtat tggtgctata
     5401 caaaacaata aatatctgaa attggcaatc ctattgatta ttttctgggc tattttactc
     5461 tcacagttaa aaggaaccaa atacacttca ttgattgctc ggatcggttt tgtagctggt
     5521 atcttgttgc cagcattagt cttgtttggc ttagggatcc attatgtcgc ttcaggcgct
     5581 ccactacaaa cgatactgtc tatgaaaaca ttgattcctg atttttcgaa attgcctacg
     5641 ctggttgttt ttgtttcctt tattttagca tatatgggcg tagaaacatc tgccagtcat
     5701 gcaaatgaaa tggaaaaccc taaaaaggat tatccattag caatgtttgt attggttatt
     5761 ctggcaatca ttttagatac ttttggcgga ttgaccgtcg ctactacagt acctcaacat
     5821 ggtttgtctc ttaatacagg ggtgattcaa tcgttagaat tcctattaag acacattgat
     5881 ccaaatcttg catggattgc gaaaattctt gcgattcttg tttgtttagg tgtcatcgga
     5941 gaaattgctt cttgggtaac ctctccttca aaagcacttc acgtagcagc agaagaagga
     6001 ttacttcctg aatattttgc aaaagaaaac acacacggtg ttcctgctca tttgatgatc
     6061 gcaaatggga tcgttgcaac aatctgggca gccgtcttta cattaagcgg cggggggaat
     6121 aatctttcat ttcttgcagc gatgtcattg actgttgtaa tctacctgat gttgtacttt
     6181 atgtttttcg cgggctattt gaagctggtt tttaaagatg acagtcttga aagagcctat
     6241 caggttccag gcggaaaagt tgtaaaagta atcgttgctt gtttaggtct tttgacttca
     6301 ctttttgctt tttgtatctc atttgttcca cctgctagta tcgcagctgg acagcatggg
     6361 atttatgaaa cgattctgat tgtttcttat cttatcgtct tagttattcc atttacgatc
     6421 tacgcatgcc gtcataaatt ccagcatgca gaaaaataaa aaatgaaagc tggttattca
     6481 aatggaagta aaagaattag aacaattgat tggaaaaaat ttccactatt atcaaacagg
     6541 acaagtggcg gattacattc cagcattagc tgaagtggat cccaaacaat taggtatggc
     6601 gatttatgat ttggatacaa aacaactgat tgagggagga gacgccgatg tgcgctttgc
     6661 catcgaaagc atgtcaaaag ttcccgtatt gttattagca atcaaagaca acggaataga
     6721 aaaagtcttt caaacaatca acacggaacc aaccggtttt gccttcaatt ctatcatgaa
     6781 tatggaaatc aatcatcgca aacatccaat gaatcctttt gttaatgcag gagcgattgc
     6841 aacaacctcg ttgattgccg gtaaagatgc agatgaaaaa tttgagcgaa tcttgtcatt
     6901 tatgaaagag atctgtgatg atcctaatat cacattgaat gaagaaatct atcattctga
     6961 atcaagaaca ggtgatatca atcgctctct tgcttactat atgaaaggta atcaaatgat
     7021 cgaaggagac gtaccggaga tactcgatgc ttatttcaga caatgctccc tcaatgtcac
     7081 cgctacaggc atagcaaaat tagctgctgt gctggcaaat aaaggaattg ctccttggaa
     7141 tggcaaacaa atcattactg aagaaagtgc tacaatcgtc aaatcaatta tgacaactgc
     7201 tggtttgtat gatgaatcag gagagttttc cgtccatgtt ggtgtacctg caaaaagcgg
     7261 tgtaggcggc gggttgatgg cagctgtgcc aaatcgttac ggcataggtg tcttcagccc
     7321 ggcactagat ccatttggta acagcgcagc tggtatccaa ttgttgaaag atgtagtcaa
     7381 agaactagac gctgacatct ttgaataacc attcttgaaa gaggctgggg taaaagtcca
     7441 aacggctcaa agctacctac aatgaggctg aaattgtgag cttgtttgac ttttgtccca
     7501 gtttcttttt ttattggttt ttgaaagggc ttttctaaat aaaaaggaaa caaaaatacg
     7561 atttacagat atgattaaat aaaatcataa gaaaaactgg aggcgtgtat caggaatgct
     7621 ggaaaattac atcgagcgaa atatatttag aaaagtgtat ctgtgtgaac aattatttga
     7681 attccaagaa atagacatcg aacaaacagc tatttctcta agggtgacta caccaacgat
     7741 ccttcacgat ctagaaagtc tagccgagtg tctagaatat tgtataaaag aacaagtaag
     7801 agagaagcat aagtataaac tcgtattcaa acatggtatt gctctttctg agctcacaca
     7861 gtttctgtac ggtcaatctt attttttaaa gtttttaagt tattatttta atggtatatt
     7921 cacgagtaca gaactggctg atcttgaatt tatttctttg agtaaagtat atacaatcaa
     7981 aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat
     8041 cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg
     8101 ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga
     8161 gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgagaaat
     8221 agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag
     8281 cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat
     8341 tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta
     8401 taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt
     8461 tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga
     8521 cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc
     8581 agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct
     8641 gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag
     8701 atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca
     8761 agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt
     8821 ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt
     8881 atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt
     8941 gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat
     9001 ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa
     9061 aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc
     9121 tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt
     9181 ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata
     9241 aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga
     9301 gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg
     9361 ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg
     9421 atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt
     9481 atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc
     9541 ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg
     9601 gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat
     9661 ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg
     9721 tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca
     9781 atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg
     9841 atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa
     9901 gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg
     9961 atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac
    10021 ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat
    10081 atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag
    10141 aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc
    10201 ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa
    10261 aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt
    10321 ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac
    10381 gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg attattttag
    10441 tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt
    10501 acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct
    10561 ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac
    10621 atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag
    10681 taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag
    10741 aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga
    10801 tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca
    10861 cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg
    10921 attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg
    10981 tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc
    11041 gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta
    11101 tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga
    11161 agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt
    11221 cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa
    11281 gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc
    11341 aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa
    11401 aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga
    11461 aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca
    11521 tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga
    11581 atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc
    11641 tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata acattactgg
    11701 aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc
    11761 tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa
    11821 agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg
    11881 aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt
    11941 tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc
    12001 actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat
    12061 gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca
    12121 agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc
    12181 acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat
    12241 cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt
    12301 aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag
    12361 atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga
    12421 caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta
    12481 cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc
    12541 atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt
    12601 tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat
    12661 atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg
    12721 atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa
    12781 gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat
    12841 ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc
    12901 atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat
    12961 gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa
    13021 tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg
    13081 aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt
    13141 gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca
    13201 gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac
    13261 ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag
    13321 gaaatagaaa tgccagatca aacgattagc ggattgttta attactttaa caaataaata
    13381 tttattccga aaagtgaaaa aacatataaa aaaacacata aaactaaaaa attatcaaag
    13441 aatcctggtg caactgaaag aatttgcaac gggattcttt caacttattt gttatttttt
    13501 tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc attgcctaat
    13561 cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct tgttaggtga
    13621 ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac
    13681 agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag
    13741 tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg
    13801 accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg
    13861 acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg gtggattttg
    13921 ggtccttatt tgactttgtc catgatcggt ctttta
//
LOCUS       DACEND010000068        13891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEND010000068 DACEND010000000
VERSION     DACEND010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.070"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(183..1469)
                     /locus_tag="IW746_001700"
     CDS             complement(183..1469)
                     /locus_tag="IW746_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1078600.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1462..2730)
                     /locus_tag="IW746_001701"
     CDS             complement(1462..2730)
                     /locus_tag="IW746_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1078601.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2982..3443
                     /gene="mscL"
                     /locus_tag="IW746_001702"
     CDS             2982..3443
                     /gene="mscL"
                     /locus_tag="IW746_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1078602.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3502..3948)
                     /locus_tag="IW746_001703"
     CDS             complement(3502..3948)
                     /locus_tag="IW746_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1078603.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4041..4286)
                     /locus_tag="IW746_001704"
     CDS             complement(4041..4286)
                     /locus_tag="IW746_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078604.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4400..4978)
                     /locus_tag="IW746_001705"
     CDS             complement(4400..4978)
                     /locus_tag="IW746_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1078605.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5055..5585)
                     /locus_tag="IW746_001706"
     CDS             complement(5055..5585)
                     /locus_tag="IW746_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1078606.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5589..6449)
                     /gene="rlmB"
                     /locus_tag="IW746_001707"
     CDS             complement(5589..6449)
                     /gene="rlmB"
                     /locus_tag="IW746_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1078607.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6442..6837)
                     /locus_tag="IW746_001708"
     CDS             complement(6442..6837)
                     /locus_tag="IW746_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1078608.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6834..8246)
                     /locus_tag="IW746_001709"
     CDS             complement(6834..8246)
                     /locus_tag="IW746_001709"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1078609.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8243..8779)
                     /gene="cysE"
                     /locus_tag="IW746_001710"
     CDS             complement(8243..8779)
                     /gene="cysE"
                     /locus_tag="IW746_001710"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1078610.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9101..10561)
                     /locus_tag="IW746_001711"
     CDS             complement(9101..10561)
                     /locus_tag="IW746_001711"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1078611.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10731..11888)
                     /locus_tag="IW746_001712"
     CDS             complement(10731..11888)
                     /locus_tag="IW746_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1078612.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11922..13292)
                     /gene="radA"
                     /locus_tag="IW746_001713"
     CDS             complement(11922..13292)
                     /gene="radA"
                     /locus_tag="IW746_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1078613.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13355..13882)
                     /locus_tag="IW746_001714"
     CDS             complement(13355..13882)
                     /locus_tag="IW746_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1078614.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3643 a         3033 c         2491 g         4724 t
ORIGIN      
        1 cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat
       61 ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt
      121 ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg
      181 gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca
      241 gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt
      301 cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat
      361 tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc
      421 ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg
      481 tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca
      541 aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct
      601 aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct
      661 tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct
      721 tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct
      781 tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca
      841 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
      901 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
      961 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
     1021 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
     1081 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
     1141 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
     1201 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
     1261 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
     1321 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
     1381 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
     1441 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
     1501 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
     1561 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
     1621 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
     1681 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
     1741 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
     1801 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
     1861 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
     1921 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
     1981 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
     2041 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
     2101 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
     2161 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
     2221 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
     2281 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
     2341 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
     2401 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
     2461 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
     2521 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact
     2581 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
     2641 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
     2701 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
     2761 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
     2821 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
     2881 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
     2941 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
     3001 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
     3061 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
     3121 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
     3181 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
     3241 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
     3301 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
     3361 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
     3421 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
     3481 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
     3541 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
     3601 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
     3661 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
     3721 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
     3781 cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc
     3841 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
     3901 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
     3961 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
     4021 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
     4081 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
     4141 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
     4201 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
     4261 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
     4321 ttttaaaatt ttcaagttta ccataaaaaa aaagaaatgc aataaaaata aataagaagc
     4381 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
     4441 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
     4501 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
     4561 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt
     4621 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
     4681 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
     4741 tttgattgct tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt
     4801 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
     4861 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
     4921 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
     4981 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
     5041 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
     5101 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
     5161 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
     5221 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
     5281 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
     5341 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
     5401 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
     5461 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
     5521 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
     5581 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
     5641 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
     5701 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
     5761 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
     5821 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
     5881 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
     5941 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
     6001 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
     6061 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
     6121 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
     6181 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
     6241 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
     6301 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
     6361 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
     6421 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
     6481 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
     6541 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
     6601 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
     6661 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
     6721 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
     6781 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
     6841 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
     6901 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
     6961 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
     7021 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
     7081 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
     7141 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
     7201 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
     7261 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
     7321 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
     7381 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
     7441 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
     7501 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
     7561 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
     7621 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
     7681 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
     7741 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
     7801 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
     7861 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
     7921 gtagcaggct cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
     7981 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
     8041 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
     8101 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
     8161 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
     8221 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
     8281 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
     8341 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
     8401 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
     8461 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
     8521 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
     8581 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
     8641 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
     8701 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
     8761 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
     8821 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
     8881 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
     8941 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
     9001 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
     9061 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
     9121 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
     9181 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
     9241 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
     9301 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
     9361 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
     9421 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
     9481 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
     9541 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
     9601 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
     9661 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
     9721 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
     9781 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
     9841 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
     9901 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
     9961 caacagcaaa attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga
    10021 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    10081 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    10141 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    10201 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    10261 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    10321 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    10381 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    10441 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    10501 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    10561 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    10621 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    10681 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    10741 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    10801 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    10861 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    10921 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    10981 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    11041 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    11101 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    11161 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    11221 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    11281 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    11341 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    11401 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    11461 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    11521 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    11581 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    11641 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    11701 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    11761 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    11821 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    11881 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    11941 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    12001 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    12061 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    12121 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    12181 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    12241 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    12301 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    12361 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    12421 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    12481 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    12541 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    12601 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    12661 atggctatcc catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg
    12721 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    12781 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    12841 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    12901 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    12961 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    13021 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    13081 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    13141 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    13201 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    13261 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    13321 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    13381 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    13441 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    13501 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    13561 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    13621 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    13681 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    13741 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    13801 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    13861 cttcaaatcc tctttttctc accggttttc c
//
LOCUS       DACEND010000069        13436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEND010000069 DACEND010000000
VERSION     DACEND010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.071"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1030)
                     /locus_tag="IW746_001715"
     CDS             complement(74..1030)
                     /locus_tag="IW746_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1078615.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IW746_001716"
     CDS             1134..1736
                     /locus_tag="IW746_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1078616.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IW746_001717"
     CDS             complement(1824..3323)
                     /locus_tag="IW746_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1078617.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IW746_001718"
     CDS             complement(3339..3863)
                     /locus_tag="IW746_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078618.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IW746_001719"
     CDS             4161..4910
                     /locus_tag="IW746_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1078619.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IW746_001720"
     CDS             4901..5686
                     /locus_tag="IW746_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1078620.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IW746_001721"
     CDS             5655..6371
                     /locus_tag="IW746_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1078621.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IW746_001722"
     CDS             complement(6473..7492)
                     /locus_tag="IW746_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1078622.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IW746_001723"
     CDS             complement(7511..8569)
                     /locus_tag="IW746_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1078623.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8789..9937)
                     /gene="nagA"
                     /locus_tag="IW746_001724"
     CDS             complement(8789..9937)
                     /gene="nagA"
                     /locus_tag="IW746_001724"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1078624.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10013..10849)
                     /locus_tag="IW746_001725"
     CDS             complement(10013..10849)
                     /locus_tag="IW746_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1078625.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11033..12250)
                     /locus_tag="IW746_001726"
     CDS             complement(11033..12250)
                     /locus_tag="IW746_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1078626.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12633..12716
                     /locus_tag="IW746_001727"
     tRNA            12633..12716
                     /locus_tag="IW746_001727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12667..12669,aa:Leu,seq:gag)
     gene            complement(12956..>13436)
                     /locus_tag="IW746_001728"
     CDS             complement(12956..>13436)
                     /locus_tag="IW746_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078627.1"
                     /translation="FLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKL
                     SLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTV
                     WASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4044 a         2755 c         2314 g         4323 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca caaaaaaacc ttaaaaccaa acttaagaag
     8761 tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg ccatctagat
     8821 aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg cgtccttgag
     8881 cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga ctagccatca
     8941 tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt gcttctttca
     9001 atttcaatat acttccagct aagttgccag attctagacg tgctgttcct tcttttacta
     9061 ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc atcatacagt
     9121 ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc gcagcatttg
     9181 gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa gataataacg
     9241 cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa gcatggacaa
     9301 atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca ttactgtgtc
     9361 ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct acaccttgac
     9421 gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc cattcatgga
     9481 aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg tgttcttctg
     9541 tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct tcctgataca
     9601 ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc aatgttgttg
     9661 gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg atgccttctg
     9721 catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct accaaaccag
     9781 gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt agttctttgg
     9841 tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg acacctgacg
     9901 ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc tttgtttttc
     9961 tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc atttacaaat
    10021 tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct ctttctggtt
    10081 cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg taatagtagc
    10141 tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct tcgtcgattt
    10201 cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat tcactttttg
    10261 atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt
    10321 cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt tgtatttctg
    10381 tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct ttcaaataat
    10441 aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga taataataat
    10501 agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat tttagtactt
    10561 gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca aataatttat
    10621 cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt acccctagcc
    10681 gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt tcgatccgac
    10741 ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt
    10801 ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata agtatcctcc
    10861 ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc aaacttcaag
    10921 aaaagacttg caactaaata atatacttta gttatttaag aaaactccac tagaaagaga
    10981 gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa aatcattttt
    11041 ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa tcatccattt
    11101 taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca ccaccatgta
    11161 caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat
    11221 tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt tttacggctg
    11281 aaagaccagg aatatcattg attgcattat agatatattc tctttgttca tagatccttc
    11341 cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt tggataattt
    11401 gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc ccttctatat
    11461 agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga aatcctgcta
    11521 cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc gctaatgtag
    11581 caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg tcggaataaa
    11641 taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct tttggataaa
    11701 gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga ctcgttattt
    11761 ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa atataatgaa
    11821 caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc ggcattggaa
    11881 ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc aattcgctca
    11941 ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc agttggcaat
    12001 attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag ccttctgagt
    12061 cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg
    12121 cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc cgctccgctt
    12181 cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg gatttttcaa
    12241 attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc aaattttttg
    12301 ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta tttaggaaaa
    12361 acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg atagtcattc
    12421 catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat aaaaccaagc
    12481 aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag tcctgaaaat
    12541 tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga caactatgta
    12601 aaacctcgct atcattagtg tgttattatt acgcagatgt gttggaattg gcagacaagc
    12661 atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca tctgcactac
    12721 aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct ttatgccatt
    12781 atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt
    12841 gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta aaacaattaa
    12901 tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt gtatattatc
    12961 tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca aattgcataa
    13021 attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt agtactaaga
    13081 tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat actgtgggag
    13141 aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact aaaaaaatag
    13201 aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt aataatgcca
    13261 agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc gttcctaact
    13321 ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta tttccattga
    13381 cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt aagaaa
//
LOCUS       DACEND010000070        13213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEND010000070 DACEND010000000
VERSION     DACEND010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.072"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..289
                     /locus_tag="IW746_001729"
     CDS             <1..289
                     /locus_tag="IW746_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078628.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="IW746_001730"
     CDS             complement(386..820)
                     /locus_tag="IW746_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1078629.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="IW746_001731"
     CDS             complement(1052..1762)
                     /locus_tag="IW746_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1078630.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="IW746_001732"
     CDS             complement(1752..2612)
                     /locus_tag="IW746_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1078631.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="IW746_001733"
     CDS             complement(2617..3255)
                     /locus_tag="IW746_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1078632.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="IW746_001734"
     CDS             complement(3270..3569)
                     /locus_tag="IW746_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078633.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="IW746_001735"
     CDS             complement(3612..5096)
                     /locus_tag="IW746_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1078634.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKATCLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="IW746_001736"
     CDS             complement(5116..5577)
                     /locus_tag="IW746_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1078635.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="IW746_001737"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="IW746_001737"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1078636.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="IW746_001738"
     CDS             6880..7650
                     /locus_tag="IW746_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1078637.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="IW746_001739"
     CDS             complement(7923..8750)
                     /locus_tag="IW746_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1078638.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9318..10553
                     /locus_tag="IW746_001740"
     CDS             9318..10553
                     /locus_tag="IW746_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1078639.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11552..11812)
                     /locus_tag="IW746_001741"
     CDS             complement(11552..11812)
                     /locus_tag="IW746_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1078640.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11839..13140)
                     /locus_tag="IW746_001742"
     CDS             complement(11839..13140)
                     /locus_tag="IW746_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078641.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4188 a         2756 c         2079 g         4190 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagtggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca
     8821 aaaatgttta gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg
     8881 gcgtatctgc tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa
     8941 cacatagtga aaattgtcat ggcgagaaag tctaagaata tttttaaaca taactatttc
     9001 tttcttacgt gtgtataatg acgacagcac acctatcaat taatataatt attttatttt
     9061 tttatgttct catgtttatt tttaatgaca caacttctcg tatttatctg gcacaacata
     9121 taaatcgatc aaaaaattgt tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca
     9181 aaatttaaat ttagaagttg tgaaaacagc gagagacagt ttaatattca aaatatacat
     9241 aggctactcg aacgctgtgt tttttagtat actagtaaga aacgaaggga tagactcatt
     9301 ccaataagga ggatttcatg aagacaattc tttttgctcc tgcagtattc aatttagctg
     9361 agacaacacg aatgattgag gttgccaaag ctatgaaaga ccgttattac tgtgtattct
     9421 ttggcttttc taaaactttt gctcctttga tcacagaaag tggctttgag tatcatttac
     9481 tagcgccgat ccttacgaaa aaacaagaag aacaaatcat aaaatttgac cagttgcgta
     9541 cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg tatcactagt gaacagagtc
     9601 tgattgctaa gtttgagcct cgactgattg tgacaggttc gaatgtgaca atttttttat
     9661 cggcccgcaa taaaaagatt cctttagtat atgttaaacc ctatgctttg agtgaacctt
     9721 tttttcaatc agagcatgcg ctgatgcccc acgagttacg tttcttcggc cctttaagca
     9781 atcccctttg gcactggaca aaaaagcgta tagtaaatac ccgatggatc cccaatgcct
     9841 tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta caaatgatgg
     9901 atgctgatct aaatctcatc acaacgccta aactattttt ggaagaagaa cagctgccgg
     9961 aaaattatcg aatcgttggt ccaatttttg ctaagctcag tacttccttg cctgaagaag
    10021 tgctaacttt tatacagcga aagcaacaag aaagaaaacg tattatctat ttcgcaatgg
    10081 gtagttcagg caatccgcgt atgttacgca aaattttgtt cttcttacaa aaacaaccgg
    10141 atctagcggt tattgcacct attgactatc tgcttgatga cattcaatct gactcagaag
    10201 atcttttaat aacagaatat ctgtctagtc atcttttgca tgaattcatt gatttttctt
    10261 ttatccacgg cggggaaggg accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg
    10321 gtgtggggat gcaatttgag caaagctgta atattgacta ttgtgtaaaa tacggaaacg
    10381 caatcgccct cacaaaacca gtgactgaaa gaaaactgca gattgctctg cgcgaagttt
    10441 cttcacccaa gattcgtcag caggcacgca cgctgcaagc cgtttttcca ataaatggtc
    10501 cacaacaagc aattcaagaa atcgaaacct tcctgagcaa ccacccaata tagtttcaag
    10561 tagaaaaagc ttttttaata gagactcatc agtaatttcg ctattcgaga cccagacagt
    10621 gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga gttacttaaa ttacggtgtc
    10681 taaaaaacac aaaatcatcc ttaaaataaa agatctgacg ctatccaaat gagcataggt
    10741 agcgtctgcc atttattctt agtcaaattc tttgacacct ttctctggtt ggatcatctc
    10801 aacgtttctt caaaagaaag accataataa ttaggcaaat acattttctt aaaatgtgtc
    10861 atatagatct agaagcatcg ctttgtagag atttgtgccg ctagtcccct caacaattac
    10921 taaatcaaat gtaggattta ccactttatc tttgataatc aacctccctc ttctgttgag
    10981 acaatgatct cgcaatccga tgcatactcc tatataaact gccagcaatc cccacatatt
    11041 gagtagatca tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa gtcacgttca
    11101 ccatgactga ttgctattcc cagcacaatt atttctatac acgtttcatg atttgaataa
    11161 acactcacat catacatatt accattcata caccatactg cagcccctac tatatggttg
    11221 taagctccca aatctatatt tgacttgatc actttggtag tagatctact aattctaaat
    11281 ccattgctga taaaatttcc attttttcct ccttctttct gccaaaacag ttaaaacaat
    11341 aataatcttt atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc atattccgaa
    11401 taaacggtgg tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaaataagcc
    11461 aacatttcac aaaaatttaa gaagcaattt tcgtaaattt cttcttattt cttgaagcta
    11521 accacttctg ttacaaccac ttttagaaca tttaaagctt cgatgtgaca aaccatctat
    11581 gctgctcgat cataaaattg atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa
    11641 acaaaacatt catatgcggt acaatgatct ttggaagaat cacgatcaaa aatgatgcca
    11701 gtgcccaaat ccatcgattg atttgaatat gatatgtagc tagcatattc aatataaggt
    11761 gactagccgt taataaaaag ataaaaacaa aaaataaaat cagccaatac attgtttccc
    11821 accttctaaa aaccaaactt agacagtctg gacttcgttt tctccacgac tttgttcatc
    11881 tgacagcatc tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa
    11941 tgtgttgatc atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc
    12001 gattggagct ggtaatgtcc atgttggcaa aacagtaaat ctattgacca tttctaattt
    12061 gactgcaaaa taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat
    12121 gtaaggattc aacatcatag gagctccaaa aattatgggc tcattgatat tgaagatacc
    12181 aggaataata gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa
    12241 caaaacagat accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa
    12301 ctgttcagga gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc
    12361 attctcatcc aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc
    12421 atggatacca aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa
    12481 attattccct aaaatattgg ttaaaggctc gaataacgat aataaggcag cgtttacatc
    12541 aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa
    12601 tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt
    12661 tcgattttta aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa
    12721 catcccagat gctcccaaag aactcatagg taagacccag ccaagaggtg cctcttcagg
    12781 agttaaaacg tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc
    12841 tgtcacacta tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata
    12901 aatagccatc aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg aataaggggc
    12961 aataaattca ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat
    13021 caaaatcaat gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt
    13081 accaattttt gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac
    13141 taaaaccact ccttttcaat aaaatatgaa ccattcattg catgacaacg caaagaatgt
    13201 tcataactaa tta
//
LOCUS       DACEND010000071        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEND010000071 DACEND010000000
VERSION     DACEND010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.073"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..2674
                     /locus_tag="IW746_001743"
     CDS             203..2674
                     /locus_tag="IW746_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1078642.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2777..4513)
                     /locus_tag="IW746_001744"
     CDS             complement(2777..4513)
                     /locus_tag="IW746_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1078643.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4510..5214)
                     /locus_tag="IW746_001745"
     CDS             complement(4510..5214)
                     /locus_tag="IW746_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078644.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5345..5848
                     /locus_tag="IW746_001746"
     CDS             5345..5848
                     /locus_tag="IW746_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1078645.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5808..6146
                     /locus_tag="IW746_001747"
     CDS             5808..6146
                     /locus_tag="IW746_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1078646.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6167..7585
                     /gene="ffh"
                     /locus_tag="IW746_001748"
     CDS             6167..7585
                     /gene="ffh"
                     /locus_tag="IW746_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1078647.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7697..8275
                     /locus_tag="IW746_001749"
     CDS             7697..8275
                     /locus_tag="IW746_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1078648.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8279..8800
                     /locus_tag="IW746_001750"
     CDS             8279..8800
                     /locus_tag="IW746_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1078649.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8879..9433)
                     /locus_tag="IW746_001751"
     CDS             complement(8879..9433)
                     /locus_tag="IW746_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1078650.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9686..9961
                     /gene="rpsP"
                     /locus_tag="IW746_001752"
     CDS             9686..9961
                     /gene="rpsP"
                     /locus_tag="IW746_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1078651.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9973..10224
                     /locus_tag="IW746_001753"
     CDS             9973..10224
                     /locus_tag="IW746_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1078652.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10349..11140
                     /locus_tag="IW746_001754"
     CDS             10349..11140
                     /locus_tag="IW746_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078653.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11310..11831
                     /gene="rimM"
                     /locus_tag="IW746_001755"
     CDS             11310..11831
                     /gene="rimM"
                     /locus_tag="IW746_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1078654.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11821..12582
                     /gene="trmD"
                     /locus_tag="IW746_001756"
     CDS             11821..12582
                     /gene="trmD"
                     /locus_tag="IW746_001756"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1078655.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12718..13065
                     /gene="rplS"
                     /locus_tag="IW746_001757"
     CDS             12718..13065
                     /gene="rplS"
                     /locus_tag="IW746_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1078656.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4353 a         2410 c         2719 g         3664 t
ORIGIN      
        1 tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa cacaaaatat
       61 tatagaaccc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
      121 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
      181 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
      241 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
      301 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
      361 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
      421 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
      481 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
      541 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga
      601 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggata
      661 tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt
      721 gacaggcgaa gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt
      781 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
      841 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
      901 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
      961 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     1021 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     1081 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     1141 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     1201 gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     1261 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     1321 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     1381 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     1441 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     1501 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     1561 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     1621 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     1681 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     1741 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     1801 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa
     1861 tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg
     1921 cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact
     1981 tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa
     2041 tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact
     2101 gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag
     2161 tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat
     2221 cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat
     2281 gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt
     2341 ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg
     2401 ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt
     2461 ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt
     2521 gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt
     2581 agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa
     2641 aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa
     2701 aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac
     2761 gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa
     2821 gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta
     2881 accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct
     2941 caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg
     3001 atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga
     3061 cagcattttc aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg
     3121 gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat
     3181 tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct
     3241 cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt
     3301 tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta
     3361 aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc
     3421 caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc
     3481 ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct
     3541 gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat
     3601 gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat
     3661 agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag
     3721 catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt
     3781 ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct
     3841 gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt
     3901 gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca
     3961 gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac
     4021 ggcgaaaact ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg
     4081 cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag
     4141 tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg
     4201 gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag
     4261 tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt
     4321 tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt
     4381 ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt
     4441 gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt
     4501 ttttccattt catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa
     4561 gtactttggg tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc
     4621 tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg
     4681 gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt
     4741 tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg
     4801 tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg
     4861 tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata
     4921 gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag
     4981 catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc
     5041 catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc
     5101 taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa
     5161 cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat
     5221 ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat
     5281 tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca
     5341 gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa
     5401 aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc
     5461 agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg
     5521 attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga
     5581 agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct
     5641 agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt
     5701 ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat
     5761 gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa
     5821 agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa
     5881 tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat
     5941 acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg
     6001 attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa
     6061 atatgactgc atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac
     6121 agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag
     6181 cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga
     6241 agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa
     6301 cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt
     6361 gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat
     6421 cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat
     6481 gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt
     6541 aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc
     6601 gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac
     6661 tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa
     6721 cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga
     6781 gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat
     6841 gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg
     6901 ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc
     6961 agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt
     7021 attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat
     7081 tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg
     7141 ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg
     7201 cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa
     7261 cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc
     7321 agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg
     7381 ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa
     7441 aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg
     7501 ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa
     7561 gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg
     7621 tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa
     7681 cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata
     7741 tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat
     7801 gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt
     7861 cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat
     7921 cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt
     7981 ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct
     8041 ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc
     8101 aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt
     8161 tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct
     8221 tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat
     8281 gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga
     8341 tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta
     8401 tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt
     8461 ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt
     8521 acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca
     8581 aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt
     8641 ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg
     8701 gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg
     8761 attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag
     8821 gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt
     8881 actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta
     8941 atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg
     9001 cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt
     9061 ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat
     9121 cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg
     9181 gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct
     9241 cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa
     9301 atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa
     9361 aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa
     9421 ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt acaaaagata
     9481 agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat agattactta
     9541 tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa
     9601 gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat
     9661 aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc
     9721 taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg
     9781 tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa
     9841 agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa
     9901 catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata
     9961 aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc
    10021 acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat
    10081 ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa
    10141 gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat
    10201 atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaatattcg agtgcagctt
    10261 ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat
    10321 ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc
    10381 tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag
    10441 cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac
    10501 tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga
    10561 acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat
    10621 cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct
    10681 cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata
    10741 tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa
    10801 tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc
    10861 aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga
    10921 aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct
    10981 accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc
    11041 cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat
    11101 gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag
    11161 cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat
    11221 aattttggct taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa
    11281 aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg
    11341 taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag
    11401 aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat
    11461 taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc
    11521 cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact
    11581 ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg catgtattag
    11641 aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt
    11701 gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca
    11761 aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg
    11821 atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc
    11881 atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat
    11941 tattcagata acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg
    12001 ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat
    12061 gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca
    12121 gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat
    12181 gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg
    12241 ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta
    12301 gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa
    12361 tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat
    12421 cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg
    12481 cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc
    12541 aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct
    12601 ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta
    12661 cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg
    12721 aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc
    12781 cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag
    12841 ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta
    12901 cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt
    12961 gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt
    13021 gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac
    13081 tgagaaagaa cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct
    13141 gatcca
//
LOCUS       DACEND010000072        13105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEND010000072 DACEND010000000
VERSION     DACEND010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.074"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..975)
                     /locus_tag="IW746_001758"
     CDS             complement(64..975)
                     /locus_tag="IW746_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1078657.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1378..1956)
                     /locus_tag="IW746_001759"
     CDS             complement(1378..1956)
                     /locus_tag="IW746_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078658.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(1976..2644)
                     /locus_tag="IW746_001760"
     CDS             complement(1976..2644)
                     /locus_tag="IW746_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078659.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2659..3729)
                     /locus_tag="IW746_001761"
     CDS             complement(2659..3729)
                     /locus_tag="IW746_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078660.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3900..5264)
                     /locus_tag="IW746_001762"
     CDS             complement(3900..5264)
                     /locus_tag="IW746_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078661.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5261..6820)
                     /locus_tag="IW746_001763"
     CDS             complement(5261..6820)
                     /locus_tag="IW746_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1078662.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6876..9338)
                     /locus_tag="IW746_001764"
     CDS             complement(6876..9338)
                     /locus_tag="IW746_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078663.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGIPLQFDVTANVAKMTENGDKYDTSPN
                     KILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGA
                     YAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEG
                     RFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSP
                     VALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSK
                     GTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFA
                     YKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFT
                     LTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTI
                     HLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQEL
                     TLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLFKND
                     QGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSST
                     NAQPGATYQGMVTWELTAGPTS"
     gene            complement(9322..9657)
                     /locus_tag="IW746_001765"
     CDS             complement(9322..9657)
                     /locus_tag="IW746_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078664.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9671..10705)
                     /locus_tag="IW746_001766"
     CDS             complement(9671..10705)
                     /locus_tag="IW746_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1078665.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKVETT"
     gene            complement(10795..11475)
                     /locus_tag="IW746_001767"
     CDS             complement(10795..11475)
                     /locus_tag="IW746_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1078666.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(11759..12862)
                     /locus_tag="IW746_001768"
     CDS             complement(11759..12862)
                     /locus_tag="IW746_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1078667.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         3763 a         2696 c         2247 g         4399 t
ORIGIN      
        1 cgataataag cccggccaaa cgaaaaatat gaggagctat ttttgtttgg ctgggcttat
       61 ttattactga aaataagtgg tcaatgcttg tgtcaaactt gcagcaatct gctgctgata
      121 actgtctgtt actaattcct ttaaatcact atcattattc atataaccca tctcgattaa
      181 aatagaaggt tgaagatttt ctcgaagaac ttgatagttc ccagtggaga ttcctcttga
      241 atccagaggc agatcagtga aggagtcagc aattgtctgt gctaatgttt cctcttgtcc
      301 atagtaataa tatgtcgtga ttccagttgc ttcattcgca tattccgtag agtctgcgtg
      361 taaactgata aagacatctg cctttttttc ttcactgtag tcgcatatat ctccaagctg
      421 tactaattca tctgtagtat gggtcaaata aaccgttgct ccagcatcct ctagcgcctt
      481 ttttactttt atggctgtac ttaatgttat atctttttct tctgtttgtt catcattgct
      541 taatgcccca gtatcatttc cgccatgccc aggatctaat acaatcactg catcagatag
      601 ctcttccggt tttgtttgta tattctttgt gactttggtt ccatcttcga gggctaaata
      661 tccagtatag ccatcatttg tcttgatctg ataagcattt tttgtttcag aaagaaactc
      721 cgccagctct ccgccgttta tagttgcaag tggggtagtt agcgagtcat ctgtatatag
      781 tgtacggtca ttttttccga ttttgattaa tgtctcttgt tctgtatcat caaatgattc
      841 ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt tgtccaacta tctttttttg
      901 accaaaaaca tctatttgaa aacagataat aatgataatg attgcaaata caaaagctcc
      961 aattattttt ttcattcttg actctcacct cgtagatcca gactaagggt caaaacttaa
     1021 aatcagctta agtacgcaaa aaaggggctg taaaagatat aaccgtagaa aataacatta
     1081 ctgctaaaat gatttttttc atttttggca tatcctgaaa tctccttttg actattcgtg
     1141 gtgattcatc tacccaataa atagtttcct tcattctaca aacaattaat ttcttcgtta
     1201 ttaaattaaa acacttcctg gaacaccgac ataataaata aaaatatacc ctgataatca
     1261 tgagagaata acaaatttgt atttatgaaa aactacctat ataaaaaaaa caagatataa
     1321 cgatcttcgt catatcttgc ttcttcacct aactattttt ctccctatat aacagcttta
     1381 cgcttctcct aatacaccat tcaaatataa atcgatcaat tgcgccacat ttagcggttc
     1441 ttccacaggt cttttcgacc gagtgatgta aggtgctaac actaagaaaa aatgccttgc
     1501 tgcgatttct tttgaaatcc catttcttag cacagattgg tttgctgaaa agatttcttc
     1561 aaaaggttca cgataatttt tcttccatag aaagaaaaga tgatctcttg tttcctttga
     1621 aagattgctt tcaagatcgt gaagcatcag aaacaaatcg accagatgtg tctcaatcaa
     1681 aaactgcgcc atttttgtca aggttacttg aaatcccgtt tcgcttgttt gaacgatttc
     1741 tctcaaagct tttccaacat cttttaagtg gttttctaaa actgccgtat atagtttttc
     1801 tttatcaccg aaataatgat agagattggg ttgggtgatc ccaacttttt gtgcgatctc
     1861 tcttgttgaa gtgccgagat atcccttgga cataaacaag tcagtcgctt ctttcaaaat
     1921 tttttgctgt gtatctttgt tgtatcgatg gaccattttt ccacctgctt tttggttatt
     1981 ctttttcttt caatacacca tcttccatca taaatattct atcgcatttg tcaatcagcc
     2041 gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc ttttgattct tttgccaaga
     2101 tatcgactac ttctcgtgcc ttttctgaat ccaaactagc agttggttca tctgctagaa
     2161 taatggacgg atcattgtac aatgcacgag cgattgcgac cctttgccgt tctccaccag
     2221 atagttcttc tggatacttg ttgatcaact tatccacgcc aagctgctca aataattctt
     2281 ttgcttcttt ttgacgatgt tcatgtttga tcttatctac taataataac tgctttttta
     2341 ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa gccaatttct ttaaaacgta
     2401 gtttaacgcg ttcactttct ttttgattac tgaattcttg atcattgata aatacagagc
     2461 cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa agtactttta cctgaaccag
     2521 aaggaccgat gattgcaaca aattcccctt tatcgatact catggttgtt tctttcaatg
     2581 ctttaactac ttggtcgcca tcttggaaac ttttttctac ttctttaaat tctattgctt
     2641 tcatgctctt cctccttctt atccaattgc cttcaacgga tcgattttta cgattgtccg
     2701 aacagaaaat agtgcgccta acacagctac taccagcatc aacaagctaa tgacgccaaa
     2761 aaacagccaa ttgctttgga atggaaccgc atcaggtaga acaagggatg ttcccactgt
     2821 tcctaataga ccaagaagaa taccaacaaa tgataaaatg aatgtttgtg ccactacaga
     2881 acgcgcaata aatcctcctg tgatcccttg tgctttcatt acaccaaaga tattaatctt
     2941 ttgcatcgtc agtacataaa tgaaaatccc gatcacaata gccgcaataa cgatcaaaaa
     3001 tccgatcata aatccaaaag tcaatacttg cgcattatat ccaggtaatt cattgatgaa
     3061 tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta ctgattttac ctctgacaac
     3121 tattgcgtta atccgtgcat tttcacttgt atcttctttt tcgaagcgga tctcttggta
     3181 agcattgatg gttgtataga gaactggtga gacattgaat ttggcatgat ccgtaaagcc
     3241 agttagttta aacgtcttgt cactaccaga taacttaact gtatctccta cggctagacc
     3301 atattcttct ttcaaactaa tatcgcctac tgcctcatca tcgttatcaa aagcttttcc
     3361 ttcaacaaga ttgggcatga tgaattgatt tttatcgata ccgaagaagc taacattgat
     3421 ttttccttct tctgtactgt ctttactagt cacgactcca ggtgtctggg caagatacgc
     3481 cactttccca ccttcgactt cttctgctat cttcttagta atcatggaca tccctagatt
     3541 tgaatttgca tctttcgaaa gcacaatcga atctgcctcc cacttgtcga ctgctgttcg
     3601 attatcttga gctaagccat aagctaaccc agtcaaaaaa aataccaggt aggcgatcaa
     3661 aaacatcacc cctgcaacta acgcataacg taactttgaa tgcataattt catttaatgc
     3721 taaaaacatt taactcctcc tcctgaaata atcattttat acaacaattt tatcacttga
     3781 taaatttttg gcgaatatta tgctcaaaaa attcttccgc tatttcatta tatgaatttt
     3841 ttattctcga ttgattaata tttattatat ccccacacaa taaaagaccg tgatatttct
     3901 cacagtcttt tatctactct attgctattt gctctcttag ctagtacttt aaaagctaac
     3961 tcattgaggt agtgaaaacg agaggtgtgt tcagacagaa atccaaaatc ctcaactgct
     4021 agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa gcaggttcaa atctgataat
     4081 aagaattctt ggcttatcag ctgtccgcca attatttcta atgtatccgg atggtagatc
     4141 agctttgcat ttactgtttt gttttcttca tcgtattgtg gaaatagatc aaattctcct
     4201 gaacacgaac cggtattttg ttccaagaat ggcgcttcat cttctgtcag ccctacagtt
     4261 cctaaaaagt agtctataat gactgctgct gttatctttt gtctgaggtc gtaagcggtt
     4321 ggctgaccac tcaaagttag cgctactgct cttgccatat gtacagctcg agtaactaaa
     4381 ggtaaaaaga gatgccgttt atttttgtca aaactgactg ggacgatatc tccaatcgca
     4441 tagatgtccg gatcacttgt ctgcagatat tcattgacta tcacagtctc atcctcatac
     4501 aaagccactt tttcttttaa caatgtcgta ttaggacgag tttgggcaga aaaaatgacc
     4561 atatctgcct ttattgcttg agatagaagt tgtacggata ctactttctc ttctgatttt
     4621 ttccaatcga tcaagaattc atttggataa aaattgattt gcttagtttg cagctgattt
     4681 tgaaaactgt tgatcatatc cttatcgaaa tatcttgata aaacattcgg catataatca
     4741 actagatgaa taatgtatcc tttatttctc aaagcatctg ctaattccaa tccaatataa
     4801 ccagcaccga agatcactac ttcttttgct tcttttaaag cggcaagtac ttgcttagat
     4861 tgcactagat ttttataggt gtagacattt tcagcttcaa tttgtttata caaggtgaca
     4921 ggagaggaac ctgttgctac gattaattta tcataggtta tggtctcgtt ttttccttct
     4981 ttggatgcga ggatgagctg atgtttgtct ggatcaagtt cagttactct ccattcggtg
     5041 atcagttttg ccccactttc ttgaagagtc cgcatgttat gggcaacttc agctaaacta
     5101 cgtatctttc cttttaaata aaggttgatt ccatttgaaa catagcctag tccatcttta
     5161 tgatcttcat cgatcagtaa aacttctgct ttcggttgaa gttttcttag atttagggca
     5221 gctgtgatgc ctgcatggga agcaccaatg atcacgattt tcatgtttta ttcatcctta
     5281 aattcaattt ttctaaataa cattctttta aaaattggtt caaatagggg aaatcataat
     5341 tatattcgtt gctagtgaat acaaaaactt tagcggactt ctgtgacggg tactctttca
     5401 attggctagt caaaatgata tcatactgct tttctttctc gtattcgact aaatcatatt
     5461 tgatcgtatt cagcaattgt tcctggatga tttctttcac gatatccgcc attagccttt
     5521 cataaggaag atcataagca atttgcagac gtaattctaa ctgcttgatc agcgtccgca
     5581 aaatagatgc atagcgagca aataattcaa agcgggcatt ttcctcgtct acttcagtaa
     5641 aatcaacaat agcaaatgtt tgtctgacca atctttcaca taaaaccagg aattgtttat
     5701 cttctacttc agttaaccgc tgttttaatc cttcttggcc aaaaatattc atccatcctc
     5761 taaaataggc aaaatgagcg tgattttgca tcactgtcaa cgcaatcttt tgcgaaatct
     5821 cttctggaaa aacataaaaa gagaaatatt cttctatcga ttgtttaaac gttcgattca
     5881 attgagctac tacgtcgtct ttgtattttt cgcttatcaa aaactcctgg acatgcgagt
     5941 tatgtgtatc taatgtaaag ttactgatca tgaaaagata aaaaaccaac gtttcatttt
     6001 gattccaagg aaaggcatat tgaaagaagt aacgggtcaa aaatgtatgg aggtccaata
     6061 aaaattgtgt cgtttgaaac aatccaacag gaagattgct ataatctggt ttggattttt
     6121 tcgtacagcg cattcgcttc ttcataatgg aaagccatat ttcaaatttc aatcgtcctt
     6181 cttctgtaat agtaaaacta aactcttttt gcagcagatc taaaaaagtg tttcctgata
     6241 aatcactgta ttcttttttg ttttcaagat aaggacgtcc aaaccaaaat aattggaaat
     6301 aaaaataaca aatttgactt tctggaccgg tcagccgccc gctttttatt ttcagatgaa
     6361 actcatttaa agaatcattt aattccttca ctctgcgaga caaagtagcc gaactgatat
     6421 taagttccat gacaagtttc tgccgatcaa atgagccatt ttccaacaga taaagaagta
     6481 tctgatattt tgtagaatag cgcaagtagc aatgataata aacatcgact gctgctatat
     6541 ttccatgtct gattaataaa atattccctt cttggataac aatttccaat tgtttgatat
     6601 tttccaagtt agcttgtaaa taaaacagat cttctttcaa ggtagcccga tcaactgata
     6661 agcgggtaag tacgttatta acggaaatct ctcctttttc ctctaataaa agttggaaaa
     6721 gtttgatttg ccgttgttca tttttttcaa gcaaactgaa aagttccaag tccactcctc
     6781 caagcatttc tgattttaac agcagaagtt tccttctcat ccttgaaaaa gaggatctgt
     6841 gacagttatc acagatccat acaaattcta atcacttagc tggttggccc agctgtcagt
     6901 tcccatgtga ccatgccttg atacgttgcg ccaggttgag cattcgtact gctatttacc
     6961 tctaagagta aaccatcggt atcgggaaag tcaacgagaa cctctccttt agctgttcca
     7021 tcactctcat aaatcaacgt gttttgacct tgctgaatca attgttttga tccagaatga
     7081 ttgaagtata attttgttcc gcttaattct tgaccttgat cattcttaaa taaagaagtc
     7141 aatgtccctg tcagttgcca aggagacgtg cttggaagcc gagtatcccg cacatcaaaa
     7201 gaaaaatcgc cttttgcttc ataataactt gtcttcaacg gtttggggag atcgttacca
     7261 aatgtaaaat catctggtac attaactaat gttaattctt gtttggggac aagaattgtt
     7321 ttagcagatg tttggacaac tgggttagga agcttagcac catctgctaa tgtaatattg
     7381 ctggaaagta cagctgcctt tgtcagtatg ctgacggatt gtacagaagt atctacagta
     7441 tttaattgat agttgtaagt aagatgaatg gtatctccta ttttgctcaa tagatctgct
     7501 gggacagaaa ctccttgttg aatgccggta tctgcgatag ttgtaccatt taattttaag
     7561 gaaccattga tataagtagt attttcagga agtgagatcg tgattttttt gtcggttatc
     7621 gcatccccag attttgttaa cgtgaatgtc acattttctt gaatcgtatc tcctgataga
     7681 gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt tttttagctg actatctgca
     7741 ctcacagatg gaaaattagc cactgttatt tgaggaagag tggtgattcc agcttgttca
     7801 gactgccatt tgtacgcaaa agacggatac atgttagtaa gtacatcgtc caccaagcga
     7861 taatcaaaat aatattgccc tgaagccaat gcaggtagtg tagaagtagc tgtatttaca
     7921 cccccgttat ttgattggat cgtttggaaa agttgatctg tccatcctgt cgctaatttt
     7981 tgggttcctt tcgaataaag cctgtaatgg atcgaaccct tttttagttc agttgctgac
     8041 caattggccg tgacttggta cgatgcagaa gaaaggagct ttccccactg actatcggct
     8101 gttaagtttg ttggtaaaac cgttccgtct agcttgtact gctccaatac ctttaaagca
     8161 acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt acacgatatt ggctgtctga
     8221 ttcaatccac ttgtgcttgc tggagtatga gtcagtattg ctccttgtgt aacgtttttt
     8281 cctgtaaaat agttgactac gcgatcaaag cgactaaatg gcaactgatc aaaacgtcct
     8341 tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc ctggaaggtt ctggttattt
     8401 ccatccacgt attctgtggt atcagcaaat gtattatatt ttcctgttga atcacttgtt
     8461 gagatgtaac tattccacgt atttatctct gcacttgcag aaaatgcata tgctcctgga
     8521 ttcaagtttc cgccagttaa tttaggataa aatctacctg cagctggagc taaaatcgaa
     8581 ttgaacatac gatcttggat aatattattc gaatcataaa aatcaacaga aaaaacggcc
     8641 tttttagaca tagaaggttg taatttaccc ttggtcaaaa ttttgtttgg actcgtatca
     8701 tatttatctc cgttttctgt catcttcgcg acattagctg taacatcaaa ttgaagcggg
     8761 atacctcgcg taataatagc aataatatct gccaatagag tatttgtatc ccacgacccc
     8821 atcccaaaac gcaaggtatg gcttcctgct gataataaac taccgctagt aaccttgatg
     8881 ggggcaccgg caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg
     8941 cttgatacat gttccggcaa attgatgatc gccacaaatg caaatccttg tgcagtgata
     9001 ttcaccaaat caagtacatt tgttagtttc acatggcttt cccaatgaaa acggaccgtg
     9061 ccacctttcc aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat
     9121 tggttagccc cagttagttg gacaagcttc ccgccattga ctgcactacg tccaccattt
     9181 ggaggtacga tattttcata cgtgtatgga tcataagaaa tggcaggagg cgttgctcca
     9241 acagcttctg ccgaaggtga aaagataaat atacttaaaa caaaaagcga agtaaaaaag
     9301 aaggagcgaa aaaatgaaaa tttattttgt agcaacatgt tcatcctcct tttcctttct
     9361 tctccataac aacaaccacg caagaagcca taattctata ccaatgatca aataaagaaa
     9421 acggatcttt tcttcattcg tctccgggaa tacgccatgt tgattagatt ctttataaat
     9481 cggtagctgt gtctgctgtt ttgatgcgcc attgactgaa ccacttggat gcggaactgg
     9541 acttagatca taaaatgtaa tcgtacctga acttttggta tctgcttgga ctggctcttg
     9601 ccagctgaag aacagcaaac ttgcaataat ccctcccaat aagatcgatg ttttcaacga
     9661 aatcacctca tcatgttgtt tctacttttt tctttttctt gtggacccac aaaaacagca
     9721 aaatcaacca taacactaga actgcagcaa ccaatcctgc ataaagccat gggaaagatt
     9781 gttcttgtac tgcaaaaacg gaattttcat ttactttttg tgctttttct tttttgatcg
     9841 taaagttttg tgtaaatcgc cattcattac tgtcgcctga ttttgctatg atttctgcgc
     9901 ggtaatcgcc ggcttttgct ttatcaccag ctaaatcaag attcaagtga aaattcgaat
     9961 ttggtgcaac taaaaattca ttagacgtat tttcaatata aagctgattt tcataaaaga
    10021 tcttgctcgt aaatatcaaa ttgctgataa tcctaggctg aggattttgg aaacgaagct
    10081 gaacagttgg ctttgcgttg acctcgtcca aatctcctga taaaagtttc agatccggtt
    10141 ctatcgtctc ttgactttct tgtaaaacga ctgcgagatt ataagcaata cgatttttga
    10201 tttgtgctcc ttctgtagca ttttctgttt cagcttcttc tttaggtgaa acatgaatac
    10261 ccgctaatac acgtccctta aagctttttg ctgggatttt cacttctata ggtatagttg
    10321 tttctgcatg tgctgataca ttgatttcct ttttcgcaat gagcaaaacg tcacggacat
    10381 caaacggcaa tgtctcatct aacgttgggt ttttttgact ataatcgatc gttccaccat
    10441 cactcgttac cgccggattg acagagacct caaatgttcg ttcttcatct gaagaattgc
    10501 tgataattac ttctagtgtc tgattttgtt ccggtgccac taataaatca aaataaccga
    10561 ttgctttatc tatttggttc tctggaaata ccggtttgat tccaaagtct cctgctgaat
    10621 cctttgcttg gctgattgac ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa
    10681 caaacagtcg tttactttga ttcattgtcc accacatcct ttcaacaaca actgcggtaa
    10741 aaaatccttc acctatagta ttttcaccgc agtttgttga atttatttca tactctatgc
    10801 tactgggcca gccacgaggt tccaagtcaa atcagccgtg aatgttccag ctgctgcttt
    10861 cggcactttc agtgtgatat ttgctggatc ataaacagaa agccatgttc ccagaccttg
    10921 atcttttgct gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc cagttacagt
    10981 ttgactagtt ggtccagtgg attcgttatc tgacgtactt ttaacagtgg atgttccaaa
    11041 atctaattca gcgccttgca aaacttgtga accgtttaca aaatctgaaa ccgaaactgt
    11101 cacgttccac ccttgtgctt ggccatctgc acctacacct cgacgatctg atacttggag
    11161 ataaggtgta tcgttttttg aagcgtctac ttgatacgtt tttgttccgc tttcaatctc
    11221 gtgtgtacca aatggcaatt caggaactac atccaatgtt aaagaacctg tctggccagt
    11281 tgctggattt tccggatctg ctggctctac tggttttgaa ggatcagtag ggttaagagg
    11341 ctcaacggta tttgttggtt cagtaaaggt aatatctcct tttgaagtcg cttgaccctc
    11401 agcaaaagct ggtgttgcca agccgcctag aagaagtgtt gaaagtaata atgtcccaat
    11461 catgttcttt ttcataataa atcactcctt tttcttagtg acctaagtat agtagagaca
    11521 gtatgcaaaa aatcctcatg aattcatccg cttacttgaa attatgaccc tttttgtata
    11581 aaagagaaaa aaacaataaa atcgtttaca tgaatattct tcctttttta ttaagtatga
    11641 aacaatattt caattttatt ttttcgtttc aaattattct attagtgaca tccttgaact
    11701 ataaaaatag gagtacggca tcatctgccg cactcctatt tttttcgtca tttatttctt
    11761 aaataaaacg attctcacgc aattttattt ttttgatatc ttctactgtt tgtgttccag
    11821 ctaattgcat gaccatttcc aattctgttt taaagaaatc aaacacttga tggactcctg
    11881 tacttcctcc taaagacaat ccataaataa cgggtctgcc aatagcaaca agatcggcac
    11941 ctgatgcgat tgctttgaaa acatgttgtc cacgtcgtac accgctgtca aacacgattg
    12001 gtacacgttt atctactgct tctgccacat attgcaacga atcaaacgca gcaggccccc
    12061 catctaattg acggccacca tggttagata cccaaattcc accagcacca gattctaatg
    12121 atcgatagac atcttcttct gattgaacac ctttcacgta gactggcaaa tcagaatgag
    12181 cagcgataaa ttctacatct tttggactaa gtttttgttt ggaagattta tatacagcat
    12241 ccatcgtttg gcctactcct gattgataag cctgaacgat cggcattggc agcgggaagg
    12301 taaacccgtt tctgcggtct gtttcacggt ttccgccaac tgttgcatcc gctgtcaata
    12361 cgattgcttt tgctccatta cgcttggcca cttcaagaat atccaaattg attccattat
    12421 ctttgctcat ataaaattga aaccattgcg gtgctttctc cccgcctgct tctcttatct
    12481 cttctaaagt acaagaagcg taagaacttg ctgtatagat cgtcccaaaa tctgctacac
    12541 cttttgcaga agctttctcc gccttcacat gagctaatcc atgagcagca actggtgcca
    12601 tgatgattgg tgcagtcagc atttcttcgt caaaatgtgt agttgtatcg ggtaattcta
    12661 catctcttaa tacatgagga ataatcaaac gatgattgaa tgcccgttca ttttcttgat
    12721 aagtaaataa gtcgccagca ccactgctga tgtaaccata accaccagct ggaatcactt
    12781 gtgaagccgc gatttctaaa tcttccatat tgataaaatc tactattcct tcatttgtac
    12841 ctgcttgata tgttttttcc atctcaatgc cttctctcta taaaatttga atgatgaaca
    12901 atatgatagc gtttacactt tttatattac tcttcgacat tgatacaggc aaataatctg
    12961 tttacatttc agccattcca gatgtcacta ctctggaaga aatgattgta ataaaaatag
    13021 ataaaaaacc gatgggactt tgacaaaagt agtgtcaaag tccctttttt tgaataaacg
    13081 gtgttcaaaa gccattttct cggta
//
LOCUS       DACEND010000073        13049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEND010000073 DACEND010000000
VERSION     DACEND010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.075"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW746_001769"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW746_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1078668.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW746_001770"
     CDS             1873..4539
                     /locus_tag="IW746_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1078669.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW746_001771"
     CDS             4903..5655
                     /locus_tag="IW746_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1078670.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW746_001772"
     CDS             complement(5677..6831)
                     /locus_tag="IW746_001772"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1078671.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IW746_001773"
     CDS             7016..9457
                     /locus_tag="IW746_001773"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1078672.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGEKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IW746_001774"
     CDS             complement(9787..10491)
                     /locus_tag="IW746_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078673.1"
                     /translation="MKKKQKQELLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IW746_001775"
     CDS             complement(10678..10923)
                     /locus_tag="IW746_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078674.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IW746_001776"
     CDS             complement(10940..11791)
                     /locus_tag="IW746_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1078675.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IW746_001777"
     CDS             11957..12961
                     /locus_tag="IW746_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1078676.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4168 a         2316 c         2717 g         3848 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcga
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattc ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DACEND010000074        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEND010000074 DACEND010000000
VERSION     DACEND010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.076"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IW746_001778"
     CDS             complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IW746_001778"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1078677.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IW746_001779"
     CDS             complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IW746_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1078678.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2943..3506
                     /locus_tag="IW746_001780"
     CDS             2943..3506
                     /locus_tag="IW746_001780"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1078679.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3759..4280)
                     /locus_tag="IW746_001781"
     CDS             complement(3759..4280)
                     /locus_tag="IW746_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1078680.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4283..5125)
                     /locus_tag="IW746_001782"
     CDS             complement(4283..5125)
                     /locus_tag="IW746_001782"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1078681.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5118..5843)
                     /locus_tag="IW746_001783"
     CDS             complement(5118..5843)
                     /locus_tag="IW746_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1078682.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6019..6315
                     /locus_tag="IW746_001784"
     CDS             6019..6315
                     /locus_tag="IW746_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078683.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6883..8475
                     /locus_tag="IW746_001785"
     CDS             6883..8475
                     /locus_tag="IW746_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1078684.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8488..9228
                     /locus_tag="IW746_001786"
     CDS             8488..9228
                     /locus_tag="IW746_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078685.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9396..10745
                     /locus_tag="IW746_001787"
     CDS             9396..10745
                     /locus_tag="IW746_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078686.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(10873..11463)
                     /locus_tag="IW746_001788"
     CDS             complement(10873..11463)
                     /locus_tag="IW746_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1078687.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11610..12962
                     /gene="brnQ"
                     /locus_tag="IW746_001789"
     CDS             11610..12962
                     /gene="brnQ"
                     /locus_tag="IW746_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1078688.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3631 a         2581 c         2573 g         4221 t
ORIGIN      
        1 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
       61 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
      121 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
      181 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
      241 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
      301 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
      361 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
      421 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
      481 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
      541 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
      601 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
      661 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
      721 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
      781 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
      841 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
      901 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
      961 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
     1021 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
     1081 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
     1141 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
     1201 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
     1261 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
     1321 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
     1381 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
     1441 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
     1501 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
     1561 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
     1621 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
     1681 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
     1741 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
     1801 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
     1861 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
     1921 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
     1981 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
     2041 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
     2101 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
     2161 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
     2221 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
     2281 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
     2341 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
     2401 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
     2461 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
     2521 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
     2581 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
     2641 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
     2701 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
     2761 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
     2821 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
     2881 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
     2941 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
     3001 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
     3061 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
     3121 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
     3181 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
     3241 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
     3301 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
     3361 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
     3421 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
     3481 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
     3541 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
     3601 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
     3661 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
     3721 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
     3781 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
     3841 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
     3901 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
     3961 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
     4021 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
     4081 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
     4141 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
     4201 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
     4261 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
     4321 gtttcatcaa atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca
     4381 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
     4441 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
     4501 ccaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
     4561 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
     4621 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
     4681 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
     4741 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
     4801 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
     4861 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
     4921 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
     4981 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
     5041 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
     5101 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
     5161 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
     5221 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
     5281 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
     5341 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
     5401 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
     5461 tttcgacaat gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc
     5521 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
     5581 tgtgctattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
     5641 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
     5701 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
     5761 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
     5821 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
     5881 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
     5941 gcaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
     6001 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
     6061 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
     6121 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
     6181 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
     6241 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
     6301 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
     6361 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
     6421 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
     6481 ttttcaaaaa ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
     6541 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
     6601 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
     6661 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
     6721 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
     6781 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
     6841 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
     6901 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
     6961 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
     7021 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
     7081 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
     7141 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
     7201 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
     7261 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
     7321 gcaaaaagtt tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac
     7381 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
     7441 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
     7501 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
     7561 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
     7621 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
     7681 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
     7741 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
     7801 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
     7861 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
     7921 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
     7981 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
     8041 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
     8101 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
     8161 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
     8221 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
     8281 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
     8341 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
     8401 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
     8461 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
     8521 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
     8581 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
     8641 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
     8701 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
     8761 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
     8821 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
     8881 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
     8941 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
     9001 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
     9061 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
     9121 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
     9181 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
     9241 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
     9301 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga
     9361 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
     9421 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
     9481 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
     9541 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
     9601 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
     9661 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
     9721 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
     9781 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
     9841 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
     9901 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
     9961 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    10021 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    10081 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    10141 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    10201 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    10261 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    10321 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    10381 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    10441 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    10501 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    10561 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    10621 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    10681 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    10741 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    10801 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc
    10861 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    10921 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    10981 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    11041 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    11101 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    11161 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    11221 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    11281 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    11341 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    11401 aataactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    11461 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    11521 accatcgttt tatataggga attttctgaa aattgttgca tatttttcga aattttgtta
    11581 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    11641 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    11701 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    11761 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    11821 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    11881 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    11941 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    12001 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    12061 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    12121 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    12181 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    12241 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    12301 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    12361 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    12421 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    12481 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    12541 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    12601 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    12661 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    12721 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    12781 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    12841 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    12901 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    12961 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaa
//
LOCUS       DACEND010000075        12966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEND010000075 DACEND010000000
VERSION     DACEND010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.077"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..357
                     /locus_tag="IW746_001790"
     CDS             79..357
                     /locus_tag="IW746_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078689.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            376..1584
                     /locus_tag="IW746_001791"
     CDS             376..1584
                     /locus_tag="IW746_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078690.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1732..2097)
                     /locus_tag="IW746_001792"
     CDS             complement(1732..2097)
                     /locus_tag="IW746_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1078691.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2244..3017)
                     /locus_tag="IW746_001793"
     CDS             complement(2244..3017)
                     /locus_tag="IW746_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1078692.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3191..4051)
                     /locus_tag="IW746_001794"
     CDS             complement(3191..4051)
                     /locus_tag="IW746_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1078693.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4051..5469)
                     /locus_tag="IW746_001795"
     CDS             complement(4051..5469)
                     /locus_tag="IW746_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1078694.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5526..5975)
                     /locus_tag="IW746_001796"
     CDS             complement(5526..5975)
                     /locus_tag="IW746_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1078695.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6420..6683)
                     /locus_tag="IW746_001797"
     CDS             complement(6420..6683)
                     /locus_tag="IW746_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1078696.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6782..8077)
                     /locus_tag="IW746_001798"
     CDS             complement(6782..8077)
                     /locus_tag="IW746_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1078697.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8074..9345)
                     /locus_tag="IW746_001799"
     CDS             complement(8074..9345)
                     /locus_tag="IW746_001799"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1078698.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9510..10379)
                     /locus_tag="IW746_001800"
     CDS             complement(9510..10379)
                     /locus_tag="IW746_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1078699.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10671..12281)
                     /locus_tag="IW746_001801"
     CDS             complement(10671..12281)
                     /locus_tag="IW746_001801"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1078700.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3670 a         2741 c         2233 g         4322 t
ORIGIN      
        1 tcacttatgc gatttttttc ctttttttta ttcgttatac tgcagaagta aaatttttta
       61 agaatagagg gaaaaaacat gaattactgg atgaacacaa tcattaatcg attagaaaca
      121 gcatatcaaa caagatttga tatgaaagct tctttagtct ttttaaatga tgcgtatcaa
      181 aactcgatcg aattgatcaa agcagtggat gagaatccaa caaacgaatg cgaagaattt
      241 ttaaatctgt ttatgtctac aagagattta tttattcgcc aacttgttga tcgctatcca
      301 tccaattatc acgatgtaga agttcaaata caaaaattaa aagcatattc agcttaaggg
      361 gaatgtataa catacatgga aaagaaatta tttaataaat attttattgg aatcacattg
      421 atcaatttta ttgtctacct gatttattat ctgttgatgg tcatcatggc agtcattgcg
      481 caagacacct tacacgccag cttgagccaa gcaggttttg cttctgggat ctatatcatc
      541 ggaacattgc tggcacgctt attcatgggg aaacagttag aactatttgg tcggaagttt
      601 accttaagag gcggcgcgat cttctatctc ctttcaacat tagcatatct ttatacgcca
      661 actatcgggg cattgtattt agttcgattc ttgaatggat tcggctacgg tacagtttct
      721 acagctacaa atgctatcgt tactgcatat atccctgcat ctaaaaaagg ggaaggcatc
      781 aattattatg gattaagtac tagtttagct gccgctgttg ggccattttt aggaatgatt
      841 ttgctgaata tgactgattt tcgtttcatt atctggtttt caattgtact ggtcttcttt
      901 gttacgattg cctgccttgt ctttcctgta aaaaatattc cattatctga agaacaaaaa
      961 atatctcttc gctcatggaa tatcaatagt tttattgaaa agaaagcact tttcattaca
     1021 ggaattgcct ttttgatggg tctttcttat tcaagtgttt tatcattttt atcttcttat
     1081 acaaaagtaa tccacttagt tgctgctggg tcatttttct tcgtcgtata tgctttggtc
     1141 atcacattta cacgtccatt gactggaagg attttcgatg tcaaaggaga acaatacgtg
     1201 atgtatccta gctatttatt cttaacagca ggattatttt tactgagtgt aacgacgaat
     1261 agtgtgacat tgcttatctc tggagcatta gtcggcctag gttatggtac gtttatgtca
     1321 aatggacagg ctgtttgttt aaaaatcgtc cgcgaacatc gaatcagtat tgctttatct
     1381 acctacttta ttggtttaga cctaggctta ggcgttggtc cttatttgat ggggatgcta
     1441 aaatctctta cttcatttag aggattgtat gtgattgccg gagtcattcc attgatatgt
     1501 acagcactct atcttgttaa tggccgaaaa catagtcatg aagcagaggg cgaattgatt
     1561 gaatcattga aaaatgaaat ttgatcttga tgaatagata aaaaaaaccg ttcagatctg
     1621 aaaataatct gagcggtttt ttgagtaaaa atagtttatg attattctta attaaacatc
     1681 tatttatttt taaagctttc atttatcgat aagaatagtt taactgttga tttattccat
     1741 tttccgttca tctggtacga tgccttgact tagcaaccac ttgtggaaga accattctgc
     1801 cacaccgatc aatagagcag gaatcaatac tttcatccaa atatcgactg tatcattcat
     1861 aaaatataga tagagccaca agataactgc agctgaacca gcatctgcga tcgtcgctgc
     1921 gatgttccct actttacgta agatcaacag atcccctagc acatagatca tgactacaat
     1981 aattacccca acaatcgttg aatccataaa gctaacaccg tataaacctg tcagcacgac
     2041 ccacagaatc gcccagatca tgattgcctt cactaccaac gccttcacat gtttcatttt
     2101 ctgcactcct ttctcctgga taaaaaagta agattatcta acaagataac cttttcatta
     2161 ttataccatc ggctgagaga agcgagcaaa gataaggtcc tatctaaaaa tgaggaagcc
     2221 acttagaaca ttcacgtaat aatttatccg tacagacatt cgatatggtg ttcctttact
     2281 ttcgcatact gttcttttgt cagttcttta cttgtcacca gataatcgat ttcatgaaaa
     2341 gcaatggctt gatgcatgaa tttatgtgca attttttgct gtgtaactaa aacggcaagc
     2401 cggtctgatt gttttctaaa agcttttttg accattgcct ctccttcatg agtttctgtt
     2461 actccttggc tgaaatctag cccgcgacaa ctcattatcg ctaaatccgc ataatatgac
     2521 aatgcatcgt tataggcttt tgaaccgttg attgttgctc gtttcggaac aattgcccct
     2581 cccaatacag atacttttgc cgttgtatat tcactcaaca tagcagctgt gacgatgcca
     2641 ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc tgactaagtg taaagaggtt
     2701 gtactggaat ccaagaataa gaccatatca tctcgaatca aagaagaagc tgtatctgca
     2761 atcttacgtt tttctgtttc ttcttctaat ttggtgatct tcatcaggta aggcacgtca
     2821 atttttcgat tgtcaataag catggcgcca ccgcgaactc tacggatcaa tccacttttt
     2881 tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat aatttactaa ctcgatcaaa
     2941 tcggtgactc ttgcaaattg gttgtttttt agataattca caatctcctg ctgtctattc
     3001 tccacttcat tcaacataaa atcaccccat ttactttata tagtaacagg aaaaaagagg
     3061 ttgtgacaga agtgattggc ttcgagaaat aagaagaaat ttttcgaaaa aactacttct
     3121 gaacatcgtg tgtggttaag agggtatgac aagactattg tcataccctc ttgaccacac
     3181 atttttattt ttagtataaa ctagccttgc ctactgtgtt caaaatttcc attttatgtt
     3241 tgaccacttc ttgtaccttt ttattagctg ctggataaat tgcattcggc tcgtacatcg
     3301 atggattttc actcaatatt tctctcaaag cattgaaaaa gacgctttta agatctgagc
     3361 tcaaattcac tttggcaact ccgtatttta ctgcttcgct gacttctgcg tccggattac
     3421 ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg gttcaattct ttcagtaagt
     3481 cgatatttaa ttttggagtt ctgtttttcg gatacaagcc atgcgctgtt ccaattgcta
     3541 cagcaagtgt atcgattcct gtttctttca caaaaatctt cgcttgatca ggatctgtat
     3601 aaatgattgt ttcagcacct ccttcagcag aaccattatt cccaatcgtt ccaagctcag
     3661 cctccactga gacatttacc gcatgagcta acgcaaccac ttttttcgta atctgtacat
     3721 tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga agaatagccg tttcgaatcg
     3781 cccgtaagac atcttctatc gtaccgccat gatccaagtg gataacgatc ggcagtttac
     3841 ttgtataagc ataggctctt actgccgcaa caaactcatc acttaaatag gcgatttcat
     3901 caggatgaat ttctaagatt actggtgctt ctaatctctc tgcttcttcg atgattgctt
     3961 tcatcatatc atagctgcaa atattgaagg ctggtactgc aaattgattc tctttagcca
     4021 ctttcaataa ctctttcatt gtatataaca ttacgcttcc acctcatttg ttagattttc
     4081 atttttcgtc cacttgtctt gttttttctc tttcactgtt tttttcaata gtccaagcat
     4141 aaaggctgta gtgactgtac ctgatgcaat tgccaagaca tacataggta aattgttcat
     4201 aacatttgga attagcatca cccaaatccc tccatgaggt gccattgaag taactcctac
     4261 tgccatggaa atccctgcag cgactgctga acctgtcatc aaactaggta tcacgcggat
     4321 cgggtcagat accgcaaacg gtatagcacc ttctgtaata tacgaagcgc ctaaaatcca
     4381 gcatgattga cctgaagtcc gttcttcttt ggtaaattta tgtttgaata ataaagtggc
     4441 taaagcaata cctaggggag gtgtcattcc tgcagccata caagcagcaa tcggtgcatt
     4501 tactccagct gacatcaaac cgatcgagaa agtcgaagtc gatttattga tcggcccacc
     4561 catatcagct gccatcatga tcccgataag taatccgaac aatagcccgg atgtatttcc
     4621 caagctattc agccaatttg tcaaagcatc caacacccat gtgatcggtt gttcaatcac
     4681 aaataccatc aaaagcccaa taattaagat cgataaaaag gggacgacaa tcagctgata
     4741 aatagcagaa aattcttttt ttacgctaat tttatttgcc agaaaatcac agatataccc
     4801 tgctagcacg ccaccaatca ttccgccaag aaaacctgaa ccacctgtag aggccagtaa
     4861 tccggcaaca attcctgggg caaaacctgc ttttccagca atcgatgtag caatccccgc
     4921 gcctaacgca ggaaccatca tggcaaacgc tgtatctcct ccgatccttg aaaatgcagc
     4981 tgctaacaca ttatagtctg ctgagttggg atcagccgct gtaatcccaa aggcaaaact
     5041 cagtgcgatg atgattccgc ctgcgatcac aaatggaagc atgtaattca caccattcat
     5101 gaaatgatta tagattgtcg gctttttctc atttgcagct ggttcttttt cattagacac
     5161 aacgaatacg ccaatatctc tcaatgcttc gtctattact acttgcggtt ctttgattgc
     5221 tcggcttgta gagacacgct ttactttttt cccggcaaat cggtccaatt cgatttcttt
     5281 atctactgca agaatgattg catctgcctc tttgatttct tgttcagata actgattttc
     5341 tgtttttaca ccttgtgttt cgaattttgc ttgatggttt agatttcttg ctgttacttc
     5401 taatttttct gccgacatat aagtatgagc aattccagtc gggcaagcag ttacaccaac
     5461 gattttcatg attattttcc tcctttttct tcactatcga tactcggaca aagactctac
     5521 tattttcatt cttgactaat ctgtctgatg atatctgctg gcgtagtggc ttcttttatt
     5581 ccctttctaa aatctgcatt catcaatcgt ctggagagtg tgctgagaat ctctagatgc
     5641 atatctgctg atttttcagg aactacgatg gcaaaaatat attcaattgg catttcatct
     5701 atcccgctat agtccgttaa tttggctgta tgaaaaaaac acagtgctgg ttttgtcaca
     5761 tatttgcttt ttgcatgggg aatcccaaat ccatcttcta ttcctgtgga gatctcctcc
     5821 tctcgtttga atagtgcctt aacgactttt tgaacatttc ttaccttatt tgtttttttc
     5881 aacacatccc ctattcgctc aaataatgat ccttttgaat ctatcgatgg atcgaaaaca
     5941 atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa acctctcccc cacaaaaaat
     6001 gttaacgttt tcttggaact gaggtcagta tagcatcgct agctgtaccg ttcaatatta
     6061 tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata agtcattaaa atgaacgatc
     6121 tagctcacaa acaatcgcaa gtctataaca aaacaaaaag ccttccttga aaggaaagct
     6181 tagcttctaa ttttaggtaa atggtttttt acttcgaaat aacccctctt catttcgtta
     6241 atataaggtg tttttatcaa gtggataatt aaaactatat actagttttt cattgttttg
     6301 tgcattatga tcctacgact ttttcagcat caaacgaagt tttaaattgg caaaaaacat
     6361 aaaaaaacag actatcccta tcagatagtc tgctaaacgt tgagttatca gcgtttttat
     6421 tattccgcag cgtttgcgtc tttgagaccg tattttttgt tgaaacggtc cacacgtccg
     6481 tctgcttgtg tgaatttttg acgtccagtg tagaatggat gtgagtcaga agttacttcg
     6541 acacggatga ctgggtatgt attaccgtct tcccattcaa ctgtttcttg tgaagttttt
     6601 gttgaacctg acaagaattt gaaaccagtt gttgaatcca tgaatactac tgggtggtaa
     6661 tctggatgga tgttttcttt catgattttt tcgctccttt gccctgctcc atttgctgaa
     6721 ccagagttga tttgtcgatt tacaacatta acatcttaac atgtttcagt caaggttgca
     6781 attatctttt cagatttctg gtcggatttt ttttaccaaa ggagacatct tgaaattctt
     6841 tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg gatcagttgt tccgtatatt
     6901 ctaatgaatc tccagtcata ttttttcgaa gtttccaaat ttcttctaat tcttcacttg
     6961 gcatcaataa ttcttctttt cgggtacctg attttttgat atcgatggct gggaagatcc
     7021 gacgttcaga taattcacgt gaaagttgta attctagatt tccagttcct ttgaattctt
     7081 catagatcac gtcatccata cggcttcctg tatcgactaa agcggtcgct aaaatcgtca
     7141 aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa tttcttaggt ttatacaatg
     7201 cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg tggaacgact aagttatagg
     7261 cccgagcaag tctagtaata ctatccatca agataacgac atctcgttta tcttcgacta
     7321 aacgcattgc gcgttccaat actaattcag aaactcgagt atggttttgc ggctgcagat
     7381 caaatgtaga agataccacg tcgcctttca cgcttcgttc tagatccgtt acttcttccg
     7441 gtcgttcatc aatcagtaga acgattaatt ctacttccgg atagttttcg gtgataccat
     7501 ttgcgatttc ttttaataca gaagttttcc ctgcttttgg cggtgcgacg atcaatccac
     7561 gctgaccaaa accgattgga gaaaagatat cgatcattcg ggtagatagt ctttgcggtg
     7621 ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt taaggctgga aaatgcggac
     7681 gttcttttgc ttcttccgga tcttttccat taacgctttc cacgtgcatc aaaccataat
     7741 aacgttccga ttctttaggt ggacgggctt ttcctgccac tttgtcccca tttctcaaac
     7801 tgaatcgtct gatttgtgag gcagaaatat agatatcttc tgcacttggt ccataattga
     7861 ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc caatattcct tccatataat
     7921 aaaatccttg tttctctgct tgtgcacgaa tcacagctag agataattcc tttttattca
     7981 tcttactgta ataaggaatt ttaaattctt tggcataact ataaacttct tttaacgttt
     8041 tgttctctag ttctgccatt gtcaaataat cgctcattca gctcccactt cctctatcat
     8101 ttcaattgat gcccctaagg ctgttagttt ctcgataata tgatcataac cgcgtaagat
     8161 ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat agtccagcaa tcactaaaca
     8221 agcacctgcc cgaagatcag aagccacgac ttctgtgccg tgaagtttat ttggaccatt
     8281 cacgatgatc atatttcctt ccacggtaat atcagcgccc attcgtgcta gttccggcac
     8341 atggttcaca cgtttcgcat agatcgtatc gatgatttct gaagtgcctg tagtcatcaa
     8401 tagtaacgca gtaagtggct gctgcagatc tgtggcaaat cctggataag gataggtcat
     8461 gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg atttcatctt cgctgatttt
     8521 catatggaca ccaatttcct gcaattttgc tataaagctt tccaaatgtt caaagatcac
     8581 atttttcact ttgataccat ttcctactgc agctgctaat gctagatagg ttcctgcttc
     8641 gatgcggtca ggaatcatga aatgccgaca gccatgtaat gtttcgactc cttcgatacg
     8701 gatcacatct gtacccgctc ctcgaacttt tgcccccata ttattcagta aagttgccac
     8761 atctatgatt tccggttctc gtgcagcgtt ttcaattact gtctgtcctt ttgcttttac
     8821 agcagctaac atcacattga tcgttgctcc gactgaaacc acgtccatga aaatccgatt
     8881 gccacgcaat cctttatttt ctgtccgtaa atacatggca ccatgttcat ttgtcacttc
     8941 agcacctaga gcttcaaatc ctttgacgtg taggtcaatc ggtctagggc ctaaataaca
     9001 gccacctggc aggcctacta ccgcttcgcc aaatttacca agcagtgttc ccataaaata
     9061 ataagaagca cgcaaactat tgatttttcc gtgcggcatc ggaatcgata cgacttctcg
     9121 aggatcgatt tctagtatgt tgttggaaaa atggacagtt gcgcccatga tctctaaaat
     9181 ttcaattaat gaatgaacat cttgtatgtc cggcacacct tccaagatca ctggggaatc
     9241 agctaagatt gcagctggaa tcaatgcgac tgcactattt tttgcaccgc tgatcgttac
     9301 ttcaccagct aaaggtcggt ttcctttgat tacgattttt ttcatctgtt tccaccttgt
     9361 ttattaatct tttttcatgg atatgcttgt attttatcat agataaaaaa aagaaacgag
     9421 catggtctgc cgttttaagt aaaaaagaat ggagagcgac ttcaaaagtc gttctccatt
     9481 cctatatatc gtatctgtta tgtggatgct tacgctttgt tagctgaacc gaaccagtcg
     9541 atatgagatt tagcatcttc aacgattgct gctttacctg gagctaataa tttacgaggg
     9601 tcgaatcctt taccttcttt atctttacca gcttcgatat atttacgagt agctgcagca
     9661 aatgataatt ggaattcagt gttaacattt acttttgaga tacccattga gatagctttt
     9721 tcgatttgat cttgaggaat acctgatccg ccatgtaata ctaaaggcat gtctggacct
     9781 acagcatcag caattgcttg taagtggtca aaagctaatc ctttccagtt ttctggatat
     9841 tgaccatgga tgttaccgat accgcaagct aagtagtcga tacctgtagc aaccatttgt
     9901 ttgcattctt caatatcagc taattcgccg ttaccgatga ttccgtcttc ttctccgccg
     9961 attgaaccaa cttcacattc aactgagata ccttttgcat gtgctttttc aacaacgtct
    10021 ttagctaatt tcaagttttc ttcaaatggt agatgtgaac catcgaacat tactgaagta
    10081 tagcctaatt cgatacattc caaagcagct tcgtaatcac catggtctaa gtggatagca
    10141 actggaacag tgatgttcat tgcttcaact aaatcagtga ttaaatcttt acagattttg
    10201 taaccaccca tgtatttagc tgcacccatt gatgtttgga taagtactgg tgcttgtttt
    10261 gcttcagccg cttcaaggat tgcttgagtc cattccaagt tgtttgtgtt gaatccgccg
    10321 acagcgtaac cgcctttacg agcagctttt aaaaattcag ctcctgatac tactggcata
    10381 ataaaattcc tcctaagttt tgtaaaacta aatttgttaa gacaattctt aaccaacgac
    10441 cctattttac caaacatttc ggtagttttc tactaaaatc aatgaaaatg aaaattttca
    10501 agaagttttt tgcaaaaaat aaaatatcca atagcgcttt aatcgaaaaa agtcttttgc
    10561 tttctatctt ttttcctaca aataataaca aatcagaaaa agagaaaagt gacaagaaaa
    10621 ttgcttatcc ccttctcttg ctacttttct ctttctatca agttcaaaaa ttattttgtt
    10681 tcgtgatctg ctaatgatgc tccgacaaat gctttgatta atttttgcgg gcgatttgga
    10741 cgagaaatca attctgggtg gaattggcat cccacaaaga attttttctc tggcagctct
    10801 acgatttcta ctaaacggtt atctggtgag acacctgaaa agactagccc ttgttcttca
    10861 aacaattgac gatatttgtt attgaactca tagcggtgac ggtgacgttc ttgcacaaca
    10921 tcggctccat catatgcttc agccgtcttc gttccttttt tgattttaca tgggtataat
    10981 ccaaggcgta aagttccacc caaattctcg atgttttcct gatcagccat taaatcaata
    11041 atattgtttg ctgtatctgg attcgtttca gctgatgacg catcttctaa tccaacgacg
    11101 ttacgtgcaa attcgacaca agccatctgc ataccaagac aaatacctaa aaacggaacg
    11161 tcattttcgc gagcataacg aatcgcttcg attttccctt ctactccgcg atcaccaaat
    11221 ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt
    11281 aattcttgtg ctttgatcca atcgatctcg atgtctgaat tataggcgaa accagaatgt
    11341 ttcaatgctt caactactga gatatatgca tctggtaact cgacatattt accgacaagc
    11401 gcaatccgta cttttttctt caagttcaag acacgttctt ctaattcttt ccattctgtc
    11461 atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa caatttgatc cattccttgt
    11521 gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat ctggcgactc gataactgct
    11581 tctggttcga catcacaaaa ttgtgccaat ttgtttttca cgctttgaga aacaggttgc
    11641 tctgtacgta ccaccaaaat attcggttgg atacctaaac cacgtagctc ttttacactg
    11701 tgctgcgttg gtttagtttt catttctcca gcagctttca agtaaggaat caatgttgta
    11761 tggatataca taacattatc tgtaccgaca tctgctttca tttgacgaag tgcttcaagg
    11821 aatggcaaag attctatatc ccctactgtt ccccctactt ctgtaatgat cacatctgaa
    11881 tcagtcattt tagctgctcg catgattttt tctttgatct catttgtaat atgaggaata
    11941 acttgaacag ttgctcccaa gtattcgccc ttacgttctt tacgcaaaac ttcagaatag
    12001 atttttcctg tcgtcacgtt ggagtatttg ttcaaattga tgtcaataaa acgttcgtag
    12061 tggcccagat ccaaatctgt ttcagcacca tcatctgtta cgaatacttc tccgtgctga
    12121 tatgggctca tcgtccccgg atccacgttg atgtatggat caaatttttg gatagttact
    12181 tttaagccgc ggtttttcaa taagcgtcct aaagaagctg caacaatccc ttttccgatt
    12241 gatgaaacga caccgccggt cacaaaaata tattttgtca taagttcaaa aactcctttt
    12301 taatttatgt gcaggaaaag tctcagacaa taaacaaagc tccctattct tcaataagaa
    12361 tagggagccg aaatttctgt aaacttctga ccctctaaaa gggtgcccaa ggtgtataat
    12421 acaagctgac taagggaaag tcaagtactc aaatgaatac tatccgctta caattttttc
    12481 tttttcgata tcctttgaga aattatttct taagtccttt atgagtgcaa aaaaaattta
    12541 acccacataa taagcttgat ataaacatag ctaacgttaa acctaagcaa atcagaaaat
    12601 cgttactgaa aaattttaag tgaaataata gataaaaaaa taaaacaaac attatgatag
    12661 ataatatccc acctaacaaa gaactacttt ttcctacttg cagcaacagt ttatatagct
    12721 tatcgtagaa acaatctttt tctttcaccc ttatttacct cctttacgta aaaaacgtag
    12781 tccacagttc tcggattttt ggcatatctt ttctattaaa taatatcaaa aacaaaacaa
    12841 ttccactaat tttttcaaag atgacctttt tttcaacaaa agtgccttgt agggattcat
    12901 tgaaggtaac gaatcacata agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt
    12961 atttct
//
LOCUS       DACEND010000076        12913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEND010000076 DACEND010000000
VERSION     DACEND010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.078"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..634
                     /locus_tag="IW746_001802"
     CDS             200..634
                     /locus_tag="IW746_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1078701.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            735..1826
                     /locus_tag="IW746_001803"
     CDS             735..1826
                     /locus_tag="IW746_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1078702.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1856..2533
                     /locus_tag="IW746_001804"
     CDS             1856..2533
                     /locus_tag="IW746_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1078703.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2865..4160
                     /locus_tag="IW746_001805"
     CDS             2865..4160
                     /locus_tag="IW746_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078704.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4150..5610
                     /locus_tag="IW746_001806"
     CDS             4150..5610
                     /locus_tag="IW746_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1078705.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5746..7242
                     /gene="glpK"
                     /locus_tag="IW746_001807"
     CDS             5746..7242
                     /gene="glpK"
                     /locus_tag="IW746_001807"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1078706.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7256..9079
                     /gene="glpO"
                     /locus_tag="IW746_001808"
     CDS             7256..9079
                     /gene="glpO"
                     /locus_tag="IW746_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1078707.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9080..9796
                     /locus_tag="IW746_001809"
     CDS             9080..9796
                     /locus_tag="IW746_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1078708.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10102..12031
                     /locus_tag="IW746_001810"
                     /pseudo
     CDS             10102..12031
                     /locus_tag="IW746_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12157..12711
                     /locus_tag="IW746_001811"
     CDS             12157..12711
                     /locus_tag="IW746_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1078709.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4324 a         2146 c         2923 g         3520 t
ORIGIN      
        1 atactcaaaa ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt
       61 tgtcgaaaac aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag
      121 aggaggcgga cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta
      181 cgccaatgga ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt
      241 ctttagcgtg aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag
      301 gacaggtctg gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat
      361 accacaaaaa gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt
      421 tgaaaaatta gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt
      481 ttatcttaca gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact
      541 tcatcagtca tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat
      601 gaatgatgca acagataagt tacaaaaaaa ataaaatttt ttaagcaaat tagttgcatg
      661 tacaactaat cgagtggttt aatgatagat gattctcgat aggttaatca gagcgaatta
      721 tgggaggaat gacaatgtca caatttgatc aatcaattac tttaccttca ggagttcaat
      781 tgaagaaccg attgatgctg tcgcctatga cgacacaact cagctttttc aatggagtga
      841 tcaccgaaga tgagatcact tattataaac aacgttctaa aggtcttggt gctgttatta
      901 ctggtgctgc aaatgtccaa gatatcggaa aaggctggcc aggtgaactc agcatcgctc
      961 atgatgaaat gattcctcga ttaagtgagc tagcgaaagc aatccaagga gaaggcgcaa
     1021 aagcgattat tcagattttc cacggtggac gaatgacaag tcgagcaact ttatctggag
     1081 aacagcctgt atctgcaagt gcagttgcag caaaacgtcc tgatgcggaa acaccacgag
     1141 caatgagtga agaagaaatc gttgaaacaa tccaagcatt tggcaaagcc acacgtagag
     1201 cgattgaatc aggatttgat ggaatcgagt tgcatggagc aaatacgtat ttgatccaac
     1261 aatttttctc tccgcattcg aatagacgag aggatcaatg gggcggtagc ttggaaaatc
     1321 gttatcgttt tattgaagaa ttattaacaa cggttttctc agcggttgaa caatatgcta
     1381 aaaaaccatt catcgtaggg tatcgtttct caccagaaga atatgaaaca ccaggtatcc
     1441 gttttgaaga cacgatctgg cttcttgaac gattacgtga gtcaaaacta gattatctgc
     1501 atgtttcatt aaatatgtat gatcgaatag ctcgttctga taaatacaac gataaatcga
     1561 ttcttgaagt ggtccataac acgattcaag gaaagatccc tttagttggt gtcggtggga
     1621 tccgcaatcg cgaagatgta gagcaggtat tgaaatatgc agaacttgca gcaattggac
     1681 agcaaatgat cgtcgatcct gactgggcag agaaattatt ggaaaaccgt gaggatgaat
     1741 ttgtaacgaa accttttgaa gaagcatata aagagcttta tttgccaagc ccattatata
     1801 atttcttgaa agcacgttat caataacgca atttttatag aaagaggatg ggaaaatgaa
     1861 agtattgatc gtattaacaa atgtagaaaa gtacgaaaag atcgagcgtc ctacaggttt
     1921 gtggttaagt gaactgactc atttttatga tgtattagta aaaaatggga tcgatgcaga
     1981 ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg caaagtgttt tgaatatgga
     2041 tgaaatcgat tgggcctatt atgaagatga agatttcaga aatcgggcct tgggaaatac
     2101 cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg gtttattatg ctggtggcca
     2161 cggaacaatg tgggattttc cagaagcaat agatcttggg aaagtcgctt cacaaatcta
     2221 tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt gtaggattgt tgccagttac
     2281 agatgagtct ggaaaatcta ttttagctgg acgtacagtg actgggttca gtaatgaaga
     2341 agaggaagca aatggtacaa cgaatgaagt accattctta gcagaagatg ctttgaaaga
     2401 aaaaggagca aactatcagt ctggagcagc tttttcagaa gtggttcaaa ttgatgggcg
     2461 gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta ggcgaagcag ttgttgaagc
     2521 attaaagaat taaaaataaa tgatgtgtct attctaacta ttacattaca cacgctaaaa
     2581 ctcgtctttt cgttcttttc tgtttcctat ttggacagag gtacgtaaaa ggcgagtttt
     2641 tttcttttat aagagtacat tttttgaaac atctcttcag aaatgatact cttcaattag
     2701 gtccactagt acattattga gaagcagacc tagaacacgg tatgatggag agggttgatt
     2761 ctgctgcatc gctatgtatc ataaggttga aagcgagtta tgaatactgt gttttctgat
     2821 tgagaagtta gcagtagaac accttataag aaagaggaat gcagatgaac attgtaattg
     2881 tcggcgggtc gtttgcaggg attcatgcgg ctatttatct tcgccaattg atgccaacaa
     2941 gtgaaatata tttgattgaa aagcaatcga agttaggatg gattcccagc gggttcaatt
     3001 tgatattaaa aggaaaggtt accagtgaaa aacaactttc ttggatcacg aaagaagaac
     3061 tgactgaccg ctatcgaatc catgtttaca cagaaaagac agtaatagga ttgaaagaaa
     3121 aaaaagtcgt tttagcagga ggaaaaaaat tggattttga tcgtttgatt ctagcgactg
     3181 gttcaaatca aaagtttcga aatatttctg cagaatcaac gcttattcac ccagttaaaa
     3241 agatacaaaa cgtaacggct cttcagcaaa agatcatgaa tgcaaagaaa attgcaatta
     3301 ttggtgcagg acaagcagga atcgaaatag ctgaagggtt ggcatcaaga aaaaaacaaa
     3361 tccatcttta cgaaagccga aagtcaattc tttttcgtta tctagatcct gaaatgacag
     3421 aaccgttagt aaaggaaatg aaaaatcaag ggctttcact ttttttagaa gaacaagttg
     3481 tgtctttgac cgaaaatgaa accgctattg tagagacgga aaaaagaaag gagaactatg
     3541 atcttgtttt gctggctaat cattctcgtc ccgacaatca gatgtgggaa gaacaattaa
     3601 cattgaatga tgatggaacg atctgggtag atgactactt gcaaacgtct caaaaagacg
     3661 tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc aacaaatgaa aaaatgtatg
     3721 tttctctagt aaataacgct gtccgtacag cacgaaatgt cagcaagaca attagtggct
     3781 cacagacaaa ggatcaggga acctatcgtc cagtcggcaa tcagtggttt ggctatttct
     3841 taggaagcgt aggtttgaca gaagaagaga gcatttttta tccccaaaaa attgaaacag
     3901 gatacgtaga ctacaagctt acagcaataa atcaaagcaa agttaaactc aaatttctat
     3961 ttgattctga aaaaaagatt cttggagcac agctccaaag caaaaaagag atgttccacc
     4021 ttcttgacca actgacaatt gcagtagaag aaggatggac gtttgaacag ttagaagaac
     4081 atgagctatt tttccagcca gaataccgtg tgccagatat ggtgtggtca aaggtggcga
     4141 atactgccta tgaagattga agcattatta gaaagaaaag agcagttaca gatcctgatt
     4201 ttgcgaaacc ttgttctgca agggggaaca gcctctatca atggtttgag agagcatgtc
     4261 caactgtcaa aagcctcgtt tgatcaatat ttggaagata ttgaattgat tggccgaatg
     4321 atggaaaaaa aagtagaagt acagcgaaat gagtatcagg cgctgcttgt gttggacgaa
     4381 gatgtaagtt tggaaaaaat tctattgttt ttattgcagg aaagcttgaa gtttaagatg
     4441 ctcgtctatt tattagaaca tcagcaagta tccatcgttc gattagcgac agcgtttaat
     4501 atcagtgagt cttctgtttt tcgaaagatc aaagaattga accagctctt agaggaattt
     4561 ggattacaaa tcaaaaacgg tcaattatac ggagaagagc tacagatacg atatttttat
     4621 tatgaactat tccaatatat cccggaagat cagcgaccgc tttttttgca aaatacacca
     4681 gaaaaaagac cgttcattct aggattggac cgtgtattag aaacaacgtt tacggcatca
     4741 gcagaagcac agattgcttg ctggctcggg attacgaaaa aaagactatt gaatgaaaaa
     4801 agtacgtacg caactttgaa agaaaagaaa ttactctatc agtcggaccg gctttatcaa
     4861 gcaatcgatc caatcatcgt gatgcatctt agtcgaacag cagcagaact gaatgtatac
     4921 gaaacaatga tgttttacag cttttttgtt tctttttcga tagtagacga agaaatattt
     4981 tatcagtatg atttgacacg gagcaaaaag ttaccaaccg ctgttttaga tacttacatt
     5041 cgggaaacga tgttatggca ttatcgtcct cgtcgtttga aaatcaaaga agaaaaggca
     5101 gtaggctacc aaatttccca gatcaacaat gaatggtttt tctttgttgg aaagatcgaa
     5161 gtctatgagc gtgatcggct tttggaacag cagcagaaaa tgctcgggca ttccttaaca
     5221 cagttactgg cgaaattaca ggaaacagcg gtgcagcaat tgccgaggaa aagagctgaa
     5281 gatagtgagc ttagttacct gatgatccag tatgccaatg ttcttttgat gatcgatttt
     5341 tatattgcaa aaccagtagt gattggaatc gatttagaaa gtctgccgat ttatcgtatt
     5401 gcttttcaac aatatctgat cagagaactg aggggaatcg gcggtattga gattggaagt
     5461 tatgaagaag gtaaagaata tgatttggtg atcacatttt gccagagaaa caaaaaacag
     5521 tgtgaatatt atctgtcaga atttgcgtca ccttacgaca ttattcgatt gaaaagaaga
     5581 atagagacgc tgaaaaaaga aaagaattga ttaaaaaata gcttcataat aaaaaactgt
     5641 caaaaaaaga aaaaaagaat aaaattttga cagtttcttt ttttaaaaca gtagtatgat
     5701 aagagtagtt aaagaaagcg tttgcatata atggagggaa tcacaatgac agaatcaacg
     5761 tatatcatgg cgattgacca aggtacaact agctcacgag caattatctt tgacaagaaa
     5821 ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat atttcccgca agaaagttgg
     5881 gtggaacatg atgcaaatga aatctggaat tctgtacagt cagttattgc cggagcgttc
     5941 atcgaatcag ggatcaaacc aaatcagatt gctgggattg gcataacgaa ccagcgtgaa
     6001 acaacagtaa tatgggaaaa agatacagga cgtccaatct atcatgcagt tgtttggcaa
     6061 tccagacagt cagctggaat tgcagataac ttaaaaaatg aaggctatca agaatttttc
     6121 cacgaaaaaa cagggttagt gatcgatgcc tatttctcag caacgaagat acgctggatt
     6181 cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag gcgaattgat gtttggaacg
     6241 atcgattctt ggttagtatg gaaactgacc gatggacaag cacatgttac tgattactcg
     6301 aatgccagtc ggacgatgtt gttcaatatc catcagctag attgggatca ggagatttta
     6361 gatttactaa atattcctcg agtgatgtta ccaaaagtca cttctaactc agaagtttat
     6421 ggttatacgc aaggttatca tttttatgga agcgaagcac caatttcagg gatggcagga
     6481 gaccagcaag ccgcattatt cggacaaatg gcattcgaac caggcatggt caagaatact
     6541 tatggtacag gttcatttat tgtcatgaat accggtgaaa aaccgcaatt atccaaaaat
     6601 aatttattga cgacaattgg ttacggcata aatggaaagg tttactatgc tttagaagga
     6661 agtatttttg tggctggttc ttcgattcaa tggctgagag atggacttca aatgttgcaa
     6721 aaagctagcg attcagaaga cgcagcgaaa cgctctacaa gcgaagatga ggtttatgtt
     6781 gttcctgcat tcgttgggct aggtgctcct tattgggatc aggcagcacg aggggctatg
     6841 tttggcttga cacgtggtac gacaaaagaa gatatcatta aagctacttt acagtcgatt
     6901 gcttatcaag tgagagatat catcgatacg atgcaagatg acactggaat caagattcct
     6961 gtattgaaag tagatggcgg tgcagcaaac aatgaatatc tgatgcaatt ccaaacagat
     7021 atcttgaatg taccgatcca acgagcagaa aacttggaaa caacggcgct aggggcagca
     7081 tttttagcag gattagctgt cggttattgg aaagatacag acgagatccg cgaattttat
     7141 gaagctggca agctatttga agttcaaatg gaggaagagc gccgagaaaa actttacaac
     7201 ggttggaaaa aagcagtaaa agctacccaa gcatttgaat aaggaggaaa tccatatgtt
     7261 ttcaaacaag acaagacaag atagcattca aaaaatgcag caagaagaat tggatctgtt
     7321 gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc cagtcagcag catcaggaat
     7381 caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg acttcctctc gctcgaccaa
     7441 acttgttcat ggcggtattc gttatctgaa gacatttgat gtggaagtag tagctgacac
     7501 agttggtgaa cgtgcagtcg tacaaagtat tgccccacac atcccaaaac cagatccaat
     7561 gcttttgcca atctatgagg gcgaaggagc aacaaccttc aatatgttct cggtcaaagt
     7621 agcaatggac ctttatgaca aactggcaaa tgtgacagga accaaatatg agaattatac
     7681 gctgacacca gaagaagtat tggaaagaga accgtttttg aaaaaagaag ggctaaaagg
     7741 cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt ttagtgattg ataatatcaa
     7801 aaaggctgcc gaagatgggg cttatctagt aagtaaaatg aaagcggttg gctttttata
     7861 tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg ctgacagatg aagtgatcga
     7921 aatcaaagca aaattagtga tcaatacgag tggtccttgg gtagataaag tacgaaactt
     7981 gaattttacg cgtccagtct ctcctaaaat gcgtccaact aaagggatcc atttagttgt
     8041 agatgcgaaa aaactgcctg taccgcaacc cacatacttc gatacaggaa agcaagatgg
     8101 gcggatggtt tttgctattc caagagaaaa caagacttac tttggtacga cagatacgga
     8161 ttaccaagga gattttacgg atccgaaagt cacacaagaa gatgtagatt atctattgga
     8221 tgtgatcaat catcgctatc cagaagcaaa tatcacattg aatgatatcg aagcaagctg
     8281 ggcaggactt cgtccactgt tgattggtaa ttctggttct gattacaatg gtggagataa
     8341 tggatcgatt tcagacaaga gcttcaataa agttgtcgat acagtaagtg aatataagga
     8401 aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac catttggaaa acagccgtga
     8461 tgaaaaagca ccttctacga tttccagagg tagttcttta gaaagagaac cagatggttt
     8521 gctgacttta tctggtggga aaatcaccga ttaccgtaag atggcagaag gagctttgcg
     8581 attgatacgc caattattaa aagaagaata cggaatagag acgaaagaaa tcgactctaa
     8641 aaaatatcag atttcaggtg gaaacttcga tccaactaaa ttagaagaaa cagtgacaga
     8701 attagcaaaa gaaggagtag cagccggttt agaggaagaa gatgctactt atatcgctga
     8761 tttttacggg actaatgccc gacgtatctt tgaattagca aaagaaatgg caccttatcc
     8821 tggtttgagt ctcgctgagt cagctcggtt acgttatggt ttagaagaag aaatggtttt
     8881 ggccccaggc gattatctca ttcgtcgtac aaatcatctg ttgtttgaac gagatcagct
     8941 tgatgagatc aagcaacctg tgatcgatgc aattgctgga tattttgaat ggacagaaga
     9001 ggagaaggcg caacagacta aacgtttaga agcattgatt gcagaatcag atctgcggga
     9061 actaaagggg gagaaataaa tgaacagtga tatgactcaa atcatggggg agtttattgg
     9121 gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg gctgtgaatt taaacaaaag
     9181 taaagcacag gcttctggct ggattgtcat cgcatttggt tggggactcg ctgtaacaat
     9241 ggctgtctat atttctggat tcatgggccc agctcatttg aatcctgcag tctctctagc
     9301 tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt cctttcatca ttgcgcaagt
     9361 tttaggagct tttgcagggg ccattcttgt ttggctttct tatttgccac attggaatgc
     9421 aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca ggtcctgcta tacggaatta
     9481 tccagcaaat gtgatcacag aattaattgg tacatttgta ttagttcttg ggttgctagc
     9541 ttttggacaa aacgaatttg cacctggaac gaacgtattc gctgtaggtg gattgatctt
     9601 agccatcggt ctttcattag gagggccaac aggatatgca atcaaccctg ctcgggactt
     9661 tggtcctcga ttagcacatg cggtattgcc aatagctaat aaaggaactt cagattgggc
     9721 atattcatgg gtaccaatcg caggtccaat gatcggagca attatcgcgg taggtgtcta
     9781 ttcattaatg gtgtaaatct ataaaacata ctaaagtttg acaaccaaaa tgtgtcttgc
     9841 tatagttaag tggaaaataa ataagaccta gaagaatggg gtatgttttg tgagtgtagc
     9901 tggtatctaa tcagtaagtc gaatagccaa taaacaaagt ttatttggtg tgcttatgaa
     9961 acggtacctg tcaggaaaac aatatctttg tattctaata aggataggac atgacttttt
    10021 taagctgtgt tctatccttt tatttatctc taggccctta tttattttgg gacaggtatc
    10081 tcttcaagaa aaggagagaa tatggacaac gaaatgatta gtcaattaca atttgatcgg
    10141 atcaaaaagg aaatccaagc aagagcaatt ggaaactata gcaaaaaacg aatcagcgat
    10201 atgacggtct cgacaaacct acaaactgtc ttggatcgtc aagaagaaac aagagaagca
    10261 cgcttgatct tagaaagcag tcagcatgtt ccatttatgg ggttgccgag gatcgatgca
    10321 ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa ccgatttgat cgaatatgcg
    10381 gattttctta gaagcagtcg gatgatcaca aagttttttg ataagaatca ataccagaca
    10441 cccttgttgt ttgcttatag caaacatctt cctgatttga taaatgtaga agagttgatt
    10501 gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt cacgtaattt gcgtaaagta
    10561 cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa aattactgaa attcttgcgt
    10621 catccgaaaa acaaagaaat gatccaagaa gcaatgattg ttcaaaaagg cgaatactat
    10681 acgattccta tcaaagccag ctataaaaat aaaatagatg gaacgattat cgatgaatca
    10741 aataaaagaa cgacggtttt tattgaacca actgtgattt ctaaattgaa tgaacactat
    10801 cagttgttaa aagctgaaga aatcagtgaa gaatatcaag tcctggcggc attgactggt
    10861 gctatcgcag aaaacgaaga agtgatcgat ttattgatcg aaacgatcac ggtgttagat
    10921 atcatctttg ctcgggcaaa atttagccga gaaatcaatg gaagcacacc tagaatcaat
    10981 aaatcggaac atatcattat caagcaaggc agacatccat ttttacctga gcatgcggta
    11041 ccattggatg ttgaaatcgg taaagactat cgaggcttga ttatcaccgg agcaaatgcc
    11101 ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga ctttgatggc gatgttcggt
    11161 atgcaagttc cggcaaaaga agggacagag cttgctgtct ttgatgagat tttcgtcgat
    11221 gtcggtgacc accaaaattt agaaaatgcg ttgagtacat tttctggtca tatgcaaaac
    11281 atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg tacttttaga tgagattggc
    11341 agtggaacag agccaaatga aggggctgca ctagcgatcg caatcatgga atctatgtat
    11401 gagcaagggg cattgatcat tgccacgacc cattatggag aaatcaagaa attcgccgga
    11461 gatcatgaag attttgtgcc ggctgctatg gctttcgatc gggaagcact tcgtcctaaa
    11521 tatcagttgc gggttggaag aaacaggaga aagccaagct ttgtggattg ctcataaaat
    11581 ggatatgtcg atgaaattga tccatcaagc agagcgttac ttggcggaaa aagcctatcg
    11641 gacacagaaa aaagaatttc ttcctaagac agcagttaca aataggaagg agaataaaaa
    11701 agaacgtcag ctctttgcaa aaggtgatcg gatatttgtc aatgaatacc aaaaagaagc
    11761 gttagtttat gaagatatag gtgaagatac gattgatgta tatttagaca aaaaaataat
    11821 ccacgtgcct cgtcagcgtg ttcgtctggt gcgttcagca gaagatctgt acccgacagg
    11881 ttacgattta gacagtctgt ttatcgatta taaaacacgt aaacggcagc gtgacctaga
    11941 gcgaggatca aaaaaagctc ataaagtact agtaaaagaa atgcgcaaac gacaggaaga
    12001 aagaagagta aacgatgaaa acagcaagtg attatctcaa attagctcat aatatcagtg
    12061 tctgtaaaga aaaataaaaa cagtgaaatc attggtttta ttccccgtgt aagctaaaat
    12121 aaaaagaaaa gagtgctgag gaggtagggc gataaaatga aattaagcac agactggcgt
    12181 aaagaaatac aaacgattcc gaatctttta tcgatattcc gtattttttt gctgccgatt
    12241 tatctttatt ttgtgctgag acagtcattt tatatagcag gagcggtcat tgttgtctca
    12301 ggtttgtctg attatctaga cggggtcatt gctcggagat ataatcaggt aacagactta
    12361 gggaaggtgc ttgatccgtt tgcagataaa ttgacacaac tttttctgat tttgtctatg
    12421 gcatggtatc gaccgtggct ttggctgttg tttggtctat tcttgataaa agaaggcttt
    12481 atgtttgtag ctggattaat cgggctatct aaaaatatca aattaagtgg agcgaagtgg
    12541 tatggcaaag tagcgactgc tgtcatctat gttggcatga tccttttgct gcttttccca
    12601 gaattaccaa cgctgtgggt gagagtcatt tttgccgtca tcacttacgg tcttttacaa
    12661 tcttttgtct tgtatgctgt cgagtaccga aagatgtttc aaagaaaata aaaaaggggt
    12721 gtgacctatg tcgcattcct ttttttgata aatagactcc cagtagctac ttctcgataa
    12781 taagccaacc gaaccaaaaa caggagaagt tgtttttgag attatttctt gtgattcgga
    12841 gttaaatggt aggctcacaa cctcttggta aacggtgttc aatcagctga ccttcggtat
    12901 tagctcaaaa aac
//
LOCUS       DACEND010000077        12740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEND010000077 DACEND010000000
VERSION     DACEND010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.079"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..142)
                     /locus_tag="IW746_001812"
     CDS             complement(<1..142)
                     /locus_tag="IW746_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1078710.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDP"
     gene            complement(144..341)
                     /locus_tag="IW746_001813"
     CDS             complement(144..341)
                     /locus_tag="IW746_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1078711.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(735..2462)
                     /locus_tag="IW746_001814"
     CDS             complement(735..2462)
                     /locus_tag="IW746_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1078712.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2455..3648)
                     /locus_tag="IW746_001815"
     CDS             complement(2455..3648)
                     /locus_tag="IW746_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1078713.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3835..4479)
                     /locus_tag="IW746_001816"
     CDS             complement(3835..4479)
                     /locus_tag="IW746_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078714.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4573..4707)
                     /locus_tag="IW746_001817"
     CDS             complement(4573..4707)
                     /locus_tag="IW746_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1078715.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4709..6841)
                     /gene="feoB"
                     /locus_tag="IW746_001818"
     CDS             complement(4709..6841)
                     /gene="feoB"
                     /locus_tag="IW746_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1078716.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6838..7311)
                     /locus_tag="IW746_001819"
     CDS             complement(6838..7311)
                     /locus_tag="IW746_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1078717.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7712..7936
                     /gene="nrdH"
                     /locus_tag="IW746_001820"
     CDS             7712..7936
                     /gene="nrdH"
                     /locus_tag="IW746_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1078718.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8095..10254
                     /gene="nrdE"
                     /locus_tag="IW746_001821"
     CDS             8095..10254
                     /gene="nrdE"
                     /locus_tag="IW746_001821"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1078719.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10304..11269
                     /gene="nrdF"
                     /locus_tag="IW746_001822"
     CDS             10304..11269
                     /gene="nrdF"
                     /locus_tag="IW746_001822"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1078720.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11358..12497)
                     /locus_tag="IW746_001823"
     CDS             complement(11358..12497)
                     /locus_tag="IW746_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1078721.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         3928 a         2617 c         2204 g         3991 t
ORIGIN      
        1 tgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
       61 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      121 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      181 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      241 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      301 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      361 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      421 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      481 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      541 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      601 gtctttgaac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      661 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      721 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      781 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      841 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
      901 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
      961 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1021 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1081 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1141 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1201 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1261 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1321 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1381 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1441 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1501 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1561 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1621 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1681 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1741 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1801 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1861 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     1921 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     1981 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2041 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2101 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2161 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2221 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2281 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2341 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2401 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2461 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2521 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2581 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2641 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2701 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2761 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2821 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     2881 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     2941 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3001 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3061 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3121 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3181 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3241 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3301 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3361 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3421 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3481 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3541 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3601 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3661 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3721 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3781 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3841 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     3901 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     3961 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4021 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4081 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4141 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4201 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4261 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4321 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4381 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4441 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4501 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4561 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4621 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4681 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4741 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4801 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4861 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     4921 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     4981 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5041 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5101 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5161 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5221 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5281 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5341 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5401 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5461 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5521 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5581 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5641 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5701 gtcgatccat gaaaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5761 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5821 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     5881 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     5941 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6001 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6061 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6121 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6181 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6241 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6301 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6361 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6421 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6481 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6541 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6601 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6661 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6721 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6781 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6841 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     6901 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     6961 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7021 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7081 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7141 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7201 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7261 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7321 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7381 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7441 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7501 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7561 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7621 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7681 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7741 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7801 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7861 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     7921 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     7981 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8041 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8101 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8161 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8221 gtcaaaccaa atacgatgac ttttccatct attatggaaa aaatcaatta cttgatcgat
     8281 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8341 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8401 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8461 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8521 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8581 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8641 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8701 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8761 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8821 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     8881 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     8941 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9001 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9061 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9121 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9181 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9241 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9301 ttaggaacag atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9361 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9421 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9481 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9541 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9601 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9661 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9721 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9781 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9841 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
     9901 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
     9961 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10021 gcaacggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10081 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10141 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10201 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10261 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10321 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10381 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10441 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10501 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10561 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10621 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10681 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10741 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10801 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10861 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    10921 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    10981 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11041 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11101 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11161 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11221 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11281 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11341 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11401 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11461 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11521 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11581 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11641 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11701 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11761 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11821 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    11881 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    11941 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12001 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12061 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12121 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12181 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12241 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12301 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12361 aaaaaaattg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12421 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12481 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12541 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12601 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12661 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12721 gccaaacgaa aaatataagg
//
LOCUS       DACEND010000078        12727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEND010000078 DACEND010000000
VERSION     DACEND010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.080"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..802)
                     /locus_tag="IW746_001824"
     CDS             complement(83..802)
                     /locus_tag="IW746_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1078722.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(851..2497)
                     /locus_tag="IW746_001825"
     CDS             complement(851..2497)
                     /locus_tag="IW746_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1078723.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2676..3266
                     /locus_tag="IW746_001826"
     CDS             2676..3266
                     /locus_tag="IW746_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1078724.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3358..4863
                     /locus_tag="IW746_001827"
     CDS             3358..4863
                     /locus_tag="IW746_001827"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1078725.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW746_001828"
     CDS             complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW746_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1078726.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6299..6817)
                     /locus_tag="IW746_001829"
     CDS             complement(6299..6817)
                     /locus_tag="IW746_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078727.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6833..7159)
                     /locus_tag="IW746_001830"
     CDS             complement(6833..7159)
                     /locus_tag="IW746_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1078728.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7172..9151)
                     /locus_tag="IW746_001831"
     CDS             complement(7172..9151)
                     /locus_tag="IW746_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1078729.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9172..9486)
                     /locus_tag="IW746_001832"
     CDS             complement(9172..9486)
                     /locus_tag="IW746_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1078730.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9654..9947
                     /locus_tag="IW746_001833"
     CDS             9654..9947
                     /locus_tag="IW746_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1078731.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10025..11137)
                     /locus_tag="IW746_001834"
     CDS             complement(10025..11137)
                     /locus_tag="IW746_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1078732.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11338..11514
                     /locus_tag="IW746_001835"
     CDS             11338..11514
                     /locus_tag="IW746_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078733.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            11524..11703
                     /locus_tag="IW746_001836"
     CDS             11524..11703
                     /locus_tag="IW746_001836"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078734.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            11897..12067
                     /locus_tag="IW746_001837"
     CDS             11897..12067
                     /locus_tag="IW746_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1078735.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12491..>12727)
                     /locus_tag="IW746_001838"
     CDS             complement(12491..>12727)
                     /locus_tag="IW746_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1078736.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4020 a         2555 c         2152 g         4000 t
ORIGIN      
        1 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
       61 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      121 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      181 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      241 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      301 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      361 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
      421 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
      481 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
      541 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
      601 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
      661 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
      721 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
      781 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
      841 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
      901 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
      961 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1021 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1081 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1141 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1201 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1261 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1321 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1381 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     1441 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     1501 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     1561 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     1621 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     1681 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     1741 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     1801 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     1861 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     1921 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     1981 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
     2041 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2101 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2161 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2221 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2281 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2341 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     2401 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     2461 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
     2521 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     2581 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     2641 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     2701 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     2761 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     2821 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     2881 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     2941 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3001 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3061 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3121 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3181 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3241 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3301 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3361 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     3421 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     3481 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     3541 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     3601 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     3661 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     3721 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     3781 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     3841 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     3901 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     3961 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4021 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4081 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4141 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4201 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4261 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4321 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4381 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     4441 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
     4501 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     4561 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     4621 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     4681 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     4741 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     4801 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     4861 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     4921 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     4981 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5041 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5101 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5161 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5221 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5281 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5341 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     5401 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     5461 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     5521 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     5581 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     5641 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     5701 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     5761 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     5821 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     5881 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     5941 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6001 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6061 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6121 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6181 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6241 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6301 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6361 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     6421 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     6481 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     6541 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     6601 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     6661 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     6721 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     6781 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     6841 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     6901 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     6961 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7021 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7081 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7141 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7201 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7261 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7321 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7381 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     7441 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     7501 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     7561 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     7621 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     7681 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     7741 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     7801 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     7861 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     7921 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     7981 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8041 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8101 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8161 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8221 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8281 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8341 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     8401 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     8461 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     8521 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     8581 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     8641 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     8701 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     8761 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     8821 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     8881 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     8941 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9001 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9061 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9121 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9181 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9241 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9301 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9361 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     9421 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     9481 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     9541 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     9601 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     9661 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     9721 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     9781 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     9841 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     9901 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     9961 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10021 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10081 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10141 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10201 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10261 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10321 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10381 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    10441 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    10501 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    10561 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    10621 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    10681 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    10741 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    10801 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    10861 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    10921 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    10981 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11041 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11101 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11161 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11221 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11281 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11341 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    11401 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    11461 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    11521 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    11581 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    11641 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    11701 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    11761 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    11821 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    11881 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    11941 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12001 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12061 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12121 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12181 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12241 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12301 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12361 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    12421 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    12481 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    12541 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    12601 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    12661 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    12721 tcgcatc
//
LOCUS       DACEND010000079        12464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEND010000079 DACEND010000000
VERSION     DACEND010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.081"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            333..1961
                     /gene="treC"
                     /locus_tag="IW746_001839"
     CDS             333..1961
                     /gene="treC"
                     /locus_tag="IW746_001839"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1078737.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(2021..3202)
                     /locus_tag="IW746_001840"
     CDS             complement(2021..3202)
                     /locus_tag="IW746_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1078738.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3454..4977
                     /locus_tag="IW746_001841"
     CDS             3454..4977
                     /locus_tag="IW746_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1078739.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(5046..5774)
                     /locus_tag="IW746_001842"
     CDS             complement(5046..5774)
                     /locus_tag="IW746_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078740.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKFFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5787..7067)
                     /locus_tag="IW746_001843"
     CDS             complement(5787..7067)
                     /locus_tag="IW746_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1078741.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(7208..7810)
                     /locus_tag="IW746_001844"
     CDS             complement(7208..7810)
                     /locus_tag="IW746_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1078742.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            8055..8261
                     /locus_tag="IW746_001845"
     CDS             8055..8261
                     /locus_tag="IW746_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078743.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8573..9292
                     /locus_tag="IW746_001846"
     CDS             8573..9292
                     /locus_tag="IW746_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1078744.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9373..10056)
                     /locus_tag="IW746_001847"
     CDS             complement(9373..10056)
                     /locus_tag="IW746_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1078745.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            10274..12460
                     /locus_tag="IW746_001848"
     CDS             10274..12460
                     /locus_tag="IW746_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1078746.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4508 a         2134 c         2391 g         3431 t
ORIGIN      
        1 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
       61 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
      121 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
      181 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
      241 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
      301 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
      361 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
      421 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
      481 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
      541 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
      601 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
      661 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
      721 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
      781 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
      841 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
      901 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
      961 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
     1021 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
     1081 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
     1141 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
     1201 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
     1261 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
     1321 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
     1381 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
     1441 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
     1501 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
     1561 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
     1621 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
     1681 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
     1741 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
     1801 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
     1861 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
     1921 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
     1981 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
     2041 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
     2101 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
     2161 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
     2221 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
     2281 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
     2341 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
     2401 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
     2461 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
     2521 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
     2581 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
     2641 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
     2701 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
     2761 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
     2821 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
     2881 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
     2941 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
     3001 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
     3061 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
     3121 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
     3181 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
     3241 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
     3301 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
     3361 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
     3421 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
     3481 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
     3541 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
     3601 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
     3661 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
     3721 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
     3781 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
     3841 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
     3901 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
     3961 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
     4021 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
     4081 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
     4141 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
     4201 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
     4261 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
     4321 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
     4381 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
     4441 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
     4501 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
     4561 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
     4621 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
     4681 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
     4741 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
     4801 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
     4861 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
     4921 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
     4981 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
     5041 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
     5101 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
     5161 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
     5221 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
     5281 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
     5341 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaaattt gctcaggata
     5401 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
     5461 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
     5521 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
     5581 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
     5641 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
     5701 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
     5761 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
     5821 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
     5881 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
     5941 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
     6001 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
     6061 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
     6121 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
     6181 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
     6241 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
     6301 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
     6361 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
     6421 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
     6481 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
     6541 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
     6601 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
     6661 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
     6721 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
     6781 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
     6841 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
     6901 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
     6961 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
     7021 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
     7081 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
     7141 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
     7201 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
     7261 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
     7321 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
     7381 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
     7441 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
     7501 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
     7561 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
     7621 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
     7681 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
     7741 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
     7801 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
     7861 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
     7921 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
     7981 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt gtaaaaggtg
     8041 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
     8101 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
     8161 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
     8221 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
     8281 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
     8341 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
     8401 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
     8461 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
     8521 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
     8581 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
     8641 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
     8701 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
     8761 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
     8821 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
     8881 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
     8941 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
     9001 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
     9061 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
     9121 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
     9181 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
     9241 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
     9301 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
     9361 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
     9421 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
     9481 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
     9541 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
     9601 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
     9661 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
     9721 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
     9781 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
     9841 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
     9901 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
     9961 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    10021 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    10081 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    10141 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    10201 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    10261 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    10321 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    10381 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    10441 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    10501 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    10561 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    10621 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    10681 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    10741 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    10801 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    10861 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    10921 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    10981 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    11041 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    11101 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    11161 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    11221 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    11281 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    11341 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    11401 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    11461 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    11521 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    11581 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    11641 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    11701 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    11761 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    11821 cgatcaatac ttttctccta aatatcaaaa tatatccgtt gcagattata ttttgtcgat
    11881 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    11941 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    12001 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    12061 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    12121 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    12181 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    12241 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    12301 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    12361 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    12421 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga taga
//
LOCUS       DACEND010000080        12174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEND010000080 DACEND010000000
VERSION     DACEND010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.082"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            595..1923
                     /locus_tag="IW746_001849"
     CDS             595..1923
                     /locus_tag="IW746_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1078747.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="IW746_001850"
     CDS             2125..2958
                     /locus_tag="IW746_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1078748.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..3711
                     /locus_tag="IW746_001851"
     CDS             2974..3711
                     /locus_tag="IW746_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1078749.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3866..4834)
                     /locus_tag="IW746_001852"
     CDS             complement(3866..4834)
                     /locus_tag="IW746_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1078750.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5066..5899
                     /locus_tag="IW746_001853"
     CDS             5066..5899
                     /locus_tag="IW746_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1078751.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5933..6772
                     /locus_tag="IW746_001854"
     CDS             5933..6772
                     /locus_tag="IW746_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1078752.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6798..7358
                     /locus_tag="IW746_001855"
     CDS             6798..7358
                     /locus_tag="IW746_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1078753.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7557..9278
                     /locus_tag="IW746_001856"
     CDS             7557..9278
                     /locus_tag="IW746_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1078754.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9443..10585
                     /locus_tag="IW746_001857"
     CDS             9443..10585
                     /locus_tag="IW746_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1078755.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10601..11812
                     /gene="thiI"
                     /locus_tag="IW746_001858"
     CDS             10601..11812
                     /gene="thiI"
                     /locus_tag="IW746_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1078756.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4150 a         2041 c         2527 g         3456 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     3721 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     3781 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     3841 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     3901 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     3961 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     4021 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     4081 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     4141 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     4201 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     4261 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     4321 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     4381 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     4441 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     4501 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     4561 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     4621 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     4681 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     4741 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     4801 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     4861 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     4921 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     4981 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     5041 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     5101 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     5161 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     5221 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     5281 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     5341 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     5401 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     5461 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     5521 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     5581 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     5641 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     5701 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     5761 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     5821 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     5881 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     5941 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     6001 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     6061 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     6121 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     6181 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     6241 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     6301 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     6361 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     6421 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     6481 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     6541 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     6601 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     6661 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     6721 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     6781 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     6841 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     6901 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     6961 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     7021 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     7081 cagatatcct ttactttact tatggttctt tttcattact aggaatagtg cctgatcgaa
     7141 tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga aaacctcatt
     7201 atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa tatgctccag
     7261 aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct aagaagaaat
     7321 ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac tatttttgtt
     7381 atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc atgaaaatag
     7441 tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg gatttatgcc
     7501 aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa ccataaatgg
     7561 gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt gtcgtctttt
     7621 tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca
     7681 aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc
     7741 tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat gacatatcta
     7801 cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt
     7861 ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg gaccaaatgg
     7921 agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca
     7981 actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa aaagtcagag
     8041 aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc aaagtgatcg
     8101 aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt gaagcccgcg
     8161 aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg aaaaaaattc
     8221 caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa atcagtgata
     8281 cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt gctgaagata
     8341 tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa acagaagttg
     8401 aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta tatgatatct
     8461 tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg attgcagagt
     8521 atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat cacacatctc
     8581 aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa accgaagtgg
     8641 aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac gaaattcctt
     8701 attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat atcgaaaagc
     8761 aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag
     8821 aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac
     8881 gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact gaccgtattg
     8941 aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc aatcgtttag
     9001 ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat ttagtagatg
     9061 cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat acacacgaag
     9121 aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat cactatcaag
     9181 aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct ttcaaacgca
     9241 tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt agtaaaccag
     9301 caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg aaaattgaaa
     9361 caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa tagaaaagaa
     9421 tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa ccccaatctt
     9481 tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga atccatcaag
     9541 cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa agcagatcgc
     9601 tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa cagaaggaga
     9661 taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc atatgatcat
     9721 ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag aattaggaat
     9781 cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg aattgaaaga
     9841 attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg aagtaggatc
     9901 gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa ttcatttcca
     9961 tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga cagaaagagt
    10021 tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg gtttcattta
    10081 ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag aaagaaatca
    10141 gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt tacgtttatc
    10201 attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag ctctaatcaa
    10261 tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact atgcaccgca
    10321 tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga
    10381 aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata tggcaagcag
    10441 tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc gaatcagctt
    10501 agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca agcagcttta
    10561 taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca gtgaaattat
    10621 ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta tcatgcaact
    10681 agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg
    10741 ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc caaaactagc
    10801 aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag atgtcgaagt
    10861 tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag aaacatttaa
    10921 aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat tgaatcaaac
    10981 attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc gtcaaccaga
    11041 tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa
    11101 gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc tttctggggg
    11161 gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt
    11221 tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa aagatctaac
    11281 agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat ttaccgagat
    11341 tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta ctcgtcgaat
    11401 gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca ttttgaatgg
    11461 tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta tcaatgaagt
    11521 aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa ttattgaagt
    11581 agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt gctgtacgat
    11641 ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc aatatgaaga
    11701 gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga tggaagagat
    11761 acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat aagaggttat
    11821 gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc tccatgtatt
    11881 ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat caagaagtta
    11941 tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga gatcaagcta
    12001 cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag
    12061 tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa
    12121 tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag gttg
//
LOCUS       DACEND010000081        12074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEND010000081 DACEND010000000
VERSION     DACEND010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.083"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..1440)
                     /locus_tag="IW746_001859"
     CDS             complement(133..1440)
                     /locus_tag="IW746_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1078757.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="IW746_001860"
     CDS             complement(1443..2024)
                     /locus_tag="IW746_001860"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1078758.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="IW746_001861"
     CDS             complement(2596..3888)
                     /locus_tag="IW746_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1078759.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="IW746_001862"
     CDS             complement(3885..4508)
                     /locus_tag="IW746_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078760.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="IW746_001863"
     CDS             complement(4511..5347)
                     /locus_tag="IW746_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1078761.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="IW746_001864"
     CDS             complement(5364..6251)
                     /locus_tag="IW746_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1078762.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="IW746_001865"
     CDS             complement(6267..7550)
                     /locus_tag="IW746_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1078763.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="IW746_001866"
     CDS             complement(8233..8916)
                     /locus_tag="IW746_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1078764.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="IW746_001867"
     CDS             complement(8934..9551)
                     /locus_tag="IW746_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1078765.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="IW746_001868"
     CDS             complement(9561..10385)
                     /locus_tag="IW746_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1078766.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="IW746_001869"
     CDS             complement(10378..10938)
                     /locus_tag="IW746_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078767.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="IW746_001870"
     CDS             11112..12068
                     /locus_tag="IW746_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1078768.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2653 c         2047 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DACEND010000082        12069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEND010000082 DACEND010000000
VERSION     DACEND010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.084"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..488)
                     /locus_tag="IW746_001871"
     CDS             complement(147..488)
                     /locus_tag="IW746_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1078769.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="IW746_001872"
     CDS             complement(556..2727)
                     /locus_tag="IW746_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1078770.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="IW746_001873"
     CDS             2938..3795
                     /locus_tag="IW746_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1078771.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="IW746_001874"
     CDS             complement(3866..4723)
                     /locus_tag="IW746_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1078772.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="IW746_001875"
     CDS             complement(4708..7410)
                     /locus_tag="IW746_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1078773.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="IW746_001876"
     CDS             complement(7423..8712)
                     /locus_tag="IW746_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1078774.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="IW746_001877"
     CDS             8862..9908
                     /locus_tag="IW746_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078775.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="IW746_001878"
     CDS             9910..12063
                     /locus_tag="IW746_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1078776.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3320 a         2553 c         2202 g         3994 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggctcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taacaagag
//
LOCUS       DACEND010000083        11988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEND010000083 DACEND010000000
VERSION     DACEND010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.085"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..453)
                     /locus_tag="IW746_001879"
     CDS             complement(91..453)
                     /locus_tag="IW746_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1078777.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(464..1585)
                     /locus_tag="IW746_001880"
     CDS             complement(464..1585)
                     /locus_tag="IW746_001880"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1078778.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1601..1951)
                     /locus_tag="IW746_001881"
     CDS             complement(1601..1951)
                     /locus_tag="IW746_001881"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1078779.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2081..3592)
                     /locus_tag="IW746_001882"
     CDS             complement(2081..3592)
                     /locus_tag="IW746_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1078780.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3914..5266)
                     /locus_tag="IW746_001883"
     CDS             complement(3914..5266)
                     /locus_tag="IW746_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1078781.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5419..6495)
                     /locus_tag="IW746_001884"
     CDS             complement(5419..6495)
                     /locus_tag="IW746_001884"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1078782.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6691..8436
                     /locus_tag="IW746_001885"
     CDS             6691..8436
                     /locus_tag="IW746_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1078783.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8716..9153)
                     /locus_tag="IW746_001886"
     CDS             complement(8716..9153)
                     /locus_tag="IW746_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1078784.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9137..10510)
                     /locus_tag="IW746_001887"
     CDS             complement(9137..10510)
                     /locus_tag="IW746_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078785.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10832..11320)
                     /locus_tag="IW746_001888"
     CDS             complement(10832..11320)
                     /locus_tag="IW746_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1078786.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11342..11944)
                     /locus_tag="IW746_001889"
     CDS             complement(11342..11944)
                     /locus_tag="IW746_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1078787.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3441 a         2605 c         2165 g         3777 t
ORIGIN      
        1 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
       61 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
      121 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
      181 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
      241 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
      301 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
      361 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
      421 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
      481 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
      541 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
      601 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
      661 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
      721 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
      781 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
      841 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
      901 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
      961 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
     1021 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
     1081 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
     1141 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
     1201 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
     1261 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
     1321 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
     1381 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
     1441 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
     1501 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
     1561 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
     1621 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
     1681 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
     1741 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
     1801 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
     1861 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
     1921 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
     1981 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
     2041 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
     2101 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
     2161 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
     2221 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
     2281 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
     2341 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
     2401 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
     2461 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
     2521 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
     2581 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
     2641 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
     2701 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
     2761 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
     2821 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
     2881 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
     2941 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
     3001 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
     3061 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
     3121 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacagttcca agtttcaatg
     3181 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
     3241 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
     3301 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
     3361 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
     3421 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
     3481 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
     3541 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
     3601 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
     3661 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
     3721 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
     3781 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
     3841 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
     3901 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
     3961 tgttgctgcc ttttagcata atactatctg ttgctgcaag attttgtttg atagcatcaa
     4021 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
     4081 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
     4141 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
     4201 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
     4261 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
     4321 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
     4381 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
     4441 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
     4501 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
     4561 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
     4621 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
     4681 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
     4741 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
     4801 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
     4861 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
     4921 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
     4981 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
     5041 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
     5101 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
     5161 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
     5221 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
     5281 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
     5341 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
     5401 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
     5461 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
     5521 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
     5581 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
     5641 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
     5701 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
     5761 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
     5821 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
     5881 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
     5941 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
     6001 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
     6061 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
     6121 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
     6181 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
     6241 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
     6301 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
     6361 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
     6421 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
     6481 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
     6541 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
     6601 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
     6661 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
     6721 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
     6781 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
     6841 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
     6901 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
     6961 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
     7021 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
     7081 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
     7141 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
     7201 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
     7261 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
     7321 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
     7381 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
     7441 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
     7501 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
     7561 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
     7621 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
     7681 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
     7741 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
     7801 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
     7861 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
     7921 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
     7981 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
     8041 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
     8101 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
     8161 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
     8221 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
     8281 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
     8341 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
     8401 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
     8461 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
     8521 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
     8581 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
     8641 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
     8701 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
     8761 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
     8821 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
     8881 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
     8941 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
     9001 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
     9061 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
     9121 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
     9181 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
     9241 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
     9301 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
     9361 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
     9421 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
     9481 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
     9541 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
     9601 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
     9661 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
     9721 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
     9781 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
     9841 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
     9901 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
     9961 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    10021 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    10081 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    10141 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    10201 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    10261 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    10321 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    10381 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    10441 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    10501 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    10561 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    10621 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    10681 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    10741 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    10801 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    10861 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    10921 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    10981 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    11041 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    11101 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    11161 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    11221 gtttttgacc aatgatcggc acaaaagctt ccggccatac ttcttgtatg actgccatca
    11281 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    11341 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    11401 ccgaccatgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    11461 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    11521 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    11581 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    11641 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    11701 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    11761 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    11821 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    11881 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    11941 acatctatat tccccacatc ttttttcatt tattaaaact gcctcctt
//
LOCUS       DACEND010000084        11982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEND010000084 DACEND010000000
VERSION     DACEND010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.086"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..2039
                     /locus_tag="IW746_001890"
     CDS             21..2039
                     /locus_tag="IW746_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1078788.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2466..4880
                     /locus_tag="IW746_001891"
     CDS             2466..4880
                     /locus_tag="IW746_001891"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1078789.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5228..6781
                     /locus_tag="IW746_001892"
     CDS             5228..6781
                     /locus_tag="IW746_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1078790.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7162..8946)
                     /locus_tag="IW746_001893"
     CDS             complement(7162..8946)
                     /locus_tag="IW746_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078791.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8961..10682)
                     /locus_tag="IW746_001894"
     CDS             complement(8961..10682)
                     /locus_tag="IW746_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078792.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10782..11543
                     /locus_tag="IW746_001895"
     CDS             10782..11543
                     /locus_tag="IW746_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1078793.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3926 a         2168 c         2428 g         3460 t
ORIGIN      
        1 ttgaagttgg aggatagaag atggaaaaca ttgcaagaaa agaacggaga cgtttagaac
       61 agaccaaacg ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact gcgatggtag
      121 gctgttctgt cgctgcccca ttagtacagc cggttcctgt tcaagcggat gaaacacctg
      181 ttcagttcgg cgcaagaatc aatacagcgg catttatcac agaaattgct acttatgctc
      241 aaccaatcgc tcaagccaac gatctatatg catcagtgat gatcgcacaa gcagttgtag
      301 aaagcggttg gggcagtagt gcgttatctc aagcacctta ttataatctt tttgggataa
      361 aaggcagcta tcaaggacaa accgtctaca tggatacgct tgaattttta aataatcaat
      421 gggtaaataa aaaggagcct ttccgacaat atccttcttt tgctgaatct ttcaatgaca
      481 atgcctatgt acttagaaat acttcatttg gcaacggtta ttactatgcc ggtacttgga
      541 agagcaacac gaaatcctat acagatgcga cagcttgttt gacagggcgt tatgcgactg
      601 atcctggtta cgcaggcaaa ttaaataata tcatcgcaac ttacggattg acccaatatg
      661 atacaccagc tagcggaaac gcaggcggtg gagcgactac aggtggcaat aatactggaa
      721 gtgcaggtag tactggcggt tcaagcaatt ctggtaatac aaactcaggt tcagcaactt
      781 cgaccactta tactgtccaa tctggtgatt ctgtttgggg aatctcaaat aaattcggta
      841 tcacaatgga tcaattgatc caatggaaca acatccaaaa caatttcatt tatccaggac
      901 aaaaactgac aatcaaaggt agccaggaaa atggctcttc gacgaataat tccggtaaca
      961 ataccaactc ttcaggaaat gctggtacat caaacggagg acaaacaact ggtgcgaaat
     1021 acactgttca atctggtgat tctgtttgga agatctcaaa tgatcacgga attacgatgg
     1081 ctcaattgat tgaatggaac aatatcaaga acaatttcgt ttatccagga caacagctaa
     1141 tcgtatcaaa tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata
     1201 caagtggctc tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata
     1261 cagttaaagc cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc
     1321 agctaatcca atggaacaat atccaaaata actttatcta cccaggacaa caactgactg
     1381 tttcaaacag tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa
     1441 ataacagcag ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag
     1501 caggtgaatc tgtttggagc gtggcaaata aaaacggcat cacgatggat cagttaatcg
     1561 aatggaataa tatcaaaaac aacttcattt atccaggaca gcaattgatc gttaaaggtg
     1621 gaacttcggc aaatacaaat acaggttcaa ctacttctgc agcgaaacca aatacaccga
     1681 atacgtctgc tacgacaagt acatcatcca ctggtaatac gatgtatacc gtgaaagcgg
     1741 gcgagtcagt ttggagcgta gcgaacaaac atcatatcac gatggatcag ttaatcgaat
     1801 ggaataatat caaaaataat ttcatctatc caggacagca agtgattgtg aagaaaggtt
     1861 catctcaatc aacaaaccaa caatcagtta ctggttcgaa aacctatact gtgaaatctg
     1921 gagaatctgt ttggagtgtt gctgacagtc atggaattac aatggctcaa ctaatcgaat
     1981 ggaacaatat taagaacaac tttatttatc caggtcaaac gttgattgta gctaaataaa
     2041 atagctgttc gaagaaataa cgattgaatt ccacaaaaat atttgttact ataaggctac
     2101 ttaaatatga caacacttga aaaggaagta gtaaaaagac tgaagtttca gagagcgcat
     2161 ggctggtgag aatgtgcacg gatgcttttg aactcgcctt tgagttgccc ttgctgaata
     2221 cccgagtaag caagcggcgg tgacggccgt tacaacgttc gaggcctttt cactaaggta
     2281 aaattaggtg gtaccgcgta gattacgtcc tataaaaaga gcaatctttt tataggattt
     2341 ttttatttct tttgtgaagt ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt
     2401 ctgccggttt tttcgttata tttataatag aaacaagtgt tttcaataga acaggaggac
     2461 agcaaatgag ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa
     2521 cgaatacttt caacacccat gatgatcctg aaaagccaaa attttacgca ctggatatgt
     2581 ttccctatcc atccggtcaa gggctacatg tagggcatcc tgaaggttat acggcaacgg
     2641 atatcctttc acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca atggggtggg
     2701 atgcattcgg tctgcctgct gaacaatatg cgctagatac aggaaacgat ccagctgagt
     2761 ttacccaaaa aaatatcgaa acattccgac gccaaatcaa ttctttagga tttagttatg
     2821 actggaatcg cgaagtgaat acaactgatc cggagtatta taaatggaca caatggatct
     2881 ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga agtagcggta aactgggtac
     2941 ctgaattagg aacagttatt tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg
     3001 gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact gcttatgctg
     3061 atcgcttatt ggatgatcta gaattagttg attggccaga aagcattaaa gaaatgcaaa
     3121 gaaattggat cggtcgttca gtaggagcaa atattgaatt taaagtggct ggtacggata
     3181 aatcctatac cgtatttacg acacgaccag atactctatt cggtgcaact tactcggttc
     3241 ttgcacctga actagatctt gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag
     3301 aagcttatat cgaagagaca gctaaaaaat cagatctaaa gagaacggat cttgcaaaag
     3361 aaaaaacagg agtatttact ggtgcttatg caatcaatcc agttaatggc aaggaaatcc
     3421 caatctggat cgccgattat gtattatctt catacggcac aggagccatc atggctgttc
     3481 cagcacatga tgaacgtgac tttgaattcg ctcaaacttt cggactagaa attcttccag
     3541 ttatcgaagg tggagatgtt gaaaaagctg cttacacaga agatggtacg cacatcaact
     3601 ctgaatttct agacggcatg aacaaacaag aagcaatcga taaaatgaat gaatggctag
     3661 aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt acgtgactgg ctattctccc
     3721 gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg ggaggacgga acagtcacag
     3781 ctgtcccaga agaagagcta ccattacggt tgcctaagac aaaaaacatc aagccaagcg
     3841 gtactggaga atctccatta gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa
     3901 caggtaaaaa aggccgtcgc gaaacgaata caatgccaca atgggcagga agctcatggt
     3961 attacctgcg ttatatcgac cctcataaca aaaaagaact agcagattat gaaaagttga
     4021 agcgttggct accagttgac atctacattg gtggagcaga acatgcggta cttcatttac
     4081 tatatgcacg attctggcat aaattcttat acgatatcgg tgttgttcct acaaaagaac
     4141 cattccaaaa actatacaat caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa
     4201 aatctcgtgg taatgtagtc aatcctgatg atgtggtcga aacttacggt gcagatacac
     4261 tgcgcatgta cgaaatgttc atggggccat tagatgcttc gattgcttgg agcgaaaatg
     4321 gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca
     4381 ataaaatgcg tgaccgtatc actacattga atgatggcaa gcttgataaa gtgtatcatc
     4441 aaacagtgaa aaaagtgaca gaagactatg aaaatcttca tttcaataca gcaatctccc
     4501 aattgatggt ctttatcaat gaagcctata aagtggacgc tctgccttat gaatatatcg
     4561 aaggatttgt tcaactactc gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa
     4621 tccttggaaa tgatggtggt atttcttacg caccatggcc aacatacgat gaagcagcac
     4681 ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca
     4741 atgttccccg agacttagga aaagatgagc tagaaaaagc agcattagcc aatgaaatag
     4801 tgcaagaata tattgaaggc aaaacagtca gaaaagttat tgcggtacca aacaagttag
     4861 tcaatatcgt tgctaattaa ttggctcttt gtcaataagg actgatgagt tgtgcaaaat
     4921 caaatctggg ccagaatgaa cttcattctg tctcagattt tttgttattt caaattgatt
     4981 aattgattga tcaaaaaggt tttgattttc atgttctcaa acacgttcaa tgtctttttc
     5041 ttctgaatgg tcttgtaaga gatctctgta atcatcaaat ttttgtctat taatcttagt
     5101 attttttgat gtataaacta cctgaaattg agagtacata ccaaattcat aaatttttta
     5161 ttagtgggac tagtgattgt atggattttg acgcataatg aatgaagtat gagaaaagga
     5221 gagacaaatg gatcaagaac tatttattat cgaagtatta tcagctaaac aaaaaattat
     5281 cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag cttttaagct tagtattttt
     5341 ctctggtttg ctttttttcg gagctgtatc gttcctatat cgccatatga gtgcagtagt
     5401 ccgttcagct gaagggatgc aaaaattaaa taatttgtat cacttttcgt ggggagttgg
     5461 cggaataaca attgttgtgc ttattgtttt tcttctactt atttggctcc gttaccatca
     5521 attgtcgcgt catttcgatc aatggcttag tcaaagagca caacagtttt tacataataa
     5581 accaacttct caagatgatg actatcttta ttttgagaag acttcgacga gtcaagcttt
     5641 tcggatacaa aaaagtgatt gccagttatt gctgagatca cttgatgatt tacctatcta
     5701 cttaggcgaa agcaaatcaa aattcggaat gaacacattc catatttttt tactagaaaa
     5761 cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga tacctgacaa ctaagatgag
     5821 tacattagtc actggttttt tcctcattct tttagctgga acaatctatt atgggagata
     5881 cactcagcca aaaatgtctt ctcatataaa tgctgcttct cacatgacaa tgaactcaac
     5941 taacgatacg catatacaaa agacaaatga agggttgtta gctgatccta agcaaaaggt
     6001 aaatttgaca acacaaaaga taaatgatct caaacttgat acagagacgc actctctcta
     6061 tatgacaaca gatagtggtg ctaattgggt atttgtgccg attgattcgc aatggttgcg
     6121 ttttggcgaa tacaccctga caacggggct gattcctgaa ggttattgga tggacaacac
     6181 atatgatatt tccgccgatt tctcatggtt tatctattct tctggggatg atgtacgtct
     6241 gctgacttca agagataatg gtaaaacatg gcaaacgtct attatcaccc aatcagttca
     6301 gccgatacgt tatcgaaaag cggtatttta tggcaacgat ggtctattta tctgtacatc
     6361 agccagtggg atgtctgcag aaactttgta catttacact actatggatg gcggaatcac
     6421 ttggataaaa agtggaagta caatgatcaa tcagccagta caaaatgtca gcttcgttac
     6481 aacttcttta ggttttgttt cgacacgtga aagtttgttt tatacaaaca acggaggacg
     6541 ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat cagacagggg ggatagatct
     6601 ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat acgttagaga caaaatttta
     6661 tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt gcctgcctct tccgttcaac
     6721 tgataacggg gaaacatggc aattcgttca gcaactatcg caagtagaac aaggagatta
     6781 aagaagagag attgtgagcc tgacgttcag caccgagtca caagaaacaa attcaaaaaa
     6841 cagcttctca tatttgttga atagtgtaac tcgtgattcg gagttgacaa gagcaacaag
     6901 aagcgatttt gaagtatagc ttctcgtatt ttacaagatc gtgtcttatt gccgataggt
     6961 tgtctcttga acaccgttta ttcgtaaaaa gggattgtga caagtctttt gtcactactt
     7021 accgaacgaa actgaaggag ctatccaaaa agtatcatta gtattattct ttgtgtttct
     7081 agtggcacca ctagtacatt taaaaaagct atcgatcatg gactaatcat acatgatcga
     7141 tagcttttta gataatgttt gttacgctat ctgaaattga ctattataaa gactggcata
     7201 aagcgttggt gattggagta gttcagaatg tgttcctttt tcgacgatat cgccattctt
     7261 cattacaagg atcatgtctg cattttcaat cgttgacaaa cggtgtgcaa tcacaaaact
     7321 tgtcctatct tttgtcacag cttccattgc ttgctgaatc aatgcttctg ttcgtgtatc
     7381 tacgctagaa gttgcttcat ctaaaataac gacaggagga ttcgctagaa tgactcgagc
     7441 aattgtcaat aattgttgct gtccttgaga taacgtgccg ttttcgctag aaatgatcgt
     7501 atcatatccg tctggcagtg ttcgaataaa gtgatcacat tgtgcgattt cagccgcttc
     7561 gataatttgt tctcttgtgg catccatttt cccgtaggcg atattatcag ctaccgttcc
     7621 ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa cgacgtagtt ctccacgtga
     7681 aagcttggta atatcaagac cgtcgaattc gatctttcct ttattcagtt cataaaaacg
     7741 catcagcaga ttgactaaag tcgtttttcc tgcaccagtt ggaccaacga tcgctaccgt
     7801 ttgttttggc tttactgaaa aatcgacttg attcatcaat attttatctg atgtgtaacc
     7861 aaactgtaca tttttgaaag ctatttttcc tttcggttgt tctaaaagtc ttgcatctgg
     7921 aaggtctgga atttcatctg tttcatctaa tatctcaaat atcctatcga ttgctgccat
     7981 tgcggattgg atcgagttga tcacataaga agcggtagaa attggttcag aaatctgatt
     8041 gatgtactga agataagctt gtaagaagcc gatcgtcaca ccacctgata gaacaagcat
     8101 agcaccgaat accgcactta tgatgaaggc gagttgatta atgaaacgaa tggccggata
     8161 gatagcacag ttcataaatt gagcacgttt aaaagcgttg tattgctttt tatttacttc
     8221 gtgtatagca gcttctgcat gagcttgctg attgaatgtt ttgaccacta gattaccagc
     8281 taagatttct tccattcgat tatttaaaac acccagttca tactgattct gttcagaaat
     8341 ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa ctgccaacga tcagcaataa
     8401 gacgagcaaa gtaagcttgc tttcgatata aaacagcatc agaccagcag cagctattgt
     8461 tacgacagaa gtaaaaaatt gattgatacc tgtcaataac acttgagaaa gttgattgag
     8521 accggtagtt gtccgactaa tgatgtcacc tacttgatga ttgtcaaaaa acgacatcgg
     8581 taatgttttg aatttttttg tgatttcttt tcgcaccctc aatgtgatct tttcgcttaa
     8641 cgctgccatt gtccgttcct gaataaatga agtgatgctg cttaagactg aacagccgat
     8701 cagcaataag acagggaaca acaatgcttg tttgatatca ctgaaagaaa gctgtccaat
     8761 acctgttttt cttatttctg ataagagatc atcgatcccg attcccatga tcaagggcat
     8821 ggctacgact aataggttcc cgaataaact gcatagaaac aatccgtaaa aatatttatg
     8881 ttcaggtttg atcattttcc aaaaacgtat caaagcacgc gtgttcaacg tttttttctt
     8941 ttccatcgta tcccttcttt ctatattcct tgtgattttg caaactcctg ataagtttgg
     9001 caattagaca gtagttcttc atgagtacct tgtccaacga tttttccttc atctaaaaca
     9061 atgatgttat ctgcttgttg gattgtactg aggcgttgag caacaatcac gagagttttc
     9121 tccgacatgt gtttatgaag ggctgctctt aatgctgcat ctgttttata gtctaaagcg
     9181 gagaaactat catcgaatac atagatctct gctggtttga tcaacgctcg agcaatacat
     9241 atccgctgtt tctggccccc tgaataatta gaaccgcctt gagcaacaaa gctgtccaat
     9301 ccgtcaggaa gggtgtccac tagttcttct aattgagcga tcttgatcgc tttagacaac
     9361 tcttctgttg aggcatattc atttcccatc tgcaagttgc ttaaaattgt cccgctgaat
     9421 aagaaagcct tctgagggac ataactgatg tgggagcgga tctgttcttg agtcatttga
     9481 gtaagaggaa taccaccaag ggttactttt cctatcgtag ggtcactaag tctcaagaga
     9541 agtttcgcca aactactttt tccagatccg gtcccgccaa cgatcgctgt tgtttttcct
     9601 ttaggtattg tgaaatcaat atcttctaga actggatcag agatttcagt atatttaaat
     9661 gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg ttggttcagc tactgttttg
     9721 atcgtctctt cctcaatttc gatctctgct tgcagtattt cttctatccg tcgtaatgag
     9781 gcaaacgatt tagggataat gaccaatacc atagctgcga tcatcaagta agcaagtgtc
     9841 agcatagagt attcgactac agcagtcacg acaccgattt gtaatgctcc ttctgctaca
     9901 agatttccgc caaaccacaa aatagaagca taaacgattc ccatgagcaa aaaagcaatt
     9961 ggagtgaccc aagcaaatat tcggttcact ttgatcattt gattagcata gtcagcaaag
    10021 ctcttattcg ttcgcttttc ttcatatgat tgattatcaa aggcacggat catattaatc
    10081 ccagtaaaga attctctcaa tgtgatggtg atccgatcca tttttggctg gatactcaaa
    10141 gataaaggtg cccctcgttt catcagtcga taaacaatcc atacataaaa aacaacagag
    10201 atcaatgtaa cccatgttag taatagagaa taacgaaaca tcatgagaat ggtgaaaacg
    10261 caaataaccg gtgctggtaa gatcatttgg caaaataaaa caatcatttg ctggacatta
    10321 tcgacatcat tcgtcattct tgtcaaaaga gatccagaac taaaacgact gatatccttc
    10381 attgacagct gttggaattt acggaaatat ttctcccgcg tttggaatcc aaacctagcg
    10441 gaagccaatg ctgaaaagta actagaggca actgcagcga ttgttcctag aaacgctaca
    10501 agcagcattt caactccaat tttttttata actgaggctt cgccacttgc tatacctgtg
    10561 tcgatcagct ttgctactaa ttttggtaca cctaatgtac cggcgatttc taaacagatc
    10621 atcacaaagg taaggagagc gagtcctttg tttttctgta agaatgtttt taagatagac
    10681 atattattcc tccttttttc ctgatacttt tcgattctat accttcaagt aacttgaagg
    10741 tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa aatgaaaaaa atgtatacga
    10801 ttggccaagt atcggaacta tttgatttgc caaaatcaac gatccgctat tgggatgaac
    10861 aaggattaat ccgttcaagt agacaagaag agaatgatta tcgattgttt gatatcgatg
    10921 atatttttat gctttatgat atcgtttttt atagaaaatt agatataccg atcaaacaga
    10981 tgaagaattt atatggaaaa acattgacgg agttatatga gacacttgat gaaacagaaa
    11041 gaagaatcca taaagaactg gtggtgatga aacaaaaaca aaaagagata agggagcaaa
    11101 aaaaacagtt gaaattgatg atcgatacaa acgaggaaga atttcctgta gaagagatcc
    11161 cttttgactg tatgatctca acggagtttg aagatattgt ggaaatcaaa aaatttctac
    11221 ctaactattc aagctttggg atgatgagta tgccagcttc cagttcacag acgatgtatg
    11281 gattttttat cgaccctagt gaagtacatt tatttactca agacgtgatt tgggagaaaa
    11341 aagatacggc tgtttatcgt cgattcttat tgaaatccga gatgaaccat gcggaacgta
    11401 ataatatttt agaaatcaga gaaagaatgt atgaaaaagg atggaaaaca ggagaagtga
    11461 tcggccagta tctattgacg aatacggatg aaaacaatat cagaactgaa tattatcatg
    11521 catggataga aatgaaaaaa taggaaatat gtgtttaaga taactcgaaa atgatggttg
    11581 ttttagttat aaagaaaaat tttctggttc gattacgggt ttaatgtggg ggattattag
    11641 tcgtggatag atagaatttt agttttttag aggaaatatg tctttaaaaa tggaatggtt
    11701 gcaattctta gcgatatata cgatgtaatt gtcctcttaa aaaagtatgg aaaaataatt
    11761 gtattgggtg ttatgtataa ttcatggtga actgagacta atgagaagaa aaattcatat
    11821 tgttatgagt gagattgtat cagatgagaa tgataaatta aataggtgca taaaagcatt
    11881 acacttaaaa tacattgttg cttacattat tacaatatgt taataaggca aaaataaata
    11941 gatggattga ataaacatat tttttaatca aatagtccct ac
//
LOCUS       DACEND010000085        11885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEND010000085 DACEND010000000
VERSION     DACEND010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.087"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..2714)
                     /locus_tag="IW746_001896"
     CDS             complement(3..2714)
                     /locus_tag="IW746_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1078794.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLNKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(2724..3425)
                     /locus_tag="IW746_001897"
     CDS             complement(2724..3425)
                     /locus_tag="IW746_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078795.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3611..4210
                     /locus_tag="IW746_001898"
     CDS             3611..4210
                     /locus_tag="IW746_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1078796.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4291..5304
                     /locus_tag="IW746_001899"
     CDS             4291..5304
                     /locus_tag="IW746_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1078797.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5412..6329
                     /gene="rbsK"
                     /locus_tag="IW746_001900"
     CDS             5412..6329
                     /gene="rbsK"
                     /locus_tag="IW746_001900"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1078798.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6357..6752
                     /gene="rbsD"
                     /locus_tag="IW746_001901"
     CDS             6357..6752
                     /gene="rbsD"
                     /locus_tag="IW746_001901"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1078799.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            6752..8125
                     /locus_tag="IW746_001902"
     CDS             6752..8125
                     /locus_tag="IW746_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1078800.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8482..10626
                     /locus_tag="IW746_001903"
     CDS             8482..10626
                     /locus_tag="IW746_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1078801.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10678..11718)
                     /locus_tag="IW746_001904"
     CDS             complement(10678..11718)
                     /locus_tag="IW746_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1078802.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         3629 a         2403 c         2251 g         3602 t
ORIGIN      
        1 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
       61 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      121 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      181 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      241 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      301 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      361 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      421 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      481 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      541 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      601 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
      661 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
      721 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
      781 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
      841 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
      901 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
      961 gtaacgattg cttgatagtc aatgatcgtt ccaaattgtt tggctgaaac agaacttaga
     1021 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1081 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1141 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1201 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1261 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1321 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1381 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1441 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1501 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1561 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1621 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     1681 tttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     1741 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     1801 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     1861 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     1921 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     1981 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2041 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2101 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2161 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2221 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2281 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2341 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2401 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2461 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2521 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2581 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     2641 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     2701 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     2761 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     2821 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     2881 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     2941 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3001 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3061 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3121 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3181 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3241 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3301 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3361 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3421 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3481 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3541 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3601 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     3661 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     3721 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     3781 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     3841 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     3901 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     3961 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4021 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4081 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4141 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4201 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4261 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4321 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4381 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4441 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4501 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4561 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4621 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     4681 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     4741 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     4801 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     4861 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     4921 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     4981 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5041 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5101 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5161 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5221 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5281 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5341 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5401 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5461 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5521 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5581 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     5641 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     5701 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     5761 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     5821 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     5881 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     5941 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6001 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6061 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6121 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6181 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6241 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6301 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6361 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6421 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6481 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6541 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6601 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     6661 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     6721 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     6781 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     6841 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     6901 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     6961 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7021 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7081 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7141 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7201 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7261 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7321 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7381 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7441 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7501 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7561 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7621 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     7681 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     7741 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     7801 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     7861 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     7921 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     7981 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8041 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8101 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8161 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8221 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8281 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8341 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8401 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8461 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8521 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8581 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     8641 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     8701 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     8761 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     8821 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     8881 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     8941 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9001 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9061 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9121 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9181 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9241 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9301 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9361 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9421 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9481 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9541 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9601 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     9661 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     9721 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     9781 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     9841 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     9901 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     9961 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10021 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10081 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10141 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10201 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10261 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10321 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10381 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10441 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10501 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10561 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
    10621 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
    10681 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
    10741 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
    10801 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
    10861 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
    10921 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
    10981 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
    11041 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
    11101 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
    11161 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
    11221 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
    11281 tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa
    11341 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
    11401 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
    11461 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
    11521 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
    11581 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
    11641 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
    11701 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagc
    11761 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
    11821 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt
    11881 ccgaa
//
LOCUS       DACEND010000086        11882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEND010000086 DACEND010000000
VERSION     DACEND010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.088"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..844
                     /locus_tag="IW746_001905"
     CDS             236..844
                     /locus_tag="IW746_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1078803.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1012..2649
                     /locus_tag="IW746_001906"
     CDS             1012..2649
                     /locus_tag="IW746_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1078804.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2721..3833)
                     /locus_tag="IW746_001907"
     CDS             complement(2721..3833)
                     /locus_tag="IW746_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1078805.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3908..5281)
                     /locus_tag="IW746_001908"
     CDS             complement(3908..5281)
                     /locus_tag="IW746_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1078806.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5502..6119
                     /locus_tag="IW746_001909"
     CDS             5502..6119
                     /locus_tag="IW746_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078807.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6217..6672
                     /gene="argR"
                     /locus_tag="IW746_001910"
     CDS             6217..6672
                     /gene="argR"
                     /locus_tag="IW746_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1078808.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6690..8192
                     /locus_tag="IW746_001911"
     CDS             6690..8192
                     /locus_tag="IW746_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1078809.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8287..8493
                     /locus_tag="IW746_001912"
     CDS             8287..8493
                     /locus_tag="IW746_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078810.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            8579..8980
                     /locus_tag="IW746_001913"
     CDS             8579..8980
                     /locus_tag="IW746_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1078811.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            9135..10466
                     /locus_tag="IW746_001914"
     CDS             9135..10466
                     /locus_tag="IW746_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1078812.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            10488..11114
                     /locus_tag="IW746_001915"
     CDS             10488..11114
                     /locus_tag="IW746_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1078813.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            11297..11878
                     /locus_tag="IW746_001916"
     CDS             11297..11878
                     /locus_tag="IW746_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078814.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
BASE COUNT         4106 a         2140 c         2489 g         3147 t
ORIGIN      
        1 agaatctgcg tttttcattc aaaaataaaa aaaacataga aaaacgatta ctttgcttat
       61 acaacaaggc ttttatggtt ttgtatcaaa aaaatatttg tttaaatgtt acaaatatta
      121 aataattcgt tattttgtca ttaaatgtca tgtttctttt acctatgaaa tatttctaaa
      181 tgataaagtt aatagcgtcg aaagaataaa aaacggagaa aaaaaggagt ttaatatgaa
      241 ttcatttaag aaaattgttt taggtacaac ttttgctgct ggtgctactg caatgtttgt
      301 aggaactaca aatgctcacg ctgatgaagt ttatacagta aaatcaggtg attcattatc
      361 taagatctca caaaaatttg caggagacaa ctcaatgatc gatgcaattg ctgaaaaaaa
      421 ttcgatcgca aacatcaacc gcatttatgt tggtgaacaa ttaacaatcc caacttcaaa
      481 tgattcttca gcaacaacag aaaacaaaac aacagaaaat acagcatcta caactgaaac
      541 agcaacgcaa gaacatacat acgttgcacc tgttgaaaca gttgaagtag ctcctgctgc
      601 cccagctgct gcaacagcgc caacttcaag ctctgcaaaa gaatggatcg cacaaaaaga
      661 atcaggcggt tcatacacag ctactaacgg acgttacatc ggccgttatc aattagatgc
      721 ttcatactta aacggcgact attcagctgc caatcaagaa cgtgtagcag aacaatatgt
      781 aacatctcgt tatggttcat gggatgctgc taaaacattc tggttagcaa acggctggta
      841 ctaagccgat aaaaggaaaa tgatgaaacc gagaaacttg tttattcaag ttctcggttt
      901 ttttgtgtga aaaataactc gtttgcttcc cacagcgcag cagtacaaat aaaaactcga
      961 ataatgtata ataaagaaaa cgtttttttg tttagggaat gaggaggagc catgagtaca
     1021 aaagagagtc tcgaattaaa cgaaatcaca agtggaaatc cactgttatc cagtattcga
     1081 tcgattgtag aaacgacatt ttacggtaat aatgttcatg aagtttttga taggaagaca
     1141 gcttatcaac tagcaaaagg gagtccagga acgatcatca cagatttaac aatctcacat
     1201 gctgaagaac tagatttacc agctgatgtt aggacactag tattcaatga tggaagcatt
     1261 gtgggaagaa cagcaagtgc tcggcggatc tttgaggact tagacaagga acaaagcaaa
     1321 tacgagaaaa ttttaagaga agctgtgtat caaagtcgca agcgccagtt ttaccataca
     1381 aaagtaatcg ttggcctatc tgaagaattt tctgtgcaaa gtcatttgct gattccagaa
     1441 ggatttgaga acaatttgta ttcttatttg ctgaattttc agccagcgaa ttcgaagatc
     1501 ttagagactt atcaaaagtc tgtttccttt gatgagggag atctttatat cttttgtgat
     1561 cccgaatgga cacatgaaac atttccaaat ggactcattt tgtttgatgc tctccataat
     1621 acagcggttg tgctaggatt acgttacttt ggagaattaa aaaaagcgac tttgacgctt
     1681 gcttgggcat tgggaaatcg caatggcttt attgcctgcc acggtgggat gaaacaatat
     1741 gctagtaatg accagaccta tacgatggct gtatttgggt tgtccggttc tggaaaatca
     1801 acgattacct tagcaaaaca tacggaaggt cactctgttt cgatcttgca tgacgatgct
     1861 ttcgttattt ctcgaaaaaa tggttttgct actgccttgg aaccttctta ctttgataaa
     1921 acacaagact atccgtctga tgatccggcc atttcttctt tccttacttg tcagaatgtg
     1981 ggcgtgacat gtacaagtaa aggagaaaaa gtcctagtaa caggagatat acgaaacgga
     2041 aatgggcgga cagtcaaatc acgttatgcg acacccgacc gagtcgatca tcttagcgaa
     2101 aagatcgatg ctgttttttg gatcatgaaa gatgattcgc ttccgccaat tatcaagatc
     2161 aatgacccag ttttagcagc gtcttttggt gcaacattag caacaaaacg atcagacgcg
     2221 gaaaatatca ctacaaaaag acagctagat gagctagtca ttgaaccatt tgctaatcct
     2281 ttccgtgtct atcctcttag ggaagactat caagcttttc gagagttgtt tttgaaacag
     2341 caaacagcct gctatgtcct gaatactgga tatttcgatg ataaaaaaat caaacctgaa
     2401 acgacattgt caatcgttga aagaatcgtg caacaaacgg cagaattcaa gtcgtttggt
     2461 ccatttgaag acatccacta tttacctata ccagattttc ctgtttcttt tgaggatacg
     2521 tcctataaaa atctggtgaa acaacgactt gatattcgtt tacgattcat tgaagctcag
     2581 ataggaaaag atctcttgcc agatgaagca aaagaaagtt tggagaattt gattcagaaa
     2641 ttgaactaaa ataacagctt tttcaagaag taatccatgc gcaatatcgc tcgggatcct
     2701 tacttgaaaa agctgttttt ctatgatgga tttttcgtaa tataggtgga gatgtccttt
     2761 tgataatctt ccgtaggcca atgaccagta tttaagaagt tggctaatag atagccgata
     2821 taaggacctg tggtcaagcc agaagagcct agaccgctag cagccagtaa gcttggatca
     2881 tctggcagta cgccgaaaaa tggggaaaaa tctgacgtat aagcacgggt acctacacgg
     2941 aactcgtatg gccagttttt tatctgatct ggatcagtca agaactgttc tgtactctta
     3001 gtcaatccct tataagcttc gctagtagga gccaaatccc agccttgatc gttttcatgg
     3061 gtagcaccta aaaggatctt tccatccatg aaaggaatga aatcagcttc tccgtcaagc
     3121 atagcaacag gccagtcttg actcatctta taagatgtct gaaatgctaa gagctgccct
     3181 ttttgcggac gtatatctgc agcatagcct aattcttgta ataaaggctt aagtgccggg
     3241 ccaggtgtca aaattacacg atcagctgtg tattctccaa acggtccctt gatgagccaa
     3301 gaatcgctct tcttatgcaa agtgactttt gcctggatct gctccatttg tttttgtgct
     3361 aaacgctgac caagatgttc taaatatgct tttccatcca gacgaccacc accactgata
     3421 cgcagtgaaa ctgctggttt caataacgga agtgcttgag atgtctgttc aggtgaaagc
     3481 cattcgatgt ttccaatttc tggcgcttct tcttttcgtt tctcggcaag aacagccaat
     3541 tcagctaaat ctttttcagg tcgaagcaaa agtgtccctg attgtgcgta aatcgtttga
     3601 tcaagttgga aatcttttat aagtttaggg aaaaaagcag cgccgtcttt agccagacga
     3661 taccattgtt tgtttctgcg tttcgacaac caaggggaga taatcccagc gctagcttta
     3721 gttgcctgtc ctgtcccttc atcaaataaa gcaacattga attttgatgt atctaagtaa
     3781 ctggctaggg tcataccaat gatcccgcca ccgataatgg caattttttc cataaaaaag
     3841 cctcctttta caaacaacca ttttcacaca aaaaaacggc agatgcaaca cctgccgtat
     3901 atggaatcta cgctttttta gcaaagaaca gataataggc gaatcgagca aagcttacga
     3961 gaagcaacag caacaagacg cctaatgctg cttttgatcc taatgtagta gacacttcat
     4021 agttatggga agtatgatgc tggacgtaat tgataatgac cgctactaat gaagtagcca
     4081 cagcgccaga aaactgctgc aaggtattga acattgcgtt cccgtctcct tgtagttctt
     4141 cattcagtgc attcattccg gtggtcatca tattgctgta agataatccg ataccgatca
     4201 tataaaacac atgacaagca atcaatacac ccactgaagc gtgtcctatg agtaggaata
     4261 atgctagcca accaatcaaa gaaagaatca gcccagcaaa aattggcttc ttcggaccat
     4321 attgatccag cattttcccg gaaaaaggag aaagcaaagc accaaccgct gcgccgggaa
     4381 gcatcgctaa tccagcgaca aaaggactac ttccttgaac gatctgaagg aaattcggca
     4441 agacaaaaga tacaccaagt aataaaaact gataaactaa gaagccaatc aaaaacaagc
     4501 ggaatgtttt atttgaaaga atagacagtc ggatcagtgg atgctccact tttttacttc
     4561 gatagtaaaa aaggaaccag ccaataccgc cgatgattaa agcaatccag ctggaaactg
     4621 ttcccaacgt actcaaaaat gttagacttc cactaaacaa aagacaaatt cctaaaacgc
     4681 ttatccaatc caaataacca cttttttgta ctgccatctc gggaatagca aaccagccga
     4741 taatcaaaga aagaatcaaa acgggcacaa gaaacaggaa gatatgattc catgtaaaat
     4801 gtgaagtcat aatgcctcca taagtggggc caattgctgg agcaatggcc gttgtcaaag
     4861 taccgacacc catcatcgcg cctcgtcgat tgaggggggc aaatgtcaag ataatatgga
     4921 acatcaacgg aagagcgata cctgtcgctg caccttggag caatcgacca aacaatagga
     4981 ccatgaaggt aggagagaag aaatcgatca ttatacctgc caagaaaagc agatttgaag
     5041 ctaaaaagag ttttttgaga ctaaaattgc gattcaaata agtagacagc ggaacaatga
     5101 tcgaaatcat caaaagataa atcgttgtta cccattggac ttgtgcagtc gttaaatcaa
     5161 actgtctgat aagagttggg aaagttacat tcatggccgt ctcgatcaat acgcccgcaa
     5221 aagacatcat ccctgttgca attactgcag gtactaaaag cagctgtttt tgttttgtca
     5281 tttacatcac ctctaaaaaa tttcgtaaaa aaagagagtg atttctatta taaaaataga
     5341 aaccgcctct ctctcgacac gccgaaacgt gttatctgta cgatttattt acttcactta
     5401 ttctagcaca aactgaaaaa tagtgtcaac acgattagcc aagtcactca aaaagaggta
     5461 tgataataaa taactaaatg gaatttcgcg gaggaagaaa aatggaaaaa attgaatggc
     5521 gtaaagacaa gagctatagt atgaaaaaaa gcccgcaaat actagaggtt cctaaaaaat
     5581 cattctttag catcagaggg atcggcgacc cgaatcagga agactttaaa cgaagagtag
     5641 cctgtctata tgcagtcagc tatgcaatac gcatgtcgcc taaaaaaaat tggctgattc
     5701 ctgattatca gatgtatact gtctatccac tggaaggaca atggggactg caagaaaagt
     5761 atttgcatga accagtgatg aaaaaagaac atttttctta tcaattgatg atccgacagc
     5821 ctgactttgt tacagaagcg atagcacaag aagccattca gcgttctagc aaacttccgg
     5881 aagacttgaa agaacaagta gtattcaaaa agatggaaga aggtctttgt gcccaaatct
     5941 tacacgtcgg gccctatgcc gaagaaccag caacgtttga gaaattagaa gcattcatcg
     6001 caaaagaagg ctatgaaaga gcttctaaag aacacaaaga gatttacatc agcgatccaa
     6061 gaaaatcagc acctgaaaaa atgaagacga ttttgcgtgt taaaatagaa aaaggttagc
     6121 aacctgaatg aaaacaaaaa ataaaagtaa aaaataccaa aatatgaaga aatattactt
     6181 atcttttgct ataatcaaca ttgagaggtg tctaatatga aaaagaaaca gcgacaagca
     6241 ctgatccgcc agatcattac agaacaacct attgggacac aggaagagct gcttgcgcgg
     6301 ctgcatgaag caggcgccga tgtgactcaa gcaacgattt ctcgagacat tcgagaaatg
     6361 aagttagtaa aaagtcaaaa tgaaaacaaa atagtacgct atacattatt taaccagcca
     6421 tctgtttctt taaatgaaga acgtttgcgt acagcaatta gacgcgaggt tttgcgtatc
     6481 cagtctgttc agttcatggt tatcgtattg actgaacgaa atggtgccga tgtggtaacg
     6541 aattggctag atgaagtggc ttaccctgaa gttgtcggaa cgatggccgg tgtagataca
     6601 ttcattatta tttgccgctc agaagaagaa gcacagcgat ttgcagaaaa gctggaaaaa
     6661 atgagggaat aaatgaggag gacttttata tgaaagatat gaaagcgctg caaaatggat
     6721 cggatattcg aggaatcgca ttaccagttg aaggatatga agtaaacttg acgacagaca
     6781 tggtaaaaaa gatcggctgg ggactagcaa actggataaa gaaaaagaag aaatcaaccg
     6841 atccaaataa aaaatggacg attgggattg gtcatgacag taggataagc ggaccagcac
     6901 tgaaagaggc gttgatccaa agtctgaaag aacaagggat acatgtcatc gatttcgaat
     6961 tagctactac gcctgctatg tttatgtcca ctcaatttga acagttctcg tgcgatgctg
     7021 cggttatgct gactgctagc catcttccct attattataa tggaataaaa ttctttacct
     7081 cagaaggagg agcagaaaaa gaagatattg cttttatcct atcacagcag caaaaaataa
     7141 aaagcgaaca aactggaagc gtagaaaaag cagacctttt gacaccttat gcgcaagatt
     7201 tagtcacaaa aattaaaaag gggattggaa cactagaaag caaaccacta aatggttgga
     7261 aaatcattgt tgatgccgga aatggtgctg gtggattctt tgctgaaaaa gtattgaata
     7321 gcttaggtgc ggattcaaca ggttcccaat ttttggaacc agatggccga tttcctaatc
     7381 atatcccaaa tcctgataat aaagaagcga tggaaagtat caagcaggct gttttagcaa
     7441 atgaagcaga tcttgggatc attttcgata cagatgtgga tcgttctgca gttgtcagtg
     7501 cttctggaac agtgatcaat cgaaataatt tgatcgcagt attaagtaca atcgtattaa
     7561 aagagcatcc aggaacaaga atcgtgacga attcccctac ttcttctcat ctacaaagat
     7621 tcattgaaga aaaaggaggc atccaagttc gttatatttc cggctatcga aatgtaatca
     7681 ataaagcatt agaacttaac cgtcttggga aagatacaca gctagccatc gaaacaagcg
     7741 gacacgctgc attcaaggaa aactattttc tggatgatgg tgcatatgtc atcgcaaaga
     7801 tcctgatgct tttgccagaa ttaaaaagaa aaggtatcac attagatgaa caaatcaaag
     7861 agttgaaaca gcccgtagaa gtacaagaaa tccgattaaa attgttcagc gatgaaccac
     7921 gcgaacaagg aaacaagata ttagaagaaa tgaagcgact gttggaagaa gaaaaaggga
     7981 tgaacttcga cccggaaaat gaagaaggta ttcgatatga tctagaagag ccatacggaa
     8041 acggctggtt tttgatccgt ttaagcttac atgagccgtt actagtatta cagatagaaa
     8101 atgatgaatt cggaaagaac aagttggcgt tagtaaaatt agcaaaatta ttcggacatt
     8161 tttctgatct tgaatcatta acagcagaat aaaccagtct ttttgaaagg ctagttgctt
     8221 ggacggcaaa aaaagtggta tggttaaatc gagtgctggt agggagacca gcaaaaggag
     8281 gaaaaaatgg taacgaatat ggttattttt ttaacagtat tggtttatgg ggtcaacttt
     8341 ctggtatata tgtatcttaa aatcaatcat ataaaagatt atgtagaaaa gattgctatt
     8401 tattttggaa caaatatgac gctgttgttc acgagtagta tttttttgtt ttttggaaaa
     8461 atagtagaag acggcattct ggtattcgaa taagcagaat ttttgtagaa agcgccttac
     8521 ttgtcataca ctataattat agccaagtag caaatggtca atcgaaagga gcagtacaat
     8581 gaaaaaagca tacgaaacag caatgatcaa ccatggcggg cgcaatggag aagtcgaggc
     8641 accaaatggc tcgatgcata tgaaaatcac tcctccagga atccatgcag aaggaacaaa
     8701 tccagaacaa ttatttgcag caggttatgc ttcttgtttc aatggggcgg tacaacatat
     8761 gatcaaagaa gcaggaatga cagcagagtc cacagtaaaa gcacgtgtct ctctttataa
     8821 tttagatgat ggcggctatc agatcggtgt agtattagaa gtgcatatcg atggcatcag
     8881 cgaagaagaa gcccaaaaaa ttgcagaaga tgcgcatgaa ttctgccctt actccaaagc
     8941 aactagagga aatatcgatg tagaagtaac gattgtttag aagaataaag gaaatcgaac
     9001 cagactaaaa acgtgaaagg accgcttttt gtctggtttt ttttgacaaa taaagcagat
     9061 gatttggaga atagacaatt atcggttatc atatttttgt accaatcggt aggtaaaaaa
     9121 gaagcgaggg aaaaatgatg gaaaaatatc aaaatattgt gatcggtttt ggaaagggcg
     9181 gtaagacttt agcgaaggcg ttagcttcaa aaggtgaatc ggttttagtc gttgaaaagt
     9241 caacacgtat gtatggcgga acatgtatca acatcggctg tattccttct aaatcattga
     9301 ttttcaatgg agaaagagga atcgacttta cagaggctgt tgctcggaaa gaaaaattga
     9361 cgggcatgct tcgcgccaaa aattatcata tgatcagcga tgaggcaact gggacagtga
     9421 tggacggtac agcacgtttt ctttctaacc atcaaatcga agtgacgaat aatggtgaaa
     9481 aagtgattgt tgaaggcgaa cggattttca tcaatactgg ttctgaacca atcattttac
     9541 caatcaaagg attgaataca agtcgctact tgattgattc tacgcaagcg atggatcagg
     9601 aacaattacc ggaaaaatta gtgatcatcg gggctggata tattggcttg gaatttgcat
     9661 caatgttcaa tgaatacgga tctaaagttg tcgttcttga tgcccattct gaatttcttc
     9721 cacgtgaaga tgaagatatc gcgcaaatga ttttagaaga catgacaaat gcgggaatcg
     9781 aattccatct aggtgtatct gtagatcaag tagcagacca agaatcaaca gctgccgtca
     9841 cctttacaga agatggccaa gaagtaacaa tccaagcgtc aaaagttttg gctgcaactg
     9901 gaagaaaacc aagtacagca tcacttggat tagaaaatac agatgttaaa ctaacagatc
     9961 gcggagcaat cgctgtggat gaataccttc gtacagatgc agacaacatt tgggcgattg
    10021 gagacgtcaa aggcggattg cagttcactt atatctctct agatgattat cgaatcatta
    10081 tggatcagtt gaagggcgag aataaacgaa caacgaataa ccggaaagct gtgccttaca
    10141 gtgtatttat cactccgact ttatccaatg taggtttaac agaaaaacaa gccaaagcac
    10201 aaggcgtcaa ttacaaatta ttcaagtata tgacaagcgg tgtccctaaa gcacaagtat
    10261 tagaagatcc taaaggtgta ttcaaagttt tagtcgaccc tgaaactgac ttgattctag
    10321 gggcaagtat ctatgcggaa gattctcatg aagtcatcaa cttgatttcg ctagctatga
    10381 atggaaaact tccttatacg ttattgagag atcaaatcta ttctcatccg acaatgagtg
    10441 aagctctaaa tgatgtatta aaataaataa cgaatggaga atccaccatg ttcaataata
    10501 aaaatacagc agtagttctc actgacccgc aagtcgagtt cttgaaacca gcaggcggtg
    10561 gctttggttt gacaaaagat atcttagaaa aatacaatac gatcgaaaat atgattgaaa
    10621 tggtaaaaca agcaaaagaa aaaggataca aactctttat ttctcctcat tatttttacc
    10681 cacatgacca taaatggcaa tttggcggtg caggagaaca tatgatgctt gagcagcaga
    10741 tgtttgctag aaaaagtcaa tatgaatcag tggaagaagg ttcaggagcc gattttatcg
    10801 aagaattgaa accttatttg gatgaaaata cgatcatcag ctctcctcat aaaatctttg
    10861 gaccagaaag caatgatttg gcgctccaat tgagaaaaaa tggaatcgac acagtaattc
    10921 ttggtggaat gaacggaaac ttgtgtagcg attcccatat gcgtgaattg gtagagagtg
    10981 gattcaatgt tgtggtagcg acagatgcaa ttggtgctcc tggccaagaa gcaatggatg
    11041 cagcgttgac gaactttggc tttattgcag atcaagtgat gacaacagag gaagtattag
    11101 agcaactatc ataagatagg aattgctttt gtcctcgcag ttcttgaatc aatgaacatc
    11161 tagctatatg agaacagttt tatagtgagg gctgtgtcag ataatttggc acagtctttt
    11221 ttaaacgatt aagaagaaaa aatgatgaat ttactgtata atataggact atgagaaatg
    11281 aaaaaggaga atatcattga aaacaaagga aaagatcttg gaagcgacca aagatcttat
    11341 ttataaaaaa ggctatcatg aaacgtcaat ccgagatatt ctagcagctt ctgatacagg
    11401 aaaaggtcaa ttttattatt acttttcttc aaaaaaagaa gtttgtctag cggtgatcca
    11461 tgcgcatatg gacgtatggc agacagaatt gttcgaagac atcttaaatg ctggaaaaca
    11521 gccagaagaa gcattagaag aaatgttgga ctggattttc gctgatcatg agacacagat
    11581 cacttatcat ggctgtcctg taggtaattt gattattgag ttgagtacag tggatgaaga
    11641 ataccgtctg ttgctactag aattagtcaa tcaatgggtc aagtatacga aacaaaatat
    11701 cctggcactt tctgaagaaa aactgtccga tgaagaagca gaccgtcaag ccaaagcgtt
    11761 gattgctgaa atccagggaa gtatcttatt gttgaaagtc acacaggatc tatcactact
    11821 taggcagaat tttcaattgg tcaaagaaag attgttaact tcgcaaaagg ttctttaata
    11881 at
//
LOCUS       DACEND010000087        11569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEND010000087 DACEND010000000
VERSION     DACEND010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.089"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(157..2055)
                     /locus_tag="IW746_001917"
     CDS             complement(157..2055)
                     /locus_tag="IW746_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1078815.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2216..3478)
                     /locus_tag="IW746_001918"
     CDS             complement(2216..3478)
                     /locus_tag="IW746_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078816.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3471..4370)
                     /locus_tag="IW746_001919"
     CDS             complement(3471..4370)
                     /locus_tag="IW746_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078817.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4515..6293)
                     /gene="uvrC"
                     /locus_tag="IW746_001920"
     CDS             complement(4515..6293)
                     /gene="uvrC"
                     /locus_tag="IW746_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1078818.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6475..6789)
                     /gene="trxA"
                     /locus_tag="IW746_001921"
     CDS             complement(6475..6789)
                     /gene="trxA"
                     /locus_tag="IW746_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1078819.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6876..9236)
                     /locus_tag="IW746_001922"
     CDS             complement(6876..9236)
                     /locus_tag="IW746_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1078820.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9351..9899)
                     /locus_tag="IW746_001923"
     CDS             complement(9351..9899)
                     /locus_tag="IW746_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1078821.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9957..10394)
                     /gene="zapA"
                     /locus_tag="IW746_001924"
     CDS             complement(9957..10394)
                     /gene="zapA"
                     /locus_tag="IW746_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1078822.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10596..11513
                     /locus_tag="IW746_001925"
     CDS             10596..11513
                     /locus_tag="IW746_001925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1078823.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT         3267 a         2456 c         1964 g         3882 t
ORIGIN      
        1 gtgaaatcag cgccctgacc tgagtattag agaaataaac agaaaaatag cttctaatat
       61 tttaccgttt tttgagctaa ttccgaagga gactgcgaca cgccttttgt tgcagtcgct
      121 ttttagtcta ttgagcaatg ttggctagca tttttctcaa tcgtcctttc gataatccaa
      181 cagcctttgg agttgagtaa tctgttgctg taattccatt ccttttgtcg tattccgatt
      241 aagtttgcgt gggagctgct gctcgatcac tttttttaca ttcgtttgat cagatttttt
      301 tgcaaattgt tgttcaatag gcaagaacgg ataatgtgtc acgatttgga aaccgtaaga
      361 attgcatagt aatgagtagc cagcaatccc ggttgttttt tgataagctt tggatagacc
      421 tccgtcaata acaaatgctt ttccgtttgc tttgatcggt gactctcctt tttttacttt
      481 tactggcgtg tgtccattga tgattgctga accttcttct tttgcatcga attctgccaa
      541 aattttttca ctgaaccact cttcatctcg caagtgataa tagggattgc ttttttcgac
      601 gtgcgtatga ggctctgtga gaaaatagcg ctcaaaagtt gccattttac tcttgccgaa
      661 taaaggcgaa aatggcccgc tccacgcata ccaaatcaaa tcggtcgcca aatcttcatt
      721 tgcagtcaaa tcttttgcgc tttcagttat ctgtttttca aaaaatccca gcagttcttt
      781 tccgcgataa gattggtcta attcaaatgc taagagctgc cccttttcat ctacaggtat
      841 acagccatgg aacagtacat gctgattgtt cgtgagatac attccccctt tttcgatcaa
      901 aaacgtcatg tgtttttgca ttcttaacga gtgctggaat gaatacatca aactatcgat
      961 cactttttgt tcctcttcca agaatttttt aggatcatct ggctggatcg tttgaaaaca
     1021 agtccctttc aactcataga gctgaccttc taaaacgatc gttgattcct catagttgat
     1081 tttttccaac aatttacgat cgtccatttt aaattcaggt cgtcgattca acaactgatg
     1141 ttctgatttg aattggagga tagaaagtgc ttgatggatc ttttctaatt cttcttgctc
     1201 tctttcagat agatctttcc tgtttttagg ggtaaatact ggattcttct gataggtttg
     1261 atgggcaaaa gaaaataatg gacgtaaatt taatccatat tctttctcta attcaaataa
     1321 atagttgtag cgtgcggcaa tccgtaaaag cgtcaataaa catgcctcag agccgcaata
     1381 cgcaccaatc cacaaaatat catgattccc ccactggata tctacagagg aaaaatccat
     1441 gattcgatcc attactttgg ctgcttgagt tcctcgatca aaaatatcac ccacgatatg
     1501 caaatgatcg atgacagatt tttggatcgt ctgcgcaagt tttgttaaaa atttttcttc
     1561 ttccttcatc aagacaagtt gttcaatgat tttttttaca tagtcttttt tcccgttgat
     1621 acgatcatcg atatacaaga gttcctcaat gatatagcta tattctttcg gcaatccttt
     1681 tcgaactttt gatcgagtgt atttgactgt cgtaaaccgt aaaagatcga tcaaacgatg
     1741 gatcagctgg gatttatctt gttttttgta ccaatcagtc gtcaacacat attctggata
     1801 agccgtaaaa atagtgaatt ggctgatctc cgtttccgtc atttgttcag agaaaaggtc
     1861 tttgatcttt tctttaagat ttcctgaacc gatacgcaga atatgatcaa aagcaggaaa
     1921 ttcgccatgt atatcgctca aaaataactc tgttcctttt ggaagctgca aaattgcttc
     1981 gagattgatg atttccgtga tgacatcttc cgttgtaggg aacttctctg aaagtaaatc
     2041 taaatagcgg tccattaaaa cgcctccaaa aattggtata ctcaacatta taaagttgtt
     2101 atgctctatt ttcaatgaat atatatccaa tataataaaa aaatagaaat aggtctatac
     2161 aaaaaagaag ctgggaacaa taaatgtcca gcttccttct atatttttct atattttatg
     2221 ctttttttcg ttcatttctc ataattgaaa tcgattgttt caatgaactc cataatccgc
     2281 cttcgctata cacgagtaca ttcgatttgt acatgtttgc agacaagatc atcaataaga
     2341 atgttacggc tacaagtacc aacaaggaaa tcaatgctcc acccataccg actgtctcat
     2401 ttgctaagcg aaccggcata atatatgaag ataagaatgg aatatacgaa gtgatccgaa
     2461 taacgatatt gtttggatct gaagctccta gtatcaatcc aagcatatat ccgcctagag
     2521 atagataagt cactggcaaa attgcttttg ctgtatcttc tgctttgttg accaaagagc
     2581 cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa gatcatgaat agaagagaat
     2641 accacaagaa tggtccaatc aagttatcca atgagatacc gtctaatata tttttgacca
     2701 tttccatatc tttgaattga ctgtatccta taccaaaaat aataccgtat aatgccattt
     2761 gcgttaatgc aacaagtaaa acacctgtta gttttccgta aaaatgcttt tgtgcagtcg
     2821 tactggacag gatcacttcc atgatccgcg tgcctttttc agaagcgatc tcttgtgcaa
     2881 tgatctgcgc atacgtcaaa ataataatga acaagacaat cgttgctaca taacttacga
     2941 catattggac tgtcgagtta tcttctccaa ctgtcatttt tccttttgaa tcaaaattga
     3001 ctttttgtcg agtcaaactt gcttgttggc tcaagctagc tacttcttct ggtgaaatgc
     3061 ctaattgact tgctcgaagc atcgattgca agccagtcag ttgttgttgt atcagtagtt
     3121 gtgtactttg tcctaatgaa ttttcgctat aaagcatccc atttatctta tcattctcag
     3181 ttgagactac taaatacgca tcgatttttt cttccgaaag ctctttttgt gctttttctt
     3241 cagaatcaac tgctttgaac tcaaaatcat ctgttttgac ttggctcatt tgctgactga
     3301 tttgctcatt atcagccaat acgccgattt tattgacatc actattttgt tgagcaaagt
     3361 ttcctgctac ataaatgata cccagcatga taaaagggac taatatcatg atcaagaaag
     3421 aaatcgactt aacatttttt aaatagacat ctttggtaat aatccaaaac ttacgcatcg
     3481 atttcacctg ctttcaattt gaaaatctct tccaaagttg gcggttgctg gttaaacata
     3541 ggaatgtagc cgaactgagt cgcacgagta aagatttctt tcccagcttc aggatcttct
     3601 aaggtaatct ctaacgtatt gtcttcatga aggatcactt ttttcacgcc ttcgatttct
     3661 tctacttcct gttggcttaa accagattcc aagaataact tcgtacggcc aaatgattca
     3721 cgaatctcat gtacttttcc attcaaaacc atttttccgt ttcgcaacat gatcaaatga
     3781 tcgcagattt tttctacatt atccatattg tggctagaaa aaatgacaca tgaaccatgt
     3841 tttttcaatt ccaatattcc atcttttagc aattcagcat ttaccggatc taacccgctg
     3901 aatggctcat ccaaaatgat caatttaggt tcatggatta acgtggcgat cagctggact
     3961 ttttgctgat ttcctttaga caaagatttt actttatccg tctttttccc ttttacctga
     4021 aatttttcca tccaaaaatc gatttttggt tcaatctctt ttcttgattt ccctctaagt
     4081 tctgcaaaat aaagcagttg gtcttgaatc gttactttcg gataaagtcc ccgttcctcc
     4141 ggaagataac cgatgacatc ataatctttc tccccaagat gatggccatt ccataacact
     4201 tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa aagtcgttgt cttcccagcc
     4261 ccgttttggc caattagccc catgattttt ccatcgggga tttggaaaga gacatgatcg
     4321 acagctgtgt agttcccaaa agttttaaca agatccttga cttctaacat ttcttcactc
     4381 cttcttcaag aaatcacata tatccttaga caccagacgt aagcgattta ttatttacca
     4441 acaaaacgta aacgctcttt ttgttgtaca taaaatgata ggacacttta ctttctttgt
     4501 aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc gacatttttc
     4561 ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt caacgatttg
     4621 aactctttaa gcagcatttt ttttcgtttt ggtcctaacc cttcaatttc atctaatcgg
     4681 gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa tcgatggact
     4741 tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag cggtactaca
     4801 gccaaatcta taccgaatag caactcattt gttttatgtt tatcgttttt tgccatgcca
     4861 gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac atctacttgt
     4921 ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg aatcactcgt
     4981 gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc gttgactgtt
     5041 tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa tacgaccatc
     5101 gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat acgtattggg
     5161 gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg ttcctgtttt
     5221 ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttatt cgccaattca
     5281 actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa caaagcttct
     5341 acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa atgctcattt
     5401 tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt atagaaagga
     5461 aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac ttggacacac
     5521 atccatcctt tgtcaatcgc ataaccaaat acatctcgat ccagtaaatc agtatttgtc
     5581 attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta ttctgcagct
     5641 ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc tgactcgatt
     5701 ttttcgtgtc ctccgtttaa aaactttttc acttcatcaa cgattttcgt atagactttc
     5761 ggatctacgt cgaaataata aggacacaga cattgtccca aatgatagta aagacaaggc
     5821 tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag gatcttcttt
     5881 gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt gtcttttagt
     5941 actttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag gaaaggatag
     6001 gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat cagattgatc
     6061 tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc ttcgatttca
     6121 ctaacaagcc gttccgtttt cgtattatgg ctgccagtaa aatatgagcg gacacgattt
     6181 tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt catcaagtaa
     6241 cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt catctggtac
     6301 tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca atccagcctg
     6361 acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac cgcaaaaaaa
     6421 ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa gtttttataa
     6481 atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag ctttttctac
     6541 tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga attgttgagg
     6601 agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt catcatattc
     6661 ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc aagttgccca
     6721 aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat ctgtaattac
     6781 ttgtgacata tcaaagacct ccatttttaa aatctatgat tagtatagca tttctatact
     6841 tggatagcga accatctgct tacctttagt aagctttact taaatttaac aattgtcgca
     6901 ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg atggttttta
     6961 agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac aatagtcact
     7021 tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact tagcgcttct
     7081 tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga ttcagatcgt
     7141 acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt cagctcgtct
     7201 tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc gtttttacgt
     7261 aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg tttcaacgtt
     7321 ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc ttgatgtaga
     7381 tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc ttgcccaatc
     7441 tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc cgcctctgac
     7501 acgacttcgt ttgctttttt ctttgctttt tccatttctt tttcacgttc ttcaaagaaa
     7561 tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac aaaatgacgc
     7621 atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt gatcatttcg
     7681 tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac ttctgtatca
     7741 agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc aatcagcaaa
     7801 cgatacgttg gacttagtgt atccacatcg aactccatgc tggcattaat cgtatttgcc
     7861 cgattgtatc cataaacttt caattcagga taatgcgttg ttgccatcac ataagcagac
     7921 tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg cggatctgtt
     7981 cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga taaaatatct
     8041 acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga ctgttcgtct
     8101 cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc tgcaggaatc
     8161 ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt gatcgttttc
     8221 ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat tgtgatgtca
     8281 ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa gacgacatga
     8341 ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg tgcttttgca
     8401 ttcatcagat ccattttacc aatcacataa gcattgtgga tgatttcttg acgatgggga
     8461 acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc agcgatttgg
     8521 tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa taaagtctgt
     8581 cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc ttgtttgact
     8641 gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata ctttgcattt
     8701 ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg ccgaatgttc
     8761 tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc ttcatcgatt
     8821 gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc ccacatatac
     8881 aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt caattcgctt
     8941 gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc tgctccgatc
     9001 tcgatccgtt tcatatgggg acggatattt tcaagtttag gtatagggat cccgcctcgc
     9061 agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat cgtttgtttc
     9121 tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac tacaaattgt
     9181 ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt attcatttta
     9241 tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg cagccttctt
     9301 aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc ttaattcaaa
     9361 atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct ctcaacgata
     9421 aaacgagcga aactatgctc gccgaattga ttttggacca catctaatgg aacaaaggca
     9481 agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag gatgcctcct
     9541 gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac aaaaaccccc
     9601 acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt tcctgcataa
     9661 aaagcttcat ctaggtgaaa agcaaatacc tgatcaaaaa atgctaactt tgatgttggg
     9721 gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt gtaaaaatgc
     9781 tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa agcaaacccg
     9841 cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa actcagcatg
     9901 ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa aggatcttaa
     9961 ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg ttgtgcttcc
    10021 acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc ccgagcttcg
    10081 ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt gatcattttt
    10141 ttctttaatt ctgctgtttc ttcttccagc tccaagattc tttcttgttt cttcaattga
    10201 tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat ttgtggtgat
    10261 aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc catatgatgc
    10321 ttggtttccc gtccgataat agtatatgtt tggttggcaa tcactgcttt gtagcgtgtt
    10381 ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt tagtgtacca
    10441 tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac tattatatga
    10501 taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt cgtgagttgc
    10561 ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt atcaaagcaa
    10621 caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta aataaacaga
    10681 ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct tatacatccg
    10741 gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg ggggaggcac
    10801 aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat atcagtcaat
    10861 tatccgtttt aggtagtgat gaggtcggaa atggcagcta ttttggtccg ctaacagtat
    10921 gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt gttagagatt
    10981 caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa aaaaccatcc
    11041 cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca aaatacaatg
    11101 ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta aaaaaaatcg
    11161 agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca aattttaaaa
    11221 aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt actaaaggag
    11281 aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc tttttggaag
    11341 agttacacaa agcatcagct gaagctggaa tcacccttcc atcaggagct ggaacgaaat
    11401 ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt gaaaaatatg
    11461 ccaaacttca ttttgccaat acagaaaaag ctttgaagct gctaaaaaaa taatggaact
    11521 gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttctc
//
LOCUS       DACEND010000088        10993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEND010000088 DACEND010000000
VERSION     DACEND010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.090"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW746_001926"
     tRNA            complement(77..149)
                     /locus_tag="IW746_001926"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW746_001927"
     CDS             530..1099
                     /locus_tag="IW746_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1078824.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW746_001928"
     CDS             1086..1946
                     /locus_tag="IW746_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1078825.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW746_001929"
     CDS             2136..2840
                     /locus_tag="IW746_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078826.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW746_001930"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW746_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1078827.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW746_001931"
     CDS             4677..5993
                     /locus_tag="IW746_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078828.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW746_001932"
     CDS             5994..6863
                     /locus_tag="IW746_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078829.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW746_001933"
     CDS             6918..7727
                     /locus_tag="IW746_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1078830.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW746_001934"
     CDS             complement(7830..8459)
                     /locus_tag="IW746_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1078831.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW746_001935"
     CDS             8629..9921
                     /locus_tag="IW746_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078832.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW746_001936"
     CDS             complement(9997..10659)
                     /locus_tag="IW746_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1078833.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..>10993
                     /locus_tag="IW746_001937"
     CDS             10884..>10993
                     /locus_tag="IW746_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1078834.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTG"
BASE COUNT         3624 a         1909 c         2350 g         3110 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgg
//
LOCUS       DACEND010000089        10846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEND010000089 DACEND010000000
VERSION     DACEND010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.091"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..2161)
                     /locus_tag="IW746_001938"
     CDS             complement(86..2161)
                     /locus_tag="IW746_001938"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1078835.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2163..3080)
                     /gene="glyQ"
                     /locus_tag="IW746_001939"
     CDS             complement(2163..3080)
                     /gene="glyQ"
                     /locus_tag="IW746_001939"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1078836.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3464..4276)
                     /gene="recO"
                     /locus_tag="IW746_001940"
     CDS             complement(3464..4276)
                     /gene="recO"
                     /locus_tag="IW746_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1078837.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4419..5318)
                     /locus_tag="IW746_001941"
     CDS             complement(4419..5318)
                     /locus_tag="IW746_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1078838.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5333..5734)
                     /locus_tag="IW746_001942"
     CDS             complement(5333..5734)
                     /locus_tag="IW746_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1078839.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="IW746_001943"
     CDS             complement(5712..6188)
                     /gene="ybeY"
                     /locus_tag="IW746_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1078840.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6205..8397)
                     /locus_tag="IW746_001944"
     CDS             complement(6205..8397)
                     /locus_tag="IW746_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1078841.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8421..9392)
                     /locus_tag="IW746_001945"
     CDS             complement(8421..9392)
                     /locus_tag="IW746_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1078842.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10097..>10846
                     /locus_tag="IW746_001946"
     CDS             10097..>10846
                     /locus_tag="IW746_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1078843.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFG"
BASE COUNT         2935 a         2289 c         1974 g         3648 t
ORIGIN      
        1 cggcgagtta tttatattta taacaaataa accagagaaa tagcctattt tgtgcctgtt
       61 ctctggttta tttacatatt agaaattatt ttgtgatgat ttgatctaaa cttgctagtg
      121 acaaagccat ggcagcaatt tgacttaatt gcgttaatcg gttcttacgt actgctggtt
      181 cttctgccat gaccatcgtc tgatcaaaat aattttcgat cagtgggcga aggcttacaa
      241 gctgatcata attttcgttg atcgtttggt cagcaaagct tgttttcatt acctctactg
      301 ctttgtgcag ttctttctcc gcttcgtttt cgaacagttc agggtctact tctgattgtt
      361 tttcaagctc cgcttttttc gctaagttca cgacacgtgt caaagcttcc atcgatggtt
      421 tgaagtcttc atctgcaaga tgctgcttga acaattgtgc tgctgcaaat acttttgtca
      481 gatctttttg ttctgcgttc aaaacagctt cgatgacatc atgacggata ttcttcgttg
      541 tcaataattg gcgcagacgg cctttgataa agtcgatcac ctgttgttgt cccgctgaca
      601 agccaatgcc aaaacgatcc gtatctttac tgataacttc actaatcgtt tcttgcagaa
      661 cagacaaagg aaaggcccat tttttcgatt cgatgattcg aacgacacca tatgcttgac
      721 ggcgcaaagc atatggatcg tttgagccac ttgggatcat cccaactgca aagaaagaaa
      781 agatactatc taatttatca gcaatcgcta aaacagaacc tactgtagtc tcaggtaaat
      841 ctccttcact ggatataggc atgtaatgtt cacggatcgc tgttgctacg gctggtttct
      901 cgcctttaag cagagcatat ttttctccca tgatcccttg tagttcaggg aattctccta
      961 ccatgttggt tactagatca aatttataga tttcagcagc gcgttgcaga tccatcaatt
     1021 cttcctcaga cagtcctact tgttcgccta tgatgcgagc gatgacttgt acccgttgca
     1081 ttttctcata gatgctaccg attttttcat ggaaagtaac agtttttaat ttttcaacgt
     1141 attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct tctaaacgag
     1201 cagtcaatac tttttgattg cctcgtacca cattttccaa ttttacccga tcgccattac
     1261 gagcagaaat aaaatgagga agcaacatac cttgatcgtt ccgtacttcg aaataacgtt
     1321 gatgttcttt catagaagtg actagtactt cttcaggaac agatagatat ttttcttcga
     1381 agtcgccaga aaagacagtt ggatattcga ctagattgtt gacttcttct agtaaatctt
     1441 catctagatc aatgatccag tgatcttttt ctgctaattc gtttgcttgt gtgatgatca
     1501 tttcttttct tttttgcggt gaagcaataa caaattgttc atttaatttt tcttcgtatt
     1561 cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctctt gaagctctgt
     1621 ctgttttgat atccaagatc tcaaacggga ttacttcgtc atctaacaat gccacgatcc
     1681 agtgaatagg acggatatat tcaaaatcgt attctgccca atgcatcgtt actgggaaag
     1741 tgaggctttt aatgacatct gctaaacctg tcaatacttc ttttgcttct ttgccgtgag
     1801 tgtatttcgt tacatagaca tattccacgc cttttaattc tctaaaagtg atagcatctg
     1861 tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt ccatctgcgt
     1921 ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtctgct tgacgttctg
     1981 gaatgtttgt tactttgatt gctaaacggc gcggtgtaga aaatgtttca atcgtatcat
     2041 aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt ttcatgcttg
     2101 gtgtgacaac atgagcaggc atttcttcca gcccaatttc aagtaataag tcttttgcca
     2161 tcttattcag cctccttcac gttttctgga tttgctgtgt ttttattcaa taaagggaat
     2221 cctaattttt cgcgttctgc cacaaagatt ttggctacag aacgagccat gttgcggata
     2281 cgggaaagat aacctgcacg ctccgttacg gatactgcac cgcgggcatc taataaattg
     2341 aatgtatggc tgcatttcaa aatataatca tacgctggat gaacaagatt ttcatcgata
     2401 cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc ttggtcgctt
     2461 acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc gccatatttc
     2521 acgccatcag cccattctaa gtcatagaca ctttcaactt cttgaatata tgaagcaaga
     2581 cgttctagtc cataagtaat ctcagaagta actggtttac aaggtagtcc tccgacttgc
     2641 tggaagtacg tgaattgtgt aatctccatc ccatcaagcc atacttccca acccacaccg
     2701 gcacaaccca tcgatgggtt ctcccagttg tcttcaacaa aacgaatatc gtgttctaat
     2761 ggatcaattc ctaaaaaacg caagctttct aaataaagct cttggatatt ttcaggtgaa
     2821 ggtttcatta cgacttgaaa ttgatgatgc tgatacaaac ggtttgggtt ttctccataa
     2881 cgcccgtcag ctggtcggcg ggatggctct acataagcag cattccatgg ttccggtccg
     2941 attgcccgta agaatgtata tgggctcatc gtacctgctc ctttttctgt atcataagct
     3001 tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat catatcttgg
     3061 acggtcaatt ttttactcat ctgttttgcc cctttcaatt tttaagcgtt gctgtcaata
     3121 agaatacagg aaagaaaacg acaaaaagtc cctatgtctg attacagaca tagggacgaa
     3181 taatttcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt tatcgtactc
     3241 gcaagtgcct cttcaaaaat cgtttgggtc cggcttccac tctcccgaac tcgctaaacc
     3301 acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt cttgtttata
     3361 taatagatcc tctcagtgaa cgatctattt aatctacggt taataataga cggaagtccc
     3421 tgtatttgtc aaatagtttt gatttttttg ttgtatcttt catttattct tctttttcag
     3481 gtatctcttt ttcaggcata tttttctctg gcatctttaa tacgtgtgcc caagacttca
     3541 tttgatcaat gaactttttg ctttttagat gaataccgac atattcatca tacaactggt
     3601 caatgaggtc acggattgcc tgcttcgttt cttctttcaa gtggattcca ctgatcttat
     3661 cataagaaat agccgaaaac attcgaacga aataaacagc gcgaggatat gcatgataac
     3721 ggtgttcgtc cattggccag tgtttctcgc atatcacgcc gccatatttg gaagaatagt
     3781 caaattttcc ttttgtttcc ccgcatacga cacagtgcgt ccaaattggc gcgattccga
     3841 agcgttgcag tatttgaact tcaaagatat tcgtgatgat ttctgcatca tctccttggt
     3901 ccatccgctg cagagcttgt tgcgtaaatt ggaataagtg cgggtcatat tgatgatctt
     3961 cgattgctgc atctactaga tttagcaaat acgtaccata accgctcaaa aagatatctt
     4021 cttggatgtt ccgaaacgca tggacgtctt tgacactgct taaaaacgac aaaccttcac
     4081 ttagaaaatt accgatatag actgcttcgg taaaggggag caaagccgga gctaatggct
     4141 gattttgccg atggattcct ttgatataaa acatcatttt gccagaagat tccgtaaaga
     4201 ttttgactaa tttatctttt tctttgtaat tacggctaga taaaatcaag cctttcgatt
     4261 ctgcaagctg gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg
     4321 atccagatta tttatgttac gatctttcgc tgtttttctc aacgtacgga tttttatcca
     4381 gtttatttct atacctaacc tataagaagt ccagaatatc agtactcgtc ttttcggtag
     4441 ccgaagtctt gcagatagac ttttttgtct cgccagtctt tttggacttt gacccaaagt
     4501 tctagaaata ctttgtctcc taaaagattt tcgatatcct ttcttgcttt cgttcctact
     4561 tgcttcagca tttttccacc tttgccgata atgattcctt tttggctatc tctttcaacg
     4621 atgatcgtgg cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg
     4681 actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat
     4741 tcagaaacga tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg
     4801 ccctctggca tttcactgac taatacatcc attaagcgtt cgacattgtt tccttctgta
     4861 gcagagatcg gaatcacttg tgcaaaaggc atctgcgtcg aataatcttc gatgattccc
     4921 agcaactcgt ctggatggat cttatcgatt ttattgataa tcagataaac tggagaatgc
     4981 acgtttttta gacgttcaat aataaaatcg tctccccggc cgcgtttttg atcagcactg
     5041 atcataaata agactgcatc cacttctttc aacgcagtgt aagctgcttc taccataaaa
     5101 tctcctagac gatgctttgg tttatggatt cctggtgtat cgataaaaat cagctgagct
     5161 tctggtgtag tatatactcc ttggatttta tttctagttg tctgtgcctt gtcactcata
     5221 atggcaattt tttgacccac gattcggttc aataatgtgg attttcctac attcgggcgg
     5281 ccgacgattg cgacaaaccc tgatttatgt tctggcatat acgttctcct ttttaattaa
     5341 acaacaaatg atatatttta gggacaaata aaatcaaccc aatgatgaca gcaaaacaag
     5401 cggtcactaa aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat
     5461 gaaaatccgt aaacatgtcc actacatttt caaaaacagt attgatgatc tctactatcc
     5521 aaacgagaaa aacagctaaa agcagccata accactctat ccgatcaatc tggaaaatca
     5581 cgcccataaa taaagcaagg ataccgaacg caacatgttt cctcatattg cgttcttcct
     5641 caaaaacggt tttgactcct tggacagcaa attccacgga catcaggaaa tgtttgtttt
     5701 tctctacgtt tttatctttt gagtccatag gcatccaaga tctctttctg taaaccaaac
     5761 atttctttct catcttctgg tgtcatatgg tcatacccgt tgatatgcaa gaatccatgt
     5821 accgctaaaa agccaagttc ccgatcatat gaatggccgt aagcttctgc ttgttcagca
     5881 gcacgttcaa tagagatcat gatgtctcct agtacacgag gcaatggatt ctctccatct
     5941 tcaaaaatca ctgggatttc gtcttcgcct tcttcttcca gagcaaaact gatgacgtct
     6001 gtcggtgcat cttttccacg gtaatcacga ttgattactt ggattgccgc attgtccatg
     6061 aaagtaacag acatttctgt ttcctctggc agctttaaat aagatgctgc aaattgcagc
     6121 aagtcatcga tttctttcat tttctcttct gagacttggt ttgtttcatc cataaaggta
     6181 atatccatta tacatcgctt ctttctattc ttcttgttct tcctttatct tctcaacttc
     6241 tgttttcatt ttaggatatt tgatccgaga atggaaggtg ctacttaaac cgctgatcaa
     6301 tgatttttcc acttttcgga tttctttcaa ggtcaatcca ctatcgtcta attgaccatc
     6361 ggaaatacgc tcttcgataa gattatggac aaaactttcg attttttcgc tcgttggatg
     6421 atccattgcc cgtactgcag cctcacaact gtcagcaata ttgacgatcc ctgcttcacg
     6481 actttgcggt tttggtccag gataacggaa ttgctcttct gttacttccg gattgcgttc
     6541 tttcgctttg atgtagaaat aacgcattag cgtggttcca tgatgctgtt tgcaaatatc
     6601 gatcaccatt tgcggcatat tataatcttc caatattttg gctccatctg tcacatgtcc
     6661 aaagataata tgtttactgt cttctggcaa taagaaattg tgcgggtttt ccgctcctga
     6721 aggcaagttc tcaacgaaaa agctagcgtg tttgatcttg ccgatatcat gataatagca
     6781 agctactcga gttagtaaag aacgtccgcc gatttctgct acagcgttag cacttagatt
     6841 agctaccatc atactatgat gatacgtacc tggcgcttct tccagcaact gtttcaataa
     6901 aggatgattt ggattgctga gttcatttaa tacaattaca ctgtcatcat tgaccatcaa
     6961 ttcaatatat gggtgtagac cgatcgtcag caagaatgag aagagtgcac cagtgaaccc
     7021 gcagacaagc attaaccaag tcgttgcatc accaaagctc attccttgat agacgatcaa
     7081 aatgacatcc atcatcacag gaaaaataat gacccacatg gcagcagata atccttgttc
     7141 tgagatccgt ttacgtctga caatcgttgc catgagccca gagaaaacat aagacatcaa
     7201 gataatcgtt agaaagttcg tgccaatcga atcataaaaa atgaaaagtg cggatactgc
     7261 ttggaaaata ccagccatga tccctgctct tcgattcaag aaaaagctta aaacaagtgg
     7321 tgcaaaagca gcgggataaa acagcgggat ataagtagct tgttccgttt ggaataattg
     7381 gaaaaatttc atcagcacta tgctgactga catagctgtc acatagaaaa gaacgtattt
     7441 tgttctttct cctgtatttt ggaattgaag tgaattataa atcagtacgc cgatttgaag
     7501 caaaacagct aaaaccatgg caattagcgg aaaaatcgaa gttgtttgat tagtcagtcc
     7561 tagtaattcc aattttttca tagctgaagc atcaatttga ttaccttcac gaacaatgat
     7621 ttctccttgg aagatcatca ctggttgtac agcttctcgt gcggattgtt tcaattcttc
     7681 tgtctttttc tcattcaata cgtcattcac tacgatacct tgatccacta agtagcgaat
     7741 catttgttgt tgtgctggtg tgacattcaa atattggatt tgctcttctg cttcttgttt
     7801 aaaagcttcc aattcacttt cacgaacttg tttgctcatc tgttcgtcaa tgagcttcag
     7861 actctcatcc ctcacagtat gaatctcagt ttcagtcatt tcaaatatcg tcgaataaaa
     7921 attgttcggt agtttttgat aaaacacaac attttctgca ttcacatttt caaaattttt
     7981 cttcagcgcg gcgatccgct cttctgtagt aggctttggg atattttcat cttcctttgc
     8041 cttatcaatc ttttcgtcat aagatttgtt gatggcatca ttcgtcttat cgatcaattc
     8101 aaatagttgc ttgatccgat tgttctggtc atctgcaaga tctttttggt aagtgtattc
     8161 tggagtgact gcttctgctg ccagttttct tttttgttcg gtttcttccg tgttctcgat
     8221 cgttttgttc gcacggatac tttcttctgc tacttggcct tctctgaaat cattggcctt
     8281 ttgtttgaca ctactaaatg taacgccgca gataatgata aaaaacaaga acagcaggaa
     8341 aggcaatgct gcctttccaa gtttttcttg tagtttctgc agtgttttat tcaacatgtg
     8401 cttatcctcc taagcaaagc ttattcttga tggagatctg ccttttcata agctttgatg
     8461 atttcggcaa ctacagggtg ccgaacaacg tcacttgctt caaaattaac aaaatcgatt
     8521 ttcttgattt gctttaatgt tcgttctgca tgaacaagac cacttgttgt ccctcgaggc
     8581 agatcaatct gactcgtatc tccattgaca atcatttttg attgataacc aagacgtgtc
     8641 aggaacattt tcatctgagc aactgtcgtg ttctgtgctt catccaaaat aacgaatgca
     8701 tcatccaatg tccgcccccg catgtaagct aaaggagcaa tttcgatcac gcctcgttcc
     8761 atcaatcggt tcgtatgatc taatccaaaa atttggtata aagcatcata aacaggacgc
     8821 agataaggat ctactttttc ttttagatcc ccgggaagaa agcctagatt ttcccctgct
     8881 tccacagcag gccgcgtcaa aatgattttt tgcacttcac cttttttcaa ggcagcgatt
     8941 gctaatacta ctgcaagaaa agtttttcct gtcccggctg gtcctacgcc aaatacgata
     9001 tcatgtttgg ctaccgattc gacatatttc ttttggccgc tgttttttac ccgtataggt
     9061 tttccattac ggtctttgac gatctcttcc tcatacatct caacaaaata atctaatgtg
     9121 cctttttgcc ccattttcag tgccgtcacg acatctggtg tacttacatg gattcctcgt
     9181 ttgatcaatt cctgcaatgt ccgaatgact gaggcagcca aggaaatctg ggattgttcg
     9241 ccaatgatct gaatgatttc tccacgtgta tggatggttg tttctgttgt ttcttcaatc
     9301 agttttatgt gcttgtcgtg tgcacctaaa agcatgctga catcatcagc agccgtcaat
     9361 ctaatctcta aagaactaga ctcttccgtc aaaaatagac atcccctttt tttcgattca
     9421 ttctttccta ttttaccata ctcagaagaa actatatagc aaataagaaa aggttctaaa
     9481 agagttgctt aaccataagt accaagaaac gttaaaaaat agctgcttcc attctctaaa
     9541 ggtctcgttt gaataaaaaa agctgatcat gtaagctatg cataagatag aggacaagta
     9601 ttccgtctaa cttagcaagt attctctggg tatttttctt gtttcaaaaa aagaaaacaa
     9661 aatatttgaa atattcttgc atttcaaaaa aaacaggtgt attataacgt caacaaataa
     9721 atatacagca aagcgtcgag gaaaacagta ggctattcag tgatttattc ccagtgagtc
     9781 atgtaagtga gaaatgacaa taaatcgaat agacgaacat catttccgag tcaatgtcga
     9841 aaacgtagat cattgtggct gcctccatta tcaggccagc ggataacttt atcgaaaagc
     9901 gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg taccgttcta gaatctagag
     9961 cccttttccc aagtggaaaa gggctttttt tatattttaa ggtggtggtt ttcatggaat
    10021 ctgatattag tggtatttct agtttctgca acgtttattt tgttactcaa aatgataatt
    10081 attggaggaa aaatttatgg aagaaacaaa aacttattca tggaagcaat tattgatggt
    10141 cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctgatctttc ctgtccactt
    10201 aggtcaaatg gctggatcaa actggatcag cgctggtgta ggatttgcca tctcaggcgc
    10261 tcttttccca ttattagcta tcttagctgt agtagtgaca aaaagtgatg gtctgtatga
    10321 cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttgatccttg ttcatttaac
    10381 gatcggacca ttctttggta caccgcggac agcagcaact gcttttgaaa tggctgctaa
    10441 accctttttg cctgaaaaat atgattcatt aggtatgttt ttattctctg ctcttttctt
    10501 tttacttgct tatttcctta ctgtacatcc aacacgtttg gtcaaatatg tcggaaagta
    10561 tttaaactcc ttgtttcttg cattattagc tatcattttt gtcgtttctt tattccaacc
    10621 catggggaat ctaaaccaat ctgctgcaga ttcttatcag acgaacgctt tgacaaatgg
    10681 cgttttagaa ggatacaaca cggttgatgc agtagcttta cttgctttaa gtgtcacttt
    10741 tgtccatgca gttcgtgatc ttggttttaa agataaaaaa gtcacagaat taacagcaaa
    10801 agctggtagc ctgagcgttg cgttagaagt catgatttat tttggt
//
LOCUS       DACEND010000090        10771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEND010000090 DACEND010000000
VERSION     DACEND010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.092"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(164..388)
                     /locus_tag="IW746_001947"
     CDS             complement(164..388)
                     /locus_tag="IW746_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1078844.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IW746_001948"
     CDS             complement(586..2166)
                     /locus_tag="IW746_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1078845.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IW746_001949"
     CDS             complement(2567..3943)
                     /locus_tag="IW746_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1078846.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IW746_001950"
     CDS             complement(4071..5189)
                     /locus_tag="IW746_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1078847.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IW746_001951"
     CDS             5331..5765
                     /locus_tag="IW746_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1078848.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IW746_001952"
     CDS             complement(5843..6376)
                     /locus_tag="IW746_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1078849.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IW746_001953"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IW746_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1078850.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IW746_001954"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IW746_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1078851.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IW746_001955"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IW746_001955"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1078852.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IW746_001956"
     CDS             complement(10101..10679)
                     /locus_tag="IW746_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1078853.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3151 a         2200 c         1844 g         3576 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa a
//
LOCUS       DACEND010000091        10748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEND010000091 DACEND010000000
VERSION     DACEND010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.093"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..1439
                     /gene="lysA"
                     /locus_tag="IW746_001957"
     CDS             180..1439
                     /gene="lysA"
                     /locus_tag="IW746_001957"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1078854.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1426..2226
                     /gene="dapF"
                     /locus_tag="IW746_001958"
     CDS             1426..2226
                     /gene="dapF"
                     /locus_tag="IW746_001958"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1078855.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2229..3110
                     /locus_tag="IW746_001959"
     CDS             2229..3110
                     /locus_tag="IW746_001959"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1078856.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3107..3823
                     /locus_tag="IW746_001960"
     CDS             3107..3823
                     /locus_tag="IW746_001960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1078857.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3829..4524
                     /gene="dapD"
                     /locus_tag="IW746_001961"
     CDS             3829..4524
                     /gene="dapD"
                     /locus_tag="IW746_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1078858.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4540..5541
                     /locus_tag="IW746_001962"
     CDS             4540..5541
                     /locus_tag="IW746_001962"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1078859.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5538..6899
                     /locus_tag="IW746_001963"
     CDS             5538..6899
                     /locus_tag="IW746_001963"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1078860.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6859..8010
                     /locus_tag="IW746_001964"
     CDS             6859..8010
                     /locus_tag="IW746_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1078861.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7985..9154
                     /locus_tag="IW746_001965"
     CDS             7985..9154
                     /locus_tag="IW746_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1078862.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9329..10708
                     /locus_tag="IW746_001966"
     CDS             9329..10708
                     /locus_tag="IW746_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078863.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3569 a         1875 c         2405 g         2899 t
ORIGIN      
        1 ttctaatagt tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata
       61 tttcgaaata tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa
      121 atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa
      181 tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac
      241 ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct
      301 ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg
      361 agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa
      421 tctataccgc tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca
      481 agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata
      541 atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta
      601 tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc
      661 atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt
      721 tgataaaaga aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa
      781 tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt
      841 ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg
      901 atcagccgat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt
      961 tggaaaaacg aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg
     1021 gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat
     1081 attactttgt agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat
     1141 acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag
     1201 gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat
     1261 cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact
     1321 acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg
     1381 ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa
     1441 tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt
     1501 caattagccg cttctttatg cacaaaagaa aaatatgata tagacggatt gatcgccgta
     1561 aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc
     1621 ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag
     1681 gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct
     1741 ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat
     1801 caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact
     1861 agtttatttt taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc
     1921 tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc
     1981 aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta
     2041 ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact
     2101 gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct
     2161 ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag
     2221 gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat
     2281 cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg
     2341 gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc
     2401 agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg
     2461 gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag
     2521 acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc
     2581 attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta
     2641 gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca
     2701 ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg
     2761 gagaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag
     2821 gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag
     2881 taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg
     2941 aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat
     3001 tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa
     3061 agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact
     3121 gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac
     3181 ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc
     3241 agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt
     3301 atcatatcaa cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga
     3361 aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat
     3421 catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga
     3481 actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct
     3541 gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa
     3601 aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca
     3661 tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa
     3721 tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg
     3781 ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa
     3841 gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat
     3901 gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt
     3961 gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt
     4021 gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa
     4081 attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat
     4141 gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg
     4201 attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt
     4261 gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa
     4321 gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat
     4381 gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt
     4441 ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg
     4501 attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt
     4561 attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat
     4621 tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta
     4681 ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat
     4741 ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt
     4801 cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta
     4861 atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat
     4921 tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag
     4981 cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat
     5041 cttcatcgta cgcaaaatga cgagaaacca gttttgtatc cttataatat tagccaaacg
     5101 gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg
     5161 ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta
     5221 cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt
     5281 acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat
     5341 ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt
     5401 gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat
     5461 aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa
     5521 atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga
     5581 tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc
     5641 aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact
     5701 tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa
     5761 ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag
     5821 atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag
     5881 taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta
     5941 ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag
     6001 cacaggaaat cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg
     6061 gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg
     6121 cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa
     6181 tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta
     6241 gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc
     6301 cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag
     6361 ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac
     6421 gcaaggattt tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta
     6481 tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag
     6541 ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca
     6601 tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag
     6661 ctttgatctc tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt
     6721 tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt
     6781 tgaatctgat catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg
     6841 aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa
     6901 atcgggtttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag
     6961 gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa
     7021 gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc
     7081 tcttttgctt ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg
     7141 atgcttttag ggttgactga ctttataact aaacacattg aggatcttat ttataatgta
     7201 gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca
     7261 ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta
     7321 gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac
     7381 attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg
     7441 gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag
     7501 tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct
     7561 ggagaagtta ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg
     7621 aagaggggat tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc
     7681 tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat
     7741 tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat
     7801 acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat
     7861 gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg
     7921 attcttttta cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta
     7981 aataatgatg aatttatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa
     8041 aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg
     8101 agaaccggat tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga
     8161 agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat
     8221 atttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt
     8281 tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt
     8341 tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga
     8401 attgatcccg atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg
     8461 agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc
     8521 tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata
     8581 tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat
     8641 gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca
     8701 tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact
     8761 aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc
     8821 tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg
     8881 aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg
     8941 tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg
     9001 tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga
     9061 aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg
     9121 caaacggatc aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat
     9181 cgttaatttt gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga
     9241 acgcgtttct tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt
     9301 taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc
     9361 atctcatgga gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga
     9421 tatcacgcta tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta
     9481 tttagaagac aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc
     9541 gcaatccaat gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa
     9601 aacagtaacg gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt
     9661 gattttgagt tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa
     9721 tgtctacttg atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc
     9781 ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc
     9841 aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac
     9901 ttatttagat gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga
     9961 agttttgaca aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa
    10021 aacaagtgaa aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa
    10081 tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga
    10141 atacttgcaa acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc
    10201 tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg
    10261 ttatgtagta aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg
    10321 ttcctctgca ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac
    10381 agcagcaaaa gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa
    10441 atatgtacca gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat
    10501 gacccatcaa ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa
    10561 tgtattggca ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt
    10621 cttcttccag ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc
    10681 gttaggagaa gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa
    10741 aagctgcc
//
LOCUS       DACEND010000092        10452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEND010000092 DACEND010000000
VERSION     DACEND010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.095"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW746_001967"
     CDS             107..970
                     /locus_tag="IW746_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1078864.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW746_001968"
     CDS             complement(1099..2721)
                     /locus_tag="IW746_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078865.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW746_001969"
     CDS             2970..3491
                     /locus_tag="IW746_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078866.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW746_001970"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW746_001970"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1078867.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW746_001971"
     CDS             5572..6390
                     /locus_tag="IW746_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1078868.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW746_001972"
     CDS             complement(6493..6690)
                     /locus_tag="IW746_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078869.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW746_001973"
     CDS             6859..7437
                     /locus_tag="IW746_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1078870.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW746_001974"
     CDS             complement(8482..8985)
                     /locus_tag="IW746_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1078871.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IW746_001975"
     CDS             9344..10369
                     /locus_tag="IW746_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1078872.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         3198 a         1794 c         2046 g         3414 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ct
//
LOCUS       DACEND010000093        10160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEND010000093 DACEND010000000
VERSION     DACEND010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.096"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..193)
                     /locus_tag="IW746_001976"
     CDS             complement(<1..193)
                     /locus_tag="IW746_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1078873.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGE"
     gene            complement(193..570)
                     /locus_tag="IW746_001977"
                     /pseudo
     CDS             complement(193..570)
                     /locus_tag="IW746_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(701..2578)
                     /gene="ltrA"
                     /locus_tag="IW746_001978"
     CDS             complement(701..2578)
                     /gene="ltrA"
                     /locus_tag="IW746_001978"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1078874.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(3266..4960)
                     /locus_tag="IW746_001979"
     CDS             complement(3266..4960)
                     /locus_tag="IW746_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1078875.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(5123..5716)
                     /locus_tag="IW746_001980"
     CDS             complement(5123..5716)
                     /locus_tag="IW746_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078876.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(5717..6049)
                     /locus_tag="IW746_001981"
     CDS             complement(5717..6049)
                     /locus_tag="IW746_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078877.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(6099..8471)
                     /locus_tag="IW746_001982"
     CDS             complement(6099..8471)
                     /locus_tag="IW746_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078878.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            complement(8468..>10160)
                     /locus_tag="IW746_001983"
     CDS             complement(8468..>10160)
                     /locus_tag="IW746_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ1078879.1"
                     /translation="QKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDP
                     DNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIA
                     KMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGYRGFCEI
                     NFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKE
                     AVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQC
                     VKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGE
                     QNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDS
                     DRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNP
                     SWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMHDQIEEEAKENIFIKIV
                     QSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKAVKE"
BASE COUNT         2748 a         2115 c         1615 g         3682 t
ORIGIN      
        1 cttcgccacc actgcttgaa tctgaatcag aattatcaat cgaacccgcc gccagtatta
       61 gaaaaagggt cacgaacaca gtgatcaaaa tagtaatcaa acctgcagaa gtaccaaaca
      121 accacaaaaa taagcgtcgc tttgcacgac gcttaacata ctgcttgatt aaactttcat
      181 tttggttttc cattattttc ctccatcaaa caattttaat tgacgtttgt tcggactaaa
      241 atgaacacga atggtttctt ttgagccaaa attgatcaca gcgtctcctt gattttgttg
      301 aggaatggat tctagttctg agttcgtcat tgatttacca actaatcctt ctatatgacg
      361 taaaatagat tcatccatac gcataaagat cttcgtagaa gaaaattcaa agactttctt
      421 cagtttcgag aagttatcag aagacatatt ttctggcacc atttcttgtg gggattgtgt
      481 agctaaaaca gtcattgctt ctactttacg catttctcgt tgaaagtttg tgacataatt
      541 taccgtttca ataaaatctg gattaatcga tactgatagg taagagtttg atattatctc
      601 cgtcttttcc cccagtccac accgtacgtg cgactttcac cgcatacggc gttccctcgt
      661 ctataaaaga cattctgttt tcccgtccta caaatattta ttaagatatt tcttcgttac
      721 caacttttaa tctcaactca ttgagttttt ctttttgttt tggatttaat tttagatatt
      781 ttagaagttc ctcaatcgtt tcactttgtg tagcgtgtat tacccgatga acctcctcag
      841 taacaatcac taggttttca tagctatcac ttttgctttc atgataaggg ataatatgat
      901 gacaatgcat ttcagataag ataagctcct cacctgttac tgcacatctt cccttttgag
      961 cgatgaattt tgaaattcga ttgtccgcaa attcaatcgt tgctctctta ctaagcactg
     1021 gattgctaca caaccacgat atcttccatt cttctacggt ttgttgcttc ttatgaatgg
     1081 cttccctacc ttcttcggtg tatttattaa tatttctttt tttacacatc ggatgtctgt
     1141 gttgaatata ccctactggt aatattggaa accctcttat atatcgtatt tgtcctgacc
     1201 gtaggtaagg cttgtatcct tcgtactttc cgtattgtcc ttcttttgag attccttgtt
     1261 ctcgtagtcg tatttcaaaa cttggtctga gttctcttgc aatttttcgc attgctacac
     1321 tacaatgagt cgcaattttg aagtaattat gaatcccaat aaccatactg ttatagactc
     1381 caatatagat aaaggtatct ttgctattag gacttttttg aattcgtttg atttgttttt
     1441 ttagtttcac tttaatcatg ctttgtgctt ttgtacctat atgcgtatta acaacctttt
     1501 tgttaccttt ggtcactaat ttcatcgtaa agcctaaaaa ttcactttct tcttttgaaa
     1561 gatttgtgat ttttgacttt tcttcagata ttggtaactt tagacgctcc tgtaaccaca
     1621 ttttagttgc atagaaaact tttttcgctt cattataatt tctacagaaa attttaaaat
     1681 catctgcgta tctcactagg tatatttctt ttagagttgt actctttctg agcttgtgat
     1741 agatattacc tttatcccaa gctccatttt tcctaaaatg atgttttagc tctttggcat
     1801 ctttttcctc ccactgattt gctatccacc agtcaaattc gttgagattg atgttcgcaa
     1861 gtaatgggct aagtattccg ccttgtggtg ttccttttgt tgggtatatc aacttcccat
     1921 ttggaagtac tacgggtgct cttaacattt tcctaatgat taagagaagc tgtttgtctc
     1981 gtatacccat agaccagatt tgtctcatga gtttagtatg attgacttcg tcgaagaaac
     2041 ctttgatatc tacatctacc acatattcca tgtgactttg attcattcgg gccatacaat
     2101 ctgctattgc gtgttctgct gatctatttg ggcggaaacc atagctgtga ttattgaatt
     2161 ttgcttcaca tattggttct aatacttgta agatgcattg ctgcgcgatt ctatcccata
     2221 ttgatggaat acccaacggt cttaatttac cattatattt aggaatttct acacgcctta
     2281 ctttccgagg gttataatgt gcgaatcgct tcctaacaat ttctacgaat tcattacggt
     2341 tgagattttc aatatcttta atcgttactc catcttctcc tcttgtcaca ctaccgtgat
     2401 ttctcttgat attacgatat gctaggacaa tgttgttatc agaaatgata atctgcatta
     2461 gattcttgaa attatctccc ttcaaacttt tggagtatat cttatcgaag atttccgtca
     2521 tgccataata ttctgcatgc cgcaacttcc tatcttttgg acttgtttct tttgtcatgg
     2581 tttatcacgc tccaattggc tcgtgacctt tttagtcata cgagaatccc atggttgaac
     2641 gggtttcatg acttttacag acgaatcgag cctgttcctc cagttccatt acagaacctt
     2701 catcagtcgt gactctactt tcaccatcac taaagtaagt tatttgtcat tcacatattt
     2761 cttactacca cttcatcgag tatgcactct ccatgttgat ggcttcccct gttcccttat
     2821 atctatccac atgccttaac ttagatgcta gcttttgccc cctaacttct cctacgcctg
     2881 taacgcagca tggtctttca taacaacgac ttttactcaa ccacaagtta gcacaatgag
     2941 tgattttttg ctcactgtct ctcccatttc taggagctat gcctttcgga cagtacattc
     3001 gctagttcgt cagtacgttt gtacatactc accatattaa gtttttttgg acacccgact
     3061 tatcggatac accctttacc tctaaagaac tttcgtggta ttcactattt cttttacctt
     3121 acggtatcct tcagccgact tcaccgagct tcacacaacg ttggtctgca taaactactt
     3181 gttgcatgtc ggagtatcag tggaagccct ttgtggcgtt acccactcat ttgacttctc
     3241 gttataagag ttattacaat agtttttaga ctacttgcaa cccacagctt ttcactgcag
     3301 aacaggtcgc accattatgg cactcatcaa tcatcgccaa gaagcgtttg aagtcttgtt
     3361 cttccatttg tttttcttta agtaaacgtc gttgtcttct tccttgaatc aatgcctgat
     3421 tccaaatcaa cgtcatagcc atgtacattt gacattgttg tacccctcga tctaagcctg
     3481 taatagagtc aatgtcaaaa aagaccaact gttgttggtc aaggtgtcga atcgtactgt
     3541 gaccatcaaa aatcatgcca tacgtctcac ataaggattc tacgatggat tccatttctt
     3601 cttttcgatt ctttttctct tgggaagcac ctggtaattc cagttgtcct aaaaagtcta
     3661 aaaattcttt gaacgtcgga taagcttctg gatctttccc tgtgacttgc gtctcagtat
     3721 ttgaacgcga atttgaggaa gtaaagccgc gtggtactgt tccatactct tcataaaaac
     3781 gatttaagta ggtcttaaat tctgctttca tcgttccttt tagctgagga ctcactaact
     3841 gtacctgata gactactttg tctaagtgtg tttgatagga cgctgcttca ttgatcttca
     3901 aatctttgac tgatccatcg tcatataaac tggatgcttc atcatacgtc cgtgtggcaa
     3961 atatttctaa aggattgatc atcccgtcag acccgtctaa gcgcaccata atgccgttat
     4021 attcctttaa gaaggggata aagtctttcc ccttttcaaa gccccaaata aagtctcctg
     4081 cagctaaatg ggcttccgcg atcattttaa gaacggttga tttaccagct cccatcattc
     4141 ctagaagcaa ggtattagta gacaaccgca cactattttt atatgtcgca ttccagacaa
     4201 atgggccacc tgtcatggtt tccccatatt gagtccctcc tggatctttt agcgtttggt
     4261 ggtggaacgg gaatcctccg ccaatattta acgatggtag tgattgccct ttgatatttc
     4321 caaaccattc agcttgttca tcgaacatgg taaacatcga ttgccaatct cttttcgttt
     4381 tgtgcagata ggtcactgca cgataatcca tcccttttaa attttcaatc agttcctgcg
     4441 ctcgttcatc tacttcttga atcgtgtttc cgtaaacatg tactctcact ttgacttgct
     4501 tagaaatttc cccattagaa gtcaatttac gagcatattc cgctagttcc aggtattctt
     4561 ctgcgctttt atttcggtca gtcgcttttc gttcattatc tgatcgatca tttaattcat
     4621 ctaaggcacg attgatttga tcaatcactt tttgcttttc atctgtgcta acatcgagtg
     4681 tggccactgt atgaggattg ttcatgagat tggccaacca aaaaagcgta ggattttttg
     4741 tgtagtgata aactgtcaca catcgtgaat aaccattacc atacgtcaca tattcttcat
     4801 caaaatttaa cccaccttgt ggcatcactt gttttaaaaa atctaaatca taccctttgg
     4861 atttcaaaat ttctatttcc cgatttccta gttgctttaa tggatcaagt actttttgat
     4921 cattttgtgg tgaaaaagct gttttaattg cttctaacat gtacagtcac ctcttcttgt
     4981 ttgattcttt acttggttaa aagattgccc ttttcccaac ttacccaggc ttaaagtgtg
     5041 aggaaatttt ctagtggtcc cttccttcgg aacatctttt tctaaccata tcaatggata
     5101 tttcattctc tcttctccat tcctactcat cattcatatt gttcattcga tacagaatct
     5161 tgatcaattc ttttttgtag agtggtttta accctaaaat gccactagat cgcattaact
     5221 gacggtttct ggtttgtaaa tccttgattg tctttccttc aatgacgaaa aagaagctta
     5281 agtcaggacg ttcttgttct gctcgtttca attcctggat ttcttcgact aatttctttt
     5341 catatttttt taatcgttgt agatttcttc ctaaggattg ttccatacga ttcttatcta
     5401 ataactgccg cttttgcacg atttgttccg ttagatccgt ctctgtagtc aaactaacta
     5461 acttaattgt tggcacatac gtacgcatga cggtggtcaa ctgattcatt cgttccagtt
     5521 tctcttgttc atttaatccc tccacgtcag tggaaacgac acgataataa cgttgaaaat
     5581 gatcttcttt agtaactaag actccctctt ctgtacagga tttataagga aataaatcca
     5641 agatagatcg ttcttctggt tttccaaact gcaaaataga tttcttatca actgaatttt
     5701 gtcctttgac tcgcatttat tcatcctcct tatacgtacc atgataattt cggttaggat
     5761 ctaacgcatg ataatggttc ttgtccgcaa aaaaaataga ctcgataatc gcaaaaattt
     5821 gtcgttgacc tgggttatta tagggtcgcc aaacaatcac taacgctatc gcaatccata
     5881 agccataatt caagagtgca aaaaagagtg gtaagtggac aagattattg accattttcg
     5941 cccaaaaaaa gcagccacca agcgttaata gatctaaaaa gaaaaaagcg aaaaacttcc
     6001 attctccact aaatcttgga gggtttttca tgtttatata tttatccata attgatttct
     6061 ccttttcata taaagaaaag gcctaacttg taggcctttt atggtttgat atcctgtttg
     6121 atatccctca atagttgttc tttcaatgca ttgttcctgt ttttggtatc tcttgtcaca
     6181 tttatttttg tcggtgcagt aaatgccaca tttgctggct tgtaacctgt atgaacgttt
     6241 tgaataggtt ttggttcagc ttctcttgtc acaatatttt gaacggacgc attctctgtc
     6301 tcaccacttt cagataaatt gtaagaaaca gttgtcaaat tcccattttc aggcaaacga
     6361 cttgatttca tttgaacagt acctgcaggt gattgatgaa caaagctttg atcattctct
     6421 ttgaattgat tcaaacgact atctttcatt ggtaaatttt gtgtcttagg caaaccaatt
     6481 ttcgttgatt tttcagttcc aacttcgatg ccattatcat ttggcagatc gatggaatca
     6541 tcatccgttt gtacttcttg atttcgtttt ggtaaatctg aaatgcgctt ttcattttca
     6601 gaattaatgt tagatttata acgatattgt tctggatctg tttttggtaa ttgaccattt
     6661 tgtccttctt ctgaattttc gttagaatgc aactcatctt ggtgatcttt ggctgaagga
     6721 atcgctgcac cttcttgatt ttctttttcg gttgttgtat tagcttttac tgctgcattg
     6781 acttgtcctt caccatacgc atcttttagt ccttctttga tttctgaagc tttttctttt
     6841 tgtttttcta gtgtttcgga tggattcttc acagcttcag ccaattcttt agctctcgtg
     6901 ccgtctaatt tctcttctaa ggtgtctttg gctttttctg taagatcttc agctgttcct
     6961 tttaacccac gttcattgac gtaaccaaga ccttctgcgg ctttagaaac accatttttc
     7021 gctttcccga acatttcgga aactgttttc ttactattag gtacagttgt agctttatcg
     7081 tctgattgtt cttccccgtt atcaggggta acatcttttc gattaccaaa tccattttta
     7141 cgaatatcat ttaaattgct taaaccactc ttaccagcac ttgcaaggtt tttagccccg
     7201 ctacccacat ttttcgcaac atttcctaag gcaaacatgc taagaagaga attgctgccg
     7261 tttttcagac tcgtatcaac gccgaaaatt ttcgtaactg attgagagcc tttgaatagc
     7321 gcaatggtta atacaattaa agcgacaaca tacaagaatc cattgatctt tcctgccgca
     7381 tgcaaatcac cgatacctga aagcattaat gtatagattc gtaattctaa aatcgtaaat
     7441 gccaataaca agaaggattg agaaatatct tctatagcac ttttcatccg ttgtcctgtc
     7501 tcaaagtccg ttgacgcggc aatcactaaa tagaattttt taaataccag ctcaataatg
     7561 caagtaacaa tcgtaaacaa gataaataaa taggctaccg acaaagaaga ttcaccagct
     7621 aaaataatac ctggactact aatgtatcgg cgatagcctg aagtatacac atattttgct
     7681 aaaccttcat cactaatttg aacagggatt tctttcccat tatcgtctaa ctctagtcga
     7741 tactgtaatg cctttaaccg atctttttgt actgtactaa tatcacttga ttcagacatt
     7801 ttcttagctt gatcaggtgt aataatccca ttgatattcg ctgttttaaa atttttcgcc
     7861 gtgagagcat ttcttttgtc tgttggtatg tgttctactt tgttcggatc attttctagt
     7921 aacgtactca catcaatcac attattttga ataatttgaa aagcaggtga accagtttta
     7981 tctgctttat ttgctgcagt cacgtctccg taaaagtttt tactaatatt tagcccttca
     8041 tcgattaaag aactaccacc aaaaatcaaa atcatggcca taaatagatt aaccaagata
     8101 tttttgattt ttggtggatg tttactcata aaaaatttta agcctaaata aattaatgtg
     8161 acgacacaaa aaagagccaa taccttacta atcaaacttt gaaacagttc attaacacca
     8221 gcggcgttca ataagtcttt taaatcaaag ctttgataaa ttgttttttc tagtaaagta
     8281 ttgagattat ataacccttt aatcaaaccc cataaaaaag tattacaaat ccaactgaat
     8341 acatcttgat actgcagata attaccataa tcttgtaaga ctttcgttac ggttttggga
     8401 tctgcatccc catccccaaa taagcctgac caccagtcgg caatgccgga aaatattcct
     8461 ccaatgatca ttctttcacc gccttttcag cttctagcaa aagtcgtttc cttataacct
     8521 ctgtaatttc ttcattttga cgcaattttt caaggtattc ttgcaaacga tctatttgct
     8581 tctcttcata aaattgaata agtaactttt gtgccgttga gttgtctaaa gattgaacga
     8641 ttttaataaa aatgttttct ttagcttcct cttctatttg atcgtgcata ttgtgataaa
     8701 gtcgacgtgc atagatatca ggtagaaaat aatcgtaatc aatttgtaaa gccttaagat
     8761 ccaagtccgc atgtgtacaa tgtatgtcta tatcattgat atcccacgat ggattgaaat
     8821 cttcaatata ttgataagca taaggaagat tcgtttcccc atgattaaaa ataggatacg
     8881 gccgtatttt atgacctttc aaatcttgtc gttttaaagg agcgagtaca atcgtttcac
     8941 cttctaaaaa agtagaaatt ctttcaggag ttaagatgcg atctgaatca acattgcgat
     9001 tgatatgagt attcatacct aatgaattga tggtactgtt ctcttgttca accgtcttat
     9061 tccccacttt tttggaaaat tcttctatgg tattatcgtc tgtcgacata atataaatat
     9121 gtgtctgaca attttctttg ataatcgctg cgttttgttc cccatacttc gcataaagtt
     9181 gctgatagga ttggataaac aaatcccaca acatgtttct accagctgtg actgtcatga
     9241 ttccttccat attatccatt ggtggcatat tcccaaattc atctaaaata aaatgaatcc
     9301 tacggtgaca tttatctcct ttagtcttaa cacattgcgt agataattct ttatacaact
     9361 gactaagaaa aatactaaca atgatatggt tagatggatc ataatcaggt gtgagcataa
     9421 aaatagccat tggtttatcg gtatagtgca tccgaataga ctctactaat aagttacttt
     9481 tatcctcatc taatgttaga acggcttctt tttctaattc tggcaaatgg gtttctcctc
     9541 gtttttttcg ataaacgata tttccctctt cgtctttcaa tactctttga gaaaactcat
     9601 accaagcaac atattctttt cctcgatgat cgtattttaa gcaaacatga tctccctgtt
     9661 ctaaatgaca atcaaagtta atttcacaaa aacctctata gcctactttg acacgttcct
     9721 catgaaattt ttttccttga tgataaaact ctacaacaat tcgctggttt aacagactct
     9781 cgtgtagttt gagatccaaa tacttcggaa aaccaatgga cttataatca atggttgatt
     9841 gagaagtcat tttcgcaatt ttcgtcagtg taaatggatt tagtcctgtg atcgctgtcg
     9901 atagaatgtt gcctttcgct ttatcagaac caatttttgt tgccccatat tgtttttttg
     9961 ctatgtttcc aggtggtaac tgattcataa attcatctaa agcattgagc tgcttgaatt
    10021 cattatcagg atcttggtaa ttaaaaccac caatctcaga aagaagttcg acgagattat
    10081 tcggtgttaa tttggagtaa tcccctgtct gatccgcttg ttccaataac gcaaaaataa
    10141 gcgcattgac agctttttgc
//
LOCUS       DACEND010000094        10133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEND010000094 DACEND010000000
VERSION     DACEND010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.097"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1228
                     /locus_tag="IW746_001984"
     CDS             <1..1228
                     /locus_tag="IW746_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1078880.1"
                     /translation="LKGKVVLGFDPAYRTGCKLAVVDATGKVLAIEVIYPHKPAAQAK
                     REAAGPAFIQLINKYQVDMVAIGNGTASRESELFVAEQLKSADHKTYYAIVNEAGASV
                     YSASEIARKEFPHLQVEERSAVSIARRLQDPLAELVKIDPKAVGVGQYQHDVSQKRLA
                     EQLDFVVETAVNQVGVDVNTASPQLLQHISGLNKTTAQNIVIYREENGEFTARTQLKK
                     VPRLGPKAYEQAIGFLRVPGGKNILDNTGIHPESYSIAKEILTSVQLSEKELGTEEAV
                     EKLTRLSVEKLAESLSVGEETLADILAGLTQPGRDMRDEMPAPLLRTDVLSMEDLKPG
                     MELTGTVRNVIDFGAFVDIGVKQDGLVHISKLSKKFVKHPTDVVSVGDIVTVWIEQVD
                     TKKGRISLTMLSPYEE"
     gene            1248..1730
                     /locus_tag="IW746_001985"
     CDS             1248..1730
                     /locus_tag="IW746_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1078881.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1746..2507
                     /locus_tag="IW746_001986"
     CDS             1746..2507
                     /locus_tag="IW746_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1078882.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2638..3972
                     /locus_tag="IW746_001987"
     CDS             2638..3972
                     /locus_tag="IW746_001987"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1078883.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            4279..4764
                     /locus_tag="IW746_001988"
     CDS             4279..4764
                     /locus_tag="IW746_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1078884.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            4757..5440
                     /locus_tag="IW746_001989"
     CDS             4757..5440
                     /locus_tag="IW746_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1078885.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            5566..8211
                     /gene="polA"
                     /locus_tag="IW746_001990"
     CDS             5566..8211
                     /gene="polA"
                     /locus_tag="IW746_001990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1078886.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            8256..9092
                     /gene="mutM"
                     /locus_tag="IW746_001991"
     CDS             8256..9092
                     /gene="mutM"
                     /locus_tag="IW746_001991"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1078887.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            9056..9685
                     /locus_tag="IW746_001992"
     CDS             9056..9685
                     /locus_tag="IW746_001992"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1078888.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            9737..10069
                     /locus_tag="IW746_001993"
     CDS             9737..10069
                     /locus_tag="IW746_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1078889.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         3424 a         1805 c         2223 g         2681 t
ORIGIN      
        1 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
       61 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
      121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
      181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
      241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
      301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
      361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
      421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
      481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
      541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
      601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
      661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
      721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
      781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
      841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
      901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
      961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     1021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     1081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     1141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     1201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     1261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     1321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     1381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     1441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     1501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     1561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     1621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     1681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     1741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     1801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     1861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     1921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     1981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     2041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     2101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     2161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     2221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     2281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     2341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     2401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     2461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     2521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     2581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     2641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     2701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     2761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     2821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     2881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     2941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     3001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     3061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     3121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     3181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     3241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     3301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     3361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     3421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     3481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     3541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     3601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     3661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     3721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     3781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     3841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     3901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     3961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     4021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     4081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     4141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     4201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     4261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     4321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     4381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     4441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     4501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     4561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     4621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     4681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     4741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     4801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     4861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     4921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     4981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     5041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     5101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     5161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     5221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     5281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     5341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     5401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     5461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     5521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     5581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     5641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     5701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     5761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     5821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     5881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     5941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     6001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     6061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     6121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     6181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     6241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     6301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     6361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     6421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     6481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     6541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     6601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     6661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     6721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     6781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     6841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     6901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     6961 ttgagccaag aactggcaga taaaaaccaa gaagatcttt tccggaaaat ggaactgcca
     7021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
     7081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
     7141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
     7201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
     7261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
     7321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
     7381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
     7441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
     7501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
     7561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
     7621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
     7681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
     7741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
     7801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
     7861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
     7921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
     7981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
     8041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
     8101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
     8161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
     8221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
     8281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
     8341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
     8401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
     8461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
     8521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
     8581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
     8641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
     8701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
     8761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
     8821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
     8881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
     8941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
     9001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
     9061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
     9121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
     9181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
     9241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
     9301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
     9361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
     9421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
     9481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
     9541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
     9601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
     9661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttactatcct gagtatatag
     9721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
     9781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
     9841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
     9901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
     9961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac atttccgatg agcaaaaaat
    10021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    10081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttt
//
LOCUS       DACEND010000095        10074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEND010000095 DACEND010000000
VERSION     DACEND010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.098"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            299..2134
                     /gene="tdc"
                     /locus_tag="IW746_001994"
     CDS             299..2134
                     /gene="tdc"
                     /locus_tag="IW746_001994"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1078890.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2439..3917
                     /locus_tag="IW746_001995"
     CDS             2439..3917
                     /locus_tag="IW746_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078891.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3883..5214
                     /locus_tag="IW746_001996"
     CDS             3883..5214
                     /locus_tag="IW746_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078892.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5328..7880
                     /locus_tag="IW746_001997"
     CDS             5328..7880
                     /locus_tag="IW746_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1078893.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7887..9326)
                     /locus_tag="IW746_001998"
     CDS             complement(7887..9326)
                     /locus_tag="IW746_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1078894.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9301..9483
                     /locus_tag="IW746_001999"
     CDS             9301..9483
                     /locus_tag="IW746_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078895.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9672..10034
                     /locus_tag="IW746_002000"
     CDS             9672..10034
                     /locus_tag="IW746_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078896.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3208 a         1651 c         2135 g         3080 t
ORIGIN      
        1 tttttaacaa tataaaaaaa taaaaatatg tttagtgaat gaaggacagt cattttattt
       61 gtttgttcgc tttttttagg ttatctattt tagaagactg atttattgaa aaattgttcg
      121 cttttttgcg ttgaattatc agaaaaatcc gactttattt atttttgttt gtaaaaatct
      181 acgtggattt gatgttataa aaaaaaaatt ctcatttttg gcgctatagt gaagaggtgt
      241 tacagataaa cacatgtatc tgcaagacat tgaccaacaa aatatggagg gaagaaaaat
      301 gaaagatatg gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg gaccagttta
      361 taaaatgttg ttgaacaaga tggtagatga acatcttgga tggagagaaa attatattcc
      421 ttcagatatg cctgcaatta gcgaaggaga taagctcact ccagattatc tagccacccg
      481 cgaccatatg atagaggtct tggatgaagt cagtcagcga ttacgcgcag gatcgatccc
      541 atggcattca gcaggccgtt actggggaca aatgaatgct gaaacattga tgcctgcttt
      601 attagcttac aactatgcga tgctgtggaa cccaaataat gttgcattgg aatcttctat
      661 ggcaacttcg caaatggaag cagaagtagg acaggatttt gcttcattat tcaatatgac
      721 agacggatgg ggacacattg cagcagatgg ttctattgct aatcttgaag gattatggta
      781 cgcccgttgc atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg aaaaagtgaa
      841 aaaaatgtct gagtgggaac ttttgaattt atctgttgaa gaaattttgg aaatgaccga
      901 aagctttacg gacgaagaaa tggatgaggt caaagcagct tcttcccgta gcggaaaaaa
      961 tatccaaaga ttagggaaat ggctggtccc tcaaacaaaa cactattctt ggatgaaggc
     1021 gttagatatt tgcggagtcg gcttagatca gatggttgct atcccagttc aagaggatta
     1081 tcgaatggat attaatgctt tggaaaaaac aattcgtgaa ttagctggac aaaaaatccc
     1141 aattctaggt gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag acagtgtaga
     1201 taaaatcgtc caattaagag aaaggttaaa agatgaaggg atctatttct atttacatgt
     1261 tgatgccgca tatggcggct atgcgcgttc actgtttcta aacgaagcag gagaatttgt
     1321 gccgtatgct tccttagctg aattctttga agaacatcat gttttccacc actgcgtaac
     1381 gattgacaaa gaggtatatg aagggttcag agctatttca gaagcggatt ctgtcacgat
     1441 agatcctcat aagatgggct atgtccctta tgctgctgga ggaatcgtaa tcaaacataa
     1501 aaatatgaga aatatcattt cttatttcgc gccatatgtg tttgaaaaat cagtaaaggc
     1561 tccagatatg ttaggagctt atattttgga aggatcaaaa gctggagcaa ccgccgccgc
     1621 agtctggaca gcacatcgtg tattgccatt gaatgtgacc ggatatggtc aattgattgg
     1681 ggcttcaatc gaggcagctc aaagattcag agaatttctc gatcatctga cttttactgt
     1741 taaaggaaaa acaatcgaag tctatccatt gaatcatcct gattttaata tggtcaattg
     1801 ggtattcaaa gaacaaggct gtacagattt gaacgctatc aatgaattaa atgaaaaaat
     1861 gttcgatcgg tcttcttata tggatggcga tgtttatggt gaacggttta ttacttctca
     1921 tactacattt acacaagaag attacggtga ttctccgatt cgttttgttg aaagaatggg
     1981 tctaacgaaa gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg cggctatcat
     2041 gactccttat ttgaatgatg atcgaatttt caatttttat acaaaaaaaa tcgccaaagc
     2101 aatggaaaag aaactaaacg aaatcatcca atagaactga tttttgtgtg aaagaataac
     2161 aagagacata tggagaacag acaattattt gcttcatcaa aacaattgta tttccccctg
     2221 tttttcatat attcctcgcc aagatattct tttttataaa acgttctata agttggatcg
     2281 ttcgtttcat gtatcaaacc cagttcacta agatcgttca atggatgttt cggtgatact
     2341 gggttttgta ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa aataaaagaa
     2401 aacaaaaaca tatgtcgcta ttgaaagaag gaataaagat gaaagatact ggttcatcaa
     2461 agcaaatcac gaccatgacc tttatcggaa tgacctgcgc ccttgtagca agtgtacgaa
     2521 atattccaga tgtagcagca acgagctgga cgatgatttt ttacatgctg gttgcagtat
     2581 tattgtacgc ttttccaatc tcattgatct ctggtgaatt tgctggaatg tttccccaaa
     2641 aaggtggtcc agaactttgg gtatcgaatg ctctaggaaa aaaatggggg ttcgttgtct
     2701 catggctgtt atgggtccaa atgtttcctg ggatggtgat ggtcgcttca gcactcgctc
     2761 cgttgtttgg taatattatc gataatgtgc cgcttggttt gaacagcaaa tttacattag
     2821 ttgtcatttt agtcgtttat tggatcatca cttttttgaa tttgaaattt gatatggcaa
     2881 aaatcggcgg aaaagtcggt gtttggttag gattgtacgt gcctttgacg atgatgctct
     2941 tattagggtt tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca ttaggcagtt
     3001 tttcatggga caaattaata ccagatacta cgactgcatc ctcatttgtt tattttgcgc
     3061 cgatcatgtt tatttttact ggtatcgaaa tgtcctctgt gtatatcact cgattggaaa
     3121 atccagttaa aacgtatatt cgcggagtat ttgccgcgtt aatttttata tttttcgtca
     3181 atattttgaa tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg gaattgaata
     3241 atatcgcgca atctatttcg atttattgcc aaattcttgg tttgccacat cttattgtga
     3301 atctgtttag tttacttgtc ttcattggtg tagcagtcca attatctgct tgggcctcag
     3361 gaccagcaaa gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca aaattcaatt
     3421 tttggaagac gaatcaattt gacgtatcca aatccgttat tttgactcag tctgtcatta
     3481 tctcaatctt tgcacttttt tatttgctga ttcctggcgt caatcaagca tttttgatgc
     3541 tcgtaaattc aactactgtt atctactgta tcgtctatgt catcatgggg atcgcagttt
     3601 tgagattgcg ttatacacat gcaaagttga atcgaccatt tagaattgga aaaaaagaga
     3661 aaaagagcaa tcttggagtt tggactgtcg ttattgtttt atttgcggca atcggatttt
     3721 ctgtcggact gacgatgaaa gctggaacat ggatcaatct aattgcagtc acagccatca
     3781 gtgtcatttt gtttgtagta ccactctgta ttgaaaaaat aaagaaacct tcttgggaac
     3841 aggaagtcgc catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa accggcaatg
     3901 aaacggactc taactaatcg aaatatccag ttaatcgcat taggtggcgc aattggaaca
     3961 ggattatttt taggttcagc aaaggcaatt gcatcagcag ggccagctgt tattctcgtt
     4021 tatttgatat gcggtttggc tatttatggt ttgatgcggg ccatgggtga tttattttta
     4081 gcaaatagta catttaactc tatcagtgat ttcgttgact attatttagg caaaaagtgg
     4141 gcattttttg tcaattggac ttattggctt tgctggcttg gagctggact tgcggaatta
     4201 acagcaatag gattgtatat ccatttctgg tttgtacatg taccagcttt gataagcgga
     4261 ggtagcgcac taatgatctt aatggcagtc aaccttgttg cagtcaagtg gtttggtgaa
     4321 ttggaatctt ttttctcaat catcaagatc ttggggattt tcctttttat cttattgggt
     4381 ataggattga gtgtctcaga ttttcatacc cagtctatcg gaatcaaaga aaattttgga
     4441 cagatcgatg aattactacc gtttggcttt gctggaatgg tagtctcatt tcctatggtt
     4501 ttattcagtt ttgctggaat tgaaatgatc ggcttaaccg ttggcgagac aagtgatccg
     4561 caaaagaatt tgaaaaaagc aatcaactgt ctgccttggc gtattctctt tttctatata
     4621 ggaacgattt tgtcgatttt atttgttgtt ccatggcatt gtttagattt aaaagaaagt
     4681 cctttagtga ctatgctgca agtgatccat tgccaaggcg gagcagcaat tgtcaatcta
     4741 atgattttgg ttgcttcttt gtcctcagca aatagtgcag tctacagtac tagcaggatt
     4801 ttatatcaaa cagcaaaaga aaaaagtttg ccccattggt tttgtgcgct ttctaagaaa
     4861 ggcgtacctt tacacggtat tttaattaca ggaatgttat tttttgccgg tctttgtcta
     4921 catttttttg tagctgatag tcgattgttt tttcagcttt tggcaggcat gacaacgagt
     4981 tgttttctat ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat taaagaaaag
     5041 aaacggaaca ctacacaaag atataaagat cttgctttgt tggtgttttt tacttcaatt
     5101 gctttcagct tgttattcga aagcacgaca cgctttattc cttttttatt ggttatctgg
     5161 tttgtattct taaattttgt atataaaaga ataagcaata gaaatatcag ttaggcccag
     5221 atacattttg caaaacgaat tatttcagac ccccctgaaa taatcgatac gcctgaaaaa
     5281 acttccctgc gtataataac agtatcaatt aaacaggagg ggataggatg aactggtaca
     5341 gattgacggt agaagatgta ttgaaaaact atcaaacatc aacagaaggc ttgtcgcaag
     5401 aagaacgaca gaaacgcctg actgatcaag gatataatga aataaaagca aggcagcaga
     5461 caaaacgatg gaaaaaaata gcaaagcatt ttactgatct gctgatgatc gtgttgatca
     5521 ttgcctcttt tttgaaattt gcttctagtg aatatattga aggaagtatt attttattcg
     5581 tagtgatcgt caatggactc gttagttact ggcaagaaag aaaagcggaa gaatcattga
     5641 atggtttgaa gcagctgatg ggacaagaag cgatagtact atcaaacggg atgcaagaaa
     5701 aaatccctgc gaaagaactg gtaaaaggag atgtcattgt cttacgtgcc ggagatgtcg
     5761 ttccagcaga tatccgatta atcgaagcac atgatttatt agtggaagaa tcgattttga
     5821 cgggagaatc agaagcatct gaaaaaagtc atgccgtttt gacagaagaa gaatcgatcg
     5881 gcgaccagaa aaatatgggt ttttcaggaa cgcttgtaca atctggttct gcagtcggtg
     5941 tcgttgtaga aacaggaaac acgacagaaa tcggtaaaat caatcaagca ttgcaatcgg
     6001 tcgaacaaca aacgacgcct ctgattaaaa aaatcaaccg attaaacaaa caaatttttc
     6061 aaggcattct ttgtttgagc ctattcttag ttatttttac gacatttcga tatggtatgg
     6121 attggcatat ccttctttcg gctacgattg ctttggtggt gtcaatggta cccgaaggat
     6181 tgccagctgt tttgaccatg attctatccg ttggagtaca tgaaatggca aaagaaaagg
     6241 cgatcatcaa aggacttcct tcggtagaaa cattaggttc tatgacagtg atctgttcag
     6301 ataagacagg aacattgaca aaaaatgaaa tgacagttgt ggatgtagct gcaaaagaag
     6361 aagcctgcgt gttgtccatt atgaaaaact gtcaggaatt aaaaacaaaa gaagaacaga
     6421 aaacagaaaa tttaagtggc aatccgacag aagttgcgct gattcgacac acagaaacag
     6481 ccaatctacc tttgaaaaaa atagaagcaa agattccgtt cagttctgaa tacaaatata
     6541 tggcaacgat gcatgcggag gaaaaaggag cggtgattta cgtcaaaggt gcaccagaag
     6601 tattatttgc taaatcaact ctttcttctg cagaacaaga agcatggagt cagacagctg
     6661 cagaatttgc gagaaaaggt cagcgtgttt taggatttgc ttataaaaaa gtagattcga
     6721 aacaggaact cacgcatgag actttaaccc aattaacttt tgcagggatc gccggattga
     6781 tcgatccgcc aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa gcagggatct
     6841 cagtgaaaat gattacaggg gatcacaaag acacagcaaa agcgattgcg gaacaaattg
     6901 gcttaaaaca tactgccaaa gttttagaag ggatcgattt ggatcttatg tctgatgaag
     6961 agttgattca gcaagtgcca atagtcgatg tctttgctcg aacgacacca gaacataaac
     7021 tgcgaattgt taaagctttg cagaaaaatg gagaaatcgt cggaatgacc ggagacggcg
     7081 taaatgatgc acctgctttg aaaagatccg atgtaggaat tgctatggga atcaaaggta
     7141 gtgaagtaag caaacaggca gctgatatgg ttttaggaga tgataatttc catacgattg
     7201 caaaagccgt aaaagagggc cggcggattt tggataattt gcaaaaaacc atcaatttct
     7261 tcttgccaac tgcattagct caaggattga tcttgatttg ggcacttatg ctgaatcgcc
     7321 cgctaccttt gtccccagta cagattttat gggtcaatat ggtaacgaca atcactttat
     7381 cttatgcact agggttcgag tcgccagatc cagaaatcat gaagcgccca ccaagagatc
     7441 caaagcaagg aatcctatct ggttatcatt tgtttcggat catctatgtc tccctactga
     7501 tcatggttcc tgcctattta ttggctatgc agtttgaagg gcaaagctta cagcaaacca
     7561 ttcttctgca aaatatcgtc ttagctcaag ctgtttacat gatcaattgc agagagttgt
     7621 ccaagccgtc attgaaccaa ggtcttttgc aaaacaaagc gctttttctt tcattaggta
     7681 tccttgcttt attgcaaggg atggttattt ttctaccagt cggccagcag ctgatcggta
     7741 cagtaagttt aacgatgaca caacagttat tgattggagc aaatgtgatt cttctattct
     7801 tagttgtaga aatagaaaaa gggatcatga acaaactgtt tcgcaaaaaa gaaaaaacag
     7861 ctagagaata cagtcgctag ccgttactat aaaaaagttt tgaaagtgag ttcagtcaaa
     7921 cgattgagct catttttcgt tggaatagaa gaaatgtata caatagggat atctagagaa
     7981 tctagaggga aattggaaat caagatatct gttttttcaa aagaaatagt ggaaagatcg
     8041 gttagttcga taggtaagaa gcagacacgt tttcctaagt aatcttttaa ttcctgctgc
     8101 aagaacgcct tccatgaagg ttctccttgg aaaataaaat agacattgaa caaattcggt
     8161 ttgatagaaa aaatcgtttg ttggatcagt agagacaaga gttggagaaa gtaatttggt
     8221 tcattgattt ctatttcttt ttttagatcg ttcagtaaat tctctttctg ttgatacaag
     8281 tgtgcatatt gctgatcata tgtttctaaa aaacgatggt attgacggtc caatgcttca
     8341 atcaatatcg gcgattcatg atattttaat atgaggagag ccaagttatc agtcagagat
     8401 gtttttttta gttgatcaag agacagctct gaagctagtt cctctacaaa cgtttcgagg
     8461 acctctaaaa atggagaatg atgcaggcag cgagcggttt cttgtccata gttattgttg
     8521 taattccaac tttccagaaa gcagaaagaa gcaaaagaaa gctcagtgtc tttaagataa
     8581 ataccttccg ttaaatacaa ttttttcaat gcagagtcat acagggagga gagtgtgctg
     8641 ttggtatgga tagaagaatc taatcggcaa ttcgctttta tacggatcgt attgataaag
     8701 aaccaacatg ttccgaaaat ctcttctgtt tcaaccgcta gttcagtttg actgaggttt
     8761 cttaaaaatt gaaaataatg ggattcatgg atcgaataat cctcgaaaaa atagttgtta
     8821 tgagtgaaag gaatcagcaa tcgatgataa aaagtacgaa tcgcacgctc ttttccaaga
     8881 atacaagctg tatatttcgt caaatcgatc tgcaagtcgt aacttgctaa acgctgattg
     8941 attttagtca catgtctttt caatgtccca taagaaagcc catggtcggc tagaaaatca
     9001 gggacagcaa ctttttttgt aaaaagcaga tttttcagca gttcgacact gactgattgt
     9061 ttgaaaaaaa tctcccaaag gtcatttgtt aataaatttt gctggttttg caaactgatt
     9121 cctgcttgat cgctgatgat gtcccagcct tcgggcagct gagagcgaat agattgaagg
     9181 tcagaaaaaa tcgttcgttc ggttgtccct atgatttcag ctaattcatt tacggttatc
     9241 tttgtatgat tcagcaattg ttctactaaa gaaacatgac gtcgaatatc tttttcggta
     9301 atgatttttt tcattagtga atacatcaat cagactccct tctattctaa gaatacggag
     9361 aaaataaacg gtggtcaagt ttcaactgat ttttcaatca ttgtttattt ttctggagca
     9421 tttactgatg ataagaattt catcatgaac ttgaaaaatg ataccactaa aatcaaaaaa
     9481 taggtgggca aacaataata aacattttaa tagtagatgg tagcaagacc aacacaggcg
     9541 gattgaacat ttaagagttg ataaaaaaga aaaatatcga agtatttgat tagcaactac
     9601 ttgtattcat ttttctattc ttacgtggta tctttgaata aagcgaatca ataagaaaga
     9661 gatggaagca gatgctaacg aaaaaagaac taatcgatca catcaatgaa acatacaatt
     9721 tgtctccaga atatattttt aaaaaatttc ctgattattg cgtattcaga catcaaaaaa
     9781 gcaaaaaatg gtttggggtc ctgatgtctg ttccagaaga aaaaatctat gatgatgcaa
     9841 gtgaccatag gatcatcaat attcttgatt tgaaggtcga tcctgaactt ggcggaattt
     9901 tgcgaggaaa agaggggtat tttccagcct atcacatgaa taaagaacat tggatctcta
     9961 ttgatctacg taaaatagaa aagctagatg aactagcaga tttgattgat ggaagtttta
    10021 aatcaacgca atgaatgtct aggacaatag agaagaggtt gtgacagaaa tgtt
//
LOCUS       DACEND010000096        10020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEND010000096 DACEND010000000
VERSION     DACEND010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.099"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IW746_002001"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IW746_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1078897.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IW746_002002"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IW746_002002"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1078898.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="IW746_002003"
     CDS             complement(2159..2896)
                     /locus_tag="IW746_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1078899.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAENPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="IW746_002004"
     CDS             complement(2987..3514)
                     /locus_tag="IW746_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1078900.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="IW746_002005"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="IW746_002005"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1078901.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="IW746_002006"
     CDS             complement(4391..4876)
                     /locus_tag="IW746_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1078902.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="IW746_002007"
     CDS             complement(5029..5226)
                     /locus_tag="IW746_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078903.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IW746_002008"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IW746_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1078904.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IW746_002009"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IW746_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1078905.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="IW746_002010"
     CDS             9201..9413
                     /locus_tag="IW746_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1078906.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..>10020
                     /locus_tag="IW746_002011"
     CDS             9415..>10020
                     /locus_tag="IW746_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1078907.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEI"
BASE COUNT         2885 a         2189 c         1709 g         3237 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa gggttttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
//
LOCUS       DACEND010000097         9807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEND010000097 DACEND010000000
VERSION     DACEND010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.100"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2150)
                     /locus_tag="IW746_002012"
     CDS             complement(30..2150)
                     /locus_tag="IW746_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1078908.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2380..3558
                     /locus_tag="IW746_002013"
     CDS             2380..3558
                     /locus_tag="IW746_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1078909.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3574..4332
                     /gene="aroD"
                     /locus_tag="IW746_002014"
     CDS             3574..4332
                     /gene="aroD"
                     /locus_tag="IW746_002014"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1078910.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4355..4840
                     /gene="ybaK"
                     /locus_tag="IW746_002015"
     CDS             4355..4840
                     /gene="ybaK"
                     /locus_tag="IW746_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1078911.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4911..6164)
                     /locus_tag="IW746_002016"
     CDS             complement(4911..6164)
                     /locus_tag="IW746_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1078912.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6281..7630
                     /gene="gdhA"
                     /locus_tag="IW746_002017"
     CDS             6281..7630
                     /gene="gdhA"
                     /locus_tag="IW746_002017"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1078913.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7742..9088
                     /locus_tag="IW746_002018"
     CDS             7742..9088
                     /locus_tag="IW746_002018"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1078914.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9396..9782)
                     /locus_tag="IW746_002019"
     CDS             complement(9396..9782)
                     /locus_tag="IW746_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1078915.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2010 g         2889 t
ORIGIN      
        1 ttgtcgctgc tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga
       61 agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta
      121 aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca
      181 ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga
      241 agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc
      301 agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg
      361 ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg
      421 aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt
      481 tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa
      541 gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc
      601 atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga
      661 tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta
      721 tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa
      781 ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct
      841 gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa
      901 taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca
      961 tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa
     1021 taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca
     1081 tggaataccg cactaatgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa
     1141 gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt
     1201 tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca
     1261 aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg
     1321 agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg
     1381 atataaatca cattttttcc tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa
     1441 tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact
     1501 tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat
     1561 ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc
     1621 ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt
     1681 gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata
     1741 ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc
     1801 agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat
     1861 tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc
     1921 atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag taataacaat
     1981 gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga
     2041 tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa
     2101 aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc
     2161 ctttctgcac ttcttaatca cagaagtgtc ctgaaaccat gttacacagc tcacttattc
     2221 tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt
     2281 cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt
     2341 tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa
     2401 agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat
     2461 ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca
     2521 aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc
     2581 ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc
     2641 tttttttcag atgattctac taccgctttt cgtatattca atggagaagg agacgggatc
     2701 ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg
     2761 ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcata
     2821 ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac
     2881 ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat
     2941 ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta
     3001 gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt
     3061 tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc
     3121 agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa
     3181 atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat
     3241 gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta
     3301 gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata
     3361 cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa
     3421 gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa
     3481 gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa
     3541 attaaaaata accattagaa ggagcgatgc agaatggttc gggtaaaggg attgaatata
     3601 ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta
     3661 cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat
     3721 tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg
     3781 cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac
     3841 atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat
     3901 atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc
     3961 aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac
     4021 cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg
     4081 gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca
     4141 aatgcttttg tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct
     4201 cgtctggttg gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct
     4261 acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat
     4321 gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac
     4381 gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg
     4441 ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg
     4501 tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc
     4561 gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga
     4621 aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc
     4681 tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca
     4741 aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg
     4801 tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag
     4861 aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt
     4921 tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg
     4981 ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta
     5041 ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat
     5101 ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa
     5161 tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca
     5221 agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt
     5281 ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg
     5341 aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa
     5401 cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc
     5461 gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa
     5521 gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct
     5581 gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca
     5641 gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg
     5701 aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct
     5761 actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg
     5821 ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa
     5881 tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta
     5941 ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg
     6001 ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt
     6061 ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg
     6121 cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga
     6181 aagtgtagca tatctgaaga aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat
     6241 gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata
     6301 tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca
     6361 agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat
     6421 cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc
     6481 ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc
     6541 agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt
     6601 gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg
     6661 aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt
     6721 ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc
     6781 gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt
     6841 gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat
     6901 acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga
     6961 aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta
     7021 tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg
     7081 gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa
     7141 tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga
     7201 atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga
     7261 aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg
     7321 cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta
     7381 ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca
     7441 gaacgtccag cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat
     7501 ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct
     7561 gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg
     7621 tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga
     7681 aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca
     7741 aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt
     7801 agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc
     7861 aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc
     7921 acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg
     7981 tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt
     8041 caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat
     8101 cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa
     8161 cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga
     8221 gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga
     8281 taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat
     8341 tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc
     8401 tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata
     8461 ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct
     8521 ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt
     8581 ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta
     8641 tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg
     8701 acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat
     8761 tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt
     8821 cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa
     8881 cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt
     8941 tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg
     9001 tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct
     9061 tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca
     9121 gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata
     9181 ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg
     9241 tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag
     9301 gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac
     9361 tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt
     9421 acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt
     9481 gctttcaact gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa
     9541 gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg
     9601 tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc
     9661 attggagctt tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc
     9721 caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc
     9781 atgaaagatc tcctttttta tttttat
//
LOCUS       DACEND010000098         9694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEND010000098 DACEND010000000
VERSION     DACEND010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.101"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            65..1492
                     /locus_tag="IW746_002020"
     CDS             65..1492
                     /locus_tag="IW746_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1078916.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1584..1949)
                     /locus_tag="IW746_002021"
     CDS             complement(1584..1949)
                     /locus_tag="IW746_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1078917.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGCILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1946..2311)
                     /locus_tag="IW746_002022"
     CDS             complement(1946..2311)
                     /locus_tag="IW746_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1078918.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2503..2751)
                     /locus_tag="IW746_002023"
     CDS             complement(2503..2751)
                     /locus_tag="IW746_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078919.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2750..2878
                     /locus_tag="IW746_002024"
     CDS             2750..2878
                     /locus_tag="IW746_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1078920.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2935..4629
                     /locus_tag="IW746_002025"
     CDS             2935..4629
                     /locus_tag="IW746_002025"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1078921.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4633..4752
                     /locus_tag="IW746_002026"
                     /pseudo
     CDS             4633..4752
                     /locus_tag="IW746_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4834..5025
                     /locus_tag="IW746_002027"
     CDS             4834..5025
                     /locus_tag="IW746_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078922.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            5403..6536
                     /locus_tag="IW746_002028"
     CDS             5403..6536
                     /locus_tag="IW746_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1078923.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6567..6887
                     /locus_tag="IW746_002029"
     CDS             6567..6887
                     /locus_tag="IW746_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078924.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6916..7380)
                     /locus_tag="IW746_002030"
     CDS             complement(6916..7380)
                     /locus_tag="IW746_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1078925.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7512..8766)
                     /locus_tag="IW746_002031"
                     /pseudo
     CDS             complement(7512..8766)
                     /locus_tag="IW746_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8795..9112)
                     /locus_tag="IW746_002032"
     CDS             complement(8795..9112)
                     /locus_tag="IW746_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1078926.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9138..9458)
                     /locus_tag="IW746_002033"
     CDS             complement(9138..9458)
                     /locus_tag="IW746_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078927.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3135 a         1905 c         1733 g         2921 t
ORIGIN      
        1 aaaaagaaaa catgtcataa tatttcccat attgtgcaaa aaaatacaag gcggaggctc
       61 tactatgatt gaagaatata ttgagaaaaa tattttaaga cagctgtttt tatgtgggca
      121 attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc atgtttgcaa
      181 aaccacttta ctcaatgata tcaataacat aaaaaaagaa tttgaagaac agatcgctta
      241 tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac ctcgatgcaa
      301 gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc tttatttgga
      361 agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc
      421 ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg aaatcgaccg
      481 ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg
      541 tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt
      601 cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata aagaaatctt
      661 atcgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga caatagattc
      721 aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat cagcatggga
      781 aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt tgacactatt
      841 caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact ttcaacagct
      901 acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta cctttgaatc
      961 tcacttcaat caagaattat tcggaaacca accgttcgaa cgtgcgctga ttcatctgat
     1021 gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc ttttaaatga
     1081 ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt ccagccagtt
     1141 accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg aattgagcgg
     1201 aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt cggatgttca
     1261 ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc aagttgcacc
     1321 aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtcct caaaccgggt
     1381 attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata ttattcctat
     1441 ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat aaggggctgt
     1501 gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt aacaatctct
     1561 tgatacacgt caggctcaca acctcactta ttttggacat ctacgggtcc gatctcttta
     1621 tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg gaaaatatcc
     1681 tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat ttccaaactt
     1741 agaagtatac agcctcccaa catcttttta atagcattgt tctcgctgac tcggtcgctt
     1801 tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag acaaacaccc
     1861 caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc gaaatatgga
     1921 agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt
     1981 cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata ataagtgcaa
     2041 gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca gcaacgacta
     2101 gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct gcattacgag
     2161 tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg aatagaacaa
     2221 gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca atgatccatt
     2281 ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt ttaatttgtt
     2341 cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt tgttctaatg
     2401 ataacggttc gcttaaattt ttctttttca ctctcccatt tttctccctc catccataaa
     2461 tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg tcatcagctt
     2521 ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa tagaaccata
     2581 cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa aatcagacac
     2641 ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc attctaataa
     2701 aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa
     2761 attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat atctaggaaa
     2821 aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat caatctagca
     2881 tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat cacaatgaga
     2941 aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc tgtttatttg
     3001 attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga tgatcacggt
     3061 tcaaatgatg gtgcaccgac cgacactgta gtggatgaat attggaataa aaaccatcca
     3121 ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta ccgtacctat
     3181 gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg gatgactgct
     3241 gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg
     3301 gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa agaccgtatt
     3361 cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga caatatcact
     3421 atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg gtttatgtgg
     3481 gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg ctatatgcat
     3541 caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac
     3601 aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca aggatgcaaa
     3661 atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat gcaagatgaa
     3721 atcactgtga ctggtttgaa aattaccaat acactgactc aagaagaaga atacgttgtg
     3781 gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc agctacttta
     3841 ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc tagtctctgg
     3901 aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt tgctgatcga
     3961 gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt gttgaatgaa
     4021 atcacccgta tcacgactca agaaccagga aatgccttga attcatttat ttcaaccaca
     4081 ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca tcatcaaccg
     4141 cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct ttatccaaga
     4201 cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga aatgctagaa
     4261 gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg tgaaaaagaa
     4321 acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta tgcttccgcg
     4381 ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat
     4441 ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac tgaacaaagt
     4501 gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtattcctat gtcccgtctt
     4561 gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc aaaaaaaatg
     4621 tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccagg
     4681 ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat gcaagctttt
     4741 gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaccc
     4801 aagctaaaac tgcttcttat gtttttgact tggttggact tattaccgca gatgcaagct
     4861 tttgaacacc atttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4921 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4981 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     5041 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     5101 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5161 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5221 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5281 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5341 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5401 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5461 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5521 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5581 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5641 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5701 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5761 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5821 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5881 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5941 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     6001 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     6061 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6121 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6181 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6241 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6301 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6361 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6421 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6481 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6541 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6601 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6661 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6721 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6781 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6841 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6901 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6961 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     7021 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     7081 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7141 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7201 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7261 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7321 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7381 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7441 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7501 tttttgattg atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7561 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7621 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7681 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7741 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7801 atcgttggtt cattaatatt gaaggacacc tggcgcaatc gacaagtttg ttacaaccat
     7861 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7921 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7981 atgcccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     8041 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     8101 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     8161 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8221 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8281 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8341 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8401 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8461 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8521 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8581 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8641 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8701 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8761 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8821 caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8881 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8941 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     9001 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     9061 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     9121 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9181 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9241 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9301 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9361 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9421 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9481 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9541 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9601 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9661 caaaacttgt cacaactttt ttcaaataaa cggt
//
LOCUS       DACEND010000099         9456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEND010000099 DACEND010000000
VERSION     DACEND010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.102"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..814
                     /locus_tag="IW746_002034"
     CDS             62..814
                     /locus_tag="IW746_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1078928.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(908..1513)
                     /locus_tag="IW746_002035"
     CDS             complement(908..1513)
                     /locus_tag="IW746_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1078929.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(1566..2540)
                     /locus_tag="IW746_002036"
     CDS             complement(1566..2540)
                     /locus_tag="IW746_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1078930.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(2918..3697)
                     /locus_tag="IW746_002037"
     CDS             complement(2918..3697)
                     /locus_tag="IW746_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1078931.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(3764..5035)
                     /gene="celB"
                     /locus_tag="IW746_002038"
     CDS             complement(3764..5035)
                     /gene="celB"
                     /locus_tag="IW746_002038"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1078932.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            5188..6585
                     /locus_tag="IW746_002039"
     CDS             5188..6585
                     /locus_tag="IW746_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1078933.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            6666..7106
                     /locus_tag="IW746_002040"
     CDS             6666..7106
                     /locus_tag="IW746_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1078934.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            7254..7580
                     /locus_tag="IW746_002041"
     CDS             7254..7580
                     /locus_tag="IW746_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1078935.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            7581..8330
                     /locus_tag="IW746_002042"
     CDS             7581..8330
                     /locus_tag="IW746_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078936.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(8410..8865)
                     /locus_tag="IW746_002043"
     CDS             complement(8410..8865)
                     /locus_tag="IW746_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1078937.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3024 a         1689 c         1892 g         2851 t
ORIGIN      
        1 gaagctacca aagaaatatt gagagaaaaa agttttataa aaagataatg gaggggtcat
       61 tatgtcagat ggacgtatgt tatttgagag agaacgacag gacatggcac gtatcgtaag
      121 agtgattttc gaaaggaaaa acacaaatgt cgcaggaggg aatttttcct ttaaaaccac
      181 tgatgatcaa gggaaagact acatcatcat gacgccgacc atgatgtcag aagcctatct
      241 aggagagatt tctcctgcac aggttctagt agtagaacca agaacgcgta gaatcgttga
      301 cggagtcggt catttaactc gtgaaatcaa tatgcatgaa gcgatgtatg agactaatcc
      361 agctatcaaa gctgtcctac atgctcatgc gcctaatgca atgttctggg cgactactgg
      421 gttgcctatg cctaatttga cagaagccac agctaaagta aaagagattc ctgtcatgga
      481 ctttgagcct aattgctctg aagaacttgc tgaattgaca agcgactatg taaaaaaaca
      541 gcatctcgaa ttaccgcata tgttgctgct caactctcat ggcgtattga tcaacacagg
      601 tggagaatca ggaatcgaag ctatccataa agcactgcaa atccttgata cagttgaatg
      661 gaatgcagaa attgcctaca agcagacgat ttttcaagca ttagggatta ctgacggttt
      721 ttattcaaaa ggtgagaaaa tcgggggatt ggaagagttg aaacataaaa cggctattta
      781 taataaatct gttattgcag ctggtggaga ctaacctatc tgacacaaaa aactacgact
      841 ttcacaaaat ctgatccaga tcgatatctg cttcaggttg tattatgtaa gtcgtagttc
      901 ttttctttta tttagttaag aggtggtctg tccattccct cgcttgcttg atttcgtcca
      961 tcgtcaactg atgacctgtt acagttagct gaatagtcac atttgcttgg cgtgtttcga
     1021 attgatcggc caattcttta aaggaagcag gggaaacgat tggatcgtta tcaccataag
     1081 aaatccagac ggttttatcc tctaatgtgg aacggctttg atgaacacct agagacatcg
     1141 gatggaaaaa caacccttta tttagctcgg tctcacgctc caataataaa tgagcagcga
     1201 tgtttgcgcc atttgaatag ccgacaagca gccagtcact taacggtatc agttcttttt
     1261 cgctgatttc tttgattttt tcatggagtt tatctgtttc tttttccaaa ctgtccaggt
     1321 cgaattgatt caatccatta cgcttgaaaa aacgattcat cccatcttct tggaccgtgc
     1381 cacgaaaaga aagcaatgta ctgtttggtg caagaaagtg agcgatatcc agtaaagaat
     1441 gttcatcgcc acctgtgcca tgaagtaaga tcaaatgctt gccacttgtt cctttttcaa
     1501 aaatgtaggt cattttgttc cctcttttct gtttctatag atgcaaatga agtagcatga
     1561 agagattatt cacgtttttt atttgcatca cttgtatcga aaggacggac ataagcttca
     1621 atggcttcac gacgttcttc taagaatgga ggaagagata gtttttctcc tgctgattct
     1681 gctggttcat cttcaaggaa tccaggacca tcagttgcta attcaaacag tacgtgatga
     1741 gcggctagga agtactcaga ttggaaatag aaacgatcga cgaaaccaga atttggcaat
     1801 tcaatcttat tgattacttg aatccagtat tccaatgctt ctttgtctgc tacacgcaat
     1861 gctaagtgat ggatatttcc aaatccttcg attgcttttg gcaagtctgt ccgatgttca
     1921 ataatgatgc ttgcaccatt tcccccttca ccgatttcaa ataagtagaa atctcctgaa
     1981 tggtctgttt ctttgaatcc taagattttt tcaagaatca agcggatatg ctctaattga
     2041 ctgacacgga caaagacagg tccaagtcct gtgatcgcaa actctgtcgg tacattgctg
     2101 tttttccaag gaatgccagc tgctacacct tgattgtttt cgtcagagat caattgataa
     2161 tactgattat cgaaatcttc aaattcgata tattttttgc cgaaacgttc attgattgct
     2221 gaatgagtaa cgtcatattt ttcaaaacgt tccaaccaaa agtctaatgc tgcgtcactt
     2281 ggcacacgga aagaagcacg agagatcgta tctgttcctt tttttccttt tggctgattt
     2341 ggaaaatcaa agaaagtcat atctgttcct ggcgcaccag tatcgtctgt aaagtaaaga
     2401 tggtaagttt cgatgtcatc ttggttaact gtttttttga ttaagcgcat tcctaaaata
     2461 tctgtaaaaa agcggtagat ttttgtagcg ctagatgtca ttgctgtgac gtggtgaatt
     2521 cctctgattt gtgtatccat gataaatttc ctcctgttaa cttacaaaaa gtaagtagtt
     2581 gatttagaca tagtatagag ggtgcatctc tattttgcaa ttaatttgtc ttctaaagta
     2641 tcaggaagga atcaagaatg gaaaagagga gggacggatc aataaaataa aaagacttac
     2701 gactagctgt atcgatttca aaaaaactag ctttaaaaag ctaactttag aatcattacg
     2761 ggtggtcata agtcttttta tgctgagtta ttttttcatt tttgagctaa tgcttaggat
     2821 agaatagaat gttgatttca cgatctcttc ttgttacttg aaactgctta ccactgtcac
     2881 gatctcttgg taaacggctt tttcacaacc tctttggtta agttgaacgc acatccagca
     2941 cagaatagtt ccgaacatca aatcggtttc ggctggaaga atattcaatc ggctgtccat
     3001 ctttgagata gacgacttgc tgaacttcca gtacaggatc gttttctgca cagttcaaat
     3061 attgctgatc atattcatca cttttatctg cagaaatagc acgataagcc cctgcaaaat
     3121 ggacattcaa ttcttgatga agatgatcat agatagaggc taacaaatgt tcttttttta
     3181 aacccggcac aagatggact ggcataaagg tatgctctaa aacatatggc tcgccatcta
     3241 agatccttaa ccggtgaata ttgtagacag gttgctcggc atcgatcatc aatcgttctt
     3301 ggatatattg attagggaat tcaacatcaa aatgaatgat ttgactcttc aaagaacgat
     3361 gtctgcgttt catttggaga ctcaaacctt catactcgtt agcgggcgat gtgtcttttt
     3421 gcataaatgg atgattcaag attttcgttc ctgatcctcg ctgcgaatag acaagtcctt
     3481 ccattgctaa gatattgatg gcttttttga ttgtcatccg gctcgcacca aattctttta
     3541 ccaagtcgac ttgattaggc aaaaaactat cgagaggata ctcttttgaa cggatacgtt
     3601 ctcttaaaat atttgcgatt tcttcgtatt ttggcacgtt attcacctct tctagagtat
     3661 aacgaaagat tatgcttccg acaactcgaa gtagcatctg gaacattgtt tattcgaaaa
     3721 aagaggtcat gacaagtttt tgtcacaacc ccttaatgat ctattaagca atatctaaat
     3781 ctggttgttt atctagtact ttcaggaatg ggtaccagat aaaggcaacg atgacaaagt
     3841 cgatgacttg cagcatacca ccaagccatg aatttgttgc taagacaccg cttgcaatga
     3901 ttggcacagt ccatggaaca gagacgccag ttggtgcagg gacaagtcca gcagccatta
     3961 ccaaataatt gaacgtcgtt acgattagag gtgccacaac ccaaggaata aggattgttg
     4021 cattcaatac gatcggtaga ccgaagatcg ctggttcatt tacattgaaa ataccaggtg
     4081 ctaaagctaa tcgtccaaca tcacgatact gtttcttttt aagaacaaag gccatcaata
     4141 aaacgacagc taaagtcatt ccagagccac caataccaac agtgaaggtt tccatgaatg
     4201 gttttgtgat gatgtgtggt aattcttttc catgttgata agcttctaag ttatcaagca
     4261 tcagtgtatt ccaaatcggg tccattacag agttgacgat gatttgtccg tgaagtccga
     4321 agaaccataa gaattggaca aagaacaaag caatcaatgt tgcaggtaaa ctacttccca
     4381 aaccagttaa tggtttttgg atcacttcat aaacgacgtc atgtaagtta gcattgaaaa
     4441 taccagtcat cacagcattg attactaaga aaatagttag tgtcactaca gcagggatca
     4501 aagcggcaaa tgattttgtt actgcaggcg gaactccatc aggcattttg atctgccagc
     4561 cgcgtttcgt gattctgcag tagatttctc cagccaagaa agcagcgatc attccaatga
     4621 acattccttt tgcgccaaga cgatcaagtg aaagcacacc agtcacatct tcaccgccag
     4681 cggttttcat tgcaaaaggc gtaagaatca agaagctggc aaaagacacg gcaccaccga
     4741 aaataccttc aacgtcataa gattttgata aataatagcc gataccaaaa gtaacaaaaa
     4801 ccgacatgat gctcattgtt gcattttgcc cattaccgaa taaattcgat aaagttccct
     4861 ttgttgcgtc actgaaaaaa ggcaagttat tgataacgac aacgatcgaa ccgaacatcg
     4921 ttaaagggaa ggaaagcatg aaagcatccc gcagcactgt aaggtaacga ttttgcccta
     4981 ataaattagc taagggcatg attttctcag aaagcttgtc cataaaaccg ttcattttta
     5041 ttcacctcaa atttatttgc aatcgattac atacaaagta taacgccaaa aattttaaat
     5101 gtctagtcat tttcataaaa agtattgatt tattttttag ggttcgttat gattacggta
     5161 tcaagaaaaa agagaggaag taaaaaaatg aaaacctaca cattcccaga aaatttttgg
     5221 tggggttctg ctgctagcgg accgcaaaca gaaggaagag taagtgatga tggtaaagga
     5281 gaaaatattt gggatcattg gtatgaatta gagcctgaaa aattcttcga tcaagtagga
     5341 cctgaaaaaa cttcgcaagt ttacacaaaa tacaaagaag atgttcagtt aatgaaagaa
     5401 actggccata acacatttcg tacatctatc caatggagtc atttgattcc agaagggaca
     5461 ggtgaaatca atcaaaaagc tgtttctttt tatcgagatt attttactga attgctggat
     5521 aatggtattg aaccttttgt gaacctttac catttcgata tgccactacc actgcaagaa
     5581 aaaggcggat ggttatcaag agaaacggtc gacgcttatg tttcttatgc agcagcgtgc
     5641 tttgaactgt ttggagatct ggtcaaaaaa tggtttactc ataatgaacc aatcgtacct
     5701 gtagaaggag gctacttgta tcaattccac tatccaaatc atatcaatat gaaagaagcg
     5761 gtccaagtag gctatcatga gaatctagca agcgcaaagg taatcaaagc ctaccatgaa
     5821 ggcggacatg atggtgagat cggaatcatc ttgaatctga caccaagcta tccgagagat
     5881 gaaacgaacc cagaagatgt aaaagcggcg aagatcgctg acgcattctt caaccgatca
     5941 ttccttgacc ctgcagtgaa aggagagttt ccacaagagt tgatcgaaat tgtcaaagaa
     6001 ttggctataa tgccagagat ccatgaagga gacctagaac tcatccgtga aaacacgatt
     6061 gacctgctag gaatcaatta ctatcagcct cgtcgaatca aagcaaaaga aactccaatc
     6121 aatcacgaac atagccccat gccagatgat tattttgata attacgacat gcccaataaa
     6181 aaaatgaatc catatcgtgg atgggaaatt tacgaaaaag gaatctatga tattttaacg
     6241 aatacaagag aaaactatgg taatatcaaa tgctatattt ctgaaaatgg gatgggggtc
     6301 gaaggagaag aacgatttgt taacagcgaa ggaattattg aagatgatta ccggatcgag
     6361 tttgttacgg atcatttgaa atacgtccat caagcaatcc aagaaggaac caattgtgtg
     6421 ggttaccaca tgtggacatg tatggacaac tggtcttgga caaatgctta taagaatcgc
     6481 tatggcttta tcgctgttga tttggcacaa gacggtaaac ggacgatcaa aaaatcaggt
     6541 cgctggttta aaacggtctc tgaaaaaaac ggatttgatg cctgatattt tttgataaaa
     6601 cgttcacaaa atgtttgttc taacactgta atcgtgctac actaacagtg aaggaggcgt
     6661 ttacaatggt agatacttat cgtaatattt tagtagcaat tgatggttct gaaaaggcag
     6721 aaaaagcatt tcttgaggca atcacgattg ccaaacgaaa tcaggcaacg ttgcacatcc
     6781 tgtatgtcaa tgaagtaaca ggcaattact ttggtgattt tgcatttgtt acaacaagcc
     6841 ttcaggaaga attggatgag gttgcggaaa accagatgaa agaacatcga aacttggcaa
     6901 ttgaaaaagg cctgacagat atcgagacat atgttcttta cggttatcca aagacactga
     6961 ttgctaattt ccatgaatcg aaagaaccaa tcgatctgat tgtcatgggg gcgacaggtc
     7021 tgaatgcggt agaacgtgca ctagtaggtt ccactacttc ctatgtagtc aaccacgcat
     7081 catgtaacgt catggttgta aaataaataa attaagggaa cagaaagcaa aaaagcataa
     7141 cgcttctttt gctttctgtt ccttttattt ttttgtaaaa aattacttta cctgatgaag
     7201 taatcatgct attctaatta caacgacaga tggagtaata tggaggaaag aaaatgattt
     7261 caagtgatgg aatacgcgga tacaatgatg cgattatttt atcgatcctt aacaaaaacg
     7321 attcttatgg atatgaaatt tctaaaacga ttcgtgagag gacaaatgat tcctatgtga
     7381 tcaaagagac aacactttac tccgtttttg cccgtttaga aaagaatcaa ctgattgttt
     7441 cgtatcctgg agaagtgact catggaaaaa agcggacata ttataaaatc actgaaatag
     7501 ggcgtaatct tttagaagaa aaaaagcaag agtggctgac gacgaaagac gtagtcgatc
     7561 aatttttgga gggaaattaa atggatacaa tccgtaatta tttagacagt ctttttatcg
     7621 gagtacctca aagtaccgag atagataagc tgaaaactga tttgttggca aatatggaag
     7681 accattatca tgaattgatg ggagaaggaa aaaatgaaca ggaggccatc ggtacagtaa
     7741 tttcgacatt tggttcaatc gatgaattat tagaagaact agatgtcgaa aaaaaacatc
     7801 aagctgacga gacggaaaca aacacagctt ctatttattt aagcgaagca gaaaactact
     7861 ggaaagaata cagagcggca agtctccaag tggcatcagg tgttctattc attagcttat
     7921 cttttgcaag ttttcttttc ttttgttcag caggatatgt gttcatgggt atttcttgtc
     7981 tgattttcgg tatagcatta gctgtcggtt tttttattgc ttctggtatg aaaatcacac
     8041 gtttaaatca ctttttgcac catcggaaaa tacctgaaaa agtattagca gaagcaaaag
     8101 aaaaagaaga agagtatcag cgaagttttg gcttctcact gattgcagga attggattat
     8161 gtattttttc acttttccca cttttggctt ctttaatgtg gtatatggat ggtagtatcg
     8221 gtgcctctat attttttgtt acggtaggaa cgggtgtctt cttgatcatt tatggcagtc
     8281 tagtccgtca ttcttatcgt caattcactc aaagcgctta ttactggtga atgtattcag
     8341 atttgtaaaa atattctatt tatccggcgt agaaaaaaag actgtgacac gcttttgtca
     8401 cagtcttttt tatatataat aatttaaaaa ttcatcatct aaatccgtag aattttctag
     8461 attgcttacc catgtaaaat cgttatagac agatttcagt tgacttctgc gccattgtac
     8521 agggataaaa agacgcatgc tatcttcatt gaatccgact aataatttct ttccgtcagt
     8581 aatgatggga cgttgtaaca aagtaaaatg cgagacgatc agatcgacaa gctctgaggt
     8641 cgtacattgg tcgatttggt ctttcaaatt tttataatca atcgagcgtt tagctaatag
     8701 atcgtcaaat ccgttttctg ttaatgacaa taaataaaat atctctttcc ttgttaaagg
     8761 ctgtttaggt gtgaaaattc gttctctgta aggcatttga tggtctttca gccagtttgc
     8821 catcagatgg taactatgtg tgcgagaaga atagatcgta agcataaaaa aactccttaa
     8881 atagtccatt aatttgttag atctgttgag ataaataata ggtaaggagc tgtgcataag
     8941 cattgatcaa aacaacccaa acatcattag aaataacttt ctaaagtctt aaacttgcga
     9001 cgagaaacaa ctagactaat agaaaaaatg tcttagccat ttggcttcct gtttgttttt
     9061 ttgcttatgc tgcagctcct cgggaaaggg ttctaacaat ttgaagaagg ttgatgattg
     9121 atctagttta aattgtgtga aatttgaaat ctggtgtttt tactagatga atcacctaac
     9181 aactatgatt ataacttata gaacttctgt tttgcattcg gttttgttat agttgttata
     9241 aagttttatt ttattacaga acatttttaa ttaataaaga acagttagaa gttaaaattg
     9301 ctgattcaca tagagtttat gacttcttgg aaattctttt tcaatcagtt ttttcatctg
     9361 tttatgaaat tctttttttt cagtaaacat aaaggtttgg gtatcgtaac cttcttcatg
     9421 taggaaatag attattcctt tttctgtgtt ttgcgt
//
LOCUS       DACEND010000100         9294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEND010000100 DACEND010000000
VERSION     DACEND010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.103"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1443)
                     /gene="nhaC"
                     /locus_tag="IW746_002044"
                     /pseudo
     CDS             complement(75..1443)
                     /gene="nhaC"
                     /locus_tag="IW746_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(1552..2973)
                     /locus_tag="IW746_002045"
     CDS             complement(1552..2973)
                     /locus_tag="IW746_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1078938.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IW746_002046"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IW746_002046"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1078939.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="IW746_002047"
     CDS             complement(5384..6640)
                     /locus_tag="IW746_002047"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1078940.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="IW746_002048"
     CDS             7220..8521
                     /locus_tag="IW746_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1078941.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="IW746_002049"
     CDS             complement(8590..9189)
                     /locus_tag="IW746_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1078942.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2802 a         1925 c         1651 g         2916 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc caaataaaat
      721 aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat
      781 ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat
      841 caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag
      901 attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt
      961 ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa
     1021 atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac
     1081 agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac
     1141 aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac
     1201 taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat
     1261 gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca
     1321 catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc
     1381 taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt
     1441 catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc
     1501 aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttcc
//
LOCUS       DACEND010000101         9211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEND010000101 DACEND010000000
VERSION     DACEND010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.104"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..789
                     /locus_tag="IW746_002050"
     CDS             52..789
                     /locus_tag="IW746_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1078943.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="IW746_002051"
     CDS             817..1356
                     /locus_tag="IW746_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1078944.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1854..2315)
                     /locus_tag="IW746_002052"
     CDS             complement(1854..2315)
                     /locus_tag="IW746_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1078945.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            2448..2822
                     /locus_tag="IW746_002053"
     CDS             2448..2822
                     /locus_tag="IW746_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1078946.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2876..5002
                     /locus_tag="IW746_002054"
     CDS             2876..5002
                     /locus_tag="IW746_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078947.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            4999..5619
                     /locus_tag="IW746_002055"
     CDS             4999..5619
                     /locus_tag="IW746_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078948.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            6312..6692
                     /locus_tag="IW746_002056"
     CDS             6312..6692
                     /locus_tag="IW746_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1078949.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            6674..7588
                     /locus_tag="IW746_002057"
     CDS             6674..7588
                     /locus_tag="IW746_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1078950.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            7828..8517
                     /locus_tag="IW746_002058"
     CDS             7828..8517
                     /locus_tag="IW746_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078951.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(8637..8723)
                     /locus_tag="IW746_002059"
     CDS             complement(8637..8723)
                     /locus_tag="IW746_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1078952.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         3564 a         1402 c         1423 g         2822 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
     1621 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
     1681 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
     1741 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     1801 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
     1861 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
     1921 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
     1981 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
     2041 aaacctaata tagtaacaaa tatattaaaa ataatagctg tcaataccag taaattattt
     2101 tttttatttt ttgaattatc ttcatttaaa atagaattta tgttagtact ataaaattca
     2161 caaatttttt ttagtgtctc tatatctggt gttgttttgt tattttccca ctttgaaata
     2221 gtttgacgtg taacgttaaa ttttcttgcc acatattctt gagtataccc ttttttcact
     2281 cttaattctc ttagcttaga tcctaaatcc tccatttttt ctcctatgta aacgattatt
     2341 tacataaaat tttatcataa aaatactaac gcgccaaaaa agttctttat tttataaaac
     2401 acactaaaat aaaataattc tagtaaaaaa gataggagaa ataagttatg aaaaaatttt
     2461 tatgtctttc tgctttaatg ggtgtcttat taactagtgg aggaatagct agtgccacag
     2521 aagctttaaa tctagatgta cccgaagacc ataccgccat ttacggagga ggaatggaag
     2581 ctacagaaga aggattttct tctagtaaat tgagatatgc tgctggcggc ggggatttca
     2641 actgtggggt taatggattt aaagtttatg caaattacta ccatgctaga gctaaacata
     2701 gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc agttcagaaa gctggagtaa
     2761 gagcttatgc aacatgcaat gccacactta ctggaaatac tggttggtgg aatgtttatt
     2821 aaaaaagaga gtttaaactc tcttttttat ttacatttga aaggaaattc aaaatatgtt
     2881 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
     2941 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
     3001 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
     3061 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
     3121 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
     3181 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
     3241 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
     3301 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
     3361 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
     3421 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
     3481 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
     3541 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
     3601 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
     3661 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
     3721 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
     3781 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
     3841 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     3901 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     3961 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     4021 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     4081 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     4141 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     4201 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     4261 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     4321 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     4381 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     4441 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     4501 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     4561 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     4621 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     4681 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     4741 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     4801 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     4861 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     4921 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     4981 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     5041 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     5101 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     5161 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     5221 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     5281 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     5341 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     5401 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     5461 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     5521 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     5581 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     5641 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     5701 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     5761 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     5821 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     5881 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     5941 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     6001 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     6061 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     6121 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     6181 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     6241 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     6301 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     6361 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     6421 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     6481 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     6541 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     6601 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     6661 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     6721 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     6781 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     6841 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     6901 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     6961 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     7021 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     7081 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     7141 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     7201 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     7261 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     7321 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     7381 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     7441 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     7501 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     7561 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     7621 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     7681 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     7741 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     7801 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     7861 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     7921 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     7981 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     8041 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     8101 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     8161 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     8221 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     8281 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     8341 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     8401 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     8461 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     8521 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     8581 ccagagaggt cggattctct ggggcttttt tcaggttgat gtcgccttta ttgattttac
     8641 ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaactcc tacaagaagg
     8701 gggcaaatga acagttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata
     8761 ggcgacaagt tattatatct taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg
     8821 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gcttctcgac tagattaagg
     8881 gaaattaatc aagaattgtc ttagaacggc ttagacaagc tttcagagtc tcacacctcc
     8941 tctttgaaca acggttttag caaaggagcc ggaggcgact gcgcagtata atatgagagc
     9001 atagcgatca caccttttta gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg
     9061 cgaacctttt tggaggaaaa agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa
     9121 gatatactgt tagatatact gttagatata ctgttaataa taactgttag ggattagcaa
     9181 ccttatggga gagtaagtat tgacataggt a
//
LOCUS       DACEND010000102         9118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEND010000102 DACEND010000000
VERSION     DACEND010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.105"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            34..1368
                     /locus_tag="IW746_002060"
     CDS             34..1368
                     /locus_tag="IW746_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078953.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..2983
                     /locus_tag="IW746_002061"
     CDS             1355..2983
                     /locus_tag="IW746_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078954.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3158..3991
                     /gene="msrA"
                     /locus_tag="IW746_002062"
     CDS             3158..3991
                     /gene="msrA"
                     /locus_tag="IW746_002062"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1078955.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4071..5060
                     /locus_tag="IW746_002063"
     CDS             4071..5060
                     /locus_tag="IW746_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1078956.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5349..6047
                     /locus_tag="IW746_002064"
     CDS             5349..6047
                     /locus_tag="IW746_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1078957.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6197..7513
                     /locus_tag="IW746_002065"
     CDS             6197..7513
                     /locus_tag="IW746_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078958.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7530..8945
                     /locus_tag="IW746_002066"
     CDS             7530..8945
                     /locus_tag="IW746_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1078959.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         3083 a         1420 c         1815 g         2800 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt attcgtttgg tttctaaagg
     1981 tttcaagaat gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac
     2041 attggttctg acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat
     2101 caacaaacct agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac
     2161 tgaagctatg aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt
     2221 atgtattccc ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg
     2281 tacgtgtttg acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac
     2341 gccagccaaa ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctcttc
     2401 tctgatatta acgatgatat ttttcaaaaa tagaagatac aataagacaa gagaactatt
     2461 ttatttattt atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt
     2521 caatcaaaaa ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc
     2581 gtataccact aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga
     2641 gaataaggag catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc
     2701 ttataaaaca actggtagtt ctatcatata tagagtaact aatgagaaga gccaagtaat
     2761 cacacttcct gtatatgctt atttaggaac gaatgtagaa ataaatcatc aacctgtaaa
     2821 ggagcaggtg aatgctaatg gattggtaca aataagcgga gtcaaaggag ttgctgatat
     2881 taaagtaagc acaagttata cagtaacgac tattatttct tatcttgtta cattaactgc
     2941 atatttatgt ttatttttta aaatagtttt ccataaaagg taaattaact tttcatttat
     3001 aaaataaatt gattaaaaag gagaatctgt cgataaggat tctctctttt ttatacgaaa
     3061 aatctgtagg gggatgaagt aatcgcttat ttcctcactt tatgataaat tacagctaaa
     3121 gtcactagct tgtcttcttc ggaaaggagg agaatctatg gatgaggaac aaatattcat
     3181 tcaaacactt tataatctga ttttgaatcc gaatacacga gattgggaac gaaaagtatt
     3241 gattcagaca aagaatgaca taagagaaaa tatatccgtt aaagaacagt tatcaaaact
     3301 agaagcaaca ctacgtccat tagctatccg aatgaatctg acgcctgatg taatggactt
     3361 ttatttattg attacggaag gatttgataa ggagcaaaaa tatgactttt ccaagcatgc
     3421 catgcaagat gctgactatc aagaacgtgc agtatttgca gggggatgtt tctggtgtat
     3481 ggtcgagccg tttgaatcga aaaaagggat cctatctgtt ttgtcaggat acacgggcgg
     3541 acatgttgaa aaaccaaatt atgatcaagt aagtggtggt tacacaggtc atgtggaagc
     3601 tgtggagatc atttatgaca caagagaaat aagttatagc gagttgctga caatctattg
     3661 gcaaattact gatcccacag atacatttgg acaatttcaa gatagaggga agcaatatcg
     3721 tccagtgatt ttttatcaag gcgaacgaca aaaagaatta gcggaacaat caaaacagaa
     3781 actagacagt tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac cagccggtac
     3841 gttttggcca gcagaaaact atcatcagca attttacaaa aaacaaccga aacgttataa
     3901 aaaaatccag caagcacgta atcaatttct gatctatcaa cgagtaaaaa acaaatggca
     3961 aaaaaatata cgaaaaaatc attttgatta actccttttc tttaaaatgt agtaaactaa
     4021 aaatagaaaa aagaaagcgt tttaaaaaga tttgatgagg aggagaaaaa ttgtatattg
     4081 cagatagtgc acgttatgaa aaaatggaat atcgtaaatc cggtcattca ggtttgaaat
     4141 taccgatttt atctctcgga ttatggcaaa attttggtga ttatgatcct atccataatc
     4201 aaagagagat tttaagaggc gcattcgaca tgggaattac ccatttcgac cttgcgaata
     4261 attatggagg ccctgctgga gctgcagaga aaaactttgg gcgtattttt agagaagatt
     4321 ttcaagccta ccgtgatgaa ctgattattt ctagcaaagc aggttatcat atgtgggaag
     4381 ggccatatgg ggaatggggc tctcgtaaat ccatcatttc aagttgtgac caaagtctcc
     4441 aaagaatggg gctagattat gttgacatct tctatcatca tcgcccagac cctgatacac
     4501 ctttggaaga aacagcagaa gctttgatgc aacttgtaag acaaggaaaa gcattatata
     4561 ttggtatatc caactataat ggagaagata caaaaaaaat gactgaaatt ttaaaaagaa
     4621 aggaagcacc gttcattatt catcagatgc gctacaatat gttttctcgt gcactactag
     4681 aagacgatct atctccagta ttagaagaag aagggctagg ggcaatcacg ttcagtccgc
     4741 ttgctcaagg gttgctgaca aatcgttatc tacatggtat tcctgaagat tctagagcac
     4801 atcgtaagga aataccattc ctttcagaag aacaagttgg atcaactctg gaaaaaatca
     4861 aagcactgca aacaatcgcc gtatcacgag gacaatcatt agcgcaaatg gcccttgcat
     4921 ggaatttgcg acaaaaatct gttacaagtg tactggtcgg agcaagtcgg ttaagtcagt
     4981 tacaagaaag tgttcgaatg atggataatt tagatttctc accagaagaa gaattgagaa
     5041 tcgaccaaat tctagaatag aaacgatgta atcaaacttt ttatccatat attaaaaaaa
     5101 acaaaagatg acatcaagaa tcgaatgaag atcgtctgtt attgaacatc atattttttg
     5161 tcgttcaaaa taaaaatgat cgaaggctag gagtgaattt tatcctagtc ttttttatgg
     5221 taaatatctt cttcgtttgt atgtctatga acggattaat caattttgta tacaaaaaat
     5281 catcagatga ttcaaacgtg ttataataat tacgaaaaaa tacatcagat gaaaaggagg
     5341 tatgtggttt ggaagaaaat aaaaccctag tagaagtaac tactgacaag atccttagat
     5401 atttaaagga aatgcagttg caagtaggag ataaactgcc gaatgaatat gaattgtccg
     5461 attatttaga ggtggggcga agcacggtgc gagaagctgt gcgggctcta gcttccagga
     5521 atatccttga agttcggcaa ggagcgggga catttgtcag tgataagcga gggattgcca
     5581 atgacccact cggtttttct ttgattgaag atcaggatcg gatgatacga gacttatttg
     5641 aactgagata tttattggag cctaagatgg caggtttggc agcggaatat gcaacgaaga
     5701 atcaaatcgt ccaaatgaaa aaattaacgc aaaagattga aacttctttt cgagaaggca
     5761 cacaagaaca tgttcaatta gacatcgatt ttcatacgat gattgcagaa gctagcggaa
     5821 acgtagcctt gctgcatatt atcccaatca ttaatgaatc aattgctttg tttaataaaa
     5881 attatgatat ccagcatctg aaaatcgaga cgatcgatat tcatacagag atcgttcagg
     5941 ccatcgaaag gcggaatgca cttgctgcgt ttgatgcaat gactgtccat atggcaataa
     6001 atcggagaga attacagaaa ataataggag aaaaagaaac taattgacag ttcatctgat
     6061 gtattttatg ataaatacat cagatgaatt tttgttttat atttggtaaa aaagaggagt
     6121 aaatagtttc tgttcatcga aatgtaagcg ctgactaata gacaaaaaga gatgacgtca
     6181 aggagaagaa gcaggaatga ggggatctgg aatgagtttt atggataaat tagccaatgt
     6241 tttagggaac gtggcaacga gaatcaataa tttaaggtat atcatggtga taaaaaacgc
     6301 atttgctgca ttgattccgg taattattac gggtgctttt ggcacattgt tttcagcaat
     6361 ggtctttgac tcggaaaatg gcttagcaca gataagtttc ttgcgttttt tagaagaatt
     6421 aaagcctatc gcatctgcca tcagctatgt cacactaagt ttcttgacca tctatgctgt
     6481 ttttctgata gggatcgaat tagccaagct gaataaagtg gatggtgtat tccctggtat
     6541 catcgcagtg atgtcttatt tatctgttaa tccaactgtt tatgaattag tgaatgaaaa
     6601 tcagactgtt atagtagaaa acgtattggc taaacaatat acagatacga aagggttatt
     6661 tttgggaatg ttggtctcca tcctttctat tgaactttat tgctggttga ggaaacagaa
     6721 gaaactacag ctgagaatgc ctgataccgt gccaacgaat gtttctgcta gtttttctgc
     6781 tttattcccg acgattctga ctgtatcggg tatcgcggct gcgggattta tcatcaaaga
     6841 acttacagga atgtaccttt atgatatcat ttacaatgtc gtacaacgcc cattagaagg
     6901 tgtcgtacaa gggttaccgg gtattttgct attgatgttt attgcgcaag tcttttgggt
     6961 gattggtatc catggaaatc aaatggtgaa gccgatacgg gaaccgttgc ttttagcggc
     7021 aattgctgta aatacggaag cttttgaagc tggaaaagaa gtaccgaaca ttattactat
     7081 gccattttgg gatatgtata tgagtatggg cgggtctgga gtcacgatcg gtttactgat
     7141 tgcagtattg ttagttggaa aacgggaaga tatgaaacaa atcaccaagt tatcccttgc
     7201 tcctgggatc ttcaacatca atgaaccagt tatttttggt atgccgatca tgctgaatcc
     7261 aattttagct attccgttta tcttaacgcc tcttatcact ggaacgattg gttattttgc
     7321 aaccgcttta ggatttgcgg cgaaagcagt tgtaatggtc ccttggccaa tgccgccgat
     7381 cgtcaatgct tatctggcaa cagcaggaaa tatcggtgca gttattacgc aacttgtttg
     7441 tattattgtt tctattttga tctatctgcc atttgtaaaa gtagcaaatc gtgtggaaaa
     7501 tgatgggatc taaaaaacag gaggaaatca tgaaaattac tattccaaaa aattttattc
     7561 ttggtgctgc atcttctgct tggcaaacgg aaggttggaa aggaaaaaaa gaaggacagg
     7621 attcgtttat agatagctgg tataaaaatg aacattttgt ttggcataat gggtatggcc
     7681 cagccgtagc aactaatttt atggaacaat accagcagga tgtggatctg atgaaatcca
     7741 tccatctgac acattatcgg acttctatca attgggctcg tttttttact gattatgaga
     7801 actgtatagt agatgaagaa tatgctcagc atataagtga aatgatcgac tctttgatcg
     7861 aagcagacgt agaaccgatg atttgtttgg aacattatga agtcccagct tttcttttgg
     7921 aaaaatatga tggctggtct tcaagaaaag tagttgattt ttatgctaag tatgctcaaa
     7981 ttgcctttga tcgttacggt gatagggtaa aaaactggtt tacattcaac gaaccaatcg
     8041 ttccccaaac gcgttgctat ttggatgctg ttcgctggcc gcatgaacaa aacacaaaaa
     8101 agtggatgat gtggaattat cataaagttt tagcaacggc taaagcagta gagatttacc
     8161 gcagcggaaa ttatggtgga aggattggct gtattttaaa tcctgaaatg gtctatgctc
     8221 gatccaattc tccagaagac caaaaagctg cttatatgta tgatttgttt tataatcgag
     8281 tattttttga tccgatggta aaaggatact acacagaaga gttcctggag ttgtgtcaaa
     8341 aacaatcgat ttatttcgat cctgcaacag aggatttaga aacgattcag catcatacag
     8401 tagatttcct tggaatcaat caatattatc ctaaacgcgt tcgcgcccct cgttatcaat
     8461 ggaatgaaga gacacctttc catcctgaac gatattatga agtctttgat ttacctggga
     8521 agaaaatgaa taattccagg ggatgggaaa tctatccgaa aatcatgtat gatatagcca
     8581 tgtatctaaa agaaaattat cataatatcc cttggctgat aacagaaaac ggtatgggaa
     8641 gagaatatga agaacagtat atggatgaga aaggaatcgt gcaagatgat taccgtatcg
     8701 aatttatcgg acagcatttg cattacttga tcaaagcaac agaagaaggc gcaaattgcg
     8761 aaggctatat gttatgggca ttcactgact gtgtgtcacc aatgaatgca tttaagaatc
     8821 gatatggatt agttcgaatc gaattgaacg atgaacgaag cagaacattg aaaaaatctg
     8881 cttcgtggta tcagactttg attgataaga gagaattgga tttgcctgac gaaatctata
     8941 aataaataga agttttttgt agatgttcat tgtatttact ggttatttga aataaaagag
     9001 gttgtgaatc cagcgttttg agctaatacc cagggtcagc tgattgaaca ccgtttattg
     9061 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa tagcaaagta aaaacaac
//
LOCUS       DACEND010000103         8759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEND010000103 DACEND010000000
VERSION     DACEND010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.106"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW746_002067"
     CDS             complement(<1..182)
                     /locus_tag="IW746_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078960.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPIL"
     gene            complement(479..2860)
                     /locus_tag="IW746_002068"
     CDS             complement(479..2860)
                     /locus_tag="IW746_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1078961.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2850..3497)
                     /gene="recU"
                     /locus_tag="IW746_002069"
     CDS             complement(2850..3497)
                     /gene="recU"
                     /locus_tag="IW746_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1078962.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3561..4100
                     /locus_tag="IW746_002070"
     CDS             3561..4100
                     /locus_tag="IW746_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1078963.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4190..4609
                     /gene="gpsB"
                     /locus_tag="IW746_002071"
     CDS             4190..4609
                     /gene="gpsB"
                     /locus_tag="IW746_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1078964.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4631..4998
                     /gene="rnpB"
                     /locus_tag="IW746_002072"
     ncRNA           4631..4998
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW746_002072"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5207..6373
                     /locus_tag="IW746_002073"
     CDS             5207..6373
                     /locus_tag="IW746_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1078965.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6419..7915
                     /locus_tag="IW746_002074"
     CDS             6419..7915
                     /locus_tag="IW746_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1078966.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7996..8679
                     /locus_tag="IW746_002075"
     CDS             7996..8679
                     /locus_tag="IW746_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1078967.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2779 a         1569 c         1840 g         2571 t
ORIGIN      
        1 aggataggag caattaaagt aaatttattt aataataaac aaaatttttc agctttttta
       61 ttcttggaat aacgaggtat tgttttcgat aaaaaaagat ggtgcagaca aattatgata
      121 attaagcata ctatcagata actcaaaatg aagccttctt tcaaaaaatt attcaaaacc
      181 atatattgta ttttttattt gataataaca atgggattat taagaagtgg cactaaataa
      241 aagactagtg tgtatttagc cgatttaatt ataaacaagt aaatggataa agtatatgta
      301 atgataaaag aaaaatggat tttctaattt ttaagctaaa tgttagatca cacatgtata
      361 tgctggatag tcttataaaa aatcatgtaa tacagcttgg tacatgattt ttatttgtag
      421 ctgtttttta catagacaca aaaaagaaac cacgcttgta gacgtggttt cttttttact
      481 atctgtttcc agaagctgag gtgctgctgg atggtgtatg gtcatttgca gcatccgctc
      541 cgctagttgc tggctcactg gaagaagcag gaggagcaga tgaagacggt gtcgagcttt
      601 cttcggatga agaagaagga accgttgttg aagacgctgg ctcgcttgtt tctgctgtcg
      661 tgctttcttt tgaggactca gcagttgatt cgctttgact agtagagcta gagctttgag
      721 tcgttgtctc tgttgttgag cttccaggag tctggacata gctggaagat ggaatagttg
      781 ttgatggtgt aattgcattc gatctagctg tatattgatc tttgtagtac agttcaccgc
      841 ccacacgaat taatccgctt ggcatctccc agtctgtatt tgtcacgtta gctgaaacat
      901 actgcataag ctcgcgataa acatcagagg ctacgtgtga cgattctgaa gtgacaggtg
      961 tcattttttt attgtaacct gtccacacgg aaatcgaata atttggcgta tagcccgcaa
     1021 ataggatatc tggatacacc ccagaagaaa taccaagttt tgcatactcg tcatctgtgt
     1081 aattggatgt tccggtctta ccggcttgat ataacccagc gatttgcgca ttcgtaccag
     1141 ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat ataagctgtt tctggagaca
     1201 tggctgtttt tccatctggc tcataagttt cttctgttcc gtcttggaag acgattttat
     1261 taacatattg aggtttgtaa taagtccctc cattggcgaa agccgcatag gctgctgcca
     1321 tttttaagga agaagctccg tattttgtcc catcttgttc ttccgtatta ctagagatcg
     1381 cattcgattg atggatcgta ctgtattcga tcccaagatt ttttaagaaa gaagccactt
     1441 tatctgaacc gacttcattg aataatttga ctgctggaac attacgggat aagtaaagtg
     1501 cttggcgtaa agtaatggtg cccatatatt gattatccca gtttcctaca ggagtcgaag
     1561 tcccttcata gttatacggt gcgtcagtaa tagtttttcc agtagaatat ttcaaatatt
     1621 caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga accaaaatca cgggaagtat
     1681 tcactgctaa gttgttgcct aaagttacgt cttctgcgat gtgacgtccg ccaatctggg
     1741 cttttacttt cccggtattg gtgtcgataa gggtggaggc aacttgcatc tcgtcgtctg
     1801 ggtaagagac atactgatcc gtgttgacga tatcatatag tttcttttgg gcgtccagat
     1861 ccaaattcgt ataaatctct aaaccgtctg tgtagacatt cttatctgtt ttttcttgta
     1921 cttcattgat gacttctttg acataattat cgacgatctt agtattgtca tcggattgag
     1981 ttagttcttg cagaccatcc gttaccggta cattaacagc ttgatcatat tctgtttgag
     2041 agattttttc gttttgaagc atcgtataca aaacagtatc tcgtcttttc ttcgcctgat
     2101 ctggataaac ataaggatca taggcggaag gggcttgagg catcccagca agcaatgctg
     2161 tttgcggtaa agaaagttct gataatttct taccaaagta catctcagaa gctgtctcca
     2221 tcccgtacaa gccgtttgac atatacactt tatttacgta gtaggtcaag atctcttgtt
     2281 tcgatttttt ttgttccaat cttacagcca tccacgcttc ttgggccttg cgtttcagag
     2341 tctgatcctc ggcactggtg gagaagaagg aaagtttgat caattgctgt gtcaaagtac
     2401 ttccgccttg cagtccgcca gaggtaaagt tagagagggc agaaccgatg atccgaatag
     2461 ggtcaacccc gatgtgttta taaaaacggc gatcttctac cgacacgatc gcatcttcta
     2521 atgttttcgg taattcattc gcagaaattt tttcacgctt ttctgcgcct aaatcttcga
     2581 atagttcgcc atcttgtgta tatagtttgg aagatacggt agcgtctaat tttttatccg
     2641 tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc tacacctgct aaaaagagga
     2701 tacagaaaaa tgagagtatt cctaaaagga tcttgataaa taatcctttt ttatgtgttc
     2761 cagaggactt tccagaacct ttgccagaat tcttcttcac actcttttta ggagtaggct
     2821 gtttctgtcg tctagagctt ctagtttgtt catttgccat tcttgctttc tccttcaata
     2881 gataatgttg ataaatagaa gtcaaccgcg tccaaataag gaatccgcgg agaaatacca
     2941 gttttgatct ctattcctag ttcttcgatc aaagataaag gtagtgattt gcgtcctttt
     3001 tccttttggg cgtcccagta agcaatcaaa tgaacactat tcaaataaaa acagcgcttc
     3061 aaagacgaaa accataacaa gacaaagcag accccatttt gttcgaggca tgctttcata
     3121 tgttcgattt gatgggcatg gaagttttta aagggaaaag atgttttgtt cttggtttct
     3181 tttgcttcaa agtccaagta aaagcctcga tagacgccat tatagtctgt agttgaggct
     3241 tgggtaaagt aggcttcctt gatcacagca gcacttctct taggatagtc tactttcacg
     3301 atttggatag gggtaggctt tttatggata accgccaacc cgcgggaaag ataatattta
     3361 ttactttcat tgattgcttc ttcaaagcgc atcccccgat tgccgaattc agtgatctga
     3421 tttctttttt cttgaatttt ttcgacggga gaggcattct tataataagg ttggccattt
     3481 ggatagcgca ataccatctt taatcacact cctaggccac attatagcaa aaaagcggat
     3541 agaaagaaaa ggaatctgag atggataagt caaaagtaat ctatgtgtct gggtatcgaa
     3601 gtttcgaact cggtgttttt aaagaaaatg atcctaaaat ccaagttgtt aaaaatgttt
     3661 taaaaaaaga actccatcag ttagcagaag aagggctgga atgggtactt gtcagtggga
     3721 atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga attgaaagaa gaatatccgg
     3781 aactatctct tggtattttg tatccttttt ctgattttgg taataactgg aatgaaggga
     3841 accaagaaaa aaaacaatta gcagaacaat tagcagatta tgtcgaagct gttagccatc
     3901 aaccctacca atcacctagt cagctaaaga atcacactcg ttttatactt gagcatacag
     3961 tgggctgtct gctgatctat gatgaagaat tccctggaaa aacgaagtat ttttttgaag
     4021 atgcacagaa ttaccaagca gatcatccct acgaaattcg gctgatcagt atggacgatc
     4081 ttcaaaattt tagtgaataa tcgatattgt gtttgattta tcaacttttt tcctatacaa
     4141 tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga ggtgtaaata tggcgaattt
     4201 ggtttatagt cctaaagaca ttttacaaca agaattcaaa acaaaaatgc gcggctatga
     4261 tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa gattatgaaa cttacagcaa
     4321 agaactcctt gctttacaag aagaaaacga tcgattgagt gcaaaagtag ctcaattatc
     4381 taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca gaggcaccaa agagcgcagc
     4441 agtgaccaat tttgatatct taaaacgctt gtcaaatctt gaacgtgaag tatttggtaa
     4501 aaagcttgat caacaagcaa gtgcagtcaa accagcacaa cctaatccaa ataactacac
     4561 aaatgcagac acaagcttag atgataatga aaaaacacgt caattttaat ttttgattaa
     4621 tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc aggctgagga aagtccatgc
     4681 tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga aaccataagc taaggtactc
     4741 tttggagtaa cggcaggaaa acaagctaag gcttcggcta tgcttaagta tccttgaaag
     4801 tgccacagtg acgaagcggc tggggaaacc catccggtgg aacgcggtaa acccctcgag
     4861 cgagcaaccc aaacaatggt aggggcgctt tttgtctgga agaagaacag gacaaaaagg
     4921 cagtataact gcagatagat ggttaccgct ttgttttttc tccctggaaa agcaaagtac
     4981 agaacatggc ttatagaaaa ccgcaaacat cagggaatta ggtcgtgaca aaagtaatgc
     5041 gttataagaa acaagaaaaa catttgaaag ctatttttca atacttcaga tgaatcgttc
     5101 ttattacaaa acaaactttt gtatcaccgg ttattcggaa tttgagtaaa agtgtggggt
     5161 aggactgatg tcccacctcc tttttttata aataaggaga ttgtgtatgg aaaaaaacag
     5221 gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa gcactggttg gtaaagaatt
     5281 aagagatctt ggaatcgaat gccaagttga gaacggacgt gcacgttttg aaggaacaat
     5341 ggaaacgatc gctacagcta atttatggtt acgtacagca gatcgagtaa aaatcgtggt
     5401 tggggaattc gatgcgtata cgtttgatga attgtttgaa aacgtaaaag cattgccttg
     5461 ggaagatttt cttccactag atgcagcttt tcctgtagct ggaaaatcga tcaagtcgaa
     5521 actatatagc acacctgatt gccaagcgat cacaaaaaaa gcaatcgttg aacgtctccg
     5581 tacctattat caccgtccag cttctgtacc actgacggaa actggcgcac attttcagtt
     5641 ggaagtagct ttgctgaaag accatgtgat ggtaactctg gatacgacag gaccaagttt
     5701 attcaaacgt ggttatcgct tagaaaaagg tggagcacca ttgaaagaaa acatggcagc
     5761 agcacttatt atgttgacaa attggcgaaa agaccgtcca ttttatgatc ctgtatgtgg
     5821 ttcaggtacg ctttgtattg aagcagcctt aataggccat aatatcgcac caggattcaa
     5881 tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa gaaatcatgg aaaatgttcg
     5941 atcagcagcc gaagaaaaag cagattatga tattgaatta gatattactg gttcagatat
     6001 caatggacgg atgattgaaa ttgccaaagc caatgcagaa gaaatcggat taggctcttc
     6061 gattaccttt aagcaacaag cagtcaaaga cttcaaaacg gataaagaat acggggtcat
     6121 cgtggctaat ccgccttacg gagaacgtct aggagaagaa gaaactgttc ggaaattata
     6181 taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg agtaaatata tcttgactag
     6241 cgacttagcg tttgaggaat tttacggaca aaaagcaacc aaaaaaagaa aattgtacaa
     6301 tggtgcattg cgtacggatc tattccagta ttggggaact cgtccgccta gaaaaaaaga
     6361 gaaagacgaa taatcttact aattgattca ttcaattgga tgtttggagg aatagggaat
     6421 gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat cttctacaac aagctttagg
     6481 aatcctcgat tgggatacac aaacaggtat gcctgaaaaa gctagtgcat accgaagtga
     6541 agtagacagc tatctttaca gcatttattt ttcaaaaaaa atcggtccaa aaatcaaaga
     6601 agccattcgc tatttttctg aacatccgga agaattgtcg gaagttggaa ctgccgtttt
     6661 tgccaaagta aaagaagagt atgatttgga acatcgcgta ccggaagatc ttatgagaaa
     6721 atatacagct gccgtttctt cagcacacgg acaatggcaa aaagctagag aaacacaaca
     6781 attttctgat tttcaacacg cattgaatca aaatattgaa ttaacaaaac agttgatacc
     6841 ttactggaga aaagatgaaa agacgaatta tgatgttttg ctgaatcaat acgaacccgg
     6901 gatgactgtc gaaatccttg ataaggtatt tgatcagctt aaagaaggaa ttctttcgat
     6961 ccgccgtacg ctccaagaaa aagggactga acctgaaaca gactttttaa atcggagaat
     7021 gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc gaacaacttg gttatgattt
     7081 tagtcgtggt cgtttagatg acactgtcca cccatttatg actggaatta atccgaatga
     7141 tgcacgaatc acgacacgat ggaacgaaac agatttcaaa atggctgttt ttggagtgat
     7201 ccacgaggct ggtcatggta tgtatgaaca agacatcgat gataaatatg cgtatacacc
     7261 ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa tcgttgttta acgagatcat
     7321 tattggaagt agtcgcagct tctggaaaaa gcaatatcct ttctttcaag aatgtgcaga
     7381 aggaacattc gatgatattc cttttgacaa attttatcgt tcattgaacc atacaaaatc
     7441 aagtttggtt cgtatcgagg cagacagttt gacttaccct ctccatatca tcattcgtta
     7501 tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt gctgaactac ctgaaatatg
     7561 gaatcaaaaa tatgaagagt atctgggaat acgaccaaca aatgatgtgg aaggcatcct
     7621 acaagacgtt cattggtcag gtgcaagctt tggttatttt ccctcttatg cattagggta
     7681 catgtatgcg gcacaattac ttcattcttt gcagcaagaa atagatgtgg aaaatgttct
     7741 agcgtcagac gattattctc caatcaaaaa ctggatgtca gagcatatcc atcaatatgg
     7801 tgcatctaga aaaccaaatc aattaatcat ggatgcaaca catgaaccgc ttaatccgca
     7861 atacctgatc gactatatga aagacctgta ttttaatgta tatcaggtaa aataaaatac
     7921 aatttacaaa gatagctgaa attcatctaa tctatagatg ttttcagcta tttacttaag
     7981 aaagaagtgg aacgaatgga taaattacaa gtgatcgctg attatagtat ggaccaatta
     8041 gcgcatgatc aaacaggaca tgggagcgat cataccaaaa gagttgtcaa gttagctgaa
     8101 aaaattttgg agacggaacc gcaggcagac cgctttgtga cgcttgcagc agcttaccta
     8161 catgatacga ttgatgataa agtcgttaaa aatgagaacg aggcaaagca acagcttcgt
     8221 gtattccttc gctctttgcc aatagtagag gaacagatct cgatgatttt ttccattatc
     8281 gaaaatatgt ccttttctaa aaatctctct gaagcggccg aactatctct ggaaggtaag
     8341 atcgttcaag acgctgatcg tatcgaagca ttaggagcaa tcggcatttt gcgtactgct
     8401 tatttcggcg gtggacatgg gcacccgatt tttgatcctg aactgtatcc gcaaacattc
     8461 aaggacaaag aaaattaccg aaaaggcacg acagtgatca atcattttta tgaaaaactg
     8521 ttcttgctgt ctgataaaat gaatactgat tacggatatg aagaagccaa acgaagagaa
     8581 accttcatgc gtgaattctt agaagaattt tttctggaat ggtctattga cgatgaaaat
     8641 tttactccag acaattggat caaaaataac aaaaaatgat ggaggttgtg acagaagtgg
     8701 tcagctccaa gatatttcgg atcattttcc gtaaaagtta cttctggaac actgtttac
//
LOCUS       DACEND010000104         8680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEND010000104 DACEND010000000
VERSION     DACEND010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.107"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..407
                     /locus_tag="IW746_002076"
     CDS             <1..407
                     /locus_tag="IW746_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1078968.1"
                     /translation="ILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESILVQIK
                     KLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYNGKIG
                     ESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            404..874
                     /locus_tag="IW746_002077"
     CDS             404..874
                     /locus_tag="IW746_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078969.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            888..1661
                     /locus_tag="IW746_002078"
     CDS             888..1661
                     /locus_tag="IW746_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1078970.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            1675..2487
                     /locus_tag="IW746_002079"
     CDS             1675..2487
                     /locus_tag="IW746_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1078971.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            2541..3506
                     /locus_tag="IW746_002080"
     CDS             2541..3506
                     /locus_tag="IW746_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1078972.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            3622..6345
                     /locus_tag="IW746_002081"
     CDS             3622..6345
                     /locus_tag="IW746_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1078973.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            6358..6990
                     /locus_tag="IW746_002082"
     CDS             6358..6990
                     /locus_tag="IW746_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1078974.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            7889..>8680
                     /locus_tag="IW746_002083"
     CDS             7889..>8680
                     /locus_tag="IW746_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1078975.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         2946 a         1307 c         1712 g         2715 t
ORIGIN      
        1 taattcttgc cagccatggg gaatttgctc aaggattaaa gcaaacagcg acaatgatta
       61 ttggagatag tgtaccaatt catgccttgt cagcatttag agatgaagat gaatctattt
      121 tggtacaaat aaaaaaactt ttagccacaa tagatataga agaaacctac attttaactg
      181 atattctagg tggtagtgtg aataatgata tgctcactat tattaaagag tatgagggat
      241 tgcaattaat tactggaatg aacttacctt tagttatttc tattgcaacc tataatggaa
      301 aaattgggga aagtgagcta gaaaagctta ttaaagagag tcaacaatca gtgattgatt
      361 gtagaaaatt attagtagaa caacttactg gaggagatga cctatgatta agttagtacg
      421 tattgaccat cgtttgttgc acggacaggt agtattttct tggagtaaat cattaaatat
      481 caatcgtatt attattgcca ataaagaggc agcgaatgat gaattcaaaa aaatgtcttt
      541 gaatttatct aaaccgactg gcattaaatt aacaattttt tcagttgaac agttactaga
      601 aaaaatgcct aaaatcgaaa cacttaacga aaatatcatg cttatttttg gtaatacaaa
      661 agaaattcgt caattttgtg aagcctatcc taaagtacaa gaaatcaact atggagggat
      721 aatcaaaaaa gaaggttcca agcaatttag taatgccatt ttcttgaatg aatctgagca
      781 agaagatgcc aaaaaattaa aagctcttgg cattaaacaa tttattcaac aggtaccaac
      841 ttctaaaaaa gaagatttga atacaatgtt ataaaggagg aatgaaaatg ctcgttcagt
      901 caattggttt agggttaatc ggtgtttttt gtattttaga ctcaaggctt ttaggaagaa
      961 tgaattttga acgaccgttg attgtcagta cgcttgttgg tttggtactt ggagacatgg
     1021 aaaaaggatt aattgttgga gcctctttgg aattgatgtc cttaggatta gtaaatatcg
     1081 gagctgctgc accacctgat atgaatatgg catcaattat tgctacagcc tttgccattt
     1141 tatcaaatgc gaatgcagaa gcagcattaa cgattgccat tccaatttca gtgttaggtc
     1201 aaatactagg tatcttaatg cggacgattt tgtcaaattt gactcacgtt gcggatcgct
     1261 ttatcgaaca agggaaattt agccaagcaa ggagaattca tatactttat ggctcgggat
     1321 tatatgctct catgtatttt attcctattt ttctagcaat ttattttgga acgaatcttg
     1381 tttctcagat tgttgatgcg attcctgagt ggctaacaaa tggcttaacg gttgcaagta
     1441 aaattttacc agcatatggg tttgcgttgt tattacaaac gatgttaacg aaaaaaatgg
     1501 tgccattttt attacttgga tttttaatta ctgcttattc tggattggga atcattggta
     1561 ttgcagcctt cgcatgtatt gttgcgtttg tcatggttca agttcaaggt aaaaactcaa
     1621 atggtaacca aaatgagcca gatccattag atgacttata aggaggtaga ttaagtgaaa
     1681 gagaaagatc aaaatgcatg gaaatatcgg cagttcttat ggcgttcatg ggcaattcaa
     1741 gcctcatgga actatgaacg acaaatgaac atgggtttta tgtacggaat tgcaccaatt
     1801 ctagataaca tttacaagaa tccagaagat gaagcaatga agaaagaagc ttacgagcgg
     1861 catatgatgt actacaactg tacccctcaa acaagtgcat ttgtgttagg gttaactgct
     1921 tctatggagg agcaatacta cgaagaccaa gaaaacttta atccggaatc aatcagtgca
     1981 gtgaagactt ctctgatggg accgctatct ggtgttggtg attctttctt tcaaggaacg
     2041 attcgggtat tagcctttgg actaggaatt aatttagccc aacaaggtag tattttaggt
     2101 ccaattctag caatgttaat ttcctttgta ccttcctttt tagttactta ctatggtggg
     2161 aaaattggct ataacatggg gaataaatac ttgtctaaac tgtatcaaga aggtttgatg
     2221 gataaagtaa tgtatgtttg ctccattgtg gggttgatgg tcattggttc gatgattgcc
     2281 agcatgatcg ggattacaac accaattcaa atgggtaaag cttttgtctt acaagatgta
     2341 ttagatagta ttatgccaca atttttacca ttaggattaa ccttcttcat gtactggtta
     2401 ttgcagaaaa aggtcaaaac aggatggatg ttggcaatct gtttagtcgg aggaattatt
     2461 tttagtttgt taggtatctt acaataaggg aataaccaat aaatttggtt gaataatata
     2521 aataaggagg aattcaattg atgaattcaa aagaaattgt taaagaaatc gtagcgaaaa
     2581 aagaggtaaa atcaatcgta tttgttggct gtggcgcttc aaaagcggac ctttatcctg
     2641 caaagtattt tctagaccaa aatgcgaagc agttacggat tagtcattac acagccagcg
     2701 aatttaacta tgcaacacca gcatctgtag atgagtcaac tgtcgttatt tcagcttctt
     2761 taggaggtac gactccagaa acagttgaag ctaacgcagt agctaaagaa aaaggtgcta
     2821 cagttattag tctaacacga agcattgact caccgttaac aaaagatgca gattatgtaa
     2881 tttatcatcg atttgcagag aactatgctg ctaaattaga aaaaacaggc tactgtttag
     2941 aattggcagt agaactttta catcaagtag aaggttatga ccattatgaa gcaatgcaag
     3001 aaggatttgc tactatctat gatcttgccc aaaaaagtgc agaatcagct cgtaaagatg
     3061 cgaagaaatt tgctgaacgc tttaaagatg aaaaagttat ttatgtaatg agtagtggtg
     3121 ctacacatga agtagcatac tcaacatcaa tttgcttgat gatggaaatg caatggatta
     3181 attctggtac cttccattca ggagaatttt tccacggacc attcgaaatt gtggataaag
     3241 acgtaccgtt tatattatta atgaatgatg gaaaaactag aaatatagat gctcgagcat
     3301 taactttcct tgaacgcttt gatacgaaaa cagaagttgt agatgccttg gattatggct
     3361 tgtcttcaca tattgctaaa gaagtcttag actactttaa cccattcgtg attactgcag
     3421 tatttagagt ttatgcagaa gaattgtcag aagcaagaga acatccatta actaagagac
     3481 gttatatgtg gaaactaact tattaaaaaa tagatcaaac taaataaggt ggcttgagaa
     3541 attaagccac cttgttttct tcaatgtgat tttatacata tggtaaaata actagtacat
     3601 attgtgaaag caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg
     3661 cttttgaaat ctacgccttc agataattat tcagctcgca gtatttcgca agctttacag
     3721 ttgaatagaa gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata
     3781 aaagtaaaaa gttacccggt tttattttta catagaacag ctcttgaaga actacatatc
     3841 acactgaata actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc
     3901 gcattagatc aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa
     3961 acggcagtct tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca
     4021 gggaaaacct ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac
     4081 acatcggcac ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta
     4141 tcctctgctt tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact
     4201 ggtttgctag aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct
     4261 gaagaagggc aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg
     4321 gataatagta ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata
     4381 tatacgactt tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga
     4441 tttcaacaac gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag
     4501 agtaaaattt tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat
     4561 agtgattttg aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac
     4621 gcatatatcc accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta
     4681 gagtacagtt ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga
     4741 atctactatc cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt
     4801 caattttttg accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt
     4861 aaactatttt tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt
     4921 caagaggatt acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa
     4981 agtattgatt ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct
     5041 ttggccaact atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa
     5101 aagaaaacca aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg
     5161 tatgcaaaaa aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag
     5221 gacattttct ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt
     5281 attaagagta ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc
     5341 aactgtatgc tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta
     5401 gagaaaattg aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg
     5461 attttattgg tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta
     5521 aatggaccac tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa
     5581 caagtattaa acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact
     5641 ttcaaaaagc agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt
     5701 tatacaggga ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat
     5761 gcagttagag aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat
     5821 tttgagactc cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa
     5881 attaatcaga ttccatatat tggattagat cgcctgatta atggggaagc aattggcgaa
     5941 tttgttcatt acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta
     6001 attttcaatt tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag
     6061 gtgctatctt ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt
     6121 tctaataatc agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta
     6181 cgtaaagaaa gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa
     6241 ataatggata aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata
     6301 ccagatttag agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg
     6361 acaatgggag taatttttga tatggatggg gtactcatag acagcgagac attttatttt
     6421 gagagaagaa tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat
     6481 gattatgtgg ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca
     6541 ttaagagagg aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc
     6601 gctaaagatt tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata
     6661 ccattggcac tggcatcttc ttctccagtt gttgagatag agagtatgct cattcaatgt
     6721 gaacttactg aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca
     6781 catcccgaaa tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta
     6841 gaggattcaa cgattggtat taaatcagca aaggcagcaa atatctatac ggtggcacta
     6901 aaacaaccaa tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa
     6961 ctggaacaaa ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg
     7021 tgctagctat tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct
     7081 ggtaacacct tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt
     7141 tattttgttc gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg
     7201 caacattcta tctatttcag aacgtaacct tccccagttc attgggattc attagcatac
     7261 aaccaacatt tcaaaaagaa attgcactac accattatta gctattttga ctaaccccaa
     7321 aaaacttgct ataattcagg tgtaggtaaa aggtgctggt atcacctttt acgaaactgc
     7381 ttcggcagtg gtaatgagtg tggcttaaag gactagtcct ttaagctttt tactttgttc
     7441 gcaatctcta agatcaagtt aatgatcgat aagattgcta gaatattttg caacattcta
     7501 tttcagaacg taacctttcc cattccactg tgattcattg gcaaccctcc tcacaaccaa
     7561 cgtttcaaaa gaaattgtaa agttgttggg gtaccacgac cgaacaatac tacacctgaa
     7621 tgatagtaag ttgtctcatt aagtatagca tatcctcttt ttgggtaaat agaaatcggt
     7681 tacatttttt atccaagacc aagttttttc atttggtctt atttgctata cccaaacaat
     7741 tccgcagtat agtcgacggt attgatttta tcaggtgagt aatagaaact tgttttcctt
     7801 ccccaaaaca ttacctataa aggagaaaaa atggtatcgt aattatatag gtataggatg
     7861 aaattttttt ctaagaggag ggttgttgat gaaaaattta cagttaaaat ttattgttga
     7921 tccatcgatt gtacgctggt ttcggattat caatcaattt gagcgcaatc aacttgccac
     7981 aaaaggagaa ttagcaaaga gtaatcatgt atctgaacga accattcaaa cagatgtcaa
     8041 taaaatgaag gactactttt ctgaaagtgt tcaatttgtt tcaacgaaaa gtgggtattc
     8101 ttttcagaag gaaagaccgg ttctttttct caagcagaag gagcaactac tggagaacga
     8161 aattttattt gagttgttag ggaatgtctt ttatggcgaa ttggaagata tgacggagct
     8221 aattgatcgt tttcatttat ctgaagcgac ttttcgtcgg tatttgaagc agattcaacc
     8281 agttttggaa gaatacggct tagaattgag tctttatcct cttgatttaa aaggagaaga
     8341 ggcaaatatt cgcaagtttt ttaaagactt ctattatgaa ggggagatga cgccccatac
     8401 tcttgttcca ccaagggatc ttcatgaact tgtgcaagct acattctatg ataaatttga
     8461 gtatttctcg attggaacag ggacttctcc tgctgaattt tattatagtt tgtatattgc
     8521 gattgagcgt gtgagacaag gaaaaacgat agccattcct caaggtctga ttgagcgagt
     8581 aattgctagt gagaattttc gcttaatgta ttccttgaaa gaagcgattc aaacaaatta
     8641 tcaagttgct ttatcagaag aggagttttg ttggttgtat
//
LOCUS       DACEND010000105         8614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEND010000105 DACEND010000000
VERSION     DACEND010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.108"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            276..458
                     /locus_tag="IW746_002084"
     CDS             276..458
                     /locus_tag="IW746_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078976.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            442..627
                     /locus_tag="IW746_002085"
     CDS             442..627
                     /locus_tag="IW746_002085"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1078977.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            870..2390
                     /locus_tag="IW746_002086"
     CDS             870..2390
                     /locus_tag="IW746_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1078978.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2577..3149)
                     /locus_tag="IW746_002087"
     CDS             complement(2577..3149)
                     /locus_tag="IW746_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1078979.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW746_002088"
     CDS             complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW746_002088"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1078980.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3834..4349)
                     /locus_tag="IW746_002089"
     CDS             complement(3834..4349)
                     /locus_tag="IW746_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1078981.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4351..4635)
                     /locus_tag="IW746_002090"
     CDS             complement(4351..4635)
                     /locus_tag="IW746_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1078982.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4669..5997)
                     /locus_tag="IW746_002091"
     CDS             complement(4669..5997)
                     /locus_tag="IW746_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1078983.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6011..6466)
                     /locus_tag="IW746_002092"
     CDS             complement(6011..6466)
                     /locus_tag="IW746_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1078984.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6469..8415)
                     /locus_tag="IW746_002093"
     CDS             complement(6469..8415)
                     /locus_tag="IW746_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1078985.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2861 a         1609 c         1359 g         2785 t
ORIGIN      
        1 aaagattcga ttaaattagt tgagtaaatg cttcgtacga acatagttga tttattcacc
       61 atacttccca ttacatcgta aaacgctata tttaagattt caaacaacta tattaatagg
      121 aagaaaactt catttttcct cttagttcta taaaaagtgt ggctaatttg aggcaaaata
      181 ttaaattaaa tgtaagcgtg tcataggata gtatctcgta cgccgattat ctttcttcta
      241 taattaaact atcaaatata gaaggagaaa aacgaatgaa taaaaaagat attgtaccag
      301 ttcaattttc aagtgcggct caaaaatcgc aaattgacac caagccaaaa ctagcttgca
      361 gaatcaaatc ggaagataag gaaatattta tttataatgg aatggataaa tatcttttgt
      421 ataccttgtt aaaagagttg aatgccaatg ctcattgatg caactgcatt gaaacatatt
      481 tttatcgttt gtggaaaaac agatcttcga aaaggaattg atggactagc agctttcgtt
      541 atagaagaat atgaccttga tgtttacgat caagcgcttt tcttattttg cggtacgaag
      601 aaagatcgct tcaaggctct ttactaggat caaaatggat ttgtattaat gtacaaaaga
      661 tttgaaagtg ggtaccttca atggccgcgt tccagaaaag aagtcttact gactggcctt
      721 tcaatcgaag aaaaatgtac gctaaaagaa acgaaaaaag gtgcgatata ttagacgaaa
      781 accttgttaa atcaaggatt ttgtctgttt ttcgttgtca taaaatggta taatagatgt
      841 aagaaaatcg ctagaatggt ggtgtaaaaa tgacaaaaat ggaatctgaa cttcttgtac
      901 aaatcaagca attaacacag ttgcttgaaa tccagcaaca ggaaaatact cttcttagag
      961 aacaaattgc tgaaatgaat cgacgcttgt ttggacgaaa aaaagaaaca ccacccgtag
     1021 atgggcaaat cgatttatta gacgactcaa cttttaatga accagagcac actggacaag
     1081 aaagccagga acccattacg gtatcttctt ttaaacgcag aaaacgcaag ggactcaaag
     1141 cactttcatt agaaggtctg cctgaagttc ctattcatta tgaattaaaa ggtgccgatt
     1201 gtttgtgtga agactgtggt catactttac atgatatcgg tgcgacaagg cttagagaag
     1261 agccattgta tgttcctgcc catatagaaa aacaagtcat ctaccaacat gcctatcatt
     1321 gcagagaatg tgaagcatta ggagaaacaa aaatcaaaaa agcgcctgtt cctaaaccgt
     1381 tgatcaataa tagtctaggt tcctcttcaa ttgtgacaca gagtatttgg gaaaaatttg
     1441 ttaaaaaggt gccggcatat cgacaagaaa aagaatggca tgcacttggt ttccctcttg
     1501 atcgtaaaaa aatcactaat tggcatatca aggtaagtga aatggtttta gagccaatct
     1561 acaattgcct tcatcaagaa ttaagcaatc aggaggtctt acacgcggat gagacaagct
     1621 atcgggtatt gaatactcat aaagaaaaga ccttttactg gttatatgct tcaggtaaaa
     1681 gcgaagaaaa acaagttgtg ctctacgagc acgcaaattc gcgttccgtt gatgagccaa
     1741 aaagtttttt agaatcattt agcggtttct tgcatactga tggttacgcg gcctatggaa
     1801 agctgccggt aaacaatgtt tattgttggg cgcatgttcg cagaaagttt tttgaagcat
     1861 tgggtaaaca accgtcccca aaaagtcatg ccaaaatagg attagacttc tgtgacaaga
     1921 tgtttcgaat cgaacggacc tttaataagc ttagcgcaga agaaaggttt gtagcgagga
     1981 aggaaacgtt gcagcctgtg atgactgaat tttttcattg gtgtgagagt tttgcttatt
     2041 tgccaaaatc gcagcttgga aaggcggttc aatacgcagt caatcataaa gacggacttc
     2101 agattgtgtt gcttgatgga cgcttggaac tttcaaataa ccgagcagag cgtgccatca
     2161 aagagttagt gatcggtcga aaaaactggc tattttctaa aagcttaaaa ggcgctagat
     2221 caaatggaat tatattgagt attatccaga cagctgttgc gaatgggtta aacatccgca
     2281 aatatttaaa tcatcttttt acagagattc ccaatctgtc ttcgatgaca cctgaagcgc
     2341 tacgcgctta tttgccatgg aatcagcaga tacaagaaat ctgtaaataa tataagccac
     2401 aaataacctg atttgagtgt atcaaatcgg gctatttgtg tttaggtacc attctatgac
     2461 acgcttacgc tcaaaactac tgaattatta agttatgtct aatcaaataa aaaaaccatt
     2521 ctcatatttg ctgaataatt agcatttaga gaatggtttt ttatctttaa aatctactac
     2581 tcaaaatctg catgtcgagc aaacatcgtt tctgagcttt cacctgtttt atccattaaa
     2641 gataacacta aactatcaaa ggtgagaaat gctaattgtt caaaacttga acccataggt
     2701 tgtgaaaaag tatcttcttc tcgttgattt tcatctttgg taatacctgg taatacaaga
     2761 acagaatctg ctaattgacc aatcgacgag tcttttttca tggtaactaa agcaatgctt
     2821 acaccacttt gatttgcttt ggccgctaaa ttttttaaac tacctgtttc acctgagcca
     2881 gaacagataa ttaataaatc tccaggttgc gaatgtggtg aactaatttc accgacaagg
     2941 ctgactgaaa aacctaaatg cattaagcga ttcgtaaatg cttgcatggc aattccagag
     3001 cgtcctgcac cagctagaaa gatgtgttta gcttgaacaa ttttctcact aaattgttct
     3061 aactctacag aattaatacg ttttgagtta ttttgtaatt ctaagataat cgcttctaaa
     3121 tagtttttat tttggtcttt attggtcatg atcttttact tcacttcctc taatgattga
     3181 tttagtttat caatttgttc ttgcggggta tttttcccat tgaagatgaa accgcctgat
     3241 acaaaaatat ccgcccctgc tttagaacaa actttaatcg tttgattatc aatcttgcca
     3301 tctacttcaa tatcatacgt ataatcattt tgtttgcgta ttacatctaa ctgttcgatt
     3361 ttaactactg tctcttgaat aaaatgctta ttctctgttc ctggattgat tgtcattact
     3421 aatacttgat cgactaaaga taatactggt tcaataaaac tgactggtgt tgcaggatta
     3481 aaagtaatcc ctacttttgc tcctgcttcc ttgactaact gaatgacctt cattgcgtgt
     3541 ttggtagctt caatgtggaa ggtaacaata tctgctcctg cttctacaaa tcttgtgaca
     3601 attttttctg gtgtatcaat catcaaatga acatcaaaca caacgtctgt tagtggacga
     3661 atatctttaa taacacttgg tccaaaagcc atatctggta caaaacagcc atccattaca
     3721 tcaatatgca aataatcaac attcttttct tttaagattt caaataactc tttaacttct
     3781 gcgataggtg catttaacaa tgatggagca attgtcattc tctattcccc cgtttattct
     3841 cccataatca tgagattgat tgttctagtt cgctctctta gttgatccca atcgacaaaa
     3901 tagacacggc ccatttctcc taataaaagt ttaccttctt gaacaattaa agtcattgag
     3961 ttggttccat aaatagatga acgtaaatgt gcatcggtat ttagcatgac ccatttttct
     4021 gcagggtaat tgggatcaga taatcttgtt ccaaaatcaa tgtgttttgg ccccggactg
     4081 ttgtattgat tttcactcgt catttttgga ataatcgttt ccattacatt gttgatgtct
     4141 acatgtaaat aatcatcacc aaaaaagttt gtgtcatgca tcgtttcttc aaaaaagagt
     4201 gagcaagttg tatgcgggct cgaaattact aacattccat tctcaatacc tgctgtatca
     4261 actgtatcta aaagttcttt tcgaatatca tgatacgaag gacgccctga aactgtttca
     4321 atagtcaaag tgtcatgatg aaaacgcata ttaaacacct aatctttctt ttacatctgc
     4381 ataaatggca tctgtaccaa tacctgttaa taagcccaag ccattcgtta ctttagcttt
     4441 aattacatca gggacaattg ttgtacttac tacagcatca tattcatcaa ttttatttag
     4501 ctcatcagca attttagatt gataaatttt cacttgatct gttaatcctt cttcttccaa
     4561 ccattttgat aattttgcta ctgcaattgt agaagtagca tgacctgctc cacaaataac
     4621 taataatttt ttcatattga tctcttcttt cttattttaa ttttatgatt aagctttctt
     4681 cactttgttc atgtagatag aagcacctac taaaacaact cctaatccac ctattccaat
     4741 tacaccaaag taagatgcta ataaaacaaa caaccctact ggccataatc cacataacaa
     4801 catagttact aatcctgctg tccccacatc gaaacttgct aatgtaaaca catcagtgat
     4861 aattggtgcc aaccatgttg ccatatataa tgttgtaaca acatacaacg aacatcctac
     4921 taatgatctc acaatatttc ctttaaaata aggaatcatt aaacagatgg cgaaagcaaa
     4981 gaatgctaaa tctccaaaag gtaatacttt atttcctgga agaattagtg ctagcaataa
     5041 tgagattggt acaagcaata gagaagttga cataatagtt ggatgaccca cagttaacgc
     5101 tgcatccatt ccaatgttta cttggcgacc atttaacttt ttattcgtaa attctttcgc
     5161 cgcttctgca attggcatta aaccttccat aaacattgct accattttag gcataatctt
     5221 catcactgcc gcagtcataa ttcctaagtt tccaattcct gctacgtcat agcctgccat
     5281 aatacccact acaataccga taattaggcc aataacaact gtgtcaccta aaacaccgaa
     5341 acgttttgtt aatgtttctg catctgcatc tattttatta aatccaggaa tacgatcaaa
     5401 aacccaattt actggtttgg caaatagtac tcctgataat cccatcatat gtgtgataga
     5461 gattcctgga tagccgaaaa attcgctaac agttttttgt gtacgatctg ctaagaacca
     5521 ttgtaaaatt actgcgacaa tcgcgccaat taagccaaac caaaagtttc ccgtaacaga
     5581 ttgagtaatc atacctagga aaattgcgaa ccaaaagttc caaatatcaa cattcaatgt
     5641 ttttgtccaa cccaaaacca caaatatgaa gttaagaatt aatgaaattg gaatcattaa
     5701 aattcccaat gatgtagagt aagccaaagg tccaccaaca cctacgccaa tatctaggat
     5761 attcaggcca ccaccgtatt gttcccccat ttgttgaatt gctgttccta aaccatctga
     5821 taataaactt aaaactaatc ctaaaccaat agaaccgata ccaacagtaa cgccggaaac
     5881 aaatgcttta ccaggtttta atccaaataa tatcccgatt acaaaaataa tcagtggtaa
     5941 aaagacgttt gaccctaatc caataaacca ttcaaaggca tttaatacaa tatccatctc
     6001 tctattcctc ctaataaact cctgcagaat gtactaactc aataaatgct tctttcgtgg
     6061 tacatgcaag taattcattt actttttctt cattttgaaa taggctaact aagttttgta
     6121 gtgtatcaat ttgttcatga ggttgtgaca ttgccaacat aaagactaaa cgtacaggta
     6181 tttcatgaga tttatctgtc atatctgaaa ataccagtgg ttcctttaat gaggcaaaac
     6241 agatttgaga gctatttaca tgtgcggaat ctgtatgtgg gatagcaaac ccaatttgat
     6301 ttacttctaa tccagttgga aattcttgtt cacggttggt gataccttct aagaaactat
     6361 ctttcacgca accagcgttt tttaattgtt cagacattac ttgaaacaat tctttttgat
     6421 ctttcacttc taattgaaat aaacataatt ctttatctag atacattttt attctccttt
     6481 ttgttgaaaa attcttagta atttttcgct atcatgaact tgtaacaagc gatcgaccat
     6541 ttctttttgc ccaataaatt ggtaaagttc ttcatatact cctctaatct cagatacatc
     6601 ttcttcagct aaactaacca taatgactaa tttgactttt cgatcgcccc aattaatcgc
     6661 attttttaat gctacaataa aaatgttgga ttcaatgacc attgatggat tcgcatgcgg
     6721 taaagcaaca ccactttcta aattagtatt ccccattttc tctctagaga agatagaagc
     6781 acgaaactct tcagtaacta atgaattggc ttctagttcg gaaatcatca aatccaaaca
     6841 ttcttctttg ctttccacct gtactcctgt ttttactaaa gatgattgaa caaatttaga
     6901 tatggtggga actttaaccg ctttaggaat cagttcattt tgaataaatc gcgaaggttc
     6961 ttctattaaa tatttagtat aagcttccat aatattcata taatctgttt tacttaccaa
     7021 tggagatacc cttactaagg gtataccacc tgcatcaaag gaaagagagg aaataatcaa
     7081 atcaacttcc ccaatttcag ctaaatgaaa cttatctaaa gttgatattt ctacattatc
     7141 ttttgctggt aaaaaccgtc gaactttatt gtaaattaat tgcgcagaag aaataccgta
     7201 ttggcaaata atgatgatat tatttgcttt ggactgatct tcaatagcaa tctgaaaatg
     7261 aatcaaaatt aaggaaactt catcgtccgt taaagtcaca tcaaattctt cttcaaggat
     7321 ggacatggcg taccatacta tgctaaagag ttctgaatat tgagatttga tattttctaa
     7381 taacggattt tgaattttaa tacctttttt taaacgaagg accattgctg gcacatgata
     7441 caataaggat tgatataatc gatcattttg agtcaaatca attttttcaa tctcactcat
     7501 tctttcaatc aatttatgaa cgctcatagc gtactcttca ttgctggttt ttaaattatt
     7561 tgtcacacga tgtgcaaata gttgacgaca tagatattct ttatctgcgt cataaaatgg
     7621 aagattcaat tttgccttca attcttcaat taaaccattt gccacaatgt acgtttccat
     7681 atagcgaatg ctattgaaga ggaattcttc ttcaacagtt aaatgatgac ctttcagtaa
     7741 acggttacat tggataacta aagtagaaat caatgaccga acatagtaat cagaaacctt
     7801 ttcagtcagt tcactgtaat cattgaatag caaatcatga atgacactaa taacttttga
     7861 atcaaacaac atatttaaaa cttgaaaaaa tgtttttgat tccatgtcat tcgcataata
     7921 gaatacaagt tgcttgattg ctcgttgaat tgcttgttca ttaccagtaa aactaattcc
     7981 ttctgcagat gactgcaact taacttctcc actgccaata attttattga taattctcag
     8041 gtcattgtat aacgatgttt tactaaccat aaaactttga gacaaatgag ttaaagaaac
     8101 aggcttatca ccaaacaaaa cttctttaat gatctcgaca cgtctttttt ctggagaata
     8161 aatatctttt tcaagataat tttctaccga tgaaatcaaa tgttcaacgt ttttcctatc
     8221 acctatgagt ttgataccca aagaaggtaa tcgctcaatt gttacttggt aattaactac
     8281 ataatcttcc aacaattcaa tatcttgata aatggtcttg gtcgacactg ccaatttatc
     8341 actaaaaaaa gaagcgggtt taaagtcttc ttgtcgactt aataatttta ataaatttac
     8401 atgtctttca atcaaacttg ttcacctctt aacgctagca tttgttacct cttttcatta
     8461 tttattctag cactggaacc cccttccctc aattacatct atgtcaataa tacttgtgga
     8521 agtttgttgt aaaggaaatc caaataatct taaaaaatga gaaacaaaaa aggagaaatc
     8581 cttaaaagag ctaagatatt ccaatagaaa atac
//
LOCUS       DACEND010000106         8438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEND010000106 DACEND010000000
VERSION     DACEND010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.109"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            754..1038
                     /locus_tag="IW746_002094"
     CDS             754..1038
                     /locus_tag="IW746_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1078986.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            1041..1958
                     /locus_tag="IW746_002095"
     CDS             1041..1958
                     /locus_tag="IW746_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1078987.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1951..2733
                     /locus_tag="IW746_002096"
     CDS             1951..2733
                     /locus_tag="IW746_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1078988.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2734..3414
                     /locus_tag="IW746_002097"
     CDS             2734..3414
                     /locus_tag="IW746_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1078989.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            3407..4303
                     /locus_tag="IW746_002098"
     CDS             3407..4303
                     /locus_tag="IW746_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1078990.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            4498..6147
                     /locus_tag="IW746_002099"
     CDS             4498..6147
                     /locus_tag="IW746_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1078991.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6741..7676
                     /locus_tag="IW746_002100"
     CDS             6741..7676
                     /locus_tag="IW746_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1078992.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7669..>8438
                     /locus_tag="IW746_002101"
     CDS             7669..>8438
                     /locus_tag="IW746_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1078993.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2629 a         1452 c         1832 g         2525 t
ORIGIN      
        1 tagaaatttt gatagggcaa aaaaataata acacgaaaat acaagtttct tagaaatcat
       61 tttttattga gatatctaga taaatgattt attcttcaat caaccaactc gccaagctga
      121 ggtaatgttt tgaggtaatg ataacataca actttttata ccctagtaaa actctataaa
      181 caaagactta ttaatttcgc atgaaataca ttcctgtaaa atgcaataaa atagagtttt
      241 gtttaaccag gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga
      301 ttatcgttcg agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt
      361 taccttaatc ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt
      421 ttagctatgt taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg
      481 ggaaaaagat aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa
      541 gtggtcagct tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat
      601 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg
      661 tgaaaagtct tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg
      721 tattattttt cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc
      781 tggcttcagc gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg
      841 attaccatgg tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag
      901 acaactcaac cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta
      961 cactttcttt atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc
     1021 aataagaaag aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta
     1081 taaccaaaaa atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg
     1141 gaatgatcta tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta
     1201 ttatgggttt gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc
     1261 aggcagcaag acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa
     1321 ctgcaagaga taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg
     1381 aagacctgct gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct
     1441 ctttaggtat gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt
     1501 tgattttgga tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa
     1561 tcatccaacg tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag
     1621 atgaattgtc acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag
     1681 aattatcgaa agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg
     1741 cgacggaaca agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat
     1801 ctagccgtgt tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat
     1861 tggtagaggc aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt
     1921 atttcttaca attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata
     1981 aaagtatgga agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt
     2041 ttttattctt ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca
     2101 acggatacag tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg
     2161 attcttctgg ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt
     2221 acgattcgca atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt
     2281 ctcgttacgc ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt
     2341 gcttacacac ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat
     2401 gttctaaaag tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg
     2461 gctttgacat tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc
     2521 atgggaacgg ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg
     2581 gagtggttta cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg
     2641 caagcaccga aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga
     2701 atgtttattt tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt
     2761 atcgaagacg atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa
     2821 cttcttgatg cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat
     2881 ttgatccttc tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc
     2941 cgacagacat cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa
     3001 gtttccttgt tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa
     3061 ctgttgttac gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg
     3121 tcgcaacgca tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg
     3181 aatggtgtgg ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa
     3241 catccgaaaa aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat
     3301 tttgatagtg agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag
     3361 gcacatgctg tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct
     3421 gttgcttctc gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa
     3481 gagattaact aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc
     3541 agtcgaattt cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga
     3601 tgaaaatgaa caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat
     3661 cgtcggcctt tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt
     3721 actggaacag gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc
     3781 tgttgcaaga ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa
     3841 gcaaaaagaa atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt
     3901 ctctttttat gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat
     3961 aaatacaact gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca
     4021 gaatttattg cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt
     4081 cattcgatta acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt
     4141 cttccagcgt ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg
     4201 gctttacctg tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga
     4261 cgggggcatc ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct
     4321 gaagtgaatg acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt
     4381 tttattttgg tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt
     4441 ttcaaatagc gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg
     4501 ggccaaagag aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta
     4561 gacatttttt cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct
     4621 tatcaaccga gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa
     4681 ttccgttcat gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa
     4741 gtatttgaag ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt
     4801 ttacgtagct ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag
     4861 gaaaatcctc aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat
     4921 cataattttg caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt
     4981 ttatttattt ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa
     5041 gtactaggaa tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg
     5101 aagaaacaaa cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca
     5161 gccgccaatc agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact
     5221 aaaccattaa gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa
     5281 gcagaattga tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat
     5341 ttcgttctca ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg
     5401 ggagaattgg aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc
     5461 cgaccagagt ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa
     5521 gaagcggtag ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca
     5581 tcaggaaaat ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc
     5641 caaacagcga ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca
     5701 atcgaagatg atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa
     5761 cgtttgctgg accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta
     5821 aaaggaacaa atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg
     5881 gagtatccat ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag
     5941 aattattgtg agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt
     6001 gatttcaaat taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt
     6061 ttgaattatc ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa
     6121 cagcgtcagt ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa
     6181 aaagacgatt atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac
     6241 gagtctgctg tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg
     6301 ctgtcttcct tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt
     6361 ccatggcaaa gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc
     6421 attttattta tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag
     6481 cttaacatga ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta
     6541 gtagttgctg tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc
     6601 tatcgaagat gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg
     6661 tgtggaagcc aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc
     6721 tataaggaga taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct
     6781 atggaagtgg ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct
     6841 atgcgctatt agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt
     6901 tagtcaataa aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag
     6961 ttcgagcaaa gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa
     7021 cggtccagca aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga
     7081 tgaaacgcag cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg
     7141 cacgtatgct ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg
     7201 aaccacgttt gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg
     7261 agatgtggga gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc
     7321 attatctaga agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac
     7381 tgatcgagaa tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct
     7441 ttgatttagc gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag
     7501 atgagcagac aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc
     7561 agcttacaag acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag
     7621 aactgttctt gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt
     7681 tattttactg cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg
     7741 gtccaaacac tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa
     7801 atgatcggtg ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct
     7861 ggtttgatta tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc
     7921 tctcaaaaat ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg
     7981 attatttggg gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta
     8041 acgatcattt ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt
     8101 acattattga tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct
     8161 cagtcttatg atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta
     8221 ggtggcgttt tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc
     8281 aatccgatcg tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg
     8341 ccagtagcaa catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact
     8401 tggtatttga tcaataaagg aagaggattg agaagtta
//
LOCUS       DACEND010000107         8311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEND010000107 DACEND010000000
VERSION     DACEND010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.110"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(10..687)
                     /gene="nth"
                     /locus_tag="IW746_002102"
     CDS             complement(10..687)
                     /gene="nth"
                     /locus_tag="IW746_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1078994.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(694..1392)
                     /locus_tag="IW746_002103"
     CDS             complement(694..1392)
                     /locus_tag="IW746_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1078995.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1660..2844
                     /locus_tag="IW746_002104"
     CDS             1660..2844
                     /locus_tag="IW746_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1078996.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2850..3293)
                     /locus_tag="IW746_002105"
     CDS             complement(2850..3293)
                     /locus_tag="IW746_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1078997.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3519..3806)
                     /locus_tag="IW746_002106"
     CDS             complement(3519..3806)
                     /locus_tag="IW746_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1078998.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3977..5329)
                     /locus_tag="IW746_002107"
     CDS             complement(3977..5329)
                     /locus_tag="IW746_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1078999.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5602..6048)
                     /locus_tag="IW746_002108"
     CDS             complement(5602..6048)
                     /locus_tag="IW746_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1079000.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6284..7210)
                     /gene="cysK"
                     /locus_tag="IW746_002109"
     CDS             complement(6284..7210)
                     /gene="cysK"
                     /locus_tag="IW746_002109"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1079001.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7350..8237)
                     /locus_tag="IW746_002110"
     CDS             complement(7350..8237)
                     /locus_tag="IW746_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1079002.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2241 a         1718 c         1394 g         2958 t
ORIGIN      
        1 aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt
       61 tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt
      121 gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg
      181 tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct
      241 caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc
      301 atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc
      361 tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat
      421 gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat
      481 tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt
      541 attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg
      601 attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc
      661 ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga
      721 gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg
      781 ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca
      841 cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg
      901 cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg
      961 ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga
     1021 ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc
     1081 acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat
     1141 cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg
     1201 atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc
     1261 aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct
     1321 atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc
     1381 taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag
     1441 caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac
     1501 ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta
     1561 agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt
     1621 tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa
     1681 cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc
     1741 agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc
     1801 tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc
     1861 ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca
     1921 agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt
     1981 atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc
     2041 acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga
     2101 acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata
     2161 tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa
     2221 agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac
     2281 caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct
     2341 gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt
     2401 cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc
     2461 tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga
     2521 atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta
     2581 ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact
     2641 cctgtattca atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg
     2701 ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag
     2761 ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc
     2821 tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat
     2881 cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta
     2941 gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg
     3001 attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg
     3061 atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta
     3121 gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct
     3181 agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata
     3241 atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca
     3301 ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt
     3361 ttgtaggaaa acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc
     3421 cctttttctg attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt
     3481 catcacttaa ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct
     3541 agcacatatg gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct
     3601 gagtagatgc gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct
     3661 ggcatcgtgt aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac
     3721 tcatcaatat tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct
     3781 tgttggacaa aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa
     3841 agctgcttgt tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg
     3901 aagaaagaca actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg
     3961 tcacaaccct ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta
     4021 ggttcaagta attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga
     4081 gatcttctac agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa
     4141 tgtcgtattt ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta
     4201 gctccatcat tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata
     4261 cagtcgcttg gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac
     4321 cgatcgtcca gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt
     4381 tatgatctgt caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag
     4441 gaatatagtt ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa
     4501 aaatgtctgg attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg
     4561 tatctacttt tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca
     4621 tatctgtatc aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg
     4681 tgataaattc agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt
     4741 tttctaagat atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat
     4801 caatcaatgt cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat
     4861 atccaccacc aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg
     4921 cttgattgta gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga
     4981 taataggcca tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc
     5041 ctgttttcag atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt
     5101 gttccatttt aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc
     5161 ctgctgggtc ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa
     5221 tattgtcatt tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt
     5281 ttacagctgc agtacctgca tgagtacagc caattactac tactttcatt tatggtccct
     5341 ccgattaaca ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag
     5401 tttaatgctt gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga
     5461 acccaatatc tacaagattc ttattttaat aatattcact tttatatcta agattgtgaa
     5521 taaaagacaa aaaaataaaa aagctagcgt tttttttaca cagctagctt tttttaggta
     5581 taacacttgt agaaactgtt tttacatttg cgcctttttt gctgactctt tacaatccgg
     5641 acagagtcca tacaattcca accgatgttc attaatctca aaatcagtca gtctgcttgc
     5701 agccatttct atatcttcaa gccctgggta atgaaaatca acaatcttac cgcagttttc
     5761 acaaattaca tgataatgac gctttgaact gaaatcaaat cggctagaag catcgccgta
     5821 cttcatttct tgaacaaatc caatcgaagt aaataagcga aggttattgt aaacagtcgc
     5881 aacactcata tttggaaacc gatgttcaag tgctttataa atctcatcag ccgttggatg
     5941 ggaatgatgt tcaatcaaat attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt
     6001 ctcttttttt aacttttcga taacctcttc gactaactta ttttccatgt aaacacccca
     6061 cgttgttatt ctcgtcttat catactattt ttacatttta aaatccactt aacatgaaaa
     6121 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
     6181 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
     6241 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
     6301 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
     6361 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
     6421 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
     6481 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
     6541 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
     6601 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
     6661 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
     6721 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
     6781 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
     6841 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
     6901 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
     6961 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
     7021 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
     7081 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     7141 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     7201 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     7261 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     7321 atggtaattt ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa
     7381 gaataactgg acatagacat gccatttttc gccattagat catcaacaga catgccaaat
     7441 ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt
     7501 gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt
     7561 gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa
     7621 gtattaatca atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc
     7681 aagtacgctg ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga
     7741 taatcgcccg tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc
     7801 caataagaag aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat
     7861 tctttggttg ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta
     7921 taatttggcg cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg
     7981 atcatgactg aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt
     8041 tgatcgataa acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca
     8101 tgttgcggta ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga
     8161 gcaatcgctt ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg
     8221 gtcttcagct cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc
     8281 ttcttaaaac aaacttaatt aattaaatta t
//
LOCUS       DACEND010000108         8272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEND010000108 DACEND010000000
VERSION     DACEND010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.111"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(16..834)
                     /gene="purR"
                     /locus_tag="IW746_002111"
     CDS             complement(16..834)
                     /gene="purR"
                     /locus_tag="IW746_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1079003.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(1001..1825)
                     /locus_tag="IW746_002112"
     CDS             complement(1001..1825)
                     /locus_tag="IW746_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1079004.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1798..2478)
                     /locus_tag="IW746_002113"
     CDS             complement(1798..2478)
                     /locus_tag="IW746_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079005.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2501..3439)
                     /locus_tag="IW746_002114"
     CDS             complement(2501..3439)
                     /locus_tag="IW746_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1079006.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3684..4535)
                     /locus_tag="IW746_002115"
     CDS             complement(3684..4535)
                     /locus_tag="IW746_002115"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1079007.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4689..7262)
                     /gene="mprF"
                     /locus_tag="IW746_002116"
     CDS             complement(4689..7262)
                     /gene="mprF"
                     /locus_tag="IW746_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1079008.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7403..8209
                     /gene="proC"
                     /locus_tag="IW746_002117"
     CDS             7403..8209
                     /gene="proC"
                     /locus_tag="IW746_002117"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1079009.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         2519 a         1807 c         1418 g         2528 t
ORIGIN      
        1 ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt
       61 ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc
      121 gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat
      181 caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac
      241 acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga
      301 tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac
      361 aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt
      421 ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc
      481 aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg
      541 aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc
      601 ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa
      661 ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga
      721 agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg
      781 attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc
      841 caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga
      901 gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata
      961 aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga
     1021 aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg
     1081 tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc
     1141 ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca
     1201 taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga
     1261 tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg
     1321 gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga
     1381 ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc
     1441 atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc
     1501 cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg
     1561 agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata
     1621 caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc
     1681 ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa
     1741 acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca
     1801 tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct
     1861 gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg
     1921 gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc
     1981 tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact
     2041 gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat
     2101 ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa
     2161 ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc
     2221 tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg
     2281 atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt
     2341 caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc
     2401 tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga
     2461 tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta
     2521 aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat
     2581 tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg
     2641 tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct
     2701 tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc
     2761 ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct
     2821 gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg
     2881 cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat
     2941 atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga
     3001 gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt
     3061 cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct
     3121 gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat
     3181 acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat
     3241 gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt
     3301 ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg
     3361 cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg
     3421 tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt
     3481 tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt
     3541 ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc
     3601 tagccatttc actacaataa aaaactgaag aataccattt taggattcct cagtttttca
     3661 tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa
     3721 aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa
     3781 acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct
     3841 ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt
     3901 tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct
     3961 tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt
     4021 cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc
     4081 cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact
     4141 tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc
     4201 cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata
     4261 ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa
     4321 gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca
     4381 atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac
     4441 atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa
     4501 tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa
     4561 gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg
     4621 attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct
     4681 agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct
     4741 tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa
     4801 gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg
     4861 aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa
     4921 gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag
     4981 cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat
     5041 tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg
     5101 gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca
     5161 tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt
     5221 tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa
     5281 tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct
     5341 tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca
     5401 tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa
     5461 tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga
     5521 tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg
     5581 tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct
     5641 gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc
     5701 ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc
     5761 aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct
     5821 gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac
     5881 aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt
     5941 cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc
     6001 aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc
     6061 gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa
     6121 aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact
     6181 tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc
     6241 cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc
     6301 ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt
     6361 cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct
     6421 acatcttgcc gaacagacaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc
     6481 attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag
     6541 atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg
     6601 gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac
     6661 tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa
     6721 ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag
     6781 acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact
     6841 tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc
     6901 agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac
     6961 gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg
     7021 atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg
     7081 agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg
     7141 ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc
     7201 agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc
     7261 acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat
     7321 tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc
     7381 taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca
     7441 agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc
     7501 cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa
     7561 aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat
     7621 cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat
     7681 tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat
     7741 cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa
     7801 aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg
     7861 cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc
     7921 acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt
     7981 gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt
     8041 cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac
     8101 aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg
     8161 aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg
     8221 ttttttcgta ccttacaaaa aaagaagagc tgtggctttt gccgcggctc tt
//
LOCUS       DACEND010000109         8270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEND010000109 DACEND010000000
VERSION     DACEND010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.112"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            197..1390
                     /locus_tag="IW746_002118"
     CDS             197..1390
                     /locus_tag="IW746_002118"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1079010.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1520..2992
                     /locus_tag="IW746_002119"
     CDS             1520..2992
                     /locus_tag="IW746_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1079011.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3075..3863
                     /locus_tag="IW746_002120"
     CDS             3075..3863
                     /locus_tag="IW746_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1079012.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3930..4598)
                     /locus_tag="IW746_002121"
     CDS             complement(3930..4598)
                     /locus_tag="IW746_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1079013.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4713..6425
                     /locus_tag="IW746_002122"
     CDS             4713..6425
                     /locus_tag="IW746_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079014.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6428..8200
                     /locus_tag="IW746_002123"
     CDS             6428..8200
                     /locus_tag="IW746_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079015.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2531 a         1385 c         1818 g         2536 t
ORIGIN      
        1 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
       61 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      121 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      181 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      241 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      301 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      361 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      421 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      481 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      541 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      601 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      661 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      721 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      781 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      841 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      901 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
      961 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1021 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1081 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1141 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1201 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1261 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1321 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1381 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1441 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1501 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1561 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1621 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1681 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1741 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1801 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1861 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1921 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     1981 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2041 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2101 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2161 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2221 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2281 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2341 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2401 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2461 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2521 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2581 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2641 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2701 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2761 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2821 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2881 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     2941 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3001 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3061 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3121 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3181 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3241 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3301 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3361 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3421 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3481 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3541 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3601 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3661 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3721 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3781 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3841 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3901 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     3961 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4021 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4081 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4141 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4201 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4261 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4321 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4381 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag
     4441 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4501 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4561 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4621 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4681 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4741 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4801 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4861 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4921 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     4981 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5041 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5101 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5161 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5221 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5281 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5341 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5401 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5461 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5521 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5581 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5641 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5701 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5761 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5821 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5881 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     5941 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6001 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6061 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6121 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6181 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6241 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6301 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6361 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6421 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6481 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6541 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6601 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6661 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6721 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6781 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6841 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6901 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     6961 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7021 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7081 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7141 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7201 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7261 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7321 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7381 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7441 aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7501 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7561 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7621 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7681 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7741 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7801 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7861 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7921 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     7981 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8041 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8101 tgatggggaa gtcattgaag aaggcagtca tcatgagtta ttagcagaaa atggatttta
     8161 tgcggaactt tatcataatc aatttgtatt tgaaagttaa attaaaagaa cctcgttata
     8221 acggggttct ttttatatat tttatgttgt ttgtttttat aaggggcaga
//
LOCUS       DACEND010000110         8261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEND010000110 DACEND010000000
VERSION     DACEND010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.113"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..2031
                     /gene="yfcC"
                     /locus_tag="IW746_002124"
     CDS             292..2031
                     /gene="yfcC"
                     /locus_tag="IW746_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1079016.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2188..3372
                     /gene="iadA"
                     /locus_tag="IW746_002125"
     CDS             2188..3372
                     /gene="iadA"
                     /locus_tag="IW746_002125"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1079017.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3483..3686
                     /locus_tag="IW746_002126"
     CDS             3483..3686
                     /locus_tag="IW746_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1079018.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3787..4449)
                     /locus_tag="IW746_002127"
     CDS             complement(3787..4449)
                     /locus_tag="IW746_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079019.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4462..5964)
                     /locus_tag="IW746_002128"
     CDS             complement(4462..5964)
                     /locus_tag="IW746_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1079020.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6512..8191
                     /locus_tag="IW746_002129"
     CDS             6512..8191
                     /locus_tag="IW746_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1079021.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2601 a         1449 c         1766 g         2445 t
ORIGIN      
        1 tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt
       61 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
      121 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag
      181 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
      241 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
      301 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
      361 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
      421 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
      481 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
      541 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
      601 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
      661 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
      721 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
      781 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
      841 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
      901 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
      961 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
     1021 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
     1081 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
     1141 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     1201 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     1261 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     1321 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     1381 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     1441 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     1501 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     1561 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     1621 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     1681 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     1741 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     1801 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     1861 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     1921 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     1981 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     2041 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     2101 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     2161 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     2221 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     2281 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     2341 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     2401 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     2461 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     2521 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     2581 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     2641 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     2701 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     2761 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     2821 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     2881 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     2941 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     3001 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     3061 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     3121 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     3181 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     3241 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     3301 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     3361 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     3421 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     3481 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     3541 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     3601 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     3661 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     3721 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     3781 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     3841 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     3901 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     3961 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     4021 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     4081 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     4141 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     4201 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     4261 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     4321 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     4381 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     4441 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     4501 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     4561 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     4621 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     4681 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     4741 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     4801 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     4861 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     4921 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     4981 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     5041 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     5101 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     5161 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     5221 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     5281 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     5341 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     5401 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     5461 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     5521 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     5581 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     5641 taaattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     5701 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     5761 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     5821 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     5881 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     5941 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     6001 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     6061 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     6121 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     6181 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     6241 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     6301 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     6361 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     6421 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     6481 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     6541 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     6601 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     6661 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     6721 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     6781 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     6841 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     6901 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     6961 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     7021 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     7081 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     7141 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     7201 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     7261 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     7321 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     7381 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     7441 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     7501 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     7561 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     7621 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     7681 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     7741 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     7801 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     7861 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     7921 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     7981 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     8041 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     8101 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     8161 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     8221 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat t
//
LOCUS       DACEND010000111         8221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEND010000111 DACEND010000000
VERSION     DACEND010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.114"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..421
                     /locus_tag="IW746_002130"
     CDS             155..421
                     /locus_tag="IW746_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1079022.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            565..2556
                     /locus_tag="IW746_002131"
     CDS             565..2556
                     /locus_tag="IW746_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1079023.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            2786..4087
                     /locus_tag="IW746_002132"
     CDS             2786..4087
                     /locus_tag="IW746_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1079024.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(4217..5587)
                     /locus_tag="IW746_002133"
     CDS             complement(4217..5587)
                     /locus_tag="IW746_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1079025.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5769..6092)
                     /locus_tag="IW746_002134"
     CDS             complement(5769..6092)
                     /locus_tag="IW746_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1079026.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(6417..7022)
                     /locus_tag="IW746_002135"
     CDS             complement(6417..7022)
                     /locus_tag="IW746_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1079027.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            7352..8068
                     /locus_tag="IW746_002136"
     CDS             7352..8068
                     /locus_tag="IW746_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1079028.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            8081..>8221
                     /locus_tag="IW746_002137"
     CDS             8081..>8221
                     /locus_tag="IW746_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1079029.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KAL"
BASE COUNT         2591 a         1503 c         1702 g         2425 t
ORIGIN      
        1 ttcacggtgt tccagaagca gcttctacgg aaataagctg aacacttttg ttacaacttc
       61 tttttttctg gagtttcttg gcgcatattt ttggagtcct tctgaataaa gcgtaaaata
      121 aaagtgagaa atatggataa aggagggaaa ggagatgctg aattttttat ggtcgttatt
      181 tatcggcggt atattaggtt tgatagctgg cgcaatttta ggaaaggaag tcccgggagg
      241 cgtatctgga aatatcatta tcggtttttt aggtagttgg ctcggcgaat ttttactagg
      301 accactggga ccggaactcg gcggttttta tttgatacca gcattattgg gtgcaatttt
      361 ttgccttgct ctttattcat ttgtggcaag acgtatcaga gaacgtccaa agaaagaata
      421 attttattca aataagttga cacttcagtg tgttacttgg ataattagaa gcaatttagg
      481 aaagcgggtg tcatggataa gaatatcatc tatcattcaa gcttacctaa aggatatcca
      541 tataacaaga aatgagggaa aaacgtggca acaacgcaca ataccaaaaa gatatcaatg
      601 gctggcctgc ttgtagcgat gggagtcgtc tacggagaca tcgggacaag tccgctttac
      661 gttatgaaat cgattgttga agataatggc ggattgtcag gaatcaatcc tgattttgtt
      721 atcggatcag tctcattgat tttttggaca ttgactctac tgacaacaat caaatatgtc
      781 gtgattgcat tgaatgcaga taaccatggt gaaggtggaa ttttctcact ctacacactt
      841 gtccgaaaag gtgggaaata tttgatcatt cctgcaatga tcggaggagc tgccttacta
      901 gcagatgggg tattgacccc tgctgtgacg gtaacgactg cgattgaagg cttgcgagga
      961 attccgcagt tttttgaccg gttcggtaat aaccagaacg tgatcgtcat tatcacttta
     1021 gttattattt ttgttttatt catggtccaa cgctttggta cagatattgt cggaaaagca
     1081 ttcggaccaa tcatgttcgc ttggtttact tttttaggcg taatgggatt aatgaacttc
     1141 agtcaagact ggacagttat ccgtgcactg aatccttatt atgctatcca gctgcttttt
     1201 agtgcagata acaaattagg cttgttcatt cttggaaata ttttcctggc tacgacaggt
     1261 gcagaggcgc tttattccga tcttggacat gtcggcaaac gaaatattcg tctgagctgg
     1321 ccttacgtca aaatttgttt ggttatcaat tattttgggc aggctgcgtg gttgttgaat
     1381 gtttatcaaa accctgaagc acagcaaatc aagaacctga atccattctt ccaaatgatg
     1441 cctcaaagtt ggatcgtgat cggcgttgct tttgcaacag tcgctgcagt cattgcttcg
     1501 caagcattga ttacaggttc gtttacgcta gtttcagaag caatcaagtt gaaactgctg
     1561 cctcggttaa agatcatgta tcctgggaac agtatcgggc aaatgtatat cccagcagtc
     1621 aatctgattt tatggatcgc ctgttctcta gtggttgtca cattccgaac gtctcatcat
     1681 atggaagctg cttacggtct atcgatcact gtgacgatgt tgatgacaac agcactgctt
     1741 tacttttatc ttatccaaaa tgggcattct cgttggcttg catatttagt tacctttttc
     1801 ttcggtgcga tagaagtcgt tttctttatc tcaagtgttg tcaaattctt ccgaggcggt
     1861 tttgtagccg ttctgattgg tttgatcatc ttagtcgtga tgtttatttg ggaacaaggg
     1921 aacattattc gcgaatcgtc aaccgaggat gtgtctttga gagattatat cccgcagctt
     1981 agagaattac gtgatgatac ttctttacca atctatcaga caaatgtggt cttcttggtt
     2041 cctgatatgg ctgaagataa agttggccga cagttcatct attcgatttt agataaacgg
     2101 cctaaaagag cacgagtcta ttggtttgtt catgtggaag tgacggatga gccatatacg
     2161 aaagaatatc aagtagatat gatggacact gattttattg tccaagtgaa tctgttctta
     2221 ggtttcaggg tccaacagga aatcaacgta tatgttcgcc aagtcatcca tgatttgatg
     2281 aaacaaggcc gtttgccaaa acagccacaa agatactctc tgacaccagg gcgagaagtt
     2341 ggcgatttcc aatttgtcct gattgatgaa gtagtttcca atgtgacaac tctaggaaaa
     2401 tggcaacgtc aaatcatgca ggcaaaactg gcaatcaaaa aaatcgctac ctcgccagaa
     2461 acatggtttg ggctagagta cagcgaagta aaacatgaaa ctgttccgct tatcattgga
     2521 aatactcgga aaacatggtt gaaagagcgg ttgtgaaagt agcgctttga cctgaactaa
     2581 tgtcgaatgt cagctacttg aacaccgttt atcagaggtc atgaaaaagc tgtttagcaa
     2641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
     2701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
     2761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
     2821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
     2881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
     2941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
     3001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
     3061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
     3121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
     3181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
     3241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
     3301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
     3361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
     3421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
     3481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
     3541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
     3601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
     3661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
     3721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
     3781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
     3841 agatggctct tatatcatct ccggcaaact aacattagat gatttccagc gttattttca
     3901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
     3961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
     4021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaat
     4081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttgtta tctaaaaaac
     4141 ctgttttcgg taaacgaaga agatattttc aataaaagtt ttcattttta gccgcaacga
     4201 tttcttttaa tcaaggctat cttgcttctg catgttctaa tgtgaaatgt tcctcttcac
     4261 ccatgtgttt caattgtttc atcgaacgca tataagagta aagcaacacg gcaacagaac
     4321 cgatccaagc taaaggagaa gctgctgctg ctcctgggta tccccacatt cctgtaagaa
     4381 cgatagctgc aaaagaacgc ataagcaatt ccatcatacc ggcgatcgta gggatcttgc
     4441 tttgtccaag cccttgaagt gtgtagcgta aaatgaacag gactgctaag atccagtaaa
     4501 gagacccatt gatattgaag taggtttgtg ctaattcaaa tacacgtgtt tctgatgggc
     4561 taacaaacaa ttgaacaaag aaatgaccga agaaaatgac gatagcacct gctaagagac
     4621 taaatccgac actcatcagt aaagtttgac gtaccccttg caagattcgg ccatattctt
     4681 tcgcaccata gttttgtgca gtgaaagttg ccattgcaat accaaatgac atcatcggct
     4741 gatttgcaaa ttggtcgata cgaccagcag cggcttgagc agctacgaca tctgtcccta
     4801 gactatttag tgctgcttgt aacacgatag caccaacagc gatgatcgaa gattgaaacg
     4861 ccattggcaa agcagcattt aggtgtacag caaactcttc tttttcaaat ttgaaatctt
     4921 ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata cactagacac agtacgctgg
     4981 aagaaacttg tgcgattact gtagcaatag ctgcaccttc cacacccatg ccaaaataga
     5041 taataaagat caaatcaagt acgacgttga tgatgaccgc aatgatcaga aagaatagtg
     5101 gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag caaattgaaa ctgactgatg
     5161 caaagatccc taataaaata attgaaataa atgtttgtgc ttgatcaagt atttcaggag
     5221 gagtttgcat caattgaagc atcggacgaa taaacaggag acttaggaca gttaaaatca
     5281 cagtgacgac aagactaatg acaacactag cagcaaaact tttcttcaga ccccgataat
     5341 cttttgcacc gtaacgttgt gcggtaataa tggctaaacc agctgttaaa ccttgggcaa
     5401 agccaatgat caaaaatgtc aggcttccag ttgcaccaac cgctgccagc gcatttttac
     5461 ctagcgtctg acccacgatg atcatatcga tcatattgta gaactgctga aaaacatttc
     5521 cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc ggggcttcct tttgttaaat
     5581 cgcgcatctt ttaaactcct tatatcttta aaatttagtt agtagtatat agcgtttatc
     5641 ctgagaatac caattagaaa tcaatgataa tttatgaaac tccataaaaa gaagccaaga
     5701 attgagtcaa agaatcaaca acttggcctg gtctgtaaaa taagggacat ccacgtttat
     5761 ctatcacctt aatattgtct aatctcactt ggtaaaagga tcgtttctct cgtaccgtca
     5821 tcataggcgt atacttgagc aggaaattgg ttcgaatagg caaacggcaa atcaatatcc
     5881 atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca cttttccacg attttcgatc
     5941 aaatcaaagg ttaatatatt ttctaatgga atgactccct taagatctag gtcaataata
     6001 aaccatatac tgtcaatcaa ttcatctggc aaacttgata cgacaccaac tgaagcgtaa
     6061 cggttgcggc tgctatcaaa actttcaaac atctgtttaa cctccttcga atcaacgaat
     6121 gttttatttt tataattaat cctaggataa actagaaata cttacttcaa cgagggttca
     6181 ttctaataaa gaatcaggaa aaatgcaaca ataattgaat actttttttc aaagtataaa
     6241 tttttataaa ggtacgataa aagtatgcga ggaaaagctt ggtaaattag gatttttcgt
     6301 ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa taaacgatta gaaataaaaa
     6361 aggcgtgtta aaagctgctg acccgccgct tctaacacca cctgattgtt tcagtattat
     6421 tcttctgttg aaccgatcac ttctggttcg ccaccttcac ctgtgctttc ttcttcactt
     6481 tgagcgacta cagcagcgat ttgctcttca ctgtcggtca cgaatttgta atcgtcgttt
     6541 tttggaagat cggctactgt aatagaatca cctaattcca agtttgtcac gtcaacttca
     6601 attcgttcag gcaatttgtc tggtgtagca gatacgacta ctgtatacaa gttttgagct
     6661 aacactccac cagatttagc acccactgat tcgccaacaa gaacgacttc tgtttcgact
     6721 tctgtttctt ccgacatgtt tacagataga aattctgcat gttcgaaatg attggtaaaa
     6781 gtgttcgttt ggatcttgtg aaccaatgta ttgacttttt gtccatcgat gtccatcgtg
     6841 atgactgtgt tcgctccatg ttcacgcaac aatttgctta gtgtcaaacc atcgaatgag
     6901 ataggggtac tttctacttt atacccataa acaacagctg gaactttccc ttcgtgacga
     6961 agtttgtttc ttaatgaacg gggacgtact tctcttttac ttacttctaa tgatactgac
     7021 ataaccaaaa ttcctcctta aaatgtgact gtctgctttg gtaacggaca gattttattt
     7081 aagtgaattc taaaggctaa tctcgtgcac atcatcgaag gcagggcgct taaaaaagcg
     7141 caaaaggaga gatgcgcgca tctcccctta gaaagtcagg attcaacttt tctccaccag
     7201 gtcattacgc tgtgtatgag aaatccaatt cactttacta tttaaccgta acgtgaactg
     7261 ttaattaagt caaggaataa ctatgatgat gtatttgcaa gcaagaatat tcaaggtata
     7321 attaattagt agtaataaga aaggaaacag catgcgttta gataaattaa tagaagaaaa
     7381 attagaaacg acacgcaagg aaatgaagcg gctgtttgct ttgaagcaag tgatgataga
     7441 tggcacagtc gaatttcgac aaaatcgaaa tgtagatagt ttactgcatc aaatcgaagt
     7501 agcaggtgaa cacttagaaa caaatgagtc ctattatctt ctcaataaac ctgaaaaagc
     7561 tgttacagct gtatcagatc cagaaaagac gacagtaatc gatctgattg caagtgccga
     7621 tcgtttccaa ccattatatc cagtgggaag actagataga gatacaacag gactattgct
     7681 gatcacttcg aatggacaat tgggatatga aatgctactt ccagaaaaaa aggtttcaaa
     7741 gacttatcaa gtagttgtga acgaaagggt gacaccggca gacgtaacag catttgaaaa
     7801 tggaatcgta ttcgctgatg ggtatcaatg taagccggct gatttaaaaa tactcaaagc
     7861 agacaagcac caaagtacgg ttttattgac gatccaagaa ggaaaattcc atcaagtcaa
     7921 aaagatgttc ttagcccaag gaaagaaagt gaccgcattg aaacggctaa gcatgggacc
     7981 attggaatta gatgaacagc taggagtagg cgagtatcga tcgttgacat tagcagagat
     8041 gaaaaagtta agtatttatt ttaaatagga ggtcaaaaag atgaaatata aaactttatt
     8101 gttcgatgta gatgatacat tgttagattt tcaattaact gaagccaatg ctttaagtgc
     8161 tttgtttgca gaacaaggaa tcacattgac accagaaatc gaaaccagct ataaggcttt
     8221 g
//
LOCUS       DACEND010000112         8119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEND010000112 DACEND010000000
VERSION     DACEND010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.115"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..973)
                     /locus_tag="IW746_002138"
     CDS             complement(131..973)
                     /locus_tag="IW746_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1079030.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1010..2476)
                     /locus_tag="IW746_002139"
     CDS             complement(1010..2476)
                     /locus_tag="IW746_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1079031.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2532..4376)
                     /locus_tag="IW746_002140"
     CDS             complement(2532..4376)
                     /locus_tag="IW746_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1079032.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4978..5928
                     /locus_tag="IW746_002141"
     CDS             4978..5928
                     /locus_tag="IW746_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1079033.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5973..6606)
                     /locus_tag="IW746_002142"
                     /pseudo
     CDS             complement(5973..6606)
                     /locus_tag="IW746_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6866..7414)
                     /locus_tag="IW746_002143"
     CDS             complement(6866..7414)
                     /locus_tag="IW746_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079034.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7415..>8119)
                     /locus_tag="IW746_002144"
     CDS             complement(7415..>8119)
                     /locus_tag="IW746_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1079035.1"
                     /translation="NLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVIN
                     NLYILPADMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS
                     KSAMYIADWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAV
                     DQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFIS
                     ILKELEEHENWYAEVE"
BASE COUNT         2424 a         1651 c         1288 g         2756 t
ORIGIN      
        1 agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata acaaacagct
       61 ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca tcaatagtat
      121 tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca cctaggtata
      181 caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct ttgatcttag
      241 ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat tcttcctcac
      301 catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca tgtgtaatga
      361 agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac cggaccaaat
      421 tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca ggatcttccg
      481 attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga agatcaattc
      541 catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct ttgggatagt
      601 agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt gcgcgttcaa
      661 ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt tttttatcta
      721 atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca ataggtattt
      781 cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga aatattttat
      841 ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc cctttaccta
      901 atgcaatcat ttctttgcca ttttcttcaa caagtaccac acttgaattc aatacttttt
      961 ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac tactcgaata
     1021 aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc tttttcaagc
     1081 ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca tatcgtttag
     1141 acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta taagctatca
     1201 aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta aagtagtcga
     1261 tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc ataccatttt
     1321 ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa attttcaaac
     1381 cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc ttaaccccta
     1441 aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc atggacatgt
     1501 aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga tcttcatctt
     1561 ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca ggatattctc
     1621 ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag gtcagctcaa
     1681 cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata caaccaactt
     1741 ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca acaaattgat
     1801 gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc tcttggataa
     1861 ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat gtcagccaat
     1921 attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca aaatcatcaa
     1981 ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc atttcataat
     2041 gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag acgttttcat
     2101 aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac aagcgagtcc
     2161 aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct aaatcttctt
     2221 tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca gatttgtctt
     2281 cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa tctttgttgt
     2341 ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg tctacattcc
     2401 aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct tttggaaaac
     2461 ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata ttttttttca
     2521 ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt ggttaccaat
     2581 ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac acagataact
     2641 gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag cttttgtcct
     2701 ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc taactgaaca
     2761 gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa accaatagca
     2821 tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa tatcccttct
     2881 tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt gtcttttact
     2941 tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc tgaaacaacg
     3001 gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata aagaaccaac
     3061 gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca aaccagattc
     3121 attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc cactaaagca
     3181 aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga aaacataacg
     3241 gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc agtaattccg
     3301 aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc ttttgttttg
     3361 atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga agccgggaga
     3421 taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat cattgcttta
     3481 tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt tgcaacccat
     3541 ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac attataacct
     3601 aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat cataggtaca
     3661 aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag ttgagcataa
     3721 aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt atccaatcca
     3781 aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag gatcaatgca
     3841 ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt agtcattgcc
     3901 accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat ctgataggtt
     3961 tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga cttcaatact
     4021 cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa atctaataga
     4081 actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac attgtcacct
     4141 aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat tacttggcat
     4201 tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct catatttacc
     4261 ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa atgggatatg
     4321 ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta attcatggtg
     4381 tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat cctcaacaca
     4441 aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga aacgcataca
     4501 ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg tttaagttat
     4561 attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt aatcattttt
     4621 tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt ttctaagaat
     4681 atcttccaac atatataact cttattctct tctatcttct agcagatgtt ctgtcttttt
     4741 aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga ggttttttat
     4801 ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta ctaaaaaatg
     4861 ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata tcgatattgc
     4921 ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt ttataagatg
     4981 aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat cagtaattta
     5041 gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg aagaacaaat
     5101 gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat caatactcgt
     5161 ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc ggtaaaagat
     5221 gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac agattccatt
     5281 ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca aatgaaaact
     5341 gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac tggagttgga
     5401 aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt tattcatgag
     5461 aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga attattgtcg
     5521 gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga tcgaaatgga
     5581 ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag attgcctcct
     5641 gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag agtcggtgaa
     5701 actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg caaagaggat
     5761 tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa tattgaggaa
     5821 cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga aagcaaaaac
     5881 tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat atagtcagaa
     5941 tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa actttcatct
     6001 acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac cgctaccttc
     6061 acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc tcttaaatta
     6121 gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg tatttggctc
     6181 ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat aattgaccat
     6241 atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac
     6301 taataatact tgatctcgct tattataatc gctttcattt gtcctaagag tcaaatcttt
     6361 ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct
     6421 ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt gagagtcatc
     6481 taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc
     6541 catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt
     6601 tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta cttatttact
     6661 aactcaacaa cagattatca tacacttatt aatgaaatca caatatctca ttaaaacaca
     6721 ccctaaaatc ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa
     6781 aaataaatcc cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg
     6841 tagttactta gttcttcttt gattattatc taactttatt attcattccc caccctttgc
     6901 gcagtgagtc ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat
     6961 catctaattc tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt
     7021 cattaaacaa atcttgttta tcttcttgcg taagattctg tttaatatac tcatctaaag
     7081 cttttctaag caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg
     7141 aagtcgcatt taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa
     7201 actttctgat tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg
     7261 aggaagtttt tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa
     7321 ttgcttgctg ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt
     7381 tcccaaaaat atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct
     7441 cgtgttcttc caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt
     7501 gttttaagag acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta
     7561 agcgcgcatt tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt
     7621 gtttatacat ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag
     7681 caacatataa atttgtatca aattctttca caaaaaagtc catatactca agatattgca
     7741 tcgcatttct catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt
     7801 acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa
     7861 actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat
     7921 tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat
     7981 agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt
     8041 cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga
     8101 agttcatatg cgttaagtt
//
LOCUS       DACEND010000113         8097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEND010000113 DACEND010000000
VERSION     DACEND010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.116"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW746_002145"
     CDS             complement(310..807)
                     /locus_tag="IW746_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1079036.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW746_002146"
     CDS             complement(847..1656)
                     /locus_tag="IW746_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1079037.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW746_002147"
     CDS             1990..2847
                     /locus_tag="IW746_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1079038.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW746_002148"
     CDS             2840..3742
                     /locus_tag="IW746_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1079039.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW746_002149"
     CDS             3775..4125
                     /locus_tag="IW746_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1079040.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW746_002150"
     CDS             4159..5097
                     /locus_tag="IW746_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1079041.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW746_002151"
     CDS             5175..6068
                     /locus_tag="IW746_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1079042.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW746_002152"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW746_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1079043.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IW746_002153"
     CDS             6683..6871
                     /locus_tag="IW746_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1079044.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IW746_002154"
     CDS             6884..7786
                     /locus_tag="IW746_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1079045.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IW746_002155"
     CDS             7818..8060
                     /locus_tag="IW746_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1079046.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2804 a         1414 c         1585 g         2294 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttct
//
LOCUS       DACEND010000114         8054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEND010000114 DACEND010000000
VERSION     DACEND010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.117"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..220
                     /locus_tag="IW746_002156"
     CDS             <1..220
                     /locus_tag="IW746_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1079047.1"
                     /translation="QAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPY
                     ANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            253..1215
                     /locus_tag="IW746_002157"
     CDS             253..1215
                     /locus_tag="IW746_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1079048.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            1208..2641
                     /locus_tag="IW746_002158"
     CDS             1208..2641
                     /locus_tag="IW746_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1079049.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            2638..3708
                     /locus_tag="IW746_002159"
     CDS             2638..3708
                     /locus_tag="IW746_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079050.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3695..5116
                     /locus_tag="IW746_002160"
     CDS             3695..5116
                     /locus_tag="IW746_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1079051.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            5129..6136
                     /locus_tag="IW746_002161"
     CDS             5129..6136
                     /locus_tag="IW746_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1079052.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            6148..7152
                     /gene="neuB"
                     /locus_tag="IW746_002162"
     CDS             6148..7152
                     /gene="neuB"
                     /locus_tag="IW746_002162"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1079053.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            7149..>8054
                     /gene="neuC"
                     /locus_tag="IW746_002163"
     CDS             7149..>8054
                     /gene="neuC"
                     /locus_tag="IW746_002163"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1079054.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVI"
BASE COUNT         2902 a          981 c         1367 g         2804 t
ORIGIN      
        1 acaagcagca aaattaaaat tagtcttagc agatactatt tccaaagata caaagttaac
       61 aaataatgtg gattttgtat cattagatga aacaccacaa gaatgggcaa aaaaagtaat
      121 aaaagagccg tatgctaata aaataacaca agaactaaag agttatgatg tttcgtacac
      181 tactgaacaa atggagagta tatatagtag agatcattaa aaatgttcaa gatattttag
      241 gagtgattat ttttgaaaat atgttatttg atattggcat acaatgatat tggcaactta
      301 aagagattgg tcaatcgatt gaacgaaaat gcagttttta ttattcatat agataaaaaa
      361 actgatatag aaccatttat tgaagcattt aaatcatatg ataatgttga atttttatgt
      421 aataaaaacc gtgttaaaat ttattggggt ggtttttcca ttgtacaagc agaaatcaat
      481 ttagtaaaga gagcattaca aaatgagaaa tatttaaaat atgtattatt atctggtgct
      541 gattatccaa tcaaagataa tgagtatatt tataattatt ttaaaaagaa tagttcagtg
      601 gagtttattc gtggaataga tctagatcaa attaaacata aagaatttta ttataaacat
      661 atagatgtat atcaaaaaca tgattatcct agaattaata ggaataacac aactgcattt
      721 aaaattttta gagctataat taatcgttgt ctgaggatga ttaaacttcc cccaaaaatt
      781 agacaccata aatttgattt gtatcatggt tctcaatggt gggctttgtc gaaagaatgc
      841 ctaacagaat taatacaaat gtatgaacaa aatcaacaag actacctgaa ttttaaaata
      901 ggtatgtttg ccccagatga aaaatttttt catacacttt tttttaattc ctcgttcaaa
      961 aataaaaatg ttattggagg accagattta ccacttgaat tgaaaaatat tgaagaaact
     1021 actttacaaa cttcgaaatt agcaaatatt catattattg atccttccat gaacaaatgg
     1081 tttgatgaaa gggatataga tcaaataaaa gaaagtgaaa aattatttgt aagaaaagtt
     1141 aaatcggatt actctgataa actattaaat agaattgata aagaaatttt gaaagttgga
     1201 aatatagatg aatagaatag tacctgtttt agagaagagt ttattaagtc gagatgcgtt
     1261 tcgagtaata aatttaatca tttgtctgct ttattttatg cctagtactt ttaatgtaac
     1321 taatatcccg atgaaaatag cttttgcttg gggtggaatt atagttttgt atgaggtttt
     1381 tataaagaaa aattttttta aaatgcgttt ttcttggcta ctgattggag caatgattag
     1441 ttttatctta actataatca ttaacagaga aaatttttta attccatcta tttataattt
     1501 tggatatttg ctgattactt tgatagtaat ttttcctgca gacttttcac aaagtgaaga
     1561 ggaaaaaaag aaaaaaatgg tgtattttaa taatgttttc attatcataa tttttttagc
     1621 tgcgctaata tctatctatc aatttatttt tttgatttct tatcatgtcc caacaggaac
     1681 gccaggatta cttgcaagac aaggttttat tgaacatcgg ttatttggtg tctatacaag
     1741 tccaaatgtt ggagctatgt ttggatatac ttctattatt ttaagtttaa ttagtgtatt
     1801 aatttcaaaa ataacaaaaa cgattcgtgt tttttatatg atgaatttta tcgtacaaat
     1861 attttatatt acactttctt cgtctagagg tgcccaggta gtaatttgtt cttttttagt
     1921 attatttctt ttgttgtttg ccaattcaaa atttagaaag agcattagag aagtactacc
     1981 tgtaaaaatc aaatggatgg taagcatatt attagttcta ttggtatatt tttcttttgg
     2041 tactaattat gctaaggatt ttttagcaac aattcctgta tcgattgata gaaaagtaga
     2101 agttatagag tcaaatgaat cagataaaac tccacttcca gaaaaagaag taatattaca
     2161 gcattctagt gataactccg aaatttcagc tggtcgtttt acgatttgga aagctgcgct
     2221 taaagttata agaccaactt tgttatttgg ttatggagag accgatttct atgggaatag
     2281 cgatgcaaaa tttttgaaaa aagtagatct tgatactaca gatgtgaatg aactaaaaag
     2341 agcacatgga aacatgcata atggatttct tcaagtactt gtatcaagtg gtataattgc
     2401 ttttatatgt atctatgctt tctatgtgtt aaatatcttt agtttactca aaacattttt
     2461 tagaagtaaa gtgaattatg cgtttcttgg tattgtttta atttttatac tatctatttt
     2521 tatagatgaa atggtggagg ctcatgtact tttcaataag cgcgatgtca tttcaattgt
     2581 tttctggtat tatttgggaa gcatttctta cttgtattta aaaaaaggag aattaatatg
     2641 aagacagctt tcattgcttg gactccttta catttgatta atatattaaa tgtttatcaa
     2701 aatttttacc gagaatcaaa aggagattta tttatttatt ctgaatttga aaacgctaga
     2761 agatatagtg agcgtataaa agaattaggg ctatttaatg atgttgtttt tgtgaatcac
     2821 cggaaaatcg gttcttcctt taaacgtaaa attaacttgt taactaacca taatcaattt
     2881 ttctcggatg atttatccgt ttatgatgaa atttttatac agggggaaaa ttatttcaca
     2941 aaaatacttt tctccaatgt ttacaaaaaa aatccagatg taaaactaaa ttatatagaa
     3001 gatggtttgg gtacttattt gaagaagagt atattttcaa caagtaaaaa cgttcaaaga
     3061 gtttttaaaa tattaaataa aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc
     3121 tattgtccgg aattgttact ttatgatgta aaaaatataa aaaaaattcc caaaatagat
     3181 gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga agtttttgtc
     3241 ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg catagatgaa
     3301 acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac tttaattaag
     3361 cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa ttccagttta
     3421 ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat tagtcctata
     3481 tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc tattatgttg
     3541 gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca
     3601 aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca
     3661 gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa
     3721 gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat
     3781 ttcattcata actattccta tttttgtaaa tattactact caagcagaat atggtttgat
     3841 taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc
     3901 ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt
     3961 actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt
     4021 gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt tattacaaag
     4081 ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact ttaaccattt
     4141 taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct ctgtagcttt
     4201 gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg gaacactgtc
     4261 atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg ctcatttttt
     4321 tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc cacattcctt
     4381 agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt taggtcctgc
     4441 tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg taatatggat
     4501 gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa
     4561 aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta cgatgattgt
     4621 aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt attctgggtt
     4681 atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata gtttaccagt
     4741 taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag tagcaagtgc
     4801 tatagttaat atactattaa acatgatttt catacctagg ttaggaataa ttgctgctgg
     4861 atacacaacc gtatttacgt attttttact ttttattttt cattataata tcgctaaaaa
     4921 aattacgaat aaacaattat ttgacacaaa aaaaatatgt atcataataa tttttgtatc
     4981 aattttttct ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac
     5041 atcattattg ataattagtt tagttatata ttttttaaag attaagaatc gaattataga
     5101 tgactataat tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg
     5161 cggaactggt tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc
     5221 tatcagaatt tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa
     5281 tcacactaat ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc
     5341 atgtcgtgat gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga
     5401 atataatcct atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc
     5461 agcgatcaaa aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc
     5521 tactaacacc atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca
     5581 gtataaagga aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc
     5641 aagtggatct gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt
     5701 gacagatgtt gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct
     5761 gtctgctgct atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa
     5821 tattttaact ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata
     5881 tagaggtgaa ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat
     5941 gactacagac gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca
     6001 ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc
     6061 ttcagatgca acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa
     6121 tgtcgtgact tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt
     6181 ggtgtaaacc ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa
     6241 tgtggagcta acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag
     6301 gctccaatgg caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg
     6361 ctaaaaaaat tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa
     6421 aataaaattg attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa
     6481 attggtgtac aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga
     6541 aaaatagata tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag
     6601 gtcagtgagg ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca
     6661 aattatccgg ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg
     6721 ttcgggatac ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct
     6781 gttgcgatgg gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt
     6841 ccagatcata aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga
     6901 aacactgaaa tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca
     6961 aaagaagtat caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt
     7021 acagagaaat ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat
     7081 caatatattg gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa
     7141 gatgttgaat gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata
     7201 aacctgttgc tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa
     7261 tgcatttatc ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa
     7321 ttagtggaga agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg
     7381 tggcattaga gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat
     7441 tattactagg ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa
     7501 tcaaaacagc acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac
     7561 atgccataac gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt
     7621 taataaaaat gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata
     7681 ctattttaaa taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat
     7741 ttgaaaagaa aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata
     7801 tagaaaaaca aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt
     7861 gcattttacc taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt
     7921 taaaaacgga tattatttat attacgaatt tagagccgga ggaatacctg actatattaa
     7981 aagagtgcaa atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca
     8041 aaataccagt aata
//
LOCUS       DACEND010000115         7801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEND010000115 DACEND010000000
VERSION     DACEND010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.118"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            211..996
                     /locus_tag="IW746_002164"
     CDS             211..996
                     /locus_tag="IW746_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1079055.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1009..1860
                     /locus_tag="IW746_002165"
     CDS             1009..1860
                     /locus_tag="IW746_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1079056.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1984..2436)
                     /locus_tag="IW746_002166"
     CDS             complement(1984..2436)
                     /locus_tag="IW746_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079057.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2901..3269)
                     /locus_tag="IW746_002167"
     CDS             complement(2901..3269)
                     /locus_tag="IW746_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1079058.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3419..3901
                     /locus_tag="IW746_002168"
     CDS             3419..3901
                     /locus_tag="IW746_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1079059.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            3938..4948
                     /locus_tag="IW746_002169"
     CDS             3938..4948
                     /locus_tag="IW746_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1079060.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5063..5776)
                     /locus_tag="IW746_002170"
     CDS             complement(5063..5776)
                     /locus_tag="IW746_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079061.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(5966..6556)
                     /locus_tag="IW746_002171"
     CDS             complement(5966..6556)
                     /locus_tag="IW746_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1079062.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7079..7750
                     /locus_tag="IW746_002172"
     CDS             7079..7750
                     /locus_tag="IW746_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1079063.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
BASE COUNT         2615 a         1269 c         1503 g         2414 t
ORIGIN      
        1 ttttgtaaat tacttcttat ttcttgaagc tggccacttc tgtcacaacc tctttcttta
       61 tagaaataaa aggacactag caattcatga aaacgattgc tagagcgtat catttgcttt
      121 agactatata tcttggtgta cactcacttt aagacggaaa taaaagttat ccgtaataac
      181 aggtttaggt atgaaaaagg agtgggtact atgtataagg aattagaaaa caaagtcgca
      241 gtagtaactg gtggctcaaa ggggattgga acagcgattg ctcaacgatt cgggaaagaa
      301 aaaatgaatg tagttgtcaa ctataactcg gataaagaag gagcagaaaa agcagtcaaa
      361 gcaatcgaag aggcaggcgg aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa
      421 ggaatacagc gtttacttga tacggcttta gaaaattttg ggacactgga cgtttggatt
      481 aataatgctg ggatggaaaa tcagcatcca acccatgaac ttagccttga agattgggaa
      541 agagtattaa aagtcaattt gactggcgtt tttatcggaa cgaaagcggc tttaaattac
      601 tttacagaga atgataaaaa aggaaatatc atcaatatgt cttctgttca tgaacaaatc
      661 ccatggccaa cgtttgctca ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa
      721 acggtagcaa tggagtacgc accaaaggga atcagaatca atagtatagg acctggtgct
      781 atccgtacac ctattaatgc ggcaaaattt gatgatcctg aacaaaaaga actcttggaa
      841 agtatgatcc cactaggacg cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg
      901 ttggcttcag acgaatccag ttatgttaca ggtattacac tatttgcaga tggtgggatg
      961 actttatatc cttctttcca aggtggaaaa ggataagggt gaataaaaat gggtttattg
     1021 attgcattga tcccggcagt tgggtggggg atccagccac tgattttgaa aaagattggt
     1081 ggcagaccaa caaatgagat attaggaact ggtattggag ccgtgctcgt tggcttactt
     1141 gttcaattat tcgcttctcc agctggtatt tcagtgacta catttttgat tagtttactt
     1201 tctggtgcat tctgggtcat cggacaaaca ggacagtata catctttcaa cttgattggt
     1261 gtttctaaaa cgatgccaat ttcgactgca ttacagttag tcggaacctc attgattggt
     1321 gtcttcgctt ttggagaatg gtctggaagt atgggaaaaa tcattggtgc agcagcaatc
     1381 attcttcttg tatttggttc tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc
     1441 ggtatcacaa aaggaatcag tctattagca tcaacaagta ttggttattg ggtttacagt
     1501 gcattgccca aactagttga tgcaagcgga ctttccattt tctttccgca aatgatcggt
     1561 gtctttttag gggcagtgat ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac
     1621 aagagttgga aagcaacaat agttggagcc atcttcagtg tgtcagcatt ggcttatatt
     1681 ttttcagcaa atgaaaatgg ggttgctact gcatatatca ttacacagct aaacgtagtt
     1741 atctctacat taggcggtat gtacatcctg catgaaaata agacaccaaa agaaaaaaaa
     1801 ttaacgatta tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga
     1861 ggataaagat ggtaaaatct catttattaa aaaaatattt gtttgaacca aacagtaagt
     1921 acttataaaa aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat
     1981 tgtttaagat acaagtgaaa tatctgtctg catcaataat ttttcattga tccattgact
     2041 aaagtttacg acataattga agttaccacc gattagtatc tccgcatgat tgtctttata
     2101 aagatcgtat tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac
     2161 taattgttct cggaattgat tgattgctgt aaaatcacga tactgaaggt tgaaacgcat
     2221 cgtgattggt gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc
     2281 tccgtatagg aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt
     2341 tccttttagt tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg
     2401 ttcggctaat tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt
     2461 tctactgact tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa
     2521 ggtcattgta aaaatcaagt cagaaaattc taacttttct ttctctaaag aaattcgttc
     2581 tctcctaaag tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga
     2641 tcactgatat tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa
     2701 aaataatttt actaagtgga aaattcaaag ccattcaatc agtgattttt ttcagcacaa
     2761 gaaagactcc ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat
     2821 aaaaaggata ggattgaaca aaacctgtat aagctgatga atagtttcct cgccagtaaa
     2881 aggtttattc gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata
     2941 ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg
     3001 atgacgttat ctgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc
     3061 gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag
     3121 gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac
     3181 gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatc
     3241 tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt
     3301 attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta
     3361 accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat
     3421 gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg
     3481 agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac
     3541 agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat atctgacttt
     3601 tactacgaaa caaacggatt tcaaagcatt ggggaagatc gtcggtaaaa ggctttctat
     3661 tgtttcatca gaacacatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt
     3721 tggttatgaa aaacagattc cgattattat tgatgaagag ctcttattaa atgaaaaact
     3781 tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa
     3841 aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta
     3901 accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga
     3961 aagaaataca acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc
     4021 gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa
     4081 gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt
     4141 caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgccttggg gaggagaatt
     4201 attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg
     4261 ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc
     4321 acatggaatc aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg
     4381 ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaacctt ctgaaaagta
     4441 tcaaaaagag tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac
     4501 aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggctg
     4561 tattgatacg atcagtcatt taatcggtac cccttatgga gatgtccaat cttttaaaag
     4621 aaagtataat gatggaaagc cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt
     4681 tgatttaaga agatcacttg ttcagatgaa actggcagga tggtttgatg gcagctcggg
     4741 gatcatgttc ggtagaagtg cagcagagga tttgttagat tataaaatga aggatgtata
     4801 cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt
     4861 accgccacaa ctgacattga tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa
     4921 agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tttatcaaag
     4981 accaacaaat ctaaatagaa aagaaaatga aagaagctga cagtattttg ccagcttctt
     5041 tttgtatgat ctattagaaa ttttaattat ccataactat cacattgatc agctgtttca
     5101 catcatttgg attacctgta ggaatagcta ctacagatgc attatcaata atttcttgag
     5161 ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgtag
     5221 gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt
     5281 aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat
     5341 aaggggcgat aggtgttcct gcaggataat gaataacatg tagttcgaaa tcactcgatg
     5401 tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat
     5461 cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaagtt gtgtctattg
     5521 ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg
     5581 agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg
     5641 atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac
     5701 tattttcttt actaggagac gtacatccta caaggaataa agagaccaac aataaaataa
     5761 aacctaactt tttcatataa aatacctcat tattttttta ataccagtat agcgtaatcc
     5821 tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtatat cttctttgat
     5881 aaatgtcaga aaaagtgtga caaataacca tcagtaaaat tacttgtcat actattgaga
     5941 aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat
     6001 catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat
     6061 ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca
     6121 aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg
     6181 attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct
     6241 gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt
     6301 tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa
     6361 taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc
     6421 ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat ccctcttttt
     6481 ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg
     6541 taatcgcagt tttcattttc aagctctcct ttactatcta tgacaaggac aacagtttta
     6601 acatacagac attcattaaa tttggatagc gacagcgaaa acaacaattc ttaagtttta
     6661 gacagagtat acagacagtc aacttaatta aatacgaact aaataaaaat gacaggtaat
     6721 catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc
     6781 cacttgcttg agtacaatct taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa
     6841 aaataaatca tatcgggcac tcagacatga aaacaaccct agaaatatat aactagtcca
     6901 atgaaaaaac agcgaatgcc gtagaaaaca aggattctgc gtacattcat atctcatttt
     6961 gcgagcatat ctgctattac tgtgactaaa tgttaatatg gttaaaatga ccgttatatt
     7021 ttttatataa atatcgtata attagtatga atatagaaga aggaaagaag gtgcaatctt
     7081 ggaacctaaa gacaagtatt tagattacgc tagggtcaat aaagataaat ttattgaaac
     7141 aatcatcaag ggaaaaatcc ctgatgatga aaaagatgcg gtattcatgg ctggaagccc
     7201 aggagcaggt aaaacagagg ttgcactagg tttagctgaa aattatgaca accatgttgt
     7261 gattgatgcg gactatttta gagcacaatt tccagaatac aatggaaaga actcaagtgt
     7321 ttttcagaaa gcttcttctt ggttagttga acaatcatta aaatatgtac tggaacatgg
     7381 ctattctttc atactagagg atacttttgc tatccttagt gcagagaaaa atattattcg
     7441 agcccttaaa aataactttc gcgttacgat tttctatgtt taccaagatc ccaaagttgc
     7501 ctgggatttc actcgaaaac gtgaactagc tgaaggacgt catgtgccaa aagaaatgtt
     7561 tatcaatgcg ttttttaagt ccagagagaa tattgaaaaa gtcaaaaata gacatcctga
     7621 agtagtcctt cacataatga ttaaggatta tcaaaacgac atttctgaag tgcattatgc
     7681 agcagataat attcaattag tactacccaa tacaatatac ttcaaaagag ttggaggagg
     7741 aattacatga ctgagaaaga acaagtacaa caaatcgtga agaagtataa taaaagtatc
     7801 g
//
LOCUS       DACEND010000116         7759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEND010000116 DACEND010000000
VERSION     DACEND010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.119"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            145..1092
                     /locus_tag="IW746_002173"
     CDS             145..1092
                     /locus_tag="IW746_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1079064.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1104..2024
                     /locus_tag="IW746_002174"
     CDS             1104..2024
                     /locus_tag="IW746_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1079065.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2065..3522
                     /locus_tag="IW746_002175"
     CDS             2065..3522
                     /locus_tag="IW746_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1079066.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3867..4481
                     /locus_tag="IW746_002176"
     CDS             3867..4481
                     /locus_tag="IW746_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1079067.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4474..6198
                     /locus_tag="IW746_002177"
     CDS             4474..6198
                     /locus_tag="IW746_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1079068.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6195..7646
                     /locus_tag="IW746_002178"
     CDS             6195..7646
                     /locus_tag="IW746_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1079069.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1289 c         1543 g         2309 t
ORIGIN      
        1 gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt
       61 tcattttccc tatactttgt ttatttattc tcattattga aagcgctata attacgttgc
      121 gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg
      181 aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt
      241 tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca
      301 gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa
      361 tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta
      421 gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg
      481 agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct
      541 tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat
      601 cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg
      661 ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac
      721 tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct
      781 agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt
      841 ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt
      901 cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat
      961 ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt
     1021 gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct
     1081 gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct
     1141 ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct
     1201 gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga
     1261 ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg
     1321 accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag
     1381 gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact
     1441 ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga
     1501 tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat
     1561 tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac
     1621 gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa
     1681 tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg
     1741 ccgcattggg ttactggaat gactggttca atgctttact atacatccaa gatgataagc
     1801 tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa
     1861 attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca
     1921 gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga
     1981 agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa
     2041 atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt
     2101 tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca
     2161 gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc
     2221 gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat
     2281 atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg
     2341 gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt
     2401 gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa
     2461 gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat
     2521 gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata cgatctttca
     2581 attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa
     2641 gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat
     2701 tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc
     2761 atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac
     2821 aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga
     2881 aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg
     2941 acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca
     3001 gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca
     3061 gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg
     3121 atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat
     3181 ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat
     3241 acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca
     3301 gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt
     3361 aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca
     3421 gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg
     3481 aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag
     3541 gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca
     3601 gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt
     3661 tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag
     3721 ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt
     3781 tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta
     3841 taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc
     3901 ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga
     3961 tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat
     4021 tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa
     4081 gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat
     4141 atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg
     4201 ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga
     4261 tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt
     4321 ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat
     4381 tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt
     4441 ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat tctattttca
     4501 aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg
     4561 atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa
     4621 aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg
     4681 agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc
     4741 tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc
     4801 gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta
     4861 agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac
     4921 aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg
     4981 cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa
     5041 ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct
     5101 ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa
     5161 agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat
     5221 ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct
     5281 gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt
     5341 ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat
     5401 tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag
     5461 acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac
     5521 gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag
     5581 caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca
     5641 ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg
     5701 tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa
     5761 gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga
     5821 gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg
     5881 atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat
     5941 gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat
     6001 ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa
     6061 gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc
     6121 ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt
     6181 acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt
     6241 tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag
     6301 cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa
     6361 cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac
     6421 gtcataattt tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag
     6481 gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg
     6541 aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag
     6601 aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg
     6661 aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg
     6721 caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact
     6781 atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg
     6841 atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg
     6901 gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc
     6961 tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag
     7021 aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat
     7081 tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga
     7141 tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga
     7201 ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg
     7261 tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt
     7321 acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta
     7381 cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca
     7441 aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga
     7501 aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca
     7561 acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt
     7621 accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa
     7681 aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc
     7741 atgacctctc gataaacgg
//
LOCUS       DACEND010000117         7643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEND010000117 DACEND010000000
VERSION     DACEND010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.120"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..394)
                     /locus_tag="IW746_002179"
     CDS             complement(44..394)
                     /locus_tag="IW746_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079070.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(387..1712)
                     /locus_tag="IW746_002180"
     CDS             complement(387..1712)
                     /locus_tag="IW746_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1079071.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(1881..2465)
                     /locus_tag="IW746_002181"
     CDS             complement(1881..2465)
                     /locus_tag="IW746_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079072.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(2676..2879)
                     /locus_tag="IW746_002182"
     CDS             complement(2676..2879)
                     /locus_tag="IW746_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079073.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(2889..3311)
                     /locus_tag="IW746_002183"
     CDS             complement(2889..3311)
                     /locus_tag="IW746_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079074.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            3625..4239
                     /locus_tag="IW746_002184"
     CDS             3625..4239
                     /locus_tag="IW746_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079075.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(4404..5408)
                     /locus_tag="IW746_002185"
     CDS             complement(4404..5408)
                     /locus_tag="IW746_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079076.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(5867..6124)
                     /locus_tag="IW746_002186"
     CDS             complement(5867..6124)
                     /locus_tag="IW746_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1079077.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(6117..6386)
                     /locus_tag="IW746_002187"
     CDS             complement(6117..6386)
                     /locus_tag="IW746_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1079078.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(6517..6696)
                     /locus_tag="IW746_002188"
     CDS             complement(6517..6696)
                     /locus_tag="IW746_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079079.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(7077..>7643)
                     /locus_tag="IW746_002189"
     CDS             complement(7077..>7643)
                     /locus_tag="IW746_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079080.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2340 a         1455 c         1106 g         2742 t
ORIGIN      
        1 tgatcaaagg ttatatactt tagctcatat tgctggataa tgttcattct aatcgactcc
       61 atttattatc ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt ttgtcttttc
      121 caacgtagat ccccagctca tcactaccta agatcttgcc aatgatatct tctggatagt
      181 atccttcagc gtttcgttct tctttttgaa tagatactga tctatttttt atgactgcag
      241 cgttcaatac ttccgcaatc tcttcagtcg tcatttgttc ttttgctaaa ttttgactgg
      301 ttcgttgttc attgtcggca tcaatttctt cagtatgatc agataaataa aaccctgccc
      361 actttaaacg tccacgatca ttgtaatcaa tcatttttaa ttcccccatt tccgcccgca
      421 tgaccaccaa caagtgtgct tctagtaatg cttctagcgc cttctaacat tgaagaagca
      481 tgtacaatcg acgtaaaacc atatttttgc cgaatcttgt caataatttt atctaaattt
      541 tcttcgtcaa tttgcttttg tggatcacta aataaatcca gctgaagcga atcggtgtac
      601 acaagcttgc tataggttat tccaacatgc cttacttctt gtccttcata atacttatca
      661 aacaagaaaa gacaataatt ctgcaattcc tttgtattat ctgtgatagg gatcttcatc
      721 tggtgtgaaa accctggtct cgtttctaag atcgacgtgc caatgtgtaa atggacacaa
      781 cctgttttac agttatgctg cctaatgcgt atcgctactt gttccgccat ttcttttacc
      841 accagctcga tttcatttct tctagtataa tctcgtggta acacttgtga atttccatat
      901 gatttttcct ttgtcggttc tggtgggatt gctatatctg ttcgatcgat cccattagca
      961 tggaagtaca gctgtaatcc aatcacacct aatctattct taatcgtata aggattccag
     1021 ttagcaaggt ctctaatgct caaaattccc atttggttga gccttttttt cattcgcgaa
     1081 ccaattcccc aaaagtccgt catttcggga atattccata ctttctcagg aacgttttcg
     1141 tacgtccatt ccgcaataaa tccttcatta tgctttgctt cattgtctaa ggctaattta
     1201 gccaaaaggg gattatcgcc gacgcctacc gtagcaatca aacctaactc ttctttaata
     1261 cgttcttgga tcatttgagc gagcttcttc cttcgctgac ttcgtgtttc ttcagtcgta
     1321 aaaagattca gtgattttgt cacttttaaa atcgattcat cgatcgagta aatcagtaga
     1381 tcttcatcag ccacatatct tctgaaaata ttatttacct gcatatttcg cttgatatat
     1441 aggttcatac gtggtggaac aacgtgtagt gttttaggaa atggttgtgg taagtcacgt
     1501 ggtctactca catttgtaat accataccgc ttttttgctt caggagaaga agctaatatc
     1561 aatcctgaac cagtattatc agatcgactc ataacaacaa gttctgttgt taatggatct
     1621 aaatttcttt ctatacattc gacacttgcg taaaagcttt tcatatcgat taaaaagtaa
     1681 tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt ccacattata
     1741 caaacatatg ttcgatgttg taaagaagaa atagctaaat atttgagaat aagttatcag
     1801 tagacactta caaaaaattc tcatactatt gtcacttttc tagataaatg gcaaaaattt
     1861 tcagcattaa atgacaaaaa ctaatgagaa tttgtctttc catttttctc ctccattttt
     1921 gccaaataac gataaatggt ggttcttgag acgttccacc gttccgcaat agtcttaata
     1981 ggtgtaccgc tatccactaa agtcttcagt aattccaaat cttgttcaga caatttttca
     2041 ggacgaccac caaaccgtcc tcttgcacga gcagctgctc gtcctgctgc ggatcgttcc
     2101 aggatcaaat tacgctcaaa ttctgcaaat gccgcaaaca aatgaaacat gagctgtcct
     2161 gtagaacttg atttgtccat cgtaatattt tcttgaatac tatgaaaact cacaccacgc
     2221 tcattcagtc gattgacgat tgaaatcaaa tcttccatat ttctacctaa acgatctaag
     2281 cgccaaacga caagtgtatc gccagagcga acaaaatcaa tggccatttc taatcctggt
     2341 cgattacttt ttgccccact catatgatca gtaaaaattt tttcacaacc aaatttattt
     2401 aggctgtctt cttgtaaatc caaattttgt aaaccagtag atacccgggc gtatcctatt
     2461 ttcaaaaaaa tcactccttc attaatttta ttgtaacata actttatcca tatagtttaa
     2521 ctgaacatag atattgttat gggtttatga acatgaaatg aattgttttt ttgaaaaata
     2581 aagcaaaaaa agaaagtgtc cagaaatggg tgattttatg aacactttca attagaaaaa
     2641 aagaatgtcg actagacact ctttttttaa aatcattatt tttctttttg atatgttttt
     2701 tggtctcttt tttttctttt ttcttcagaa tcttctagat tagaaattga tttttgaatt
     2761 ttttgtgtct cctgtaaagt tcgctgaact tgtaaagctg attcttcttt cggtaaatta
     2821 attttactta gaatatcgct atcttgataa aaatttggtg tgttcacaaa ttctggcata
     2881 aaaaatccct actttctagt taaggttcct gaataagtcg caatccaatt gtttccagct
     2941 gatttataac tttgtaaata caattttcca tagtaccaat aaccatttct tttttctttc
     3001 caattccgtg attgaggagg tctagaaggc caaccaactt tatcaaaaat catcgttcct
     3061 gtcactcgta tcgttttaga agctgctcga gcaagtaatg gagaaactat taaataattt
     3121 tcagtaacag ttaagtcgtt agttgggtta ccaatggcta ttccgttcct atctaaagga
     3181 gttaattctt ggttattatc tactacagta gttggttcat ttgtactatc agcaaatgta
     3241 ggaattgtag ttccagttac actaattcca atagttaaaa tacctaaaga taaaataaac
     3301 ttatttttca ttgtctatca cctcgaaatc aaaaataaga aaacgttttc ttaataaatc
     3361 ttaacagata aatcaagtta tatactagaa aattcataaa atatattttt aatgtcataa
     3421 tcgtttaaat tttcttgatt gacatttatt tacaaaaaat attgccattt aaaaagaaaa
     3481 gtgacacata ctatttcctc catcattagt ttttgtctat ataaggttta ccctaaatag
     3541 acactggtat aacattgaaa aatatggctt cctcgtgtat gattagggtg tattttaaat
     3601 agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc atcgatcgat
     3661 caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag aattttcatc
     3721 gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc attagattac
     3781 gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag aaattatgac
     3841 gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat cattactagt
     3901 ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat taaggatctg
     3961 atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag
     4021 gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc caaattatac
     4081 agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt
     4141 aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag acaaaccgtg
     4201 tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct gcttttatta
     4261 tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt cttactcaga
     4321 acagataaag cataaaattc caacaaacca ttttttactt aactaaataa agagtctgtt
     4381 aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc cttatctata
     4441 ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt gtatgtcgac
     4501 acattggtta tactaattcc tatagctagt tcttgagact ctacttttct ctctagctcc
     4561 tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat agattcttca
     4621 taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa tgtttttagc
     4681 actgccatat aacattcaaa acattcttct atagttggtt tacttccttg aggagaatga
     4741 accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc tttcggaaac
     4801 tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg gggagattgt
     4861 aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc ctttacccct
     4921 atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat atttttactt
     4981 cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta attaactaat
     5041 tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg aagtgtcttt
     5101 aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct cgaagtataa
     5161 tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc ttttaaacgt
     5221 tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata aaaaaaaaca
     5281 tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc attatcttga
     5341 ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta
     5401 tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg
     5461 taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct
     5521 aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta
     5581 ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt
     5641 ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct
     5701 caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat
     5761 taataacaat caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag
     5821 gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt
     5881 ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg
     5941 taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc
     6001 cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt
     6061 tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa
     6121 tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca
     6181 tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc
     6241 atagaatcat aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt
     6301 acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg
     6361 aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat
     6421 tgtacattat attgtacaat taagcaaatg attaatattc taacttctta taattaaaag
     6481 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
     6541 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
     6601 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
     6661 aacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
     6721 taaccctatg aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac
     6781 aaagcgccaa aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg
     6841 cacagctgca gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat
     6901 gattttattc atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt
     6961 cgcaccacct aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct
     7021 tagttactag gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat
     7081 tgcctttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
     7141 atttcttctg tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgtgtaccag
     7201 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagcg
     7261 agttcataat ccattcccat taacacgata gattccgttc cacacgtcaa aaatctagta
     7321 aatacaaggt aataatcgtc tatcgtcgca tctttgctgt ttagctcttc catttcttct
     7381 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
     7441 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     7501 atgatgtatt tttctgtttc tagtcgtaga agctccgttc ctttttttac cgtttcagga
     7561 cgacgtttat acacgtctaa acgattatgg ctgactgtga ccttcgtttg actcgtttca
     7621 ctttccaaat acaattgttc ttc
//
LOCUS       DACEND010000118         7613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEND010000118 DACEND010000000
VERSION     DACEND010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.121"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(123..566)
                     /locus_tag="IW746_002190"
     CDS             complement(123..566)
                     /locus_tag="IW746_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079081.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(654..1019)
                     /locus_tag="IW746_002191"
     CDS             complement(654..1019)
                     /locus_tag="IW746_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ1079082.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(1363..3015)
                     /locus_tag="IW746_002192"
     CDS             complement(1363..3015)
                     /locus_tag="IW746_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1079083.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3075..3654)
                     /locus_tag="IW746_002193"
                     /pseudo
     CDS             complement(3075..3654)
                     /locus_tag="IW746_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(4886..5602)
                     /locus_tag="IW746_002194"
     CDS             complement(4886..5602)
                     /locus_tag="IW746_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079084.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5627..6025)
                     /locus_tag="IW746_002195"
     CDS             complement(5627..6025)
                     /locus_tag="IW746_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1079085.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6113..>7613)
                     /locus_tag="IW746_002196"
     CDS             complement(6113..>7613)
                     /locus_tag="IW746_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1079086.1"
                     /translation="IDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSL
                     IGCDEPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNEL
                     KEDDGKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIK
                     TNNWFDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLP
                     AQVEYVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCS
                     DFKRYSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQI
                     EALLSVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYR
                     YELAKLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEI
                     GSDLKKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2555 a         1631 c         1115 g         2312 t
ORIGIN      
        1 ttttatctcc tttttaccaa ccatctacca ctaggttacg aactacctaa gtgcccacca
       61 tccgccgcgt ggaggctgtt aatcaggatg gcaaccgccc ggtagaggcg acaattatgc
      121 tatcatttta atttaagtct atataacata atactataat aatatgacaa caccaacgtt
      181 ataggaatgg aaacaaatgc cgccaaaacc aagttaggga taatttgatg aatcgtacta
      241 aatacaagaa tatggagtag atatatatca tatgagtatc ttgaaatcct aggcattatc
      301 tttctaaatg gaataacttg atacattaaa attgcaattg caccgtaacc gaagaatgca
      361 aaaatatcat taatagactc catcagtcca ccaataattt tcgatatacc agcaaaacca
      421 attccaacaa tggacgccat aatcacgaat atattattaa atcttactat ttttcttttc
      481 actaaatact ctgcaaccaa acatgccgaa agtaaattcc caaatatatt gcgcaaagca
      541 gctcccaaga actcgtatgc ttccatatcc tgtatgaaac atcataatag cccagatact
      601 aataactcag cttgcaacaa gcaatcattt cattcctatt tttctctata gttttacaat
      661 tggtatgaat aagaaataga actgaataat tgtcgaaata aaccaaaggg acccagtgac
      721 tcctcatata ctggtagaaa aattttaaat aacaaaacat gagacaaata tacttttgca
      781 gcatttaaat cttgtgtcat ataaggtagt aacactgata ccgtaattac gataatatac
      841 ggaatataaa tcttgataaa tcgtttctgt aaaaattcac ccaatgtaat aggctttttt
      901 aaatatgaca gatatagccc aaaacctgag caaaagaaga atatatgcac tcctgtatca
      961 ccaatagata ttgctttgtt cagtattgct ggcaaatacg attgctgaaa atagtacatc
     1021 aatacaatag cgaatattga aaacgccttc tcaaaactaa taatatcacg tttatttctt
     1081 tccccctcat tttattattt caatagtttt attcatattc tactcctttc atctctattg
     1141 ctgaatacat tattaaaact ctaatatctc ttatttctcc cttactgctt tgatcggtgc
     1201 ttcatcaggc tgctgtgtct ctgttaacac ttctttgcct tttcgatcca cacagtcggc
     1261 ccctttacct caattatgta aacagaaccg taaagcagga aatttttatt aaacatagat
     1321 gttgctactt cggacattga cactcagatc tttttcatag tattacttaa acatttcagc
     1381 cagttcagca gccataacag cttccattac ttcggcagct tccttgcttt ctgcgctaac
     1441 gctgatatag gtcttcagct tcggctcagt gccggaaggc cgaaccacta cagagcagtt
     1501 gccctccagc agatatttga gaacatcaga cttcggcagg ccattcagtc ccgtgctgta
     1561 atccaaggtt tcaatgacct tcttacctcc aatcatatca agacctttat ggaattcaac
     1621 cataattttc tgcatcttgg taaaacctgc cgcaccctca aactcaaagg aatgcagggt
     1681 gttcaaacag tagccatact tagcataaat ctcgttcagc ttatcaaaca gactgatacc
     1741 ctgagtcttg tagtaagaga acatctcgca gatcagataa gcggcgttga caccatcctt
     1801 gtcacggaca tagctgccgg atagatagcc gtaggattcc tcaaagccga aaatatagct
     1861 gtccaccttg cctgcctttt ccagcatacc aatctgttcg ccgatgaact tgaagccggt
     1921 caacacatcg acagtacgca cgccatagtt ggaagcgatc cgctcgccca gatccatagt
     1981 cacgatggtc ttgaccatca cagggtcagt gggcatcttg ccgtgcttaa ttctctgaga
     2041 gcatatgtag tccagaagca acagaccagt ttcgttaccg gacagcagga cataatcccc
     2101 ttctgcattc ttcacggcga tacccacacg gtcgcagtca gggtcggtag ccagcagcag
     2161 atcagcatta tacttcttgg catactccat gcccagagac atagcttcat taatttccgg
     2221 attcggataa ggacaggtcg ggaaattacc gtctggcaga cgctgctctt cgacaacggt
     2281 cacattgctg tagcccattt ctgccagagt acgggtaaca ggcaccagac cggtaccatt
     2341 caaaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca gaaccgactg
     2401 gccctttact tcttcaatga aagcagtcag aacctctggc tggatagaag cgattctgga
     2461 agcgtcgccg cccattttca catctgcaaa aatatccagc ttttcaattt cagccagaat
     2521 ctcagcggca gcctcggtgg tgatctggca gccgcctgcg ccatagacct tatatccgtt
     2581 atacttggaa ggattatggg atgcggtgat cataacaccg gcagaagcct tcagataacg
     2641 gatagcaaag gatacggtag gcacaggcag cagctccggc cacagatgca cctgaatacc
     2701 gtttgcagca aagatgtccg cggcaacctt tgcaaataca tcggacttga ttcgggaatc
     2761 atagcccacg acgacagaag gggtttcata ggtcttattc agatagtctg acagtccctg
     2821 gcttgcctta cctacggtgt gtacgttcat acgattagtg cctgcaccaa tggtgccacg
     2881 cagaccaccg gtaccaaagg acagattttt atagaaggag tcctcaatgt ccttctcgga
     2941 atatgaccgc agttcctcta caatttctgc attggcattt gtaagccaat gataataatt
     3001 tgcattatga tccatagaat ctctccaatc ttttaaaatt ctgctcattt atttttaagg
     3061 ttaggatgaa tatatcagct caaataatat ataaccccac taatgccgcc acccaaagta
     3121 ttataaaata aatctgacaa ttggaatgtc tctatgtgta aaaataactg caaaaactct
     3181 ataagacacg aacataaaaa aacaaatttt ataatctctg ataaattttt ccaaaatctt
     3241 tttctgaagc cttataccac attactagaa ttataaatgg aataaacaga ataacatttt
     3301 caaaacattc tgtagttaat ctaatctccc ctgtcgtttc attcacagag taaatccacc
     3361 aactaccaaa cacattagac aaaggattta gccaaacata tctatccatc agtgttctaa
     3421 aaagaatcat atttgtaaaa aacacaagca aaaacatttt tctaaatgag cttgattttc
     3481 tgaaataccg aatccagaca agaaaagatt ttttcaaacc taatcctgat ttctcctcag
     3541 ccatcgcaaa caagtataaa aacatagcca aaactgcaat taaaatcgaa acccaaatat
     3601 ttgatacaat gcagataaca cattaaccaa aatagaagat atgattttct tcataaactc
     3661 tcctttcatc atgcgctaaa tgagtaccat caagaaggtt tcgtaaaagg aggtacccac
     3721 tctaacatga ataacctcgg ccagcgaaaa taaaactcaa cagaattcct atctctatgc
     3781 tgctataatt tcaacaaatt tacatgtacc agttactaat ctaatataat tttttcagca
     3841 aggtaaattt cacactacac gcatacacaa aaactgtaga aattaaaatt attatcattc
     3901 cacaagtcat ttctattaat gaatttgtaa tccccaattt tgggaaaata aaatttaaag
     3961 cagctactat atacggatgt aaacaaaata ataacgtact aattcttcgc agatgtactg
     4021 ttatttttat aaatgtacta tctttaggct cagtgatact gatacaacta ccagtgctaa
     4081 tggaatggaa aatattaaca aattattgac ttctcgcaaa gctatttgat ttatgatgaa
     4141 actttcacca actaataagc atcctatagc taaaacagca cataagataa aattacggta
     4201 tttaaagacc ttattaagta tagtgttttt gtggaaggct aagtgttgcc caataatgac
     4261 gcatggtaca cctataaata atccattacg agttgttaca aacacgcttt tataacaata
     4321 tctaatatac tcgatggaat cattcccaga taatataaac caataagatt caaatgctaa
     4381 acctactacg catagcaaca aaccactgag aaactttatt ttagaaacct ggcttttgcg
     4441 tactaaccat agagcaaaag ccagtgcagg caagaaccac aggtgatagt aactaccaat
     4501 aaacagaaaa tctctgatat acaataatac atctgttttc cagctaaaat ctgactgtac
     4561 ccaattcttt aatactaatg gaatatataa tatagaccaa ataatgtaaa ttttaagtac
     4621 tctgtagatt tgtttagata atttagcgga tgtaacttca ccactacaca acaaaaagga
     4681 actgatgatg aaaaaagtag gaacggcaac cgtactcagt cctgcaacaa taccaatact
     4741 aaatgcttaa tctggagcta aatagttttg acctaaatga cgaacaacaa ctaaaatagc
     4801 cattagtatt tttagaatat ctatcgaata gtactccccc acacacgcag ctttataatt
     4861 aacattttct atgattatat tttccctatt catttaagaa cacatcgagc tcttctggag
     4921 taccgagtac gtgaactttt gatttatcta ctatactaat ggttaccttc atgcccttct
     4981 ctatcatata attatatagc ggcgcaatat atttctcatt cttttccaga tgcctttcat
     5041 ctgcataata ttcttcgtag agtgatgcat ataattttgc agaagaaaaa taatatgcac
     5101 ctaaagtaca attgttagaa attctaactt tttctcttac ttctactaca tcaccatatt
     5161 cgttcaactt ggcaaaactc caatgatcac cgtccgcatg gaaacacgga atatgtcctt
     5221 ctccagaaat atcaacgtat ttcatctcac ctggctcaac atatgtatct atgttataaa
     5281 ccataattgc attatcaggt gcgcaataag gaattgcaag catacatgta gtggcttgtc
     5341 catcagtcat atagtcaatt tcaactacct cgacattttc aattccatac agactgcaat
     5401 gttctttgat aaaaacagta gcatcatctt ccttacgcac aacaaaaaca tacttttcta
     5461 catgtgaatt ataatcaagc aagctgtcta aggaccaatc aaataaggtt ttaccttttg
     5521 cttcaatcat aaatttgggc acagtgtagc caactttacg aaagcgactg ccaagaccag
     5581 ccattgtaat aataaccgtc atcttattca ctttataaag tctcctttac tcagatttca
     5641 atttggattt tttgcaaatc tcatcgagct gatccagggt acacgataaa aattcatccg
     5701 gacgaactgt gcgatcatca acataaaacc ctttatggcc tggccaaggc tttccataaa
     5761 gaatttcatc atacggaatc tcccacttat ctagccaagc caacaagatt tttgcagtat
     5821 ttgcattgat cacacctaaa ttaccatgat aagaattcat attgcgagat gtgaacagtg
     5881 taatctttgc tccgttctcc ttatagtatc gcaacttctc caccatatta gcatacggaa
     5941 ctaaatcttc atatttctca tctttctttt tgatggggca caaagttcca tcaatatcaa
     6001 aaacaaatgc gtaatcttta aacatataac taagacatct cctttatttt agccaggcaa
     6061 tttatatgcc cggctttaca ctgatcaaat agaaaaacaa tagctcaaag cattaacaat
     6121 aaatatccgg gacaactcgt cctaaaattt ccaaaccagt tgctaacata gccatctgat
     6181 ggtttaagct ttctccatgc aaaggaatca tacttaaaaa cagcaaagcc tcaataagtt
     6241 caattttttt caaatcagaa cctatttcat ctttgaagac agagtaaaat acttcatata
     6301 aatcataatc tcgtttacga tcttgaacaa tatagtctat gattgccttc tttggatcat
     6361 attttattgt aaacaagtcc ttaattataa agtcatattt tccatcaaca ctatgaaaca
     6421 attttgccag ttcatatcta taatcaccat aaatatcaaa atctccaaac ttacctcgcg
     6481 gatctatgac tttaatgaac gaaaatgtgt tatctaccat aatattggca aagcataagt
     6541 caccatgaat aatattaaat tgcgtaatgt caaacaattc tcttggaaca cttacgctca
     6601 ataaagcttc tatctggtcc aaagatttat atctgactcc atttatttga atatcagaag
     6661 aaaagaattc tgtgaaccgg gggtcctttc tgagcttatt gaatctctga aatgttttat
     6721 cgagatacat atctttcaat gacttttgaa tattatcccc tgaaacagag tatctcttaa
     6781 agtctgagca tacaaatcgt attctattaa agatatctat ccattgtttt tttgttaagt
     6841 cactatagag aaataactcg tgaacagtat gatacgaata gtattccata cttacatatg
     6901 gattaatata cgaagtagaa tattcgaata ttctaggtct cacatattct acctgtgctg
     6961 gcaacttaag ataccactta atttctccga taaatttttc aacatcttca ctaatttttc
     7021 tcaggatgct acgatcttta tcaattgaaa tgtggttaaa ttctcttgcc ttaacttcca
     7081 atttggaatt ataatattta tctgcatgtc caatgtcaaa ccaattattt gttttaatag
     7141 agagaaattc atatcgtttg ctatactctt gtaaagcttg ataaaaacta ttcacattta
     7201 ctacatttga catcaaagct ttacgcaaac actctctaaa atactgggca tccataattt
     7261 gaaatacacc agaaaacagt ttgtgcttct ttccatcatc ttcttttaac tcgttcttat
     7321 ctaaaacaga aataatatct ccgtcttttt cttcaaagaa tgtccaagta ttactataat
     7381 agtcttcagc atagaaaaag ctgtcacatt ccaaggaata tatattatca ttgataatgg
     7441 tatcagcaaa attaataata acaggctcgt cacatcctat tagagaatca tatatagttc
     7501 ttccaagatc ccctaactct ttaagctgaa taatatttac tgttttagat ttaatatact
     7561 tatctaatcg tcttgctacc ttatccatct tttcgtaaca ggcaatatcg ata
//
LOCUS       DACEND010000119         7555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEND010000119 DACEND010000000
VERSION     DACEND010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.122"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(102..626)
                     /locus_tag="IW746_002197"
     CDS             complement(102..626)
                     /locus_tag="IW746_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079087.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IW746_002198"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IW746_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1079088.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IW746_002199"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IW746_002199"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1079089.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="IW746_002200"
     CDS             complement(2348..3028)
                     /locus_tag="IW746_002200"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1079090.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="IW746_002201"
     CDS             3154..4023
                     /locus_tag="IW746_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1079091.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="IW746_002202"
     CDS             4049..4564
                     /locus_tag="IW746_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079092.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="IW746_002203"
     CDS             complement(4611..5174)
                     /locus_tag="IW746_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079093.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="IW746_002204"
     CDS             complement(5229..5408)
                     /locus_tag="IW746_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079094.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="IW746_002205"
     CDS             complement(5485..5994)
                     /locus_tag="IW746_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079095.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="IW746_002206"
     CDS             complement(6090..6470)
                     /locus_tag="IW746_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1079096.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="IW746_002207"
     CDS             6800..7549
                     /locus_tag="IW746_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1079097.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2144 a         1567 c         1339 g         2505 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaac
//
LOCUS       DACEND010000120         7510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEND010000120 DACEND010000000
VERSION     DACEND010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.123"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..2166)
                     /locus_tag="IW746_002208"
     CDS             complement(1..2166)
                     /locus_tag="IW746_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1079098.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2426..4720
                     /locus_tag="IW746_002209"
     CDS             2426..4720
                     /locus_tag="IW746_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1079099.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4713..5378
                     /gene="pgmB"
                     /locus_tag="IW746_002210"
     CDS             4713..5378
                     /gene="pgmB"
                     /locus_tag="IW746_002210"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1079100.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5371..6378
                     /locus_tag="IW746_002211"
     CDS             5371..6378
                     /locus_tag="IW746_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1079101.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6400..7419
                     /locus_tag="IW746_002212"
     CDS             6400..7419
                     /locus_tag="IW746_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1079102.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2342 a         1638 c         1409 g         2121 t
ORIGIN      
        1 ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt gcttgatcac
       61 tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt tttcttcgtt
      121 ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt cttggccttc
      181 tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc ccatatggat
      241 caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag ggaaaatact
      301 tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa cagcaaagcc
      361 gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag gaatcacttc
      421 tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac tacctgcttg
      481 tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt gcagcagatc
      541 ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga ctccattgtc
      601 tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc ctactttttc
      661 tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt tgatattttc
      721 ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc ctgttccgct
      781 gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc ccggtgtagc
      841 aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac caaaagcgat
      901 cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag ctaacggtgt
      961 ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga tatctgccat
     1021 cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag cagcaaacat
     1081 aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg ctgctgaaaa
     1141 atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca tcgcaaaagc
     1201 cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac gagccggcgt
     1261 ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa gattcactaa
     1321 atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc ccgcctgtgc
     1381 gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa tcgtcagcat
     1441 atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa acggtgctag
     1501 aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga ttccagcctg
     1561 tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata agataacaac
     1621 aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt ttctataatt
     1681 atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta cgaatactcc
     1741 catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca tgatcttcgt
     1801 accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg tgacaccaaa
     1861 gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa atgctccgcc
     1921 tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat gaagattacc
     1981 aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg gcgtccaatc
     2041 aggattgatc aagggaattg atttccctat actgatcatc aacccagctg ccggcataac
     2101 tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac taaatatctt
     2161 tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat agcgtgcgtt
     2221 ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta taaaacctat
     2281 aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa gcttttttct
     2341 atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt atgaacgtat
     2401 aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg ctattgatcc
     2461 atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag aatctttaac
     2521 aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt actcagggga
     2581 ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac gtgtcggctg
     2641 gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga actttatcgc
     2701 gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac cagaagactt
     2761 ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa tcactacttc
     2821 tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa aagaatcagc
     2881 ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac tcgtatcaaa
     2941 attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt ggaatgagat
     3001 tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc caaacaactt
     3061 tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg gaaaatcagt
     3121 catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg cagaattatc
     3181 tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta gagatattcc
     3241 agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa acaattatat
     3301 ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg ccgatgtggt
     3361 catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc aactattctc
     3421 tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca caggagaaaa
     3481 atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt atcttgcttt
     3541 agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac tgccgcaagc
     3601 aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg tcaccttcac
     3661 tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc gaaatggcgc
     3721 gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat atttgaaaaa
     3781 tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg tccatttttc
     3841 aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat atgagaacaa
     3901 tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt acacgcgaga
     3961 aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag aactagccaa
     4021 atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag gaatctttgt
     4081 ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc ccacaagcga
     4141 tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct tcatcaagca
     4201 ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag aagaaaagca
     4261 aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt cgccaagtat
     4321 ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa tgtaccagcg
     4381 aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct tgcacatcac
     4441 ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga aaactgccca
     4501 tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt ctttccatat
     4561 caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta ctttcaagtt
     4621 attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt tagcggatcg
     4681 tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg tttgatttag
     4741 acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag cttggtcagg
     4801 aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc agccgagaag
     4861 actcactctc tttgctttta gcttatggcg gaaaagaaca ctcttttaca aaggaagaat
     4921 ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag acgatcgaac
     4981 caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa gaaaaaatca
     5041 agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa atgggtctta
     5101 caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa cctgcgccag
     5161 atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt atcgggatcg
     5221 aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca attggcgtgg
     5281 gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact tttcttacga
     5341 tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc cgtcgtacca
     5401 gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga agtcgttctt
     5461 ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg aaaatcagaa
     5521 aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag cttttttgga
     5581 gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga gcatagattg
     5641 gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc tttccaattt
     5701 tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta cttaaaagat
     5761 gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt aacaaaaaac
     5821 gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa tccgaccaac
     5881 catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca tcagttaacg
     5941 gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg aaattggaca
     6001 aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca
     6061 tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact
     6121 tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa
     6181 agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag
     6241 atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc
     6301 cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat
     6361 cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat
     6421 gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct
     6481 agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt
     6541 cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta
     6601 ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca
     6661 atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt
     6721 gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt
     6781 ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa ccacatccct
     6841 ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat
     6901 aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc
     6961 ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa
     7021 aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag
     7081 gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt
     7141 gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta tgtcattcca
     7201 gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac
     7261 cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc
     7321 gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca tcgcttgatc
     7381 aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg
     7441 ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt
     7501 gtttatctgt
//
LOCUS       DACEND010000121         7431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEND010000121 DACEND010000000
VERSION     DACEND010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.124"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW746_002213"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW746_002213"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1079103.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3170)
                     /locus_tag="IW746_002214"
     CDS             complement(1929..3170)
                     /locus_tag="IW746_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1079104.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="IW746_002215"
     CDS             complement(3188..4111)
                     /locus_tag="IW746_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1079105.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="IW746_002216"
     CDS             complement(4332..6824)
                     /locus_tag="IW746_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1079106.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="IW746_002217"
     CDS             complement(6831..7298)
                     /locus_tag="IW746_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1079107.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DACEND010000122         7396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEND010000122 DACEND010000000
VERSION     DACEND010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.125"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..3759
                     /gene="nifJ"
                     /locus_tag="IW746_002218"
     CDS             67..3759
                     /gene="nifJ"
                     /locus_tag="IW746_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1079108.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            3948..4835
                     /locus_tag="IW746_002219"
     CDS             3948..4835
                     /locus_tag="IW746_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1079109.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4832..6001
                     /locus_tag="IW746_002220"
     CDS             4832..6001
                     /locus_tag="IW746_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079110.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6025..7380
                     /locus_tag="IW746_002221"
     CDS             6025..7380
                     /locus_tag="IW746_002221"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1079111.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2409 a         1363 c         1718 g         1906 t
ORIGIN      
        1 aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga
       61 tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat
      121 gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta
      181 gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt aaaagtagta
      241 gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca
      301 ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa tatgtacaag
      361 attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt gacgacaaat
      421 gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct
      481 atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct
      541 tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac gagtcacgag
      601 attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaagagaaa
      661 ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac
      721 cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta
      781 cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac
      841 ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt
      901 gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt
      961 ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat
     1021 acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct
     1081 ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt
     1141 ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat
     1201 gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat
     1261 gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc
     1321 caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc
     1381 aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa
     1441 aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg
     1501 tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat
     1561 gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac
     1621 gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt
     1681 agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc
     1741 aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg
     1801 cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta
     1861 gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca
     1921 gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata
     1981 caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt
     2041 gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg
     2101 ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt
     2161 gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa
     2221 gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt
     2281 atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca
     2341 aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg
     2401 gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg
     2461 tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa
     2521 acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg
     2581 acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa
     2641 tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat
     2701 ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta
     2761 ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt acacatgttt
     2821 gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga
     2881 tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc
     2941 caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat
     3001 gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt ctattccaat
     3061 actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt
     3121 ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat
     3181 gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt tgaagaagca
     3241 gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac acatggcttg
     3301 cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggttattgg
     3361 tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat gactttggac
     3421 tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc
     3481 tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tgtccacgac
     3541 gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa
     3601 cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg
     3661 gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga acgacgtaaa
     3721 aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata taatgaataa
     3781 ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact tgcaattttc
     3841 ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta aaattaaaac
     3901 atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg tcatttcaaa
     3961 tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac ttgttctctc
     4021 tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta tacaaactga
     4081 tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct atttttattg
     4141 ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg atgaaccaag
     4201 tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc cggagaggac
     4261 tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag caggaagatg
     4321 agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga aaaatcggtg
     4381 cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca gggattgctt
     4441 tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat acaccgctgc
     4501 atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc tatcttcctc
     4561 tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca gctgtcggta
     4621 taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg gacgtcagca
     4681 tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg gcaatcttgc
     4741 gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt caatctttcc
     4801 tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac ggacaagcat
     4861 tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg caaatggcca
     4921 gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta cgggggtgcc
     4981 gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa atgcagtgac
     5041 tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg aagatacacg
     5101 gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg aagttccgct
     5161 aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga ctattacagt
     5221 aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc cttcaacgtt
     5281 cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag tctctatcac
     5341 tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg acgcttcgat
     5401 ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg cagcagggga
     5461 agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt cgatcaagcc
     5521 aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg gggtagaaag
     5581 ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg atttgcttgc
     5641 atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc atcgaacaac
     5701 tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat cagttagtgt
     5761 ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga gcagtcggac
     5821 acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa cggaaccatc
     5881 gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca caagtacaga
     5941 agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct cagaaaatta
     6001 agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg cgtacgcggt
     6061 gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg cggaggttat
     6121 gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg gcgggataca
     6181 agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc ggtgggtatc
     6241 gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac acgtttgcaa
     6301 aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga taatggaatc
     6361 aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga aattgaagca
     6421 ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt gggaactgta
     6481 gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac gatcaacggt
     6541 gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc aacaagtgtt
     6601 aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa tccagacggg
     6661 ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga gatggtcgtt
     6721 gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat tattgcggtg
     6781 gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc taaatacttg
     6841 gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag taacttagga
     6901 ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt tggtgatcgt
     6961 tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca gtctgggcac
     7021 atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat tcagttactg
     7081 aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt aacaatctat
     7141 ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga tgttccggca
     7201 atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag aatcctagtt
     7261 cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac tgatgagaaa
     7321 gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg actggattaa
     7381 gagatatttt cataaa
//
LOCUS       DACEND010000123         7385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEND010000123 DACEND010000000
VERSION     DACEND010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.126"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2031
                     /locus_tag="IW746_002222"
     CDS             <1..2031
                     /locus_tag="IW746_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1079112.1"
                     /translation="SNIASRILPMIPAPLIQIFLGVVLGLTEWGQSIDFEPELFLVMI
                     IAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGATLSFLLPSVPLAACFAF
                     GAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVTAFQFALGALITGSFSA
                     VNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTGYLLIELLLPFLAYVLA
                     EAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTTIAFTLNALVFIFLGIE
                     LTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFYVFKDGSKKFKKQLNEI
                     LILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGVILVTLVIGIIVLPMLT
                     ETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEAVIENYQERIRDLYLED
                     LTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYSRFLSRFEHSITSQILS
                     FIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEIRETYQKNTQLIIESLD
                     NLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKKMLRGYYLERKIID
                     EYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFRAKSKKTSPIK"
     gene            2191..4077
                     /locus_tag="IW746_002223"
     CDS             2191..4077
                     /locus_tag="IW746_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1079113.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4089..5036
                     /locus_tag="IW746_002224"
     CDS             4089..5036
                     /locus_tag="IW746_002224"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1079114.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5056..5583
                     /locus_tag="IW746_002225"
     CDS             5056..5583
                     /locus_tag="IW746_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1079115.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5646..6299)
                     /locus_tag="IW746_002226"
     CDS             complement(5646..6299)
                     /locus_tag="IW746_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1079116.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6431..7273
                     /locus_tag="IW746_002227"
     CDS             6431..7273
                     /locus_tag="IW746_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1079117.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         2503 a         1263 c         1566 g         2053 t
ORIGIN      
        1 tcgaacatag ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt
       61 ggagtggtat taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc
      121 ttagttatga ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg
      181 attctcaaaa atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt
      241 gtcggggtag gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt
      301 gcttttggag cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga
      361 gtgaatatcc ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca
      421 tctggtgtta ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca
      481 gtaaacgcgg ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg
      541 cttgtctggt tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc
      601 acagggtatc tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca
      661 tttgatgtat ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt
      721 aggaaaatct ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact
      781 attgctttta cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg
      841 ttctcgccag tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta
      901 ttaatctctg tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc
      961 aaagatggca gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc
     1021 ggtgtaaagg gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat
     1081 atgatgtttt accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact
     1141 ttggtcatag ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg
     1201 gatttgaatg ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag
     1261 gaaattgatc gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa
     1321 gaacggatac gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa
     1381 gaaatacaag cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg
     1441 ggcaagcttt cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat
     1501 tctatcacaa gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta
     1561 atgcgaatcg ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca
     1621 tttattagtg atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg
     1681 attattgaaa gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt
     1741 atccagcaac gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg
     1801 atgatccagc aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga
     1861 aaaatcattg atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat
     1921 cgcaggaata tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct
     1981 ttccgatttg cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa
     2041 aattttcaag ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct
     2101 ttttttgaga acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt
     2161 atgatacaat atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc
     2221 aaaaaaactt atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa
     2281 gaccgtatcg ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt
     2341 gccggtatcg atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt
     2401 attggttatc tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc
     2461 tttcaaggaa acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa
     2521 ctaggggaaa acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa
     2581 atgaataaag aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta
     2641 ggaatcagtg aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt
     2701 agtctggcgc aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac
     2761 catttagatt atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca
     2821 ctattgatgg ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa
     2881 ctttcatttg gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa
     2941 gcagaacgtg atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag
     3001 gaattagctt ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc
     3061 aaccgtttcg aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag
     3121 ctgaacctag cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac
     3181 cgtataaatg accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga
     3241 ctaggtatta ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc
     3301 agaattcccc tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat
     3361 acacaagaaa atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct
     3421 gctgaagaag caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga
     3481 tttttatttc caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa
     3541 cgccggttat ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa
     3601 ccgaccaatg atttggacat agcaacattg acgattttgg aagattattt tagaagtttt
     3661 ccaggtgctg tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag
     3721 ttattagtat tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat
     3781 ttactgaagc aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc
     3841 aaggaacctg ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt
     3901 gaagatgaga ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat
     3961 caaggagata atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca
     4021 caacttgaag aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag
     4081 gaaaaaggat ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc
     4141 gtaaagaaga cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg
     4201 atctgcaaaa agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa
     4261 gcgaattgct ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa
     4321 accatatttg ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac
     4381 cagatatgac tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata
     4441 aaaagcagag caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat
     4501 acggcgatct tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg
     4561 gctttatcga tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa
     4621 gattgatcgt ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc
     4681 atacgatgtt tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca
     4741 gtggggatgt gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc
     4801 taattgcaca tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc
     4861 atctttatat caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat
     4921 ttccgacttt agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata
     4981 tccaattaga aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc
     5041 agaaaggatg agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga
     5101 aaagaagacc gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca
     5161 gaagcaaata cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg
     5221 ccgaatcgtc agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat
     5281 gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc
     5341 atcgctggag ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt
     5401 acagtcatcc atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat
     5461 tggtcactga tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa
     5521 tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata
     5581 taaaaaagct atggatagac tagctgttat ttagagctgt cccatccata gctttatttt
     5641 ttttattaag taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac
     5701 aaatgccaaa caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc
     5761 gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa
     5821 agaggaacag cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact
     5881 tttgatactc tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca
     5941 aacaaccaga ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa
     6001 aaaggagtat aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac
     6061 accttttttg cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga
     6121 atcatcatcg atccatagat agcataagag acaatatgga gggaggaatg caagtgggca
     6181 ccttttacta aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg
     6241 actgcattaa acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg
     6301 aaaacggctc ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa
     6361 aatagaaatt gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag
     6421 agagaaaata atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa
     6481 tgtacgcgat gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt
     6541 atatcccgat gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa
     6601 aaatctgccc aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct
     6661 aggagaagac ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt
     6721 agaagcagca agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga
     6781 taccactgac caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg
     6841 taaaacagta gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata
     6901 catcggaata tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg
     6961 tttgctgtct agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat
     7021 ccctgtaact aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa
     7081 tgaactgaaa gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga
     7141 attggcgcaa gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt
     7201 actgcatacg aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta
     7261 ttatgtagat tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct
     7321 ctttttcttt ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt
     7381 gataa
//
LOCUS       DACEND010000124         7366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEND010000124 DACEND010000000
VERSION     DACEND010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.127"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW746_002228"
     CDS             complement(150..1337)
                     /locus_tag="IW746_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1079118.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1727..2251
                     /gene="infC"
                     /locus_tag="IW746_002229"
     CDS             1727..2251
                     /gene="infC"
                     /locus_tag="IW746_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1079119.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2323..2523
                     /gene="rpmI"
                     /locus_tag="IW746_002230"
     CDS             2323..2523
                     /gene="rpmI"
                     /locus_tag="IW746_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1079120.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            2576..2935
                     /gene="rplT"
                     /locus_tag="IW746_002231"
     CDS             2576..2935
                     /gene="rplT"
                     /locus_tag="IW746_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1079121.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            3187..4494
                     /locus_tag="IW746_002232"
     CDS             3187..4494
                     /locus_tag="IW746_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1079122.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            4571..6700
                     /locus_tag="IW746_002233"
     CDS             4571..6700
                     /locus_tag="IW746_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1079123.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            6675..7364
                     /locus_tag="IW746_002234"
     CDS             6675..7364
                     /locus_tag="IW746_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079124.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         2338 a         1365 c         1568 g         2095 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatccggga atcactgcta gaaagtctgc taatctaatc attttcagaa aaggaagaca
     1501 aactgtatga aaaacattga caaaaagatt agtttttgct atactatgat ggttgagaaa
     1561 taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct agatagtttt
     1621 cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt tttttattgc
     1681 cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga ccatagcaaa
     1741 ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg gttcagatgg
     1801 tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac aagcaaactt
     1861 ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca tggactacgg
     1921 taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac aaaaagtgat
     1981 caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca atacaaaact
     2041 tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta tccgtttcaa
     2101 aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct tagctgaaga
     2161 aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca gcatgttctt
     2221 gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct gttggacagc
     2281 cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa acaaaaaaca
     2341 caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa acgcttccgc
     2401 gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca attgcgtaaa
     2461 gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc aaaaatgaaa
     2521 taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat tatacatggc
     2581 acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa aattagcaaa
     2641 aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag taatgaattc
     2701 atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta aattgtggat
     2761 tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat taatgcacgg
     2821 tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg ctgttaacga
     2881 tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta aataagctaa
     2941 acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt tttttattta
     3001 caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaatagc ataattaagg
     3061 ttttttaatt gaaaactgaa catttatggt caccctttaa tgaaagcgct aactacataa
     3121 gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa tggacggagg
     3181 aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt tttcatggtc
     3241 tggtactgtg taaaaggcta caacttgatg gtcggctttg ccatcatggc tactgtatgg
     3301 atgggattag cgctagtcgg aaacacgttt tcaccaaacc cggcgatgga agggcaagga
     3361 gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc caaatcgatc
     3421 ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag cggaatcgca
     3481 gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat cacgatgagc
     3541 ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg accagtgatt
     3601 tctattgcag ttattgtgtt gccaatcttg atgtcattag gtatttcagt accggttgca
     3661 ctgttctcat ttatgggctc gattatggct ggaatctttg ctaatatcgt caatttcaaa
     3721 caatatcaaa cgatttatgc tggtttcaat ccagcggctg aaagctatac ttataatgac
     3781 tatttccaaa ttggtatgat tgggatgatt gtaagcttag ttgtggtact aacggtcgct
     3841 aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag caaacgtacc agctgaaggt
     3901 ggcgacgcac caatgatctc atggttagcc gtattattac cagttttagg ggtcgttctt
     3961 ttagatcttc caatcatctt aggttttatt ctagcaggta tctgggcatt gctgtttact
     4021 ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt ttgcgaaatt atttacagat
     4081 ggcgcagtag atgttgcacc aatggttggt ttcttgatga ccttagcgat gttcaataat
     4141 tcagctgcgt atgcttctcc ttatttttca gctatttttg gagattggat accgcaatca
     4201 ccattagtct tggcaatcgt atttgctata ttgacaccac taggattttt ccgcggtcca
     4261 atgaatctag tcggttcagg ctcagctatt ttagctgtcg tattagctgt taatccaacg
     4321 atgtcgccag cctttttgtt cccgttattt gcaatcacaa cgattgctcc tcagcattta
     4381 gatattacac aatcttgggt tgcttgggga ttaggttata caaaagtaac ttctcgagaa
     4441 tatatgaaga aatccatccc aactggttgg atcatcggag cgatctttgc ctgatcacgt
     4501 tcttattgta cgggaacgcc tagtaaacaa aacgtcgata aaaacgatta gatgaatgga
     4561 ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat gttggcggta cacatacgaa
     4621 agcagtcgca atcgataatg cgacgcacga aatcatcgga aaatcttcag tgaaaacaac
     4681 ccacgatgat gtccgtggag tagccgcagg tgtcgttcag tcttttcaaa attgtttgcg
     4741 ggaaaataat atcagtccag aagatgttgt gtttgttgca cattctacca cacaagcgac
     4801 taatgcactg attgaaggag acgtagcaaa agtcggcgta atcgggatgg caaaaggtgg
     4861 attagaaggt tttttagcaa aacaacaaac tagattgaac gatatcgatt tagggaataa
     4921 gaaaaaaata gagattgtca atgcattctt acctgtcaaa cacttaaatg tagaccgtgt
     4981 ttcagaaacg atttcttctt tagaaagaga aagagcggag gtcttggttt cttctatggc
     5041 atttggagta gataatgggg aaccagaacg agttgtttat gaagcagcat cggtaaaatc
     5101 gatccctaca acgatggctt ctgacatcac aaaattatat ggattgacaa gaagaactcg
     5161 gacagcagca atcaatgcat ctattttacc aaaaatgcta gatacggcaa cctctacaga
     5221 agattccgta cgagaagcag gggtcaatgt ttctttgatg attatgcgcg gagacggcgg
     5281 agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta accatgcttt ccggtccagc
     5341 agcatcagtc atgggctctt tgatgtactt gagagcgtct aatggtgttt attttgaagt
     5401 aggcggaaca acaaccaata tcggggtcat taaaaatgga cgtccagcaa tcgattattc
     5461 aattgtaggt ggccacccaa catatatctc ttcattagat gttcgggttc taggtgtggc
     5521 aggcggttcg atggtacggg ctaatcagtc aggaatcatc gatgtcggtc cacgttctgc
     5581 acatattgcc ggtctagatt atgcagtatt tacggagaca gaaaaaatca aaggtcctaa
     5641 ggttgaattt ttctctccta aagaaggaga ccctgctgat tacgtcaagg tagtgatgga
     5701 agacggagaa gaagtgacta ttacaaatac atgtgcagcc aatgtccttg gattagtgca
     5761 agaagaacat ttttcttatg gaaatgtccc atctgcacga aaagccatcc aagcattagc
     5821 tgattattgt catacgacgg ttgaagacat cgcagaacag atcatggaaa aatcctatgc
     5881 taagattgaa ccggtgattt tagaattggc agacaagtat cacttggaga aagatcagat
     5941 ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt acttacttta gtaataaaat
     6001 gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt tcttctattg gtgtagcact
     6061 tgcaatggtc cgagatgttg tagaacgtat catcccttcg ccaagtaaag aagatatacg
     6121 atcgttgaaa aatgaagcga tgaataaagc gattgaatca ggcgctacac cagaatcgat
     6181 cgaagtccac gtagaaatcg atccacagac ttcaaaagta acggctatcg ctactggttc
     6241 tacggaagtg aaagcaaccg atctaaccaa ggagatcaca acagaagaag cactagagct
     6301 tgctgcagag gatatgcgat taaataaaaa tgaagtttgc ttattggaaa atacaccttt
     6361 cttttatgta tgcggcgaac agaatcgttc aaaaaatgct ggaagtcttc ggatcatcga
     6421 tcaaaaagga tttatcaagg tccaacgtgg acatgcttca tgtatgaaaa caactgcagc
     6481 caattatatg acagctgtcg aacaactttg ggaagatatg gcagtttatc aaactgaatt
     6541 gattgcccga ccagaatttt atctctgctt aggagcaaga gtctctgatt ttacagcgac
     6601 ggatctggaa caactacagc ttttgatgga tctggaagta tctactatgg aaccggagga
     6661 agaagtgatc gtcgttgccg gaaatatcaa acaaacataa gttagaagag ttactaaacg
     6721 aattacaaca gatccccgaa gaaatttggg gattctacca gtttcggcga gatttatttt
     6781 ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca acaatcaatc gattgtggta
     6841 tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc cgatgtagga gaaatttgtg
     6901 aacaaatggc tgttccaatc gtttcctgtg aatcagagca aataaacgaa cgaatcactt
     6961 ttgcaactta tgcagaagat gaagggattc gattgatgac ggagccgtta gaaaaattaa
     7021 aatgttcagg actagcttcg atctctaaag aaacagcgca agctttgatt attggacatg
     7081 aactatttca ccacatcgaa gcttctgtta agggaattta tacgcaaagc gaaaaaattg
     7141 tgctgtggcg gttgcctttt tatacacatc aatcaaacat tcgtgcattg agcgagatag
     7201 cagcgatgag tttttcaaaa gagatgaatc aaagcgattt ttcgccttat gtattagagg
     7261 cagtcttact ttggccatat aatgaaattc gtagccaagg gatcttagaa gaaataaaaa
     7321 aaatagaaaa aagatgtacg gagtatgatt tagattacaa ataaag
//
LOCUS       DACEND010000125         7121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEND010000125 DACEND010000000
VERSION     DACEND010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.128"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..1257
                     /locus_tag="IW746_002235"
                     /pseudo
     CDS             1..1257
                     /locus_tag="IW746_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="IW746_002236"
     CDS             complement(1364..2395)
                     /locus_tag="IW746_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079125.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="IW746_002237"
     CDS             complement(2402..3232)
                     /locus_tag="IW746_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1079126.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4035)
                     /locus_tag="IW746_002238"
     CDS             complement(3229..4035)
                     /locus_tag="IW746_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1079127.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="IW746_002239"
     CDS             complement(4041..4865)
                     /locus_tag="IW746_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1079128.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="IW746_002240"
     CDS             complement(4855..5955)
                     /locus_tag="IW746_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1079129.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="IW746_002241"
     CDS             6133..6918
                     /locus_tag="IW746_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1079130.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..>7121
                     /locus_tag="IW746_002242"
     CDS             6951..>7121
                     /locus_tag="IW746_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1079131.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQ"
BASE COUNT         2109 a         1675 c         1365 g         1972 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca g
//
LOCUS       DACEND010000126         7110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEND010000126 DACEND010000000
VERSION     DACEND010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.129"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..284
                     /locus_tag="IW746_002243"
     CDS             9..284
                     /locus_tag="IW746_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ1079132.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            393..2219
                     /locus_tag="IW746_002244"
     CDS             393..2219
                     /locus_tag="IW746_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079133.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            2232..2948
                     /locus_tag="IW746_002245"
     CDS             2232..2948
                     /locus_tag="IW746_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079134.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            2961..5819
                     /locus_tag="IW746_002246"
     CDS             2961..5819
                     /locus_tag="IW746_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1079135.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            5870..>7110
                     /locus_tag="IW746_002247"
     CDS             5870..>7110
                     /locus_tag="IW746_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079136.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLA"
BASE COUNT         2669 a          918 c         1405 g         2118 t
ORIGIN      
        1 gaaataaaat gaaaaattta gttatcagca agtttgcaat gttaccaaga atggattttt
       61 tcgataatgt aagtaagttt atgtcaaata tgacttcaaa actacaagga atttcactat
      121 ctgtaattat cttttgtatt gtggttactg gtttgatgtt tcttttcgga gaagggccaa
      181 gtcgtactgc taaaaaatgg ttaacttata tcgtgattgg ggcaataatt atcttcggag
      241 cttcaactat cggacaaacg atcaagtccg ttggtggttt ctaaggggaa gcttcttaga
      301 actaaaaaat tcgcaagtat tgcatgaaag agcattgagt gattaatgct cttttttatt
      361 ttgaaaaact ataaactaag gagatttaaa ctatgaacca acgattcgaa tataattttg
      421 aagatgtaga acctaagtta ctgaaaaaag gaaaaggatt aaaaaagaaa aataaatata
      481 ctctcaaaca agcagaagca ttagtaggat ggattcaaca caattggtca gacaagacaa
      541 caattacctt gatctgttca gacacagaag aaggaacaaa gtatgctttt gatggaattg
      601 aaattactcc attttcgatt ggcggacaag aaaaattatt gtttcctgat attgaaggac
      661 aaattttaca agcgccggac agcatgattt ccgatcctaa taaactgatt ttggatatta
      721 acgatcaata caaacacgaa ccagacgtag cagaaccaac aaaagaatca aagtcaaaat
      781 taaatttgaa gctcccttca gtagtattac catttgctaa gaagaaaaat aaacttatga
      841 acgaattgat tgaaccagaa gaaacggata tttatgaaga agaattagaa gaacaagaag
      901 agatcgaaga attagttgtg tcttctgaag aaacagaaga agtagaagaa attgaaactg
      961 tttctgaaaa tgaagaagaa acagaagaaa tctttgactt tgaagatgaa gaagaagtgg
     1021 aaaatgtaca gaaaggcgat ctatctacaa gtagtcaaaa tgggttggaa gctgtaaatg
     1081 atccacaagt tccaccatat atatcttcac aaactcccaa ttactctcca cagtatcaag
     1141 cgcctaatgt cttacaacca ttaagaaaac acgaaacagt gcagttagtt acgcttaaag
     1201 agtactgtga tttgggagaa gaaattgaaa cttcaatttc tcaaatggtc gaaaagttga
     1261 aaccagaatc tttattcaaa ttcataggga ttccttctac aggtcttaca aatactcgta
     1321 ttgatgaata tagaagtaac catgctatgt tgcgattagc tgaagtaaaa tttgaaaatt
     1381 taagagagca ttattcacgg caaattattt cttttaaagc agaagcccta gatcgtttaa
     1441 gaactgcggt agatattgct tggaagaagc cttacgattt agatgtgaag aaaaacaagg
     1501 ccgaagagtt atcaaaaatg gaagcagaag cagaagctaa attaaatgag ttcgtgaaac
     1561 gtcaggaaaa caatgtaaat gataaaatca aaaagtttga gatcgaacaa gaaatagatt
     1621 taaaaaactt tattgcaaga caagaggcag ataaagcggt atttattgct acagagaaag
     1681 atcggactca aaatttaatt gattctaaag cggaatcaat caatgcagat ttacttgaaa
     1741 caaaagataa atttattgat gaagaaatgt acaaaatgaa aattgaaatg aacaaaaatt
     1801 tatttgatgg caagcaaaaa attaaatcag aattggctac tgaactcgct aaggcaaatg
     1861 ataatgtttg ggaaaatgcg ctgaaattta ttcaagaaat tcaagatgaa attgaagcta
     1921 aaacaccaat ttgggctgct gaaatcaagg agtttaatag tttagaagaa caagaacatg
     1981 aacgtatgaa gcaaactaaa gaacttcaat taaaagagga aagtattgaa atcaagcgtt
     2041 tggaagctga aatgaatttg aagcgtatgg aagaattaga tcatgaaaat aaaatgctta
     2101 aaattaaact tgaaactgcc ctttcaaaaa atgagttatt agctatggaa caagatacaa
     2161 agcaagtggt gaatggatca aacagagttg gtgcacttaa tttatttagt aaacgttaaa
     2221 ggagaacgga tatgagtgtt tttaataata aagttatgtt agggaactgt tatgtgaacc
     2281 acattggttt ttacgatgga ggaacataca gagttttatt cttacgttta tctgttttta
     2341 gttactcaaa agatggaaag gggcattatg aaaatattaa tgcacaaatt tcttgtcctt
     2401 tagatagaaa tggggaaacc acgactacaa gtagtcaccc taaagccttg atgaataaat
     2461 ttatctttga aaagttggat cgcaatttgg ttgaggggat gaagataagt attcatggaa
     2521 atattcgtgg aacagaacaa gtgcttctta acagtggttg gaaaaacttt ggtcaattat
     2581 cgcctttaga aaaagaagaa tatcaaaaaa ttcctaaaaa tgaattagta acaagaaatc
     2641 aaacatatat ctatatagaa gaggtaagta tgccagaaaa agaagaattt agaaagtctc
     2701 aatctaaggt aacaaaagaa gatgtggaag ttgcagaacc gaatcaagca gatgaaattt
     2761 tatccgatga tactgatcca gtgaatgatg aaggagaggt tattgaagtt tttgaagctg
     2821 aaattatcga agaggaagaa gaaattaaaa gttttgataa aagaatgtat cgagagaaac
     2881 caaaaggtta tgtgaatttt gaagatatgt attatgacga atattctaat gaaccaccga
     2941 tcaattagtg ggggtcaaat atgaagctag tacaaaacaa tgaagtgaaa gaaataaaaa
     3001 acggctttgt taaaaactcg aacaaaattt tgggaaagct gaaaaaagta ggtagtttca
     3061 aaaaaaataa atatcatact ggaaaaattg agggagaaga tttaccaaaa agtttacaag
     3121 ataaattgaa tgttccgctt gtatttatag gtgttttttt aggttcaata gtttttctag
     3181 caataacgta cttattgaac tttttaaaag ctttgttagt tgcaatcgga cagtcagaaa
     3241 gtgggctact tggtgtagag ttcgatccta agtttagttt ggcatatcta aaaccacttg
     3301 gggatacgaa acaatacttt atgtgtgctg tggttgcggt aataggagtc gcttatttaa
     3361 tatatgggaa attaaatcat gaaagtgaga aaaatcttgt ttacggtcaa aaaggagatt
     3421 caagactttc aactatcaaa gaaattaaga gagattacca tgagatacca gaaaaaacag
     3481 aagtgtatga tggtattggt ggtgtgccaa ttagtcacta tcaagataaa tattatattg
     3541 atagagacac agtaaatacc gttgttttgg gagcttcacg atccggtaaa ggggaaacat
     3601 ttgttgttcc tttattagat aatcttagcc gagcaaaaat acaaagtaat atggtcgtga
     3661 atgatccgaa aggagaattg tttagcgctt caaaggaaac acttgaaaaa cgaggttatc
     3721 gtgtattagt gctaaatatt gatgatccac ttgaatcaat gagttttaat cctttacaac
     3781 tagtaattga tagttgggct aacaaggact atcacgaagc tagtaagcga gcgaatactt
     3841 taacttcaat gctctttgct tcaggaatgg gtacagataa tgagtttttc tataaatcag
     3901 ctaaatcagc ggtcaatgcg attattttaa cgattgtaga acactgtttt aacaatgatt
     3961 gcattgaaaa aattacaatg tacaacgtgg ctcaaatgct gaacgaatta ggaagcttat
     4021 tttacactga tccaaagact ggaaaagaaa aaaatgcttt agatgaatat ttcaatactt
     4081 taccacaagg gaatcaggcc aagattcagt atggttctac ttcatttgcc ggtgataaag
     4141 ccaaaggttc gattttatct actgcttcac aaggtattga aatgtttaca agtgatttgt
     4201 tcgggaagtt aacaagtaaa atgtctattg atttgaaaga gataggcttt cctaagtcca
     4261 tacaatttaa agtcaatacg aatttagttg gaaagaggat tagtatttca tttttgagaa
     4321 aagaaaatga tgtaattaga ttaattaaga agtatcgagt gaaagtaaaa gctttagggc
     4381 tatgtgtttt aaattttaat gaatatcttc aggatggaga tatgttggat atacgatatg
     4441 aagaagagaa atcgagagca tggtatcgta ttgaatttcc caaaaaacaa gcaaatagtc
     4501 atgatacttc tttagtcact aaaaaattca agtcaggtaa aaattattct gacttagaaa
     4561 tcagcacctt acgattaaaa tacacggatc agccaactgc tgtgttcatg gttattccag
     4621 attatgatcc gtcaaatcac gtacttgcaa gtatttttat cagtcaactt tatacagaat
     4681 tagcaagtaa ttgtaagcaa acaccggata aaaaatgttt taggcgagtt catttcttgt
     4741 tagatgaatt tgggaatatg ccggctatag acaatatgga tgggattatg acggtatgtc
     4801 taggaagaaa tatgcttttt gacttagtga ttcaatccta tagtcagcta gaaacaagat
     4861 atgataaagc ttttaaaaca attaaagaaa attgtcagaa tcatatttta atcatgtcga
     4921 acgatgagga aacgatagaa gagatttcaa aaaaatgtgg tcataagaca acaataaata
     4981 gaagtgctag ttctaaacat ttagatactg atagctcggt cactagttcg gcagagcaag
     5041 agcgagtaat tacagttgag cgtgctagtc aattgattga aggggaacaa attattttaa
     5101 gaaaccttca tagacaagat acaaaaagaa ataagattag accatttcct atttttaata
     5161 ctaaagaaac aaatatgccg tatagatacc aatttttagc agaagacttt gatactcaaa
     5221 gagatattaa tgagattgat attgcatgtg aacatatcaa tctatcttta caagataacc
     5281 aaattccata tgcaaggttt atcaaagatt taaaaacacg tttggagtat tcaattatta
     5341 ataatattcc tattagtgaa gaagactatc aggattatct aaatttatca ggatcacaac
     5401 aaacaattaa tgaagaagag ctgttaaaac tggtaaatac ggaagtaaaa agtcaagata
     5461 tggaaggtca agttttatca gaacaagaaa tgaaaattaa tatttatcta aaggaaagtc
     5521 aaaaggaagc aatagaatta gtaaaagtaa ttaatggaag gttagctgat gaaaattcag
     5581 cagatatggt tataaaaacc attaattcac aaatccttga tacgcctttc attcaatctt
     5641 taatcaatga ttctatagct attcataatg ttcaagagag taaggatcaa attatgatcc
     5701 tagaacaaca attagctaat gcttatgagt tagcagtgtt taaaacgaaa aattcaatac
     5761 tgaaaaataa aattatcaag ttgaaaaatg ctatgactaa gctagcaaaa gcaatttaga
     5821 gagaagggaa gaaatattga ctgaatctat taattggaga aaactaaatg tgcaatggtt
     5881 aaagtataaa tggccaatca tagtttccct aataatcatt ctatttatgg cctatccgca
     5941 acgagttttt gcgttgccgt ggattacaga caataaagag gtattaaaat ttttaacaaa
     6001 atatcaagat tatcttcagt ttactaaagg tggttttgga agcattgtcg ttcaaagttt
     6061 atcgtggggg ctgattagag gtgcatattt tgtttgtaaa tcagcagaaa accttgttcc
     6121 acaatcgcta agtttgttta gctttgttga aagcagtagt ttaggtgctg tttatcaatc
     6181 tgtattaaac acggtggttg taggattaat gatcctttca ttgatgtttg ttggttacaa
     6241 gatgattact ggaaaaggaa cagtggattt aaaatcggtt ggaatgaacg tagtgatgtc
     6301 tatagcatta atacttttaa tgccaaccat gatttctagt ggtattcaat ttgctaaaac
     6361 attttataag gactctacta cgattacaaa agctgatggg ggcgttgctt ggtcattgat
     6421 tgaggacggt gtaactgatt tagtatatat caataaaaac gatgcttatg atcggttggg
     6481 taaagatggc gtacaaaaaa atgcgttaaa agaaaagaat ttcttttcct acgatttcag
     6541 tgaagtaatc acagttgata cagctaaaga tttagaagat gataataaaa aagctgaata
     6601 tcttcaatct aggttgacac cagatggcga aggaaatatg caagccgtta agattgatga
     6661 tggttggatg tcgatgttct ctgatagttt caaaggaggg tactacaggt ttactaaaaa
     6721 tacttttgca ttgactttag gattactttc gttagctgta gcctatgttt ttagtatttt
     6781 tatcatagtc accgctattt tagaactggc atttaagcgt gtgttagggg ttcttgtttt
     6841 tgcaacagat attgagacag gacagcgttc aaaagttgtt ctatcagata ttctacagtg
     6901 ctatttgact gtaggctttc aaggatttgg attatccatg tttgcaatgt ttgtttccta
     6961 tttaggaaca ggcggagggt cttccgttaa tccgtttatt aaggctgtag cttatgtatg
     7021 tgccgttttt gttttaatca aaggcagtgg aacagtcatg agatactttg gtgttgatat
     7081 tggactcaaa gaaggctatg ggcaacttgc
//
LOCUS       DACEND010000127         7026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEND010000127 DACEND010000000
VERSION     DACEND010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.130"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..280)
                     /locus_tag="IW746_002248"
     CDS             complement(<1..280)
                     /locus_tag="IW746_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1079137.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQ"
     gene            615..1049
                     /locus_tag="IW746_002249"
     CDS             615..1049
                     /locus_tag="IW746_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1079138.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1072..1392
                     /locus_tag="IW746_002250"
     CDS             1072..1392
                     /locus_tag="IW746_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079139.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1533..1724
                     /locus_tag="IW746_002251"
     CDS             1533..1724
                     /locus_tag="IW746_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079140.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1795..2052)
                     /locus_tag="IW746_002252"
     CDS             complement(1795..2052)
                     /locus_tag="IW746_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1079141.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2210..2473
                     /locus_tag="IW746_002253"
     CDS             2210..2473
                     /locus_tag="IW746_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079142.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2497..2685
                     /locus_tag="IW746_002254"
     CDS             2497..2685
                     /locus_tag="IW746_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079143.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2768..2953)
                     /locus_tag="IW746_002255"
     CDS             complement(2768..2953)
                     /locus_tag="IW746_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079144.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3168..3539
                     /locus_tag="IW746_002256"
     CDS             3168..3539
                     /locus_tag="IW746_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1079145.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3689..3761)
                     /locus_tag="IW746_002257"
     tRNA            complement(3689..3761)
                     /locus_tag="IW746_002257"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3726..3728),aa:Gln,seq:ctg)
     gene            complement(3807..4235)
                     /locus_tag="IW746_002258"
     CDS             complement(3807..4235)
                     /locus_tag="IW746_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079146.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4295..5053)
                     /locus_tag="IW746_002259"
     CDS             complement(4295..5053)
                     /locus_tag="IW746_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1079147.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5348..5545
                     /locus_tag="IW746_002260"
     CDS             5348..5545
                     /locus_tag="IW746_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079148.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5651..6133
                     /locus_tag="IW746_002261"
     CDS             5651..6133
                     /locus_tag="IW746_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079149.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6191..6787)
                     /locus_tag="IW746_002262"
     CDS             complement(6191..6787)
                     /locus_tag="IW746_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1079150.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2370 a         1080 c         1384 g         2192 t
ORIGIN      
        1 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
       61 attgttgtcc tgagtagatc aggttaatat cagaaattga atttgattct gcaatggcat
      121 tgatcaagga gttatcacca acgtattttt gagaaatcgt tgatagagta tcacctgatt
      181 gaacggtata agcttcgtcc gcatgagcag tcgttcccat gaagaatgca gcgccggcag
      241 ccaaagttgt tccaaaaagt aaagttttaa gtgatgtcat gaaaaatctc ctttattcgt
      301 taaaaagttt tatttgttgt gttcaacgag gaatacttta acacgtgaaa atatttcata
      361 ggtaaaagaa gcatgacaat ttatgacaaa tccacgtttt ttgtaacaga atgatgttcg
      421 ctcgatgtga aaaatgatga ggaatttcac ataatgcaga aataatggga ttagaacagg
      481 tgttctgatg agaaaagaaa tgtataaata acttgaaaga aattgctttt gttcatcatt
      541 ttatttacat attgcgtata ttttttgtat ttttgcttga gatgttacaa taaaagaaaa
      601 aggaggcggt tccaatgagt aaaagatacc agaacatcat ggtagcagtt gacggatcaa
      661 gacaatcgat caaagcgttc gctgaagccc ttgacttagc aaaggacaat gaagcccact
      721 tatttatcgt gtcaatcatc aacaaagttg agctgaccca tagtgcctat gccttttcaa
      781 aaatctatgc agatgaaaaa caaagaacag aagtagccat gctgaaaaaa atcaatgatg
      841 ccaaagagta cggcattgct gagatccacg caatagttga aactggagat ccaagaaatt
      901 tgattgccaa tgtcatccct ggacaagaaa atattgattt gatcgttatg ggagcgacag
      961 gaaaaggtgc tatacaacaa gcattagttg gatcgaccgc ttcttatgtc gtatcacatg
     1021 ccccttgcag tgtgttggtc gtgaagtaat gagataaggg aggaatagaa aatggttcgt
     1081 cctcaagaag tccgtgcgcc aaaagaaaaa atagaaattt tagcaattat tgaagacggc
     1141 acacaaacaa agaagggata ctcgatcgct ttagtaaagt ggaaaggaaa aaaaggtgtt
     1201 gctctgagat gggatggaga taaccaacaa gataaaggat tccctgtcac agctaacggt
     1261 tatcatccag cgtggtttgt tctgcctgat aaatttatgg aattatacag ctatgattat
     1321 gcaagagccg cagcatcaat caaagcatta gataaattaa cagaagagca gaatgaaatg
     1381 gaggaaaaat aaaaaagacg aaccaatcag cctttagaaa actgattagt tcgaggagta
     1441 aaaatgaaaa aaggttattt gggatgaaac tagaataatg cgtataactt aaataaaact
     1501 taaagcaata aaatggcaag agggtgaggc aaatggaagc aggaaaagta ttacaggaag
     1561 taaaagcact atgtgcagat aaaaaatatg aagaagcgaa aagcctgatc gaaaagaaca
     1621 aagaaaattt ggaagaaaag tacccgatcg cacaagaatt tatagaatta aaacaaggaa
     1681 cgatccttca ccgattcaaa catttttttg gtataacaga atagaaaaaa cgattaaaga
     1741 tcggcagaaa tttgctgcat ctttaatcgt tttcttttta aacatgcgag ggattcaatt
     1801 ctgattctta tataaaaaat agatccaaat aataagaaaa attactgaaa gcgtcaattc
     1861 gatgatgtgt gttattaata cagacgttgt ttcccaatgc ctaaaagaaa aaatttgata
     1921 agtaaaatat acagcaggta aagaggccag taaaactgaa aacttctttt ttgtccattt
     1981 tatgtaatag aaaagaatgc ttatcataat aaaaaaagga atataaaaaa caaattgacc
     2041 gatcgcgatc atactattgc ctccgataaa taaagtccat gatacaaaat aacactttgt
     2101 gcgaaatggc gcaagcagtt ttgtggattt atccggtaaa atctgtatcg attttgaaat
     2161 tagaggagaa acttgctata atcaaagtga aagaaggagt gggattttca tggatcatgt
     2221 agtgttagaa atcgatttgc atttgatcaa taatattcgg gtgatttact atgttgtaag
     2281 taacagtgtc gaacaacgtg tactgacgaa taaaataaat ggtattttag caaagaaaga
     2341 cgttcaccga ttcaataatg gtgaaggaag ttattatagt atcccagtag aaaaaattat
     2401 ttatacgaca gtcaaagtca gagaagatct agataccaaa aaagcatatg aacctatttt
     2461 tacaacttat tgattgacag cagatggagg aaaaaaatga atccatatga acaacttatt
     2521 caagatttga tcgatggaaa aatcgaaaag attgatgtaa aaagagaaga attaatggct
     2581 tttcgagaag cgtggctgaa gttggagaat cgtaaatatg tggtgggaaa agcctcactg
     2641 aacggcaatg tgacttatca ttacgatccg acacgtttat tttagtatga agtattcaga
     2701 aaagaaccaa aggaggacga tgcagcagca ttgtcctcct ttgattttac aaaagtgaaa
     2761 gttttactta tttgaataga tttttaaatt tgtttttgac actgtcgata tttacatctt
     2821 ttaaaagatt ttgggctttc tctttataag gtcctaattg ttctttatgt tcttcgataa
     2881 atcgttttgc gccatcgaaa ttgccgttct taatcatttc cttgactttt tgaaataatt
     2941 cttcttgatt cattttgaac acatcctttg ctatcaacat gattttttac atggacaaaa
     3001 cgagaaaaac tgatttatcc acacgtcaat aatatcaaat aacgatcatt tttactaaaa
     3061 aaacactcga ttctcgcttc taaagtagga gaaaaatctc caacttggga cggatggaat
     3121 ttctttaact gacccatata ctgaatatat aacaaggagg tagaaagatg aaagtgatta
     3181 aatatttgat tggactggcg gcgattagtg gtattttgct atttggactg aaatattata
     3241 ctgaaggaag ttatggagaa atccctggta tacttgatca gctaaatcca ttagttgaaa
     3301 aaggagaggt ctatgtaaag acacaaaaac cagaggaagt aaatgaatat ggtacagcaa
     3361 ggtatgtgca gaaagctgct gatgccaatg ggaaagaacg aacggttgaa tttaatggtc
     3421 tttctgtgtt aaaagaaaat cattatttga aaattagcaa taaaggagca cacatagaaa
     3481 cgtatgagga agttcctaga gaaaaagttc cggagaaagc attagcaatt attgggtaaa
     3541 tcagtttttg tattaagaat acgcccgatt ttattattca taatttaatt atgtaaacta
     3601 aatacaagta gtaaaaataa tacaataatc cccctgcaaa aagattgttc ttttacaggg
     3661 ggttcatttt ttcagcaagc acatcaaatt gggatggcag ggttcgaacc tacaattgct
     3721 tgattcagag tcaagtgcct taccagtttg gctacatccc atttattcaa caaaataatc
     3781 atagcacttt cctaaaaaaa cgcaagttat ttttcttgat cggatagttg ttgctttact
     3841 cgtaaaaggc ggcgtactag tttcttttct aatccagcag gaagggcaat cagttctgct
     3901 actgacaaga cggtgtccaa taactgatat tctttgacag gttcattcat aatatgatag
     3961 atgtttgcta aacgttcaat atattcaaag cgagtatact ctgttcgagc aattagaaat
     4021 ttcaataaat cggtggcttt ctcgtactct tgatctgcgt aataaacaag agattgacga
     4081 tacaatttga tttccagttc attgagggta gcaggtctgc gacgctcgtt ccactcatta
     4141 atagagactt taccttgaac atttcccttg cagtcacgga caaaaataat cggaatttca
     4201 tttggatcca tttttttatg tgtgaatttt tccattatct acgctccctt caaaggtttg
     4261 gtatcttgag tataagggat ttttgtttga attactagct tttctccaaa gttagcatct
     4321 gtccacccat tgcagtgaaa atcgccgaaa aatcgttgat cggtactgct cgatttttta
     4381 caccataggg atcgttgaca taaatatttt ctttgtcata tccagtgact acacaagcat
     4441 gaacaagagg agtgactctt acttctccat tatttgtggt ccaagtcata aaatcagttt
     4501 catctgggac ttcaaaattg atagtcacag aagtccagac aggatttcct ttagatagtg
     4561 cttttttgat atcatcgagt tgagcatcgg tttttggaaa agtgtgataa tccgtttgga
     4621 cgatttgctg agcaagggag gcaactgggg gaacatagac gaccatagcc cagtctccgc
     4681 cactgatatc accaacaaat ccttcattag gatcgccatg agtcccatct gcattgaaaa
     4741 cagggacgta atccaattgc tcagctaact ggtttttagt tgtttcataa ccataaaaag
     4801 ataaaagcat agaaagcgaa gtgacttcac agccattttc taaagaagga gaatcaaact
     4861 ggctctctaa tggaacatct agtaaaactt ctgctttctc ttttgtcttt ttcgtttttt
     4921 gtgttgaact tttttgtgca gtgttcattg tatgttctgg agaatggata tgtaggtaat
     4981 aacttgtacc gcttaataaa agagcagctg tgccccctag aaacaatagt tttttgaacg
     5041 tttttttctt catttgaaat ctcccttttc agctgtttat ctttcctatt ataaatgtat
     5101 ttttattgga aatggaagta ttatatttta atatgtagga aataattagt ttttaaggaa
     5161 tatgttttaa ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa
     5221 cgaaaacagt acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg
     5281 agttaaatct ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag
     5341 gattttgatg acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg
     5401 ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa
     5461 agttgcccaa tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt
     5521 tgcaaaatat gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc
     5581 aatttctttg agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag
     5641 gcgagttgtc atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt
     5701 tttatttctt tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt
     5761 ctacttggtg atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc
     5821 tacagcagat gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt
     5881 ggacagtaga ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt
     5941 tcctttttct gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa
     6001 aatgaatttt ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga
     6061 agaattagtt ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat
     6121 tgatccagaa taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt
     6181 tcaaataaaa ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc
     6241 cagccggtgt ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga
     6301 tattccattc ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc
     6361 ccaatccttt atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt
     6421 ctgaccaatc tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca
     6481 cgtcagtatc tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt
     6541 tttgtgtatt tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt
     6601 cccaaagttt ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga
     6661 aagcagttgg gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa
     6721 gtgaaacatt acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag
     6781 ttgtcataat aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa
     6841 tgaagtatag caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag
     6901 ttcaaaaaac aattttaata agaaaaatcg ttatgtaagg attttcttag ttatcgcttt
     6961 cattgttgtg ttttaggaat ttttgcttat ttgaaaaaaa cagctgatat ataattatta
     7021 aatctc
//
LOCUS       DACEND010000128         7005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEND010000128 DACEND010000000
VERSION     DACEND010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.131"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            254..1357
                     /locus_tag="IW746_002263"
     CDS             254..1357
                     /locus_tag="IW746_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1079151.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(1550..2617)
                     /gene="rlmN"
                     /locus_tag="IW746_002264"
     CDS             complement(1550..2617)
                     /gene="rlmN"
                     /locus_tag="IW746_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1079152.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2847..5294)
                     /locus_tag="IW746_002265"
     CDS             complement(2847..5294)
                     /locus_tag="IW746_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1079153.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(5483..6919)
                     /locus_tag="IW746_002266"
     CDS             complement(5483..6919)
                     /locus_tag="IW746_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1079154.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1924 a         1539 c         1217 g         2325 t
ORIGIN      
        1 tttttagata attacaaaaa agcaaaggga atcggtagaa atactaagag gaacaaaaat
       61 tcattattgt cgttttcaat gtttttgtac ttgttcgttt ttaggatact tattgatgat
      121 ttcaaggtgt tcatgccacc ttattcatca cttatcctgt cttaggttca cctctactcc
      181 atctacagcc gcagtcagat cgatcactct ttaaaatttt aacccgtttc aacgaagaaa
      241 gaaggaaatg aatatgagtc aaagtgtttt cttaccaaat tataccattg gaacagatgc
      301 gtatgaaaaa gcaaatggaa tcgtcaaaga atatgggaca aaagtagtgc tgattggagg
      361 gaagactgca ctttcaaagg cagaaaacaa aataaaagta gctttgacac aggaaataga
      421 ggttgtcgcc agtctctggt acgggggtaa ttcaacttat ggaaatattg aacagttgat
      481 ccagcatgaa agtgtccaaa aagcagacgt cctgtttgca gtcggtggtg gccgtgcttg
      541 cgatactgta aaagtcattg gtgaaaaact agctaaacca gtcttcacct ttcccactat
      601 tggttcgaac tgttcgcccg tcactgcggt atgtgtcatc tataacgaag aaggcgtaat
      661 ggacggactc ttctttcctt caaaaccacc tgtacacacc ttcattgata cagcaattat
      721 tgccgaagca cctgataaat acttatgggc aggtattggc gatgctttat ccaaagaagt
      781 cgaatctact ttttctgctc gtggcgatga tctaggctac acggatagat taggaatcca
      841 aatcggaaaa atgtgcattg atcgtctctt gacttatggt acgcaagcat tgaaagatag
      901 tgccgacgat cgtccctcat atgcaattga acaagtcgtt tcggatatca ttgttactac
      961 cggattggtt tctgttttag ttaacaataa ctataattct gctatggcac actcattcta
     1021 ttacggtacg actgttctag aaaaaatgga gggatacctt cacggagcat ctgtcagtta
     1081 tggtgtactg accttattga tgatggatca gcagttcgat ttgtttgata aaatctatgt
     1141 gtttatgaaa aagaaccaac ttccagtatc gttaaccgac atgaatctca atgtcacgac
     1201 cgatttagaa gcaatcttag ataaagccat ggcaacaaat gatattaaac atgtgcctta
     1261 tgaaatcacc cgagaaaaat ttaaacaggc cattcttgac gtagaaactt atcatgctac
     1321 acaagaaagc cagcaaggat ataagaaaat cagttaaata attgctaaaa cccccatcaa
     1381 gcaggctgtc atcaaattga ggacatttct ttgcttgatg agggtttttc ttgtcaaaaa
     1441 aagctttata gctttcgcta tagaatcttt tctcttattt aagttgaaca gttccttaat
     1501 taagaaaact gctcctttta tggtaattga aaaaacgagt aggatacgat tatttggcaa
     1561 ctgttttttt catttgcttg cttcgcaatt gtccgcaagc tgcatcaata tctgtaccat
     1621 gttctttacg tatcacacag tttacgccgt ttttcttcaa tacatcataa aatctcaata
     1681 catcagcttt tggactgcga gaatattgat catgttcact gactggatta tatggaatca
     1741 agttgacata tgctaatttt ttcttatctt ttaatagatc tgctaattgt tgcgcatgct
     1801 ctggtctatc atttacttga ttcaacatga tatattcaaa cgtgattcga cggtttgttt
     1861 tctctaaata ctcatccaca gcttccatca atttctcaat tgggaaactt cggttgatcc
     1921 gcatgatcga tgttcgcacc tcattattcg gcgcgtgcaa ggaaatagcc agattgactt
     1981 gcaagccgtt attagcgaat tccttgattt tgtgtgctaa tccactagtg gaaactgtaa
     2041 tgtgccgagc accaattgcc aatcctttgg catcattgat gatatgcaaa aagtccatta
     2101 cattgtcgta attatcaaat ggctcaccga tccccattac aacgacatgt gaaacgcgtt
     2161 cgccaagatt gcgctcatca aaataatgtt ggaccatcat gatctgtgca acaatttctc
     2221 cagcagtcag atcacggttc tttttgagca gcccgcttgc acaaaacgta cagccgatat
     2281 tacatcctac ttgtgtcgtc acacaaactg acaagccata ttcttgtctc atcagtacag
     2341 tctcaatcat gtttttatca gggagttcaa acaaatattt tactgtacca tccgaagctt
     2401 cttggacgac catctgtttt agaggattga tgataaaatg ctccgataat ttttcgatca
     2461 attgtttcga tagattcgtc atttctgtga aattacttac acgtttttga tacaaccatt
     2521 cccaaacttg ggcagctcgg aattttttct cgccgttttc tataaaccaa ttgattaatt
     2581 cttcattcgt caagccataa atggactgtt tttccaaagt attttccctc tttttctcaa
     2641 agtttcgcta catactatac atgattcatt tccttaatgc aatacttcac aaataaatcg
     2701 aatagaactt taatctcaca aaaactaaaa agacaagaca tcataccgta ttagaaaaga
     2761 tggaaaaaaa ccattttttc ttttggtatc atcatgcctt gtctttttat tggttattcg
     2821 tttttcaacc cttcatcaca caagttttac atgtcattat cgtccggcaa cgtttctgat
     2881 gtttcttcct gctcaatgaa tactttccga ggcttgcttc cttctgacgg gccaacaact
     2941 ccatgagctt ccagttcatc tactaaacgt gctgctcggt tgtaaccgat ccgaaatctt
     3001 ctttggagaa gggaaatact tgccgtttgc atttcaacga caagtgcttt tgcttcttca
     3061 aacagctcat cttgtggctg ttctggagca cctgtcgttt caacttcatc tgtcggcatc
     3121 attttctctt cataatgagc ttcttgctga tctgtaacaa attgaacgac ccgttctact
     3181 tcgtggtcag aaatgaatgc tccttgcaca cggatcggtt tattctcacc cattggcaag
     3241 aatagcatat ctcctcgccc taagagcttt tctgccccat tagaatcaat gatcgtccga
     3301 gaatctgtac cgcttgatac cgcaaatgcc attctggaag gtacatttgc tttgatgatt
     3361 cctgtgataa catccacact tggacgttga gtagctagga tcatatgaat ccccgctgcc
     3421 cgagccattt gcgctaaacg aataatcgca tcttcaactt cattgctggc taccatcatc
     3481 aaatcagcta attcatctac gatcactacg atgaatggta aggttggatg tttcgttccg
     3541 ttttccaagt ttttctgttg aacaaattca ttgtaaccag aaatatttct tactccggtt
     3601 gccgcgaatt tttcatatcg ttcttccatt tcttgtacta ctttttgtaa ggcttgcgct
     3661 gcttttctag ggttcgtcac gaccggcgtc aacagatgag gtatgccgtt gtacatattc
     3721 aattcaacca ttttcggatc gatcatcatc agttttactt catggggctt agcacgcatt
     3781 aatatacttg tgataatccc attaattgct actgacttcc cactaccagt cgaaccagcc
     3841 acgagtaagt gaggcatctt cgaaagatca gccgtttgta cccgtccaga aacatcacgt
     3901 cctaaaggta cttctagtaa tttatctggg tggtttggtt gtgcttcgat gatttctcta
     3961 aaggaaacca tactgatctt tccatttggt acttcaatac caatcagtga tttacctgga
     4021 atcggtgctt ccatccggac atctttcgcc gctaaagcaa gcgcgatatc atctgttaag
     4081 ttcacaattt tacttacttt tacccctaca gccggctgga cttcgaattt tgtgacggat
     4141 ggtcctaagc tggctttgac gaccttggca tcgacaccga aactttggaa agtttgttct
     4201 aatacgccga tatttttttc aattttttta tactcatcac tttgatccac agttggtata
     4261 gaatcaagca aatcaactgt cggtaattca tagtcttgat tttcctgttc tgcttcaatc
     4321 tcaaattcta atgaggtacc gtcatcctct ggcaactcat ctgaaaaacc ttctgctgta
     4381 ttggtctgtt ttggtgtatc tggtactggt ttctctagat tcgcaggatg gatattttct
     4441 tgaaaactgt cgattggaac aaaacttagc tgctccggct ctgtattttc tggttcctgc
     4501 cattcatcag cagtattttc cctttgctgg ctcaccgctt ttttcttctc atattctgca
     4561 gcttcttttt ctgcagcttc ttttgcagct aagcgccttg cttctttcgc ttctgcgcgc
     4621 tgtttcattc tctcttcctt tgccgcttgc tttcgagctt gcttggcagg atcaccttcc
     4681 aataattcct gtactcgttc tccaattgat tggataccat ttaacagctg atagccttcc
     4741 agattactga acaggaatgc tcctcctgcc agtaatagca ctgctgctaa ataacttcca
     4801 ggctgtgcaa tcaagaagta agtaaaatga taaaggattg ctccaatcat cccaccacca
     4861 acgttattgt ctacttgact ttgttttata tcactggcta acaaatccca tgtcgtcccc
     4921 atgatatttg gttccccagt atggagtttg ccaaataaat gggcatgtaa caataataag
     4981 ataccgaaat aaaagagtat gccgccaaac cagcgtcggt ttttaccaat tgaaaactct
     5041 gtattcttaa tgacaatcca aaatcctaat acggctaata aaagacagag gatttggtaa
     5101 gtatttcctg caatgatccg aaagccgttt gcaatcaata ttcctaaaaa acctaatttt
     5161 aaaaagccga agacagcaaa taaaatcaag gcaaacccaa caccaatcgt cagcatttgt
     5221 tcctgctgct gtttttgctt tttcgttttt ttcgtctttt tccttttttt agtaggccgt
     5281 ttcttttgtg ccatttatta tccctcattt catcaagttt cattatacaa aaaaaagtaa
     5341 gggaactaaa gttttgtttg aaagaactga cgctttcttt catttttcag cttattttac
     5401 agtttttctt aatttctaaa tgattttatc aagaaaaggc acggccatat ccagccgtac
     5461 cttcttgttt taagtatgtg aactaaaggt tctctccatt ttcttcaatc agtttttggt
     5521 accagtaata actgtctttc ttatatcgtt ccaaagatcc ttcttgtgtt tcgtcttgat
     5581 caacatatac aaatccatat cgcttttgat aaccgttcag ccagctaagc aaatctgtat
     5641 aagaccaggt gcaatagcct aggacttctg ctccatccga aattgcttct ttgatggctt
     5701 tgacgtggct ttgtaagtaa ttgattcgat agtcgtcatg gatttgttta tcttctgtca
     5761 acttgtcata ctctcctaag ccattttccg taatcagtac cggcatgcgg taacggcttg
     5821 tgatacgacg cagtccgatc cttaatcctt ctgggtcaat ctcccagtcc caatttgttc
     5881 gttcgacaaa tggattctca acttttttat acattccagg aactccagtt tcttctgatg
     5941 aacctttttt tcctgtcgta ttcatttttc cagcaccaac tccgtttaat ggattgaatg
     6001 cattcgtcgc cgtttgatag taattgatcc caagaaaatc tggtttagca gattctagca
     6061 attctgcatc gccagcttca atcgttggtg cccatccttt ttcttctaag aacctcatgg
     6121 cagcaatcgg atattttccc cacatataga catccatcca ataattggcc atcaggtctt
     6181 ctgcattttc agcagccaga acattttcag gattactgtc aattggataa tttggcgtat
     6241 acgcaaagct tggtccgatt tttccgggca tttctaactc gtgaaattta ttgatgacag
     6301 atgcatttgc taaattagca atatggttaa cttcatacat tcgtttcggg tctgtcacac
     6361 ctggcggatg tgctgctaac aaataaccta atgaagtgaa gatattttgt tcattcaaac
     6421 taatccagta atggactttc ccgcggaatg cctcaaataa tgtttctgca taatgagtga
     6481 aatcttcaat gatctgacgt gattcccaac cattatattc atcttgcaat gcttgtggaa
     6541 gatcccaatg atataaagtc aacataggtt caatatcgtt ttcaatcaat tcgtcgatca
     6601 atctctcata aaaagcgagc ccagcttgat tgacttctcc tcgtccatta ggaaaaatcc
     6661 gtgtccaagc tacagagaag cggtaagctt tcagaccttg ttctttcatt aaagcaatat
     6721 cttctttata acgatgatag tggtctactg ccaaatcacc gttcgttcct ttaaatgtct
     6781 ttccaggtat ccgtacaaat cgatcccaga ctgattctcc tttcccatct tcttgccaag
     6841 ctccttctac ttgatacgct gcagaagcag agccccacag aaaatctttt ggaaatgagg
     6901 taagttcttt gtgttccatt catctcttcc tttcctataa ccttcaatcg ttcctctttt
     6961 agaataacac gaaacccctt ccacaacaaa ataaaaatat gtaaa
//
LOCUS       DACEND010000129         6809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEND010000129 DACEND010000000
VERSION     DACEND010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.132"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..304
                     /locus_tag="IW746_002267"
     CDS             <1..304
                     /locus_tag="IW746_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079155.1"
                     /translation="NIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQL
                     MGDGKSELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            318..1007
                     /locus_tag="IW746_002268"
     CDS             318..1007
                     /locus_tag="IW746_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079156.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1132..3483
                     /locus_tag="IW746_002269"
     CDS             1132..3483
                     /locus_tag="IW746_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1079157.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3563..3958
                     /locus_tag="IW746_002270"
     CDS             3563..3958
                     /locus_tag="IW746_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079158.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4283..4900
                     /locus_tag="IW746_002271"
     CDS             4283..4900
                     /locus_tag="IW746_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079159.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4941..5240
                     /locus_tag="IW746_002272"
     CDS             4941..5240
                     /locus_tag="IW746_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079160.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5274..5441
                     /locus_tag="IW746_002273"
     CDS             5274..5441
                     /locus_tag="IW746_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1079161.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5486..6049)
                     /locus_tag="IW746_002274"
     CDS             complement(5486..6049)
                     /locus_tag="IW746_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079162.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6077..6757)
                     /locus_tag="IW746_002275"
                     /pseudo
     CDS             complement(6077..6757)
                     /locus_tag="IW746_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2273 a         1000 c         1349 g         2187 t
ORIGIN      
        1 aaatatctat tctatgtcag atgaagaatt gtataaagaa tttggtgatt tatccgggga
       61 acagttagaa gaaagtattc caggaattag tgactcagat aaaattgttg tcttgtctgc
      121 tggtgggcaa ttaatgggag atggcaaatc cgaattagtt agttataaac aaaatcacag
      181 acagtttatt gatgttaata ctgattccaa cgctatttcg attaaggatc taaaagagct
      241 tttaaatagt aaaagaaatg aattaaagca gataaaagaa actaattcag tggaattaga
      301 gtaaaggaag attcattatg aaaaagattt ttggtgtttc tgttgggcta cttatgatgt
      361 taacatttgg aaatgcagta tatgctgctg agaaagaagt tatttcagat ccagagcagt
      421 acgcctcacg atactttgct gattttgaag aagaagataa agtacttgca ttgccagaag
      481 gtggtttttt aaaaggggaa gctaaaattg tatctatgga taatccagat ataattcaag
      541 gttcatataa ttcagaaact gatcctgctt cagtttcagt gacagaagca aaaaaaattg
      601 ttgctgaaaa attcaaaaat gatattgaaa ctactccaat tatgcctcga ggagcatttc
      661 cacccgatgt tggtactagc ggacaccttc ttgtactagc aggaggagga agctataaat
      721 cacagccatt tagtggtgcg ggatggcgtt ttggaggtat gggtgtaaaa ccagctgatg
      781 gtacaggcaa ctttttacgt tggaaatcct acattgacga tggaagagta ggaaatgtaa
      841 atcaagccaa cgcaactaaa tctggttcta ttcaaggaat ggcattgtat gctcgtgaag
      901 gattcaaata ctttgcacca aacgggcctg gttggacaat gaatataggt atttattaca
      961 cttataatcc tgttgcaggt acgtattatg ttgttcaaaa tatttaataa aacagcacaa
     1021 agagatttct ttgtgctgtt ttattatttt tagttgttga caaacactaa aatataaaat
     1081 ataatcaaca cataaacgaa cgtatgttcg gcaagaaagc aggggatttt tatggatgag
     1141 atggttttaa gtactcagaa atggctaaac aagaaataca gtaatgttac agggtttgat
     1201 aaggttccag aaaatgggcg aacagggtgg cctacaatct atggacttat tgaaggacta
     1261 caagtagaac ttggtattac taatctagta gctaattttg gacctaccac tgaaaaaatg
     1321 tacgataatc aggttactcc taaatggggc aaaaatctac ctaaaaatat tgtatttctt
     1381 attcaaggtg ccttttggtg taaggggatt aatccaggtg gcttcgatgg ggtatatact
     1441 ccatatttag atactgctgt aaaagagttg cagactgatg cagggtttaa tggaagcgct
     1501 gtaactggag ttttggatgc gaaatgggcc aaagcgttat ttgatatgag tgcttttgtt
     1561 cttgttcccg gcggagatag taaaattaga aaaatgcagc aatggttaaa tgtaaattat
     1621 ttagaataca cgggaataat gccttgtgat ggtatttatc aaagaaattc aaatcaagca
     1681 ttaatttttg cattacaagc tgaactagga tattctccca gtgaagcaac agggtcttat
     1741 ggtaatggga caactagtaa aacttatcct gtgtctgaag gtaactcggg aaactatgta
     1801 agaataattc aatatggatt atatgttaat ggttattttg aagacggtac attcgatggt
     1861 attttcacta aatatatggg tttagaagta ctggcattcc gaaaatttat ggtattgcct
     1921 gaatatactg aaatagcaga tgttgttgta ataaaaggac tattgtctag cgctggagat
     1981 attagaagat ctgcagatgg cgcagatact tctacgcaat taactagatc tcaaatccaa
     2041 acattggttg ataatgatat aaaaatagtt ggtagatatc ttactggtac tgttggaatt
     2101 ggaaaagatg aacgaaataa gtatctaact acagaggaat tgaataatat tttttctaaa
     2161 aatttatctg tgtttcctat ctatcaagat ggaggtgcag cattagctta ttttacttat
     2221 gatcgtggct tatcagatgg taagaaggct atagatgcag caaaaaatct aaatatacca
     2281 ttaacaacag ttatttattt tgctgttgat ctagacatgt taggggaaga aattcttgca
     2341 tatgctggag aatattttaa gggggtatct gctgtaatgt catatagcgg gtatcaaacg
     2401 ggtgtttacg gaacaagaaa tgtttgttct caaataatca ataacgggta tgcttcgttt
     2461 gcatttgttt cagatatgtc gactggttat agtgggaatt taggttttcc tatgcctaga
     2521 caatggtctt ttgatcaatt tgttgaattt accataggaa gtggcaacgg agctgttggt
     2581 atcgatttaa tcgctacatc gggtagagat agcggattta atgagttatc aaatgatact
     2641 aataatgata attacattgc caaatacaat cagaaggtta taaatcagat ggtacgtgcg
     2701 tatcaatatc tatcacaagc tggtcttgat aatccttgga accctcattt gacattttat
     2761 agatatgtaa attattctgg tttgtcatgg gacatcattt cttctcctgt aacagagcat
     2821 gatagaaaaa tttatgatga atataggaat aaattaacta atagcgaggg cctgtataat
     2881 tattttatag atcctaatac tggttcaata ataggtttgc ctcacttaat tgtgacgtta
     2941 caatcacaga tgtttctagc cgacacaatc aatgatagtg tttctgactt tgcaggttgg
     3001 ttaggagatt taatgacttg ctggggtgaa gttaagaaat taggaataca aatggagcaa
     3061 ggtgtatttg cattagtagg tacggctgca ggtggaacat ttagtctaga agatttatac
     3121 caagatgttg acgcggtaaa tgtttatacc ttagcaaaaa gaaattctga ttcaacagct
     3181 ttatcggctt taaaaacgta ctattgtgat aagggtgcta ttgatagatt taatcaattt
     3241 gttaagaaca gatttggttc ttcagataaa atcgcttcag taacaaatag tttattagaa
     3301 aatgacgaaa cagttgatat cttaggtgta gggataattt catttttcat tgataaaata
     3361 gttaataatt cacttgggac agattttact tatcttgatt ttattggaga tgaggggaaa
     3421 gttgcaaatg gattttctga aaaaattatt caatttcaag aaaatagaag taaacttaat
     3481 taagaatcgt ttactattta aacagtgtcc ttttcctttt taaatatata cgataaaata
     3541 agtatatgaa ttgtaaaagg acatgatgat tatggaagaa atagaatgga ataatctgac
     3601 tcaaaataat aaaaataatt ctggatgtgg aattatcttt atgtttgtta tagttttggg
     3661 aatagtagta cttttaataa ctgtcaagat agtagattac tttgcgaata ctcctgaaga
     3721 acaaatacag agaaaacttc ctgagtttga aagagtacca ccttatgatc caagagataa
     3781 ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa atgtggtttg aagttacagt
     3841 tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat tatattgtaa aggcaaaagt
     3901 atcaaaaaga tttattttta accctaaatt agaaatatat agtgttgtaa tagaataata
     3961 gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg atgtgaaaaa taaaatatac
     4021 catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga ccgttaaggc
     4081 ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt aaacttactg
     4141 tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa
     4201 ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt
     4261 gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga
     4321 tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat
     4381 atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg
     4441 gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa
     4501 acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt
     4561 gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct
     4621 tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat
     4681 taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag
     4741 aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa
     4801 gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg
     4861 ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac agctattaga
     4921 cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc
     4981 tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc
     5041 aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt
     5101 ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc
     5161 atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag
     5221 aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc
     5281 gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc
     5341 acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact
     5401 gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag
     5461 aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag
     5521 ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct tgctttaaca
     5581 tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta atagaatact
     5641 cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct tgccgctgac
     5701 gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat aaaaacatat
     5761 cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac tgtgcatcta
     5821 atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt atatcttcat
     5881 aatctcttcc taaacgatct agtgatgtaa cgatgacgca atcaccttca cggatataat
     5941 ttagtaattt ttgaaactca ggacgctcag tatctttacc actggctttt tcttggaata
     6001 atttatcaat gttaagtcct tcggttctgt tgcgaagttt gaaaatcaaa cgcgccctct
     6061 ctgaactcca aatatcttag gctgttattc ccattaatac cttgatttca gtagacaccg
     6121 aaaagccgaa gagcgttcca tttcttcggt tctttttata tattcctcga atggtctcca
     6181 tgcccttaat cgtggaagag gctgtacgga gactttgata aaatttattt cgtcgtttaa
     6241 taggtcgatg gtcttgttct attaaattgt taagatactt cacagttcgg tgctctgtct
     6301 tagtatataa acccacactc tgtaactttc taaaggcgga gccaagagaa ggtgctttat
     6361 cggtcacaat tgctttcggc tcaccaaact atttatggag tcgttttaag aaagcatagg
     6421 ctgcttgcgt atcccgtttc tttcgtaacc agatatctaa ggttaagccg tccgcttcaa
     6481 ttgcacgata aagataatgc caacgtccct taattttgat ataggtttcg tccattttcc
     6541 atgaatagaa ggattgtcta tttttcttct tccaaagata atagaggact ttgctgtact
     6601 cttgtaccca acgataaatc gtagtatgac aaacatttat tccacgatca tataacaatt
     6661 cctgaacttc acgatagctt agattgtaac gcaggtagta accaacagcg acaataatga
     6721 cgtctttttt gaattgtttg cctttaaaat gattcattac tctgtcctct ctgtcttttt
     6781 tctcaatttt acactaaaat agatttttt
//
LOCUS       DACEND010000130         6760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEND010000130 DACEND010000000
VERSION     DACEND010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.133"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..405
                     /locus_tag="IW746_002276"
     CDS             178..405
                     /locus_tag="IW746_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1079163.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            421..1587
                     /locus_tag="IW746_002277"
     CDS             421..1587
                     /locus_tag="IW746_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1079164.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1822..2865
                     /gene="hrcA"
                     /locus_tag="IW746_002278"
     CDS             1822..2865
                     /gene="hrcA"
                     /locus_tag="IW746_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1079165.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2959..3522
                     /gene="grpE"
                     /locus_tag="IW746_002279"
     CDS             2959..3522
                     /gene="grpE"
                     /locus_tag="IW746_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1079166.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3573..5402
                     /gene="dnaK"
                     /locus_tag="IW746_002280"
     CDS             3573..5402
                     /gene="dnaK"
                     /locus_tag="IW746_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1079167.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5553..6719
                     /gene="dnaJ"
                     /locus_tag="IW746_002281"
     CDS             5553..6719
                     /gene="dnaJ"
                     /locus_tag="IW746_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1079168.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2308 a         1160 c         1527 g         1765 t
ORIGIN      
        1 tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat
       61 catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat
      121 gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg
      181 aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt
      241 accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt
      301 ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa
      361 cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca
      421 atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt
      481 tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta
      541 gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca
      601 gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa
      661 cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt
      721 actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat
      781 ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt
      841 ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat
      901 tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat
      961 cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg
     1021 atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct
     1081 gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga
     1141 cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca acataacctc
     1201 atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga
     1261 gatattcgtt accgcaacaa aggaccgatc caacactatt tacgcccatt acgagcaaat
     1321 gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg
     1381 ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa
     1441 aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt
     1501 gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg
     1561 tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga
     1621 aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta
     1681 catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc
     1741 ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag
     1801 tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc
     1861 atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga
     1921 gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg
     1981 ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac
     2041 gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag
     2101 tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta
     2161 tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg
     2221 actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat
     2281 atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta
     2341 gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac
     2401 cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg
     2461 atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt
     2521 ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg
     2581 ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat
     2641 attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt
     2701 caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact
     2761 agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct
     2821 aaacttgctg attattaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc
     2881 tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa
     2941 tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat
     3001 gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct
     3061 gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataga
     3121 tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag
     3181 caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac
     3241 ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc
     3301 gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc
     3361 cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg
     3421 actgaagata caccagccga tacgattgta gaagtactac aagaaggcta caaattacat
     3481 gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact
     3541 tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag
     3601 gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc
     3661 cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag
     3721 gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc
     3781 atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa
     3841 tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag
     3901 tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa
     3961 aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag
     4021 ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc
     4081 ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat
     4141 tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact
     4201 acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt
     4261 tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca
     4321 cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga
     4381 cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac
     4441 cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt
     4501 tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa
     4561 aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag
     4621 gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat
     4681 taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc
     4741 caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc
     4801 atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat
     4861 tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg
     4921 ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa
     4981 tcacgatcaa atcttcttca ggtctaacag atgaagaaat cgaacgtatg gtgaaagatg
     5041 cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg
     5101 tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg
     5161 aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa
     5221 acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga
     5281 ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc
     5341 aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat
     5401 aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa
     5461 aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt
     5521 gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag
     5581 tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt
     5641 cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag
     5701 tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg
     5761 ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat
     5821 tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg
     5881 gaggaggcgg tcgatcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata
     5941 cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc
     6001 gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta
     6061 cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac
     6121 gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac
     6181 cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg
     6241 taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag
     6301 aaaacggcgg accatttggt gatttgtatg tggtctttcg tgtagaagaa agtgatatct
     6361 ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt
     6421 tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg
     6481 gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg
     6541 gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac
     6601 aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc
     6661 aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt
     6721 ttttctagta aaaatataaa gtagacttgc ccaaattttc
//
LOCUS       DACEND010000131         6693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEND010000131 DACEND010000000
VERSION     DACEND010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.135"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..677
                     /locus_tag="IW746_002282"
     CDS             45..677
                     /locus_tag="IW746_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1079169.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(679..1584)
                     /locus_tag="IW746_002283"
     CDS             complement(679..1584)
                     /locus_tag="IW746_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1079170.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1669..2322)
                     /locus_tag="IW746_002284"
     CDS             complement(1669..2322)
                     /locus_tag="IW746_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1079171.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2644..3588
                     /gene="mvk"
                     /locus_tag="IW746_002285"
     CDS             2644..3588
                     /gene="mvk"
                     /locus_tag="IW746_002285"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1079172.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3581..4558
                     /gene="mvaD"
                     /locus_tag="IW746_002286"
     CDS             3581..4558
                     /gene="mvaD"
                     /locus_tag="IW746_002286"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1079173.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4571..5656
                     /locus_tag="IW746_002287"
     CDS             4571..5656
                     /locus_tag="IW746_002287"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1079174.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5653..>6693
                     /locus_tag="IW746_002288"
     CDS             5653..>6693
                     /locus_tag="IW746_002288"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1079175.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRT"
BASE COUNT         2057 a         1369 c         1341 g         1926 t
ORIGIN      
        1 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
       61 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
      121 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
      181 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
      241 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
      301 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
      361 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
      421 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
      481 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
      541 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
      601 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
      661 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
      721 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
      781 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
      841 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
      901 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
      961 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
     1021 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
     1081 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
     1141 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
     1201 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
     1261 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
     1321 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
     1381 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
     1441 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
     1501 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
     1561 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
     1621 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
     1681 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
     1741 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
     1801 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
     1861 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
     1921 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
     1981 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
     2041 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
     2101 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
     2161 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
     2221 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
     2281 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
     2341 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
     2401 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
     2461 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
     2521 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
     2581 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
     2641 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
     2701 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
     2761 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
     2821 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
     2881 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
     2941 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
     3001 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
     3061 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
     3121 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
     3181 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
     3241 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
     3301 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
     3361 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
     3421 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
     3481 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
     3541 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
     3601 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
     3661 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
     3721 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
     3781 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
     3841 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
     3901 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
     3961 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
     4021 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
     4081 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
     4141 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
     4201 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
     4261 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
     4321 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
     4381 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
     4441 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
     4501 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
     4561 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
     4621 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
     4681 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
     4741 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
     4801 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
     4861 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
     4921 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
     4981 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
     5041 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
     5101 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
     5161 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
     5221 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
     5281 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
     5341 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
     5401 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
     5461 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
     5521 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
     5581 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
     5641 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
     5701 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
     5761 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
     5821 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
     5881 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
     5941 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
     6001 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
     6061 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
     6121 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
     6181 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
     6241 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
     6301 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
     6361 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
     6421 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
     6481 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
     6541 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
     6601 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
     6661 gggatcgact tagtgaagta tggaagacgc aca
//
LOCUS       DACEND010000132         6672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEND010000132 DACEND010000000
VERSION     DACEND010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.136"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..153
                     /locus_tag="IW746_002289"
     CDS             70..153
                     /locus_tag="IW746_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1079176.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="IW746_002290"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="IW746_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1079177.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="IW746_002291"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="IW746_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1608..2273
                     /locus_tag="IW746_002292"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="IW746_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="IW746_002293"
     CDS             2309..2554
                     /locus_tag="IW746_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1079178.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="IW746_002294"
     CDS             2657..3526
                     /locus_tag="IW746_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1079179.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="IW746_002295"
     CDS             3507..4241
                     /locus_tag="IW746_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1079180.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5186
                     /gene="ant(6)"
                     /locus_tag="IW746_002296"
                     /pseudo
     CDS             4274..5186
                     /gene="ant(6)"
                     /locus_tag="IW746_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5183..5725
                     /gene="sat4"
                     /locus_tag="IW746_002297"
     CDS             5183..5725
                     /gene="sat4"
                     /locus_tag="IW746_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1079181.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5818..6612
                     /locus_tag="IW746_002298"
                     /note="aph(3')-IIIa"
     CDS             5818..6612
                     /locus_tag="IW746_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1079182.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2339 a         1004 c         1393 g         1936 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     4381 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     4441 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     4501 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     4561 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     4621 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     4681 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     4741 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     4801 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     4861 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     4921 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     4981 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     5041 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     5101 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     5161 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     5221 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     5281 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     5341 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     5401 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     5461 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     5521 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     5581 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     5641 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     5701 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     5761 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     5821 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     5881 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     5941 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6001 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6061 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6121 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6181 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgattagcc
     6241 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     6301 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6361 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     6421 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     6481 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     6541 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     6601 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     6661 atctaatctt tt
//
LOCUS       DACEND010000133         6536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEND010000133 DACEND010000000
VERSION     DACEND010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.137"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..354)
                     /locus_tag="IW746_002299"
     CDS             complement(58..354)
                     /locus_tag="IW746_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1079183.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IW746_002300"
     CDS             709..1065
                     /locus_tag="IW746_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1079184.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IW746_002301"
     CDS             1075..1974
                     /locus_tag="IW746_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1079185.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IW746_002302"
     CDS             2491..3222
                     /locus_tag="IW746_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1079186.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IW746_002303"
     CDS             3367..5394
                     /locus_tag="IW746_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1079187.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..>6536
                     /locus_tag="IW746_002304"
     CDS             5502..>6536
                     /locus_tag="IW746_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1079188.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFL"
BASE COUNT         2235 a         1083 c         1322 g         1896 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcgctc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttctta
//
LOCUS       DACEND010000134         6200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEND010000134 DACEND010000000
VERSION     DACEND010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.138"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(186..731)
                     /locus_tag="IW746_002305"
     CDS             complement(186..731)
                     /locus_tag="IW746_002305"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1079189.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(735..863)
                     /locus_tag="IW746_002306"
     CDS             complement(735..863)
                     /locus_tag="IW746_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1079190.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IW746_002307"
     CDS             complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IW746_002307"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1079191.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2164..2643)
                     /locus_tag="IW746_002308"
     CDS             complement(2164..2643)
                     /locus_tag="IW746_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1079192.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3002..3784
                     /gene="rpsB"
                     /locus_tag="IW746_002309"
     CDS             3002..3784
                     /gene="rpsB"
                     /locus_tag="IW746_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1079193.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3883..4764
                     /locus_tag="IW746_002310"
     CDS             3883..4764
                     /locus_tag="IW746_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1079194.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4900..5622
                     /locus_tag="IW746_002311"
     CDS             4900..5622
                     /locus_tag="IW746_002311"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1079195.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5625..6182
                     /gene="frr"
                     /locus_tag="IW746_002312"
     CDS             5625..6182
                     /gene="frr"
                     /locus_tag="IW746_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1079196.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2065 a         1156 c         1256 g         1723 t
ORIGIN      
        1 gaggagctat ttttgcttgg ctgtttttat tacttgatac aggacagctt tttcacaacc
       61 tcttattttg tataaaagtc attctggatt cttgtcagcg tacaaaatgt attctgttaa
      121 ctcttttaaa ttgtttacac aaatatcagc gccggcttgt tggaatctaa gagcaacaga
      181 ctgttttaaa cgatcttttt ccccatctct aatttttggt actcatcata agaaatcccc
      241 attagcgaac taccttcaat tacacctact gtcaacactc cggcattttt cccttcctgg
      301 atatctgaga ccgtatctcc gactttgatt acttcatcaa ccgacttgtt tttgaagtac
      361 tgcatgtttt tatggatcat ataaggaaac ggtcgaccat acccttcgac gtcttcaaaa
      421 gttgccatga agtctggtga atacctttct ttttttgcgg tttctgctac gattgtcatc
      481 ataggagcat taaatccatt cgtggaacca atattgatcc cagcgtttct caaccgttcg
      541 atcgtttcca atgtgtcggg tttcaaggta gaatgcttga ccaaatcttt aaacagataa
      601 tgttcaaatt gttgatacaa ttcctccacc tctttttcct gaggtagttg tccataaata
      661 gctatccatt gttttttgac agaagggagg gaaagaagct gttggatatg atcccatttt
      721 aattttccca tcggttagcg gatttctttg tcagtcagtt ggattccttt ttctagaaaa
      781 gcatttctaa aagcgtggac aggagccata cagccaaagt ccacagttgt tcctgcccaa
      841 tcaaaaatta cagttttaat cattaacgtt ctccttatag tctttgataa ttgctgtcag
      901 ttccaaaata tcttccggat agatctcgcc aatatttccg atacgaaaac attcatgatc
      961 gctgattttc ccgggataga tcacaaatcc attttgcttt aaatactgat aaaaatgatt
     1021 aaaatcaaat cgccagtcct tagggtaaag aaaagaggtg atgaatggcc cctgatattc
     1081 ttgaatgaaa ggctggaacc ccatctctgt cattttttgt cgtagcacat gattattggc
     1141 gagataacgt gaatatcgag tttcgattcc tccttctgcc tctagttcct tcagcgcttg
     1201 ttgaaaagcc aaaaccgtat gggtagggga agtgaaacgc catttgccat tctcgttttc
     1261 catcacgcgg tactggtcat atagatcaag tgacaaactt cggctaacat tttttcgttt
     1321 ttccagttcc gctttattag caagtacaaa agaaaagcct ggaacacctt gaatacattt
     1381 gtttgagctg gaaatcaaaa aatcaatccc tagttcggct acacaaatcg gtatcccacc
     1441 aaagcttgac atcgcgtcca ctataaacaa aagatctctt tcttgtgtga cagcagcaat
     1501 ctcttccaaa ttattcaaaa taccggaagt agtctcgcta tggaccattg ctagtgcgga
     1561 gatagacgga ttctcttcaa tcagctgtgt aatcacatga gcttggagga tttgatgttc
     1621 cgatacctca taaacggtat agttcagttt cagacattcg cacatgttga tcattcgttg
     1681 tctatatgcg ccgtttgaac aaatcagtac agtatcttct ggcctgatca cagaacttaa
     1741 taccgattct accccgaagg agctgctacc ttgcattaag accgttgtgt atattccttc
     1801 cgatacttga gcaagatcca ataatttttt tcttatccat tgtgtaattt ctttgtattc
     1861 ttgatcccaa gtacaatgat cgatcagcat agcatttttc actgtttcag aagtcgtcaa
     1921 tggtccagaa gttaataatt tatacatatg atttcctttc atttcttttt tgaaatacac
     1981 aaagagcaag aagctgctct tgttaacatc caatataaat gatgattatt gtgattcaat
     2041 caaggtgttg aaaaatttga ataaaaaatg atgtattttt gtaaagagtg taaaggttta
     2101 ttcaataagg aaaatattga aaataaaaaa acacgcattc catattagga atacgtgctc
     2161 tgattaaaaa agtgatactt cagttggatg gtgaagaaat tcttctactc gttttgcgtc
     2221 ctcctcattt tccgagatga ccatgatcgt atcgaatcca gcaattgtac tgacaataga
     2281 gggaggtttg atctcatcta aaacagatgc aaataaatga gcattgtctg gcaaggtatt
     2341 gacgatcgtc aaaaagtgga ctcgttcgac tttcaataca atgtcttcaa tcatttgaac
     2401 cagccgatcc tcttcctcag tcctagtctc aggagttcct ttttcgcgaa acaatacgaa
     2461 gcgggaaacg ccattttcat caggtgcctt cacgattttt agatcacgga tatctcgaga
     2521 aatcgttgcc tgcgtggcgg taacgccata atgcccaagt ttgtttaata gttcttcttg
     2581 cgtacggatc gggaattctt caatgacttg tttaatcagc aaatgtcttt gagctttgcg
     2641 cattttcttt ctcctttacg aaagatttca catgtatcat aacaaaaaaa agaaggattg
     2701 cccaataata ttcgtctttt atacctaaaa ttattttatg gaaaaaggat aataaagaaa
     2761 caaagagaaa tctactgttt ttcttgaatt ctttttatct ttcaggtaaa ctattgaatg
     2821 ttgagcagaa aagtatattt gctcttgcga tacggatctt ttgtctgtat aatagctgag
     2881 ggtgctttat gcatcagcaa atccacattc ttattggatt gtcggtaggt gcttgaaaaa
     2941 gtttgccggc aaggtagaag ataagaagag gaaaaataaa accaaatcgg aggaaacaaa
     3001 catggcagta atttctatga aacaattact agaagccggc gtacactttg gtcaccaaac
     3061 tcgtcgctgg aatccaaaaa tgaagaaata tatcttcaca gaaagaaacg gaatctacat
     3121 cattgacttg caaaaaacag ttaaattagt agatgcagct tatgattaca tgaagagcgt
     3181 tgctgaagaa ggcggcgtag cattattcgt aggtacaaaa aaacaagcac aagaagcaat
     3241 caaagaagaa gcaactcgtg caggacaata cttcgttaac catcgttggt taggcggaac
     3301 attgacaaac tgggatacta tccaaaaacg tatcagccgt ttgaaacaaa tcaatgcaat
     3361 ggaagaagat ggaacttttg aagttctacc taaaaaagaa gttgctggtt tgaacaaaga
     3421 acgtgaacgt cttgaaaaat tcttaggcgg tatcgctgat atgccaagaa tcccagatgt
     3481 aatgtacatc gttgacccac gtaaagaacg tattgctgtt caagaagcac acaaattaaa
     3541 tatcccaatc gtagctatgg ttgatacaaa ctgcgatcca gatgaaatcg acgtagtaat
     3601 cccttcaaac gacgatgcaa ttcgtgccgt taaattgatt acttcaaaaa tggctgatgc
     3661 tttcatcgaa ggaaaccaag gtgaagatca agtagttgaa gaagatttca cagaagaaaa
     3721 cacagcaaca tcaatcgaag aaatcgtaga tgttgttgaa ggcgacaatt cttcagcaga
     3781 ataattttga atgatggagc tgtttcaaag gctgggcgac aagcctcact ctcctttgaa
     3841 gcagcttttt ttaaaaagaa aaacacacag gaggagttaa acatggcaga cgttacagct
     3901 aaaatggtaa aagaactacg cgaaatgact ggcgtaggaa tgatggatgc gaaaaaagca
     3961 ttagttgaag tagaaggcga catggaaaaa gcagttgatc ttttacgtga aaaaggaatg
     4021 gcaaaggctg cgaagaaaaa tgaccgcatt gcagctgaag gattggcttc agttgctgtg
     4081 aaaggtaaca ctgcagcaat cgttgaagta aactcagaaa cagactttgt ttctaaaaac
     4141 gaaatgttcc aagatttagt aaaagaaatt gcagaacttg ttgctgaaaa caaaccagca
     4201 gacatggaag ctgcaatgaa aatcaaaaca gataaaggta caattgaatc tgacttgatc
     4261 gaagcaactc aagttatcgg agaaaaaatc agcttccgtc gctttgaagt tgtagaaaaa
     4321 gacgacaatg ctgcatttgg tggatacttg catatgggcg gacgtattgc cgtattgact
     4381 gttctagatg gaacaactga cgaaacagtt gcaagagacg tagctatgca cgtagcagct
     4441 atcaatcctc gttatgtaaa cgaatcacaa attccagaag ctgaattaga acatgaaaaa
     4501 actgttctta cagaacaagc attgaacgaa ggcaaaccag cgaatatcgt tgaaaaaatg
     4561 gtagaaggac gcttgaagaa attcaaagca gaaatcgctt tggtggacca accattcgtt
     4621 aaagatcctg atatgactgt tgaaaaatac gttgcatcaa aaggcgcaac tgttaaaaca
     4681 ttcgtacgtt tcgaagttgg cgaaggtatc gaaaaacgcg aagataactt tgtagaagaa
     4741 gttatgagcc aagtgaaaaa ataattctgt tgaataacag acgggaacgc acggtttgtg
     4801 cgctcccttt ttttaagcaa aacaagaaaa accagtctta gagtgtcgaa atcatctaag
     4861 tatggtaaaa tggcaataga aaatattgga ggaatgaaca tggtcacacc gaaatatcag
     4921 cgtgtagtct taaaattgag tggagaagct ttagctgggg acgcaggatt tggaatcaaa
     4981 ccaccagtaa tcaaagaaat catccaagaa atcaaagaag ttcacgagct gggcgttgaa
     5041 atggcaatcg ttgtgggcgg cggaaatatc tggcgcggac aaatcggagc acaaatgggc
     5101 atggaacgtg ctcaagctga ttacatgggt atgttagcta cagttatgaa tgccttagct
     5161 ttacaggata cgttggaaaa cgtgggtgta ccgacacgcg tgcaaacttc gattgaaatg
     5221 cgtcaaatcg cagagccata tattcgccgt aaagcagaac gtcatttaga aaaaggacgg
     5281 atcgttatct ttgcaggcgg taccggcaac ccgtacttct cgacagacac aacagcagca
     5341 ttacgcgcgg cagaaatcgg cgcagatgtt atcctgatgg ctaaaaacaa tgtagacggt
     5401 gtttattctg ctgatccgaa agtagatgca aatgcagtaa aatttgaaga actgactcat
     5461 ctggaagtta ttgccaaagg attacaagtc atggattcaa ctgcaagttc actaagcatg
     5521 gataatgata ttccactgct tgtcttcaat ttaaatgaac acggaaatat tcgccgtgca
     5581 atcttaggcg aaaatatcgg aacaactgta agggggaaat aaaaatggca gatgcaatta
     5641 tgacagaagc aaaagaaaaa atgaaaaaag cagcacaaaa tcttcaacgt gaacttgggc
     5701 aaatccgcgc aggccgtgcg aacgctagcc ttttggaccg tattactgtg aactactacg
     5761 gtgcgccaac accattgaac caaatggcat caatccaaat tccagaagca cgtgttttaa
     5821 tgatcacacc ttttgacaag agcattcttc aagatgttga aaaagctatt atggcaagtg
     5881 atatcggaat cagtccaacc aatgatggaa atgtcattcg cttagttatc ccgcaactga
     5941 cagaagaacg cagaaaagaa ttagctaaag atgtgaaaaa agaagcagaa aatgcaaaaa
     6001 tcgcgattcg taacatccgt cgtgatgcga tcgatgaata taaaaaacaa caaaaaaatg
     6061 gtgatattac cgaagatgat ttgcgcggat tagaaaaaga cgtacaaaca ttgacagata
     6121 acagcatcaa agaagtagat aagatcgctg ctgataaaga aaaagaatta ttggaagttt
     6181 aataaaatca taaaaacaga
//
LOCUS       DACEND010000135         6086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEND010000135 DACEND010000000
VERSION     DACEND010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.139"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..588
                     /locus_tag="IW746_002313"
     CDS             70..588
                     /locus_tag="IW746_002313"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1079197.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            716..1399
                     /locus_tag="IW746_002314"
     CDS             716..1399
                     /locus_tag="IW746_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1079198.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1708..1781
                     /locus_tag="IW746_002315"
     tRNA            1708..1781
                     /locus_tag="IW746_002315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1742..1744,aa:Arg,seq:tct)
     gene            1940..2299
                     /locus_tag="IW746_002316"
     CDS             1940..2299
                     /locus_tag="IW746_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1079199.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2422..4053
                     /locus_tag="IW746_002317"
     CDS             2422..4053
                     /locus_tag="IW746_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1079200.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4115..4756)
                     /locus_tag="IW746_002318"
     CDS             complement(4115..4756)
                     /locus_tag="IW746_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1079201.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5038..5346
                     /gene="rplU"
                     /locus_tag="IW746_002319"
     CDS             5038..5346
                     /gene="rplU"
                     /locus_tag="IW746_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1079202.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5373..5717
                     /locus_tag="IW746_002320"
     CDS             5373..5717
                     /locus_tag="IW746_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1079203.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5730..6023
                     /gene="rpmA"
                     /locus_tag="IW746_002321"
     CDS             5730..6023
                     /gene="rpmA"
                     /locus_tag="IW746_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1079204.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1901 a         1014 c         1294 g         1877 t
ORIGIN      
        1 ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata aagaaagaag
       61 ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac gttgattata
      121 tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt gactccttat
      181 attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat atatgtacga
      241 ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga aaattcagga
      301 aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga tttttataca
      361 aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa tattatcggt
      421 agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc atttgcatgg
      481 attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc atggaatacc
      541 tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga atgttaaaaa
      601 cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag aaaacactca
      661 tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga gtagtatgcg
      721 caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta tgcgtaccca
      781 gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg tagcagaagt
      841 accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac agcttcagcc
      901 tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca tggccacgac
      961 aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga tcgagaaagc
     1021 taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg aaggtcaagc
     1081 agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg atagtgcaaa
     1141 atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag gtggcgtttt
     1201 gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag agattccccg
     1261 tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga tggatcatcc
     1321 cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga caaaggaatc
     1381 agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga ttgaatcgag
     1441 ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa aggatacagg
     1501 aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca attgataacc
     1561 ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt tgggccaaaa
     1621 taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat ttaattatct
     1681 ataattattg atgttccgct gattcttgtc atagtagctc agctggatag agcatccgcc
     1741 ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac cgttagaaac
     1801 tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt tcaaaggcaa
     1861 ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa taaaactcat
     1921 cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc tagttgcaat
     1981 tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca cgacaagtag
     2041 ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat tgtttaagaa
     2101 ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt atctttcttc
     2161 cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg cattatatgg
     2221 cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc tgttggcatt
     2281 gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg aaactttgtg
     2341 aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt tagaaaattt
     2401 catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa aaaagaattt
     2461 tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac agtagctgct
     2521 gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt
     2581 ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg tttttatcaa
     2641 atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc aacaaaggga
     2701 atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc agcaggactt
     2761 ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg gtttacttca
     2821 ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt tcactcggaa
     2881 ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc gataaacaca
     2941 gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg
     3001 gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt tatgctattg
     3061 ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag tagtaatcgt
     3121 ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc agctattagc
     3181 attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca
     3241 aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa tctgttgctt
     3301 cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa
     3361 tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact gctctccatg
     3421 cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg tgttttgatt
     3481 gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac taataaatat
     3541 tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt tgatttaagt
     3601 attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct
     3661 atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga aacggaaaag
     3721 ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc ttcagaaaaa
     3781 ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc catgcgaggc
     3841 gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga aaaagcagag
     3901 caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa acaactttct
     3961 cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc gttgacagct
     4021 ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca gacaagactg
     4081 taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta
     4141 gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa atagctgctg
     4201 gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca tccttttgtt
     4261 ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta accgtatatt
     4321 ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat tttggctgga
     4381 ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa cgagtaggtg
     4441 tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga tgagtcacgc
     4501 gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt aagccagttg
     4561 aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa
     4621 tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga ggtccttgcc
     4681 cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct tgtatcagtt
     4741 cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata aaagaaacac
     4801 aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta ttgcttgaat
     4861 ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc tggtaagcta
     4921 gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa taagcacagc
     4981 atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct ttatagcatg
     5041 tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca agcaatctat
     5101 gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt tatcttagta
     5161 ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt tgaaggtact
     5221 gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc taaaaaacat
     5281 tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga cgcgatcaac
     5341 gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt aagcgaaatg
     5401 acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt ccttatggaa
     5461 gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac gggattgctt
     5521 cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt tatctttata
     5581 ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt ctattagaaa
     5641 atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc caaattaaaa
     5701 caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta caattatttg
     5761 cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca aaacgtctag
     5821 gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac cgtcaacgcg
     5881 gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa
     5941 aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt
     6001 atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg acaagacttt
     6061 tgtcacagcc ttttttgttt ataaaa
//
LOCUS       DACEND010000136         6004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEND010000136 DACEND010000000
VERSION     DACEND010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.140"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW746_002322"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW746_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1079205.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGTGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IW746_002323"
     CDS             complement(2203..4389)
                     /locus_tag="IW746_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1079206.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW746_002324"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW746_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1079207.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IW746_002325"
     CDS             complement(4611..5051)
                     /locus_tag="IW746_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1079208.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IW746_002326"
     CDS             complement(5125..5664)
                     /locus_tag="IW746_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1079209.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1551 a         1351 c         1054 g         2048 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgtcc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctca
//
LOCUS       DACEND010000137         5602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEND010000137 DACEND010000000
VERSION     DACEND010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.142"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW746_002327"
     CDS             complement(57..350)
                     /locus_tag="IW746_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1079210.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW746_002328"
     CDS             complement(378..1043)
                     /locus_tag="IW746_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1079211.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW746_002329"
     CDS             complement(1183..1815)
                     /locus_tag="IW746_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1079212.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW746_002330"
     CDS             complement(1822..2889)
                     /locus_tag="IW746_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1079213.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW746_002331"
     CDS             complement(2886..3617)
                     /locus_tag="IW746_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1079214.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW746_002332"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW746_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1079215.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW746_002333"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW746_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1079216.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1592 a         1193 c          854 g         1963 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tc
//
LOCUS       DACEND010000138         5564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEND010000138 DACEND010000000
VERSION     DACEND010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.143"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(71..1024)
                     /gene="menA"
                     /locus_tag="IW746_002334"
     CDS             complement(71..1024)
                     /gene="menA"
                     /locus_tag="IW746_002334"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1079217.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1037..2140)
                     /locus_tag="IW746_002335"
     CDS             complement(1037..2140)
                     /locus_tag="IW746_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1079218.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2230..3165)
                     /locus_tag="IW746_002336"
     CDS             complement(2230..3165)
                     /locus_tag="IW746_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1079219.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3467..5401
                     /locus_tag="IW746_002337"
     CDS             3467..5401
                     /locus_tag="IW746_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1079220.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1613 a         1091 c         1110 g         1750 t
ORIGIN      
        1 aggagctatt tttatttggc tgttcttatt acttgatgca gggcagtttt ttcacaacct
       61 cttcatgctg ttaaatggct tgcataatga ttgttagtaa tagccccaat acataactgg
      121 cattgaataa gaccatattt ttgatggaat agctgaaact gataggctgg ggtaacgttt
      181 gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt caagaaggtg accaagatcg
      241 gccattcata gataccgctc aacaacccca ttaaaatcac gaaatagcaa gcatacatca
      301 atagctggaa taagactgtt cccgcaggac gtccgatata aaaaactagt gtatagcgat
      361 gattttcaat atcacggtca agatcccgca aattattagc caacatgata ttggcaatcg
      421 taaacaccaa gggtaaggaa gctaatacga ttgctgctat agcccaaaga tttcctacca
      481 aatcaaattg tccagatgaa aagttgactg tcagatcata gacatctgaa ccgaatgtat
      541 tgacataaac gaccatggca aaaattccaa ggcccatagt caccccactg aaaatttccc
      601 ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata gaatactcca ataaaacaac
      661 agataaatcc catcaaaagt aaaagccaac ccgtattgat cgttagaata acgccgataa
      721 caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat caatacagga gatagatttc
      781 tgcggccaat gacattttct tcatacttat atacttcaga atgagccttt tcataatcca
      841 tgtaattatt gatagcggta gtcgctaaat caaaaactag cattccaata aaaaataaaa
      901 aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga gaataatacc cctaccgcaa
      961 aaggaaatac actagccacc tttgttcgta tttcaactac ttctaaaaag atttttagtg
     1021 tcatggttcg tactccttat tttagttcag aacggtcacc catcgtgtaa tgggagtcct
     1081 ccgataattt gaaggtgtct ttgattggat cggtcacata tactttatcg tcttttgtca
     1141 cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac gtattccagt ccttttttca
     1201 cacccattga aaagactgct gtagacaacc cgtcaccatc aattgattta tctgttatga
     1261 tcgtgacact tgcgatatca ttgtcgaacg gataacccgt ttttggatca aacaaatgat
     1321 ggtatttttt gccatctact tctaaataac gctcgtaaat tccagaggta actaatgttt
     1381 tgttccgttc tttaatagaa cctaaaacag atccgcgagc catgttcgga tcttggatgc
     1441 cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc taagacatag acattaccgc
     1501 ccaagtcaac gatggcagtg gtcactcctt gttttttcaa gactttcact acttcgtccg
     1561 taatgtaacc tttggcgatt gcacctaaat cgatgatcat tcctttttct tttagataaa
     1621 cagttttttc tttgtcattt agttcgatct tatgataatc cactaatttc aatgcctgat
     1681 cgatttcttc ttgggaaggt tttctggcgt catcaaaacc gattcgccat aattgtgtga
     1741 ttgcaccaat cgccatatcg aatccgcctt gagaatcttg gctatattcg taggcccgct
     1801 tgactaacgt gtagacgtca tcagaaactt ttactggttt gatcccagct tgctcattca
     1861 cttcatcgat ctcagaacct ttttgattga tcgtgatttt atcaccaagt tcttttactc
     1921 gatcgaaggc aggtttcaat gctgattctt tgccttcatc atacacacgg atacgaacgt
     1981 aagtccctaa caagaactgt tcatcagaat atggatcttt caatatcttg gtagctgctg
     2041 aactttcagt ggttgtttta tttgctgagt tgctgcagcc agttagcacc ataaaagaga
     2101 tgcccagtgt gattaaggaa aatagtagtt ttttccgcat ctttttcctc tcttcatttt
     2161 gaaaggtgag acgactgcct aatctggcta tcgtcccacc ctccaacttt tattagtatg
     2221 attatgtgat tatttttctg ttacgatatt atctacttcg attgtttgag tgtcgccttt
     2281 ttcagctgca ttcactaatt gttgtgcgta catgatgaat gaatgtgagc tgtgtgtagc
     2341 acctgtaaca acttctacat tagcaggtga gccatcttct tcgcccattg ctttgacgaa
     2401 ttcgtcgttc agagtaggga tgtaagtttt tggttctgta ccgctttttg ctttcatatt
     2461 ttcattatat tctgtatcat cttgttttga tttaccgtct ttgtcgatat aatcaaagtt
     2521 tgattcagtt actttgccgc cagcaactgt catttcgaat tgcacacggt agccgttaga
     2581 gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa tcagcaccat tgtcgatttc
     2641 gattgtatct gtgttaccag cttgagcagc ttggatcaat tgttgtgcgt agttttggaa
     2701 gctttcagat gagtgagtag ctccagttac gacttccaca ccgcttgcgc tttgtgcttt
     2761 taagaattgg tcattcaact caggaatgta tttttctggg tttgtgccag attttgcttc
     2821 catgtttttg ttgtattctg catcttttgt ttttgattcg ccgttttcat tgacgttatc
     2881 atattttgat tcagtgattt tgccgtcttt tacaaccatt tcaaagactg cacggtaacc
     2941 atttgaataa tttttttctt ctaatttata tgtgccgtct tgcaaatctc cgccagctac
     3001 aacttttgca gatgattctg ttgtgctttt tgcagtcgaa gattgtgctg tagatgattc
     3061 agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa gcgcctaaca ctagagctga
     3121 aaatgctaat acggcaaatc cagttacaaa acgttttttc ttcattttaa taccccacca
     3181 ttccatttct agtccacaat ttttctttgt gattcattat acattctaat ctttttagcc
     3241 tataagtcaa ctatttttga taaataaccc atgtaatatt tcaaacgttt tctttcagca
     3301 aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa atccaaaagg agttagacaa
     3361 aagaacagaa catctatata atatatggag attgtgtaat aagtatcaaa ctaaaagaat
     3421 tatcatatat tcattatata gatagtctat aaggagcgga tgatcaatgg caaagcaaaa
     3481 tattgtcgtt gtaggagcag gatatgccgg tgtttcagct acaaaatact tggcaaagaa
     3541 actgaaaaaa gaagatgtag caattactct gatcgatcgt cattcgtacc atactatgat
     3601 gaccgagttg catgaagtag ctggcggacg tgtagaacca gaagcaatcc aatacgatct
     3661 gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc acagacactg taatcggtat
     3721 cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc tatccttttg atcagctgat
     3781 ccttggaatg ggcggagagc cgaatgactt tggtacacct ggtgtcaaag cacacggatt
     3841 caccctttgg tcatttgaag attctgtaag gattcgggaa catattgaga agacagtagc
     3901 aaaagctgca ttggaaccag atgatgaact gcgtaaagcc atgctgactt tcgttgtctg
     3961 tggttctgga tttaccggta ttgaaatgat tggggaactg atcgactgga aaaatcgcct
     4021 agccaaagat tataaattag atccaaacga aatcacactg atggtcgtag aggcaatgcc
     4081 gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa gctgaacgct atttgcgtaa
     4141 gcacaacgtg gaattattat tgaatgcacc aatcgtagaa gtggctgctg atcatatcaa
     4201 attgaaagac ggacgcaaca ttccgactca cacactgatt tggacagctg gtgtcaaagg
     4261 aacttctgac gctgctgact ttggattaga atcagcacgc ggacagcggt tgatcgccaa
     4321 tgaatatatg caggcaaaag gctacgaaga taaaaacatc tatatcattg gtgatttagt
     4381 cttttatgaa gaattcccag gaacaccgac accgcagatc gttcaagctg ctgaacaaac
     4441 aggacacaca gccgcagcga atatcgtggc ttcgatcaaa ggcggagaaa agcataaatt
     4501 taaaggaaac tatcaaggat ttatggtttc tattggtgcc aaatgggggg tagctaacct
     4561 atttgataag atccatttaa gcggattttt agctatcgta atgaaacata tcgtgaattt
     4621 gaaatacttc tttgatattc gttctggtta ttatatgttc caatatttaa tgcatgagtt
     4681 tttccatatc aaagatgatc gcaacgtgac tcgaggacat tcttctcgtt acggcaatgt
     4741 cttatggagt gtgccacttc gtattttcta tggtttagtt tggttcgttg aagcttccaa
     4801 aaaaatcatt gggaatggtg aagtgttcaa accaagtact tggtttggtg aaggatcatg
     4861 gttcactgga aacagtgtgt tcccttggac atgggaatac aatgtaacag atgttgccag
     4921 tggtgcttct gctgctggtg atgcaaccac agctgccagc ggagcagggg caagtgccgg
     4981 agaagcagcg actcaagctg cacattttgg tttgagttat gcatacggtg aagaaccaat
     5041 ggctgtgatt gagaagtcac cagactggtt cacaaagatc atgcaagtga tcatgccaaa
     5101 caatgatgta gcaatgttct tccaaaaatt catggtattc tttgaaattg ccttggcatt
     5161 agcattgatt gctggattat tcacatggct atgcagtgcg acaacgattg tattggtgat
     5221 cacgttctgc ttatcaggca tgttttactg ggtaaacatt tggttcattt ttgttgcttt
     5281 cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa ttccatggct
     5341 acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg gcaagaaata
     5401 aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg agacaaaccg
     5461 tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa gcctccatga
     5521 gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tata
//
LOCUS       DACEND010000139         5538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEND010000139 DACEND010000000
VERSION     DACEND010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.144"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..179
                     /locus_tag="IW746_002338"
     CDS             <1..179
                     /locus_tag="IW746_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1079221.1"
                     /translation="KVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYT
                     SQGKERYVVQGELQ"
     gene            231..2207
                     /locus_tag="IW746_002339"
     CDS             231..2207
                     /locus_tag="IW746_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1079222.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            2220..2972
                     /locus_tag="IW746_002340"
     CDS             2220..2972
                     /locus_tag="IW746_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1079223.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2988..3743
                     /locus_tag="IW746_002341"
     CDS             2988..3743
                     /locus_tag="IW746_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079224.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3756..4016
                     /locus_tag="IW746_002342"
     CDS             3756..4016
                     /locus_tag="IW746_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079225.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            4013..>5538
                     /locus_tag="IW746_002343"
     CDS             4013..>5538
                     /locus_tag="IW746_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1079226.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     "
BASE COUNT         2012 a          919 c         1127 g         1480 t
ORIGIN      
        1 acaaggtaac gcgtaaattt atcgtacctg ggtactttag attaatcgat ggcgttcctg
       61 aagagaactc tttcttgtct ttgccaaaga aaggagagaa acccttatta acgttgttta
      121 cttgtgtcta tacgtcacaa ggaaaagaaa gatacgtggt acagggagag ctacaataac
      181 aaggttttaa tcaacgcgag tcgttgaaat atgaaatgag gagagtaaaa atggcaataa
      241 atcgatatgt aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaagctgct
      301 ttcaggggta tcaagcacat gcagaagatg tcacgcaaaa aacgccacca gagaaggtca
      361 atatcacggt tcataaatta atgtacgatc aaagtacgca attgaatgtt gatattgacg
      421 ggatcaaaaa tgatggttat acgcacgatc agtatcctac aggtgtaacg aaatacaaca
      481 aggcagatta tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga
      541 ctgaagattc tgatctaacc aacgcgaaag tggacgaaat cgtgaaagac gtagaagaca
      601 agggatctaa cagtgagtac gtcaagaatg caaccaataa aattacttca gctgtagatg
      661 aaaatggtga gattactttt gcggatcaac cagcgtacgt gaactcaaaa ggaaatgtgt
      721 atgttattta cgagagtaag tccgcagcag gattagtaac tcaaaaagca aaaccaatgg
      781 tggtcattgc tccaatgact gacaacacag gttcaggttt tttaaaagac attcacttat
      841 atcctaaaaa tattgtttct aagttgagtt ttgaattaac caaattcggt gatgatggta
      901 cagagcaaag caaacaaacc cctcttaaag gcgctaaatt cgaattgtat aaaggagaac
      961 ctggaaaagg aagcaaacta ggcgatcttg tttcggatga tcaaggaaag ctgactgcta
     1021 ctgatttgac gttaggaaaa tactattttg tggaagttcc ttcagaagtc gtagtgggat
     1081 cagataaaga accaacagcc gatcaatatt tattaggcgc agacgctcgc aacgatgcgc
     1141 acaataaatt aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat
     1201 acgtaaacta taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac
     1261 attctttcca agttggtgat gccgttaatt atcaaggaac gattcatatc ccaacagaca
     1321 ttgcaggcgg agcagacggt atcacagtaa acggcgtgaa aagcgaaact tctccttaca
     1381 gtgtatttaa atggggggat acagcaggac aaggactttc ttatgtcgct gctaaagcaa
     1441 atatcaaagt aacaaataaa gatggatcag tggtattaaa agaaaataca gattataaga
     1501 tccaaaattc agaaaatggc tttgtgatcg attttatcgt aaataatggc caagtaagtg
     1561 atactgtagc aagtttacac ggacaagatc ttcaaatgac ttataacatg tatgtaaatg
     1621 acagcgcagc tgttgccaat ccattaacaa atagcgtgga tttcgtttat aacaacaatc
     1681 catttaatca ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcga
     1741 aattcttaaa agtcgatagc ggactatttg gaacaggaat taaggcaact cctcttgaag
     1801 gcgcagaatt tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag
     1861 ataaagatgg tgtcaaagaa gcagtatggg tagacgatgt agcaaatgca gcaatcctaa
     1921 aatcagataa agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg
     1981 aagaaacaaa agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag
     2041 acaaagattc atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg
     2101 taccaatgac tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgcttg
     2161 gggcaggcgc aatttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa
     2221 tgattatcag acaccctaag aaaaagcgaa tcatgggtaa atggatcatt gctttctggt
     2281 tactttcagc tgttggtgtc ttacttttga tgcctgctga agcgagcgtg gcgaaatacc
     2341 aacaaaatca gcagattgcc gcaatcgatc gaacaggaac ggcagcagaa acggattctt
     2401 ctttggatgt ttctaagatt gagttaggcg atcctgttgg tattcttaca attccaagta
     2461 tttctttaaa actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag
     2521 ggattacgga aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcag
     2581 ggcacagtgg actttataaa gataatcttt tcgatgattt gccttcagtt aaaaagggag
     2641 aaaagtttta tatcaaagtt gatggtgagc agcacgctta tcaaatcgat cggattgagg
     2701 aagtgcaaaa agacgaacta caaagaaact ttgtcactta cttagaacca aatcccaatg
     2761 aagatcgagt aacgctaatg acatgtacgc ctaaggggat caacacacac cgtttcctag
     2821 tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa agatgaagaa aataagaaac
     2881 aaaaattatc ttggaaatgg cttttaggaa gtactgtttt tctttcagtc atgatcattg
     2941 gttcgctatt tgtttataaa aagaaaaaat aaaggagggt aaccaacatg agaaatagaa
     3001 aatggctatt tgtttgtgca gccgctcttt tttctttcac tgcctacaca cagttgacgt
     3061 ttgcagacag tcaagaaagc acggatagca cagaacaaac aatccaaacg tcagatagca
     3121 gcacgattca aagcagcgat gaaaccgtcg attcgtcaac agtggattct tcagaagaac
     3181 cgaaaattgc tgaagacgat caaatagtca aacagaccat tcacgttcaa aagattattt
     3241 cttcagaaca attagacaac caggggcatg aaaaacaaga aggagtaaat ggcgcgaagt
     3301 tttctgttta tgatgtatcg gacattttac aaaagatgga tgttaaggat ctcactactg
     3361 accaaattga atcccaatta aaagatcgag tgaaaaaact atccagtgat caattgaagc
     3421 tagtttctaa tggagaaaca aagacaattg atcaacaaac aggtgtcttt gaattctcgg
     3481 tagaagtaca ggcaaatcag aaacaagctt attatattgt caatgaatca agtcctgaaa
     3541 acatttctaa tagcgaggat attttgctat taacacctgt ttctgaaaaa aacggtgaat
     3601 tcttaaagga tgtgtggatt tatcctaaat cagaagcttc gcaacctaaa gaagaagtaa
     3661 aaaaaatcgt ttctactggt gtaaagaaaa acttctttga gaattgttgg gattttgtta
     3721 ctcacttgtt ttctagagat taaggaagaa ataagatgaa actgatcaaa ttattattat
     3781 tcaatctttc ttgtttaacc gcagtctttt tgtactgcgg tttattattt tctcaattat
     3841 ctattttgcc aagctaccaa ctatacctaa tgaggatttt aaacatacca gctgcttcca
     3901 tgccgctaga tattatggaa aagctcatta ggaatccatt atttactacg atcaatttga
     3961 tcgttattgt cggaatttca ctagtcattt ataaaaaaga ggggatgaag ggatgaattt
     4021 taaacactcg tggaccagac ggttaaaata tgtttttccc ataatgatga tgttaggtac
     4081 attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa gttattgtcg atccgtcaaa
     4141 tccaatccag aacgtaaaga ataattttat tattccaaaa gatgttccta atgcaaaaac
     4201 aaatattact gtaaacggag cgtccaaagt ttacaaatat catgttgatg ccaacaaagg
     4261 tggttttaca gacgactacg taggattagt ggagggaaac aaaaatatac gctatccttc
     4321 tttccaaaag gaatacggtg aaacaaacct tgttttagaa ggcgtaacaa ccaacaccaa
     4381 agtcaatatt gactatggaa aaatcggtac gtataacggg aaaaaagtca atatcaagct
     4441 agttctatct aatattcact tatactcgga tacgttgccg tggaatctac tagataacaa
     4501 ctatacaaaa actcattttc gagacgatgg ctataaaaat acgaatggtg caatgagtaa
     4561 atctaaaaaa agaacagtac tttggatctc agacaatctt ttctcaggaa ttgtttacca
     4621 ttcaacacaa atgaatgtgc agctagtagc cacatatgag gacggtagcc ccgttcaatt
     4681 ttcaggagat acatttatca gttttaactc tttgaaccct gcaggaggaa aaagcacgga
     4741 tcttaaagga gaatatgccc actatgacaa aatgaatacg acagattggt atgtcagaca
     4801 agatgcggtt ctttcagaat ttaaatcttt ctacaacaat ctgaacgtgg taggtggcca
     4861 cccaggagga agctctaagc tgacacaagc ggataatgat ttcaataatt tgcatgataa
     4921 attaggagat ccgaagtttg gtcaaggtac tgtatccttt aaaatatctg aagcaaatcc
     4981 gacgtttgtg attggttctt ctaatgttca aacctggttt actttatctt ctgcgacaat
     5041 ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt gataaaaatg gaaataacgt
     5101 gaacgataaa atgttgcaag taggagacac gattcaatat cgaatcaaac agaaggtgaa
     5161 tcgattagga gtggatttac tagcggttta cgatcgattt gagttaatcg ataatttacc
     5221 aaaagaagta gattacgtcg atgcacacgt tgaaagtggt acaaataaaa aattcgatgt
     5281 atctggtgaa gtgacctatg acaaaacaaa acaccaggta aaatatgcag cgaaagctga
     5341 tacattaaaa aatcgaatga agtataacgg cgaaacttac gaattagtga tcaatgtaaa
     5401 agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat cagggaacgt cgatcattaa
     5461 taaagtagaa aaggatacaa atatcattac cgtttatttc cctaaaattc cagtgaaaga
     5521 agtacaacaa aatggaaa
//
LOCUS       DACEND010000140         5519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEND010000140 DACEND010000000
VERSION     DACEND010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.145"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW746_002344"
     CDS             complement(72..542)
                     /locus_tag="IW746_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1079227.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW746_002345"
     CDS             872..2512
                     /locus_tag="IW746_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1079228.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW746_002346"
     CDS             2666..3988
                     /locus_tag="IW746_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1079229.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IW746_002347"
     CDS             complement(4056..4490)
                     /locus_tag="IW746_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079230.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IW746_002348"
     CDS             complement(4555..5391)
                     /locus_tag="IW746_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1079231.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1648 a         1100 c         1007 g         1764 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaa
//
LOCUS       DACEND010000141         5496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEND010000141 DACEND010000000
VERSION     DACEND010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.146"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..2130
                     /locus_tag="IW746_002349"
     CDS             70..2130
                     /locus_tag="IW746_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079232.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2213..2782
                     /locus_tag="IW746_002350"
     CDS             2213..2782
                     /locus_tag="IW746_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079233.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2957..3763)
                     /locus_tag="IW746_002351"
     CDS             complement(2957..3763)
                     /locus_tag="IW746_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079234.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3901..5415
                     /locus_tag="IW746_002352"
     CDS             3901..5415
                     /locus_tag="IW746_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1079235.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1953 a          954 c         1118 g         1471 t
ORIGIN      
        1 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
       61 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      121 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      181 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      241 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      301 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      361 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      421 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      481 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      541 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      601 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      661 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      721 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      781 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      841 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      901 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
      961 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1021 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1081 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1141 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1201 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1261 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1321 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1381 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1441 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1501 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1561 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1621 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg
     1681 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1741 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1801 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1861 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1921 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     1981 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2041 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2101 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2161 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2221 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2281 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2341 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2401 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2461 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2521 gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag ggctcgcaaa
     2581 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2641 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2701 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2761 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2821 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     2881 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     2941 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3001 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3061 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3121 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3181 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3241 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3301 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3361 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3421 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3481 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3541 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3601 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3661 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     3721 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     3781 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     3841 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     3901 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     3961 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4021 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4081 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4141 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4201 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4261 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4321 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4381 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4441 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4501 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4561 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4621 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4681 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     4741 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     4801 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     4861 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     4921 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     4981 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5041 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5101 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5161 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5221 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5281 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5341 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5401 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5461 taataccgag ggtcagctga ttgaacactg tttagt
//
LOCUS       DACEND010000142         5496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEND010000142 DACEND010000000
VERSION     DACEND010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.147"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(167..625)
                     /locus_tag="IW746_002353"
     CDS             complement(167..625)
                     /locus_tag="IW746_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1079236.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(638..1963)
                     /locus_tag="IW746_002354"
     CDS             complement(638..1963)
                     /locus_tag="IW746_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1079237.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1980..3392)
                     /locus_tag="IW746_002355"
     CDS             complement(1980..3392)
                     /locus_tag="IW746_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1079238.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3409..4173)
                     /locus_tag="IW746_002356"
     CDS             complement(3409..4173)
                     /locus_tag="IW746_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1079239.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4277..5152)
                     /locus_tag="IW746_002357"
     CDS             complement(4277..5152)
                     /locus_tag="IW746_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1079240.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5407..5491)
                     /locus_tag="IW746_002358"
     tRNA            complement(5407..5491)
                     /locus_tag="IW746_002358"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5455..5457),aa:Leu,seq:caa)
BASE COUNT         1688 a         1153 c          807 g         1848 t
ORIGIN      
        1 tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt
       61 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
      121 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      181 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      241 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      301 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      361 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      421 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      481 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      541 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      601 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      661 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      721 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      781 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      841 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      901 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      961 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
     1021 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1081 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1141 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1201 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1261 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1321 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1381 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1441 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1501 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1561 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1621 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1681 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcgt cctgtaaaag
     1741 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1801 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1861 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1921 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1981 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     2041 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2101 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2161 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2221 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2281 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2341 acgtaataca aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2401 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2461 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2521 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2581 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2641 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2701 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2761 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2821 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2881 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2941 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     3001 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3061 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3121 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3181 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3241 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3301 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3361 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3421 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3481 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3541 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3601 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3661 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3721 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3781 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3841 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3901 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3961 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     4021 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4081 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4141 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4201 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4261 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4321 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4381 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4441 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4501 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4561 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4621 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4681 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4741 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4801 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4861 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4921 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4981 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     5041 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5101 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5161 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5221 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5281 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5341 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5401 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5461 ccagcgcgtc tgccaattcc gccacgccgg caaatt
//
LOCUS       DACEND010000143         5440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEND010000143 DACEND010000000
VERSION     DACEND010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.148"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..396)
                     /locus_tag="IW746_002359"
     CDS             complement(40..396)
                     /locus_tag="IW746_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079241.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(694..1668)
                     /gene="sgrA"
                     /locus_tag="IW746_002360"
     CDS             complement(694..1668)
                     /gene="sgrA"
                     /locus_tag="IW746_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1079242.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1864..2703)
                     /locus_tag="IW746_002361"
     CDS             complement(1864..2703)
                     /locus_tag="IW746_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1079243.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2888..3775
                     /locus_tag="IW746_002362"
     CDS             2888..3775
                     /locus_tag="IW746_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1079244.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4359..5054)
                     /locus_tag="IW746_002363"
     CDS             complement(4359..5054)
                     /locus_tag="IW746_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1079245.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(5038..5436)
                     /locus_tag="IW746_002364"
     CDS             complement(5038..5436)
                     /locus_tag="IW746_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1079246.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1735 a         1093 c          795 g         1817 t
ORIGIN      
        1 tattaagttc tcttaatttt ctttgaatcg tacgcaaact tatataattt tttgactaag
       61 attttctaag gattgataag tacctttgta aagtgaatcc agtaaaatat aaccaatcgt
      121 tccctctaaa agtatagaat aaactttact cagtaaaaac tgagaagaaa actcgacttt
      181 tatcatcttg ttttctgaaa gaaaagataa tttatattct ggataccttt cgcaaaacga
      241 ttctgcttgt tgaatcaccg aattcatctt agccagggtg tagtttagat ctaaagcgat
      301 atccttcacc acatataatt tctcgtctct ttcataacaa tagttcaaaa attgatatac
      361 ttgcctttca ttgatatcaa taaattcttc aaacaaagta catactcctt tttatctatt
      421 atttagactt attccgacag ctacatgaat aacgtacaaa tagattcgtt actgattttt
      481 cagagatact accatgcgca gtgtgcattg tcaattattc ttgctcttat tttccatttg
      541 tgttcctgtt atttcctgtt tctcaaatat gttagtaaac aaaaatagat ctggcaaagc
      601 tacaacttcg ccaggcaata cagcactctc gaatgaaata ttcactcaaa aatctatcgt
      661 tttattcaat tttagatctg ttgcttttat tctttatttc actcgatgga agagagaacg
      721 aaaatagctt gctcctacaa tcaaaatgag taacacaccc atccatgtaa ttagcttgtt
      781 caaggttcta ctaccagttt ctggtaaatc gctttttcta ttatctgttt ctttcgcttt
      841 ttcttgatga tcaattccac gagaaacaaa atcaggctgt tttagcacac tttttaggtt
      901 gctccctgca tttctctttt gttccttagt tggtatgata ggtaccaaat caagttcatt
      961 atttactttt cctgtgctgc tttcgctgct cgtgcttcct gtgctactct cactactcat
     1021 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc
     1081 tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgt tttctgtgct
     1141 actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg tactgctttc
     1201 actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc ttggtttcaa
     1261 attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt tatctttact
     1321 cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa taccagcggt
     1381 ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc cattaccatc
     1441 ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt ttggtttgac
     1501 tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa
     1561 tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca ttgctgcaat
     1621 tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt ctatcactct
     1681 ccatataatt accttctatt tgataaagga attatataaa atgaaaatga tgagaacacg
     1741 acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt
     1801 ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg ctttcttcat
     1861 cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg catagagtaa
     1921 caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt cagccccaaa
     1981 gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa aaccttgttc
     2041 ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc aagtaatatg
     2101 agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga aaaagcaacc
     2161 tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag
     2221 agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa gcatctgatg
     2281 ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa ataaaataaa
     2341 actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa
     2401 atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac ctcccgctaa
     2461 atacccccag tcttttcctc gaacagaaac accataatct tctattcttt ttccttcgac
     2521 cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat
     2581 tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat tgacaagaat
     2641 tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa taatcctgat
     2701 catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg tctcgtgaaa
     2761 actatgtaaa tgttctccat catagagaga atagattttt acgaactaag aatatctaca
     2821 gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg tttttaataa
     2881 ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt cagcatgtgc
     2941 aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg
     3001 aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata atcaaacact
     3061 attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag agatttcttc
     3121 aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa actacaagga
     3181 acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta aacagatgct
     3241 ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc tagacactgc
     3301 ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga ctaaagaaga
     3361 agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc ttgttcaagc
     3421 ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg attcaaccaa
     3481 tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc
     3541 tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc tagtaaaaat
     3601 ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc tagaaaaaat
     3661 tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc gtaaagtgat
     3721 gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca aataaacaaa
     3781 tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat tccttcttat
     3841 ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt tgtaccaaaa
     3901 caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt ttatttctat
     3961 cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt ttcacttgcc
     4021 gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta ttttaaaaaa
     4081 gactaaaaat atcccttctg gacttgttcc tcttattctg ttatactaga gaaacacgaa
     4141 agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa actggcgtaa
     4201 cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat gagaaataag
     4261 taatagtcac ccaaacttta aattaatgaa tattgaatcg tcagatagcc tcctcgagtt
     4321 ttcgagaagg ctatcgttta ttttatggag aaattgattc agaagtaatc agcgaccttt
     4381 tttagttcac caatcgtttc tgcttggaaa gtttgcaccc aatcttcttg gaagcccctc
     4441 actgctttat caatcgaggg catctctaat acttgatcta gtagctcttt acctaacgtt
     4501 atcgcatgag ctccagatcg aatggctgta gtgacttgtt cacaattttt gaaactagct
     4561 gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga atcaatcact
     4621 ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat ataatctgct
     4681 ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc ggtaatcaaa
     4741 taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac cggcaccttg
     4801 ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat catttcttct
     4861 tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt tttgatttct
     4921 ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg atttgttgtt
     4981 acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt tgctgtatca
     5041 agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt taatgattgt
     5101 cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg gaagttccgg
     5161 ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat tagtttggac
     5221 aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct tcatatcaca
     5281 agttatttct tttcttacct gattcaattc aatcttataa caataatctg ttaatgtttc
     5341 cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt ctgcttctat
     5401 accaacatct gttacaaccg tttcaaagat cgtcatctct
//
LOCUS       DACEND010000144         5356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEND010000144 DACEND010000000
VERSION     DACEND010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.149"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2068
                     /locus_tag="IW746_002365"
     CDS             <1..2068
                     /locus_tag="IW746_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1079247.1"
                     /translation="KKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDESKDLMIEAAT
                     GMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQPIQPVIIKS
                     YRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTNLNHSFFADI
                     AHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEPLLPASPFLL
                     IDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEHEALRWLSIY
                     REELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLESGTAIDHLQL
                     FYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMDQWLEDWRDH
                     LIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGPNRHYLSKRL
                     GIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTGLIEQEDRPI
                     LVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEKSVLFGADSF
                     WEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESVPKAALKLRQ
                     ALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEIYQDIHEFLK
                     KNEEGS"
     gene            2115..2642
                     /locus_tag="IW746_002366"
     CDS             2115..2642
                     /locus_tag="IW746_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1079248.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            2663..3853
                     /locus_tag="IW746_002367"
     CDS             2663..3853
                     /locus_tag="IW746_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1079249.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            3893..5191
                     /gene="asnS"
                     /locus_tag="IW746_002368"
     CDS             3893..5191
                     /gene="asnS"
                     /locus_tag="IW746_002368"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1079250.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         1785 a          987 c         1181 g         1403 t
ORIGIN      
        1 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
       61 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
      121 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
      181 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
      241 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
      301 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
      361 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
      421 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
      481 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
      541 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
      601 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
      661 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
      721 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
      781 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
      841 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
      901 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
      961 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     1021 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     1081 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     1141 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     1201 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     1261 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     1321 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     1381 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     1441 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     1501 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     1561 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     1621 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     1681 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     1741 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     1801 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     1861 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     1921 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     1981 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     2041 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     2101 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     2161 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     2221 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     2281 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     2341 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     2401 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     2461 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     2521 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     2581 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     2641 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     2701 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     2761 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     2821 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     2881 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     2941 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     3001 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     3061 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     3121 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     3181 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     3241 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     3301 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     3361 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     3421 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     3481 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     3541 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     3601 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     3661 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     3721 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     3781 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     3841 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     3901 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     3961 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     4021 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     4081 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     4141 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     4201 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     4261 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     4321 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     4381 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     4441 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     4501 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     4561 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     4621 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     4681 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     4741 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     4801 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     4861 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     4921 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     4981 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     5041 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     5101 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     5161 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     5221 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     5281 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     5341 tcgagtatta agaaaa
//
LOCUS       DACEND010000145         5109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEND010000145 DACEND010000000
VERSION     DACEND010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.151"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..191)
                     /locus_tag="IW746_002369"
     CDS             complement(<1..191)
                     /locus_tag="IW746_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1079251.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICA"
     gene            384..1184
                     /locus_tag="IW746_002370"
     CDS             384..1184
                     /locus_tag="IW746_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1079252.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1203..3071
                     /locus_tag="IW746_002371"
     CDS             1203..3071
                     /locus_tag="IW746_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1079253.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3064..3555
                     /locus_tag="IW746_002372"
     CDS             3064..3555
                     /locus_tag="IW746_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1079254.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3543..4097
                     /gene="srlA"
                     /locus_tag="IW746_002373"
     CDS             3543..4097
                     /gene="srlA"
                     /locus_tag="IW746_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1079255.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4115..>5109
                     /locus_tag="IW746_002374"
     CDS             4115..>5109
                     /locus_tag="IW746_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1079256.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1789 a          815 c         1054 g         1451 t
ORIGIN      
        1 atcgcacaaa tacctacgac gatataaatg atcttcgcaa aaatagttgt tgaaccacca
       61 gcaatcatag ctactaaatc aagttcgaac aagcctacca acagccagtt caaccctccc
      121 acaatcaata aagttaatgc aatagcatct aagttttcat tagaattctc ctttcttaaa
      181 tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc aggaatttta
      241 gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc
      301 tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag
      361 aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa
      421 ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga
      481 atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat
      541 ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga
      601 aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc
      661 taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga
      721 tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg
      781 tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag
      841 aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg
      901 caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag
      961 aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg
     1021 tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta
     1081 agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca agttatatca
     1141 ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc
     1201 acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag
     1261 tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata
     1321 ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt
     1381 ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa
     1441 cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg
     1501 aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa
     1561 cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta
     1621 caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt
     1681 tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa
     1741 aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt
     1801 taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc
     1861 ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc
     1921 agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc
     1981 ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct
     2041 tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt
     2101 ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc
     2161 gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc
     2221 aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag
     2281 tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt
     2341 tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc
     2401 aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac
     2461 aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa
     2521 atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa
     2581 gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt
     2641 tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc
     2701 ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat
     2761 atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat
     2821 ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag
     2881 gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg
     2941 aattatttga gctatacgat atcctatttg aactcataaa aatgggaata gatgagttta
     3001 ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac
     3061 ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca
     3121 gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa
     3181 ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta
     3241 gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg
     3301 gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac
     3361 catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa
     3421 gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt
     3481 gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa
     3541 cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag
     3601 aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa
     3661 tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag
     3721 cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa
     3781 atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg
     3841 ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag
     3901 aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg
     3961 agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa
     4021 ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag
     4081 agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag
     4141 aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt
     4201 catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac
     4261 aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc
     4321 aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac
     4381 catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca ctgaagatat
     4441 ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa
     4501 agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat
     4561 aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa
     4621 agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt
     4681 gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg
     4741 taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat
     4801 tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag
     4861 ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt
     4921 agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg
     4981 tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta
     5041 ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt
     5101 atatagctg
//
LOCUS       DACEND010000146         5036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEND010000146 DACEND010000000
VERSION     DACEND010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.152"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(670..1482)
                     /locus_tag="IW746_002375"
     CDS             complement(670..1482)
                     /locus_tag="IW746_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079257.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1645..1752
                     /locus_tag="IW746_002376"
     CDS             1645..1752
                     /locus_tag="IW746_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079258.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1810..2877
                     /locus_tag="IW746_002377"
     CDS             1810..2877
                     /locus_tag="IW746_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1079259.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            3019..3222
                     /locus_tag="IW746_002378"
     CDS             3019..3222
                     /locus_tag="IW746_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1079260.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            3282..4484
                     /locus_tag="IW746_002379"
     CDS             3282..4484
                     /locus_tag="IW746_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1079261.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1676 a          789 c         1045 g         1526 t
ORIGIN      
        1 gaatcatcac tgttgtatct tattaccttt tctgtttttc ttattagaga aaaataattt
       61 gagaggtaga tagtttttat tggaggaagt aattatgtat atttttgaaa caaaaatttt
      121 tcattgcaat aattgcaaca aagaaatttc atcgggggaa gaggtttgga caaaaatgga
      181 aatttccgcc aaaagtaact gctcctcaac tgaaatcaat taaagaacta gaatttgaga
      241 atgcactggt aatttgtatg acatgtactg atgaagtatt tcgaaaacat ttttaaaggt
      301 tgagatgtat atagatttaa ccaatcattt atctctctcg gaaaaggaat attaactaac
      361 cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat ggcttatttc gcgctgatta
      421 tgagaatatc agtctgtttg attaattttt agggttcaaa atacagagac aaactccagg
      481 tcgggtttcg ccacaagcga attacccgat ctggagtttt gtctattgca ggaaggcgta
      541 gccggactgc aggaataaag cccccggttc taacaggggg ctaacaaaga acaggaaact
      601 gtgacaacta cccattgggg agtgtcctag cagtattttc tttgtcacgt gcttatcaat
      661 tttgtcacct cattttgatg atggatacgc tgcatttaac aggcgttctt tctcgacttc
      721 catcacgaat ggtagataaa gatattgctg ttctagtaga tattgattgt cctctgtgta
      781 ctgataagaa gcggtttctt ctgaatagac caacggggca attcgcacat cgctggtcaa
      841 ttgattaatt gagtatagaa ggatggcttt ctcacctagt tccttgggta aatgtttccc
      901 aaaataggaa taggattttt gatttgctaa atagacgagt atgcgatcca cactaaagtt
      961 ggagaaggta aagggcatca tgtgcagaat atgattttga ttatcatgtt gcaaatagag
     1021 ctgggcttcc ttaccaaaat tagttgaacc aagataaagc tgcttttttt catcgataca
     1081 ttggaaagta aactggtctt gttcagttgg tggaatgaga tttgtaataa aaaattcaat
     1141 cattcgatcc atttcttttt cactaacaag gtcagcgtgt ttgatttcgt ctactatgag
     1201 accttcaatc ttaggatcaa gcttacctgc ctttttttgg agcatttcta aaatgtattg
     1261 attctcttga tcaccataca aaagatattc attggtgaca tgacctatct ctgcgatttg
     1321 gtgaagggtt tcggtttttg gaaggttgat cccacgttcc cagttattga ctgtacttcg
     1381 cggtaaatca ccaattagtt tgccaaattt ctccatactc aactttaatt cttggcgaat
     1441 ctgccgaatt cgattgccga ctgcttgctt gtctatctcc atgaaaactc ctcctcagga
     1501 taagttctta aatttagtat acgtgtgtac ttaaaaaaag tcaaaacata ttgacttaaa
     1561 ataaatacag gcgtacaata aaaatgtact taaaataagt tcaataggtt ctttgacaag
     1621 taaatagcag aaggaggttt gtcgatgaac caagttaatc aacatgatct cggtgaagcc
     1681 attcgagttt ctcgagaaga aagggggtgg acacaacgat atttagcaga aaaagtgggg
     1741 atttcgagat agttattatc gaaagtagaa aaaggaacga gacaattaag tgaagaaaaa
     1801 ttgaatcttg tgttggatag tttgcaagaa gaggtcgttc cagttaaccg tgttttgatt
     1861 gattatctaa ccattcactt tttctcgaat caacatttaa agctaattga agagattatt
     1921 gaaatgccca ttgaacgttt tgaggaactg gattatgcgc caaagggcta tattggccaa
     1981 tacgtgtgga atcaagtaat cacgattcgg tattccattg acgatgcggt taaaggaact
     2041 gtgatggagt tttcaggtca aggctgtaaa cacttagcaa gatggctaaa aacacgaaaa
     2101 acaaattggc aacagttttt tcgtaaggta ttagattatc aggggaactt tacacggatt
     2161 gattttactt tagatgattt tgtggggagt ctcagcattc cagaacttaa gcataaggta
     2221 atactaggcc atgtgtggac cacttttcaa gtgagtgaat ctcatggcgg tacggatatc
     2281 ataaacaatg aatcaaatgg tgagaccttg tatttaggct caaagaagag tcagtgtcgt
     2341 ttttgctttt atcagaagga ccatgaacaa cggaagcgac gaggaattcc tttagaagag
     2401 gcaaaagtaa agaatcgctt tgaactacgg tatcgaaaag agaaagcgca aagcttggca
     2461 aagattattt caaaaaccca tgatttaacc aaactgttct ttgagttact taatggagca
     2521 atttgttttt atgaccgtga tccaaatgat ctaggtgcaa aagttgatgc caaatgggca
     2581 gcctttatta gcaattatgg cgcaatcacc atttctttag atacaattcc tcaaagtttt
     2641 gagaaaacta tgagttggtt aattcatggt gtttcaccat cattagtttt tattgcacag
     2701 gtcgatcaaa cgtttcattc ttccttgttg cctttgatta ttgaacaagg agaaatgaat
     2761 ccaagacaga tcaaacttct ccaaaatatg aaggcagacc cggagtatta tcaggaggag
     2821 gttgagtatt atgaacgaaa attacaaagt atatcaaatg agaaaacaag ctactaaaaa
     2881 agcaaagtac tgcttacaca acaagttgtg caacgtactc cgcctcgaca agacgagtat
     2941 aacacaacca tcgaatgaga aacaatagat tataaagagg attattttaa aaggagtaaa
     3001 gaatcaggag gtgagagaat ggaaacaaaa tgtgatttca tggtaaatcg tgcaattttg
     3061 atagagatgg gctttaaacc tagtcaagct gcacgaatga ttaaagagag caaggcatat
     3121 cttgcaagga ttgaaggtat tgatttttat aataatcgcc aagttggtgt ggtgccatcc
     3181 cgagtaattg aacagctttt tcacattcaa gttgcggaat aaacagtaca agcgcggtac
     3241 tattaaagta gcttttctca agtacaggga ggaatggaac gatgccaaaa attaaaaata
     3301 tttaccaaga taaaaagaca aaaaaatggt tcttccgagc gtatctaggt atagatgaaa
     3361 atggaagaaa ggttcagaag actaaacgag gttatgcttc acaaaaagat gccaaaattg
     3421 cttatgacaa gtacatggaa acacatgatt ttaacaagac aattcctaat caactgtgtt
     3481 ctaccaatca aatgactttc gaagagtttt acaaggatag gtttgtaaaa tggtacgaga
     3541 gacaggtcaa aagtcaaaca tatgagaatg cgcaattcat tttcgaaaag cgtatgcagt
     3601 ttttctatca ttttcgtgtt cgagatatta caagtcacga tattgaggat tggttatttg
     3661 aactaagcca gacagagtca cgtaattcac ggaaaaaagt taatggcgaa acgttatcaa
     3721 aatcttatat caatcggatt cgagggcacc taaaaattgt attggatcgg gctgttaaag
     3781 agggggtgat tgctaaaaat cccgttgaag atgtttcttc gcttccttct gagaataaga
     3841 aagttgagtt ttgggaacac agtgagtttc aaaaagtgat gaatgaattg aaggatgata
     3901 agattcaaac ccaccatcgg aagattgtgt atgaaatgct cttttatact ggcattcgga
     3961 ttggagaact tgaagctttg tcttggtcga atgtagattt tgagaaaaac acgataacga
     4021 ttgagaagac attgatttat aatacaaaag atgattggta cttttcgact cccaaaacta
     4081 aatgtgctta tcgaacgatt gggttgggga agaagctttc cttaaaactc agacagtggt
     4141 atgacttgca aaaacgtatt ggaaattttg aatatgttgc tcagttggac ggaacattta
     4201 cgccacctta ttcttttgcg aactggttaa aagaagctgc taaaaaagcg ggtgtcaaac
     4261 cgattaagct tcacgcgcta cgacattctc atgttgcatt tctaattgag cagaatattc
     4321 aaccgctatc tattcaagaa cgattgggcc atgcgaatat tcaaatcact ttaggaactt
     4381 acggtcactt atatgcaaag tcagatcaac aagtggtaag tgcgattgat aatgttcaag
     4441 agggcgctat tctttcaaaa aatgacaatt tattaccagt ctgagttttc cacggttcta
     4501 ccataagaca aataaatgct tttgcaccag tgttttcagt cgaattgctc ctgttcaaga
     4561 tgtatcggaa atagcgagaa aataagcaaa tttgcataac tataataaaa aggacttagt
     4621 tttgatggga aatttttcaa aattaagtcc ttttttatgt ggttgaaacg ggtatgatca
     4681 ttttaaggaa gtgatctaag atgaaaggac gactgaacaa ttatcatatt agaaaagctt
     4741 tattgtattc cacaaacaaa ttactaagaa taacaaatta gtttttgttt gttcttattc
     4801 cctattgact tttctttcaa acatgctatt ctaattttat tctaatagta tccgatgaga
     4861 aggaaagtag acagaaaatg gttcaagaga ggctttggtt ggtgtaaaaa gctagaagtt
     4921 gactgtcgaa aatggcctta gaggattttt tgtcgaaagg taggcaaaaa cgaacagtct
     4981 ttcgttacca agaccactga ttttattgtc aggctgagag aaagaatgtt ttttct
//
LOCUS       DACEND010000147         5027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEND010000147 DACEND010000000
VERSION     DACEND010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.153"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1933)
                     /gene="pepF"
                     /locus_tag="IW746_002380"
     CDS             complement(125..1933)
                     /gene="pepF"
                     /locus_tag="IW746_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1079262.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2109..3107)
                     /locus_tag="IW746_002381"
     CDS             complement(2109..3107)
                     /locus_tag="IW746_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1079263.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3333..3986)
                     /locus_tag="IW746_002382"
     CDS             complement(3333..3986)
                     /locus_tag="IW746_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1079264.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4318..4716)
                     /gene="spxA"
                     /locus_tag="IW746_002383"
     CDS             complement(4318..4716)
                     /gene="spxA"
                     /locus_tag="IW746_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1079265.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1497 a         1038 c          771 g         1721 t
ORIGIN      
        1 aagatgttgg tgaagttcac caacatcaaa aagtatagag ccttaaaaaa atacgtcgac
       61 tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa
      121 agccttataa acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca
      181 ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca
      241 caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt
      301 cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga
      361 aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt
      421 cttcttcgac tgctggacca taatattttg cattcaattt accataacta tcacttaggt
      481 attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag
      541 cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat
      601 aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat
      661 tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt
      721 tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct
      781 gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag
      841 aataagcacc gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg
      901 ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt
      961 cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat
     1021 catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta
     1081 acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat
     1141 gattattgct caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat
     1201 tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa
     1261 cactgtacaa ccccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact
     1321 gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa
     1381 aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac
     1441 ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta
     1501 tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa
     1561 tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt
     1621 tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt
     1681 gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata
     1741 cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt
     1801 cagattgttt taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg
     1861 aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact
     1921 gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc
     1981 atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc
     2041 catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt
     2101 tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag
     2161 accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa
     2221 agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga
     2281 taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat
     2341 gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc
     2401 ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct
     2461 atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta
     2521 ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata
     2581 tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt
     2641 tttctattat agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa
     2701 gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca
     2761 ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa
     2821 atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac
     2881 tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca
     2941 ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc
     3001 tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca
     3061 tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg
     3121 aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct
     3181 gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg
     3241 cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa
     3301 tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg
     3361 atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg
     3421 ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga
     3481 ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc
     3541 caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa
     3601 gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga
     3661 tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa
     3721 tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg
     3781 tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata
     3841 tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa
     3901 tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta
     3961 ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata
     4021 tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga
     4081 atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt
     4141 ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg
     4201 caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca
     4261 aaaaaacttg gaaaaactat ctttgtaata gtcttttcca agtttcgtat tatccttcta
     4321 taatccagcc atcaattgtg cttgacgtaa ttctaattgt cgtacatcgc gcggtaggaa
     4381 ccgacgaatt tcatcttcat taaaaccaac ttgcagacgt ttatcatcaa tcatgattgg
     4441 acggcgaagc aatccaggat tgttttgcac aagttccaat aattcttgta acggcagatc
     4501 atctaaatcc atgttcagtt tttggaatac tttggaacgt gtcgagatga tttcttctgt
     4561 tccgtcttct gtcatttgta aaatcgcttt taattcagaa ctgttcaatg gttctgaaaa
     4621 aatattacgt tcaacaaatg gaatctgatg ttcttgcaac cacgcacgag cctttctgca
     4681 ggaagtacaa cttggggaag tatataatgt taacaacgtg caccactcct tttcgtgata
     4741 agaatcactt ttttgtaaca gaagatcata ataatctccc atctgtttct attatacata
     4801 atcaactttt aaatagctac ctttttttca taattttttt aaaaaaatga aagttatcta
     4861 aggttaatat acagtaaaac cagcattctc gcttttttta tcatttaaaa attagagttt
     4921 tcaaatctaa ttcaaatttt ctaaacatat gaaacagcgt ataaaactgt actattattt
     4981 tatctcaaaa gctaaatgaa accgatttct taagattttt tatgttt
//
LOCUS       DACEND010000148         4837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEND010000148 DACEND010000000
VERSION     DACEND010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.154"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(103..2007)
                     /locus_tag="IW746_002384"
     CDS             complement(103..2007)
                     /locus_tag="IW746_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1079266.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            complement(2205..2438)
                     /locus_tag="IW746_002385"
     CDS             complement(2205..2438)
                     /locus_tag="IW746_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1079267.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2585..3439)
                     /locus_tag="IW746_002386"
     CDS             complement(2585..3439)
                     /locus_tag="IW746_002386"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1079268.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3424..3762)
                     /locus_tag="IW746_002387"
     CDS             complement(3424..3762)
                     /locus_tag="IW746_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079269.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3990..4607)
                     /locus_tag="IW746_002388"
     CDS             complement(3990..4607)
                     /locus_tag="IW746_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1079270.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
BASE COUNT         1375 a         1136 c          831 g         1495 t
ORIGIN      
        1 tcaagaactc aaaaaagcgg aaaatcccag atttaaaaat ctgagtttcc gcttttgtca
       61 tttcaaggat ggactattgt ctcagcctct tctaatttat tcttaagata tttttacttt
      121 ctctgtaggt gctgatactt ctttttgttc tttccgacct ttcaacttat acttcattaa
      181 tctcatgcca ttgaaaatta ccaccaagat actaccttca tgaaccaaca ttccgataga
      241 catattcatc cattcgctga agaagacgct ggtcaacaag accaacacta ctccaactga
      301 aatcacgata ttttgtttca tattatttgc ggtggctttt actaatccta atgcgtgagg
      361 caagttgctg aagttcgagt tcattaaaac gacatcggat gtttcaatcg ctacgtccgt
      421 tccgcttccc attgcgattc caatgtctgc taaagctaag gacggactat cgttaacgcc
      481 atctccaaca aaggctacaa tttgaccacg ttgttgaagt tttccgatat aagccgattt
      541 gtcttccggc aacatgtagc cgtgagcttc ggtcaaacca agttcgccgg cgaccacatc
      601 aacggttcct tggttatctc ctgaaagaac gactagattt ttcacgccca agtcttttaa
      661 ttcctgcaaa ttagctttca cacccggacg gacttgatcg cgaatgccca tcagtacttt
      721 taattcaccg tcgaccgctg tcaaaacgag agagttccct tgttgttcaa accgtttgac
      781 atctttttgc acttttttgc tgagagtgac attttctttt tccatcaagg ccacattccc
      841 aacagccact ctatgtccag ctacacttga aacgattccg ccgcctttca caacttcagt
      901 tccttcaaca ggataaaagt ttgtttctcc aatttgattt aagaccgctt ttgctaatgg
      961 atgatctgat tcccgttcca cactggccag atagccaagc gtttcagcag gatctttttc
     1021 atacagttca gtcgctgcaa cggttgggtt tccgacagtt aaagtacctg tcttatcaaa
     1081 tacaattgta tccacattac tgaagtcttg aatgacttca ctccctttta aaagaacacc
     1141 gttacgagca ccattcccaa taccggcaac gtttgagaca ggcactccga taactaatgc
     1201 gcctgggcaa cctagaacca agatggtgat tgctaactca atattttgac tgaacgccca
     1261 tacaacaatt gccagaacca agacagctgg tgtgtagtat ttagaaaagc gatcaatgaa
     1321 ccgttccgct tcggatttag aatcttgtgc ttcttccacg agttcaataa tttttccaaa
     1381 tgtggtgtcc tcaccaactc ggtcagctct gatttgaatc gttccgtttt ctaaaatggt
     1441 tcctgcaaaa acttctgcat cagctagctt gttgaccgga atagcttcac ctgtaatgct
     1501 agcttcattg atatgccctt cacccgtaag gactatgcca tctactggaa ctttcgcacc
     1561 cgttttaacg agcaagatat ccccttcatc tatatcatcc acttctactt cttcaaattc
     1621 gccattatcc atttgtttca aggcgctttc gggtgccatt tcagtcaatt ctttgatagc
     1681 agatcgcgtt tggttcaatg ttcgttgttc caagtagtgt ccgaataaga ataagaacgt
     1741 gacaatagcc gattcttcat aattttgaat aaaaagcgca ccaatcgctg caatactgac
     1801 taagacatca atactgatga ctttgacttt cattgcttga aacgcttgaa tggcaattgg
     1861 cgtaatccca aagacagagg cgatgatcaa agaccactct gaaagtccta cgttttctaa
     1921 aacaaagtga ctgaagaaac cgagtacgat taacaatcca ctgatgacgg tgataacatt
     1981 tttcttgctt aatatatatt gttgcatgct ttttccctcc aaatttaatt tctcttttat
     2041 tgataaacac agtataagat aattgcctaa gtaattaatt gactgacatc aagtttagaa
     2101 agttctcttt tatcgtttct aagcacctcg aatcggattg caacaaaaaa agcaatcctg
     2161 gaaaaatagc ttttcttcca gaattgccta gttcatttaa tctgttaaac ggccttgact
     2221 tttgctttaa gaaccggata gcctacgttt tcgatagctt tttgaatctc ttcaatcgaa
     2281 gtgacagctg aatcaaaatt ggttttgact ttgctggagt tgaataatac tttaatgctg
     2341 tctttatcaa taccgttaac tgatttcacg gcaccttcaa ttttttgcat acaacttgga
     2401 caagacaacg tttctaattg caatatagct ttttccataa ttcctatctc ctttatgtgt
     2461 ttttatttta tcggcagcag attaatgaac actgcctttt cctctttaca actgtagtat
     2521 agctcacttt tagcaaacga aaattgacgc atatcaagtt ttgcacttct ttttatcttt
     2581 ttgctcaatt caccatctta atagtctttt tctgcccctt attgccatca gaataataag
     2641 aaattggtgg ataatcttgt ctgcgtataa accatactac aatcgcgcca ataaacaggg
     2701 ccagagacaa ggcttgcgaa acgcggatcc actcgccaat ccataaactg tccgttcgca
     2761 taccttcaat gaagaaccga cccactgagt accacaaaac gtaactcaaa gcgacttctc
     2821 cttgacgcaa aagctgtttt cggttccgta tagtgacgat gaggacaaag cccaacaaac
     2881 tccacaatga ctcgtataaa aaagtcggat ggtagtaggt accattcata ttcatttgtt
     2941 caatgataaa ttcaggtaaa taaagatttt ccaagaattg acgagtaacc ggcccaccat
     3001 gagcttcctg attcataaaa ttgccccaac gaccaatgga ctgggctaac aatacattgg
     3061 gcgctaaaat atcaagaacg agtgccaaag aagtaccttt tttctttgca taccaatata
     3121 gcgtaccccc tcctgcaatc aatcccccat aaatcgcaat gccgccattc caaatcgcaa
     3181 ggatttgtcc tggattttgc agataataac caagttcaaa taacacataa taaagccgag
     3241 cgccgaggag tccaatcgga attgcccata aggccatatc gataatggta tcctcttcca
     3301 gtcctttttt ctttgcttcc ttggtgctca actggagagc cgctaatatg cccgctgcaa
     3361 taatgactcc ataccaatga atcacaacag aaccaatctc caccacatgg ggattcaaaa
     3421 cgcctaatac gaatcccatt ctatttgctc catttcttca atttttcgcg tcattctagt
     3481 catggtgatg ctgaacaaga ttccggctaa gctccatgga aaccaaacag tgaatacact
     3541 tttatctccc tggtcactcg cttcgtaata cgccactttt ccgtgtccga ttacgccggc
     3601 tccttttaca acttcctctt ctttttccat agctaaccta tctgcctcaa caacgttaga
     3661 cgtttttgct tcgccgccaa tcgtgttctt ggtgaaaggg acgacaaaga atgcccccgc
     3721 tgctgacaac gtgatggcaa ctatcaccca aaaacaatgc acattcaccc tacttatttt
     3781 tacgttttgt tcgacaaccc tatcttaaag aaaaataaaa caaaaaaact tgagggttat
     3841 catgtttgtg cttttatttt tccaatgaat gaaggtcgtt ctgtcttaaa aaagccaaaa
     3901 acggacagat ggaagcaaaa gcactgagtt ctgtcccgtt gttaatgaag agcgacagaa
     3961 caccaacgtg tagaaaaaag tgtgtccgat tattcgctgt aaagcaataa atcatctaaa
     4021 tcctgaatct tgatcctttt cccagacagc tgttgaatca atccttcgtc ctccaattct
     4081 ccaaatttgc gactgatggt ctcaggtgtc gttcctaaat aggaagcaat gtcctttttt
     4141 gccatcggca gggtgatggt gggagagttg cccatttcag gttccacatt ttctgccaaa
     4201 aacaaaacta aacgggtaat gacactctcc acggctactt gtgtcgtttg acgttctgaa
     4261 ctttctaacc gtttagacat ttccgccaat agttttaaag aaatagctgg gtattgttcc
     4321 agaaaatctt ggacatcttt ttgctgaatc atacagacaa cggtatctcg ggttgcttcg
     4381 gcatattcct cgtgtactgc atccgggttg aacaaggtcc attctccggt gaaatcacca
     4441 gggttcaaga tccgcactag ctgctctttg ccagagtctg ccaatcgata aatacggatt
     4501 ttcccacgat tgataatata taaagtatcc tctttttctt gtgaacgaaa cagaaactct
     4561 cctttttgat actgtttcgt gtgggctttt tcagcaatgt aattcatcgc gctatcctct
     4621 aagtgattga atattggaac caagcggata caggctgcgt ggtcgccaat ttcatgatgg
     4681 gtatgtttag tcaattcatt ctcccccttt atttattttg tttatattcg ctgcttttat
     4741 tatataagtc tcttactaca aaaaaattga tgcacatcaa gaaaatggaa aaaggactgg
     4801 aaagaattcc aatccctaaa tcagcttcta cttttac
//
LOCUS       DACEND010000149         4743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEND010000149 DACEND010000000
VERSION     DACEND010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.155"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(152..514)
                     /locus_tag="IW746_002389"
     CDS             complement(152..514)
                     /locus_tag="IW746_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079271.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            712..1953
                     /gene="sstT"
                     /locus_tag="IW746_002390"
     CDS             712..1953
                     /gene="sstT"
                     /locus_tag="IW746_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1079272.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2100..2735
                     /locus_tag="IW746_002391"
     CDS             2100..2735
                     /locus_tag="IW746_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1079273.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            2926..3666
                     /gene="truA"
                     /locus_tag="IW746_002392"
     CDS             2926..3666
                     /gene="truA"
                     /locus_tag="IW746_002392"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1079274.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3811..>4743)
                     /locus_tag="IW746_002393"
     CDS             complement(3811..>4743)
                     /locus_tag="IW746_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079275.1"
                     /translation="IPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGL
                     FLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLN
                     GFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIA
                     PEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNS
                     NNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAI
                     ASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         1528 a          700 c          934 g         1581 t
ORIGIN      
        1 aattgttaaa aataatagta gatatttaat tgttgttttc gataatttat ttaaaaatcc
       61 aactgtataa ggaatactac aaaaaagcaa agtacaagaa acagcataag taaagtaatt
      121 ctgactgata ggtgttagga taagccaata atcacgaata ttcaagctgc cagcttgtac
      181 gtaaaaaaaa gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta
      241 aataatgtaa aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga
      301 tcaaatttgc accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag
      361 ggtttgttgc atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca
      421 ttatacaagc gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga
      481 ataaacacct cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc
      541 cattaataac atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca
      601 aaattagcaa gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt
      661 tctttgcata aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag
      721 aagtttttgg atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg
      781 tttggtatct ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt
      841 tgcttgaagg caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag
      901 cataaaattg ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca
      961 ttgctttctg cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta
     1021 caagaaacag tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg
     1081 acgaatgtca cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta
     1141 ttctgggctg tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg
     1201 gtggatcaga tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg
     1261 atagggattt taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca
     1321 gagtatctgc agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg
     1381 tacccattcc tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt
     1441 ttgaaagaaa gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata
     1501 aacatgatgt tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg
     1561 ttaggtgcta cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact
     1621 gctgtacatt ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg
     1681 tctgctttag ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca
     1741 ttagcatgta gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga
     1801 tttattatcg gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg
     1861 ttgtttactg caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg
     1921 aaagattgtt tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat
     1981 gattcgagtt tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca
     2041 agataaagtc gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca
     2101 tggatttttt acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt
     2161 tgactccgta cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca
     2221 gtgtatttgg agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt
     2281 cagggatctt tttcatcctg tcattgcctg cattgtatgc tgcatttgta agtacatctc
     2341 gtgtgttagc taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct
     2401 ttacaggttt gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc
     2461 ataatatcgg atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct
     2521 tgatctatcg aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga
     2581 tcagtttgct ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc
     2641 cattactgaa taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca
     2701 ttgcctggtt gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt
     2761 cagcggatac ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt
     2821 ttcttgtata gcttctgttt tagagaagaa aggagctata ttttgtgcta caatcttaaa
     2881 aaggtagaga gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag
     2941 ttgacaatcg aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat
     3001 aaaaccattc aaggaaaaat agaaaaagta ttgcatcaga tgacaaatga agagatagaa
     3061 attattggat caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt
     3121 aaaacaaaca cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg
     3181 cgggatattg tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat
     3241 gcggtgggca aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag
     3301 cgaaaccaca gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc
     3361 gaaaaattag tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa
     3421 tccacagtgc gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt
     3481 acttttgtcg gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta
     3541 gaaatcggca caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg
     3601 cggcaacatg caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat
     3661 cgataaaaat actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta
     3721 aataatgtat gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt
     3781 acagctaaaa agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg
     3841 ttgtttttgt ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga
     3901 taaatatctt gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat
     3961 acctggagga atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt
     4021 gccattaata gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt
     4081 tttattattc gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg
     4141 atttatttcc tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat
     4201 tgactctttg tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc
     4261 aggtgctata agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag
     4321 atgcaatatt agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga
     4381 aaaaatataa tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt
     4441 taaccgccag acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa
     4501 taaattggtt gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc
     4561 agttaatttt tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat
     4621 agtatagttg ttatagttat tatttaaaat gtgtgtcagc atatcactag gagccatacc
     4681 acttaattct tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg
     4741 aat
//
LOCUS       DACEND010000150         4740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEND010000150 DACEND010000000
VERSION     DACEND010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.156"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..770)
                     /gene="xth"
                     /locus_tag="IW746_002394"
     CDS             complement(18..770)
                     /gene="xth"
                     /locus_tag="IW746_002394"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1079276.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            883..1803
                     /locus_tag="IW746_002395"
     CDS             883..1803
                     /locus_tag="IW746_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1079277.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1876..2811
                     /gene="murB"
                     /locus_tag="IW746_002396"
     CDS             1876..2811
                     /gene="murB"
                     /locus_tag="IW746_002396"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1079278.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3109..4587
                     /locus_tag="IW746_002397"
     CDS             3109..4587
                     /locus_tag="IW746_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1079279.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1677 a          751 c          912 g         1400 t
ORIGIN      
        1 actttttcac aacctcttta tttcaaatct aattctactg gacaatgatc actgcccata
       61 atgtccgtat ggatctttgc atcagctagt cctggctcta aatctttgct cgtcaaaaag
      121 taatcgatcc gccaaccagc attgtttttc cttgaattga atcggtagtt ccaccaagag
      181 tagacacctt cctgggttgg ataaaaataa cggaatgtat cgataaaccc gttatccaac
      241 agtcgagaaa gagccgctct ttcttctgga gtgaatccag catttttttg attcgttttc
      301 cagtttttca aatcaatctt ttgatgagcg acatttagat ccccacacac gatgactggt
      361 ttttgttttt ttagattttc caaataatta taaaaggctt cttcccattc taatcggtaa
      421 tctaaacgtt ttaattcgct ttgagaattt ggcgtgtaac aggttacaag aaagaaatcg
      481 gaatattcca aagtaatcag acggccttct gtatcatgga tatcgatacc cattccgtaa
      541 gtggcattca atgctggttt tttagcaaaa atcgctgtgc cggaatagcc ttttcgttcc
      601 gcgtaattcc aatattgata ataacctgga agatctaaat caatttgtcc tgcttggagt
      661 ttcgtttcct gcagacagaa aaaatcggcg tctaactcat gaaaaacttc taaaaaattt
      721 ttatttacga tggcacgaag cccgtttacg ttccaagaga taaatttcaa taaaagcaca
      781 ctccttcgaa ttaatttcct tacctttgcg atactattgt acctcatttt agtaaaaaat
      841 ggtgtaagat gaaagtaact aaatgatcca ggaggaaagt agatgaagat tggtgtaatc
      901 ggtgtaggga atatcgcaga aaaagcatat ttaccaactt atgcgaaaaa acaaggaaca
      961 cttgattttt attttgcaac aagaaataaa gaaacaaaag aaagaattca aaaagagtac
     1021 gggttccaac atctatatga aacgatcgat gagcttttag aagaaaaaat cgaagcatgt
     1081 atgatccacg cagcaacaaa agtacattat gaattagcca aaaaatgtct agaaaatggt
     1141 atccatgttt atattgacaa accactgagt gtccatctaa atgaagttcg tgaattgcaa
     1201 gaattagctg ataagaacca aataattttg atgatcggtt tcaaccggcg ctttgctcct
     1261 atggtagaag aattgaaacg tatcccagat aagcggttga tccacctgca aaaaaatcga
     1321 atagcggcaa aagaaacgac agagttcgtc ctttatgatt tgtttttaca tcttattgat
     1381 acagctgtgt atttattaga tgaacccatt ttgagaacaa aataccagat aagagaaaca
     1441 aaggaatttt tggaatatgc cattttacaa ttagaaacac aaaatcaaac ggctatttta
     1501 acgatggatt tagcaagtgg cgctaatata gaaaaatatc aagtaacaag taaaaatggt
     1561 acttatgaag taaacgatct gacccagctt gtcattcaag atgctgatgg aaaaaaagtg
     1621 aaagaatttg gcgactggac aaacacgcta gtcaaacgcg ggtttgagcc aatggtagag
     1681 gccttctttt ctcagatccg tttaggtaaa tcggaaacag aattgcttaa acaaaaagaa
     1741 gttgtaagaa gtcatgaatt atgtgaggaa gtgcttaaag atcactaccg tcatcaactt
     1801 tgaacaagac tggcaagatt gtattaagcg tgatagaata atgaggacgt ttgagaaaag
     1861 aaaggaaaat cctcagtgaa taaagaagaa atagttgaaa gcttagccac gattactata
     1921 ttgtttgatg aaccattgat gaactatacg tttacgaaaa caggtggacc agctgatgtc
     1981 cttgcattcc cgaaaaaaca agaagaagta aagcaaatca tcgattattg ccgaatccat
     2041 gatatacctt ggatggtatt aggaaatgca agcaatctga tcgttcaaga tggcgggatt
     2101 cgcggtgtag tcatcatgct gactgaaatg aaacagattc atgtcaaagg aacgatggtc
     2161 attgcggaag caggtgcctc actgattgat acgacttatg cagctttggc agaatcattg
     2221 actggatttg aatttgcttg cggcatcccg ggaagtgtcg gtggagcggt ttatatgaat
     2281 gcaggtgcgt atggcggaga gatcaaagat gtttttgctg aagtggatct tttgcttgaa
     2341 gatggaacat tgaaaacttt gacaaaagac gaaatggtat tttcttatcg tcacagtaaa
     2401 gtccaagaat tacgtgctat cgtattggaa gctcgattca gtttgcaaac aggggattat
     2461 gaagcaatca aagcaagaat ggatgagttg accgaattgc gtcaatcaaa acagccatta
     2521 gaataccctt catgcggaag tgtctttaag cgcccagtag gacactatac aggtcagctg
     2581 atccaacaag caggattgca aggcctgaaa tggggcggtg cacaagtctc tgaaaaacat
     2641 gctggattca tcgtcaatat cgatcatgcg acagcgacag attatattga attgattgcg
     2701 cacatccaag aagtaatcaa agaaaaattt gatgtgtctt tagaaacaga agtgcggatc
     2761 attggagaaa aacaagcaat atcagaaaca agcagaagtt tagccaatta ataagtaatg
     2821 gaacttaaaa taagtaaaag agcaggacgg tggccgtcct gctctttttt gtggttgttt
     2881 acgcaaatgg cgcaaatctt gtacatttta acacaataaa acaaaaaata tagaatattt
     2941 cctcataaat ttttccattt tttgcaatat tctcaaaaaa aagataaaaa atgagaataa
     3001 ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag atgaactttc
     3061 atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat gaagttggat
     3121 taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt agaatttgag
     3181 aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc taaatttaaa
     3241 acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac aaagccagag
     3301 atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac ggatatcaaa
     3361 aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac ggagatcgtg
     3421 gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat atcaagagcc
     3481 tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa gttaaagaaa
     3541 aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt atatttcggg
     3601 gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc aaagagtatc
     3661 gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg aatcaaattc
     3721 aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat cggaaaacgt
     3781 ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa ggatttatca
     3841 catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc ttatatcatg
     3901 ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact tgcggaagct
     3961 cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat tgatcaatta
     4021 ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga acttcgtatg
     4081 ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac tagctttacg
     4141 tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac agcaatccaa
     4201 aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt catgattcgc
     4261 ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata ttttgaagca
     4321 ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta cattatttct
     4381 caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg
     4441 aatatttatt tatctgattg tttacttgaa aaatttaata aagaacagat aatttggaaa
     4501 aatccaccga ctccagatga ctgggaatat tttggaaata ccatcatcag agaaaaaaat
     4561 agacatttaa ctgaaaaaat cagctagtat ttatggatgg cagctttgtc tcacatattt
     4621 attagctgat ttatgttttt ttcaaagaaa aggtaactag atataactag atataactag
     4681 atatattact taacaaaatt aaaaagtaca taagttatta ttccgatttt cttgaaacaa
//
LOCUS       DACEND010000151         4693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEND010000151 DACEND010000000
VERSION     DACEND010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.157"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1055)
                     /locus_tag="IW746_002398"
     CDS             complement(93..1055)
                     /locus_tag="IW746_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1079280.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="IW746_002399"
     CDS             complement(1057..2496)
                     /locus_tag="IW746_002399"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1079281.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="IW746_002400"
     CDS             2681..4627
                     /locus_tag="IW746_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1079282.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1493 a          801 c          770 g         1629 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tat
//
LOCUS       DACEND010000152         4653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEND010000152 DACEND010000000
VERSION     DACEND010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.158"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..482)
                     /gene="dfrF"
                     /locus_tag="IW746_002401"
     CDS             complement(9..482)
                     /gene="dfrF"
                     /locus_tag="IW746_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1079283.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(901..1119)
                     /locus_tag="IW746_002402"
                     /pseudo
     CDS             complement(901..1119)
                     /locus_tag="IW746_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1150..2364)
                     /locus_tag="IW746_002403"
     CDS             complement(1150..2364)
                     /locus_tag="IW746_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1079284.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(2705..2947)
                     /locus_tag="IW746_002404"
     CDS             complement(2705..2947)
                     /locus_tag="IW746_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1079285.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2979..3536)
                     /locus_tag="IW746_002405"
     CDS             complement(2979..3536)
                     /locus_tag="IW746_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1079286.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(3549..3737)
                     /locus_tag="IW746_002406"
     CDS             complement(3549..3737)
                     /locus_tag="IW746_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1079287.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3750..4316)
                     /gene="amaP"
                     /locus_tag="IW746_002407"
     CDS             complement(3750..4316)
                     /gene="amaP"
                     /locus_tag="IW746_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1079288.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1388 a          982 c          713 g         1570 t
ORIGIN      
        1 tactcaattc attttttcct tacataaaat gttctcgtat atttcacttc ctcaccaagt
       61 gtttccccta tcaaaacttc aaaatcattg atatcaaatt ctggaaagaa tgtatctcca
      121 tcttcaatat ttaaatctac ttctgtgata tacattttat ctactatttg caaagcttcc
      181 ttaaatagtc catatccacc agatatgtat acatctcgtc ctttagccaa taataatgca
      241 tcttctaatg atttaactga aactaaatta tctccttgat actctgttgt ggtggaaaca
      301 acaatattca ttctattagg caacggatga ccgatttctt cataagactt tcgccccata
      361 ataaccacat tacccgttgt taactctcta aattgctttt gttctccctt tattttccat
      421 ggtatattac cattcttgcc tataacatta ttctttgacc tcgcaacaat caaacctatc
      481 atctatttca tcctccataa tatgatgtcg tacctgtgat tccatcaagt gcaccacatc
      541 ttaacaaact caaaattatc tcgaactaac cagctcgaca aattcctttt ttgtcttcat
      601 ctgcattata tcatattgtt ataagcattg ccatttcttt tctgcttttg aaaagttact
      661 tatcagccta tgttttgtaa agggtgatat tcagcatttc tcacgcaaca agcccttgac
      721 aaaccataga ctaataagtt ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca
      781 cggcaacaaa aaagagatac caacacactt atgtagtatc tcttagtcta gtagcaatat
      841 tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa tatctttttg tttgggaaac
      901 acctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
      961 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     1021 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
     1081 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
     1141 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
     1201 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
     1261 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
     1321 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
     1381 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
     1441 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
     1501 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
     1561 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
     1621 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
     1681 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
     1741 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
     1801 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
     1861 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
     1921 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
     1981 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
     2041 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
     2101 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
     2161 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
     2221 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
     2281 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
     2341 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
     2401 gaaggtgtag ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac
     2461 agacaattaa taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac
     2521 aaaaaataaa cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttattttta
     2581 aagatatttt ttcataagga ataaagaata cttcatataa aatatataaa aaagaaccgc
     2641 agcgagtgtc gctacggttc tcaaatttgt tcttcatttc aacataagtt tttcttcaat
     2701 ttgcttataa tttacgtgca aagaatgaaa cgactaatac taaaatgatt gcaccgatca
     2761 atgatggaat gatagccatt cctgcaattg ttggtcccca agttcctagt aaagcttgtc
     2821 caattgctga acctactaaa ccagcaatga tgtttgcgat gatccccatt gatgatcctt
     2881 tattcgtgat tgctcctgca attgctccaa tgataccgcc gacgattaat gcccaaataa
     2941 atcccatttt aaattcctcc taactgactt cttacttctt attgtacacg tggttcgtta
     3001 ttttctttga ctttttgtgc acctttgcct acagcagatt ttgctttatc tgtttggcgt
     3061 gaagcaaatt gtcctgtgga ttttgctgct tctgttactt tgtcttgtac tgtttcttga
     3121 tctttttggt attcttcttg tgttttgatg tctgcaacat tcacattcac ttcgataaca
     3181 tctaaatgtg tcatattgtt tacttcagta ctgatcaatt ctttgatttg gttgtaaata
     3241 tcttcgatgt ctttaccgta ttctacgatc acatctagat ccacagctac ttgtttctta
     3301 cctacttctg tattgatacc tgctgttacg ttatctgtgt tgactaattt ttcagccata
     3361 tttgagaaaa atccgccatc taatgtcaat agaccatcta catctttcaa tgcataaccg
     3421 atgatttttt ggatgacttt gtcttcataa gtcaattcac cattcatcat gcggttttct
     3481 cctttttcat ttgccaaaac tgatccattt gttgtattca tgtttttgtt ttccatcttt
     3541 gtttcctcct atgatttaaa aaattgatca actattcctg actgtttcaa ataaaaaccg
     3601 attcctgcgc caattgctgt catgattatg atcaacaatg tcttgaaaaa tccaatcgtt
     3661 aaccataaaa cagctagcag tagtccaaga cccccaaaga taatagggag cttgtatgct
     3721 gaaaatagtt ctttcattat ttggccctcc tattccactc gagagtgctt gtagtctctt
     3781 gtatcttctt gttcttcata accgctgtat gtgactgcca ctttgaccgt ttcttttgtt
     3841 ccgagtactt tagttacttc ttcttcgata tcttgcatca atacacctgt tttccctact
     3901 aatgaagaag ttcgtttcaa gtctccgttg acatggatat ggattcgatt tttcgttgaa
     3961 cgtatccgta ctttaggtga atgaatgaac tcttcttcgt gtaaatggga acgtaccaac
     4021 ccttcgatcg cttttttgtc gattgttagt ttgccatctt cccgtttcat aacgaaagtt
     4081 cctcttgact ttggatagaa aagaacgact agcaagatga taatagcaag aacggcaaat
     4141 actacagctg cccagaaaat atattgttgc atgtagtaat tcgttaaagt aaagaagcga
     4201 aatcgttcca attgttcagg cataatatac ggcgcattga tcaacgcaat cgctagaaat
     4261 actgaaagta gcagtaaact catgataaca ccgattattt ttaacccctt attcattttc
     4321 cacctcctat aaacttattt ttaccctgta aataactcta ccgacaatca cagtatagat
     4381 agcgaacagt tagcatagca tcagtaaata aagcgctacc aaacattgct ggatcataac
     4441 ggcgttttat tgccagtctg atactgaaat aacaattgct tacttattct ctatttactt
     4501 ttgattgctg gctatactta aaggatatta ttcaagatta aaaattgcaa agattatgct
     4561 cagttattat tacaagattc tatttattta ttaaattttt tcggagaatt aaaaaataca
     4621 ataaataact ttagttactt taatgcgttc tta
//
LOCUS       DACEND010000153         4435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEND010000153 DACEND010000000
VERSION     DACEND010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.159"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..564)
                     /locus_tag="IW746_002408"
     CDS             complement(109..564)
                     /locus_tag="IW746_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079289.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IW746_002409"
     CDS             complement(718..1926)
                     /locus_tag="IW746_002409"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1079290.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LYFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IW746_002410"
     CDS             complement(2038..2370)
                     /locus_tag="IW746_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1079291.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IW746_002411"
     CDS             2518..3393
                     /locus_tag="IW746_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1079292.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IW746_002412"
     CDS             complement(3475..4056)
                     /locus_tag="IW746_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1079293.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..>4435)
                     /locus_tag="IW746_002413"
     CDS             complement(4062..>4435)
                     /locus_tag="IW746_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1079294.1"
                     /translation="LKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQMEETANPY
                     AEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRAQELLTY
                     YLTLEPGDMEALSLLDDLTER"
BASE COUNT         1230 a          925 c          753 g         1527 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatacaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcactt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagta
//
LOCUS       DACEND010000154         4305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEND010000154 DACEND010000000
VERSION     DACEND010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.160"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..1137
                     /locus_tag="IW746_002414"
     CDS             148..1137
                     /locus_tag="IW746_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1079295.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IW746_002415"
     CDS             1134..1553
                     /locus_tag="IW746_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079296.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IW746_002416"
     CDS             1562..1870
                     /locus_tag="IW746_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079297.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IW746_002417"
     CDS             1874..2236
                     /locus_tag="IW746_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079298.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IW746_002418"
     CDS             2458..3864
                     /locus_tag="IW746_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1079299.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACEND010000155         4247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEND010000155 DACEND010000000
VERSION     DACEND010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.161"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..602)
                     /locus_tag="IW746_002419"
     CDS             complement(72..602)
                     /locus_tag="IW746_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1079300.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            707..1258
                     /locus_tag="IW746_002420"
     CDS             707..1258
                     /locus_tag="IW746_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1079301.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1411..1884)
                     /locus_tag="IW746_002421"
     CDS             complement(1411..1884)
                     /locus_tag="IW746_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1079302.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2022..3494
                     /locus_tag="IW746_002422"
     CDS             2022..3494
                     /locus_tag="IW746_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1079303.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3491..4201
                     /locus_tag="IW746_002423"
     CDS             3491..4201
                     /locus_tag="IW746_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1079304.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1328 a          763 c          826 g         1330 t
ORIGIN      
        1 gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt cacaacctct
       61 ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc gctacggata
      121 tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc tatacctgca
      181 tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata agtttcatat
      241 ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac agtgatttct
      301 ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt ttcatttggc
      361 ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt gaatacgccg
      421 tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt gaccattccg
      481 ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata ttcccaacca
      541 ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt aaattctttc
      601 atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt tcgattactc
      661 atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg caagaaaaaa
      721 tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag gatatgaatt
      781 gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata gtcagattta
      841 tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag agatcacagg
      901 tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac aattggcaga
      961 gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg ttctgaaatt
     1021 gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc agcagcgtga
     1081 ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct ttcctactga
     1141 aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga tcaatcggga
     1201 aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa aaaaataaaa
     1261 aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag atcaccgttc
     1321 ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc tacttattaa
     1381 aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact gttcgtagtc
     1441 agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt tgtcatgaat
     1501 gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg tatagcgttc
     1561 gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat ggtgcatttt
     1621 cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt catctaaata
     1681 ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag gacaattcgc
     1741 atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt gacgtggaga
     1801 cacacctacg atatcactta attcactgat gccaacaacc aattgatcat tttccaacaa
     1861 acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc ttcccttaat
     1921 tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta ggactaaagt
     1981 attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac aaccaacccg
     2041 tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta ttaattggta
     2101 cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca ttgatgaaag
     2161 ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg ctagtcaacg
     2221 ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga aatttatatc
     2281 tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca cctaatttta
     2341 gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg atgccattat
     2401 tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca atggggatgt
     2461 cagggatcgt tattggactt gctcctgctt taggccctac attatctggt tatatcattg
     2521 ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt cttgtcttgg
     2581 ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc attgatattt
     2641 tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt tctagtgtag
     2701 gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt gtgattttca
     2761 tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa cttcgtgtat
     2821 tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat atggcgatgg
     2881 taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa tcagcattcc
     2941 attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg cctgtgacgg
     3001 gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg ttcattttga
     3061 cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta tatatcgtga
     3121 tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta acgacttctg
     3181 gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat aatacattcc
     3241 gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg aatgtgacaa
     3301 aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat aaagatcagg
     3361 caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc atctttggga
     3421 ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta atgaaagcgg
     3481 gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg ctttaacgaa
     3541 cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac taatcttcgt
     3601 cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg aaaaagtaac
     3661 ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag atatgctttt
     3721 gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa atgaaaaaca
     3781 agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa agacagacgg
     3841 cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca tgtataaatt
     3901 ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata cattcaaaat
     3961 cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg aattagtgaa
     4021 gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg gtatggtaaa
     4081 agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta cgaaagcctt
     4141 agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa aaggcgaata
     4201 atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatc
//
LOCUS       DACEND010000156         4158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEND010000156 DACEND010000000
VERSION     DACEND010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.162"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            84..1385
                     /gene="murA"
                     /locus_tag="IW746_002424"
     CDS             84..1385
                     /gene="murA"
                     /locus_tag="IW746_002424"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1079305.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1398..1574
                     /locus_tag="IW746_002425"
     CDS             1398..1574
                     /locus_tag="IW746_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1079306.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1709..2005
                     /gene="yidD"
                     /locus_tag="IW746_002426"
     CDS             1709..2005
                     /gene="yidD"
                     /locus_tag="IW746_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1079307.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2190..2657
                     /locus_tag="IW746_002427"
     CDS             2190..2657
                     /locus_tag="IW746_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079308.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2742..3626
                     /locus_tag="IW746_002428"
     CDS             2742..3626
                     /locus_tag="IW746_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1079309.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3743..3976
                     /locus_tag="IW746_002429"
     CDS             3743..3976
                     /locus_tag="IW746_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1079310.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4064..4144
                     /locus_tag="IW746_002430"
     tRNA            4064..4144
                     /locus_tag="IW746_002430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4098..4100,aa:Tyr,seq:gta)
BASE COUNT         1437 a          640 c          934 g         1147 t
ORIGIN      
        1 gtactatttt aaaagcaagg atgttataat agaaaagatt ttgagtaagt gtatgctcat
       61 tggaattttt tggagggaac aacatggaag agatcatcgt aagaggtggc aatcaattaa
      121 atggaacagt aagaattgaa ggagctaaaa atgctgtttt gcctatttta gctgctagtt
      181 tgttagcaga agaaggtatt acgacattag acaacgttcc gattctttct gatgtcttca
      241 ctatgaatca agtgattcgt cacttaaacg tagatgtaga ttttgatgaa caaaagaatc
      301 aagtaacaat cgacgcttca agacaattag aaatcgaagc gccttatgag tatgttagtc
      361 aaatgcgtgc ttcaatcgtt gtgatgggtc cgctacttgc cagaaatgga catgcaaaag
      421 tagctatgcc tggtggatgt gcgattggta aacgaccaat cgatttgcat ttaaaaggat
      481 ttcaagcttt aggagcaaaa attattcaaa aaaacggcta tatcgaagca attgcggatg
      541 aattgatcgg taacacgatt tatttagatt tccctagtgt aggtgctact caaaatatca
      601 tgatggctgc cgtaaaagca aaaggaacaa ccatcattga aaacgtagct cgcgaaccag
      661 aaatcgttga tttagcaaac attttgaata aaatgggcgc tcaagtctat ggagctggta
      721 ctgaaacaat gcgtattgaa ggcgtggatc atttacatgc agtcaaccac tcaatcgtac
      781 aagatcgtat cgaagcaggt acctttatgg ttgctgctgc aatgacacaa ggcaatgtgc
      841 tgattgcaga tgctatctct gaacataacc gcccattgat ttctaaattg atcgaaatgg
      901 gtgcagaaat cattgaagaa gaaggcggag tccgtgtcat cggaccaaaa catatcttac
      961 ctacagatgt aaaaacgatg cctcatcctg gtttccctac agatatgcaa gctcaaatga
     1021 cagctatcca attagttgca gaaggtacaa gtgtagtgac tgaaacagtg tttgaaaatc
     1081 gtttccaaca tttagaagag atgcgccgta tgaacgctca tgtcaaaatc gacggaaatg
     1141 tagcaatcat ggatggaaac catgaattgc aaggtgcaga agtatatgca acagacttgc
     1201 gggcagcagc agcattagtt ttagctggct tgaaagcaaa tggaatcaca cgtgtgcgta
     1261 acttgaatta tctagaccgt ggatattata atttccatat caaactacag caattaggtg
     1321 cagacgttga acgtgtagac atggatcaaa cttctgctga aaaaacagca caaacgattg
     1381 cttaaggaag tggctgaatg agtaaccaac gatatatctt attaactttg ataaagattt
     1441 taatggtgat cttattgctt atccttcttt ttgtggccgg aacgatgatc ggatatggtg
     1501 tcatcggtgg cggcaatcca tttaaagtat ttcagccaag tttgtggatt catattcgag
     1561 acttttttca ttgataaata agaatcacag tggttctagt cgatatgaag tgagccatga
     1621 agcagaacat gataaaagaa aacagaaggg aacgttgacg ttagtcgcga tccctttttt
     1681 tagtaaaaga aaagaaggaa tctaattgat gaagaagata ttgatttatt tagttagagg
     1741 atatcagcgc tttatttcgc ctgcatttcc acctagctgc cgttattacc caacctgttc
     1801 caattatatg ataaaagcaa ttcaaaccca tggcgcgcta aaagggacgg taatggggac
     1861 tgcacggatc ttacgctgtc atccttttgt caaaggcggt atcgattatg taccattgac
     1921 attccgatta acgcgaaata agaaagataa aaaagaacca gattataaaa aggttgtgaa
     1981 aaatacgggt agcactgagc gataaagaaa caatttggaa aatagcttct tatattttgc
     2041 aaaattgtga gttattgccg aagtgttgga ttttgaacac cgtcaaaaaa aaggttgtga
     2101 aaaatacggc tagcactgag cgataaagaa aaagaggaaa tttattgtca aagtaacaac
     2161 tccgcaacta cttatatggg ggacaaaaaa tgatcgaaga actaaacaag aaagatttac
     2221 catggtcctt gttattagat gcagatcctg ataaagaaaa agttcaagta tatgtttcca
     2281 gagggagtgg ctggatatgg aaagaaaaag aaaaaacaat aggtgtactg atctatgtgg
     2341 caagggaaaa agaatacgaa atcgtcaatg tagcagtaac cccaacgtat caaggaaagg
     2401 ggattggcgg aaaattactt gagacagctt ttcagaagct ttcacaactc gcttccagtc
     2461 aaactcagat catcatacga accggcagta ctagcagtgc ggcgttacat ctctatcaaa
     2521 aaatgggcta tgtggagatt ggtcgagtca aagactattt tatccatcat tactctgagc
     2581 ccatttttga aaatggagat caattaaggg atcaagtgat tttagcaatt cacttaccta
     2641 aaaatagctt taactaataa aatttcaatt caagaaaatg ctttcaaatg ctaaatagat
     2701 acattacaat agctttagca aacaaaaagg agaatatttt tatgggaata ttagtttttg
     2761 attttggtgg atcggctgtc aaatatggtt gttgggatgg caaagagatt aaaggtaagg
     2821 gacaatttgc aacgcctgaa tcttgggaag aaatgaaagc gcaattattt caagtttata
     2881 aaaaaatact cgtttctttt gaagtcgaag gaattggaat cagctctcct ggagtagtga
     2941 ataatgagaa acaggtgatt gagggaatca gtgcgattcc ttatattcat ggatttaata
     3001 ttttccgaga tttggaaact ctgtttcaac ttccagtaac gattgaaaat gacgccaact
     3061 gtgccggaat ggctgaattt tatgaaggtg ctgcccgcga ttatcaagat gttgcttttg
     3121 ttgtggcagg aactgggatt ggcggggcgt tgttcacgaa aggacaaatc aataaagggg
     3181 cacatcttta cggtggagaa tttgggttga tgttcttaaa tggcgataag acattcagta
     3241 aacttggtac agcggtacag atggcttggc gttattgtga acgcaaaggt ctcgataaaa
     3301 atgcttatac cggaaaagat gtctttgaat tagcagaaac tggagatgca atagcaaaag
     3361 aagaagttga gagtttttat acgtatctta caaagggatt attcagtatt caattttcgt
     3421 ttgatccaga agtgatcgta ttaggcggcg gggtttctgc aaaagaaggg ctcattgatg
     3481 agatcaaaag ccgaatgaag aagctgacag aacaattcga tctgcatgat ttcgaaccgc
     3541 agattttatt atgtgaatac agaaacgatg ctaatcttgt aggtgcagca gcaaactatc
     3601 tcgctgtacg tcaaggggag aattaagagc tttagagaag gtgcagcttg cataaaagaa
     3661 gtgaatgaaa tgaataggag atttttacct aaggaatgtt ttatttgctg cagtatgata
     3721 aaatatctga gaggtgaaaa tgatgtcaaa aaaaaataaa gatattgaag tgcgtataga
     3781 agaagtgaaa aaagatatca aaggtaagaa ttatgaagtc actcagttat ttatagggaa
     3841 aaaaatgatt ggtgagatct tagcctatgg accaaaagaa tatgaaattt tctttggtga
     3901 agaagatttt ggaaaagaaa aaagtcttga aaatgcgata gaaacagtga ttcgacactg
     3961 gaatctgcac gaataaaaaa atatcaaaaa aactttcaat aaagggttgc aaaaatctgg
     4021 aaaattaggt attataacta agtcaggttg atacaaacct ttcggaggag tagcgaagtg
     4081 gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa tccactctcc
     4141 tccatttcat tggggtat
//
LOCUS       DACEND010000157         4156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEND010000157 DACEND010000000
VERSION     DACEND010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.163"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..884
                     /locus_tag="IW746_002431"
     CDS             36..884
                     /locus_tag="IW746_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1079311.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1029..1970
                     /locus_tag="IW746_002432"
     CDS             1029..1970
                     /locus_tag="IW746_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1079312.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            1960..4131
                     /locus_tag="IW746_002433"
                     /pseudo
     CDS             1960..4131
                     /locus_tag="IW746_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1278 a          829 c          773 g         1276 t
ORIGIN      
        1 aagcggtcat cggcaaacgt tcaactatcc aattgatgat cgtggcccta ttagcaggtg
       61 gtcatgtctt attcgaagat attcccggtg taggtaaaac gttactggtc aaagcactag
      121 caaaagctgt acagggaacc ttcagcagag tccaatgtac tcctgatctg ctgcctagtg
      181 atattcttgg tttttccgta tacaattcac aatcaaaaga gtttgaattt cgtccgggac
      241 cgatctttac cacgatcttg ttagcagatg agatcaatcg tacgactcct cgtactcagt
      301 cagcactttt agaagcaatg gccgagaatc atgcaacgat cgacaacaat acataccctc
      361 tagacaatca tttttttgtc ttagctacgc aaaatccaat cgagtacgaa ggaacatatc
      421 ctttgccgga agcacagctc gatcgttttt tattccgact acaaatcggt tatccttctt
      481 ttgatgacga acttttgcta cttttggata aattcgaaaa agtgttggat aacttaaatg
      541 aagtattgaa cagttatgaa atagaagaaa taaaaacaaa tgtggcgaat gtctttgtca
      601 gtcccgaagt ggcatcttat gccttgcagt tagtgtcagc aacaagaagt catgctgcca
      661 tccagcttgg catcagtcca cgaggaagcc tttcctttat tcaggcagca aaagcttatg
      721 ctttaatcca tgggagaaat tacgtcacgc ccaaagattt gcaggattta gttccttacg
      781 tgttttccca tcgcctgatt ttttacgatc gttcattgaa ggaagacgaa aagaaacaaa
      841 tcattcatac tcttgttgca caaattccta ttcctgtgag gtagattgag gtagacaatg
      901 gaaaaaatca aagattattt cattaatttt gtgttatttc tggtatattt catcattgtg
      961 ctttttgctc ttgtattcaa caattcttta ggctggttct tgttggcctt ttttactctt
     1021 tatgtatcat ggagcttgat ttcattaagc agctttctaa agaaactttc ggttacttgt
     1081 acattgcagc cactcgttta tcgcaaaaaa agtatgcagg ttattttttc catagcaaaa
     1141 aagagaactt tttctttacc tctccctcgt ttacaaatcg gcttcccagc aatctttgca
     1201 gaaaaaaaca agagattttg attcttacaa ataaaccgca agaagtccaa ttgctttggc
     1261 agccaaaaga aagaggatgc catgaaacat taactgtcat ttttacagcc tatgatagtc
     1321 taggactttt cagaaaaagt gcaaaaagag agatctcttt tcctgtgact gttctgcctg
     1381 ctttttgtaa gattcaagca gaaaaattac agagagttct ggaaaaaaaa cagcagctga
     1441 acgcgtacca aagaggtctt gattttcggg aacatcgcgc ttatcgtcca ggtgattctt
     1501 tcaaccgaat cgattggaaa ttgtctgctc atactcaaga gtggttatac cgtgaatacg
     1561 attatcaaga agaagaatgt tctcctcttt tttgcttttg gggttcgccc agtccaaaat
     1621 ttgaacctct gctagacttg tatttttctt tatggcattt gagaaaagaa aagcagccgg
     1681 aacttctgat attaggcaac cgcgcctttc atggcaaaga ccccggtcat acctattttg
     1741 cttctttgga agctggcgat gagaaaactt ttttcgcaca cttgaaaaaa tatgcaaaaa
     1801 aacaaatcgt cctctttgtt cctgagaatt ctcctgaagt ctcagaagct attgaaacat
     1861 tgtcaaaaga tcgaacggtt tatcttgttt atttcgtaga aaaacaactc atggtccaag
     1921 aaaaagacaa gatatgttca ttgccaggag gtgaatggat tgacgattaa acgggaaaaa
     1981 tggctgcgtg gcgtttactc ctttgtgata ctaagtgtag gtgctccgat atttgtagta
     2041 gctaatcaat tgcaaaacag ctaccttctt atcctaacta ttggtgtcct atgccttatt
     2101 gtctgctgta ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt aatgataggt
     2161 tgtttgtttt tatattttcc ttcgaagaca ttttccatat tatggatctt acagtttttt
     2221 tcgtcagtta cagaggaatt cttccatcta tcccaaggta aactttatta tttgccagaa
     2281 aagacagctt tttttctcat aatgttgctg atctatttat ttattacatt gaccgtggat
     2341 tacgactatt ggtacgcacc ctttctttct ttgatggctt acttcatgat gcttaccgtt
     2401 tttcggaaaa aagatttact gtacgaaatg attgcggtag taactgtttt gttcgtgtat
     2461 ctggcagcaa ggcaattttt ctccatccgg ttgttgtcga gaaacactcg attccagtca
     2521 ttgactacag ttctgcttat ttgtttctta acttttagcg ctatcttgcc tttatatctt
     2581 ccgaaagtac accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat
     2641 gaagggcttt atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc
     2701 agtgagaatg atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca
     2761 gcgaaccaaa aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct
     2821 ggatgaatag aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc
     2881 acgagctacc aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa
     2941 gctttcttac cttatcctta tggaaatact accttgactg atttttcatt agctagtgga
     3001 caaacgcttt actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca
     3061 cgttctgcaa ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt
     3121 cagttaaaag ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg
     3181 cctaaacgta ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt
     3241 aaagtcgaag ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg
     3301 gataccgggc atactccaaa agaacacgat tatgtggatt atttcctctt tgattcaaaa
     3361 gtcggctatt gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc
     3421 ccagctcgct ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg
     3481 aatgaggaat acagcattct caatagtcat gctcattctt ggccagaggt atactttcct
     3541 ggatttggct ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa
     3601 ccagttgctg aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa
     3661 aatatggcag aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca
     3721 ctcacaccag cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg
     3781 ttctttctat tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat
     3841 ttttttgctt ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg
     3901 gtaccgcgca aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc
     3961 aagctagatc tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga
     4021 caagcagatc cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga
     4081 aaaacggtat acaaaaaaat tctgacaaaa gggagccgct tcaaaaaata agataaaatt
     4141 tctggagaat ctactt
//
LOCUS       DACEND010000158         4074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEND010000158 DACEND010000000
VERSION     DACEND010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.164"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1051)
                     /locus_tag="IW746_002434"
     CDS             complement(77..1051)
                     /locus_tag="IW746_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1079313.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="IW746_002435"
     CDS             complement(1500..2915)
                     /locus_tag="IW746_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1079314.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="IW746_002436"
     CDS             complement(3137..3499)
                     /locus_tag="IW746_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079315.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3820)
                     /locus_tag="IW746_002437"
     CDS             complement(3503..3820)
                     /locus_tag="IW746_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079316.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEND010000159         4035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEND010000159 DACEND010000000
VERSION     DACEND010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.165"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(559..1506)
                     /gene="arcC"
                     /locus_tag="IW746_002438"
     CDS             complement(559..1506)
                     /gene="arcC"
                     /locus_tag="IW746_002438"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1079317.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(1621..2640)
                     /gene="argF"
                     /locus_tag="IW746_002439"
     CDS             complement(1621..2640)
                     /gene="argF"
                     /locus_tag="IW746_002439"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1079318.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(2731..3960)
                     /gene="arcA"
                     /locus_tag="IW746_002440"
     CDS             complement(2731..3960)
                     /gene="arcA"
                     /locus_tag="IW746_002440"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1079319.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1081 a          866 c          713 g         1375 t
ORIGIN      
        1 ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta
       61 attgctcgac ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga
      121 caagatcttt attattcatc tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa
      181 aaaatctcta gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta
      241 ccactccaat tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt
      301 ttaaaacaga attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt
      361 ccgaattcgc tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat
      421 taaactttct tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaaa
      481 aatccagatg gcctaaagga gaagaaatac cacctggata gctataaatc atttcgtaaa
      541 tacgaaagac ttaacaaatt aatcagctac gataatcgtt ccttcttcag aagctaaaag
      601 attctcgata ttttctaaag aagtgatgat tgccttagcg tttggttttg cttcaacaaa
      661 tgcaatagct gcttctactt tcggaagcat gcttcctgga gcgaattgct tttcatcaat
      721 gtattgtttc atttctgaaa cagtaacagt ttctaatttc ttttgatctg gtttttggta
      781 attgacatac acattgtcta ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc
      841 ggctaatttt tctgaagcaa aatctttatc gattacggct tctcgtcctt ctaatccagt
      901 agctgtttct acaacaggaa tcccaccgcc accaacagag actgtgataa ctccttgatc
      961 gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg ggtttagggg aagcgactac
     1021 ttttctccat ccacgtccag catcttcttt gaatgtcaca gtggagtcag cattcatttg
     1081 ttctttggct tcttcttccg tgtagaatgg accgactggt ttacttggat ttttgaatga
     1141 aggatcattt tcatccacaa taacttgagt gactaaagaa accacatctt tatcgatccc
     1201 tttttccttc agtacttcgc ccatggcgtt ttgtaaccag taaccgatag atccttctgt
     1261 cattgcaaca catgtatcta aaggcattgc cggtgttttc tcagaatcag cagcttgctg
     1321 ctgaatcaat aggttaccga cttgcggacc atttccgtgg gagatgatca attcatctcc
     1381 ttgttcgata aatttcacta aatactttgc tgtttccatt aacgcttctt gttgggcttt
     1441 tgcacttgca tccgtagaca agatggcatt gccccctaaa gcgacaacta cttttcgttt
     1501 ggccatttca ttcgtccttt cttcagttct accgaatttt ctagataaca aaacgagcca
     1561 gcgttttgtg attttccctc aatgctgact cgttttttcg tgagatgaat gtggttatgc
     1621 ttaaacgcga gggataaaca agttacctaa tgttgctgcc atgatcgctt tgattgagtg
     1681 catacgattt tctgcttggt caaattgacg agcatatttg ctgcggaaga cttcatctgt
     1741 tacttccatt tcagtgatgc caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt
     1801 atcatggaaa gctggcaagc agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc
     1861 taccatttcc atattgattt ggtaaggttt caacaatttg atacgttctt cgaatttatc
     1921 ttcttcaccc attgataccc atacatcaga ataaaggacg tctgctccgt ctacaccttt
     1981 atcaacatcg tcggtaatca tcaattgtgc accggatttt tcagcaaagc cttctgccat
     2041 tttgacgatt tcttcttcag gttgtaattc ttttggtgca acgatacgca tattcgctcc
     2101 taaaattgca cctgtgacta ataaagagtt agccatattg tttcgaccat ctccgcagta
     2161 agcaacagtg ataccttcta ctgttccaaa gttttcttgg atagttaaaa agtcagcgat
     2221 catttgtgtt ggatgccatt catcagtcaa gccattccaa actggaacac cagagaattc
     2281 tgctagttcc tctaccattt tttgactgaa tccacggaat tcgattccgt caaacatacg
     2341 tcctagtact ttagctgtat cttctgtgga ttctttttta cctaattgga tatcatttgc
     2401 acctagatat tcaggatgtg cacctagatc gatcgctgca gtagtaaatg cagaacgagt
     2461 acgtgtagat gttttttcaa ataaaagcgc aatatttttt ccttctagat agtgatgtgg
     2521 gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc
     2581 ttcttttgta aagtcttttt ctgctaataa gcttcttcct tggaatactg attctttcat
     2641 attaaacatt ctcctttaga ttgtttttat ttaagattaa agaggaaatc ccgacggcag
     2701 ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag tcttcacgga caagtggttg
     2761 gctcatgcag cgtggaccac cacgacctcg tgacaattca cttgaattga tttctaacac
     2821 tttgatgcca tagctacgaa gcaattcgtt cgatacataa tttcggttat aagtgactac
     2881 aacacctgga gcgattgcca atgtgtttga tccatcattc cattgttctc gaggtgcaac
     2941 gatctcatct ccattaccag ttgggatcag tacaagatca tctaatccaa gagcagcttt
     3001 catcgtttcg tgtaagttat ctgaatgagt aatcttgata tcatcaccat ctggttcgat
     3061 tgtaaagaca tccatcttac cgtctttact ttgaattgct ggatggatag tgaatttatc
     3121 gtgatctacc attgtaaata cggtatccaa atgcatcatt gcacggttgt tcggaatttt
     3181 gattgccaac actttttcaa atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc
     3241 taaagctttc gcattagttc gttgagaaat cccaacagct actactttat cgcttaagat
     3301 caattcgtct ccgccttcga tatgatctgg attttcacga tccaaccata cttcgattcc
     3361 tttatttgca aatcttggat gatgtttcaa aatatattct gtaaacatcg attcacgacg
     3421 acgtgcttca aaagtcatct tattgactgt catcccattt cccattgatg cggaagggtc
     3481 gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat ggatagtcat cgtctgctga
     3541 cagatcatat agactgctag aacgtacttc gatatctttt gtacgaaccc cagccatgat
     3601 tttatctacc atttcttgcg tttccattga tagaaggaac tcatgaagac cctctcttac
     3661 agcattcgat gcaatatgag attcatccaa cattttattc aagaattgtt ctttgacatt
     3721 gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa agagtctcga cgccttcatt
     3781 ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca atcggaagat atggaatatc
     3841 atcgaacaac agacgatcca taatatctgg tgtcaaattt tccacttctt gtccaggccg
     3901 tttcagtaaa actgtcttta atttccctat ttcagagaaa acgtgaatag gtttatccat
     3961 gttgtgttgc ctccttcttt ttgattacat gtataggata tcctcctttt gtaatcactt
     4021 tcataatttt gtctc
//
LOCUS       DACEND010000160         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEND010000160 DACEND010000000
VERSION     DACEND010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.166"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..1280
                     /locus_tag="IW746_002441"
     CDS             78..1280
                     /locus_tag="IW746_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAQ1079320.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2013..2981
                     /locus_tag="IW746_002442"
     CDS             2013..2981
                     /locus_tag="IW746_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1079321.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            2992..3807
                     /locus_tag="IW746_002443"
     CDS             2992..3807
                     /locus_tag="IW746_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ1079322.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
BASE COUNT         1331 a          575 c          752 g         1218 t
ORIGIN      
        1 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaagtgg caacattgtg
       61 tacttttctt tgcaatcttg tgtacaaaac tattgcaaaa cacagcgcat aagaatcctt
      121 acaaaacagt agatgaagca acccaattag atcttgttat agttgttaat cagctcttaa
      181 cgggttacga ttcaaaatgg gtcaacaccc tatacttaga taagtcactt gaatatcaga
      241 acataattca ggctttttca cgcacaaatc gtcttaatgg tcctgaaaaa ccgttcggaa
      301 ccattcgtta ttatcgtaaa gcacatacga tggaacagaa cattgaagag gctattgagt
      361 tatactcagg caaacaagca aaagggttat ttgttgaaaa attatataat aacttagaaa
      421 agttgaacga tatattcgaa gaaattaagt ccgtatttaa tatttctggc atagaaaact
      481 tcagtcattt accggaggtt ccaggggaaa ttaagcaatt tgtaaatctt tataatcaac
      541 taatccattg ttatgaatca gcgatgatac agggattcac atgggcaaaa tcgacttatg
      601 aaatccagga ttatgaacct ataacagtag taatcacaga atatgaattt aatatcttgg
      661 cacaacgtta cagcgagata gagagagaac ctagagacgg aaatgatagt atagcgtatg
      721 acattgatta ccaattacct gaaggtagtc gtagtcgtat taattatgag tatttaaaca
      781 caaacttcaa aaaatacatg aatataaaag aagaacgtga ttcgaattta attcaatcat
      841 tgttaaacga cttacatcgc acatttgcgc aattaccagt ggagttacag ccatatgctg
      901 agatgattct aaatgatgta aataatgggg aattagttat caaagaagga tgggaattta
      961 ctgattacct caatgaatat cagcttagtg aagaggacga ttttatccaa gatttagtgg
     1021 attcaaccaa tattaatgag gcattattac gtgagatgtt ggatttacgc ttaaccgagg
     1081 taaacattaa tgaatatagt cgatttgata aattaaaatc aagtgttaac gtaggtcatt
     1141 tcagtggata tatcgagaaa aatttaggta aaatggtaat accaatcaag gtaaatatgc
     1201 taattgacag attattgcgt gcatttttat tagaagatgt attcgatgtt acagagtaca
     1261 taaagaatta ctttaactag agtacaccta catcttatag tacataaaaa aattcatctt
     1321 ttttagttta ataattcacc aaaaaggtga gtaatgccat ttttgaaaat tgagcttgtt
     1381 ttatgtctac atctgtatcc gtatataatc caaaggatta taaactcaga agagtcttta
     1441 ttttttctga atataactac tgtatcaaaa acaatcgatt atgagacaaa acaaaatgac
     1501 ctcttttatt taagaaagga ggtcattttt tgtatcataa attgtattaa aatcaatgta
     1561 tcacttttta atcaatttta agaccataaa ttgcgtacta aaagaaattc tgcaattcaa
     1621 aataaagcat tttaagccat tttcaaattt aagtgcaccc atctacccgt caaatcactt
     1681 tattttccac tccagtagtt tcctaattca actgtgtgca aaacgatttc ttctaaacaa
     1741 atcaagacaa acttataaac cttcatccca gcaccataat tatttaaatc ataggcattt
     1801 ttcgaaatta gtatatgtac tcaagtttca ctgcagatta tttatatttt ttagagaaat
     1861 aaatttttcc tattgacaaa cacaaagaaa gcggatacaa tacaattatg tcacatgacg
     1921 ttatatgacg ttatgtaata tctttacatt taatcagtta cttttatttt ggataaaatt
     1981 aacattttta aaattttaag gaggaaatgt tgatggatgt agcaaaaacg gttaacgcaa
     2041 ttgtagaaaa aaatgatgga gagatttctg gaatttattt ggtggcttgt ggcggttcgc
     2101 tggtagatat gtatgtctca gattattttc ttaaagccga agcatcgaag tgtattacag
     2161 gcttatatac agcgaacgaa tttaaccatg taaccccaaa gcaattagat gataaatctg
     2221 ttgtcattct atgttctcat ggaggaaata cagcagaaac ggtaaaagca ggagaagtcg
     2281 ctcaagctag aggagctgtg actgttggtc tgactcataa taaaactgcg gatttattaa
     2341 aggtttctga tcatagtttc ctttacgaat ggggagatga tgcaaaaata gtcaataatc
     2401 ctatggccat cattcttgat ttaactgtat ctttggtcaa tgttaccgaa acatttgaac
     2461 attacggtaa attcagagaa ggcatgaagg ttatcgatac cgtaattgaa aatggcatta
     2521 agcaggtgca ggatcgtatt aaagtgtttg ctgataagta caaagatgaa acaatgtttt
     2581 atatcttagg tagtggacca tcatatggac atgcttatgg tttttcaatt tgctctttga
     2641 tggaaatgca atggcttgat tcaacttctg ttcattctgg agaattcttc catggacctt
     2701 ttgaagtaac agacaaagat acgaatttta ttctcttgat gagcattgga cgcactcgtg
     2761 ctttagatga acgggcaaaa cgattccttg ataaatatgc agaaagagta gaaatcgttg
     2821 atgcaaagga actaggattg gatatgatat cagatgaagt ttctgaattc ttcaatccca
     2881 ttttattcta cagtattctt tcagaatatc gttcagcttt agcagatgca cgcaatcatg
     2941 atttagatgt tcgtcgctat atggggaaag tggagtacta ggaggattaa aatgaaggta
     3001 ttaggcttag gtgataacgt cgtcgataaa tatgaaaatc tgggaattat gtatcctgga
     3061 ggaaattcat tgaattttgc agtttttgca aaaaaactgg gacatgaaag tagctttatg
     3121 ggagtttttg gctcagatga ggaggcagct catgttttat cagcaataaa aaaaatagga
     3181 ctggacaaca gtcactctcg ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt
     3241 gatgagggcg accggatttt ccttggcagc aattcgggag gtgttactcg tgaatacccg
     3301 attcagctta ctgaagaaga cagagaatat ttaaaccagt ttgaattgat tcatatggga
     3361 ttatatagcc atgtcaatca tctattagaa gagttacaaa atgtttctgg aaagatttct
     3421 tatgattttt ctgacgactt tacggaagaa gaggttcagc gggcaattga taaagttgat
     3481 tttggcttct tttctataga agacttttcc gatgaagaag taaagcagtt tttgatgaag
     3541 catttttgtg agcgaaatga tgtattgatt gcaacccgag gagaaaaaac ggctattggt
     3601 tatgatggag caaagtttta tgaagtgaac cccgtgctag aagaaccagt tgatacaatg
     3661 gcagcgggag attctttctt aacagctttc ctactttatt ttctcgaagg ggacgatata
     3721 gtcaccgcga tgaaaaaggg gaatgaattt gctgggaagt cttgtctggt tgacggttcg
     3781 ttcggctttg gtattcatta cgattgagct tgttttaaat tcctgcattt gataacctaa
     3841 agaaagaaat cccggtaaag tggaggttaa aaaaat
//
LOCUS       DACEND010000161         3817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEND010000161 DACEND010000000
VERSION     DACEND010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.167"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW746_002444"
     CDS             352..1041
                     /locus_tag="IW746_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1079323.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW746_002445"
     CDS             1028..2236
                     /locus_tag="IW746_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1079324.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW746_002446"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW746_002446"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1079325.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DACEND010000162         3816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEND010000162 DACEND010000000
VERSION     DACEND010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.168"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..771
                     /locus_tag="IW746_002447"
     CDS             <1..771
                     /locus_tag="IW746_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079326.1"
                     /translation="QEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKN
                     SNTGLEKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGT
                     KTIGVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVR
                     ENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS
                     EIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            795..1130
                     /locus_tag="IW746_002448"
     CDS             795..1130
                     /locus_tag="IW746_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079327.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            1127..1744
                     /locus_tag="IW746_002449"
     CDS             1127..1744
                     /locus_tag="IW746_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079328.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            1760..2227
                     /locus_tag="IW746_002450"
     CDS             1760..2227
                     /locus_tag="IW746_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079329.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            2243..>3816
                     /locus_tag="IW746_002451"
     CDS             2243..>3816
                     /locus_tag="IW746_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ1079330.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         1455 a          492 c          758 g         1111 t
ORIGIN      
        1 caagaagcta aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta
       61 gaagggcgta caggaactaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa
      121 gaagctaaaa actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct
      181 acaagtagtc aaggtggtcg atcagtagat gtccgagaaa atattcaagg ttcattgaat
      241 ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatggtcaa
      301 ggtactaaga caattggtgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa
      361 acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg
      421 gatgttcgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga
      481 gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac
      541 gttcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac
      601 agaaagtcag cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc
      661 cgtgaaaatg ataagggatc aaaaccaaga cgaagattca cttatgaaga caacgaatta
      721 ttcagagata cacttaatga tcctaatcca cttttcgata atttaaatta aattaaaagc
      781 cggcatgagt gattatgccg gctttttatg aggtgaaaat gatgtataga aatcccaaaa
      841 atataaaaca agagataaag ctatttttct tctatttagt cgatttagga atagtagccg
      901 gaagcgtgat ggcaagtgtt tactttgcaa aattaatgcc aatttcacca atgatgaaat
      961 ttgcactgta cttattagca tttgtagtgg gtgtttggat gtgcgttaga acgcctaatc
     1021 accctaaaga gcgaaatatt ttaatgctgt attatctctt taaaatggat agaaacagat
     1081 accacgaagt agagtttaaa agaaatagta gggggaaaat tgaattatga ttgttgctga
     1141 aagtaatgaa aataaattaa gtggtgtttt tgacaaattc ttagaactag ttattccaaa
     1201 agataaagat atgtgtgaac ttttaccata taaaaaaata ttgaaagacg gaacgttagt
     1261 gactaatgac aataagtatc aagccttttt taaagttaaa acaactgact taacttctat
     1321 ttcagaagat ggatttatgc gtatggtaaa caaactcact tatttaaatc gagtatattt
     1381 agaaccatat aaaatttatt cattaactta tcctactgaa acagtaaatc aagcgattta
     1441 ttggaagcat agaatacagg aggcaaagag aagtttattg agtaataatg atcccaattt
     1501 agaaacgtat ctgaagtgga atctaaagct agcttacgat aattatgaaa gagttgatct
     1561 agtatcgact ttaccagagt tgatgttcgt tatttgtgtt tatggaaaaa cacaaaaaga
     1621 agtgaaaaat catgccgatg taatgaaaca ggccggtggt agagaattag atttacaaag
     1681 aattactgaa ggggatttaa tcaatgtgat aactaaattg atgaatccca atacttcttt
     1741 ataaggcggt gcttattatt tgttaaagca ctgtagaaaa ggattttgta aagtaattaa
     1801 aggtaaaaat gctgctgatt taatctttga atggaaagaa tctaatttaa ttaatgtacg
     1861 tgggaaaaat ggaagcggaa agacagaaag agtgatggct agtattgata attcaaggcc
     1921 tagatcaaga gcattagtaa taactgatga aaaaaatcac tataaaggca tacgaacgat
     1981 agaaaaaaaa tatttaacta acaatgattt aaaagtagta gatgatattt taaatattta
     2041 tcctgatttt aatttagttg tttttgatga aacaattcct gtgacagaaa taaaaggatt
     2101 ctatcaaagc ttttccaatc tagcaaataa atatgaggaa gttctgtttg tttggattac
     2161 agaagaagat agtaatagca agtcagcgtt ctttagtgaa aagaacgctt ttttacattc
     2221 tatttaaaaa ggagaattga acatgattgt aagtaaaaaa ctagcaagaa atctagtaag
     2281 tgaagggtat gacttaagat tagtagaaaa gtatcaaccg agcggtggaa ttaaatttga
     2341 tgagcgttat atttctactg gtgatggata tgtaacttcg attgctgtta ttgagtttgc
     2401 agaagatgta aatagtcgat ggttaacgga tataatggca attccaaata ctattgcaac
     2461 tttcgatgta tgtacggcgg ataaagaaga cttattacac aaaatcaata cgtctattgg
     2521 agaattagat taccgatcaa caaatgagaa tagaacaact gacaaaacag acgctttact
     2581 agataaatac aatttagaag attttgcaca agaaattacg aaacgtggtg aagttgtcaa
     2641 aactgttcat gctcgtattt ttgtttatag cgattcttta gaagaattag agaaaagaat
     2701 tagtgagatt cgtaaagatt tggctagtgt caatcacaag gcaattgttc cgcaatttag
     2761 aatgaaggat gaatggatgt ctttatttca agatggcatg gaacaagaat caaaattatt
     2821 tgccaagaaa ggcttaactg ttccggcaaa aaatattggt ggcggtattc catttcacca
     2881 caagagtttg aaagatccac gtgggtttta tttaggaact acgtcaactg gtggtgcttt
     2941 tatatttgat ccattttatt ctacgaagca aagacggtcg tttaacggct ttattttagg
     3001 aaagatgggt tttggtaagt caactttaat taaacagcta caggaaggcc tacaagcgaa
     3061 aaattgtttt attcgaggat ttgacaagtc aggtgaattt gaaggcatgg tgaagaaagc
     3121 aggcggtgta tacgtcgatt taggcggtgg tgatatggta gtaaatccat tgcaaattta
     3181 tgcaacagtc atgaaaaaag atcaatttgg aaatgatgtt gtagatgaat caaagagttt
     3241 tcaaaatcat atcactaagt gtagtaatca aattcgcttt ttagaacctg aaattgatac
     3301 ggcaactgta cgtgaatata ctttgatgtt acaagacttt tatattcatt atggaatttt
     3361 gccaaaagaa tggagagaag cgagagacga caacgaacga aatcaattaa atacaaatat
     3421 ttgtaacttg cctaatgatt catatccaat ctatggagag tttcttaaat ttattgaaaa
     3481 tcaacaattc aaaattaaaa atgctactcc tgaacgtgtc cgttctattg agctaattaa
     3541 gcttatcgtt acggatatgt gtacaactta tccgcaaatt ttcaacggag taacaacaat
     3601 gccaaacttt gatacaacaa aaattgttta ctataacatt gattctatta gtaaaatgag
     3661 tgccccagta ttccattgcc aattatttac agcgttaacg actatttgga acagtgcctt
     3721 gcaaaacgga aagattcaaa aagaaaactt taaaaaatat ggcagtgatt attataagta
     3781 tgcacaagat ttcatggttt tgatcgatga gtgtca
//
LOCUS       DACEND010000163         3708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEND010000163 DACEND010000000
VERSION     DACEND010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.169"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            329..934
                     /locus_tag="IW746_002452"
     CDS             329..934
                     /locus_tag="IW746_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAQ1079331.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            950..1525
                     /locus_tag="IW746_002453"
     CDS             950..1525
                     /locus_tag="IW746_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079332.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            1685..1888
                     /locus_tag="IW746_002454"
     CDS             1685..1888
                     /locus_tag="IW746_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1079333.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            1997..3322
                     /locus_tag="IW746_002455"
     CDS             1997..3322
                     /locus_tag="IW746_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1079334.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            3315..3665
                     /locus_tag="IW746_002456"
     CDS             3315..3665
                     /locus_tag="IW746_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079335.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1331 a          576 c          774 g         1027 t
ORIGIN      
        1 tttaattatt actaatactc aaaataaaaa cagggtatgc tactgaaaaa taaagaaaca
       61 cctgatttta tgaacacttt gttttaaaat aagaactgaa aaaatcactc aaaataattg
      121 agtgattttc ttagcgatta aatgtttata gtttgtgccg aataaaataa aaaatttttg
      181 ccatttaaaa agaaaaggac agatggcttc tattgaaaac taactacgtg tcaaaaactt
      241 aatagttttt gacacatggt taaattcgcg atacaatagg atttgtagtg tttcataaac
      301 ttatgtcaaa aagttaaggg gattttccat gattttagaa aataaattgg gtttaacgaa
      361 tcaggttgag ttggccaagg ctgaagaaaa actcagtaag caaaaagcaa aagaattata
      421 tgattctgga aaaataaatg aaatcgaagt cggcacgttt aaaggcttgt ctgaaattca
      481 tgattttctc ttttcagaaa tttatgattt tgcaggaaaa attcggacag ttaatattgc
      541 aaaaggaaat tttcgttttg cgccggtcat gtacctagaa caatctttaa atcatattga
      601 tcagatgccg caaacttctt tcgaagagat tattaagaaa tacgtggaaa tgaatatcgc
      661 tcaccctttt cgcgaaggaa acgggcgcag tactagaatt tggctggatc tcattttaaa
      721 ggaagaattg caaaaagtgg tcgattggaa cttgatcgat aaagcagact atctttctgc
      781 aatggagcgc agtccaataa atgacttgga aatacgctat ctcatttcca atgccctgac
      841 tgataaaatt gacgatcgtg agttatatat gaaaggaatc gatgtcagct acttctatga
      901 aggatactct gagtatacga tagatgattt gtaaaagcag gtgagttaaa tgacaaaata
      961 tggctatgca cgtgtctcaa ccgcttctca aagtacaaag gaacagatac aacagctact
     1021 tgagaacggt gttgagaaga agaatatttt ttctgaaaaa tttacaggta cgaaacgaga
     1081 tcggccaaag tttaatcagc tgttggctgg tctgaagcct ggagacgaaa tcgttgttac
     1141 gaaacttgat cgattcgccc gtaatacaag agaagcacta gagatcattg aacctttact
     1201 cgatgataac gtcacgatta aggtattgaa tctagggacg atcgaaaata catcgatggg
     1261 tcgaatggtt acacgtacgt tgttgagtgt agctgaaatg gaacgtgata tgattgtaga
     1321 acgtactcag gaagggaaat tatttgcgaa aaagaataat cctaatttta aagagggacg
     1381 tccgaaagca acaatcacac caaagaagag gcatgcatat gaattgatta cttccggcaa
     1441 atcgtataaa gaagtggaag ccataacggg tttcagtcgt agtacacttt ttagaattaa
     1501 gaaacaaata gaagcgagtg agtagaagat tgataaaatt aatataaaaa tctcttttaa
     1561 atcaaataaa tcgttgttta tacgagcatt ctaaggtcag gttcactcat taagatgtat
     1621 cattggtcga acgaagcgtc ataccagcta cgaatagaca tatttttagg gaggaacgaa
     1681 gtaaatgaaa gaacaacaat taggctacac tctttgggga cgtccattat acggattaga
     1741 acgaagaacg gctgtatcga ttgactgtgc ttgtggtgag ttgattcaac tcaatattca
     1801 aacgaaacaa tacaattgta aaaattgtgg gaattgttac caattcattg atcaagactt
     1861 ccaattgcta aacgaaaaaa acaaataggt ttattatagt cagacaattg tctggctatt
     1921 tttctattta gagaagatcc tcgttgcaaa cacgaacgta tgtttgtata atatgagaga
     1981 agagggggag aactttatgc ttgattactc aaaagagcca gtcaatgact actttctaat
     2041 cgatatgaaa agtttttacg caagtgtcga atgtatagaa agaaatttag atccattaac
     2101 gactgaatta gttgtcatga gtcgagcgga taatacagga tcagggttga ttttagcttc
     2161 ttcaccagct gcaaagaaga aatatggtat ttctaatgtc agcagaccac gggatctacc
     2221 acagcctttc cctaaaacat tacatgttgt tccaccacgt atgaaccttt atattaagcg
     2281 taatatgcag gtaaataata ttttcagaag atatgtggct gatgaagatc tactgattta
     2341 ctcgatcgat gaatcaatct taaaagtgac ccgatcactg aatcttttta cgactgaagg
     2401 aacacgaagt cagcgaagga agaatctcgc tcaaatgatc caagaacgta ttaaagaaga
     2461 gctaggattg attgctacag taggtgttgg cgataatcct cttttggcta aactagcctt
     2521 agacaatgaa gcgaagcata atgaaggatt tattgccgaa tggacgtatc aaaatgttcc
     2581 tgataaagta tggaatattc ctgaaatgac agacttttgg ggtattggtt ctcgaatgaa
     2641 aaaaaggctc aaccaaatgg ggatcatgag tatcaaagat ttagctaatt ggaatccata
     2701 caccattaag agccgtttag gtgtgattgg gttacagctg tacttccatg caaatggtat
     2761 tgatcgaact gacatagcaa tcgcaccaga accgacaaag gaaaaatcat atggcaattc
     2821 acaagtgcta ccacgagatt atactagaag aaatgaaatc gaactggtcg taaaagaaat
     2881 ggctgagcaa gtagcaatcc gtattaggca acaccactgt aaaacaggtt gtgttcattt
     2941 acacattgga acgtcgattt tagaaacgag accagggttt tcacaccaga tgaagatatc
     3001 tatcacagat aatacaagag agctacaaga ctattgtctg ttcttattta ataaatacta
     3061 tgagggacaa gaagtccgcc atgtcggaat cacttacagc aagctagttt atactgattc
     3121 gcttcagctg gatttgttta gtgatccaca aaaacagatt gatgacgaaa aactagacaa
     3181 aataatagat aaaattaggc agaagtatgg ttttacgtca attgtacatg cttcttcgat
     3241 gttagaaggc gctcgtagta ttactagaag cacgttggtt ggtggccatg caggcggcgc
     3301 aggggggctt aaaaatgatt gactataacg atcgaggacg attgaaatgg gcaggtttct
     3361 atttatcgga tcatactgaa gagatcgatg cagataatga gcaacgtgca aatcaaaatt
     3421 tagcaaagga acaaatgagg actgaagaga ttgcggaaat attgaacgct tcagtcataa
     3481 aaaatagatc agtatgtatt caaaaagaag aacgaaacgc tgaaggatac tatccagacg
     3541 atattgttgg aaagatctta ggtagtgatg agctgggaat ctacgttggc aaagacaaaa
     3601 ttcactacga cgaaatacgt catatcgaat tttaccagga taataaatgg agtcgattag
     3661 aatgaacatt atccagcaat atgagctaaa gtatataacc tttgatca
//
LOCUS       DACEND010000164         3632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEND010000164 DACEND010000000
VERSION     DACEND010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.170"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(560..1600)
                     /locus_tag="IW746_002457"
     CDS             complement(560..1600)
                     /locus_tag="IW746_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1079336.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IW746_002458"
     CDS             complement(1949..2275)
                     /locus_tag="IW746_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079337.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IW746_002459"
     CDS             complement(2262..3050)
                     /locus_tag="IW746_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1079338.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1108 a          600 c          507 g         1417 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa tt
//
LOCUS       DACEND010000165         3625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEND010000165 DACEND010000000
VERSION     DACEND010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.171"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..244
                     /locus_tag="IW746_002460"
     CDS             <1..244
                     /locus_tag="IW746_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079339.1"
                     /translation="MTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVD
                     RAVKLYKSQVYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(523..810)
                     /locus_tag="IW746_002461"
     CDS             complement(523..810)
                     /locus_tag="IW746_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1079340.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(800..1129)
                     /locus_tag="IW746_002462"
     CDS             complement(800..1129)
                     /locus_tag="IW746_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1079341.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            1434..1763
                     /locus_tag="IW746_002463"
     CDS             1434..1763
                     /locus_tag="IW746_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079342.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2050..2535)
                     /gene="vanZ"
                     /locus_tag="IW746_002464"
     CDS             complement(2050..2535)
                     /gene="vanZ"
                     /locus_tag="IW746_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1079343.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2688..3599)
                     /locus_tag="IW746_002465"
     CDS             complement(2688..3599)
                     /locus_tag="IW746_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1079344.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1185 a          619 c          536 g         1285 t
ORIGIN      
        1 gatgactgtc ttccaagcat ttagtcagtt cgaacgtgat ttaattgttg aacgtaccaa
       61 agaaggtttg aaaagtgcac gtgcgagagg tcgtaaaggt ggccgtccac gagtgaacca
      121 gaaagatgtt gatcgagcag taaaattgta taaaagtcag gtttatagcg taaaagaaat
      181 tacggaaatg actggtatta gcaaggcaac actctaccgc tacttaaaag acaataggga
      241 ataatctatt ttaatcttta ggaatctata atatttatgg gagaattaga gtgatttttt
      301 tattattact aaaaaacaat ataaaataac gcattttaag ccctctttgt gcataaggat
      361 gaagctttta atttgaaaga ggcttaaaat gcgttttaat tttttctatt tttcataaat
      421 atataggtta gtggtttaaa attatagggt attacaactc tcagctgtta aaaattatct
      481 tttaaagtgt cgtctatcct ctcaagttga aacattgggt tcttacctaa tcttttttaa
      541 ttgttcataa aaattttcat gcgttccaca tagtaagata ggtatcactg cattatcttt
      601 aatttcatat gcaacgcgat atgtcgtacc tgcatacttg aatccaatag cccaaattcc
      661 tgataaatca cccgtttttt gttctcccga atgaggacga actgctatct catcataaat
      721 tatcgttaaa aattgttctt ttaatgtttt ctcttttaat ttttttagat actttattac
      781 gtgtttagaa ggtagaatct cataagccaa tttctgctgc caactcctct tttgtcatcg
      841 gtttagaatt tgcaacagta tcttcagcaa tagatttaaa tgcagaagtt aacgcatttt
      901 ttctatttct aaattctttt aagatctctt gtttgctata accttctgaa agaatatcag
      961 ccaaaatatc ttcactaaaa tcaaaaaatt cttttttagg ctctacaaac tcataaagga
     1021 taccattctc tacaagctta gcttctactt caacaccatt gggcacatcg aattctttag
     1081 ggaccgttaa cataatagaa tttccttgtt ttcttgtttt tgtaatcata ttatttcctc
     1141 cattatttta catttcgtaa ttacttagta attacattgt acgctatttt tagtagaaat
     1201 acaaaataat aaaactcctt tagaacaatt ttcaaaggag ttttatttga ttaggaaatt
     1261 atgtagaata ataatatgtt tttaacttgg aatcaaaaac gcacttgcaa gtaagtttac
     1321 ttcaatacat gaaattgctt ataatagtgt attggaatta aggtagattg acaaaagagt
     1381 gatcaaatag gtatacttca ttcaaataat cgtttgaatg tgaggtgaga tgaatgaaaa
     1441 aagtttgcca agtgactgtc gtacataaag aaaaagtaaa tggaataaaa aataaattgg
     1501 atcaaaaaga tttttcaaac ttacttgtgt tagggaaatg tttcagcgat ttttctagaa
     1561 ttaaaatttt ctatgcttta gaaacctata aggaaatgtg cgtctgtgat ttagcagaaa
     1621 tacttactgc tagtgtcgct acaacgtcac accatttacg ttttttaaaa aaacatggaa
     1681 tggcaaaatc acgtcaagat ggaaaagtag tttattactc tttagcgaat gaagacatcc
     1741 tttccgctat tcgtgttttt taaagttatc agaaaatcta tctatgaaat cttagtttgg
     1801 gggtaccgtc aggaaaatgc ggatttacaa cgttaaggaa atttattaaa gagaccaatt
     1861 cctcagcact aaaattgata attgggcttt ctttgttact atagtgttag gtaagctttt
     1921 tactttatca ccggttttcc cctcacttca cacctacggg cgagtttccc cacatcccgt
     1981 tttccatcta atccaattca ttcaatatct ttatgtgtat gaaagatcag attgcttgat
     2041 ttagacatct tacacgtaat ttattcttaa atgggtacgg taaacgagca ataatacgag
     2101 caaaagtata cctacaaaaa taataactct gtctaatttt ttttgattca tatgcttatt
     2161 gcttaaacca tataatttca gtccaagaaa gcctccaaca gtatttgtaa ttacatctgt
     2221 tatgtctgtc gctccaatag cgaagataaa ttgaattatt tcaaaagtaa gacttaaaac
     2281 cagtacaaaa gcaaacttag gtaaaaatcc gatttcttta aaattgacat tcaaaagcaa
     2341 gccaaatgga ataaagatta taacattatc tatcatctct ctgaaattcc cagtagcagt
     2401 aaatggagtc aagttaagac ttctttgatg ataattaaat actgataaaa tattgtattg
     2461 taatttgaat aacactaacc agattagtgt cactaaatat aaagctagca atcctctaga
     2521 taatattttt cccaatataa tttcctctaa ttcttttgtt taagttgtat cattagtata
     2581 gcaatgtcct agttaacgtg ggttttaaat taaaaagcta acgttacccc aaaagtgcta
     2641 gtacaataat gctgttcatt aatttcgttg gtttcattcc gccatcctta cctccttgaa
     2701 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
     2761 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
     2821 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
     2881 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
     2941 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
     3001 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
     3061 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
     3121 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
     3181 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
     3241 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
     3301 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
     3361 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
     3421 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
     3481 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
     3541 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
     3601 ttcagtctcc tccttaacta tatag
//
LOCUS       DACEND010000166         3546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEND010000166 DACEND010000000
VERSION     DACEND010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.172"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1615)
                     /locus_tag="IW746_002466"
     CDS             complement(83..1615)
                     /locus_tag="IW746_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1079345.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            1849..2550
                     /gene="nagB"
                     /locus_tag="IW746_002467"
     CDS             1849..2550
                     /gene="nagB"
                     /locus_tag="IW746_002467"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1079346.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            2804..3517
                     /locus_tag="IW746_002468"
     CDS             2804..3517
                     /locus_tag="IW746_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1079347.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         1065 a          623 c          731 g         1127 t
ORIGIN      
        1 attggccacc atttattcag aagaaggagt tgtgacaaaa cttttgtcac agctcctttt
       61 tttcaactat tcaatcaaac gtttattttg gttcaatctc aatgactcgt tgcacaagat
      121 cttgataaaa ttcttgttca tgagagacga tgattactgt tcctggaaat ttctgtatcc
      181 cgtcttgtaa gctttctttc gtttcttgat cgatatggtt cgtcggttca tcgagaatca
      241 gtaaattacc ttggttcatt gtcaactgtg caagtttcac cttagtttgt tccccaccac
      301 ttaatgtagt gagaggttca cttgctaatt gattaactaa ccctgcttta gctagttggc
      361 gacgtaattc tttgatagtc gcttttggaa attgactgct taaatattgt agtggtgttt
      421 ctaatggatt ttcccagtac agatcttgtg aaaagtaatt gatttttgtg ttatctggga
      481 agtggaaatc gccacttatt ttttcaatct caccgatcag tgtttttaat aaagtagatt
      541 ttccaatccc gttgaagcct cgaatggcaa tattttcacc aaagcggatg gtcaaattga
      601 tgggtgcaag tagcggtcga tcatatccga tgactaaatc agttgtttcc actgctaatg
      661 tcgtgacaat aggcgagaat ggaaaatcaa agacagcttt acttgctgaa gctggaggcg
      721 tcagccgttc gattttatct aattgttttt gtcggctttt tgccatcgtt gatcgggaac
      781 cggctttgta ttttcgaata taagcttcgg tcttttcgat ttgtttttgt tgagcatgat
      841 attggcgcaa atagctttcg cgattttgtt ccttttgttt caatgcttgt tttagatggc
      901 cagtatactt agtaatcttt ccaaactcga tgtctgcgat atgggtggtg atctgatcaa
      961 gaaattcttg gtcatgggaa atcacgatat acgcaccgtc aaaatcctga agaaactgcg
     1021 tcagccattg gatatgctga tcatctaagt gattggttgg ttcatctaag atcagaacat
     1081 ctgggttttc taaaagtagt tttgctaaaa tcacctttga acgttgtcca ccacttagat
     1141 ttttcaacag tgaatctaaa cctaagacat cgatccccaa cccgctgctc atctcagcaa
     1201 ctaaggtgtc gatttgataa aacactcctt ggtctaattc cgtttggagt ttcccagctt
     1261 tttccagcaa ggaatcatcg aaggtctcgc tatatgtttg gtaaagaaaa gcaatttttt
     1321 cttccttttc gtattctttt gcgtacgcag tttttagaaa ttcttcgatt gttaatgttt
     1381 catcgacttc cacatattga tcgaggtaac ctttgtgaat atttttttgc caatggatct
     1441 gtccgtcatc tggtaagatc tcaccagtga tgattttaat caatgtactt ttaccggctc
     1501 cgttttgtcc aatcagccca agatgttcgc cagcatttaa ttgcagatcg acaccttcat
     1561 agaggatttt gtcgccaaat tgctgggtaa tgttgtttaa tgtcagtata ctcataataa
     1621 ctcctttttc ataaaaaaac tgcagacata aagcgtccac agtgaatcac ttgcttggtt
     1681 ttcatgcaac atggattgtt tgattgaacc atcttatgaa acctaacacg gattcctatt
     1741 tattgtcaag aaaatgaaaa aaatgcaatt tggtctatac taattattgt aaaaagaaaa
     1801 aaagaatgct ataattgggc tataccaaaa tataaaggag caatcactat ggaaatcatt
     1861 cgtgtaaaaa acgctgaaga aggcggaaaa aaagcatttg aaatcatcaa agaaggtatg
     1921 aacaaaggtg caaaagtatt aggattggca acaggaagca caccagttac tttatataaa
     1981 gaaatgactt caagcgattt ggatttcagt gaaatgactt ctgtaaactt agatgagtac
     2041 gttggactag gcggttcaga tgaacaaagc taccgtcatt tcatggatgt ccaattattt
     2101 gataaaaaac cattcaaaga aacgtttgta ccagatggaa aagcagaaga tctggaagca
     2161 gcatgcaaaa agtatgatca aatcatcgat gcacatccaa tcgatatcca aatcttagga
     2221 atcgggcaaa atggacatat cggtttcaat gaacctggtg cttcatttga cggaacgact
     2281 tcagtagttg acttaacaga atcaacaatc aatgcaaaca aacgattctt cgataaagca
     2341 gaagatgtac caacaaaagc tgtttcaatg ggaatcggtt caattatgaa aagtaaagaa
     2401 atcgtcttga tggcatttgg cgaaagcaaa gctgatgcaa tcaaagggat gattgaagga
     2461 cctgtaacga atcatttgcc agcaagtgta ttgcaaaatc acgacaatgt tgttgtgatc
     2521 atcgatgaag aagcagcaag taagttgtaa ttttcaatag taggctaaag aggttgtgaa
     2581 atcagcgttt tgacctgagt attagagaag acaaaggaaa aatagctccc catattttgc
     2641 cgtttgttga gtaataccga gggtcagctg attgaacacc gtttattcat aaaaaggact
     2701 atgacaaaaa ttttgtcaca gtcctttttt cgtaagaggt aaaaattgca ctttttttgt
     2761 ctgctccttt cagacatgat atattggact ggagggtgtt tagatgaatg atatggtatt
     2821 gacaattcca gagattattt tacgtttagg gatcgctatg ctatttggcg gagcaatcgg
     2881 ttttgaacga caatataaaa atcgtccagc aggtatgcgt acgcatattt tagtttgtat
     2941 gggagcatgt atcattgcat tgatccaagt tgagatcgct agtggtgcat taagagatgc
     3001 tttagagtat ccacagcttg ctggaacgat tcgttcagat gaagcaaggc tgattgctca
     3061 agtggtgagt ggtgtcggtt tcttaggcgc gggaacgatc atcgtcacga aacaatcggt
     3121 gacgggactt acgactgccg cttctttatg ggcaattgct ggattaggga tcgccatcgg
     3181 catgggttac tattccattg cgatcatcag ttttatcggg attgctattg cactcacagt
     3241 agtgaaacgc gtgatccatg tacctacgac taaaaagtta gaaatccaat ttatccatag
     3301 aaaagaaacc aaagattttc ttaccgatta ttttgaagag aaaaatatcg taatcgaaga
     3361 tgtgaatttt gacgtaaaat tcgttgatga ctaccgagtg tacaaaaata tctatacgat
     3421 tgatcttcca aaagggctca cttatgccga tgtgatcgag gagttgtccg tacacaaaaa
     3481 tatcacaaag ttgcatttag tcagtattgc gcaataaatg atctgaggtt gtaaaataga
     3541 tgtcct
//
LOCUS       DACEND010000167         3462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEND010000167 DACEND010000000
VERSION     DACEND010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.173"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW746_002469"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW746_002469"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1079348.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW746_002470"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW746_002470"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1079349.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW746_002471"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW746_002471"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1079350.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW746_002472"
     CDS             complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW746_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1079351.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          991 a          757 c          594 g         1120 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aa
//
LOCUS       DACEND010000168         3429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEND010000168 DACEND010000000
VERSION     DACEND010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.174"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(224..709)
                     /locus_tag="IW746_002473"
     CDS             complement(224..709)
                     /locus_tag="IW746_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1079352.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1189..1830
                     /locus_tag="IW746_002474"
     CDS             1189..1830
                     /locus_tag="IW746_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1079353.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1849..2484
                     /locus_tag="IW746_002475"
     CDS             1849..2484
                     /locus_tag="IW746_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079354.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2502..3317
                     /locus_tag="IW746_002476"
     CDS             2502..3317
                     /locus_tag="IW746_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1079355.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1115 a          565 c          733 g         1016 t
ORIGIN      
        1 aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat
       61 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
      121 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
      181 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
      241 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
      301 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
      361 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
      421 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
      481 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
      541 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
      601 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
      661 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
      721 ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa
      781 ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag
      841 agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat
      901 tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga
      961 tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat
     1021 taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg
     1081 tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct
     1141 cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg
     1201 attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt
     1261 accttgattg gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt
     1321 aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg
     1381 ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc
     1441 gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt
     1501 ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt
     1561 ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct
     1621 gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta
     1681 ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg
     1741 ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa
     1801 gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg
     1861 aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt
     1921 ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta
     1981 cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg
     2041 ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc
     2101 cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg
     2161 aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt
     2221 agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg
     2281 attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct
     2341 ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg
     2401 gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta
     2461 ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc
     2521 tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag
     2581 cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat
     2641 ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag
     2701 ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa
     2761 tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa
     2821 cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc
     2881 tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag
     2941 gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag
     3001 agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc
     3061 tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga
     3121 aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt
     3181 ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag
     3241 atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg
     3301 ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt
     3361 ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac
     3421 aacctcttg
//
LOCUS       DACEND010000169         3412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEND010000169 DACEND010000000
VERSION     DACEND010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.175"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..260
                     /locus_tag="IW746_002477"
     CDS             <1..260
                     /locus_tag="IW746_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1079356.1"
                     /translation="LDGREIQLIFTAKTPLQSEKNIDNQAVVAVDNVSNKTNVVTIGV
                     DPNLPQVIVPKTGSTHLVTILAVSLLLLVLATFGYAVLKFN"
     gene            complement(296..2353)
                     /locus_tag="IW746_002478"
     CDS             complement(296..2353)
                     /locus_tag="IW746_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1079357.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2497..2688)
                     /locus_tag="IW746_002479"
     CDS             complement(2497..2688)
                     /locus_tag="IW746_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079358.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            2876..3331
                     /locus_tag="IW746_002480"
     CDS             2876..3331
                     /locus_tag="IW746_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079359.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
BASE COUNT         1032 a          563 c          669 g         1148 t
ORIGIN      
        1 ttttagatgg acgagaaatt cagctgatat ttacagctaa aactccatta caatcagaaa
       61 aaaatattga taaccaagcc gtagtagcag tagataatgt ttctaataaa acaaatgttg
      121 tgacaattgg ggttgatcct aacttgccac aagttattgt tcctaaaaca ggttctacac
      181 atttagtaac gattttagcg gtcagcttac tattacttgt gttagcaact tttggctacg
      241 cagttctgaa gttcaattaa acaaaaaaac aaggctgaaa atcagccttg tttttctatt
      301 tgaaaatggg attggcacta ataaatacgt tgtctgaaga aagtgatccg tctgcagaaa
      361 cttcataagc atgggataaa ccaacgatac tttgattgtt gtaaagcaag tttgcgttat
      421 gagcagcgct atctttccac ataaccatag catttactgc tcctgtatct gaaaggatct
      481 cgtcaacgta aaggtagaaa ctattgatat tttctccttt agcaccgtag cagcctaagc
      541 tttctaagag atcttggaaa gtaccgccgc caggtcggta atgactgaag cttacttgta
      601 attcttttgc acgaatatct gcagcttgtt gaacgatatc attgaattgc attggggcaa
      661 cgccattttg ttgacggtag ctatttaacc aagcaagttc tttttgacgc acatagttgt
      721 ttaaatcact tagtgagata tttttgtatt tgaaagtgat cgttcgattt tggtcaacat
      781 tattgattac acgttgtcca tttttatcaa cggtaacaga tgaatcgtca tttctacaca
      841 agaagtagtt ttgatcggtc atatttttcc aagaagtaga gaaactatct ccctcgtaaa
      901 ccgtttgtgt ttcagtagat aaaacagcgt ttgtgtcaac gtttatatgg ttcacagtga
      961 cgttgtattg atgagtatag gcaaaattga ttccttgatt acttgtaacg ttgtttaaag
     1021 tcacagtgtt gctagagctt tctctaggat caagaacaaa attagtgaac ataggtgctt
     1081 caattgtgat gctttgtcca taaacgacgg gataactctt actgtaatta tagagttctt
     1141 ggatatttgg atcagtatcc gagactttat ttggacgagc tttcacttca actagaaaga
     1201 cttcatcata gtaaaaagta aatgtggtat ttgtgttaat cgtttgtgtt tgtgtttcat
     1261 tcccatttac ttgatactgt ggtgcttcaa agttagtcaa gtcggttcta gcttttgcag
     1321 tgtaacttgt accagctttt actgagacat gttcttcttt tagcagcgtt ccagtttgtc
     1381 gattcatatg acggacagtt accgcatatt ctgaaggttt aggtttgtgt tgtaaccctt
     1441 taaatgcaga atcaagacta tttttagcat tatttacttg ggtttgagaa gctttagaat
     1501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gatccttctg
     1561 taaaatctcc tttagcagtt gattttactt ggttgtacaa ggcttgtaat tccgttttgt
     1621 tcacagtagg agctggtttt ttctttaatc ccttgtaagc agaatctaat gtgtttttgg
     1681 cactatccac ttgtttttga gtcgcttttg agtcatttaa cactttttta gcattgttta
     1741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc acctttttta gttcccttaa
     1801 cgctcgtata aagagcttct aaagctgatt tattcacttt gggtttagag gtgtcagcac
     1861 cataccaacc aataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
     1921 tccaacttgc agtgtagtga tgcgaaccag aggtaacacc tggaaggaaa aggcggtaga
     1981 tcggtttatt atttttttcg gcagaacgcc atgcaggccc ttcataagtc caaccgtaat
     2041 ttttggtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaaggcctt
     2101 tattgtataa acgatagaca aggtggccac cgctagtcgg tgcttcccaa gcggggcctt
     2161 cataattacc ccacttaatt ttcactaggt gatcacgttc ttttgtgcta gaggtgtaaa
     2221 aatgttcatg gttatttgga ttataaaggc gatacatgac gttagttgaa gcagcgtcag
     2281 cagatccagc aagagcaact gtggcagcaa atgttccaat agctataaca gtttttgggt
     2341 ttaataattt catgttttca actcctatta aatgattaaa tattttgttt acattcataa
     2401 tataccacgg tataccgagg tagtcaacgg tataccgtgg aaattttgaa ataaaaaagc
     2461 cgccctatga agagtgtaag gatggctgtt tatatgctat gaagaaatgg attcggcgta
     2521 tttggttagt ttggcagcat tatcaaatcg cattttttca atcgcacttt ttttctttat
     2581 taaatccgca attgttgatt ctgcgactga agaatgtttg ctaatttcat atgctgtctt
     2641 gccagtttca aatagtaacc attcgatttt ctctttatca gcgtacattg caatgctccc
     2701 tcttgatttt ttctttatta tagctctttt ttgtaataaa tcacaatatc aagaggaaaa
     2761 gtttctatag aaaggtggga aattatgagt atccgttccc cgtgtatcaa aatgaggttt
     2821 gttgctactt tcgttatttg atatactaaa ataaaacaac gaaagaagga atagaatgaa
     2881 aaagttaata atggtattta cttcgttgat tgccatgatt gttattttag gtggatgtgg
     2941 tagtgaaaaa aataagcaag atagtcaaga taatggcgtg ttaaaaccag gaacagtgtg
     3001 gaaagaagaa gtaggcggac ttgttcataa tttaaaaatt atagatgata caacatggga
     3061 atattccgaa agtgtttggc atcctgaaca agtacaaata acagttgaga agctaaaaga
     3121 ctataaagga ttagaaagat ataaggtaat cgattcaggt ggagtagatt tattcatagg
     3181 taaaacagcc ttaatagttc cctataacaa agacggttta gaaacgttta aatttattcc
     3241 aacaaacgaa aaagaacatc gagataaaag tgaaatatta aaagaataca gtactaaatc
     3301 tggatacaag ttacaaaaga ctagcgagtg attgctagtc tttttattta tcctgaagga
     3361 ggaatcacag tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aa
//
LOCUS       DACEND010000170         3371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEND010000170 DACEND010000000
VERSION     DACEND010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.176"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            251..577
                     /locus_tag="IW746_002481"
     CDS             251..577
                     /locus_tag="IW746_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079360.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(694..1038)
                     /locus_tag="IW746_002482"
     CDS             complement(694..1038)
                     /locus_tag="IW746_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079361.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1114..2361)
                     /locus_tag="IW746_002483"
     CDS             complement(1114..2361)
                     /locus_tag="IW746_002483"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1079362.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2358..3164)
                     /gene="proB"
                     /locus_tag="IW746_002484"
     CDS             complement(2358..3164)
                     /gene="proB"
                     /locus_tag="IW746_002484"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1079363.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          664 c          669 g         1051 t
ORIGIN      
        1 tttaccaaga ggttgtgaga ctgtcgttta actctgaatc acaaggaaca atttcaaaaa
       61 acagtttctc atgtttgttg aatattgtga cttatgattg aggagttggc agaagaacac
      121 catttattcg gaaaaagggg atgtgacacg atttttgtca cattcccttt ttttctcaaa
      181 ataatgaaac tggaaaattt tcagtaggta agctaaaatt catttaagaa aaagaatggg
      241 gggataatag atgctggaag aatattttca ggaagtcggg ttgacactcg ttgaattacc
      301 cattgaattt tcagatgatc ggttgattta tcgtggctat atcaaagaat tgccaataat
      361 caatgcggaa ggtgaaacta gaaaagcgat gcagcaaaaa atagctggta tgtatcaggt
      421 gtatcgggaa cagctactgg ctgaatcagc ggaagaagaa aaagaaaaaa cgatgaactt
      481 gtccatggat gagctgcttc gttattatga tggcgaaaca tttgacggat tttcgcttct
      541 taacgaagag gaaacagatt taccaggcag ctggtaggcg aacgccgttt atatggaaaa
      601 agaggctgtg acaaaatctt tgtcacagcc tctaaaaagt ccggcagttt tttgccgatc
      661 ttcaaggcac tctacttatc gtacggatat tttttagcgg aaagtccgat tcaagtggtt
      721 tttattcagc tgatatttat aagataagtg aaaaatccca gcaatcagta gcgtaatcgt
      781 ataagcgatt gaatgatcga aaaaagaata gtaattgatt gtcgctaata agcataatgc
      841 aaccatgtaa ctaatgtgca agcgtgtaac cagccctttt tgcccgattt tgtcaaaata
      901 ctgcgaccat ttttcccgtt gactccctat gcttcgtttt ttgaattgaa agaaaaggga
      961 taaaataccg actcccagaa aagcagcgat attcagagaa ataaggtaaa taaaaaaaat
     1021 cattaaaatt tcaaacatat tgacaccttt ctagttggat agaaccttta cagaaaaaag
     1081 gcaaaaggcg ggaataatgt ttcacacgaa acattattcg cggatttgtc catcgccata
     1141 aatgatatat tttgtggaag ttaattcatt taaccccatt ggaccacggg catgcaactt
     1201 ttgagtagag ataccaatct ctgcaccaaa accaaatacc gatccgtctg taaaacgagt
     1261 ggaggcattg acatagacag ccgccgcgtc cacttcattt aagaaacgtt gactagccgc
     1321 ataactatct gtcacgatcg tttctgaatg tttagtatta tgccgattga tatgtgcaat
     1381 tgcttcatcc aaagaatcga ccactttgac agcaaggata taatctagat attcagtatc
     1441 ccaatctgtg tcttctgcta aagcagccgt ctctagatat tctaacgcac gagcatccgc
     1501 acgtaaagag accccgtgtt ctaccagttc tttttcaata gctggcaaga aaaaaggtgc
     1561 gatctcagaa tggatcagca atgtttctgc cgcgttacag acagatggac gggaagcttt
     1621 tgcattgacg atgatgttca ctgccataga taactgtgct tctttatcga tatatacgtg
     1681 attatttcct gtacctgttt caatgatagg taccgttgcc tgttctttga ctcgttggat
     1741 taattttgct ccgcctcgag gaatcagcac atctaagtat tctgtcaatc gcatcaactg
     1801 tcctgcagtt tcatgagaag tatcatcgac aaactggatg gcataaggag attcgccagc
     1861 actgaccagt acttgctgca ataattggac taataattga ttcgagaaga atgcttcttt
     1921 gccaccgcga aggataacgg cattccctga tttgaatgct agactagctg catctgtcgt
     1981 gacatttgga cgagattcgt aaatgatgcc ggtcacgcct agtggcacgc gctttttgcc
     2041 gattctcaat ccgtcggtgt tcgtccacat ctcatctact tttccaatag gatcgtctaa
     2101 cgaagcaatt ttttcgatct ctgtagccat ggcattgatt cgctcaggtg tcagctgcaa
     2161 gcggtcttgc atcgtttcac tgatattgtt ttcgactgct ttttctaagt ctttatgatt
     2221 tgcttcaatg atcgcttgtt gattcttttt gatggtttgt gccatttgaa caagtagctc
     2281 atttttgcgg tttgtaggca taagtgctag ttgttgcgcg ctatcttttg cctgttgtcc
     2341 taattgcatc aagtcagtca tttcaagtcc tccttaaata aagtacctac ttcttttcct
     2401 gcaagaatat caaaaataat tgccggattt tttccatttg ctaaaatcat tgcgctgttt
     2461 gcttcaaata tcctagaagc ggcttttaat ttactctgca taccgcctgt tccaaatgtg
     2521 ctaccagcac cacctgcttg tgccagaatc ttttcatcga ttttatcgat atagcgatac
     2581 atctgagcct cgggatttgt agtcggatta tctgaataaa acccgtcgat atcagaaagg
     2641 acgatcaaaa ggtctgccgc gacaagtttt gccatgatgg ctgacagctg atcattatcg
     2701 ccaaattttg tcagatgatc taattcatcc actgctacag tatcgttttc gttcacgata
     2761 gggatcgtgt ccataatcaa aagctgttcc aatgtattcc tcacattttt acgactttca
     2821 ggataatcta ccacgtctct tgtcacgagg atctgtgccg tctgttgatt atatacactg
     2881 aatctttgat tatagatatt catcagttcg gcttgaccaa cagccgccgc agcttgctgt
     2941 tcaggaatca aagtcggacg ttctgacaaa tgtaatttat gcataccaac gcctacagct
     3001 cctgatgaga ctaagatcac ttcctttccc cgatttctaa gatcagaaag gacaaaagct
     3061 aactgatcga ttgccgcaag attgatattt ccattgggat aaatcaatgt acttgttcct
     3121 actttgacaa taattcgttt tgcattttta atttctttac gcatgtgaca cctcaatcaa
     3181 atatccactc attaatttcg ttctattcta cttgttttga cgagtaaagt catgtaaaaa
     3241 atagtttttt tgtacaactt gcagtcgttt ggaaaaagaa cgaattaatg gtttacttta
     3301 agatagcaga atttcttgaa gaatttgtaa agaaagtctt ttacagcctc ggatatgtta
     3361 gaataggagt a
//
LOCUS       DACEND010000171         3332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEND010000171 DACEND010000000
VERSION     DACEND010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.177"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..210)
                     /locus_tag="IW746_002485"
     CDS             complement(<1..210)
                     /locus_tag="IW746_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1079364.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IW746_002486"
     CDS             complement(309..623)
                     /locus_tag="IW746_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079365.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="IW746_002487"
     CDS             complement(691..1185)
                     /locus_tag="IW746_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079366.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1178..1492)
                     /locus_tag="IW746_002488"
     CDS             complement(1178..1492)
                     /locus_tag="IW746_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079367.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1708..3099)
                     /locus_tag="IW746_002489"
     CDS             complement(1708..3099)
                     /locus_tag="IW746_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1079368.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHMAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..>3332)
                     /locus_tag="IW746_002490"
     CDS             complement(3084..>3332)
                     /locus_tag="IW746_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079369.1"
                     /translation="NSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEYWIEKQ
                     TRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
BASE COUNT          984 a          620 c          448 g         1280 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattt gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ttcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 gccatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccatatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta tt
//
LOCUS       DACEND010000172         3330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEND010000172 DACEND010000000
VERSION     DACEND010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.178"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2232)
                     /locus_tag="IW746_002491"
     CDS             complement(<1..2232)
                     /locus_tag="IW746_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079370.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IW746_002492"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IW746_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          914 a          745 c          537 g         1134 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata
//
LOCUS       DACEND010000173         3313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEND010000173 DACEND010000000
VERSION     DACEND010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.179"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..185
                     /locus_tag="IW746_002493"
     CDS             <1..185
                     /locus_tag="IW746_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079371.1"
                     /translation="MGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQ
                     KYDLETGGNDNEYFND"
     gene            166..882
                     /locus_tag="IW746_002494"
     CDS             166..882
                     /locus_tag="IW746_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1079372.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            882..2330
                     /locus_tag="IW746_002495"
     CDS             882..2330
                     /locus_tag="IW746_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1079373.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2400..2909)
                     /locus_tag="IW746_002496"
     CDS             complement(2400..2909)
                     /locus_tag="IW746_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079374.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2899..>3313)
                     /locus_tag="IW746_002497"
     CDS             complement(2899..>3313)
                     /locus_tag="IW746_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079375.1"
                     /translation="LINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVY
                     EALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFA
                     QTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1158 a          565 c          683 g          907 t
ORIGIN      
        1 ttatgggctt actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt
       61 attttctgat tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag
      121 aaaagaaatt acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg
      181 attgaagata atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg
      241 gaagcaactt tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa
      301 cattgggata tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga
      361 agagagatcc gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct
      421 gaaagcgatc aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc
      481 agtcctctga cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa
      541 gcaagagaag aagcagtagt ttcagatgat cattttgata tcgagactag tcatttcaag
      601 atgaatacga aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct
      661 aaagagttcg atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag
      721 cagttgcttg aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc
      781 catatcaaga aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt
      841 tggggagtcg gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc
      901 aactcttagc tttctgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta
      961 cacaactgac aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg
     1021 aatcagttga aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc
     1081 aggcgtttca aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact
     1141 ttatatttat cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt
     1201 ttaccttaac agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat
     1261 cgaataaaaa tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt
     1321 tgaatcatga attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca
     1381 gcgttcgtcc aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag
     1441 ttattgccag ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg
     1501 cgacgaaaga agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg
     1561 aatttgatga gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag
     1621 cagaaattga agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga
     1681 tgagaacacc tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc
     1741 ctgaaaatca gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc
     1801 gtttggtgaa tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca
     1861 ttaaaaaatt caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag
     1921 cagaagcagc aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg
     1981 attatgaccg atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg
     2041 aaaacggcca aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg
     2101 aagatacggg aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata
     2161 aagtagatcc ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg
     2221 tgcagcaact tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag
     2281 gaacaaaatt cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg
     2341 gtataaaaga tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt
     2401 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     2461 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     2521 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     2581 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     2641 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     2701 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     2761 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     2821 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     2881 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     2941 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     3001 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     3061 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
     3121 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
     3181 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
     3241 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg
     3301 ccaattgatc aat
//
LOCUS       DACEND010000174         3310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEND010000174 DACEND010000000
VERSION     DACEND010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.180"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            124..1095
                     /locus_tag="IW746_002498"
     CDS             124..1095
                     /locus_tag="IW746_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1079376.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1127..2524
                     /locus_tag="IW746_002499"
     CDS             1127..2524
                     /locus_tag="IW746_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1079377.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2683..3306
                     /locus_tag="IW746_002500"
     CDS             2683..3306
                     /locus_tag="IW746_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079378.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1191 a          529 c          717 g          873 t
ORIGIN      
        1 tgctctactg aagactttag ttatatggat attgatgaca agcaacacta tgcaaagaat
       61 gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca ggagtgagaa
      121 tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg aacagtttcc
      181 cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc agaagcgatt
      241 tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa tcgaagcttc
      301 tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt tgtctcaggg
      361 atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt tgccgatcag
      421 ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga tgggctgatt
      481 gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat gagtattcca
      541 gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt agtgaatgat
      601 cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa aaaaatcgga
      661 tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg agccaaagaa
      721 gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta ttctagaaaa
      781 agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat ttttgtcagc
      841 aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt cagaatcggg
      901 aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag tatccttccg
      961 aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga acagctgtta
     1021 aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga aaatatcata
     1081 catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga aaaaatatca
     1141 atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag aaggaaggat
     1201 ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag aaccgcaaaa
     1261 gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt atcaagagga
     1321 tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc aatggagccg
     1381 tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct atcatcgtta
     1441 ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc atttcgatat
     1501 gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg aagcctatga
     1561 aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat ggtttactca
     1621 taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt atccagaaga
     1681 gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt cagcgttagc
     1741 catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct tgaatctgac
     1801 accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc aaatagcaga
     1861 tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc caagtgaatt
     1921 gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg atttgacggt
     1981 tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac gccgggtaaa
     2041 agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt atttcgacaa
     2101 ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct atgaacaagg
     2161 aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact gctttatttc
     2221 tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg gcatgatcca
     2281 cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc aagccattca
     2341 agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact ggtcgtggac
     2401 caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag atggaaaaag
     2461 aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg gatttgaggc
     2521 ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca ttttgtgaaa
     2581 aagaagttca attatttacg atctttttca acttttttgt ctttctattt cattttttgt
     2641 atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa aaaaagaccg
     2701 tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc agcattttca
     2761 ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc aagcaaagaa
     2821 tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta taaaataact
     2881 gccaattacg aaaaaggcga attggacatt tatttgccaa aaggaaaaga agaaaatcaa
     2941 tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca atttaaagac
     3001 aagcatttga ccgttttgct tttgactcat gatggcgaca aagtagcccg tttgaaaaat
     3061 ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc cgatcgttta
     3121 gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc tcaaaaaaac
     3181 gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac gggaagtgaa
     3241 gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca atcctatgga
     3301 caatgaaaaa
//
LOCUS       DACEND010000175         3289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEND010000175 DACEND010000000
VERSION     DACEND010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.182"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..847
                     /locus_tag="IW746_002501"
     CDS             <1..847
                     /locus_tag="IW746_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1079379.1"
                     /translation="DKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVE
                     ATKKAREFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILL
                     VLDGSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEI
                     YSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFT
                     KKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYM
                     WQMKY"
     gene            829..1290
                     /locus_tag="IW746_002502"
     CDS             829..1290
                     /locus_tag="IW746_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1079380.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1340..1804
                     /locus_tag="IW746_002503"
     CDS             1340..1804
                     /locus_tag="IW746_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1079381.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1818..2567
                     /locus_tag="IW746_002504"
     CDS             1818..2567
                     /locus_tag="IW746_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1079382.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2567..>3289
                     /locus_tag="IW746_002505"
     CDS             2567..>3289
                     /locus_tag="IW746_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1079383.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLV"
BASE COUNT         1092 a          452 c          635 g         1110 t
ORIGIN      
        1 tgataaagaa ttaactgtac cttgtcatgt ttatactgct aacgaattta actatgctat
       61 accaaaagga ttatctgaaa attctttagt tatcacttgt tcccattctg gcacaacacc
      121 agaaactgta gaagcaacaa aaaaagcaag agaatttgga gcaatgacta tttgtttatc
      181 taatgaagaa ggatctcctc tatgggaagc agctgaatat cctgttcact atgattgggg
      241 taaagatgtt gatgctagtg atttaaataa aggaattctt tatggtttat tatttaatat
      301 tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc ttgaatgaat ttgataagtt
      361 aaatgatctt gttattgctg aaaagagcaa atttgaaata actgctaaag aatggggtaa
      421 aaaccaaaaa cgagataaaa ttatttacac aattggtagc ggtattaatt acggagaaat
      481 ttactccaca gcagcttgct ggttcatgga aatgcaatgg attaattcta atgctattca
      541 ttctggggaa tttttccatg gtccttttga aatcactgat tatgatgtac cattcatctt
      601 agtgaaaagt attggagata caagattttt agatcaacgg gtagaagact tcactaaaaa
      661 attcagtaat aaattaatgg ttttagacca agctgatttt gacttagaat ctatagatag
      721 tgtagcgaaa gaatatattg cagctgtact tagtggcgtt gttattcgcc aaatggtaga
      781 agcgattgct tttgaaagag gccattcctt agatgttaga cgctatatgt ggcaaatgaa
      841 atattaatta ataggaggtt gcaaaagatg gcaaatcaat tatttcacat tgtgattaca
      901 acacattcta ccctttgtga gggttattta aaggcaacaa aattaatttt aatgaatgac
      961 gccacaggta ttagtgctgt tccatttaaa gaaaatacta gtccagatga ttttgaggag
     1021 agtatgaaag aaataattag tcagcatcat ttgcaacctc taattatttt aacggattta
     1081 attggaggaa cacctaataa tgtttctatt aaacagttga acaacaatag aattcaaatt
     1141 attgctggga ttaatttacc tcttttactg gagttattaa tggcccagtc agctggtaaa
     1201 tctatagaag aaatagattt taaggaatta ctgaataact ctcaaaaaag tatggttcat
     1261 attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt tctattctat aaattttaaa
     1321 aattgaagga ggaaatagaa tgattaaatt agtgcgtgta gaccacagat tacttcatgg
     1381 acaagtaata ttttcttgga ctaaacagta tgatattaac catattattg tggctgataa
     1441 caaagttcca ggagatccaa tgtctgtgat ggctttgaca attgctaaac ctgctgattg
     1501 caaattagac attgttaaaa ttagtgatgt taaagggatt atggaaacat ccccaaatga
     1561 taattacatg attttaatta aaggacctaa ggaagctttt gaattagttc aaaatttacc
     1621 agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa agcgagtcca aacaatatgg
     1681 aaaggcagtc tttttaactc ctgaagaatt agaagccaca aaaaaaattc ttgatcaaga
     1741 agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt gaaaatgtag attttagtaa
     1801 gtaagggggg aaaataaatg ttattaaaag ccattttatt aggtttagtt ggtgtggtag
     1861 ctgttattga ctccaggtta attggtcgac aaaatattgg tagaccacta attttgagta
     1921 cacttgccgg acttgtgcta ggtgacttgc atacagggat tgttcttggg gcatctttag
     1981 aattaatttc tatgggattc gtctcaattg gggcggctgg acctcctaat atgcaacttg
     2041 gtagtattat tgccactgct tttgcaattt taactaatag tagtacagaa acagccttaa
     2101 caatagctat cccagtggct gtagctggtg aatttatgag tattatcatg cgtatgataa
     2161 ttgctcaatt tggacatgct gcagataaag ccattgaaaa taataaattt agaaaagctc
     2221 aaattattca tatttattgg tcatttattt ttaatgcttt tgtttacttt attccaatct
     2281 ttttaacagt atattttggt gctgacgttg tggcagattt agtagataaa attccagaaa
     2341 tgattacaga cgccttgact gtctctggaa atatgctttc cgcattaggg tttgcaatgc
     2401 tattaagcac aatgttgagt aaaaaattat atccttactt tatttttgga ttctttattg
     2461 ttgcctattc aggtctttca ctaattggcg tgacaatttt tgcagctctg attgcctttg
     2521 taatggatca agttaaatat ggaaaaagag aagaggcgca tggttaatgg aaaatagtac
     2581 agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag atgtgtcttc gttcatggac
     2641 aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg ggatttatgt atgggatggc
     2701 gccaatttta aataaagttt ataaaaatga tgaagagaaa aaaagagaag cttatactcg
     2761 tcatatggca ttttttaact gtacgcctca aatgactcca tttattatgg gactttgtgc
     2821 ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac tttaatactg aatctatctc
     2881 catgattaaa accagtttaa tgggtccttt tgctggaatt ggtgattcat ttttccaagg
     2941 tactttaaga attattacat ttgggattgg attgtctttt gctaagcaag gtagtatttt
     3001 aggaccgatt ttagctgttg gcctttttgc tataccttct attttagctg ttttttacgg
     3061 cggtaaatta ggatatgagt ctggcaataa ataccttact aaattgtatc aagaaggtct
     3121 tatggatcga gtaatgcaat ttgcttctat cattggtttg gctgtagtag gtggaatggt
     3181 ggctagcatg gtgccaattg tcaccccttt aaaattcact acagggggaa cagaacttgt
     3241 tttacaagac ttacttgatt ccattattcc tggattatta ccacttgta
//
LOCUS       DACEND010000176         3285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEND010000176 DACEND010000000
VERSION     DACEND010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.183"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..1398
                     /locus_tag="IW746_002506"
     CDS             7..1398
                     /locus_tag="IW746_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1079384.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IW746_002507"
     CDS             1414..2454
                     /locus_tag="IW746_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1079385.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..>3285
                     /locus_tag="IW746_002508"
     CDS             2473..>3285
                     /locus_tag="IW746_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1079386.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMD"
BASE COUNT         1160 a          484 c          616 g         1025 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggat
//
LOCUS       DACEND010000177         3277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEND010000177 DACEND010000000
VERSION     DACEND010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.184"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..180
                     /locus_tag="IW746_002509"
     CDS             <1..180
                     /locus_tag="IW746_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1079387.1"
                     /translation="ENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQIL
                     NDGEDIKWIQKQIAY"
     gene            153..830
                     /locus_tag="IW746_002510"
     CDS             153..830
                     /locus_tag="IW746_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1079388.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            820..1962
                     /locus_tag="IW746_002511"
     CDS             820..1962
                     /locus_tag="IW746_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1079389.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1975..2952
                     /locus_tag="IW746_002512"
     CDS             1975..2952
                     /locus_tag="IW746_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1079390.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            2952..>3277
                     /locus_tag="IW746_002513"
     CDS             2952..>3277
                     /locus_tag="IW746_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1079391.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKN"
BASE COUNT         1220 a          403 c          626 g         1028 t
ORIGIN      
        1 gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg
       61 aaaaatgtta agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg
      121 aatcaaatat tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa
      181 aatatactag tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg
      241 aaaccttaag attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa
      301 tgcaagatct gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa
      361 aaaaaatcta taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa
      421 aaaaattgaa tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg
      481 caaaagaaat ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag
      541 atgctgtaat tggagagcat gctataataa acacaggtag cattgttgag catgattgta
      601 gaattaataa ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag
      661 gtgtaggaac tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt
      721 ggtgtatgat aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt
      781 atggcaatcc agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta
      841 tcaagtcctc atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat
      901 tggatagcgc cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact
      961 ggttctaagg gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta
     1021 agactgctta atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct
     1081 acagtaaatc ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat
     1141 acatggaata tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac
     1201 caattaccta aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga
     1261 attttagcta ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta
     1321 ggttctactt ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt
     1381 aacggtaata agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa
     1441 tatgttgagc attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa
     1501 catagcgaaa taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga
     1561 caaatggaag tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag
     1621 gaagcttttt ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca
     1681 aatcgttggt tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac
     1741 gtaataaata aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat
     1801 ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca
     1861 gaaaagttat ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa
     1921 caagaatttg ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac
     1981 ataggaatta ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac
     2041 tttaatgata ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa
     2101 aaggaatttg gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata
     2161 aattcagttt ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta
     2221 aatttagatt gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata
     2281 atcgaagtag ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga
     2341 aaagaaattc aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac
     2401 catgtaggac agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt
     2461 tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata
     2521 gatacatttg ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat
     2581 attgattatt cggacagttt cactcttatt attgagcatg aaactggtat agttggaact
     2641 ttaagtgtag atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag
     2701 attttaatag aatgggaagg taacccggat tctctatttt catggaatga agaaacttct
     2761 agtatgacag atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact
     2821 attattgaag atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa
     2881 atggaacccc aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt
     2941 gaggtaaatt aatgatgaaa gtaatttttt ttgggttagg gtcaattgga aagagacatc
     3001 taaaaaatct aataagctat ggaaaaaaga attctattaa ttttgatata acagcttata
     3061 gtagcagtgg aaaaaaagag tttgaagaaa taacgtatat tcaaaataga gacgatttgg
     3121 atacacatta tgatatagct tttattacaa atccgacttt taagcatctg gaaacactaa
     3181 atgtaattgt tgaaaaagtt gattacatat ttttagaaaa acctgctttt tctgagagta
     3241 ttgatcttac tcaatataat tatgattaca aaaatat
//
LOCUS       DACEND010000178         3240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEND010000178 DACEND010000000
VERSION     DACEND010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.185"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            447..2465
                     /locus_tag="IW746_002514"
     CDS             447..2465
                     /locus_tag="IW746_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1079392.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2525..3217)
                     /locus_tag="IW746_002515"
     CDS             complement(2525..3217)
                     /locus_tag="IW746_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1079393.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         1002 a          593 c          572 g         1073 t
ORIGIN      
        1 atggacagag tattttatgg attttttatt tatttttaat gggttatggt ttgaaagaat
       61 ggaactggaa agaaaaggtc cgctataagc ttcctcaagt atttatcagc ttgattgttt
      121 tattcggttt gattattttg atgacacaaa tttcactcat catcagagga aatacagata
      181 cagcgctgag gttttctaaa ccatattctc tcttcgcaat gtattatact ttctcttcgt
      241 ttttattttt agaaatgcta tctgataaat taggtataaa aggaagagga aattttcttg
      301 ctgtattttt gattgtcact caagttatat gtaatttccc tttagtttct tatcgcgtat
      361 ctacatttat gaaacgtgaa tttccaaact cagggaaggc atggctgata aatatcgggc
      421 agttttttat ttgctattta ttagcagtgt ccctcctagt tatcttttta ttttatatac
      481 aaaaaatccg tttcattcat cgtttcatcg aatacttcgc ttttgattcg attgaacaat
      541 taactcagcg ttggaaaaaa agaattcatt ggctttttgt tcataaaagt tattttttag
      601 ttgcagcatt tttttactgc tttaccttcg tgcagatttt cgttttagaa ccaaaagaag
      661 gttggaaaga aactatccag gttgccttaa aaatatttac tcagcgacaa gcacccatga
      721 tcctgacaat catcattatt atgggatttt tcttcttact attgttgatt tccaatcgtt
      781 tttggtacgc cttgactgct accttgatca ttaacttact gctcactatt tcgacagtaa
      841 tcaaaatgga aatgcgggaa gagcctgtct ttccttcaga tttaaaaatg ctgaccggat
      901 tgtcagaact attatcgatg gttagcccag tcttattaat cgtgggcgga ttgatccttc
      961 tcctattatt gattactagt attattgtac aacgtagact acaaaaacag tattccttga
     1021 agattcattg gaaacgtcga tttatcagta tcgctgtatt attaggatgt ttttcgggtg
     1081 tctttttcat taatcacaaa aattcgcctt ctttcttgct gtttaattta tttaaagtaa
     1141 acaagacttt ctttaaccag caaggcgccg taaaagataa cggaccagtc attcaattcc
     1201 taaacaatat cgacattaaa atcatggaag aacctgctgg ctattcgaaa gaaaaaatta
     1261 atggaataat gaaaaagtac aacacaaagg cagatgaaat caaccaaaca agaagtgact
     1321 ggttaaacaa tcagacgatc gttctcaatt taagtgaaag cttcagtgat cctgcacgtg
     1381 tccctaatct gacagtacct acagacccta ttccttacat caaaagcatt ataacagaag
     1441 gaacaggagg attgatgtta tccgtcggtt atggtggcgg aacagctaac atggaatggg
     1501 aaggactgac aggcttgagt atcagtaacc tgtctgcttc gttagttaca ccttataccc
     1561 agctagtgga gcgtcagaaa ataagtccaa atattaccga tctattcgat gaaaaaatag
     1621 ccatccatcc ttttacagct gctctttatc gcagaaaaca agtgtttgat aaatttggtt
     1681 ttgacaggtt ctattattta gacagtccgg ataaattaac ttatacggat aaaatagaac
     1741 aaagcccacg catatcggat gattcagcat acaaagaaac cttgaaagct ttacaaagca
     1801 atacagaaac gtcacaattc atccaattat ccactatgca aaaccatatg ccttatgatg
     1861 attactatag tgaattgaac tacactgctg aaggaactgc tgtcattcct aatagaagaa
     1921 aagaattaca aacttatatg caaggccttc actataccga tacagcagta aaaaaattca
     1981 tccaagaaat cgacaaaatc gataaaccga ttacctttgt tttttatggt gaccatttac
     2041 ccgcaatcta tagtgggaat gattcgaaaa aatatggatt agaacagcac cagacagatt
     2101 atttcattta taacaacaag tatagtcgtg aacaagcagc tacgttaaat aaagaagttg
     2161 ttgctcctaa taactttggt gctatggctc ttgagcatgc gaatatcaaa gtaactcctt
     2221 actatgcgtt gttgacagaa gtatctaatc atctacctgc attaacaatt gatcctacag
     2281 aaagcttgtc taatcgtttt aatggaaaac aggtctttgt tacagaggat agtcaaatta
     2341 tccaagaaaa aaatttaact gagaaacaaa aagaaattct agaagattat cgtctgatac
     2401 aatatgatct tgttgcaggt aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag
     2461 actaacaaat aaactgagca aggggttgtg ataaaagaca tgtcacaacc ccttgtttat
     2521 ctcattaatt tgcttgtcca taaacgattt gattaaagcc atttttcaat tcattattcg
     2581 ctgtctcgtt gaagtaaaga taattatcgg gattcccctg attttgttgt gtcaccaata
     2641 cttttggctg attttgctga aacccgaata gatagacgtg ctttttcaga tatggttctg
     2701 tctctgcatc ggaatagacg gctgtgattt gatagtctgc ttgacctgtc ccactttctg
     2761 accattctgc agaaacaggc actccaccaa tagccatctt ccactccccg ttcaaaatag
     2821 cttgagggac tttaagacca tataagtcca cactcatttg attcgtataa cttttgtatt
     2881 gttgtcccat tgtttggccc catttggtca taaatgcctc taattgtgcg gatttgtctg
     2941 cattccacag tgtttgttca acttgttcgc tgctttctga agaggaagat gaagcctcta
     3001 cagaagaact tgaagtagat gtttgagttg tttcagtcgt tttggaagac tctgacgaag
     3061 tagaggtatc tgctgtttct gtacttgctg cagttgactt gctctctgtg ctagttgttg
     3121 tagtagttgc aactgttgct ttcgctgttt cttttgttga ttcttttttc gattcattcc
     3181 cgttggtaca accagctaac aaaaaagcta gaccaattgt tactaaaatc ctttttttca
//
LOCUS       DACEND010000179         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEND010000179 DACEND010000000
VERSION     DACEND010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.186"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW746_002516"
     CDS             complement(543..2048)
                     /locus_tag="IW746_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1079394.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW746_002517"
     CDS             complement(2045..2731)
                     /locus_tag="IW746_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1079395.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..>3221)
                     /locus_tag="IW746_002518"
     CDS             complement(2924..>3221)
                     /locus_tag="IW746_002518"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1079396.1"
                     /translation="KITDAYDKNPEIENLLLDDYFVEITKKYQQSVRDVVALAVQAGV
                     PVPTFSSAIAYFDSYRAERLPANIIQAQRDYFGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT          923 a          640 c          513 g         1145 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt t
//
LOCUS       DACEND010000180         3216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEND010000180 DACEND010000000
VERSION     DACEND010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.187"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW746_002519"
     CDS             204..1172
                     /locus_tag="IW746_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1079397.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW746_002520"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW746_002520"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1079398.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW746_002521"
     CDS             2202..2810
                     /locus_tag="IW746_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1079399.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          956 a          615 c          778 g          867 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
     3181 ggaagattct agaaatattt catacttcca actata
//
LOCUS       DACEND010000181         3187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEND010000181 DACEND010000000
VERSION     DACEND010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.188"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(407..1021)
                     /locus_tag="IW746_002522"
     CDS             complement(407..1021)
                     /locus_tag="IW746_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1079400.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1627..2004)
                     /locus_tag="IW746_002523"
     CDS             complement(1627..2004)
                     /locus_tag="IW746_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079401.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2304..3185)
                     /gene="galU"
                     /locus_tag="IW746_002524"
     CDS             complement(2304..3185)
                     /gene="galU"
                     /locus_tag="IW746_002524"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1079402.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          968 a          658 c          493 g         1068 t
ORIGIN      
        1 tcctgttgga caattttgtt atatcaggta aacttcattg caataatgat aacaaatgta
       61 ctataattac tttgtaaaga gttttgggga agactctttc atcaacgtgt attaagagac
      121 aagaaagaat ccatatggat tctttcttgt ctctttttcc actacttttt atttttctga
      181 cactacatat tcttataact catttctcat aactgtgaaa caacttataa aaataacaaa
      241 ctttaaacaa aacaagctta aaatcaaaga tgatccatct taaaataact aaaacaaaaa
      301 aagtagtcgt tgttaatcct cattaataat cgttttcttt ttttcaaacg ctccattttt
      361 atgtggaaca ttattttttt aaggaacttt gtcaacatgt ggataactat tttccacttg
      421 tgtcctggac tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg
      481 caagagaggc tgttccgcag atcgctggat ctagatttgg caaagtcaag actgcatcat
      541 gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc aaaccactgc
      601 tatgttgcga tttgatactt cgtgacaaat caaatccagt aacaatgatt aatggatgac
      661 cattttcttt gatccactga gctagttcga tgattgccgg gtttcttcct tcattcgaaa
      721 gaagaagaaa aatgtcttct gatttgacag aaagctgatc aataattgcc tgtcctgcac
      781 cttctagcgt ttcatagaca cctccggctg gattttcaat caatatactt ggtacaagat
      841 ccggggtttg atcccaaaat tcaaatgcta caccacttag tgtgcggcta gcatatatct
      901 gcaatctacc accttgatta atttgtttcg cgatccaagt gccagcttga agcatcagcg
      961 gctcttctat ccttaatttc tcttccaatt ggtgataata gtcaaaataa cataaactca
     1021 tttatctctc ccgcctagct atacttgaca tgaatagaaa cagctgccta atctaaggaa
     1081 gtacataaaa aaacagtagt tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa
     1141 aaaaggcatt gaacgaatac gcccaatacc ttgacatagc aaataaaaca acgtgcagac
     1201 catggaaaat atctacatag gggtagaatt ttcaccctag attaggtaca ataaattagg
     1261 tcgatgcact gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc
     1321 attccctgat tcttagcgcc aactaagggt cagggagcct ttttttattc agctgtttct
     1381 atctgaattt attgtaagta atctcttatc caagtgcaag caagaaaata caaaaaaaca
     1441 ttggtatgat ttatatatat ataaataaca taaaaaaagg aatggcttat cccgatacaa
     1501 caagcattcc tttaaaaaag attcttttat ataagtgaaa aaagcttttt tccaccaatt
     1561 aaataaatct ctcatctcaa tagtttatcc attattttat ctttttttgc gtatacctac
     1621 taaatattac cgtcttcttc tcgttcttct actttgttcg agagaatctg tactagaaaa
     1681 agtagttgta tgtctacctt ttctgcctat ccgagacttt cttttcatat atgggatata
     1741 aatgattaga tacagtacag caaaaaataa agcaaatacg caagcttccc aactacctaa
     1801 cgccaattca accatcaagg tagcaacccc aacggatgct gcataccaga taaaaatcag
     1861 aaacgtttct ttttttctga ccatgcgaaa catgataaat agactaataa gcaatagtgg
     1921 caacacaaat atccaattgc ttccttccat cagccacttg agtatatcaa gcagcgactg
     1981 tgcaacagcg actaattttc ccatagtgaa cacacacctt tttttataat taaaaaattc
     2041 gcctttttat tctactatga atcttctggc ttggctttca tttctcttat gtaatcattt
     2101 tcttttatac aacttaaaaa aaaattaaac cattagcata atattttctg aatctattca
     2161 cgaaagtctt tttctatctt tcgttttcca cgactcaact gatctagagt caaaaaacta
     2221 aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta ataacaaata gcctttcatt
     2281 ctcagcaggc cacttatttc cagttaactt tctgttctta gctttttact caactggata
     2341 atatatgcac ttaatgaagt cccaatttcc gaatgttgaa gtccatattc aatatttgtc
     2401 ttcatatatc ctaacttatc gcctacgtcg tatctttttc ctttaaactc tctcgcatag
     2461 accgcctctg tctggttaag agtctgaagt gcatccgtta gctgtatctc gtttcctgct
     2521 cctggttctt gagtttctaa tatctcaaat attttaggag tcagtagata acgtccgata
     2581 atggctaaat tacttggtgc ttttcccatt tcaggttttt ctacaaaccc ttttactctg
     2641 caacgaccct tttcgctatc tgttttagga tcaataattc cgtatttaga tgtttcattt
     2701 tctggtacac gcatgacggc aacagcggaa gcttgtgttt cttcataatc catcatcaat
     2761 tgttgagtca atgggatatc atctttcatc aaatcatccc ccagcatgac gacaaacggt
     2821 tcatttccta taaatgcttt tgcttgaagc acggcatccc ccagtcctct tggatgagat
     2881 tgacggatga agtgaagatt gacttgagtc gtttcttcaa ctaaagcgag taattctgtt
     2941 ttttcctttt cccgcaaatt gttttccaat tcgatatttg catcaaagtg atcttcaatg
     3001 ggtctttttt cttttcccgt tacgatcaag atgtcttcga ttcccgaatc caatgcttct
     3061 tccacaataa actggatcgt tggtttatcc acgattggaa gcatttcttt tgccatggct
     3121 tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag gaatgactgc ttttttgacc
     3181 ttcactt
//
LOCUS       DACEND010000182         3182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEND010000182 DACEND010000000
VERSION     DACEND010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.189"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW746_002525"
     CDS             179..775
                     /locus_tag="IW746_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1079403.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW746_002526"
     CDS             899..2698
                     /locus_tag="IW746_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1079404.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..>3182)
                     /locus_tag="IW746_002527"
     CDS             complement(2976..>3182)
                     /locus_tag="IW746_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079405.1"
                     /translation="KAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRDYQ
                     LINESKYRMLVKFCDISAPAELVS"
BASE COUNT          901 a          564 c          590 g         1127 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tt
//
LOCUS       DACEND010000183         3133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEND010000183 DACEND010000000
VERSION     DACEND010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.190"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..1041
                     /locus_tag="IW746_002528"
     CDS             208..1041
                     /locus_tag="IW746_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1079406.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="IW746_002529"
     CDS             complement(1143..2843)
                     /locus_tag="IW746_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079407.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1110 a          520 c          623 g          880 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DACEND010000184         2968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEND010000184 DACEND010000000
VERSION     DACEND010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.192"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..1031)
                     /locus_tag="IW746_002530"
     CDS             complement(84..1031)
                     /locus_tag="IW746_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1079408.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IW746_002531"
     CDS             1277..1741
                     /locus_tag="IW746_002531"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1079409.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IW746_002532"
     CDS             complement(1803..2846)
                     /locus_tag="IW746_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1079410.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          981 a          587 c          567 g          833 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaa
//
LOCUS       DACEND010000185         2946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEND010000185 DACEND010000000
VERSION     DACEND010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.193"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..899)
                     /locus_tag="IW746_002533"
     CDS             complement(<1..899)
                     /locus_tag="IW746_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1079411.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKAD"
     gene            complement(1043..1774)
                     /locus_tag="IW746_002534"
     CDS             complement(1043..1774)
                     /locus_tag="IW746_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1079412.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(1843..2784)
                     /locus_tag="IW746_002535"
     CDS             complement(1843..2784)
                     /locus_tag="IW746_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1079413.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT          791 a          661 c          500 g          994 t
ORIGIN      
        1 tcatctgcct tttcagtcaa ttcattgcgc agttctcgct cgattgctgg ctgaataaag
       61 cgtttataac tatcctgata ggcttctctg acgaatgaag aagaaggaga ggcaggattt
      121 ttgactattt ggcgatccaa gtaagctaaa attgctgctt catctacttg aaagaatact
      181 ttcagaatat cttctttttc tccacgatta gtagctaata ttcgatgaga aaccatttta
      241 tggacgggtt ctgcaaagtc ataatacatt tcatatacgc ctttttcatc tgcttgctca
      301 tccttgacat tgctgacata catacctttg ttatacgtat aagaccgaat ccaagtccga
      361 aattttgcat tatcgctgac ttgttcagca ataatctcat gagcaccgtg caacgcttct
      421 tcggcagaag agacttcttt ttctttgtca atatattttt ctgcttcaga ttgcacctct
      481 ccatctgtaa gctgcatcag ccacttcgcg agtggttcaa gtcctttttc tttagcaatc
      541 gttgcttttg tccgtcgttt ttgtttgtat ggacgataca aatcttccac ttgttgcatt
      601 ttcacggctt gggtgatttc tgcttctaac tcgggagtca gtttcccttg ttcatcgatt
      661 agccgaatca cttcagtttt acgtttttct aagttttcaa gataggcata ccgctcttcg
      721 atttcacgaa tctggacttc atcaagactt ccagtcattt cttttcggta acgcgcgata
      781 aaaggaacag tgtttccttc atttaacaaa ttaagtacag tagtcaactg cttaggacga
      841 tagtctgaaa gttctttttg tacaagctga atgatcgtct ggttcaaatt ttccgtcatt
      901 aaaatgcctc ttttctgcta aaaattgctt ctttattcta tcatactttt tatcctgggt
      961 taaactactg aaagaaacaa tattctctat aaaaataaaa agagccgcaa caaagtcaca
     1021 gctccttcat tctttccatt agtcagattc catgccaagg tccttcgttg ttctgatatt
     1081 caatatcttg aagaatacct tccttgtcaa aaacaacccc atgcaatgac cctccgaata
     1141 cagccccacc gtccattccg attttatgat cctgaatcca gaggtctgtt gtctgcatat
     1201 ctccatggag cattggtgta atcgtatgtc caaatacaat tgtcttacct gtattgtttt
     1261 tcccttcatg aaatggttcc cttatccaaa taaagtctct tagacttgtc ttacgccagt
     1321 ctttctttgt caaatccaca ccggcatgga caaaaagata attttcccat tcataataca
     1381 aggggcgctg gactaaaaat ttaatcagtt ctgaatatct cgattggatc atcatcgaga
     1441 tttcagttgg ggagtattcc tccgatgccc ctgggtgaat caggctttca aacgtttctt
     1501 ttccaccatt tctcatataa ggcgcatacc agtcctccgg tgattgcaga aattgcaaaa
     1561 aatattcttc atggtttccc cggaggtaaa tggctcctgt ttcttcgact aattgctttc
     1621 ctaataaaaa acattctttt gtacgaggac ctcgatcatt gagatcccct aataaaacaa
     1681 gctgatggat ctttggatca taatagtgaa taagtttttc aaaaagctca tgtttgccat
     1741 ggacatcccc aatagcataa atcaatgatt tcatgtggat caccatcctc aacgctagta
     1801 tagcgcatat tggaaatggt cgacaatcaa aactattgtt ttctatttaa ttaaagctgt
     1861 aaaccgcagt tctgcttctt ctggagagat tccatggtct tgaacatagc gattgcgcgg
     1921 atgtttcgca cactctatgc tacatgctcc taaatgtttt tcttcatttt caatggaagc
     1981 aagaatctga cgattgcact caggattgct acaattgata tatcgttcac atggtgttcc
     2041 gtcaaaccag tcgcgaccga cgatttgttt atctacttgg ttgatatcga cagcgatccg
     2101 ttcatcaaac acatacatct tgccgtccca taattctcct tggacttcag gatccttacc
     2161 ataagtagca ataccgccat gcagctgtcc gacatctttg aatccttcac ggacaagcca
     2221 accagaaaac ttctcgcaac gaatgccacc agtacagtag gtcactaccc gtttttccat
     2281 aaattcttct ttgtgctcgc ggatccagtc tggaagctct cgaaaactgc gtatatctgg
     2341 gcgtacagcc cctcgaaaat gtcccaagtc atattcataa tcattccgtg catcaatcac
     2401 cactgtctgt tcatctaaaa tagcttcttt aaattcttgc ggtgaaagat aggcacctgt
     2461 cacttggttt ggatcaatgt cctcttctag ctttaaactt acaagctctg gacggtatct
     2521 tacaaacatc ttcttaaagg catgtccttc gcttggatcg atcttgaaca tcgtatcagc
     2581 aaatcgagga tcagctttca ttgctttcat ataggcagtc gtgttttcaa cggttccaga
     2641 cacagtacca tttattcctt cttcagctac tagaatacga cctttaagat ccaaagattt
     2701 gcaaaaggct aaatgatttt tggcaaattc ctctggttct tcgatttttg tataattgta
     2761 ataaagcagt acttgataat ccatgtattt cttccttcct tcttttcgtt cctaaacagt
     2821 ataaagaagg cgttcgcatt tgacgattta tttgcttgtg tataattacg catggaaagt
     2881 tttttgttta cataatatta ttatttgaat taaaaagact tcgctttcct gtttcgcaca
     2941 gaaaaa
//
LOCUS       DACEND010000186         2904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEND010000186 DACEND010000000
VERSION     DACEND010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.194"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            239..928
                     /locus_tag="IW746_002536"
     CDS             239..928
                     /locus_tag="IW746_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1079414.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            942..2444
                     /locus_tag="IW746_002537"
     CDS             942..2444
                     /locus_tag="IW746_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1079415.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2457..>2904
                     /locus_tag="IW746_002538"
     CDS             2457..>2904
                     /locus_tag="IW746_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1079416.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAH"
BASE COUNT          941 a          403 c          560 g         1000 t
ORIGIN      
        1 taaaataaaa tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat
       61 taaatagata taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca
      121 aaataataaa aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg
      181 ctatattgtt tttggagttg ttatccaaaa ataataaaaa attggagtgt ttaggataat
      241 ggataaaaag aggtttttag atcttatttc aaaaggtttg attgtttcct gtcaagcatt
      301 acctggtgaa ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc
      361 tcaaagggct ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag
      421 aaagtgtgta tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc
      481 attcattact gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat
      541 cgctcttgat tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac
      601 taaaataaaa gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga
      661 aggcaaaaat gcatataaag caggaataga ttttgtagga actactttaa gtggatatac
      721 tgaggaaagt aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga
      781 agggatttgt gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa
      841 aaaaatagga gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc
      901 atcaagattt gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac
      961 aattaggacg cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg
     1021 gactgggagg agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac
     1081 cgtggttgca tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc
     1141 tagcactaat ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag
     1201 ctggtttagc tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg
     1261 aacttttttc agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg
     1321 gtggtactgt tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag
     1381 gtttttttgg aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg
     1441 gttctttctt ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac
     1501 aaattgccca aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag
     1561 gtgctgttgg actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta
     1621 ccgaaactgt agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag
     1681 ctgatccaaa ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg
     1741 caacaatgat gtttggatta ccagcagcat gttatgcaat gtatcgtgca attcctaaga
     1801 aaaatcgtaa aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg
     1861 gaattactga accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc
     1921 atgcattctt agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga
     1981 attccttttc aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca
     2041 aaactaattg gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg
     2101 tttttacatt ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag
     2161 aagttcattc tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca
     2221 ttattgaagc tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt
     2281 tgcgtgttgc tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg
     2341 caactgctgt cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact
     2401 tattaagtca agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt
     2461 ttgaaatttt caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca
     2521 tttccatcac agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg
     2581 ccgtcatacc tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat
     2641 ttcctactaa acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata
     2701 ttggaattga tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg
     2761 atgaggttac tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg
     2821 gtaaagatac ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa
     2881 tagataatgg tcgaattgct catg
//
LOCUS       DACEND010000187         2842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEND010000187 DACEND010000000
VERSION     DACEND010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.195"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            800..1474
                     /locus_tag="IW746_002539"
     CDS             800..1474
                     /locus_tag="IW746_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1079417.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1471..1812
                     /locus_tag="IW746_002540"
     CDS             1471..1812
                     /locus_tag="IW746_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079418.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1842..2201
                     /locus_tag="IW746_002541"
     CDS             1842..2201
                     /locus_tag="IW746_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1079419.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2404..2583
                     /locus_tag="IW746_002542"
                     /pseudo
     CDS             2404..2583
                     /locus_tag="IW746_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2725..>2842
                     /locus_tag="IW746_002543"
     CDS             2725..>2842
                     /locus_tag="IW746_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079420.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGK"
BASE COUNT          964 a          469 c          605 g          804 t
ORIGIN      
        1 gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag tttttcatgt
       61 ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt tattctaaaa
      121 aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa gtttccagca
      181 gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg
      241 caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt gatgatgctt
      301 ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta acattcgaag
      361 atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa ttcacccttg
      421 aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag aagagatatt
      481 agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt ctgatggcgt
      541 catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac caaacaacga
      601 aggaatcaag gtgaatccgg cacgttaaga aaaaaatagc ataaaaaact agacacttga
      661 tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat acatatattt
      721 tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat cgttttattc
      781 aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata gcattacaga
      841 cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac ttttgattgc
      901 cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc gtgggatagg
      961 aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag ctttacaacc
     1021 tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta gcgatcaggc
     1081 tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa aattactaaa
     1141 tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg tacttcctat
     1201 aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa ttgttcgtaa
     1261 acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca atgctttagg
     1321 gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa cgattggcgg
     1381 acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt tttttagtgg
     1441 agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc agtaaaagaa
     1501 gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt aaaagcaaaa
     1561 acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac aaaagaaaaa
     1621 cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt ggatcagcga
     1681 ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga agaaatccaa
     1741 aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa atcatttttt
     1801 gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt ttaggaaatg
     1861 tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca gcggggatac
     1921 tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag atcgcctccc
     1981 tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg tcattgattg
     2041 ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat gtcgtcagcg
     2101 gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct ttcaaacttg
     2161 ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata ataaaggtta
     2221 gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc gttctcctgt
     2281 ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg tttatggaaa
     2341 gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag tggagcttcg
     2401 aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat cgaaaaatta
     2461 atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc tgtcaagatc
     2521 aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa aaagaaagaa
     2581 taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca ttttttcttt
     2641 cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga gatattcata
     2701 tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt agattgtgcg
     2761 aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa ggaagcaacg
     2821 gtaaacttca cttctggaaa ac
//
LOCUS       DACEND010000188         2750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEND010000188 DACEND010000000
VERSION     DACEND010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.196"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..482
                     /locus_tag="IW746_002544"
     CDS             117..482
                     /locus_tag="IW746_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079421.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            499..1110
                     /locus_tag="IW746_002545"
     CDS             499..1110
                     /locus_tag="IW746_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1079422.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            1138..>2750
                     /locus_tag="IW746_002546"
     CDS             1138..>2750
                     /locus_tag="IW746_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1079423.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLT"
BASE COUNT          876 a          427 c          574 g          873 t
ORIGIN      
        1 gttttattaa tgttgaattt atttgaaagg tctacagatt aacatattat tgacaaaaca
       61 atatttaaat agttatactt cattcaaacg tctgtttgaa cgaggtggtg aagttaatga
      121 ctgcagaagt atgtacaact acttgtatac ataaagaaaa agtagctagt gttaaaaata
      181 atttaaaaga taaagacctt tcaactttac ttgtattagg aaaatgtttt agcgattctt
      241 ctaggattaa aattttctat gctttagaaa cgtataagga gatgtgcgtt tgtgatttag
      301 cagcgatact taatgctagt attgctacaa cgtcacacca tttacgtttt ttgaaaaaga
      361 acggaatggc gaaatcacgt caatatggaa aggtagttta ttactcttta gcgaatgaag
      421 agattctttc cgctgtgcag gcttttctaa agttgtcaga aaatagttct atgaaagctt
      481 agtttggagg tagttatttt gttacaaaac attttatcag cactcgctgt atatatttct
      541 accagtattg attatttatt tatcttgctg attatttttt ctcaaaacca tactaaaaaa
      601 ggtctccgtc agattttttt tggtcaatat cttggaacag gaatcttggt agccataagt
      661 ttgtttgcag catatgtgct aaattttatt cctcaagatt ggatcattgg tcttctggga
      721 ctaattccga tttatttagg gattcgtgtt gcttttgtgg gcgaagagga ggaagaagaa
      781 ggggaagtcg ttgagaagct aggatctaga ggaacgaatc gtttgttttg gacagtttcc
      841 ttaataacga ttgcttctgg tggcgataat ttagggattt atattccttt ctttacctcg
      901 ttatcttttt cagaaattgt cacttcttta atcgtctttg ctatctctgt tgcggttctt
      961 tgctatatta gttataaatt tgcgaaaatt tcttttgtct ctgaaacact agaaaaatat
     1021 gaacgaatca ttgtacctat cgtatttatt ggattaggga tttttattat gatagaaaat
     1081 ggaaccatcc aaactatatt aggtttgtag agatcttatt agtaaaggaa gcaaacgatg
     1141 agtgaaaata aaaagtacca tgaatggata ttagaaggaa tcgactgtgc aaattgtgca
     1201 gacaaagtag aaagaggcgt cgccaaagtt gcaggtgttg ccaatagtag tgtgaactat
     1261 atgacagaaa ccttgagttt cgaagtgaca gaggaaaaag aatatcttgt tttatccaat
     1321 gtgcaacaaa aagtaaaaaa attggagcct gatatcactt taaaaaataa agtggacggt
     1381 aggctgattg aaataaatcc ggcattaatc aagaaaagta atttaaaaga cgataaaaaa
     1441 aactacgaat ggatattaga aggaatcgat tgtgcaaact gtgctgccaa agtggaaaga
     1501 ggagttgcta cagttcccgg cgttacaaac agcagcgtca attttatgac agagacactg
     1561 agcttcgagg tagcaggtga aaataaaact gacgtgttgg ctagtgtaaa acaaacagta
     1621 ggaactttag aacctgacgt tgttctgcga aaaaaatcgg acggtacatt gattgaacat
     1681 ggaactgact ctaataaaaa ttcgacttct aacaaatcga tttctgataa agatgagaca
     1741 aaccaaaagc agacgaatag agcttatctg gccagccttc tcggaaataa aactaaaatt
     1801 acccttattc gtttaatact aggattagga atattagcga caggaatatt tctccctatg
     1861 gatggacaag cctctcttgg gttgttcatt ctggcttatt taatcaccgg ttatgacgtt
     1921 gtaggacgtg ccattagaaa tattttccga ggtcaagtat tcgacgagaa ctttctaatg
     1981 accattgcga cgttaagtgc cttctatatt caagagtatc ccgaagctgt agcagtcatg
     2041 ctattttatc aagtgggaga agtattccaa gatattgcta tttcaaaatc tcggcgctcc
     2101 attgcggacc taatggatat tcgacctgat tatgccaatt tagtcacagg aaacaacgta
     2161 gaaaaagttg ctcctgaagc aattaagatt ggaaatatta ttctcattcg tccgggcgaa
     2221 aaagtgccat tggatggaaa agtagtggaa ggtgcttctg cgatggacac ttctgcttta
     2281 acaggcgaat ccgttccaag aagcgtgaag ccggaagaca ctattttgag cggatttatt
     2341 aataaaaacg gattgctaaa agtagttgta gaaaagccct tttcggagtc tactgtgaag
     2401 aaaattttag atttggtcca aaatgctagt ggccggaaag cacctactga acaattcatt
     2461 accaaatttt ctcgctacta tactccaatt gtggttatag ctgctgcctt actggcaatt
     2521 gttccgccat tgctagtacc aggcgctact tttaatgaat ggatatatag ggccagtatc
     2581 tttttagtta tttcatgccc ttgtgcttta gtcgtttcga ttccagttgg tttctttggt
     2641 gggattggtt ctgcttcccg caaggggatt ttagtaaaag aaagcaattt cctcgagggt
     2701 ttgaacgatc ttaaatacgt ggtaatggac aaaacgggta cattgacaaa
//
LOCUS       DACEND010000189         2730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEND010000189 DACEND010000000
VERSION     DACEND010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.197"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..264)
                     /locus_tag="IW746_002547"
     CDS             complement(<1..264)
                     /locus_tag="IW746_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079424.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPF"
     gene            complement(595..828)
                     /locus_tag="IW746_002548"
     CDS             complement(595..828)
                     /locus_tag="IW746_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1079425.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(843..1793)
                     /locus_tag="IW746_002549"
     CDS             complement(843..1793)
                     /locus_tag="IW746_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1079426.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1790..2632)
                     /locus_tag="IW746_002550"
     CDS             complement(1790..2632)
                     /locus_tag="IW746_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1079427.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
BASE COUNT          896 a          508 c          345 g          981 t
ORIGIN      
        1 aaatggatct aattcattga agttctctct ccaacttttc tctatatcta tattttctaa
       61 gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat taatatgcgg
      121 ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa aagcacccgt
      181 agttacgcct gcggtcaagc acagttgcct tatcttagtt tgctgaaatc cttctttttc
      241 aaaatggctt agtccacttt ctattaattt ttggtgagtt gtaatataat ctctggtttc
      301 tatgggtact tcctcctaat aagatgaatt aactatatta tcttacacag gacttgaagt
      361 ttcaatcgtt aacgtctatt actgaactca ttccttagag ctaaactaat gagaaagagc
      421 aaaataactg atacaaaaag caatcctaca agaattaata gactcatcta aatcattcac
      481 cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt agggtaagtc
      541 ctatcataat cactccaata gccattttct tatcctccct aaaaataaag tcttttactt
      601 aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt gaaatcctat
      661 atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg ctggactaac
      721 acttagaact tttgctaaat cattattttt taatttcttt tttctttttg ataattcctg
      781 aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac ctaccctcca
      841 aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat cgcatagtaa
      901 ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga atagatagga
      961 atattggtat cttttgatac tgttttcatt ggtctatcgt ccacactgct ttctacgaat
     1021 aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg atctggtgtt
     1081 ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata cgctttcgag
     1141 aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg aattgaagca
     1201 aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt atctagattt
     1261 tctttataga attttttatt atcaggatct ttttccgcta actgtttttc aatattttta
     1321 gcgtaaataa taccgttttc taaatttaac caagcatggg gatcttcctt ccctttctca
     1381 ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata gtctttgttt
     1441 tcctctttgt tcgcattttt tactaatttt gtaaaccaag catttcctcc agtttccaag
     1501 ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc ttcaggcaaa
     1561 ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc aattttatct
     1621 tttgctatat tttcagtaat atctgctaaa atcgaattag taactactac ctttaatttt
     1681 tcgttcgaag ccactgtatc tttgtcttta ctatttgttg aacatgcccc gaatataaaa
     1741 agagcaatca aaatccccag acttcccagt actgctttcc attttctcat cataactttc
     1801 tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt acaaataaga
     1861 atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa ccaatgaaca
     1921 aacctagcaa tgatgagcac gctccgactg tcgccgaaat aaatatcata gtttttaagc
     1981 tgtttgcata taaatatgct gtagcagctg gtgtaattaa cattgcaaca attaatatag
     2041 ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaatcatt aatagataat
     2101 gataaaaact gacattcatc cccattgctt ttgccattaa tggatcaaaa gaagtgatta
     2161 ataattcttt aaaaaataga acaactgtaa ccaacactaa aacagaaacg ccaattgtaa
     2221 tccacttatc aatatcttgt acagctaaaa tatttccaaa taagatatga aataaatccg
     2281 ttgagctatt tgcaactcct ataaggataa ctcctaatgc taaaaaagaa ctgaaagtaa
     2341 taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta ataatgatag
     2401 aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa atataagaaa
     2461 ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac attcctctta
     2521 aaataataaa acaacctact gttccagaaa caatccctat aactacagat gtaattagag
     2581 cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc atattattcc
     2641 cccttgtata ttagagttag ggttttaacc ttccggtttt gattggataa ccctatgaac
     2701 catcgttatt ttaatttggt attaactcat
//
LOCUS       DACEND010000190         2708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEND010000190 DACEND010000000
VERSION     DACEND010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.198"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            240..656
                     /locus_tag="IW746_002551"
     CDS             240..656
                     /locus_tag="IW746_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1079428.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="IW746_002552"
     CDS             670..1236
                     /locus_tag="IW746_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1079429.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="IW746_002553"
     CDS             1259..2245
                     /locus_tag="IW746_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1079430.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2708)
                     /locus_tag="IW746_002554"
     CDS             complement(2400..>2708)
                     /locus_tag="IW746_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1079431.1"
                     /translation="DSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSD
                     NRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR
                     "
BASE COUNT          824 a          504 c          560 g          820 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 tcacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct
     2701 gtactgtc
//
LOCUS       DACEND010000191         2651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEND010000191 DACEND010000000
VERSION     DACEND010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.199"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..247
                     /locus_tag="IW746_002555"
     CDS             <1..247
                     /locus_tag="IW746_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAQ1079432.1"
                     /translation="KPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVL
                     HLVRSTYNLENEVIEFTLSVARADKFHYKIRHLRSTN"
     gene            263..1432
                     /locus_tag="IW746_002556"
     CDS             263..1432
                     /locus_tag="IW746_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1079433.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            1448..2443
                     /gene="lacD"
                     /locus_tag="IW746_002557"
     CDS             1448..2443
                     /gene="lacD"
                     /locus_tag="IW746_002557"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1079434.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            2522..>2651
                     /locus_tag="IW746_002558"
     CDS             2522..>2651
                     /locus_tag="IW746_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1079435.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCP"
BASE COUNT          854 a          474 c          610 g          713 t
ORIGIN      
        1 gaaaccttta tataatttgt tttcagagaa tttcaatcaa atcgtacggt tggctgacga
       61 ggaattttat gccagcattg tccagaaaga agatagtgag ttgctaaata ttccagaagg
      121 atcaccagtg ctccatttag ttcgctcaac ctataatctc gagaacgaag tgattgaatt
      181 tacattaagt gtagcgcggg ctgataaatt tcattataag atcaggcatc tgcgttccac
      241 taactgaatt ggaggaaaga aaatgtttat gttctcaaaa gaagaactac gaaagctagg
      301 agcagaaaat actacccgag aaattcgaca acaaccggaa ctgtggcatg agacgtttga
      361 ccgttatcaa gaaacagttg cagatttaga tcgtttgttt acggatgcga aacaacatgc
      421 tggagagcga ccaatccgag tcatttttac aggggccgga acctcgcaat atgtgggaga
      481 tacactaatt ccgtttctta ctagtcaagg aaatacgaac cagtttattt ttcaaagtat
      541 tggttcgacc gatatcgttg ctacgccaaa agaatacctg attcctgatg agacaacgat
      601 cttggtctca tttgcccgta gtggaaatag tccagaaagt ctagccgcag ttaagatggc
      661 caatcagctg gtcaacaaga tctatcatat cgtgatcacc tgtgcaccag acggattatt
      721 ggctacacaa atgcctacaa aaaaagaaaa tctagtttta ctaatgccaa acaaagccaa
      781 tgacgctggt tttgctatga caagcagttt cagctgtatg acgctgactg cgctacttat
      841 ttttgatcaa gcaagcaatc agcaaaaaga aagatatgtt catcaattaa gccgactagg
      901 agaagaagtc atccaacgag aatcagagat ccaagggtat ctggcatcag actttcatcg
      961 catcatttac ttaggttcgg gaagcttagg cggattgact agagaagcgc aattaaaagt
     1021 tctcgagtta actgctggaa aaatcgcaac cgtatttgat acatctttag gattcagaca
     1081 tggtcccaaa tcttttgtca atgagcaaac attggttttc ctgtttgtca gcaatgactc
     1141 atatacgcag aaatacgatg tagacatgct ggaagaaatc aaaaatgacg gtattgccca
     1201 acgcgttgtt ggaattggtc aaaagggttc attgtttagt ggtgattcct ttgaattagt
     1261 tgcagattcg ttactgccgg atggctatct tgctttagca gatatcatgg tagcccaaac
     1321 gatcgcatta ctttgttcgg tgaaggttgg taatacacca gatacaccat cgccaagtgg
     1381 aacagtcaat cgtgtggtca aaggcgtaac gatttatcca tatgaaaaat aggaaagagt
     1441 gtgaagcatg ttagaatggt cggaaaataa aagacaagca atggaacgtc tatcgacgcc
     1501 agaaggcatt attaccgctt tggcgatcga tcagcgaggc gcgctgaaga aaatgatgtc
     1561 ggctttaggg atggaagcga caaaggaaaa aattgagcag tttaaaacac taatttccca
     1621 agaattgact ccttatgcct cagcaatttt attggatcct gaatacggac tgcctgctgc
     1681 acagaagcgt gcagcagcga gcggtctgct gttggcatat gaaaaaactg gttatgatca
     1741 aaccgtaccc ggccgcttgc cggatctgct gtcggactgt tcagttctgc gattgaaaga
     1801 gttaggtgcg gatgcggtga aatttttact gtattacgat tatgcagaag cagaggaaat
     1861 caatcggcaa aagcaagttt ttgttgaacg aattggaagc gaatgcatgg ctgaaggact
     1921 gccattttat ttagagttat tgtcatatga tgctaaaaat gtagatacga aatcgctttc
     1981 ctatgccaaa gcaaaaccgc gcaaagtgat cgaaatgatg aaagaattct cgaaaaccca
     2041 gtataaagta gatgtcttaa aggttgaagt accagtaaac atgtcatttg ttgaaggaaa
     2101 tgcaacaggg gagatcgcct atacacgtca agaagctgcc gcttatttta aagaacaaag
     2161 cgatgcaaca gatttgccgt atatcttctt aagtgctggg gtatcagcgg cattattcat
     2221 ggagacattg cgttttgcca aacatgctgg ggcacgattc aacggtgtac tctgtggcag
     2281 agcaacttgg aaagatgcag tcgctccttt cgttgaaaaa ggagaagcgg caactttaga
     2341 atggctgaca gaacaaggga cacaaaacat tcgtcaattg aatgaagtga taagagaaac
     2401 tgccatccca tggtatgaga aagtaagtga aagcacttgt taatgtttct aatatattag
     2461 tagtgataag agatatgtgt gattataatt tatcattatt gaaactggga ggatatttta
     2521 tatgaataaa agattgttag attattcagc ttcagatttt ttgaaagcta agccgatgga
     2581 attaaagcag gcaattttgg cttctgaagg tagaacaatt atggcagaaa atgtgccaag
     2641 tagctgtcct t
//
LOCUS       DACEND010000192         2624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEND010000192 DACEND010000000
VERSION     DACEND010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.200"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(206..1255)
                     /gene="recA"
                     /locus_tag="IW746_002559"
     CDS             complement(206..1255)
                     /gene="recA"
                     /locus_tag="IW746_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1079436.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1364..2605)
                     /locus_tag="IW746_002560"
     CDS             complement(1364..2605)
                     /locus_tag="IW746_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1079437.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          691 a          594 c          463 g          876 t
ORIGIN      
        1 aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat
       61 tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata attaaataac
      121 tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac
      181 cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt
      241 tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta actagagcag
      301 aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt
      361 gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt
      421 ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta
      481 cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt
      541 taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg
      601 ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca aacatcacgc
      661 ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac
      721 cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac
      781 ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga
      841 tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt
      901 caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat
      961 ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa
     1021 tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac
     1081 gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa
     1141 ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc
     1201 tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga
     1261 taatcctcct tatatttaca aatcagagca acatttcatt atccttatca tatctttttt
     1321 agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct
     1381 gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc
     1441 tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg
     1501 ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt
     1561 aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga
     1621 ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga
     1681 gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg
     1741 cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt
     1801 tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt
     1861 cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa
     1921 catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga
     1981 tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac
     2041 caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct
     2101 ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa
     2161 gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat
     2221 ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt
     2281 ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga
     2341 gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta
     2401 atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta
     2461 aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct agatcagcta
     2521 gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc
     2581 cgacagctat tatttctgct ttcattactt cccgctccct tata
//
LOCUS       DACEND010000193         2527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEND010000193 DACEND010000000
VERSION     DACEND010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.202"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW746_002561"
     CDS             complement(573..1130)
                     /locus_tag="IW746_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079438.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW746_002562"
     CDS             complement(1309..1920)
                     /locus_tag="IW746_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079439.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW746_002563"
     CDS             complement(2033..2290)
                     /locus_tag="IW746_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079440.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          750 a          537 c          326 g          914 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaa
//
LOCUS       DACEND010000194         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEND010000194 DACEND010000000
VERSION     DACEND010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.203"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            115..822
                     /locus_tag="IW746_002564"
     CDS             115..822
                     /locus_tag="IW746_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1079441.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            809..1132
                     /locus_tag="IW746_002565"
     CDS             809..1132
                     /locus_tag="IW746_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1079442.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1221..2255)
                     /locus_tag="IW746_002566"
     CDS             complement(1221..2255)
                     /locus_tag="IW746_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1079443.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
BASE COUNT          654 a          524 c          450 g          856 t
ORIGIN      
        1 gctagttctt tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt
       61 cacaagttta acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat
      121 gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc
      181 ggtattggac ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc
      241 acgttgatgt ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt
      301 gctagtcaca gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg
      361 attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg
      421 ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat
      481 actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg
      541 tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag
      601 tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt
      661 tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg
      721 gtatatgtcg gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt
      781 gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat
      841 tttaggttgt actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa
      901 attcgattta ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc
      961 tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct
     1021 cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt
     1081 ggtgatcgtc ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa
     1141 acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa
     1201 aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac
     1261 atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct
     1321 gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc
     1381 gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac
     1441 tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc
     1501 cctacgatcg ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag
     1561 cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa
     1621 cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat
     1681 tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga
     1741 ttcaaaatcg aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg
     1801 gcattttctt ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag
     1861 gtcaacggca aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata
     1921 cgttcgtcaa ctagttggta ttcttgattg cttcctgctc ttgttgtacc tgcgtaaaaa
     1981 acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact
     2041 ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta
     2101 tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca
     2161 ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa
     2221 aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa
     2281 gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt
     2341 tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa
     2401 aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc ctaccaactc
     2461 ttcggtcaca agtcaaaata ttca
//
LOCUS       DACEND010000195         2458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEND010000195 DACEND010000000
VERSION     DACEND010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.204"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(899..1417)
                     /locus_tag="IW746_002567"
     CDS             complement(899..1417)
                     /locus_tag="IW746_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1079444.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          801 a          449 c          355 g          853 t
ORIGIN      
        1 ataaaaatac taaagttatt tgttctttta ttaaatacac ctacaactta taaattgatg
       61 gtatttagat aacacgaaag aataaaaata acagctgata gtaaagtttg ttgacgattg
      121 ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa
      181 agacctttta catcttgcag aaatcggtac tgggattaaa ctaagcaata aaaactaatg
      241 ttcccctcat tctttgaact tctactattt gatttattta ttctgataaa tgacgatttc
      301 accttcagga tcttcaaatc ccgtatagaa tttatttaag atctcttcag tatcagtaat
      361 aggataggcg ataggccaca taccctttga tggttagaat aaactgctca aaatctgtat
      421 cgtatgttgg ctttagcata ttaacaaagg ctatctccaa attataaacc ataagcgttg
      481 aatcgttctt aacaatttca attcttccta acccaaagtc agaagatata attaccggac
      541 ttgccttacc cttcatgtgg gttgttgaga ttccatattt aaagggaatt gtcggtacta
      601 tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag tctcctaagt
      661 ccctttgtag cggatatggc accataatct cattttatcg aagtgagtca tggtttatct
      721 gttggccaac caaaattgat acaatctaac gatgcaccct ctacggcatt ctaggggagt
      781 gcacttgcat tcacccaata aaaaccggca gttagcaaag aacttatgta gtcgtatgtt
      841 cttactgtta acatacaaga ggtctccttt ccacaattgc ttagcagtaa acgggaattt
      901 acaaagctaa tctcttccat tttcttatct ttgttctaaa agtcccaaat ggagcaactg
      961 tatttacatg aataaattta tatacttccc atacagccgt tttggttgcc tcgtctgccc
     1021 attttctttt gtgtgcagtg aatagatctt catcactcat tgaatcaatc atcatgttaa
     1081 tatcatcaat attacgattg agaatttcct ctaattcttt caatggtaga tgtgcatagg
     1141 tatttgtaaa ccacttatat aactctccta actgattcca tttaaattga tcagaaggcg
     1201 ttttaacaga taatccattt ctttcatcat tttcccattt tagaattaaa ttcgtccaac
     1261 caacttgata tgctaaattt tcagcaggtg ttctatccac ttcctcaact cttttatcct
     1321 tcaattcttc aggaatagtt tcaaattcta aaatatattt agtataagtc ctatttattt
     1381 ctttttttaa ttcttcttta tttttatatg ttctcattat attacctcca taaattccta
     1441 attgtctcct ttaattcatc tataatttca tccacgatat tttaatccgt tctacaatgc
     1501 atatctgata tgcattgact gtctaactca tatgtgaatg atttataaaa tatcccaacg
     1561 tctcatctaa agaaatgttg atcgtcagca aggagctaaa aaatacgaat atatttcttt
     1621 tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa
     1681 ctactatcaa ttactaataa aaactaccac tttaagcgtt aagaactgtt aagtttagtt
     1741 aagtgaggat agttcacaaa atccattgtt aaacttcacg aaagtggtaa aactcaaaat
     1801 gctttatata ggattacagc atcgctcttt cttcgattgc gcgttgggta aaaaatatta
     1861 tgaagtaaaa attgacgatg acacgttcct tacggctcat cagatgaaac agctacaaaa
     1921 acgaaatgat caactcgaag aaaatttaat tctaaaaaag cgattgccat attcacgcct
     1981 cgctcgacga actattaaag gtcaagattt acctgaagat catccttttt aggaaattga
     2041 agctttttat gttgcacaat gcatttataa tactacaatg atgttttcac cagatattct
     2101 tctagggtgc ggtgtaatga aacagataca cttgcaaaag aaaattcacc gcgaatttac
     2161 taaaattatg aataattatg ttaatcttcc gaaatttttt tccaaaatta agggatcaag
     2221 ctggtgttat agattgttta aggttggcaa aaaaataact tctttttaaa caattataga
     2281 aaaccgatac tctgttaagt tggttgacac ggtgtcgatt tttatgactt tcttattctg
     2341 aaacttcact aacggacagt acctaaaaag tctatagtat ggacgtttac gagtactatt
     2401 ttatcaaaaa tgctgctcat ttcaacttat atgttttagt gaactacccc cacttagc
//
LOCUS       DACEND010000196         2414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEND010000196 DACEND010000000
VERSION     DACEND010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.205"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            188..511
                     /locus_tag="IW746_002568"
     CDS             188..511
                     /locus_tag="IW746_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079445.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="IW746_002569"
     CDS             675..929
                     /locus_tag="IW746_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079446.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="IW746_002570"
     CDS             999..1244
                     /locus_tag="IW746_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079447.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1341..1685
                     /locus_tag="IW746_002571"
     CDS             1341..1685
                     /locus_tag="IW746_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079448.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="IW746_002572"
     CDS             complement(1746..2309)
                     /locus_tag="IW746_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079449.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          717 a          408 c          386 g          903 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgtgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggt
//
LOCUS       DACEND010000197         2381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEND010000197 DACEND010000000
VERSION     DACEND010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.206"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..697
                     /locus_tag="IW746_002573"
     CDS             <1..697
                     /locus_tag="IW746_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1079450.1"
                     /translation="KNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSV
                     KYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMF
                     LRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAI
                     TNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELR
                     EKVLRSIIDKSIE"
     gene            927..1157
                     /locus_tag="IW746_002574"
     CDS             927..1157
                     /locus_tag="IW746_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1079451.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1380..1478)
                     /locus_tag="IW746_002575"
                     /pseudo
     CDS             complement(1380..1478)
                     /locus_tag="IW746_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1743..2198)
                     /gene="tnpA"
                     /locus_tag="IW746_002576"
     CDS             complement(1743..2198)
                     /gene="tnpA"
                     /locus_tag="IW746_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1079452.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          754 a          407 c          381 g          839 t
ORIGIN      
        1 gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa ttggtttttc
       61 tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa aaaaagaaca
      121 tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag ctctctattc
      181 cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga tcactcaatc
      241 aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg atttagaaaa
      301 tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt ttcaagccca
      361 agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat ctgggaaaac
      421 gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa ttattgctat
      481 aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac aaacagccat
      541 tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt atatttgtga
      601 tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat taagagaaaa
      661 agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt agctaaacct
      721 aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt gatagcttat
      781 tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct acggttcttg
      841 attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag aaaaacgtgt
      901 agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct ctcttaaata
      961 aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag gaacctactg
     1021 aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg ttttctataa
     1081 agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt gtttataaga
     1141 acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact tcaatgttat
     1201 gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta aaagtttatg
     1261 atccttttga tttttatatt aatttatctg tatttaatac agattttatt tattagttca
     1321 tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac tcttaattgt
     1381 caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc caaaggtgtt
     1441 cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct aatgtttgaa
     1501 tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa attgccaaaa
     1561 tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt ctatcagtaa
     1621 cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc gacactcgct
     1681 gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc acttacttct
     1741 ctttattttt taaatggttc tacgtactct cttttactta taatatatcg aatgcgatca
     1801 tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc gactgtactc
     1861 acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag atttgcgtgc
     1921 tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact ggatatacat
     1981 atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg tgcttctatg
     2041 atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc ttttcgtaat
     2101 tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata cttacaattc
     2161 caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct ttcgatctga
     2221 tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct aaagatactt
     2281 cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac aaaactgact
     2341 gaagtcatta agaaaataaa agggtctaaa acttttaagt t
//
LOCUS       DACEND010000198         2373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEND010000198 DACEND010000000
VERSION     DACEND010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.207"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..925
                     /locus_tag="IW746_002577"
     CDS             <1..925
                     /locus_tag="IW746_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1079453.1"
                     /translation="SSKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMS
                     KNTILSALDELQKVKLLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQE
                     NRQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSK
                     DIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQF
                     DNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHR
                     IKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            1001..1219
                     /locus_tag="IW746_002578"
     CDS             1001..1219
                     /locus_tag="IW746_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079454.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1206..>2373
                     /locus_tag="IW746_002579"
     CDS             1206..>2373
                     /locus_tag="IW746_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079455.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIK"
BASE COUNT          900 a          309 c          413 g          751 t
ORIGIN      
        1 aagttctaag ttagtttata tgttactaaa agatagaaat agactttcat tgaaaaataa
       61 cttgtatgat gagaatcaaa aaattttttt gatatttgaa atcaaaaatc tcgtagaaat
      121 ttcgggaatg tcaaaaaata caatcttatc agcactagat gaacttcaaa aagttaaact
      181 tcttgtgaaa agaaaaaatg gcttcaataa aagtaaaatg caaatgttcc caaattttta
      241 ttatttgtta aagcctgttc tgtgcaaaga tgatgtttat aaaattaata agataaccca
      301 tcaagaaaat agacaaaaaa acttgatagt tcaaaatttg aactatggaa caaatcaata
      361 ttctgttgat agttcaaaat ttgaactatg cgacacatca atgaaaacaa gagacaaaaa
      421 caattttgct gatagttcaa aaaatgaact agaattatat aagtcttata aagatagtaa
      481 agatattaaa gaatcacata atgatgatct tcttgaatca ctttcaacta aaaataataa
      541 attagatgaa gaattgatcg aaacatttat tgaagaaaat cttcttaaac aaatatgggg
      601 agaaagtatt gtgaattgct taaaactggc tagcaatagt gattatcgtc aattcgacaa
      661 ctggtacaaa aaattcaaat atgcgattag atctgctgaa aaagaacaaa aaattcaact
      721 gaaaattatc tatgaaattt gtaataacaa gtattttgta gatcatgtta gagaacagct
      781 atcaatgact ttacgtgact tgatacgaag agcaaaaaca gatcatcgta tcaaacaaaa
      841 agacgattat atctttgctt ctctaaaaaa taaagcgtta gaactaattg aattgcaaat
      901 cagcgaagga atagataagc aataagaata gaattgagtc aaatgaaatg gaggggtatc
      961 tccacttcat ttgagaactt tgaatatagg aggggttatt ttgattaaaa atcgtttaat
     1021 agatcaagtt tggtgtctag atcaaaatac agatagtctc gaattaatcg agactatttg
     1081 cttcaacact atagttctcg atttaagagc tgaaaatgat aattgtgtaa ctcatgtcat
     1141 aattaatcaa aaaatggctc agaagctctc tgagagcctc agaaaatggg ctaagggagg
     1201 taacgatgaa aataattgaa aaaaagattg ccaataaaaa aataaattgg ttggttgtta
     1261 attttcttgt tctgatagct atgattattt ttacaagtaa tattgctcat gcgagtattt
     1321 tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac
     1381 cttacctaaa aaagtacaat ggagatttcc ttagtcagtt cgggatttgg ctcggatggg
     1441 ctgtggtaaa aggcatgttt actgtgacag attcgattca aaatatgatt ccagatgtgc
     1501 tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagccgtaa
     1561 atacaattgt cattggatta atgatccttt cattgatgtt tgttggttac aaaatgatta
     1621 ctggaaaagg aacagtggat ttaaaatcgg ttggaatgaa cgtagtgatg tctatagcat
     1681 taatactttt aatgccaacc atgatttcta gtggtattca atttgctaaa acattttata
     1741 aggactctac tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag
     1801 gagtaacaga tttagcatat attaataaaa cagatcagta ctcaagcatt gataaaacgg
     1861 aggatcgaaa taatttaaca cgaaaaaatt ttcaacagac ggatctcaca caagtgttaa
     1921 cggataaagt gattgataaa ttggaaaaag aaaatccaat tgcggacaat cttcgttatg
     1981 aattagttga aaattctaat aatgaatttg ttgctacaaa gttttctgac aacttcttat
     2041 caacattttc tgatagcttg aaatcaggct attaccgata ccaagcaaat ctatggggga
     2101 tttcaattgg gttgacagcg ttagcaatcg cctatgtgtt cagcgcgttt gtgatcatta
     2161 cagcaatact agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcaacagata
     2221 ttgagacagg acagcgttca aaagttgttc tatcagatat tctacaatgt tacttaactg
     2281 ttgggtttca aggatttggg ttatcaatgt ttgctatgtt tatcaatttt ctgaactcag
     2341 gacaaggtat ctcaacaaat atttttataa aaa
//
LOCUS       DACEND010000199         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEND010000199 DACEND010000000
VERSION     DACEND010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.208"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..1293)
                     /locus_tag="IW746_002580"
     CDS             complement(127..1293)
                     /locus_tag="IW746_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1079456.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1299..1988)
                     /locus_tag="IW746_002581"
     CDS             complement(1299..1988)
                     /locus_tag="IW746_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1079457.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2115..2354)
                     /locus_tag="IW746_002582"
     CDS             complement(2115..2354)
                     /locus_tag="IW746_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079458.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
BASE COUNT          783 a          448 c          345 g          795 t
ORIGIN      
        1 gacgattaaa gcgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt
       61 cagacaaaac aaaaaacact ttttcacaac gaaaaagtgt tttctcttat tactattcta
      121 tttcatttat ctggtggttt tatattggtg tgtagccatt gctaacatta agaacacaat
      181 tggtacccaa ctgattgtag ctaatgtttc ttccatgaaa gattgacaca tgatcgctaa
      241 aaaagcccac aagaatgtta gttgtccatt tgtcagctca cattttctta cgagcaacca
      301 taagaaacta agcaaaagaa tcatcgatag agcaaaacca atcaatccat agtgattgac
      361 gagtgcgtga tacgtattat ggttacctag aatctgattt cctctatgga aaaagaaagg
      421 tttcatccct acaaatattt gtcctgtttt ttcgcctaat gtttcataga atttgcgcca
      481 aatttcttct cgtcctgtaa ataaatgaac tttgtctgac attgcagcaa aataagaaat
      541 cggtaaagct agaaccgaaa ctgctgcata catccaaaag gacaagtgtg tgtttcgaaa
      601 gatcttctta ggtaaaattt ctaggataac gaagataaca agcgcgtata atgctgcttt
      661 cgattggcaa acccaaatcg ttgccccagc aagaatcaac gctggtaatg atagcaatcg
      721 catgtaccat tttccaaatg aactagcaaa tcctgtgata agaagggcta atgtcattaa
      781 agaactacct atcgtatttg tattgatcca aatatctgat aatttgttcg atggattcca
      841 aatactttgt ccttctggta ctggtatctc ggtaaatact cgataaaccg ttacgagtaa
      901 aaagacatag aatactacaa gatacaagct acgatcaaat acagtaaatt ccgtccattt
      961 tacaatcaaa ataatggcaa taattgtcgg aacgatcaga tcaccataga aagtatctcc
     1021 tcggtagctt ctaaaaaaag aaaagaaaag gtaaaaaaga atgcctgctg caatgaagaa
     1081 tagatcgttt ctctttacct tgtacagatt caaaagaaac aaacagccta caaaaaacgt
     1141 cactaactgt gcagcattat cataaataaa taatatcgta ggaggattta accccaccgt
     1201 ccatattaaa taaagaatgg ataccaacag taacagaaaa atggcactca ttttattgat
     1261 caatttttct acaagttctt gaatgtgact cacttccctt atttattgat tttttctaca
     1321 ttgactaatt cgcactggat cacatcgcac tttttcatat tatttgcttc aaagtctaac
     1381 agtgtgagta gtggtttttt acctgctttt ggcaaggata aaaaggaatt gttttcctta
     1441 gattgaacta cctggctgta atcataaatt tttcctcttt tggagacttc atactgatac
     1501 ttgtaatgag catcgattag atcaatttct tctccttcgg ataaatcaat catatcggat
     1561 aataaaacgc ttggtgtatc tacgttataa ccagcaagaa tataatttcc atttcccatt
     1621 tcagcatctg gataataagt agaagctact cctaaactaa gcgtgtcgct attcgctcct
     1681 tgaaaaacag ggagaataaa tcctgtagaa gaaaaaacaa gttttccagc aatttcacct
     1741 ttgagatcct tttctaatgc ctgtttttga ttagtaatca gttgtctctt agaaatatcc
     1801 gttaaactgg ttaaattatc ttgataggtt ttagcctgtt cctgaatatg gattgttttt
     1861 gttacatcat ctactttttc agcgattttg tccttttgat aaccttgata agcaaaataa
     1921 ctaaaaagga gaaaaacaat gattacgccc acgtaaggat taaataaagt tttattgttt
     1981 ttattcatct atctaccttc tatatattaa ttaatttacc tacaactaac caagataaac
     2041 cacgttaaat tttactattc tttttattca ataacaatgc cacatcataa ttgatcattt
     2101 tcatatcata atcgtcaaga aacttctaat aatagttggc aattagaact tgttttcaca
     2161 taagttcgtc tcaactgttc aaaatttttc attgatccca atagacaggc tttctccttt
     2221 cctgttatct gcatatcatt gatatattgc atgacagata atcttttaag gtgtttttgt
     2281 ttcatcattt ttagcaactt ttcttccatt tcttttgaca cttttttcca atttacgctt
     2341 tctttacaat tcatgagttt tcctaccttc c
//
LOCUS       DACEND010000200         2366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEND010000200 DACEND010000000
VERSION     DACEND010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.209"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..605
                     /locus_tag="IW746_002583"
     CDS             <1..605
                     /locus_tag="IW746_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1079459.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IW746_002584"
     CDS             739..2082
                     /locus_tag="IW746_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1079460.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2194..>2366
                     /locus_tag="IW746_002585"
     CDS             2194..>2366
                     /locus_tag="IW746_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1079461.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          938 a          301 c          422 g          705 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aatctttaaa acttgcatca
     2221 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     2281 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     2341 gtaacagcag agagtagtga actttt
//
LOCUS       DACEND010000201         2336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEND010000201 DACEND010000000
VERSION     DACEND010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.210"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..621
                     /locus_tag="IW746_002586"
     CDS             46..621
                     /locus_tag="IW746_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079462.1"
                     /translation="MDDEDQSLLKELANLNYRVEVKTAKGDYQAIKAKVIKVSDDYFA
                     VKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIHEDKVKDHVKDEDYEQHAIN
                     YKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLPYQVAEDQENTENKINSYQQ
                     EIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            644..873
                     /locus_tag="IW746_002587"
                     /pseudo
     CDS             644..873
                     /locus_tag="IW746_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1032..1967
                     /locus_tag="IW746_002588"
     CDS             1032..1967
                     /locus_tag="IW746_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1079463.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            2011..2280
                     /locus_tag="IW746_002589"
     CDS             2011..2280
                     /locus_tag="IW746_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079464.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          882 a          321 c          460 g          673 t
ORIGIN      
        1 tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgattga cgatgaagat
       61 caaagcttat taaaagaact ggctaatctt aattatcgcg tagaagtaaa gacagctaaa
      121 ggggattatc aagcaataaa agccaaagtg attaaggtgt ctgatgatta ttttgcagtg
      181 aagctatcgc atgtaccaga agaatatatt gctatgcgat taactattat tcctgaaaaa
      241 attgatccaa aggtggatat gcaagaaccg caagatctga ttttttatat tcacgaagat
      301 aaggtcaaag atcacgtgaa agacgaagat tatgaacaac atgcgatcaa ttacaaagtg
      361 aaaggataca agaaagaaca aaaagcagca caaaaacaaa ttgaatcctt ccaagcaacc
      421 attgatttga atgaagaatt agtgaagaaa ctaaaagatc aattgcctta tcaagtcgca
      481 gaagatcagg aaaatactga aaataagatt aatagttacc agcaagaaat tgaaacatcg
      541 aaaacgcaaa tgaaagatca agaagagagc attcaaaaac ttcaagaaaa aattgatcgt
      601 atgacaaaag aaaatattta gaagaatagg tggagtaaaa gaaatgaagc gagcgaactt
      661 ggtcggcaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
      721 aaaagctaaa gaaatatgtc aagaagtgaa ttatctaaca caacaatatc tgagttgaag
      781 aatggtagaa agagtttaga taccttaaaa ttcagatcat ttgaagctct atatatagct
      841 gctaaaaaat attttacagc tgataataaa taaaaaatta aggtgggttc tatgtgaaaa
      901 aaacgtatca aaagttttta tggattagct atatcgtcct atttatcgtt gggtttctcg
      961 tcagtgtgtc cgtgatcgtc ggaacgcttt ttcttttgtt ctctgacgaa gatctggtac
     1021 ttaaggggtt aatgagtacg ttatcgtgtg tcatgttact ttatttatgt atacagatca
     1081 acgaaattat gccccctcat tggcaagaaa agaagaaaga atttaaggaa gaaatcgaaa
     1141 taaaatccaa tgactggatt gaatacacat ggcttcctct gaaaagaaat agcaaacgaa
     1201 aggaattttg ggcattgcaa ttatggccaa taggaaaact ttttatttat atggcttcac
     1261 ttatggttac tttaatcggt tatttggtgg tggtctatgg ggtggcctat tacttgttaa
     1321 gtgagccaaa acagatcgcg gcgtcaaata ttttcacggc agtaatgaca ttaatgtgtg
     1381 tcatgtgctt ttctattttt agttacttac tcgtccaatt tatcaggcaa aatttaacaa
     1441 tgagtaaact tttcaaaagt ttattggact tacttatctt aggcagtatg ttaacgactc
     1501 taacagatcg gttcgaacaa tctgaaaaaa cggtagaggg gataaagaac ttcttaacga
     1561 cgattgatca atttataaat ggatatatgt atttgtttga agtaggcttt attctcactt
     1621 tgtttggttg tgtatttata aaactcatta aaagatcaat cgaaaaagaa atggttattg
     1681 atctaattcc gacgctgagc ggctatgagt attacgttga ttttggaaag tatgaggagt
     1741 tgatccatac ggatatcgtt cacacgagta tccatgaagt aaccgttaag ggcatcagat
     1801 ctactttaaa agatggacag gagacgaaaa caatcgaccg aagaaaagag gtctacaatt
     1861 tttataaagt tgaagcaagt ctacgatatg gcattgggaa attgaggtta gatgggagta
     1921 atattcaaca aaacaaacca tatgttttag gaaaagtggt gatttagaac ttcctatgaa
     1981 agatgaataa ataaggagaa tcttttgaaa atgattcttc tttacagaat tgtgcttata
     2041 gttggcacgg agttaaaaat cttaaagaag gagcagttat tcaaagtgaa agaacatcat
     2101 aatcagttac tatgtattaa aacgggtacg atgaacattc aataccgaac agaatattta
     2161 gaattaacga atcaaactga ttttttgtcg attcgagagc aacataagca agaaaaatta
     2221 atggctactt atagccaatt gattgatgag tgggaacaat tcaaggaatc taaaacataa
     2281 aattccattt aaacacctta ttttttaaag agcataagga aacgtgaccc cacttt
//
LOCUS       DACEND010000202         2320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEND010000202 DACEND010000000
VERSION     DACEND010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.211"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..1097
                     /locus_tag="IW746_002590"
     CDS             201..1097
                     /locus_tag="IW746_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079465.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            1146..>2320
                     /locus_tag="IW746_002591"
     CDS             1146..>2320
                     /locus_tag="IW746_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079466.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLE"
BASE COUNT          906 a          324 c          434 g          656 t
ORIGIN      
        1 ggttgaattg atagatggac tcaaagaaga aaattcaaag ttaaaagaga agataaaata
       61 ctatgaatgt ttatttgatg gtatagagag tctgctgaac tcacgaaaaa atccctagtt
      121 tttatgaatg accgttttgt aaatctgatt gaaatagatt tatatgattt cggtatatga
      181 tatacattag gaggtgtcga atggaaataa atctttttga ggctggtaat cgaataaagc
      241 aaatacgaga aaaacatgga tatactatgg cagacttagg taaactggtt gatgccaata
      301 gtcctagcac aattaacaat tgggaaaagg gaaataactt acctaataga aagcgtcttg
      361 aaaaaattgc actattaggt ggtacttctg ttgaatggat aaagtatgga gatttttccg
      421 attatgttta tcgcttgaca caaaatatag aggaaaaaat tcttaaagag tacggagaga
      481 ctacactgaa caactaccgt gaagctcttc tagatgaact tacgattgaa aaaatcacat
      541 acgaagaaga tacaaagata ttagcagctg ccgaaaaaat caatacacaa aatatttttc
      601 aggaggtgaa gtacatgtat tctcctgatg aagattatcg aaatatagaa gtcaaaccag
      661 atttaggact gactgatgac aacaaaaata ttaaaatgat tggacaacgt ataaggaaaa
      721 taagaaaaaa agagaatatt acattagaag aattcggcaa actattctct cctacagctg
      781 ataaagctgt tataagcaac tgggaaaatg gtaaaaacct tcctaactca gaaagaatta
      841 aaaagattgc agaaattggc ggagtttctg aactttatct tatgacgggt gtcgattcaa
      901 gcgtagcaga atcaatgtat tttctatctg aagtagcatt ggatgctttg gaacgtctgt
      961 caattgagga agtcaataga ataattgaaa gcttcgccgg ttatcttaat gtgatttcaa
     1021 aaattgatga tccagataag cgagaaatat ctctcaatgc tatttgtcaa ctatcacttc
     1081 tcgataaaat gggttgattc acaaaaaata ttgaaagatt actttactca cgaggaggct
     1141 tagctatgaa acctaatcta tcgttaacta gtgaacgtat tagaaaaatt atcgaatcca
     1201 aaggtctgac attagaacga ttcggtgatt taattggcga cacaccaata agttctgtta
     1261 ataattggct tagaggcgtg tctttaccca ataaaaatag gcttttaaca attgcaaata
     1321 ttggaaacac tagtgtagac tggattaaat ggggaacttt ggaagaatac atagcttcat
     1381 accttataga ttcaggttat gagctatata ttaaagactt tccagagata ccttacaaag
     1441 tctttaaaga cattcaagaa aaatatagtg atacgttttc actagataaa gactatgagt
     1501 tattgaatcc tataataaaa tttactatag ggggcgttac tttgaataga ataaaatttt
     1561 atattgattc cagtaaaaag accattaaac aaattagtac agaaactggt attaactata
     1621 caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa acatggaata
     1681 tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac gaaacaaatg
     1741 ttgaactcaa aaaccttaat attggaaaca acatcaaaaa tattagaaaa agtaaaaata
     1801 tgacacaaac tgattttgct gagttaatgg gtctatcacg atcatatata ggggatttag
     1861 aaaacaacag gagtaatccc agtataaaaa cactggaagc tattgcaaga gcattggacg
     1921 ttccgatagt agcattaatc aacgattctt ctactgataa tatagtagag ccgtatgaaa
     1981 gtttgacagc cgaaaatatc cttcttaaac aagaaaatga ggaattaaaa tatgaggtag
     2041 aaagcttacg aaaaagattg tatgaaatcc aacaagtagt aaatcaagga gctgaaaaaa
     2101 tgattcccaa tctaaaagac gtaagtaaaa gaatcaaata tatcagaaaa aaattaggta
     2161 tgactatgga gcaatttggt tctcatttaa gcaactcacc taaaagtaca attgctactt
     2221 gggaaagtgg tagaaatata ccttctcaga aaaagttaca gctgattgct atagtaggag
     2281 aaaccaccac tgactggatt aaatggggaa ctttagaaga
//
LOCUS       DACEND010000203         2247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEND010000203 DACEND010000000
VERSION     DACEND010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.212"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..558)
                     /locus_tag="IW746_002592"
     CDS             complement(<1..558)
                     /locus_tag="IW746_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1079467.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEK"
     gene            complement(558..1514)
                     /locus_tag="IW746_002593"
     CDS             complement(558..1514)
                     /locus_tag="IW746_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1079468.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1514..2170)
                     /locus_tag="IW746_002594"
     CDS             complement(1514..2170)
                     /locus_tag="IW746_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1079469.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          667 a          485 c          424 g          671 t
ORIGIN      
        1 tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc gataattcag
       61 cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat ccagttcttt
      121 ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag gcacaagtcg
      181 tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga ttgctttatc
      241 actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt gttcagctat
      301 cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt ctaactgacg
      361 ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa aactatagac
      421 atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat tgcccatcgt
      481 tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat ggctgaaatg
      541 caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt tccttggaag
      601 ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt tttcaagctc
      661 cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg gatgatgatc
      721 tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg acggctcatt
      781 aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat cagatgcatc
      841 aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac attttctttc
      901 atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac acggatatta
      961 tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc tcggttgatc
     1021 agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa atacatccgc
     1081 tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg tcgtgtaccg
     1141 actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc tttgattatt
     1201 tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg ttggacatga
     1261 ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag agggtcttca
     1321 ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc gccatgggca
     1381 acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt gaaaattttc
     1441 ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt tccttctgaa
     1501 taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt tttctttttt
     1561 ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtag ccgccgatga
     1621 tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt actataataa
     1681 acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa gctggaagca
     1741 gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta gggaaactgt
     1801 aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa aatagtttga
     1861 tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa aatgtttctg
     1921 cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg atgacggtaa
     1981 gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga taagaagctc
     2041 tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg aactttacga
     2101 tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga aatgatgttt
     2161 tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt tggtccagcg
     2221 aaaaaaacgg taaagagata aaaccaa
//
LOCUS       DACEND010000204         2242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEND010000204 DACEND010000000
VERSION     DACEND010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.214"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..322)
                     /locus_tag="IW746_002595"
     CDS             complement(<1..322)
                     /locus_tag="IW746_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1079470.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQ"
     gene            404..1579
                     /locus_tag="IW746_002596"
     CDS             404..1579
                     /locus_tag="IW746_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1079471.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(1656..2240)
                     /locus_tag="IW746_002597"
     CDS             complement(1656..2240)
                     /locus_tag="IW746_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1079472.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          689 a          427 c          413 g          713 t
ORIGIN      
        1 actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa tacctttttc
       61 tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa ctgatttttt
      121 tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact tttaacaatg
      181 aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt ccttgcgtct
      241 cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt ccttcaatta
      301 tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt atactgtaaa
      361 tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg aatttgataa
      421 acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg agacatatca
      481 aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc ccggtggggt
      541 agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca ctctgtctga
      601 taaactatat caggctatca ttcattggga acgccaacac catgctgtgg agttgacgaa
      661 agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag tacaaacttt
      721 tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat tttcctctat
      781 tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa ataatcattt
      841 catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat
      901 tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat tgaaacagtt
      961 aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc atcaagattt
     1021 aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt tagatgatct
     1081 actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca aaaattcaat
     1141 ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag tgatgaacca
     1201 acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg agacgggtgg
     1261 agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag ttaatcagtt
     1321 ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt acttaatgtg
     1381 gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc ttgttcggaa
     1441 aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg ggcatatgcg
     1501 cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa tcaagtatgc
     1561 tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct tactaggttt
     1621 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     1681 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     1741 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     1801 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     1861 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     1921 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     1981 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2041 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2101 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2161 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     2221 tctagttgtg ggaatgccat at
//
LOCUS       DACEND010000205         2242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEND010000205 DACEND010000000
VERSION     DACEND010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.215"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(773..1063)
                     /locus_tag="IW746_002598"
     CDS             complement(773..1063)
                     /locus_tag="IW746_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079473.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1271..1962)
                     /locus_tag="IW746_002599"
                     /pseudo
     CDS             complement(1271..1962)
                     /locus_tag="IW746_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          640 a          411 c          321 g          870 t
ORIGIN      
        1 cctattagta acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt
       61 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
      121 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
      181 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
      241 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      301 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      361 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      421 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      481 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      541 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      601 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
      661 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
      721 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
      781 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
      841 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
      901 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
      961 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
     1021 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
     1081 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
     1141 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     1201 taaaaaaaag aggcagcagt atagattact gttgcttctt tactaattta ggtttacttg
     1261 ttaagaaaga ttatttacgc aaatgttctt caacaatttg ctgagattta tcagctgtat
     1321 cttccacttc atcttttgct ttttctaact tttgaatata ttgtttcaag cttgagtttt
     1381 cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa ctgatttcta catttgtttt
     1441 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
     1501 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
     1561 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
     1621 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
     1681 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
     1741 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaatcg cttgctcttt
     1801 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
     1861 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
     1921 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
     1981 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
     2041 acatatgtaa ttttaatata aatactatca aatactcatt taatcctcct tttttatcta
     2101 tttaaaaaat cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta
     2161 tggtgaacaa aaacaaaaaa gcaagtttta ttaaacgtaa cccttttcat ttcatagatt
     2221 actgtattta ttacaaaaaa cg
//
LOCUS       DACEND010000206         2218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEND010000206 DACEND010000000
VERSION     DACEND010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.216"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..962)
                     /locus_tag="IW746_002600"
     CDS             complement(15..962)
                     /locus_tag="IW746_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1079474.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IW746_002601"
     CDS             complement(1140..1943)
                     /locus_tag="IW746_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1079475.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..>2218
                     /locus_tag="IW746_002602"
     CDS             2089..>2218
                     /locus_tag="IW746_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1079476.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSL"
BASE COUNT          712 a          363 c          437 g          706 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttg
//
LOCUS       DACEND010000207         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEND010000207 DACEND010000000
VERSION     DACEND010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.217"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..2211
                     /gene="acm"
                     /locus_tag="IW746_002603"
     CDS             46..2211
                     /gene="acm"
                     /locus_tag="IW746_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1079477.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          870 a          348 c          428 g          565 t
ORIGIN      
        1 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
       61 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      121 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      181 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      241 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      301 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      361 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      421 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      481 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      541 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      601 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      661 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      721 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      781 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      841 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      901 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
      961 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1021 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1081 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1141 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1201 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1261 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1321 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1381 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1441 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1501 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1561 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1621 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1681 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1741 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1801 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1861 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1921 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     1981 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2041 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2101 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2161 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta g
//
LOCUS       DACEND010000208         2200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEND010000208 DACEND010000000
VERSION     DACEND010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.218"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(272..841)
                     /locus_tag="IW746_002604"
     CDS             complement(272..841)
                     /locus_tag="IW746_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1079478.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(915..1238)
                     /locus_tag="IW746_002605"
     CDS             complement(915..1238)
                     /locus_tag="IW746_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079479.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1392..>2200)
                     /locus_tag="IW746_002606"
     CDS             complement(1392..>2200)
                     /locus_tag="IW746_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079480.1"
                     /translation="WQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDRHL
                     KRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYEQK
                     YDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQSFS
                     EMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSCNQ
                     ITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          604 a          452 c          387 g          757 t
ORIGIN      
        1 caagtctatg cccatatttc tcccgaagtg aaaaaaagat caatgaatca actagaaaac
       61 tatatagaag agcaaataaa aaagcactca aattgagtgc ttttttaagc tcactatcac
      121 taaaactatc actaatttta tttataagcg aacatgtttc tatcactgat tttttaaatc
      181 tagcgaaaaa cccttatctt aaaggcgttc gttcaattct ttggcaaggt cttcaaagcc
      241 tggcttgcct agaagtgcga acatggtttt cttatagctc atgtagaaaa attttcttga
      301 tgatgatttt atccatcaat tgagattgac tgttttgctc tcctatgatt tcagctatga
      361 ttgtccgatt ttgcggtttt cttcttaact ggtagttgat tttcccagtt tccatcaact
      421 cgctaagcat atccacgact ccgccaaatc cggtcttttt cgtaataaaa cctagtgcta
      481 ccattcgttc atctagattt tttgctgtaa tcgtcacacc agtaatgagc gagtgctgta
      541 atgtttgggc cagctcttcc atcccttcag cattggatac gacggaagct gttttgttaa
      601 taggttcttc gtttatttct tttggttctt tcctctccac tttcactgtt tgacgaagga
      661 catctagttc ttgcaagagc gtttcgtatt gttcttgggt cagcatcacg ccagctactt
      721 tttcacgatt aaatatatat acgccagttc ccgcgtctcg cgcttgagca aatacatcca
      781 tcggcgatcg tttcacttct gtgatcgaag atgtgggaac ttctaatttc tttaattcca
      841 atggatttcc tcctttacta atcgcttttt attatagcac agactgactt gttttgatct
      901 tttttccaaa ggttttatcg gtttttgcct aaccagtaat tgatatcggt aagaagtctt
      961 tcgataccaa atgaaggatg gtacagatac gttttttgat cttcgttatg gtctagtgga
     1021 aaaaaatgat cgatgatcac tagatctgca taccgaggcg gcttatttcg agtacataca
     1081 tgtatagaga tccgttttcg atgtcttagc gttttgttca gtaccccagc aagtcttctt
     1141 gcttcttggt gttcatagcg aaaaaaaaag acgattttca cttcaggagg gtggacatct
     1201 aaataccctc gttcctggag tgttttcagt aaaagcatcc cccactctac tgcggggtgt
     1261 gcaagtgctt gttggatcgc tagccgaaat tgatatagtt tatctttgga attgggaaat
     1321 ttttctaatg tttcctgata ataacgaact tcttctatca gccggtcatg gataccaatt
     1381 acgtaacgaa gtcattcttg gtaaagtact tttgtcagct ttgatttgct ttcgctctca
     1441 aattcgctat tcagccaaaa cagcgaatca agcttttcag tcacaatcgt caatttccga
     1501 tagtcttgtg tctcttctat ccgtttccac cgcttttcag taatctggtt acagctcaag
     1561 cgcaaaattg ctagataatt tttttctttt attggcaaaa tctgctcctc tctaaatagc
     1621 aattcaggaa acaattcaaa gaatagtgtt cgactgtctc ctaaaatctc gatttctgcc
     1681 ggatcctttc tttgcttcaa taattggatg aacatctcag aaaaggactg ctctccatca
     1741 tctgcaaaaa aatgcggaaa atctttcaaa aagcaaaaca gcagatatct tacttttctt
     1801 tctgaaccaa agatctgtgc ttttttataa gaaaaagaaa ggtggtactc gccaaaccac
     1861 tttctcaatc gctcttgcgt agaacgatcg tacttctgct cgtaaggttc attcaatagc
     1921 agatttttca gtaaacaaaa cgggggtgat tggatcagaa aaacaaacaa gagataaggt
     1981 tcgctatatc cttgatcata aactaactga acggttttct tatgaagaat cagctgaatg
     2041 ggacagtcgc tttccgccaa acgtttcaga tgccgatcca ctgttttaac atcgattttc
     2101 agttcttcag ctaaatcagc ttttcgccag atcaaatctt tcattagatt gattagtgaa
     2161 aataaattct gtgcattttt tgataatagt ttttgccatc
//
LOCUS       DACEND010000209         2168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEND010000209 DACEND010000000
VERSION     DACEND010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.219"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..112
                     /locus_tag="IW746_002607"
     CDS             <1..112
                     /locus_tag="IW746_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079481.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            326..1021
                     /gene="vanR"
                     /locus_tag="IW746_002608"
     CDS             326..1021
                     /gene="vanR"
                     /locus_tag="IW746_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1079482.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            999..2153
                     /locus_tag="IW746_002609"
     CDS             999..2153
                     /locus_tag="IW746_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1079483.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          731 a          400 c          461 g          576 t
ORIGIN      
        1 ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac
       61 taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg
      121 tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc
      181 tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta
      241 gtcatttatt atgtaaatgc ttataaattc ggccctataa tctgataaat tattaagggc
      301 aaacttatgt gaaagggtga taactatgag cgataaaata cttattgtgg atgatgaaca
      361 tgaaattgcc gatttggttg aattatactt aaaaaacgag aattatacgg ttttcaaata
      421 ctataccgcc aaagaagcat tggaatgtat agacaagtct gagattgacc ttgccatatt
      481 ggacatcatg cttcccggca caagcggcct tactatctgt caaaaaataa gggacaagca
      541 cacctatccg attatcatgc tgaccgggaa agatacagag gtagataaaa ttacagggtt
      601 aacaatcggc gcggatgatt atataacgaa gccctttcgc ccactggagt taattgctcg
      661 ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa
      721 tgttatcgtc cactccggcc ttgtcattaa tgttaacacc catgagtgtt atctgaacga
      781 gaagcagtta tcccttactc ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa
      841 ggggaatgtg gttagctccg agctgctatt tcatgagata tggggcgacg aatatttcag
      901 caagagcaac aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac
      961 cattgataat ccgaaatata taaaaacggt atggggggtt ggttataaaa ttgaaaaata
     1021 aaaaaaacga ctattccaaa ctagaacgaa aactttacat gtatatcgtt gcaattgttg
     1081 tggtagcaat tgtattcgtg ttgtatattc gttcaatgat ccgagggaaa cttggggatt
     1141 ggatcttaag tattttggaa aacaaatatg acttaaatca cctggacgcg atgaaattat
     1201 atcaatattc catacggaac aatatagata tctttattta tgtggcgatt gtcattagta
     1261 ttcttattct atgtcgcgtc atgctttcaa aattcgcaaa atactttgac gagataaata
     1321 ccggcattga tgtacttatt cagaacgaag ataaacaaat tgagctttct gcggaaatgg
     1381 atgttatgga acaaaagctc aacacattaa aacggactct ggaaaagcga gagcaggatg
     1441 caaagctggc cgaacaaaga aaaaatgacg ttgttatgta cttggcgcac gatattaaaa
     1501 cgccccttac atccattatc ggttatttga gcctgcttga cgaggctcca gacatgccgg
     1561 tagatcaaaa ggcaaagtat gtgcatatca cgttggacaa agcgtatcga ctcgaacagc
     1621 taatcgacga gttttttgag attacacggt ataacctaca aacgataacg ctaacaaaaa
     1681 cgcacataga cctatactat atgctggtgc agatgaccga tgaattttat cctcagcttt
     1741 ccgcacatgg aaaacaggcg gttattcacg cccccgagga tctgaccgtg tccggcgacc
     1801 ctgataaact cgcgagagtc tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg
     1861 ataacagcat cattgacatt accgcgggcc tctccgggga tgtggtgtca atcgaattca
     1921 agaacactgg aagcatccca aaagataagc tagctgccat atttgaaaag ttctataggc
     1981 tggacaatgc tcgttcttcc gatacgggtg gcgcgggact tggattggcg attgcaaaag
     2041 aaattattgt tcagcatgga gggcagattt acgcggaaag caatgataac tatacgacgt
     2101 ttagggtaga gcttccagcg atgccagact tggttgataa aaggaggtcc taagagatgt
     2161 atataatt
//
LOCUS       DACEND010000210         2164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEND010000210 DACEND010000000
VERSION     DACEND010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.220"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            333..1217
                     /locus_tag="IW746_002610"
     CDS             333..1217
                     /locus_tag="IW746_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079484.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1426..2004
                     /gene="pgsA"
                     /locus_tag="IW746_002611"
     CDS             1426..2004
                     /gene="pgsA"
                     /locus_tag="IW746_002611"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1079485.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
BASE COUNT          677 a          392 c          493 g          602 t
ORIGIN      
        1 tttgacctga gtattagaga aacatacgga aaaatatctc cttatatttt gccggttttt
       61 gagctaatac cgaggaacag ctgattgaac accgtttaag aaagaggtcg tgagcctgcc
      121 gtttaactcc gagtcacaag gaacaagttc aaaaaacagc ttcttatatt tattggatat
      181 tgtgacttgt gaccgaggag ttggcaggag aacaatgttt attcggaaaa aaggagctgt
      241 gacatgactt ttgtcacagc acccaaacat aacgtctttc gttgcaacag cagatgcttc
      301 ggagaatcaa acagactgga gaagatgaac gagtggcttt tgaaaccatc ggagaaaaat
      361 tacggcaagc acgcctgaat aaaaaaatat ctttagatga attacagcag attacaaaaa
      421 ttcaaaaacg ttatttggaa gcaatcgaca atgatgattt tgaccaattg cctggtaaat
      481 tttacgtgcg ggcatttatt cggcaatacg ctgaagctgt aggagaagat ggagaccatt
      541 tagtcgatgt ttttgatggg aaaagcaaat tgacaggatc ggtccctgaa cgtccgcagc
      601 ctgaaacagt aagtacttct agaaaagcaa tgcatcagga agaatcgcat ccaagtcgct
      661 tttggacaag tcttcctgtc attttattag gattggttgc tttggcgatt gtcgttgtcg
      721 tgggctatat gacttggcaa gatcgtcaag ctgatccaat aatcggagac acagcttctt
      781 cgcttaaagt agaaggatca gtcgaggagt cctcttcctc agagcaaagc acgcagacca
      841 aagagtcttc tacagtagaa tcaacaacaa aatcaagtga agaaaagaaa atggcaatag
      901 ctacaggaga agattctggc agtgccatcg cagtaaatat cacagacgct accaagcctg
      961 taacccttga atttactgct gtcaatcgtg tatggttagg tgtgctagta gacaatgcct
     1021 atgtttatca agggacactg gcagcaaatg aaacacagag taccgtgtta ccggaaacag
     1081 caacaaatgc gaccatcacg attggtgcag caagcaatgc aacgatcaaa gcgaatggag
     1141 aatccgtacc agttaatcca ggggaaaaca atcagagtcc aaaaaatgtg aaccttactc
     1201 ttcaatacgc agaatgatcg tataaattag tttacgcatc tgtttacttg taggtataaa
     1261 aaagaaggat cgtgacaaat ctgtgtcgcg gtccttcttc gttaattcaa aaagatcaat
     1321 ttcaataaaa atactccttt gaataaattc atttggaaaa gtaaataata tcggtggaaa
     1381 cttcgctgat agtcacgtag aatagggaag aaaggagtcg atagtgtgaa tttaccaaat
     1441 aaattaactg tacttagaat ttgtatgatc ccgattttca ttttggttgt aggtttacct
     1501 ttagattggg gacaatttaa cgtaggagac accacactgg ctgtcacaca gctagtaggt
     1561 gcagttattt ttgctttagc tagtatcaca gactggttag atggaaaaat tgcccgtgcg
     1621 cgaggccttg tcactaattt tggcaagttt gcggacccgt tggcagataa aatgctagtt
     1681 atgactgctt ttatcatgtt ggttgaccaa ggaaaggccc ctggttgggt agtagcgatc
     1741 attgtctgtc gtgaattagc cgtcacaggc ttgcgtttac tacttgtcga acatggagaa
     1801 gtcatggctg cagcttggcc gggaaaagtc aagacggcta cacaaatggt tgctattatc
     1861 ttgctgttta tcaataatat cccattcagc ctgatcggta ttccagtaga tcaaatcatg
     1921 ctttatgctt gtctgatctt tactatctat tcaggtgtgg attacttcat caaaaacagc
     1981 ggtgtgttca aaggatcaat gtaagtatcg tttagtggag aggttgtgag cctgccgtct
     2041 aactctgagt cacaagggat aatttcaaaa aacagctttt catgttttat gtatattgtg
     2101 acttatgacc gaggttttgg caggagaaca ctgtttattt ggaacaagga gctgtgacaa
     2161 gatt
//
LOCUS       DACEND010000211         2111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEND010000211 DACEND010000000
VERSION     DACEND010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.221"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            18..1358
                     /locus_tag="IW746_002612"
     CDS             18..1358
                     /locus_tag="IW746_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1079486.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1576..2019
                     /locus_tag="IW746_002613"
     CDS             1576..2019
                     /locus_tag="IW746_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1079487.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT          583 a          360 c          455 g          713 t
ORIGIN      
        1 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
       61 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
      121 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
      181 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
      241 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
      301 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
      361 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
      421 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
      481 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
      541 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
      601 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
      661 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
      721 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
      781 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
      841 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
      901 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
      961 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
     1021 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
     1081 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
     1141 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
     1201 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
     1261 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
     1321 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
     1381 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
     1441 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
     1501 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
     1561 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
     1621 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
     1681 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
     1741 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
     1801 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
     1861 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
     1921 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
     1981 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
     2041 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
     2101 ccgtgaataa g
//
LOCUS       DACEND010000212         2070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEND010000212 DACEND010000000
VERSION     DACEND010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.223"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1035
                     /locus_tag="IW746_002614"
     CDS             <1..1035
                     /locus_tag="IW746_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1079488.1"
                     /translation="ELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTISNTKDIF
                     RLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIE
                     FNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLLTLHDYT
                     LFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTVIQVPEI
                     VMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRR
                     HPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVVDKTVFE
                     AFQRGLNKCD"
     gene            complement(1124..1516)
                     /gene="rpsI"
                     /locus_tag="IW746_002615"
     CDS             complement(1124..1516)
                     /gene="rpsI"
                     /locus_tag="IW746_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1079489.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1532..1975)
                     /gene="rplM"
                     /locus_tag="IW746_002616"
     CDS             complement(1532..1975)
                     /gene="rplM"
                     /locus_tag="IW746_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1079490.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          684 a          376 c          372 g          638 t
ORIGIN      
        1 gaattaaaag gaaaaataaa atttaaggaa ttctatgaat caaaatggct tcctagatat
       61 gaactaggtc agactactag atcaaaaaga gctccaagtt acgtaacaat tagtaatact
      121 aaagatatat tccgattgca catccttcca atgtttggtg agtatgcaat gaactatcta
      181 aattcaaata ctgagataat atcagatgaa ttaacaaaaa aatcgaaaga atacgcaaat
      241 ataaaaatta tcaaaggata tgtccgttct atgtttgata ttgcagaaat actaaattat
      301 attgaattta accgaactac caaaattatt caatgtatca ctgcacctaa aaaaattgct
      361 ttagaagaaa gaaggatacg ggaaggtaat caagcattat cgtcagaaga gcttattgct
      421 tggatagaag cagtaaacac tgacttgaat aatcacttgc ttactcttca tgactatact
      481 cttttcatgc ttaccctata tcttggtgat agaaaaagtg agacgtatgc tttgcaatgg
      541 aaatatatcg actttgagaa acaaacggtt cgccttaaac atgctttgga taaatatcaa
      601 aaaaagaaat ttacaaaagg tcgaaaggat accgtaattc aagtacccga aattgtaatg
      661 gcattactaa gcaaatggaa atcagtgcaa gctgagcaat tgttgagatt aaaaattaat
      721 caaacaccag atcagtatct ctttacctat actaaaccat ccggagaagt aaattgccct
      781 gttcacgctg attacctaaa ctatcgaatc aattcaatta aaagacgaca tcctgaattg
      841 gctcatttat ctccacataa gcttcgtcat acctatgcaa ctatagctag acaaggcgga
      901 gcaaatatga atcaaatatc tagtgcttta acacactcag atatttctac aacgagaata
      961 tacgtaaata caccagatgt agtagataaa actgtttttg aagcctttca gagaggattg
     1021 aataaatgtg attagtatgt gttcttaaat gtgttcttaa acaagaaaaa gacacttttc
     1081 aaacaattga aaagtgcctt gaaacattga tatataaata atattaacgt ttagagaatt
     1141 gagatgcttt acgagccttt ttgagacctg gttttttacg ttcgaccata cgtgcgtcac
     1201 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
     1261 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
     1321 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
     1381 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
     1441 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
     1501 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
     1561 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
     1621 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
     1681 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
     1741 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
     1801 tgatgacgat gacgaaatca ccagtatcca aatgaggtgt gaaagttggt ttatttttac
     1861 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
     1921 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
     1981 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
     2041 gggcaaacaa taccatttaa catattaccc
//
LOCUS       DACEND010000213         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEND010000213 DACEND010000000
VERSION     DACEND010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.224"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            824..1297
                     /locus_tag="IW746_002617"
     CDS             824..1297
                     /locus_tag="IW746_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1079491.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1306..1533
                     /locus_tag="IW746_002618"
     CDS             1306..1533
                     /locus_tag="IW746_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079492.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          665 a          330 c          312 g          749 t
ORIGIN      
        1 ggtatgtcct atgatgaaaa gattgtacta ttcattgatc ataacgattg gctatttgat
       61 tgtatcaaat ctaggaaata tggtatttgg tatcagtaaa gaattcagtt ggactactac
      121 attatgggaa tctctattct ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa
      181 ataaacatgt gccatctatc tgttggcaca tgttttagga tttatggtac tttttattcg
      241 gcaatccatt ttatttttct acttcttctt aatacctcta acacttttga tttataaact
      301 aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc
      361 gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg
      421 tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa
      481 aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa
      541 cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc
      601 caggcatcta aactagcatt atagaagaga cacacataga atctaaaata actggtttta
      661 aatgtttctt acaatcaaaa aaagctgtta aatgcactcc acaccttcat actctatcat
      721 tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt tacaataatt
      781 attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat ttaaaacatt
      841 gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg gcgtactttt
      901 ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga ccacattatt
      961 tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt tcttattgaa
     1021 actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa acatactaaa
     1081 aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac ttcctaaatt
     1141 tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt
     1201 attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc ttgaacatgc
     1261 gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc aattatcgtt
     1321 aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg
     1381 ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc catccgtttt
     1441 tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt gttgacctat
     1501 aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac agtaaataaa
     1561 aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct attattcaga
     1621 ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg actcatacaa
     1681 agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt ttcctgttac
     1741 gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac tgatagcagt
     1801 ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac cttgaagtgc
     1861 ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt ttagtaagct
     1921 cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa catttactat
     1981 gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta tttttaacat
     2041 tattttgata ataatg
//
LOCUS       DACEND010000214         2046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEND010000214 DACEND010000000
VERSION     DACEND010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.225"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..168
                     /locus_tag="IW746_002619"
     CDS             <1..168
                     /locus_tag="IW746_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1079493.1"
                     /translation="AQFHLFYQVAAPDDYNDLRGQLQLTFQADETAYTVLLGMARLGD
                     QAKVEWRIFDI"
     gene            244..375
                     /locus_tag="IW746_002620"
     CDS             244..375
                     /locus_tag="IW746_002620"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1079494.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            344..580
                     /locus_tag="IW746_002621"
     CDS             344..580
                     /locus_tag="IW746_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1079495.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(658..1629)
                     /locus_tag="IW746_002622"
     CDS             complement(658..1629)
                     /locus_tag="IW746_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1079496.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT          601 a          445 c          419 g          581 t
ORIGIN      
        1 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
       61 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
      121 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
      181 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
      241 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
      301 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
      361 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
      421 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
      481 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
      541 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
      601 ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta
      661 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
      721 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag
      781 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
      841 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
      901 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
      961 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
     1021 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
     1081 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
     1141 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
     1201 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
     1261 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
     1321 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
     1381 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
     1441 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
     1501 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
     1561 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
     1621 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
     1681 ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc
     1741 aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt
     1801 ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac
     1861 tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca
     1921 ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt
     1981 aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga
     2041 ttacct
//
LOCUS       DACEND010000215         2041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEND010000215 DACEND010000000
VERSION     DACEND010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.226"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..1368
                     /locus_tag="IW746_002623"
     CDS             181..1368
                     /locus_tag="IW746_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1079497.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(1460..>2041)
                     /locus_tag="IW746_002624"
     CDS             complement(1460..>2041)
                     /locus_tag="IW746_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079498.1"
                     /translation="QGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTY
                     AGMKVFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFI
                     FLDEPFVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAH
                     GSVESVFNEENLKKAYGDTIFIGETNYIESSKN"
BASE COUNT          610 a          396 c          357 g          678 t
ORIGIN      
        1 ttcccccctt gggccattga acccgcactc gagagactgg ctcctctctc tgataagtgg
       61 attgcgtatg agttagatgt tgaaagtcct tactttactc cgtatctcgt acaaggtagt
      121 gacgcttttc agttgagtgg atttcaatcc aaaattcttt ttttgaaagg tcagatatac
      181 atgtactttt taggcattga tatcggtaaa cgcactcacg ttgcttctat catgaacgag
      241 gaaggaaagg tattactaaa aggtttttca tttcctaaca ccactgaagg agcagagtca
      301 ttaattgagc gaatggtcga ctattccgga gcacccagtg actttgttat agggatggaa
      361 gccacggggc actattggct gtccatcttt tcataccttc acgaatcaga ttatttgatt
      421 cacgttgtga atccccttca aacagacgga tggcgtaaag gtacagaaat acggaaacga
      481 aaaaacgata ttattgattc tgttttgatt gctgacttaa tgcgctatgg ctcatttgtc
      541 gaaacggtct tatccgatga aaatgttttt tctttgaaac aactttctcg ttaccgaact
      601 tatttagtag gtacagcgag cgacttcaaa agaaaaatta tagccgtatt ggatcaagtc
      661 tttccagaat acgcgacgat ttttaccaag cagggcgttt tcggaaaagc ttctaaagag
      721 ttattgctgg cgtttagtgc cccgatcgac ttagaaaaca tttcctcaga aactttggcc
      781 caatacttag ctgagttaag caggaaccga atcaaggcag aaaaagcgga ccaattgaag
      841 caagcagctt ctaactcctt tggagtaaaa tttgctcaag aagcgttcac cttccaacta
      901 cgttcaatga ttgaacaatt aaaattcatt gaaagccaaa taaaggaaac ggaaagagaa
      961 atcaagcaca taatggacac tttaaattct gttattctca caattcctgg aattggtcct
     1021 ataaacggtg cgactattct tggtgagatc ggagatattc aaaaattttc taacccgaaa
     1081 aaactcgttg cttacgctgg gatagacgct tccgtgacac aatcagggca atatgaggca
     1141 acgcataatg ttatgagtaa acgagggtct ccatatttgc gaaaagcctt gttttcggcg
     1201 gctttagtag cttctcaatg tgatcctgtt tttagtgcgt tttaccagaa aaaagtttcc
     1261 gaaggaaaac atcatctaac tgccttaggt gcggtttctc gtaagctttg ttacgtgatt
     1321 catgctattt tgactaaaaa cgagccttat ataattgttt ctgattagct attgaacatt
     1381 caaaaaaact aatggtaaga agcttatttg ttgtaccctt ttttcggaat tatctgttat
     1441 aaaatatttt ttgatttggc taatttttac ttgactctat atagttagtc tctccaataa
     1501 atatagtatc tccataggct ttttttaaat tctcttcatt aaatacagat tctacacttc
     1561 cgtgagcaat tagatttcga ttcaaaagaa taatattatc aaaatacttc tttaccttgc
     1621 ttaaatcatg atgcacaatt agcactgttt ttccctgatg tttcaattct tttaacgtat
     1681 ccataattat tcgttcacta actaagtcta tccctacaaa tggttcatct aaaaatatga
     1741 aatccgcatt ttgagcaaga catcttgcta aaagtacacg ttgaaattgt cctccagata
     1801 attctcctat ttgatgatta gaatattttc ccatatcgac cttttccaaa gcctttgaaa
     1861 ctctttgcca ttctgcattt ttaattcttt gaaaaacttt cattccggca tatgttccca
     1921 tagatacaca ttcttttact tttattggaa aagtaaaatc aatatcagct ttttgctcta
     1981 catatgcaat cttcaccagt tttttattga tatcttcatt attaatggag acctctccct
     2041 g
//
LOCUS       DACEND010000216         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEND010000216 DACEND010000000
VERSION     DACEND010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.227"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW746_002625"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW746_002625"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1079499.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW746_002626"
     CDS             complement(1431..1823)
                     /locus_tag="IW746_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1079500.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gcgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEND010000217         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEND010000217 DACEND010000000
VERSION     DACEND010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.228"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..376
                     /locus_tag="IW746_002627"
     CDS             <1..376
                     /locus_tag="IW746_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1079501.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="IW746_002628"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="IW746_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1079502.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="IW746_002629"
     CDS             1857..>2001
                     /locus_tag="IW746_002629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1079503.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DACEND010000218         1982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEND010000218 DACEND010000000
VERSION     DACEND010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.229"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..914)
                     /locus_tag="IW746_002630"
     CDS             complement(<1..914)
                     /locus_tag="IW746_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1079504.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IW746_002631"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IW746_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1079505.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          655 a          366 c          387 g          574 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 ag
//
LOCUS       DACEND010000219         1929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEND010000219 DACEND010000000
VERSION     DACEND010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.230"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1915
                     /gene="cadA"
                     /locus_tag="IW746_002632"
     CDS             <1..1915
                     /gene="cadA"
                     /locus_tag="IW746_002632"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1079506.1"
                     /translation="KKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIA
                     LLLLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIG
                     EYPEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQ
                     IGQFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFL
                     VQVEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLV
                     VSGAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQ
                     TETVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPL
                     LPTANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSG
                     YLVIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPED
                     KVQRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQ
                     PSKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAV
                     LNAMRSLHVKNKR"
BASE COUNT          625 a          311 c          445 g          548 t
ORIGIN      
        1 aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta acagaaatag ataaggaaaa
       61 agtatctgaa catggaaatg aaaaagagag aaatacgctg ttccggattt tattttccct
      121 tgcaggtatc gctttattgc tattatttga ttttaatgaa ccaattcgct tgatagggta
      181 tttattgatt tatctgctga taggatatga tgtagtgaag aaggcagtga tgaatatcgt
      241 caaaggaaaa atctttgatg agaattttct tatgtctgtt gctacgattg gcgctatgtt
      301 gatcggtgaa tatccagaag ccgtagcagt gatgctattt tatcaaatcg gcgagtattt
      361 ccaaggattg gcagtcagtc attcaagaaa atcgattcgc gaattgatgg cgatccgtcc
      421 agaaacagct cacgtgcaga cgacggaagg attgatgacc gtgaaccctg aagacgtgca
      481 gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta ccgttagatg gaacgatcat
      541 cgaaggagaa tcattagtcg atacgtcagc acttacagga gaatcagtcc ctaaatctgt
      601 atatgttggt gaaacagtat taagtggatt tatcaataaa aataaaccat ttttagttca
      661 agtagaaaaa tcttatgaaa attctactat cagcaaatta ttagaattag tagaaaatgc
      721 cagcagtaaa aaagcaccag ctgaaaattt cattactaaa ttcgcgcgct attacacacc
      781 agtcgttgta ggattggccg ttttattagc tgttttacct ccacttgttg tttctggagc
      841 ggcgttttct gaatggatct atcgagcatt gacattttta gtgatctcat gtccatgtgc
      901 attagtgatc tccgtacccc ttagtttctt tggcggtatc ggaggagcaa gtaaaatcgg
      961 tgtattagta aaaggttcaa attacttgga gttgcttgca caaactgaga cagtggtatt
     1021 cgataaaacc ggcacattaa cgaaaggtga cttttcgatc cagacgatcg atacaaaaat
     1081 cgatccaaag atattcatgc aatatgttgc gagtgcagaa cagttttcta cacatccgat
     1141 tgctcaatct gttttagaag gctacgcagg tccgctttta ccaacagcta atatccaaga
     1201 atttgcaggt gaagggatct tagctgaagt tgatggcaaa caagtattag caggtaatca
     1261 caaattgatg gaaagatttg aaatctcttt tccttcaagt caagaaatcg gtacattgct
     1321 ttatctagcg atcgatcaaa gttacagtgg ttatcttgtt attgcagata ccttgaaaga
     1381 agatgccgta gacgcattgg ttcaattaaa aaaggctggt gtgaagaaca cagtcatgtt
     1441 gacgggcgat tcgaaaaaaa ttgctgatca tatcgggaag caagtaggcg tggacaagat
     1501 atatagcgaa ttgcttccag aagataaagt gcagcggtta gaggaaatct tacaaagaaa
     1561 caataaaaag accgcatttg tcggagatgg aatcaacgat gcaccggtgc tagcaagagc
     1621 agatgtagga atcgcaatgg gtggattggg aagcgatgct gcaattgaag cagcagatgt
     1681 agtcatcatg aatgatcaac catcgaagat tgcagaagca atccatttag caaagaaaac
     1741 attgaaaatc gtcaaacaga acattgtctt tgctattggg ataaaaattt tggtacttgc
     1801 cttaggtgct tttggttttg catcaatggg agatgcagta tttgcagatg ttggggtgac
     1861 agtattagct gttttgaatg ccatgcgaag cttgcatgta aaaaataaac gttgatcaat
     1921 gaagaggac
//
LOCUS       DACEND010000220         1918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEND010000220 DACEND010000000
VERSION     DACEND010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.231"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(189..1550)
                     /locus_tag="IW746_002633"
     CDS             complement(189..1550)
                     /locus_tag="IW746_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1079507.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(1623..>1918)
                     /locus_tag="IW746_002634"
     CDS             complement(1623..>1918)
                     /locus_tag="IW746_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1079508.1"
                     /translation="NRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSIQN
                     ELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
BASE COUNT          636 a          431 c          293 g          558 t
ORIGIN      
        1 tactctctga ataaaacgtg atttccatat ttatagatca ctagtgcgaa aaaaatgacc
       61 aatagattga tgatgatatt ctgagtaaag agtccatata gaaatagcag aagtgtacca
      121 caaagagcga ctgtccattt tattttatct gtctcttctc ttttcattac tttcccacca
      181 tttcaatgct atttttgtag ttcagcataa cgttcactcg gtactttcgc ttttatcata
      241 tatttataaa gtagaacaaa cagtcctaga aaaatcacgc atcctacaat agcgagaata
      301 ttcctattaa atgcctccgc aaataaaacc ctgaactccg gcccttctat ggatgaccat
      361 gtgatcaact gtcctttctt gattccctcg actgctccgc ttttttctgc taaatcactg
      421 agtattggcg caaggtaagt ggccccgtac aaataaatag gcatagtgat gattcccaag
      481 atcagcatac gagccaagtt tcctcctgtt aaaagtaatc cgccgaccgc aatcgaatag
      541 ttgataatcc ctgctaatgg caatacttgg tttcctggca aaataatggc ataaccaata
      601 aacacaggaa caagtaaaat agcagtaacc caaatttctg aacgaccagc taatatcggc
      661 cagtccaatc cgatatatac ttcacgatcc ttaaaccgct ttttcataaa ttctgacata
      721 gcgtctgcta gaggagacaa agattgcatg aacattttag agatcatagg aaacaaactt
      781 aatgctgtag ctgtttggat cgctagatta agagaacctg aaacgccata cgcagcagca
      841 aagccaagcc ccaatccaat aatgaatccc attacagagt tttcagagaa gatcccaatt
      901 ttcttcttca atgcattcgt atccgctcgt ttatttaaag caggaatttt atccatgatc
      961 atattgaaag gcagcaacaa gacagcggca gatgtcataa aatgagtcac tgttactaat
     1021 ggaatccctg tgagatcttg tatatgtttt tgaaacatat caccaagctt taattccaat
     1081 ataacttgga ccattgcagt aagaaagcct gcagccaaac taccggaaac ataataaact
     1141 aaataagcag ttaaagcttt cccccaaaca ttccacatat caacatttaa cgtcttcgtc
     1201 caattaaaaa gtaacagtac aaaattgacc ccaatcgtta ctgcaaaaaa tataaaagca
     1261 taactccacg accatgtgat gcttgctgca cctgtccacc caagatcaag tgcaggcaaa
     1321 ctgattcccg tatttttagc caatgcttct gatgctggac ttactgcgtt agacatatag
     1381 ccaatgacca tagacattcc gctaaaagca atccctaaag tcaaagcaga catgaatgct
     1441 ttttttaatt tcatccctgc caacaatcca agtatcaaaa taatcagggg aacaaaaatg
     1501 gctgatccta agttcaaaac ataattgata atggtttgga aaacttccat aaataaacct
     1561 tctttctttc taaatatagt aaataaaact atgaaccaac ttttttatcc aacaatcctc
     1621 ctttactgta ctaactcttt gatactgtca taaataggtt ctacacccat tccagttaaa
     1681 aatggaattc ctggaaatac ttcaataccc aaatcttttg cttgttcaag tacttcctca
     1741 tcaggttgtg cgacatacac atagattcca gcagttagaa gctcattttg aatcgatttg
     1801 taatcaactg cttctacagg aaatgcgatg ccgtcatctt ctaacatact ttggatctta
     1861 ctagcgattg tctgactcgt tgctactcca cttccacatg cgacaattaa ccgatttt
//
LOCUS       DACEND010000221         1911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEND010000221 DACEND010000000
VERSION     DACEND010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.232"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..133
                     /locus_tag="IW746_002635"
     CDS             <1..133
                     /locus_tag="IW746_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1079509.1"
                     /translation="EKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            184..1809
                     /gene="groL"
                     /locus_tag="IW746_002636"
     CDS             184..1809
                     /gene="groL"
                     /locus_tag="IW746_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1079510.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT          681 a          323 c          437 g          470 t
ORIGIN      
        1 tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg
       61 tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc
      121 gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca
      181 atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta
      241 gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta
      301 gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc
      361 gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa
      421 acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt
      481 cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc
      541 gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca
      601 aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg
      661 attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa
      721 gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca
      781 caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg
      841 atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg
      901 caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact
      961 cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc
     1021 ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt
     1081 actgacgatc taggtttaga attgaaagat gtaacaatcg aaaacttagg aaatgcttct
     1141 aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca
     1201 atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac
     1261 cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt
     1321 ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat
     1381 gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac
     1441 gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc
     1501 gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga
     1561 tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact
     1621 ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct
     1681 gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca
     1741 gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggt
     1801 atgatgtaag aaaaagcaac ttgcttatct acaactgatc attagtgaaa gacagataaa
     1861 aagaggctgg aacagaagcg tttaactcca agaaataaga agaaattcac g
//
LOCUS       DACEND010000222         1905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEND010000222 DACEND010000000
VERSION     DACEND010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.233"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..722
                     /locus_tag="IW746_002637"
     CDS             126..722
                     /locus_tag="IW746_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1079511.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            735..1634
                     /locus_tag="IW746_002638"
     CDS             735..1634
                     /locus_tag="IW746_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1079512.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1637..1905
                     /gene="ssb"
                     /locus_tag="IW746_002639"
                     /pseudo
     CDS             1637..1905
                     /gene="ssb"
                     /locus_tag="IW746_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734556.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          703 a          300 c          398 g          504 t
ORIGIN      
        1 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
       61 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
      121 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
      181 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
      241 gggagctgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
      301 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
      361 tgtatgattt aacagatatg atccctagac agatcgatct gactgtacct tacggttatc
      421 acgtttctga aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat
      481 ggtatgaatt aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg
      541 atccagaaag aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca
      601 aagtaaaagc aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg
      661 agtatcgacg aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt
      721 aaaggagaag atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag
      781 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
      841 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttgattggct
      901 caaagtatgg cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga
      961 aagtaaccaa agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag
     1021 aagacattca atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag
     1081 gatttagctt acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     1141 tgactacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     1201 aggatcgaac gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa
     1261 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     1321 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     1381 ttacacgtcg aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac
     1441 tgaaaaatag tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     1501 ctgcgtcgat cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga
     1561 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     1621 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     1681 tgatttacgt tataccgcaa gtggtactgc agtagcgact tttaccctag ccgtcaaccg
     1741 aaatttcacg aatcaaaacg gcaattgaga agccgatttt attaactgcg tgatttggcg
     1801 caaaccagca gaaacaatgg cgacattagc taaaaaaggt agcttgattg gtgtagttgg
     1861 gcggattcaa acgcggactt acgacaacca gcaaggacaa cgtgt
//
LOCUS       DACEND010000223         1898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEND010000223 DACEND010000000
VERSION     DACEND010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.234"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..269)
                     /gene="ssb"
                     /locus_tag="IW746_002640"
     CDS             complement(<1..269)
                     /gene="ssb"
                     /locus_tag="IW746_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1079513.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRV"
     gene            complement(272..1171)
                     /locus_tag="IW746_002641"
     CDS             complement(272..1171)
                     /locus_tag="IW746_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1079514.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            complement(1187..1780)
                     /locus_tag="IW746_002642"
     CDS             complement(1187..1780)
                     /locus_tag="IW746_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1079515.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
BASE COUNT          521 a          369 c          284 g          724 t
ORIGIN      
        1 acacgttgtc cttgctggtt gtcataagtc cgtgtttgaa tccgtccgac aacaccgatc
       61 aagctgcctt ttttagctaa tgtcgccatc gtttctgctg gtttgcgcca aatcacgcaa
      121 ttgataaaat cagcttctcg attgccgttt tgattcgtga aatttcggtt aacggctagg
      181 gtaaaagtcg ctactgcagt accacttgca gtataacgta aatctggatc tttggttaat
      241 cgtcctacta aagttacatt attaatcatt tttatctctc catttcttga ttcatttgtt
      301 gtgatctctt aaaattttgt tttcgttcta ctttctcttg ctctacaatt gattgcatca
      361 aagaatcgac ggaagttagt gtagattcga aagaaatgtc tttggcaaaa taattttctt
      421 gttggtatct tttccacttt tccctcaacc cctcatcctt catcaattga ggcatttcct
      481 gattatacaa ttttttaaac acagattgat tatttctttc cttgaacgtt ccttgaacac
      541 tcttatacaa gtttttctca tcgatacgtt ctagttttgg aatcatatac aggtcataaa
      601 agtctttcat gcgcgaatta tcttttccga aactaaacgt agcatgtaat ttttcagcta
      661 gaatttgttc cgtaggataa gctggaatat gaatcgtttt atcttcaaac attaatttgt
      721 gttcatacat caatgccgct ggaataatag aatcacctga agtaatatct aatttaaatg
      781 gtacacgtgc attctcaaga taagcaataa actttgcttg aaaacctggg taataatcgg
      841 cttccctcgt ctctgttaat ccctttaatt caaatcgaat tccatctttt gtgggatcag
      901 ctgttaactc agaaaaaaca gtttctaact tttcttttgt tagtttcgaa tttcgataag
      961 tcgtatcaat atcaacagtt gaacggtttt ctaacccgta ttttgatccg atcagaaatc
     1021 ccccttttaa tacaaaatca tcatgataag gcgattcaac aatacgttct aataattttt
     1081 ccatcatata atatctttgg acaatttgtg gatttaaatg tttctctgtt gcgaaatttc
     1141 tcgtttttgc tttaaattgt gtcggtgtca ttcaccttta ctcctctcaa taaagtggca
     1201 acatatacaa agtcatttct tttggtgatt ttaaaatatc catgtatctt cttagcttat
     1261 ttgtatttct actggtactt gtcatatatt cttttaatgc ttcttgtttt acctctacgc
     1321 ttgctttgta tcttggattc cacatatcac ataacgttcg ctctaaatca tagactaaaa
     1381 ttttattgcc gtaaaagctt tctgtttctg tgattcccag gtcaaaattt tctttattcg
     1441 tgtaatgcgg ttgaatagca tactcgtcta atttctcctt tgaaacattg tatcctttag
     1501 gaaacatcat attgatcttt ttagggatat acgttccgac ttgaaaaaga tcgagcgcag
     1561 tctcatgaga aaaaatacct ctggaataga tactttgagc gagatacaat tcatccacga
     1621 acgcttcttt taaggtatag aaacctggtg ctaccttttc tattttgtcc ttttcggcta
     1681 atcgatctaa cgtcataggc gataagcctt cacttctagc atccttcaag gatagattcc
     1741 cattatgttt atcaaaaatg cactggattt tgtctttcat tgcttttccc ctaaagttaa
     1801 tataaaacta acataactat atcacgttag ttttatatta acaagaaaga tatttatcga
     1861 tctaattcaa tttctttttg aatccgttgc tggttctt
//
LOCUS       DACEND010000224         1886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEND010000224 DACEND010000000
VERSION     DACEND010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.235"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..157
                     /locus_tag="IW746_002643"
     CDS             <1..157
                     /locus_tag="IW746_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1079516.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            652..1251
                     /locus_tag="IW746_002644"
     CDS             652..1251
                     /locus_tag="IW746_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1079517.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1315..>1886
                     /locus_tag="IW746_002645"
     CDS             1315..>1886
                     /locus_tag="IW746_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079518.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          676 a          296 c          363 g          551 t
ORIGIN      
        1 ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct cgtcttttga
       61 tacttacaac caaaaattcc taggtcgcag tcataaaggt ttccaacagg ccgaaggcga
      121 gctcgaacaa atgctgagtc aaccgatgga gaattagaaa caatctacag tagggaagaa
      181 ccattgacac aaaattattg acgctcccaa ctaaaagtgt caaaataatg atgaaatcat
      241 ttagttctta taaattagaa aagagaaaat aaatattcca tatctacttt attggatccg
      301 taacatgact aaattctgaa tactttttaa agcttatcat ttggtaaaac tgaatataag
      361 ttaattatat agactgaaaa ttttgaattt tcagaataga ataacttaaa agtactgaca
      421 aaatttttta aaggggcaat attaatgttc tgagataatc aaatcctacc gaaaatatca
      481 aaaatccaat gtgacacaat cagattgtgt ctcattcgag ggaatcacac aatcccacgt
      541 ggctctaggc agaattgaat ttttgcttgg ggctttttcc ttatattgat tttgaatgag
      601 acttattata tgttataatg caattaagta tcattggaga gggcgaagaa aatgaagacg
      661 gtagaaccta ttcgagacaa aaaaaagatc gatgcgatga aggcaatctt ggcttcggga
      721 aaatatggcc aacgaaattt ggttcttttt tcgattggga tcaacaccgc ctatcgcatt
      781 tctgatttgc gtcaattgaa gctggccgac gtgctggaaa tttcacgcgg tcgcgtggtg
      841 gtcaaagatc gattagcgat gaaggaacaa aaaacagcta aacataattc ggtctatatt
      901 tccaacaaat tgcgaaaaac gattttggag tacgttcaac aggaatttcc tgatcaactc
      961 cttcagcaag attttgacgg ttatttgttc cctagtcgaa aagggacgaa tgaaccgctc
     1021 aaccgccaaa gtctgtggcg aatcattcat gatgctggca cggcaatcgg attgaaagac
     1081 attggcccac attccatgcg aaaaacgttc ggttactttc tctataaaca ggggaccaaa
     1141 acagaaatca ttcagtcctt gttgaaccat tcttctcagc gcgaaacatt gcggtatatt
     1201 ggtattacac aagaagacaa agatacagct gtaaaaagct tagatttata atataaaagt
     1261 taagtttaaa catggatagt tttaaattag tagattgagt gttgggagga aagaatgagt
     1321 acattatata ttataggaaa cggttttgat ttaagtcatg gtttaaaaac tagatacact
     1381 gatttttcaa aatgggttga aaaaagtcat agtgaaatat tcgataaaat agacacaatg
     1441 ataaatgaaa ataatgaggt gagaagtaca gctgatgata agataggttg gtttaatttt
     1501 gaaagtagtt tgcatgagtt aattcaaagt ccagtctttg aagattatgt agaagagtct
     1561 aaggattatc tcataggtta tggacatgaa gattggtcag attctgccca tcatgatttt
     1621 caaaatatgg tttatgatta tgttgtttct ctagaagaat tacaacaact tttcaaggaa
     1681 tggatacgca aagaaataca gccgacaata gaacataaaa ttccaaaata ccaattttcg
     1741 tccgatgtac tattctttac attcaattat acagatgttt tggaaattct atacaagcaa
     1801 ccgaaagaaa acatatatca tattcatggc agttatgacg atataataat cggccactcc
     1861 gagaattttg acaactttat aggaga
//
LOCUS       DACEND010000225         1875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEND010000225 DACEND010000000
VERSION     DACEND010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.236"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1305
                     /locus_tag="IW746_002646"
     CDS             79..1305
                     /locus_tag="IW746_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1079519.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1413..>1875)
                     /locus_tag="IW746_002647"
     CDS             complement(1413..>1875)
                     /locus_tag="IW746_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1079520.1"
                     /translation="IKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAPPSW
                     IFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFI
                     AAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          701 a          293 c          377 g          504 t
ORIGIN      
        1 ttatgattaa aaagtgaatt tgctgattga acagttatat gagacaatat tttagaaaat
       61 gtgaaacaga ggtgtattat gaagcgatgg caaaaagttc tattaacctt gttaggggta
      121 ttcgttttgc tgattgccgg attatcagca tacggaatca gatttatggg agaagcgagc
      181 caaacagtaa acaaaatttc taaaaactct aatcgaatat cctccaaacg ttctgagaga
      241 gtaagcattg atgacaaaga acctttttcg gttttgctat taggtttaga taccggtgga
      301 ttaggaagaa cggaacaagg acgttctgat acgatgatgg tagtaacagt taatccgaaa
      361 caaaaaaaat caacgatcat cagtttggac cgagatattt atacgaatat cgtaggttat
      421 ggaacggtag ataaattaaa tcatgcctac gcatttggtg gtgtcgaaat ggccatggat
      481 tcaattgagc aactgctcga tattcctatt gatcattatg taacgatcaa tttggatggc
      541 atggaagatt tgatcaatgc ggtcggtggc atcaaagtga ataataaaat tgactttact
      601 ttggatggtg tccatgtacc agtaggtgaa caaacattga atggagaaaa aggtttagcc
      661 tattctcgaa tgcgacacga agatccagaa ggcgatatcg gtcgacaacg tcgtcagcgg
      721 gaagtagtga cgaaaatcgt caataaagtt ttaagtttag atggcgtaag caattataga
      781 aaaattttaa aagcagttga gaacaatgtg acgacggact tagattggga tgacatgctt
      841 gatgtagcga cgaactacac cccggcgttt gaaacaatca aacaagacca gttgcaaggc
      901 gaaggacaaa tgatcaatga tatctactat caaattttag gaaaaaatga tcttctttcg
      961 atccaaaatg aactgaagaa acaattaaat ataaaaacaa gcgatacatt acctaatttg
     1021 aaaaatgaga atgcttcaat gatgttcttt gatgattcgg aagatggcga ttcgacaaac
     1081 ggtgatggat cgaatagtga agggtacacc gattattctt cctattcaga aaacacatat
     1141 gatcagtacc aagatccgaa tacctattat cagcaaacag aagatacaag tcagtactat
     1201 acaccaagtc aagatactca aagttatgat ccttacgctg gacaggaaca aaatggttat
     1261 ggttatgacc agaataatga gtatggaaca acaagtgggt actagaaaaa aatagtttgg
     1321 ttaataaaga gtgccaccta aattagacaa aaaatctaac ttaggtggca ctttttctat
     1381 gtgttaaatt acaggacgtc tctttttagc gcttagttta aaaaataaat tgaataattc
     1441 aatagagttg cgaatagtac ccataaataa taaggaatca tgagccacat tgccaggcga
     1501 tgatgcagcc acaactgata cataagcact gacaagaaaa taactaaaag tgtaatatcg
     1561 attgctgcaa tgaaaatatt tcttaaatta aaaaatagag cagaccatat aaaattaaaa
     1621 atgaactgaa tcacaaaaag accgacaaga ttgtttcgct gttttttgtt gttgtgatta
     1681 agtaacaaat aaagagaaat acccatcaat atatacagta aggtccacat cggcccaaat
     1741 atccagctag gtggtgcaaa tggtggaagt tgcaaagaat agtaatattt tccgggattt
     1801 ccgctaagca accctgataa aaagcctaat atgacaattc ctataacaca aacccaaaaa
     1861 cgaatatctt ttatc
//
LOCUS       DACEND010000226         1817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEND010000226 DACEND010000000
VERSION     DACEND010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.237"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..370
                     /locus_tag="IW746_002648"
     CDS             2..370
                     /locus_tag="IW746_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1079521.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(480..1775)
                     /locus_tag="IW746_002649"
     CDS             complement(480..1775)
                     /locus_tag="IW746_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1079522.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          556 a          327 c          370 g          564 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctggctct ttgtcaaatg atgttggtaa
      421 agagaaatct tagaataatt tggacagaac agaaagaaga aaacctcttt ctgttctgtt
      481 tatttggtta aaaatgtttt gccatgacga atcataattc gtagtttgaa attgtaaaag
      541 ttgcgaaagc cgtacgcatt gcgttttaat actttgatgt ggttgtttaa gcattccaat
      601 ggtccgtttg aatagctgta gtgaagcgca ttttggatct gtggcagaaa ctttttaaac
      661 gttcgtaaag tggttgtata ggtttctggc agaccgctaa tgtctctagt taaacaatcc
      721 tcaaatagtg actgatctct tttctttacc gcataaagaa tgtcttggta taactgatag
      781 ccttggcgta agttgggaca agcagctaac agtcgatcgg caatctcact ttcagtcaaa
      841 taatctttaa aactggcacg ccaaacccgt ttttcatagt ttaactttgc ttcttctttt
      901 tgtaggagtt tccaatagcg cttcaatttt ttccctaacc ctcgttgaga gtgggaattt
      961 cctttaagtc gtaccgtggt ttctttcact cgatgattca agaaagttct gccaatatgt
     1021 tggacgatgt ggaaacgatc gatgatcaac tgagcattcg ggaaaagctt tttcaccaac
     1081 gaaacataag gcgcatacat atcaataaca atcaattgaa cggcttcacg agtcgctaat
     1141 gaaaaccgag aaaaatagcg ttctaaataa ggcaagcgtc gattttcgac aatatccaat
     1201 aatgcatggc tttgtccgtc catcatgata aaactcattg cactggcaac acttcgaacc
     1261 gatttgaatt catcaaagca taagaccttt ggcaaagtct gtttgaaagg attcaatggg
     1321 cggtagaatc gcttcatgac acgatagacc gaagaagtgg aaatattttt ctgacgagcg
     1381 atttccgtca tggaagtatt ctcttttaag cgagcaatga cagaccaacg aactttttga
     1441 ctaatgaaac agtgtttttc agcaacagag gtatccgcta cgaatgttct ttggcaagac
     1501 ttacatttaa atcgttgttt cttcagctcc agataagtgc gatactcaga cacatcattc
     1561 agtaacaacc gaacggttgt cgtcccccaa cgaatgatcc tagaggggac gacacaaaga
     1621 caatgggaac actcttctgg cttgtacaga agagttcctt tgtaaatcaa gcaaatttgc
     1681 cctttgtatt tttctttagt taaacaattt tcctcgaaat agatatttag gtctagaata
     1741 tttagggtat ctttgataag ctgagtatga gacatcttga atcctcctat gaattggtag
     1801 ccaaattaat tctaaag
//
LOCUS       DACEND010000227         1802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEND010000227 DACEND010000000
VERSION     DACEND010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.238"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..1237
                     /locus_tag="IW746_002650"
     CDS             77..1237
                     /locus_tag="IW746_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1079523.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1253..1696
                     /locus_tag="IW746_002651"
     CDS             1253..1696
                     /locus_tag="IW746_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1079524.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          629 a          286 c          363 g          524 t
ORIGIN      
        1 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
       61 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
      121 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
      181 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
      241 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
      301 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
      361 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
      421 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
      481 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
      541 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
      601 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
      661 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
      721 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
      781 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
      841 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
      901 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
      961 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
     1021 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
     1081 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
     1141 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
     1201 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
     1261 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
     1321 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
     1381 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
     1441 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
     1501 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
     1561 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
     1621 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
     1681 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
     1741 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
     1801 ct
//
LOCUS       DACEND010000228         1784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEND010000228 DACEND010000000
VERSION     DACEND010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.239"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..621
                     /locus_tag="IW746_002652"
     CDS             <1..621
                     /locus_tag="IW746_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1079525.1"
                     /translation="TKIPAESELIESYEVSRITVRKAVEELVEEGYLLKKQGKGTFVN
                     RHKVFRKIEYVMGFSESCKSNGFTPTSIVLERKIIPATEGMAKKLDIAQGDDVLYIQR
                     KRMADNIPIMLENNYFSYERYSFLMEEELTDSLYEVLKVQGTLAINPGETALEIVTAD
                     DQIAKIMEVAVGTPFFFMDTLINDQNNIPIHVGNQYYLGEVYRFYL"
     gene            657..>1784
                     /locus_tag="IW746_002653"
     CDS             657..>1784
                     /locus_tag="IW746_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1079526.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT          610 a          271 c          390 g          513 t
ORIGIN      
        1 acgaaaattc ccgctgaatc agagctgata gaaagttatg aggtcagccg aatcacagtg
       61 agaaaagcag tagaagaact ggtagaagaa gggtacctac ttaaaaaaca agggaaagga
      121 acttttgtca accggcataa agtttttcgt aaaattgaat atgttatggg tttcagtgag
      181 agttgtaaat ctaatggttt tacaccgact agcattgttt tggaacgcaa gatcattcca
      241 gcaactgaag ggatggcaaa aaaactcgat atcgctcaag gtgatgatgt tttatatatc
      301 cagagaaagc gaatggcgga taacatcccc attatgttag aaaacaatta tttttcttat
      361 gaaagatata gtttcttaat ggaggaagag ctgactgatt ctttgtatga agtacttaaa
      421 gtccaaggta ccttagccat caatcctggg gaaactgcac ttgaaattgt cacagctgat
      481 gaccaaattg ccaaaataat ggaagtagct gttgggacgc cctttttctt tatggatact
      541 ttaatcaacg atcaaaacaa cataccaatt catgtaggta atcaatatta cctgggagaa
      601 gtatatcgtt tttacttatg atttcctgtt ttgatttcaa ggagtagatt ttatttatga
      661 agcaaatatt gcggtcaaaa atgatttttg atggagagca gccacctttc aaaggttata
      721 ttgtaatcaa tgaagggatc attgaaaagg tggaaaacaa ctggaattac agtgatctat
      781 taaatgaaaa cacggaatta attgcttatg atgatcaatt cgttatgcca gcaattcatg
      841 ataatcatgt atttttcagt ggttatttgg ctatgaatgc tggcatagat ttaagcgata
      901 catcttcagc taacgaggct gtagagcgta ttaaagctgt aatgaaccat aaaaaagcag
      961 atgaaccaat ttatgctcat ggctgggatg ctgccctgtg gaaaactgac ccatcagaag
     1021 agctattgaa tgaaataaac tactttggtc caatcacagc gatcgacaaa aatcgcagcc
     1081 attgttggat gaatgctatt gctaagcaga cttatggttt tacagaaaaa gaaaccagtg
     1141 ctgaaagtcg agtattatta attaaggaaa tgctagcaaa caaaaagctt ttagcagagg
     1201 tttacgctga atttgaagca ttactgcttt caaaaggtgt tatatcaatg aaagaaatcg
     1261 tttttgatga cgcagaattt ctttctaata tacctcagag gaaaatactt agtaacctat
     1321 atgtgcaggc agtccaagaa cctctacagc atgaacttct atatgagtat aaggaaaaag
     1381 ttttccctga aaaggtccgc tttggtggag tgaagatcat ggtagatggt gttgttgcgg
     1441 atagaagtgg agatatttac ggtgtttatc agtcaggagt agcttcaccc gagattgact
     1501 atgaggcaat taaagcagaa gttgctgcat tcaatgaagt agagattcct tgctgtttga
     1561 caacggaagg agataaagcg ggttataaag ctgcgtcaat tcttgtcgaa tttggaaagc
     1621 ggttacctga aggcgttttt aattcgatta gtgatttgga aatggttact caaaagacag
     1681 cagaaaaaat gaacgaagga caagtagtag cagaggtata tccacaaatt ctcggcttga
     1741 atcaaacgtt agaagaggct tacatgtcaa ctgttataaa aaat
//
LOCUS       DACEND010000229         1773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEND010000229 DACEND010000000
VERSION     DACEND010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.240"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1444)
                     /locus_tag="IW746_002654"
     CDS             complement(<1..1444)
                     /locus_tag="IW746_002654"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1079527.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          495 a          338 c          327 g          613 t
ORIGIN      
        1 cttcttcttc aaaggtatcc acgtaacggg gaatgtttaa gttgtaatca ttctccacaa
       61 tttcatcaaa ttctgcgaca taagcatact tgtctttaat tgtacgggtt ttataggcct
      121 ctaaaatagc gtcaatatgg taatctcgta gcgtattttg cgtttttatt ttttcaaaat
      181 cttgtgacgc atcaataaag agcacatctt ttttcgcacg gtctttcttc aagataatta
      241 cggtcgttgg aatggacgta ttaaagaaga tgttagcagg taagccaatg acggtgtcaa
      301 tggcaccatt ttctaacaag gctttgcgta tcttcccttc tgcgccccct ctaaagagca
      361 ccccatgtgg caaaacaatg gccatgacgc ccgtatcttt caaatgatag tagccatgta
      421 ataagaaagc aaagtccgct tttgatttag gggctaatac gccataggca gagaagcgtg
      481 ggtcatctaa gaagccttta tcagccgacc attttgcaga atacggtggg ttcatgagca
      541 ccccatcgaa gttcgttggc tcttctgttg gccagtcttg atccaaggta tccgcattat
      601 gcaagtgttg gttggcaatg ggaaccccat gcaaaatcat gttcatacgc gccaaattat
      661 aggtagacgt attcaactct tgtccgaaat aagaaatggt tcccggttga tgggaatatt
      721 ttttagcgtt caaaagtaac gagcccgaac ccatggtcgc gtcgtaaaca gaaaagcctt
      781 ttttatcttc ttttccttgt aaaacaattt gtgtcatcaa ttttgcgacc ggttgtgggg
      841 tatagaattc accggctttt ttaccggatt cagatgcgaa ttggccaatt aagtactcat
      901 aggcatcgcc taagacatcg ccagcatgcc ccgccatgtt taacccttct aattctttca
      961 taacatctgc gatggtttta ttttgttttt gtggggttga ccccagtttt ttagaataca
     1021 gatcaatgtc ttcaaataag ttctcaaagt tttcgctgga ttgttcaatg tcacggaaac
     1081 cttgtaccag gtcttctaat tgaaaagatc catcatgaat ggctgccact tgcgcggtaa
     1141 aggtcaatgc gggtgcgatg gtatatgaaa actcatcttt tagttcatac aagagatcat
     1201 cctttatatc tgcatcttta aatgcttcaa cgtaaaggtc ttgtgcttta tttaggtcgt
     1261 ccgtttcttc ctccaataga acggagacat accgcagcat gttgtcggat aagtatttat
     1321 aaaagaccaa gcctagtaaa tagctcttgt attcattagc gtccattttt gaacgcagga
     1381 tatctgcgga attccacagt gcctgatata agctttttga atttttttcg atttgttctg
     1441 tcattggtta ttttcctcat tcgtgttgta ttttttataa atcagttttt taaaaaatta
     1501 gttactattt gaacttgttt tttcaccata tgtattttaa tgaaatggat aagctacatt
     1561 tgcttagaca attcatgtct tacttaattc ttattataac aaacctattt cacctttttc
     1621 gatacattaa ataataagta acttaaaata aggttcgaat aaccttgtta aatagtggtt
     1681 ttcttatctt atagtataga aatcaagaga ttacttccga tataacattt ttttaaaaaa
     1741 attagattgt aatgtttttt atattcattc taa
//
LOCUS       DACEND010000230         1748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEND010000230 DACEND010000000
VERSION     DACEND010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.241"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1417
                     /locus_tag="IW746_002655"
     CDS             <1..1417
                     /locus_tag="IW746_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1079528.1"
                     /translation="KGFWIIGEERLVRLTIEEILQKEFTDYDIYKLMSLLLNGGETEY
                     FEMYSATATPIQEVFNQVIIRMRHLLEFENLEKLNYAELLNILIRVTIATVRLRKEAT
                     IGRYQLVAEQELDKNDLSYRVMQQVFDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLV
                     ENVIYKVSKAINHSFYQDPQLLTNLYAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELF
                     DAIKQAVRQEISEQNILINDSFVAYIALHFLVSYEREEDLRSVRAVYICSTGLGVTSL
                     IKQKINEELNNITIVAFASVLNAQEMIATKDPDLVISIFPINGIDRPFIKVHPLPTEQ
                     DLKKIQQIIKKILASSNQQERHNLNLRETSINKDTLEDFSKELILKAYTIYERLLVIL
                     EAELIQEYREAFLLHVFLMVHRITFDQQYMAEGRQVDQIILDKGTLVGKIEAVFAENN
                     LGINQSEISALFSYIKEGASS"
     gene            1417..>1748
                     /locus_tag="IW746_002656"
     CDS             1417..>1748
                     /locus_tag="IW746_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1079529.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          622 a          272 c          331 g          523 t
ORIGIN      
        1 aaagggtttt tggatcatag gagaagaacg tctagttcga ttaacgattg aagagattct
       61 tcaaaaagag ttcacggatt atgacattta taaattaatg tcattgttat tgaatggcgg
      121 tgagactgaa tactttgaaa tgtattctgc tactgctaca cctatccaag aggtatttaa
      181 tcaagtaatc attcgaatgc gacacttact ggaatttgaa aatctagaga aactgaatta
      241 cgcggaatta ctaaatattc tgattcgagt gacaattgct accgttcgct tacgcaaaga
      301 ggctacgatt ggtcgttatc aattggttgc tgaacaagaa ttagacaaaa acgatctttc
      361 ttatcgcgtc atgcagcagg tctttgatta ttatcagttg ccactgttcg aggatgaata
      421 tcattatatc tatagtgata cattcgacac gattcaccag caagacgtca tgaaactagt
      481 tgagaatgtg atttataaag taagtaaagc aatcaaccat tctttttatc aagatccaca
      541 attgttgacg aatctgtatg ctcatttatc catgcgatta tcgagaaaga aaaaatttgt
      601 caatgaatat aatcctttta aagaagatat caagcagaaa tacccggaat tatttgatgc
      661 gatcaagcaa gcagttcgac aagaaataag tgaacaaaat attctgatca atgattcttt
      721 cgtggcatat attgccttac actttttggt ttcctacgaa agagaagaag atctacgaag
      781 cgttagagcc gtctacattt gttcaacagg gttgggtgtg actagtctga tcaagcaaaa
      841 gatcaatgaa gaattaaata atatcactat cgtggcattt gcttcagtat tgaatgcgca
      901 agaaatgatt gctaccaaag atcctgattt ggtgattagt atttttccaa ttaatggcat
      961 cgatcgtcct ttcatcaaag tccatccatt acccactgaa caggacttga aaaaaattca
     1021 acaaataata aaaaaaatac ttgcttcttc caatcagcag gaaaggcata acttgaatct
     1081 tagagaaacg tccatcaata aagatacttt agaagacttt agcaaggaat tgattttgaa
     1141 agcttatacg atttatgaac gtttacttgt cattttggaa gcagaattga tccaagaata
     1201 tcgagaagct tttttgcttc atgtattctt aatggttcat cgtatcacat ttgatcaaca
     1261 atatatggca gaaggtcgtc aagtagacca gattatttta gacaaaggaa cactggtagg
     1321 aaaaatcgaa gcagtttttg cagaaaataa tttaggaatc aatcagtcag aaatcagtgc
     1381 attattctca tatataaaag aaggagcgtc aagttagtga aaatgtcaga cgaggcttta
     1441 aaaattatcg aggaaagtca aaatcgggaa aagttaaaac aagtgattga tctaattgaa
     1501 gagcgattag tagaacatgc tattgtacca accgagttgc agtggaccat tctaattaat
     1561 cacttgaatg agatgctgaa acgttctcaa agaaaggaaa cgattcccgt agtcgatcgg
     1621 gatttgttca aagaagtatc caaagaagcc ataactattt ctgatgaaat agttagaagt
     1681 atcggtaatt taaccgatga tgaaatatat gttttgtcga ttcattttga aacagcaaaa
     1741 aataatta
//
LOCUS       DACEND010000231         1746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEND010000231 DACEND010000000
VERSION     DACEND010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.242"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            256..1740
                     /gene="guaB"
                     /locus_tag="IW746_002657"
     CDS             256..1740
                     /gene="guaB"
                     /locus_tag="IW746_002657"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1079530.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          595 a          304 c          399 g          448 t
ORIGIN      
        1 tcacggtgct caaaagctta tatctgcggt aataagtcca accaaatcaa aaacatgaga
       61 agctgttttt gattggctgt tcttattact tgatgcagga cagctttttt acaatctctt
      121 ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct aacttgattc
      181 caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa ctaaattaga
      241 ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg atttactttt
      301 gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt ggatatgagc
      361 gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag tatggatact
      421 gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg tgtgatccat
      481 aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg ttctgaaagt
      541 ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga cgcagaagaa
      601 ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga aaacagaaaa
      661 ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca catgccgatt
      721 gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc tctaaaagat
      781 gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga taacgaaggt
      841 cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt tccaaatgca
      901 gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac aagcgataca
      961 tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat cgatacagct
     1021 catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac ttttgcagat
     1081 gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt atatgatgca
     1141 ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac acgtgtggta
     1201 gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt tgctcgtcaa
     1261 tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat cgtcaaagca
     1321 ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac agatgaatca
     1381 ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg tatgggctca
     1441 ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt caatgaagca
     1501 aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag tgttgcagat
     1561 attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg ggcagccaat
     1621 ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg attaaaagaa
     1681 tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt tgaatcttaa
     1741 aaacat
//
LOCUS       DACEND010000232         1738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEND010000232 DACEND010000000
VERSION     DACEND010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.243"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            164..1720
                     /gene="rny"
                     /locus_tag="IW746_002658"
     CDS             164..1720
                     /gene="rny"
                     /locus_tag="IW746_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1079531.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT          609 a          298 c          404 g          427 t
ORIGIN      
        1 tgtttattta aagttgacac tacggggtac aaacattaga ataaagttgt gcatgttttt
       61 tgtacacata tatgcgaaga tgcatattga cgagtttaga ataatttatc atagaagctg
      121 tatagatgat aaaaatgaaa aagatagtat ggaggtggat tttatggtat tcaacattct
      181 cctcgctatc atcggtttaa ttgtcggtct tggtttaggg tttatggtcg caaagtcacg
      241 tcacgagaaa gaaatagctg gtgcacaatc atcagctgct ggaatcatcg atagtgcaaa
      301 aaaagaagca gaaactctaa aaaaagaagc attgttagaa gctaaggaag aaaatcagaa
      361 ataccgttct gaagttgaaa gtgagctgag agaaagtaag ttagaattaa aatctcaaga
      421 gaatcgtttg attcaacgcg aacaaacgct gaatcgtaaa gatgactcgt tagaaaaacg
      481 cgagaactca cttgaaagta aagaagaagc acttagttca aaacagcaat taattgaaga
      541 gcgagaaaaa gacgttgaaa aaatgattga agatcaacaa aaagaactag aaaaaattgc
      601 tggtttatca aaagaggacg cacgagatgt catcatgaaa tcgactgaag aagagctgaa
      661 tcatgagtta actctaatgg taaaagaatc tgaacagcgt gccaaagaag aagcagatcg
      721 caaagcgaaa aacttattat cattagcgat tcaacgctgc gcagcagatc aagtttcaga
      781 aacgactgtt tcagtcgtta cccttccgaa tgatgaaatg aaaggtcgta tcattggacg
      841 agaaggtcgt aatattcgaa cgcttgagac gctgactggt atcgatttaa tcatcgatga
      901 tacaccggaa gcagttgttc tttccggatt cgatccaatc cgaagagaaa tcgcacggat
      961 gaccttggaa aaactgatac aagatggacg aatccatcca gcgcgtatcg aagaaatggt
     1021 cgagaaatct cgtaaagaaa tggatgaacg tattcgcgaa tacggggaag aggcagcgtt
     1081 tgaagtcggt gctcatacac ttcatccaga tctaatcaag atcatcggac gtctgcgatt
     1141 cagaactagt tatggacaaa acgtcttgaa gcattcggtc gaggttgcaa aattagctgg
     1201 tatcttagca gcagagcttg gcgaagatat ccaattagca aaacgtgccg ggttgctcca
     1261 tgatattggg aaagcacttg accatgagat tgaaggatct cacgttgaga tcggtgcgga
     1321 attagctgcc aagtacaaag aaaacccagt agtaatcaac gcgatcgctt ctcatcatgg
     1381 agatgtggaa gctacttctg ttatctctgt ccttgtagct gcagcagatg cgctctcagc
     1441 agcaagacct ggtgcgcgaa gtgaatcatt agaaaactac atccgtcgtt taagaagtct
     1501 ggaaaatatt tcgaacagct tcgccggtgt agaatcaagt tacgcagttc aagctggacg
     1561 cgaagttcgg gtaatggtca agccagaaga aatctcggat ttagacgctg ttcgtttagt
     1621 acgtgatatt cgtaaacgaa tcgaagaaga actggattat cctggacaca tcaaagtaat
     1681 cgttattcgg gaaactcgtg caactgatta tgctaaataa taaaaaatac aaaagaag
//
LOCUS       DACEND010000233         1711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEND010000233 DACEND010000000
VERSION     DACEND010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.245"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..243
                     /locus_tag="IW746_002659"
     CDS             <1..243
                     /locus_tag="IW746_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079532.1"
                     /translation="MEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEE
                     FALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            304..>1711
                     /locus_tag="IW746_002660"
     CDS             304..>1711
                     /locus_tag="IW746_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1079533.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILISYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQ"
BASE COUNT          649 a          260 c          363 g          439 t
ORIGIN      
        1 atggaagaag atacgggtcg aacgttacat ttgaaggatg cggcgtttat tgaagatcca
       61 cagcataatc aagtaatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta
      121 ccttcagaag aatttgcgct aggaaattca ttccgatttt cacttgagac agtgtcagaa
      181 gtggcaaaac gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgtgta
      241 taaaacaagt aaaaaagact atctgaaaaa cggatagtct tttttctttt gggaggtttt
      301 aaaatgaccg agaaaaaaat gagtcttatt gatcgatgta aacagatcga tatagtggat
      361 tttgctcgaa acaatggaat ggctgtagtc aataaaggac gagactatcg cttagaagac
      421 catgattcat ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt
      481 agtggcgaca ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct
      541 aagcaacaat ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga
      601 gtggaaaatc ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca
      661 ctcacagaaa aaggaaggaa ttatctgaag gaagaacgaa aactaccaga gtggctgatt
      721 gattatgcag aaaaagaagg gttgattgct gaactaaagc cgaaacatga acggcaaaat
      781 tttttagttg gagacgaccg tttagatcat gcggttgctt ttttatggaa agatccacag
      841 acaggagaga cagtaggggc aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt
      901 aaacggggaa cgtataaaca cattgacaag aatcctacgc caaatcacgg atttaattta
      961 aaaataggcg atccgaagca tttaaaattc tttgaaagta gtattgatct gttaagttac
     1021 gctgcattga atcgggagaa actacaagat gcctggttag tttcgatgga cgggttaaaa
     1081 catcatgtaa tcagtcatta cgttgaagag tcgatttctg aattaagacg aaaacaaaca
     1141 tttcctcaat cgattgaaat atgtgtggac aatgatcgtg caggacatat tttttatgaa
     1201 aaagaacaga tgaagggaat cgttgatcct tttaccaata agaaaatccg ttgtgagcga
     1261 ggaattccaa atgattggca agtacctaaa gaatacaaag ctacttatga agcagtcgca
     1321 aaagaaatga gtgttgaacc tgaagcaatt atggccatac acaaaactga aactaattta
     1381 cagctgacaa atcaattggt gtctgctcat gatgttcaat ctacgtttgg aaagatgttg
     1441 gcgaagggcg aaccagtaga aacaattgat ttgaaagaag catgtaccac ggtcgcgaag
     1501 gaactgaaag tatgtgaacg cgcagatgga acatacaact ttgatcgttt ttatagtaga
     1561 aaagccaaca taaaagacgt taatgcagga atccttattt cctataaagc agaacaatac
     1621 tacaaaggct ataaaaagca tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat
     1681 gatcaattga agcatgagat ccagcaacaa g
//
LOCUS       DACEND010000234         1704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEND010000234 DACEND010000000
VERSION     DACEND010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.246"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(205..>1704)
                     /locus_tag="IW746_002661"
     CDS             complement(205..>1704)
                     /locus_tag="IW746_002661"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1079534.1"
                     /translation="IDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKEHYGDSN
                     IGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLGDWGTQF
                     GKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRLEEGDKE
                     AIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHLLNEDKG
                     AEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNEQSYHFK
                     QLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQSAKEQIS
                     EKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYVQYTHAR
                     AVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIAKHAIKL
                     AQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT          456 a          366 c          296 g          586 t
ORIGIN      
        1 aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa acaaacagtg
       61 tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca
      121 aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa
      181 cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg
      241 aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg
      301 ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt
      361 gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc
      421 agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc
      481 tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt
      541 atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc
      601 aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg
      661 tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc
      721 agattggatt gcttcattta atacttcttc taataagacg atctttcctt tacgagttga
      781 taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt cgtcagacca
      841 gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt
      901 ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc
      961 taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag
     1021 gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc
     1081 ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga
     1141 atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg
     1201 gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt tgaaccaagc
     1261 acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacgtataa
     1321 acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta
     1381 agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg
     1441 ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa
     1501 atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc
     1561 ttgattcccg atgttgctat ccccataatg ttctttttct ttcactacgg tttgcaaaac
     1621 ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat
     1681 tttttcaaag ttggcagaat cgat
//
LOCUS       DACEND010000235         1702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEND010000235 DACEND010000000
VERSION     DACEND010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.247"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..243
                     /locus_tag="IW746_002662"
     CDS             <1..243
                     /locus_tag="IW746_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079535.1"
                     /translation="LEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEE
                     FALGNQFRFSLETVSEVAKRKNEIYYEQQMSQKERV"
     gene            304..>1702
                     /locus_tag="IW746_002663"
     CDS             304..>1702
                     /locus_tag="IW746_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1079536.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYIIKDENKVERVEN
                     LHFETQEYKHHPIDYRPLTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNY
                     IVQDDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTI
                     YEEVGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSE
                     VKKDWNDQLKHEIQQQ"
BASE COUNT          614 a          265 c          377 g          446 t
ORIGIN      
        1 ctggaagaag atacaggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg
       61 cagcataatc aagtgattct aaaagggaaa agttatggga aagagatcca aacggcttta
      121 ccttcagaag aatttgcgct aggaaatcaa ttccgatttt cactagaaac tgtatcagaa
      181 gtggccaaac gaaaaaatga aatctattat gaacaacaaa tgtcacaaaa agaacgagta
      241 taaaacaagc aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg
      301 aaaatgacag agaaaaaaat gagtcttatt gatcgatgca agcaaatgga tattgtggat
      361 tttgctcgaa acaatggttt agcggtagtc aataaaggac gagactatcg tttagaagac
      421 catgattctt ttgttttcga tcgaagaaaa cagcgatttt attggaacag tcaaggtatt
      481 ggtggggata ttattgaatt gactcaattg ttttttattg acgattctat tcaaaaccca
      541 aagcaacgtt ttaaagcagc gctagattat attataaagg acgaaaataa ggtggaacgg
      601 gtggaaaatc ttcactttga aacacaagaa tataagcacc acccaattga ttatcgacca
      661 ttgacagaaa aaggacgaag ttacttaaaa gaacaacgaa aattccctga atggttgatt
      721 gattacggag aacaacaagg gttgatcctg gaaatgaagc cacgcaatga acgaaaaaat
      781 tatattgtgc aagatgatcg cttagactat gcgatcgttt ttccctggaa agaccgtcat
      841 acagaccaaa tattaggggc aacttatcaa gggactatcg tagactatga acgttttgga
      901 aaacggggtg ttgacaaacg aatagatata aatcctacgc ctaatcacgg gtttaatgtt
      961 cttcttggat ctcctaagaa tctgaaattt tttgagagtc ccattgattt acttagttac
     1021 gctgctttaa atcgcgagac gatcaaagac acgtggcttg tttctatgga tggactgaag
     1081 gatgcagtag tggggcacta tgttcgagaa gcggtgacag aattggcgcc gaaagaagaa
     1141 tttccagaat cgattgagat ttgtgtggat aatgatcgag ctggacacgt attctgggat
     1201 aaggtacacc gacttggtgt gtcgaatcca tttacaggaa aagcggtgta ttttgaaccg
     1261 aatcttccca acgattggca agtaccgaga gaatatcaaa caatctatga agaagtaggg
     1321 aaagaaatga gagttgcgcc tgaagcaatc atggctattc ataaaatcga aaacaaccta
     1381 acggatacta accaaatcgt atcgttcggt gaaatccagg gagtatttgc caaaaaactt
     1441 gcgccaaaag agaaagccca atctattgtt gtaaaggaaa aatgtataga agcagctcag
     1501 cagctaaaat cgtgtgaaaa atctgatgga acatacgatt ttgatcgttt ttatcgtgga
     1561 aaagggaaaa ttaatgaaca aattcaattt tcattgaagg ccaaacacta ttttgaaggt
     1621 tataaaaatc atgaacacga atttgtttct gaagtgaaaa aagattggaa tgatcaattg
     1681 aagcatgaga tccagcaaca ag
//
LOCUS       DACEND010000236         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEND010000236 DACEND010000000
VERSION     DACEND010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.248"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..1484)
                     /locus_tag="IW746_002664"
     CDS             complement(18..1484)
                     /locus_tag="IW746_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1079537.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          529 a          328 c          277 g          530 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttctttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac aattggtgtg
      121 atgatattcg tgaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaagat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcgc tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgctcct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccgcc agcaatcagg attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgaccc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat ttaccttatt gaat
//
LOCUS       DACEND010000237         1660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEND010000237 DACEND010000000
VERSION     DACEND010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.249"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW746_002665"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW746_002665"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1079538.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..>1660)
                     /locus_tag="IW746_002666"
     CDS             complement(1172..>1660)
                     /locus_tag="IW746_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1079539.1"
                     /translation="FGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVPGSLEILKKVHP
                     HADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFFDHVFSHIPNFD
                     KKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDSLEELYSLLEIE
                     KD"
BASE COUNT          471 a          350 c          292 g          547 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa
//
LOCUS       DACEND010000238         1630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEND010000238 DACEND010000000
VERSION     DACEND010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.250"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..315)
                     /locus_tag="IW746_002667"
     CDS             complement(<1..315)
                     /locus_tag="IW746_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079540.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGR"
     gene            958..>1630
                     /locus_tag="IW746_002668"
     CDS             958..>1630
                     /locus_tag="IW746_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1079541.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFK
                     SITFDC"
BASE COUNT          567 a          277 c          284 g          502 t
ORIGIN      
        1 gcgtccgtga ggcgtgctgg tatcgaagtt ttctttcaag ctcaccagct taacatggtg
       61 ttcttcaaaa taggtaacaa ggtcaatcaa atcctttgta ctacgaccca atcgagaaaa
      121 actttctaca attaccgtat ctcccggacg aattttatct tttaaacgat ttagttgggg
      181 acgaaccgct ttggttcccg tcattttctc agttaggatt tcattacaat tttgttcatt
      241 gagtaagtct aattgacgat ttagttcttg tgaacgtgta ctcactcggg cataaccata
      301 aatgtatcca ctcatgcttt tcttcctttc gtactttaat tctggcttga ttgtatcata
      361 aaagggagtt atagatacat agttaatgca acaagttatg atacaaaata tgacctcttt
      421 tatttaataa aagaggtcat ttcattttgt ctcataatcg attgtttttg atacgagtgt
      481 gtatacgaaa taaacaaaaa aatcttaagt aaatttctag atacctaatt taatcctaac
      541 tagtgtatcc taaagttaat aacgagttta tcacaatgaa aattataaaa tgctcccata
      601 ttagatataa catgcaatat ttgaattaga actacaaaat ttattcgttt tataaagtaa
      661 ataatgttga gatactaagg aatagaatat tataaggaat tgagctgaat gtattggata
      721 aaaaaagaat aactttatta gagatggact ataaaaatgc tcgtaatttt cttctgaagt
      781 ctgaaagcta tttgaatgct cagctaccag aatatattag ttttgacgaa actttaaaat
      841 ctgctaaaaa agttttaact tcgccgtttg tataattaag cgagacaaat aaaaaagcca
      901 tttatgttac actcaaattg taatacccgc taaagcatac aaggagagtg aacataaatg
      961 acttataccc atcttacacc aaacgagctt gtaatgatag aagcatattt tcatcaagaa
     1021 actccggttg ctatcgttgc gaagcagctt aaacgtggac gccaaacaat ttacaatgtc
     1081 tataactttc tcaaatgtgg tggaacagca cttgaatact ttgaacaata caaagaaaat
     1141 aagcgacgtt gtgggagaac cgaaatcatt tttcctgctg aggaaaaaga atacattgaa
     1201 aaaagatcaa ctgaaggttg gaccccagac gtcattattg gccgtgcaga gcgaaccttc
     1261 tcttgttcag taagtaccct ctatcgccgg tttaagacgg gagaattcaa tgttttacat
     1321 ttaccgatgc aaggaaaacg aaaaccaaat ggttataagg aaaaacgtgg aaaacaggca
     1381 ttcaaaagaa atatttctga acgtaaaaaa gattatgtcg ttttcgaaga agaatttgga
     1441 catttagagg gtgatacgat tgtcggcatc caccataaaa gtgccgtcat cacactcgtt
     1501 gagcgacttt caaaagccat tatcgccttg aaaccagaag gccgtaaggc agttgatatt
     1561 gaaaattcga ttaatgaatg gcttcaatcc gtacccaaaa atcttttcaa atcaattacc
     1621 tttgattgtg
//
LOCUS       DACEND010000239         1581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEND010000239 DACEND010000000
VERSION     DACEND010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.251"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW746_002669"
     CDS             200..742
                     /locus_tag="IW746_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079542.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW746_002670"
     CDS             898..1347
                     /locus_tag="IW746_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1079543.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1581)
                     /locus_tag="IW746_002671"
     CDS             complement(1377..>1581)
                     /locus_tag="IW746_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1079544.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          600 a          217 c          308 g          456 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt g
//
LOCUS       DACEND010000240         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEND010000240 DACEND010000000
VERSION     DACEND010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.252"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..1433)
                     /locus_tag="IW746_002672"
     CDS             complement(141..1433)
                     /locus_tag="IW746_002672"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1079545.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          445 a          338 c          273 g          522 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaa
//
LOCUS       DACEND010000241         1566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEND010000241 DACEND010000000
VERSION     DACEND010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.253"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(342..415)
                     /locus_tag="IW746_002673"
     tRNA            complement(342..415)
                     /locus_tag="IW746_002673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Ile,seq:cat)
     gene            complement(604..1563)
                     /locus_tag="IW746_002674"
     CDS             complement(604..1563)
                     /locus_tag="IW746_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1079546.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          462 a          305 c          277 g          522 t
ORIGIN      
        1 cttttttctc actttttttc aaaaatatgg tatgcttttt aatggcttca aatataaaag
       61 agtttaaagc gtaacacact tatggggcag tggtttttgg ggaacgcttt aaactcttct
      121 ttattattat ctcacaatat aacccaaatg tctttctatt taaaaatcaa agtaaaactt
      181 ttcaaatata cagaaatata actatgtgaa acatcctttc attaatccat aaaaggatac
      241 ataaaaaagc cactcatttg agtggcaatg aagaaaagct ttagcttgta taatactctt
      301 caaaaaattc taacacagaa cgattcaaat ggctacgtta atgtaccctg taggactcga
      361 acctacgacc ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtactggtt
      421 gttgccacat aaagccataa acaatcaacc agtagaatgt gtggcaacaa acctgttatc
      481 gcatagcttg gagtgtgact attatgggtg atagtgaaga tatgcgataa catcactatt
      541 ttatcgaatg atttttatag ttgtcaatat agttatgtaa tgctcctcaa cgaggagcta
      601 tttttatcgt ttaggaatat ttaaatacca acgtttgtca tggaaatctt gcgcaccgcc
      661 tttagtgttt ccctctggat cattcgttgc ccgcatcatg acatagactt tcttattagg
      721 gaagttacgc atgttaaaag atacatgata gccaacattt ccagaagtat tataagcttg
      781 atttacatct gatctataaa ttccatcagc tcttactcga gctaattctt tcccagtatt
      841 gtaatccata atgaagatat actcgtattt atagttagca atgtgccatc cagctacatg
      901 caaatttgca ttttcgattt ctccaaactg atcaatgtgg gcgtaattcg ttccatctgt
      961 cagtgtagga ttagctgcac cagctcgtgt tggatcaatg acaggcttgt tttcagaagt
     1021 tgttggattt tcatcggtaa atccatgagc taaatcatat gctaattttt ctttacttac
     1081 gcccatttca gaaagataac cgtaaggatc tgtatgatcg ccccagatgt tttgtgttac
     1141 ccataaatgc gatttgattc ccggttggtt ataaggagtg tccaacgtta atggaatacc
     1201 atatttcatt gctgaatctc tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt
     1261 tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga ctgttggcat tagcatacga
     1321 accagcaccg tactgtacat aaccaggttg accgacttga taaacaattc cgccgtctcc
     1381 cacaatataa gcagtataag cgctagtcca tgaacgttgc atatactgcg cttcattgcg
     1441 tcctgttgct gtttcattag ccgtttcatg cagtaaaata tactgattat ttgctacttg
     1501 tgagctacct tcatttgcgc ccaaattaaa ttcattgttg atagtatagg caaatccgtt
     1561 aattgg
//
LOCUS       DACEND010000242         1533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEND010000242 DACEND010000000
VERSION     DACEND010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.254"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..231
                     /locus_tag="IW746_002675"
     CDS             <1..231
                     /locus_tag="IW746_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079547.1"
                     /translation="MELDSELLYQFYLAFRGQQISFPMKMYDRELVRKRIENMIEQEK
                     TVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            224..520
                     /locus_tag="IW746_002676"
     CDS             224..520
                     /locus_tag="IW746_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1079548.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            595..>1533
                     /locus_tag="IW746_002677"
     CDS             595..>1533
                     /locus_tag="IW746_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1079549.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVY"
BASE COUNT          589 a          218 c          304 g          422 t
ORIGIN      
        1 atggaactgg attcagaatt gctctatcaa ttttatcttg cttttcgagg gcaacagatt
       61 tcttttccaa tgaaaatgta cgatcgtgag ttagttagaa aaagaatcga aaacatgata
      121 gaacaggaaa aaactgtaga tatccgtcaa ttgacagaag tttatggttt ctctgcaaga
      181 tggattcaaa aagtaattaa acaaaaagaa aggagacgag tacatggcta agctgattgt
      241 attaggtggt ttaatttacg gtggatattg gttatataaa tatatgcttg cgcatccgaa
      301 tatagataaa ggaaaagtaa ttaagacagt tggggtaatc atagttggac tcatagctct
      361 aactaagaaa aataatacta atagcccaaa aataatgaga acttatcgct gtagaggatg
      421 tggaagggaa atttctacta gttttaatcc atctgagagt cctcaaggaa gagaccgctg
      481 ccatacgggc tatcgacatt attgggaaag aatcgattaa aagattacca tttagatcaa
      541 ttaattgtct gatagaataa gtacgtgagg gagaaattga aagggggaaa tataatgaaa
      601 aaatggctgt tattactaat aggaatggga attttattca ctggttgtac atctgagaat
      661 acgaggacta aagcgactat agaaactgat acaacaattg agactaaaaa aagtaaaacg
      721 caatcaactg aacaaactac aacagaaatg aagcaatctg tggaaacaag tgcaagttca
      781 atcactgaat cttcttctga agaggttaag aatactttgt gggatacgaa taaagcttcc
      841 aaattagaaa cttttgtcac acaatgggga aagacattag gtcaagaata taaaagctac
      901 aatcttcaga ataatgtaag tctgtatggc actcctttac cacaagccgt gattaatggc
      961 gattggaaaa tggcaatcaa tgaagcacca gttaccgtac aatggtcaga agatggaact
     1021 ggtcatgcag atttcaattt agtagcagtt tattctgatg tagaaactgg agaatattta
     1081 gggcagcatg tttacttttt cggttttcaa aatggacaac ctaaagttta tttaacgcaa
     1141 caaaatcaag gtaatgaaaa taattatctg tactttaacg aaactcaaaa tcaacagtta
     1201 aaacaaggtt tcagtgatat tgtgaatgga caaactcctc aaacaccagt agtagaagaa
     1261 tctactcaac aagcaacaac tacaacgaca caatctacaa gtagttcagg aatttcaagt
     1321 tgggaagctg ctaaagaatc tgtgctaaat aataaagctt tatggttgta tgattctcct
     1381 gataaaaatg tatcaaatac taatttaatt agcacaaatg aatatatgaa agatctaatg
     1441 caagatgaaa atggaagcta ctattcaatt aatattgtaa cggatgatga tggaaacacg
     1501 accatggctg gtggaaaaca atttcgtgtt tat
//
LOCUS       DACEND010000243         1531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEND010000243 DACEND010000000
VERSION     DACEND010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.255"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..1481
                     /locus_tag="IW746_002678"
     CDS             15..1481
                     /locus_tag="IW746_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1079550.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          500 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctgtttt t
//
LOCUS       DACEND010000244         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEND010000244 DACEND010000000
VERSION     DACEND010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.256"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(185..1168)
                     /locus_tag="IW746_002679"
     CDS             complement(185..1168)
                     /locus_tag="IW746_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1079551.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT          470 a          279 c          231 g          550 t
ORIGIN      
        1 gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa agaatgagac
       61 aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc aatagataca
      121 aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt tattttaata
      181 taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact ctatcaatta
      241 tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg tcaatataaa
      301 gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct aatacccacc
      361 ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta ggtttgaaca
      421 tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct ttatttaggc
      481 ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat cctgccgtcc
      541 cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc attgtaaact
      601 gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc cctataggat
      661 caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga tacaacaagc
      721 cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata ccttttcttc
      781 ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact tttgtccatg
      841 gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt
      901 aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat actttatcat
      961 cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat acaatacttt
     1021 tcgttgctga catgatatga caagtatcgt cttttttata accattccat tcatctgtat
     1081 aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt cgaaaaccaa
     1141 taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc tataagcata
     1201 atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg tattcttatg
     1261 gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc actttatgag
     1321 tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta gtgaaaatcg
     1381 attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta taaagctttt
     1441 tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt aaaggtgact
     1501 atggtaagat aatagagata ttattttgtg
//
LOCUS       DACEND010000245         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEND010000245 DACEND010000000
VERSION     DACEND010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.257"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..883)
                     /locus_tag="IW746_002680"
     CDS             complement(<1..883)
                     /locus_tag="IW746_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1079552.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDA"
     gene            complement(883..>1524)
                     /locus_tag="IW746_002681"
     CDS             complement(883..>1524)
                     /locus_tag="IW746_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079553.1"
                     /translation="EYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEK
                     QRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSP
                     QRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKEYE
                     QQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT          384 a          303 c          266 g          571 t
ORIGIN      
        1 tggcatcata ataaatcgta ttcgtgaaag tattggttaa ctgtgtcgct gtgtctatgt
       61 ctcctcgctt ccagtatttc acgatgagtg ccaatggatt atcactcatt gattgcagtg
      121 gatcggtgat attaaatgca tacacatcat accctctagc ttctagttca tctttactag
      181 caacatataa ttctgttttt ggatcattaa caaccattga actttgtctt tctgcacgag
      241 acaaattgtc aatggttgct aaaacttcta tctgtccttt ccctgaacgg gaagtaccaa
      301 caatacaagt atttactgta tccgtctgaa tataccagta ctttttataa tgagtaattg
      361 gtattccgcc ataaccagga aaacctccct ttgattctgg tatttgcgga taaatttgtt
      421 ggatttcctt taacgttttt aatcggttat ctcctttttg cccatctgct agaggacgaa
      481 accacatgtc cattttcaat cccataagta cagctggaac aacacaaatc gtagaaacaa
      541 ctaaacgtgt gcctaaactt gttgttgggg tcaaaggatt ccaaaaacca gttgaatcaa
      601 aatggaataa ctgcggcatt tcttgtaact gaaaaatatt cactccttgt aaacttcctg
      661 gaataatctc aaatcgatga aatgcttctg gtattcttgt aacaacatag tttaaaggaa
      721 gataagctaa gccactataa aaaatctttt gagaaatacg tttgtcatgt ttttctggta
      781 gctcttctcc tgaaggagta tagcgataga aatgagaaag atacctattc ttttctaaat
      841 tgaatcgttt gtcttttcgt ctcgttgttt ctctaacacg cattatttca cctcatttga
      901 ttctgaactt tcggaagtgt ttgattgtac agttgaatcg cttgatcctt ctttagcctt
      961 actaggatca ctctctttgc tattttccat acttgattct gttgttgact gattggtacg
     1021 attcaataat tcttctaatt gttgttcata ctcttttctt gcttgttctt gggcttcttc
     1081 cttgattgac gtcgttgtgg ccacagaatc agcctgtgaa gattgattga tatgattaaa
     1141 agcaaatact gttcctccac ctagaagcaa gcttaaactt gcaatacatc ccaccatcca
     1201 tccgttaaag gaacgctgtg gtgaaggagt tagatttgtt tggttgttca ttggggtttg
     1261 aacagcagct actgaaggcg ttggttgagg ttgtgctaga taatttaact gagcttgtcg
     1321 caatgtatcc atttgttctt gacgttcctt acgagcagct gcttcttttt ccttttcttg
     1381 ctctaaccgt tgtttttcta tcgccatttt ttgttccgca agatcctgtt cccgttttct
     1441 tatttctaat tgttcacgct ctttttcttt ttgccgttgt ttttcttctt gttctttcaa
     1501 ttcttgttgt tgaatttcat actc
//
LOCUS       DACEND010000246         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEND010000246 DACEND010000000
VERSION     DACEND010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.258"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..391)
                     /locus_tag="IW746_002682"
     CDS             complement(<1..391)
                     /locus_tag="IW746_002682"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1079554.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIA"
     gene            complement(403..1392)
                     /gene="galE"
                     /locus_tag="IW746_002683"
     CDS             complement(403..1392)
                     /gene="galE"
                     /locus_tag="IW746_002683"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1079555.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT          383 a          325 c          260 g          507 t
ORIGIN      
        1 ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag aaatactcag
       61 ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg
      121 gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct ggcaattctg
      181 aaagaacacc attttctctt gccttagcga caagctgatc caataagtct acagattctt
      241 ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc cctaaaagac
      301 gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc gcaagtgtgg
      361 caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt cataaccatt
      421 tggatggctg acatgccaat cccatgctgt tttgataatc gctttgatat ctgtgtattc
      481 aggtttccag cccagaatct ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc
      541 tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt ctctagctgc
      601 ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga agttgctttc
      661 atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat cttctacttg
      721 aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat aaacagccaa
      781 ggcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg tttctggtgt
      841 atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt agcgcaaagc
      901 gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca tcatcaattt
      961 actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg gagactcttt
     1021 cggctctcca taagttgctg cagtagaaga gaatacgatt ttattcacgt tgaactcgtg
     1081 catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca tcaatggttt
     1141 ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt caatcggttc
     1201 cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat aaaaatgagc
     1261 atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag ctacttgata
     1321 ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc cgcctagtac
     1381 taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt gtgtttgttg attctatata
     1441 atttaaaaaa cgaagtttac ttttatggtg aaaac
//
LOCUS       DACEND010000247         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEND010000247 DACEND010000000
VERSION     DACEND010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.260"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..1392
                     /locus_tag="IW746_002684"
     CDS             100..1392
                     /locus_tag="IW746_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1079556.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACEND010000248         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEND010000248 DACEND010000000
VERSION     DACEND010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.261"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(10..417)
                     /locus_tag="IW746_002685"
     CDS             complement(10..417)
                     /locus_tag="IW746_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079557.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(436..1254)
                     /locus_tag="IW746_002686"
     CDS             complement(436..1254)
                     /locus_tag="IW746_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1079558.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          423 a          230 c          201 g          564 t
ORIGIN      
        1 gaaaaaaaat tattttggta atttttcaaa ctcttcttgt atttcttctc ttatgtgttc
       61 ttcttttgct actcctagtc tatgctggat atatgagtct gctaatgtat taacaatttt
      121 ttcgaaagaa attctccctt tttcactttg taactccgtc atatagtctt gtaaaattga
      181 gagtttgcta tttaatgccg gagagatctt tttaggaact gataccttgt ttcttttatc
      241 aggaattgaa actggtcggc ctcttttttt caatgtaata ttttttgtgt tactcaatgc
      301 actattgcta aagttatatt caccgcttat tttcacatct tggcgtttgt tattgggtaa
      361 taatggctca aattcgccat tctccatcat tttaggaata tcaaaatctt taggcattac
      421 ttatgcaact ccttattatt tatttaaaaa ctcttgtcta gcatctagtt ctgccaaaat
      481 atttataaat acttggtgtg ctttataatc ccattgatca aaatttccat ttttgtattt
      541 tcttaaatgt ataccaattt tagaatacct tctcaatctt ttttgataat gaatgagatt
      601 tttggatacc acggttccgt atttttcaat tgcttcttct aggatttctt catcaaaatc
      661 atcatttggt tctaccatac aaggtacaat atctataatt tgcaaatcaa gatcgtatct
      721 atcgaccatg aatttttgat aatttacata tttgttgact ccctctaagg attcttcagc
      781 tgtttgaaat gaaattatcg aatagtctga agctgccata gcattatcgg aaaattcgct
      841 aatggttggt ggtacatcaa taaatatttc ttgataattg ttctttattg gaagaagtaa
      901 ttttttcaag acagtaactt ggtctaattc attttcatgt ttattagcaa aagcggagaa
      961 tgatcgaaaa ctagtgtcac atgccagaag gtcaagattt tcatttacct taataatact
     1021 acttgctaat tctccatttg cgacaccatt tgttatagtg gttttgatat cggttatacc
     1081 gaaagttctt gctatgactt gtgttaagtt cgcttgaggg tctaaatcaa taataagcgt
     1141 ttttctattt cttatcattg cattgtcata agcaagcatg ctgactatct tagttttacc
     1201 tactcctcct ttaaaattgc caactgtaaa tgtagtccct tgaaaagtat ccatgtaaat
     1261 cctcccaatc tttaattgat aattaaagta taacttatat atcggaaatg gtcaacatta
     1321 aaagtataac ttaaataata atatctaaaa acgtttggat atagcggttt ttaagaataa
     1381 aagtataagt tataccctaa atatatatgg tataactt
//
LOCUS       DACEND010000249         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEND010000249 DACEND010000000
VERSION     DACEND010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.262"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..758
                     /gene="dnaI"
                     /locus_tag="IW746_002687"
     CDS             <1..758
                     /gene="dnaI"
                     /locus_tag="IW746_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1079559.1"
                     /translation="YEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALL
                     ARQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHK
                     GLYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAV
                     KRSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQ
                     RGDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            887..1327
                     /locus_tag="IW746_002688"
     CDS             887..1327
                     /locus_tag="IW746_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1079560.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT          486 a          233 c          323 g          365 t
ORIGIN      
        1 tgtatgaatt cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc
       61 ccggctatga gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa
      121 cggaagcatt gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg
      181 atatgccaaa agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag
      241 gatatgcgag cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt
      301 atcataaagg actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag
      361 caacagcgag agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat
      421 ttgccgttga gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag
      481 tgaaacgttc acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga
      541 tacgagacga agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct
      601 tcttctcatc gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg
      661 gagatgaaga gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag
      721 aaatcgaaat gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac
      781 atttgaccta gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga
      841 caatcaccgc aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt
      901 ttctagtata tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag
      961 caacacaggt tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa
     1021 aacggagaga caaagcatat ttgtcggaac gattgaaagt attactcaag tagaagcaga
     1081 agaagaaacg cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat
     1141 gggaacgtca ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt
     1201 ggacataaat gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat
     1261 catgacgatg tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa
     1321 caaataacca ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt
     1381 atatttttta aggagatatg ggaattt
//
LOCUS       DACEND010000250         1381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEND010000250 DACEND010000000
VERSION     DACEND010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.263"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..1268
                     /locus_tag="IW746_002689"
     CDS             102..1268
                     /locus_tag="IW746_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1079561.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT          491 a          227 c          258 g          405 t
ORIGIN      
        1 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
       61 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
      121 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
      181 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
      241 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
      301 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
      361 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
      421 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
      481 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
      541 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
      601 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
      661 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
      721 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
      781 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
      841 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
      901 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
      961 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
     1021 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
     1081 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
     1141 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
     1201 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
     1261 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
     1321 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct
     1381 t
//
LOCUS       DACEND010000251         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEND010000251 DACEND010000000
VERSION     DACEND010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.264"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..>1373
                     /locus_tag="IW746_002690"
     CDS             50..>1373
                     /locus_tag="IW746_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1079562.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEK"
BASE COUNT          539 a          175 c          236 g          423 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aat
//
LOCUS       DACEND010000252         1361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEND010000252 DACEND010000000
VERSION     DACEND010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.266"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1046
                     /locus_tag="IW746_002691"
     CDS             <1..1046
                     /locus_tag="IW746_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1079563.1"
                     /translation="INLSKPEKDPKQIAAEREAVKVDYPTCMLCMENEGYRGRLNYPA
                     RTNHRIIRMNLDGESWGFQYSPYAYYNEHSIILSEQHRPMKITKETFARLLRITEVLP
                     HYFVGSNADLPIVGGSILSHDHYQAGRHEFPMAKAPIEKLIKLEDYPQVIAGVVKWPM
                     SVIRLQSTEKNLLIEAANSVLEKWKAYSDETVSIRAFSADGTPHHTITPIARRRDNYF
                     ELDLVLRDNNVSEEHPDGIFHPHKDVQHIKKENIGLIEVMGLAVLPPRLKPELVEVEH
                     FLLGEENQMAEYHRPWAEQLKKEHPDLTKDTVTDVVQKSVGQVFARVLEDAGVFKRDE
                     KGQAAFERFTAIL"
     gene            1070..>1361
                     /locus_tag="IW746_002692"
     CDS             1070..>1361
                     /locus_tag="IW746_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1079564.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLL"
BASE COUNT          461 a          265 c          287 g          348 t
ORIGIN      
        1 caatcaattt atctaagcca gaaaaagatc caaaacaaat cgcagcagaa cgcgaggcgg
       61 taaaagtaga ctatcctacc tgcatgcttt gcatggaaaa cgaaggatat agaggtcgct
      121 tgaactatcc agcccgcacc aatcaccgga ttattcgaat gaatctagac ggtgaaagct
      181 ggggattcca atattcccct tatgcttatt acaatgaaca ttcgatcatt ttatcagaac
      241 aacaccgccc gatgaaaatc acaaaggaaa catttgctag actgcttcgg attactgaag
      301 tcttgcctca ttactttgtt ggttcgaatg ctgacttacc aattgttgga ggatcgattc
      361 tttctcatga tcattatcag gcaggacgtc atgaatttcc tatggcaaaa gcaccgatag
      421 aaaaacttat caaactagaa gattatccgc aagtcatcgc aggagtcgtg aaatggccga
      481 tgtctgtgat ccgtttgcaa tcaacagaga aaaacttgct tattgaagca gctaactcag
      541 tattggaaaa atggaaagca tattcggatg aaacagtatc tatcagagct ttttcagcag
      601 atggtacacc acaccatacg attacgccaa ttgcacgaag aagggacaat tactttgagt
      661 tggaccttgt ccttcgagac aacaatgttt cagaagaaca tcctgatggg atttttcatc
      721 cacataaaga tgtccaacat atcaaaaagg agaacattgg gttgatcgaa gtaatggggc
      781 ttgctgtttt accacctcgt ttgaaaccag aactagtaga agtagagcat ttcttattag
      841 gagaagaaaa ccagatggct gaatatcatc gtccgtgggc agaacaatta aaaaaagaac
      901 atcctgattt gacgaaagat acggtcaccg atgtcgtaca aaaatctgta ggtcaggttt
      961 ttgcacgtgt actggaagac gctggagtat tcaagcgaga tgaaaaagga caggcagcgt
     1021 ttgaacgatt tactgcgatt ttataaacgt aaaaaaggag gagagacaaa tggcaacaat
     1081 taaagacatt gcgcagttgg caggagtttc tcctgctact gtttcaagag ttctcaacta
     1141 tgatcctgat ctttccgttg gcttagccac aaagcaaaaa atttttgaag cagcagaacg
     1201 cttaaattat acgaaacatc gtaaaaatgc agcaaatact aagcaaacgc ttcgcctgat
     1261 ccagtggtac gacgatacgg aagagcttga agatctttat tatttatcta ttcgcctagg
     1321 aatcgaaaaa aaggcagaag aagcaaacgt tactttattg a
//
LOCUS       DACEND010000253         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEND010000253 DACEND010000000
VERSION     DACEND010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.267"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..1350
                     /locus_tag="IW746_002693"
     CDS             join(11..452,452..1350)
                     /locus_tag="IW746_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1079565.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACEND010000254         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEND010000254 DACEND010000000
VERSION     DACEND010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.268"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1337
                     /locus_tag="IW746_002694"
     rRNA            <1..>1337
                     /locus_tag="IW746_002694"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACEND010000255         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEND010000255 DACEND010000000
VERSION     DACEND010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.269"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..787)
                     /locus_tag="IW746_002695"
     CDS             complement(53..787)
                     /locus_tag="IW746_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1079566.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..>1320
                     /locus_tag="IW746_002696"
     CDS             1114..>1320
                     /locus_tag="IW746_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1079567.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVP"
BASE COUNT          444 a          245 c          234 g          397 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
//
LOCUS       DACEND010000256         1317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEND010000256 DACEND010000000
VERSION     DACEND010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.270"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW746_002697"
     CDS             <1..111
                     /locus_tag="IW746_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1079568.1"
                     /translation="RKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            138..437
                     /locus_tag="IW746_002698"
     CDS             138..437
                     /locus_tag="IW746_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1079569.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            611..1162
                     /locus_tag="IW746_002699"
     CDS             611..1162
                     /locus_tag="IW746_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1079570.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
BASE COUNT          475 a          185 c          272 g          385 t
ORIGIN      
        1 agaaaagagt atctgatttc acctaagctt cgtttgaaat cagtgtctca aaaaatatgg
       61 gccattgatt cgggtgagta tcaaacgatg ttattaccag aagaatacta gtatctaaaa
      121 gaaaggaacg aaaggacttg ttagaaacgg ctatcgaagt actggaaaag tgtgcacagc
      181 tggtcactgc ttctgaagag tggggctatg aatccgttac gatggaaaaa gaggaaattg
      241 aaataggtac acttcctaaa gatgttcatc taccacgttt agtgatgaca catctttata
      301 tttattgcgc accagaagat ggaaaagact atgtagttta tttcattaca gatattacca
      361 gccagcgaga atttgttcgt gggttactgg tagaaggacg attggtttgg agtcagattg
      421 gaggaacgga tgaataagat cgattatttg agaatgattg cgaaatgttt agaagatatc
      481 cttaccgcga ttcatcaaat tctgaagaaa aaaggagaaa gaatatgaaa tattttcaac
      541 tagtattgac acttataacg gcaatggtaa ttgaagtgct tgaacaaaaa atagcatagg
      601 agaaaaagag atgattgaaa aagaattagt gaccacacaa acagaagttt ggcgagatcc
      661 tcatcatacc catcgctatg ttttcaaacg acaatgggcg atcaaaggaa aagaagcgga
      721 agataaatta gcagtagtca ttactattcg tccaacagat acagaaccat ttacgaatga
      781 tttgagtatg ttattaatcg aaaagaatat ccgacaatta ggatttactg gttttattgc
      841 tgtgaatctt ttttcgtaca caaaagcgaa aagtcctcgg gtatttcttc gaggaaatga
      901 tgaacaatct ttggaaatat taacgactgt ttttacagaa aagaaaatca ataccattat
      961 ttttgcttgt ggttcgatta cagcgacgag tcaagtggct tatgaacaag caaagactat
     1021 ttacgatcaa ttgagtgcga aacaaaagaa gatgaccaaa gtattgataa atgcagaagg
     1081 taagttatcc cacccactaa gtattcatgt tcgtaatgaa tggcaattgg cagatagtca
     1141 attattattt caagaaaaat aacattttat tttctcatgt tacttataag tagttttaca
     1201 tgctttttga ctttaaaaat acatgtgatg atacatgtta tatgaattat caatgtaccc
     1261 agtaatatgc tattttcatt atgtaaaaaa taacaggggg tgtgtagatg gagattg
//
LOCUS       DACEND010000257         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEND010000257 DACEND010000000
VERSION     DACEND010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.271"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..426)
                     /locus_tag="IW746_002700"
     CDS             complement(196..426)
                     /locus_tag="IW746_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079571.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(520..858)
                     /locus_tag="IW746_002701"
     CDS             complement(520..858)
                     /locus_tag="IW746_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079572.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          439 a          244 c          145 g          483 t
ORIGIN      
        1 aaacaaactt tagaaactct agaagaactt ctgaatttat atagaagtgt gtagctactt
       61 actaaatata caaaagttaa aataaaatac tctggatact ctttatactg cattaaagtt
      121 agtaatatta acagcaaaga tatcgaaaaa acaataacat aatatgtgtc aaaatttttc
      181 ataataagaa ctcccttact gttgcatact aacataatcc aaaaactctt ctacttcagc
      241 taaagacatt ccactttctt tagtccaaaa aacagttttt tctatcgatc cttctctgta
      301 aactgtaaat gtcggagtcg agttaattga gtagcgactc ttgaattctt tccactcatt
      361 ttcatcttct ctaacttttg ctacattcac gtaataaata ttttcttttc ccttttctac
      421 cagcatatcg aatagcttcc tgcttttgat gatcgatttc ttgtgctaca tctagtaaat
      481 tttcatcaat ttctatatca taaagtattt cactagtgtc tacatccaca atatatactt
      541 tttatcagta gcaatttttg agtaatccgt taaaatgatc tcagtctcgt cactttcatc
      601 agtataaata tacgttaaca atgaactcac cttttcaatc ggtgttagct ctaagcctac
      661 tgtatttttt actattgagt cattatttac caccataact tcgtactgta tataccatcc
      721 tatttggttt ccatcaatat cagtaattgg ctcactagct aatggagtct ccggaacaat
      781 attttcctga tatttcttaa actcttctgt agcacgaaaa aggttatcat gttcctcact
      841 tgaaactggt tgagtcatag aaaatgttac ttggctaaac agagggaaaa taaacactac
      901 aatcaacaaa gtataaaaaa aagcccttct ttttcatttt ttatcttcca atcacaaatt
      961 gaaaaactta ttcctgcaac tttgagtcta gtatcacaat cgactgacta caactattca
     1021 tttacattct actttaagtt cctccttaac cattaatttt attaaagata cttgtaatag
     1081 tgattctcac aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca
     1141 acttatcgac tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata
     1201 gtaactgctt ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt
     1261 tcgacaaata gaaagagata cgaatcactg atttttttga agaaaagaaa t
//
LOCUS       DACEND010000258         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEND010000258 DACEND010000000
VERSION     DACEND010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.272"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..>1310
                     /locus_tag="IW746_002702"
     CDS             129..>1310
                     /locus_tag="IW746_002702"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1079573.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          410 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat
//
LOCUS       DACEND010000259         1298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEND010000259 DACEND010000000
VERSION     DACEND010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.273"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW746_002703"
     CDS             complement(146..1129)
                     /locus_tag="IW746_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1079574.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          364 a          281 c          245 g          408 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccgga
//
LOCUS       DACEND010000260         1295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEND010000260 DACEND010000000
VERSION     DACEND010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.274"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..282
                     /locus_tag="IW746_002704"
     CDS             <1..282
                     /locus_tag="IW746_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1079575.1"
                     /translation="DLRGIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIA
                     LASKACGADIQHIGDSLNGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            301..>1295
                     /locus_tag="IW746_002705"
     CDS             301..>1295
                     /locus_tag="IW746_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1079576.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKM"
BASE COUNT          410 a          194 c          230 g          461 t
ORIGIN      
        1 gatttgcgag gaatcgttga ttatataaaa gattttaata gaaataaatc tattgaggaa
       61 aaagttctag tactaacggc taatggaaca agccaagact cttctgacca agaaactatt
      121 aaaaaaattg cattagcatc aaaagcatgt ggagctgata ttcaacacat aggggattct
      181 ttaaatggta tttgtcttcc agaaaatatt tttgctttgg gtcaagcaat tcggggatat
      241 cgtcatcaaa ttattatgtt aggaaaatct aattatcgtt aattaaggag gaaatgaaag
      301 atgatcgaca aagttcaaaa aatagctgca aaaattagta atcaaagaca tctactagct
      361 ttaagagaca gcatggctgc agttatgcca ttaatcatta ttggttcctt ttttatgtta
      421 ttagcaaatt tcccgataga aggatttaca aaatggttat ctgaaattgg agtacaatcc
      481 tttttgaaca aagcatcaga ttcaacattt ggtattattg gattagctgt tacctactct
      541 ttgggacaca atctagcaaa gacttatggt gtagactcat tatcagcagg attattatct
      601 ttgagtggat atgttttact tacaccttta ttggatgcag atggttctat aggatttcca
      661 acaaaatact ttggtacctc gggtcttttt gttggtatta ttgtagcact tgtatctaca
      721 gaaattttcc ggtggtttgt acttaaaaat attacaatta aaatgccggc aagcgttcca
      781 ccaaatgtaa gccgagcttt cgttgctatt attcctggct tttttgtcgt tctactatgg
      841 tttattgttg ttgtaatttg ttataaactt ggaattgaaa atgttcatgc tcttattgca
      901 gatacactcg caaaaccact atctttactg acgaaaactt taccaggaat tatattggtt
      961 atacttattc aatgtttctt ttggatgttt ggtattcatg gtgcacaggt aacggggcca
     1021 ataattgaac cgttattatt gcaaaactct gatgtaaata gaattgctta tcaagctgga
     1081 aaagaacttc ctaacattat aacttatgaa ttcttatata actttgtttt ttctggtggt
     1141 gcaggttgtt tatttgcact tgccatttta acatttttct tttcaaagag tcaggaaaat
     1201 aaaactttag gtaagttatc aatcgctcca gtaagttttc aagtagcaga gccccttcta
     1261 tttgggtttc caactatatt aaatataaaa atgtt
//
LOCUS       DACEND010000261         1285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEND010000261 DACEND010000000
VERSION     DACEND010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.275"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1073)
                     /locus_tag="IW746_002706"
     CDS             complement(<1..1073)
                     /locus_tag="IW746_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1079577.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSV"
BASE COUNT          360 a          266 c          238 g          421 t
ORIGIN      
        1 ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat
       61 aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg
      121 acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct
      181 ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt catacccgag
      241 atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga
      301 ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga
      361 gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct
      421 gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc ttctgctttt
      481 tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga
      541 gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg
      601 aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt
      661 gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg ataagacgct
      721 actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac gctgatcctg
      781 cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca
      841 atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga
      901 acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt
      961 gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt
     1021 tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa
     1081 attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa
     1141 acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt tcatgatata
     1201 tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg catatcaaaa
     1261 aaaacatgtt acaatgaaat taatc
//
LOCUS       DACEND010000262         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEND010000262 DACEND010000000
VERSION     DACEND010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.276"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW746_002707"
     CDS             complement(98..1228)
                     /locus_tag="IW746_002707"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1079578.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          358 a          250 c          208 g          463 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagca
//
LOCUS       DACEND010000263         1251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEND010000263 DACEND010000000
VERSION     DACEND010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.278"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..797
                     /locus_tag="IW746_002708"
                     /pseudo
     CDS             1..797
                     /locus_tag="IW746_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            complement(914..1057)
                     /locus_tag="IW746_002709"
                     /pseudo
     CDS             complement(914..1057)
                     /locus_tag="IW746_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            1139..>1251
                     /locus_tag="IW746_002710"
     CDS             1139..>1251
                     /locus_tag="IW746_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1079579.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEE"
BASE COUNT          446 a          211 c          210 g          384 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccca ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta
      901 attaatattg aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag
      961 gctcatcccc ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt
     1021 gttttctttc aaaagcgggc attggttagt caaagactag ttcatttata tttatatctt
     1081 aatcttttat tttaataaat tgtgatataa tgaagtataa ctttttaggg agtgtactat
     1141 gaattttcaa gataatagac cattatatct acaagtagaa gcttctatac gtaacgatat
     1201 tcgtgaaaaa aaatatcttc caggagagca attacctaca gaagaagaat t
//
LOCUS       DACEND010000264         1232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEND010000264 DACEND010000000
VERSION     DACEND010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.281"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..426
                     /locus_tag="IW746_002711"
     CDS             <1..426
                     /locus_tag="IW746_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079580.1"
                     /translation="AGMIEEMIYFYQLEAENLSNYGKLFEQAFYLTENGHLLSASMKK
                     FLGMMFHFVWNLQAYPFDFQLKNFFVWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSK
                     KILVLEKIGIIDGYQHPNEKELLRTMTVFQQIVSEIRGN"
     gene            428..>1232
                     /locus_tag="IW746_002712"
     CDS             428..>1232
                     /locus_tag="IW746_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1079581.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKE"
BASE COUNT          448 a          194 c          257 g          333 t
ORIGIN      
        1 gccgggatga tcgaagaaat gatctatttt tatcaattag aggcagaaaa cctgtcaaat
       61 tatggtaaac tgtttgaaca agctttttat ctgaccgaaa atggccatct actttctgct
      121 agtatgaaaa aatttttagg aatgatgttt catttcgtgt ggaatctcca agcatatcct
      181 tttgatttcc aactaaaaaa cttttttgtg tggctggact tgcttggcta tccaatccac
      241 tggctggaac agatttcaaa gatggagtat ttggaaaaag ttcttttttt ggcagaaaaa
      301 agcaaaaaaa tacttgtttt agaaaaaata ggtataatag acggatacca acatccaaac
      361 gaaaaagaat tattgagaac aatgacggta tttcaacaaa tcgtcagtga gataagaggg
      421 aattgaaatg aatacaaata gcgaaaaaat gttgcaagct ttatcagatg aagacttgtc
      481 acaagcacaa atctttttag aaaaagcatt aaaagaagac ccagcagata tattagctga
      541 actaggcgaa gaattgctgg ctattggctt tttgatcgaa gcaaaacaga tttttgaaca
      601 actgatcaac caatacccag aaaatgacgg actgaacatc ccgttagcag aaattgcaat
      661 cgaagataat gagatcgact tagcttttga atacctggaa aaagtggata agcaaagtga
      721 ttactatccg caaagcttat tggcgatagc tgatttgtac caagttatcg gaatccctga
      781 agtgagtgaa gcaaaattga aagaagcagc tgggttgctt cctgatgaac cattgatcca
      841 gtttgcgcta gctgaattgt atttttcagt tgatcgtttc aaagaggcag cagtggttta
      901 tgaaacattg ttggcaagcg ggataaatga aatttcagga atctcgatgc aggaacgttt
      961 aggacaatcc ttaagtatgc aaggagaatt cgaagcagct atcgtacctt tagaagaagc
     1021 actgaaagaa gaaaggacag atgatcgact gttccagcta gcttttacga atcttcaatt
     1081 gaaagaaaat cagcaggcaa tcaactatct ggaagaactt cgagaagtta atccgcatta
     1141 tcaatcgtta tatctatatc taggacaagc tcttcaagaa gaagaaatga ttgaagaagc
     1201 acaaacagtg ttagaagaag gaatcaaaga aa
//
LOCUS       DACEND010000265         1223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEND010000265 DACEND010000000
VERSION     DACEND010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.282"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..855
                     /locus_tag="IW746_002713"
     CDS             148..855
                     /locus_tag="IW746_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079582.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
BASE COUNT          443 a          185 c          250 g          345 t
ORIGIN      
        1 cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca
       61 agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat ggcgcctatt
      121 acaccatgaa aaaggaggac aacgaaaatg aataatgtag tttatcaact aacaatggaa
      181 gacatttctc gttttttaac aaacaaagaa catttgaatg aagtaatcag acggattcaa
      241 aaagacttta gttgtatgga agaaatgaag tactttgttc aaaacgttga agaacagtta
      301 tatttggaaa gcgaaacgag tcaaacaaag gtcacagtca gcaataatcg tttagatgtg
      361 tataaacgtc gtcctgaatc attaaaaaaa ggagcggagc ttctacgact agaaacagaa
      421 aaatacgtca tgaatgttta cctagaaaaa agtgagttgg tcgaaaattt tattagctac
      481 acgatttgca ggaaacacta ttactcagaa tcagaatatt attcaatttt tgagagcatg
      541 gaattaatgg aagaagaaat ggaagagtta aacagtaaag atgcgacggt agacgattat
      601 taccttgtat ttactagatt tttaacgtgt ggaacggaat ctatcgtgct aatgggaata
      661 gattacgaac tcgctgtacc cgaagcgttt ttgagggaat atgtagaaga agtagataac
      721 ccaggggatg attggtacac gactttaact gattttttag acacctacac ttacgacgat
      781 acagaagaaa tccaaaatga gcttgaccgt aaacaaatgc catatcaacg atttgaaagg
      841 aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaataa gaacaaaaaa
      901 ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct
      961 taggtggtgc gaaataagcg agcaaagccc gctaaccaaa gagaaagaag gagatttata
     1021 tgaaaaagat tattccctta ttattagtac tatttgttgg cgaaatgctt atgcgtcatg
     1081 ctgcaactgt gcatttaaac ttcgtagtat ttttggtaat actggtgttt tgtttgacct
     1141 tttggcggtt tgtattagcc ctcgtgagtt tactattcaa gatgattgca gccatatttt
     1201 tcataggatt atttcgatga gag
//
LOCUS       DACEND010000266         1197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEND010000266 DACEND010000000
VERSION     DACEND010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.283"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            211..1167
                     /locus_tag="IW746_002714"
     CDS             211..1167
                     /locus_tag="IW746_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1079583.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
BASE COUNT          428 a          219 c          176 g          374 t
ORIGIN      
        1 aaatatacca aaacaagata agtggtgtga atgtgcccat gaacgtaaac tcctttttta
       61 tcgttgcttc aagtatactg tatatacttt acacaactgt ttcattttat ttaaatttat
      121 gcagttatat tctatatctt acaaacacat tccatgtatt ccaattccct atccgttata
      181 ataaaagaaa caggcaacag gaggaaaatc atgatcaaat attttacatt agataatgag
      241 aaactgcgta ataccggaaa ggaaactgac caaacgatct ggtattgtgt agaacgtcct
      301 aatcaagaag agattcatca gctgacacat caatttgaaa ttccgtcaga ttacatcact
      361 tccatattgg acgatgcaga aaattcccgt acagaagaat tcaatcaatc aaagttgaca
      421 aagccagcgt tattattgtt acaatatcct tttgctaaaa caagtcctag cggttatttt
      481 caagtggata catacccgct ctctattatt ttgacgccca aaaagaagct tatcacaatt
      541 acgaatcatg aaccactgtt tttaaagaaa gtctttagcc agacctttcc aaaaagtgat
      601 atttcgccta atctcaatat cttctttcaa ttcatttggc aggtcacact ctcttacaac
      661 agttatttag ctacgcttca atctcaatgc gatactttgg aagccaaact tcaagtatcc
      721 actgaaaaca aacaacttta tcaaatcatg gatatccaaa aaagcctcgt ttatttcaga
      781 gaagcgactt ctgctaattt agatgcattg aaagaactag cgcaaaataa acttttccaa
      841 gaaaatcatg ctttgaaaaa tcatttacgt gatgtattgg tggaaacaga gcaggcaatg
      901 actacttctc gcattcaatt aaaactagta gaacaaatga atcagacttt ttcagcaatc
      961 gtatcgaata atctgaataa tattatgaaa attttaactt ctttaacgat tattttaact
     1021 ataccgacaa tcgtcggcag tatctatgga atgaacatta agttacctat tgctgaaagg
     1081 gatgatgcct ttatatggct agttctatta acattattta tttgtatcgt aactacctat
     1141 tatttaaaaa aagggaagtt tttataaaaa agccctcttg tggaaatgga aaaaaga
//
LOCUS       DACEND010000267         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEND010000267 DACEND010000000
VERSION     DACEND010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.284"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW746_002715"
     CDS             <1..1076
                     /locus_tag="IW746_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1079584.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEND010000268         1179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEND010000268 DACEND010000000
VERSION     DACEND010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.285"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IW746_002716"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IW746_002716"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1079585.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          335 a          245 c          179 g          420 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactac
//
LOCUS       DACEND010000269         1179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEND010000269 DACEND010000000
VERSION     DACEND010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.286"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(627..959)
                     /locus_tag="IW746_002717"
     CDS             complement(627..959)
                     /locus_tag="IW746_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1079586.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYVGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          346 a          251 c          161 g          421 t
ORIGIN      
        1 ttttcatttg tcattcctcc attacgtaaa tttcattttc tctatgtttt atatcaattt
       61 tttcacttta ttcaggactc gttcatattt tatcgttttt cgtttactta aaaatacata
      121 aaaaatatcg actcgtttat ggcgtccgaa acgagaaacc ctagtagaac atcggtaaat
      181 gctcgattga tccaataatc aacgtcctcg caagtatcta catgtactgc agcacacgac
      241 acaatttctt gtgcgtcctc cccattacga gaagacctac tccttattcc atgagtttgc
      301 gtcaaagcct agatccctta gtagctgctc atgattaatc taggcgctat tctaacttgt
      361 ctaacttcta aattccctct tgtttactct gtgcatatta tttacacaaa ttcatttcac
      421 cactatccaa acaagggcat ttactcgatt gaccaatttt ttacgttacc aatcgtaaaa
      481 acctaccccg tcaccccccg aaaggaattg ttgatagatc aagtctactc cttatttttc
      541 tagtaattct aagttctttt agaagtctcg acaatatttt caagcaagcg aaaaacgata
      601 aaatacaagt tataagaatg atcaattcag ctgatcaaac gccccatccc ttttgcggta
      661 caacgtaaga ctccattttt tcttcgacct atcaatacac cgttttggtc ataaacatgt
      721 ttgattgttg gatatagctc ttttaaaggg ataggatctt ttaaaattat ttgcttttgt
      781 ttcccaacgt aataaatcat ttcctctgag actttgtctt tttggattat atatttctta
      841 cactgcacaa cggttttatt ttggccagtt gatagtttct tcgtgatctt ctctgtagcg
      901 gtacaccgtt taccgtagtg acaaatatct tcgagactca aaattcctgt atatgacata
      961 aaataatcca cccacttact ttaaatattt ttaataagtt tttcttgtta tttttcaaaa
     1021 atagagaatt gattttcgtt catgacaaac aaaaaagagg gaacgcatcc aaacatgcgt
     1081 tccctctaat ttcagcgttc atcatacggc aaagagccac acggacattt ttataacatt
     1141 atgcttaggc atagattcat cgtaatatca cgtattttt
//
LOCUS       DACEND010000270         1178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEND010000270 DACEND010000000
VERSION     DACEND010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.287"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..190)
                     /locus_tag="IW746_002718"
     CDS             complement(<1..190)
                     /locus_tag="IW746_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1079587.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRN"
     gene            282..1142
                     /locus_tag="IW746_002719"
     CDS             282..1142
                     /locus_tag="IW746_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1079588.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          385 a          218 c          203 g          372 t
ORIGIN      
        1 gattccgata aatcgtatca taacttaccc ctttgaattc ctgattcata tagtcaaaaa
       61 cttctttagc agatacgtag cggttacgct tcactaaata agttaacaat gcttctcttt
      121 ttttagtata tttaaaaccg ttatttttca atgtttctaa tgattgttcg atcaatgtct
      181 gttttttcat gacttattcc tccaaatcaa aatgattacg aagtacgcat tctagagtat
      241 ggtataataa acataaaaaa aatgcaagtg aggttttatc catgtcttcc gattctacag
      301 aaatcgttac tttttttgtc atctccgact ctgctggaga aactgcgaca aaactagcgc
      361 aagctactat ggctcagtat cctaccgttg agttcaacct attccgcaga acgttcgtga
      421 cagatgaaaa aacattaaag caggcattgc aagatgcatt agaagaaaaa gcacttgtcc
      481 tgcatacatt aatcaatcaa gaactgatcg agataacacg ttctttttgt gaagaacatc
      541 aattatttag ttttgatgtg atgacgccac cgatatcaga aattgaacgt ttaaccggta
      601 tcaagccaat tcggcaacct ggtgccctcc atttattgaa tgagaactat ttcaaacgga
      661 tcaaagcaat ggaatttgct gtaaaatacg atgatgggaa agatcctcgt ggttttttag
      721 aagcagatgt cgttttgcta ggcgtatccc gaacttccaa aacgcctttg agcttgtttt
      781 tagctaacaa aaatttaaaa gttgccaatt tacctttgat cccacaagca catattccca
      841 aacagctttg ggagatcgat cctaaaaaaa tcgtgggact aacgaataac ccagatattt
      901 taaataatat ccgtaaagaa cggatgcgct cttacggatt aaaccctgat actgcttatt
      961 ccgatatcga aaaaattaga gctgaactcg attttgcgaa tgatctttat gaaaaactag
     1021 gatgtgtagt gatcaatgtg gcatctttat ctattgaaga aacggcttcg cttattttga
     1081 atgctttaga cctagaagat catagttatt atggaactga aacaacagaa aaaagcgatt
     1141 aatcattttt gtctttttct taaaaaacac tggagtgt
//
LOCUS       DACEND010000271         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.288, whole genome shotgun sequence.
ACCESSION   DACEND010000271 DACEND010000000
VERSION     DACEND010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.288"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            13..1098
                     /gene="xerS"
                     /locus_tag="IW746_002720"
     CDS             13..1098
                     /gene="xerS"
                     /locus_tag="IW746_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1079589.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          475 a          194 c          184 g          323 t
ORIGIN      
        1 aggatgaata acatgaaaaa tacagaaata tatcaaaaaa tagatacaat catacttcat
       61 atgcccgatt atataaaaga ttttgttcaa gatcgtgagg ataaagatca atcacctcga
      121 actactttag aatatctaaa aaattataag ctcttttatg aatggcttct gagtgagtct
      181 atcgttcccg atacttcaat cacctcgata agacagttaa ctgcctatga tctaaacttg
      241 tacaaatctc atttgaaacg tcgcacaaag gaaaatgtaa aaaaagagac aacaaaaact
      301 aagctcaaag ataataataa cttaggtcta tctacttcta ctatcaatag aaatatcaca
      361 gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt aggaaaacca
      421 tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac tacgttagct
      481 gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca aaattatcta
      541 gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat ctattatcaa
      601 cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg attaagactc
      661 tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa tgtggtagta
      721 actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat ggaatattta
      781 gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga aaaagcttta
      841 tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat cgaacggatg
      901 gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt acgtcatact
      961 ttggctacta gactctatca acagacaaat tcattggtat taactgccca acaattggga
     1021 catacaagta caaatacaac aactttatat actcatatag acaatgcagc tacaattgat
     1081 gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct gtctctaaaa
     1141 ttagataaaa gcccaacttt aaaggcaagc ttcatt
//
LOCUS       DACEND010000272         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEND010000272 DACEND010000000
VERSION     DACEND010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.289"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..>1173
                     /gene="gndA"
                     /locus_tag="IW746_002721"
     CDS             88..>1173
                     /gene="gndA"
                     /locus_tag="IW746_002721"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1079590.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKI"
BASE COUNT          394 a          206 c          268 g          305 t
ORIGIN      
        1 atatgttgtt ctttagtttg caatgtagcc gtgttaagct ataatgtaag agattaggga
       61 gaaatcccgt atgtaggagg agttcatatg tcaaaacaac aaatcggcgt tgtcggtatg
      121 gccgttatgg gtaaaaatct agccttgaac atagaaagcc gcggctattc agtggcttta
      181 ttcaatcgta caggggctaa aacaaccgct gtagtagaag aacacccaga taaaaatttc
      241 aaagcaacat atacaatcga agaatttgta gaatctatcg agaaaccacg tcggatcttg
      301 ctaatggtca aagcaggacc ggcaacagat gcaacaatcc aagagttact tcctcacctt
      361 gataaaggcg atattttgat tgacggagga aatacattct ttaaagatac gatgcgtcgt
      421 aatgaagagt tagccaactc tggcatcaac tttatcggta caggcgtttc cggtggtgaa
      481 gaaggagctt taaaaggacc ttcaatcatg cctggtggac aaaaagaagc ctatgcgcta
      541 gttgcaccaa tcttagagca aatttctgca aaagcagaag acggagcacc atgtgtcaca
      601 tatattggcc caaatggagc cggacattat gtgaaaatgg tacacaacgg tattgagtat
      661 ggagatatgc aactgattgc tgaatcttat gacttaatga agaatatttt aaacctttca
      721 gtagaagaaa tggctgatat ctttaaagaa tggaatcaag gcgaattaga cagctatttg
      781 atagaaatca ctgctgacat tctgacacgt aaagacgatg aaggaacagg taaaccagta
      841 gtagatgtga tccttgatgc agctgggaat aaaggaacag gtaaatggac aagccaaagc
      901 gcgcttgact taggtgtgcc acttccattg atcactgaat ctgtatttgc ccgctatatt
      961 tctgcttata aagaagaacg cgtacaagca agcaagattt tgtctcgtac gaacgatttt
     1021 gagttcacgg gagacaaaaa agaattggtc gaaaaaatcc gagaagcgtt gtatttcagt
     1081 aaaatcatga gttatgccca aggatttgct caattacgtg tcgcttcaaa agaatttgat
     1141 tgggacttgc catttggtga aatcgcaaaa atc
//
LOCUS       DACEND010000273         1171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEND010000273 DACEND010000000
VERSION     DACEND010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.290"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..61)
                     /locus_tag="IW746_002722"
     CDS             complement(<1..61)
                     /locus_tag="IW746_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1079591.1"
                     /translation="MVAKKYVIGNFKGGVGKSTC"
     gene            complement(347..634)
                     /locus_tag="IW746_002723"
     CDS             complement(347..634)
                     /locus_tag="IW746_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1079592.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(624..953)
                     /locus_tag="IW746_002724"
     CDS             complement(624..953)
                     /locus_tag="IW746_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1079593.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1030..>1171)
                     /locus_tag="IW746_002725"
     CDS             complement(1030..>1171)
                     /locus_tag="IW746_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1079594.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDEHVVEIYSARTHYE
                     SQ"
BASE COUNT          378 a          224 c          135 g          434 t
ORIGIN      
        1 cgcacgtact tttacctacg ccacctttaa aattaccgat tacatatttc ttcgctacca
       61 cgttcctacc tcttttcttt tagcaatatt catttgatac tattataaca tatttatttt
      121 tagttactac caaaaaaaag atatatatgt actaccttat aattaataag taaaaggtag
      181 tacataaata aggaaaagct gaaatatatt gatttatagc tttcctcaaa gtaatgacgt
      241 tatttaattt atcgagaagc agagaatact cgcgacttta gtcatgtaga cgaatcagat
      301 tttcgataaa aaagaagccg tccttcttaa gaacgacaac aaatcactag attaattttt
      361 tcaactgttc ataaaagttt tcatgcgttc cacataacaa tactggaata accttgtttt
      421 ctataatctc gtaagctact ctatatgttg tgcccgcata tttgaaccca ctagaccaaa
      481 tacctgataa atccccagtt ttttgttcac ccttatatgg atcaatcgca atttcatcat
      541 aaattaaatt caaaaatttt tctttcaatg gcttctcttt tagctttttt aaatacttaa
      601 tgacatgttt tgaaggaacg atttcataag ccaatttctg ttgccaactc ctctttagtc
      661 attactttag aattagtaag tgtatcttca gcaatatctc taaaggaaga atgcattttg
      721 ttttttctat tcttaaattc tacaagaatc tcatctttat cgtagccttc tgcaattata
      781 tcagacaaaa tatcttcgct aaaatcataa aattcttttt gtggttcaac aaattcataa
      841 agaataccat tctctaccaa ctttgcttca acttcgactc cattaggaac attaaaatct
      901 ttaggtactg tcaacatgat tgaattgcct tgttttcttg tttttgtaat catttaaatc
      961 cctcatttct aacagtatgt aattacttag taaatacatt ataccacgag ataatgtatt
     1021 tacaagtaat tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc
     1081 atcttcatct accttataaa caacccggtg ttgtctattc aatcttctaa aatacctacc
     1141 ttcatgtgta ggttttaact tctcaaatga t
//
LOCUS       DACEND010000274         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEND010000274 DACEND010000000
VERSION     DACEND010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.291"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            27..899
                     /locus_tag="IW746_002726"
     CDS             27..899
                     /locus_tag="IW746_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1079595.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          334 a          222 c          279 g          322 t
ORIGIN      
        1 attctaaaat aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa
       61 aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga
      121 caacgacacc tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag
      181 atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca
      241 cttatgggta cattacctcg accccgaaat tggcatggca aaattttgat ttcatcggta
      301 ccatgaaaaa agaatttccg atcccgattt cttggacaac agatgtcaat gcggctgcct
      361 atggtgagta tgtcttcggc agtggcaaag gattgagcag tgtcgtttac tatacgatcg
      421 gtacgggtgt tggcggcggt gccttacaag aagggcgctt tatcgaaggg tttagtcatc
      481 cagaaatggg gcatatgctc gtcgtccctc atccaaaaga tgactttgct ggcagctgtc
      541 catttcatgg caactgttta gaagggatgg ccgctggacc cgccatcgaa aaccgcttag
      601 ggaaaaaagg acaagaggtt gcagaagatg atccttattg ggaaatcgaa gcctcctata
      661 ttgctcaatg tgcctacaat acaaccttga tgttttctcc agatgtgatc atttttggcg
      721 gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca cgacgcattt gcaaaattgg
      781 tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca gcattagaag
      841 acaacgctgg aacgctcggt tgtttggcat tggctcgaga agcggtctta cattcataaa
      901 cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag atacaactga aaatttagtc
      961 ataaccagtt aggcagagtg cttaactggt ttttgtttta cttggaagat caagccttga
     1021 atgagataaa ttcttgctcg aaagttatgg aaatttcgat agccgtaggc cacttgtatt
     1081 gtgcaatagt tttttacaaa aaaaaggggg tgcggatatt tttttggacc tttttaacaa
     1141 atatatcttt gcttctt
//
LOCUS       DACEND010000275         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.292, whole genome shotgun sequence.
ACCESSION   DACEND010000275 DACEND010000000
VERSION     DACEND010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.292"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(401..601)
                     /locus_tag="IW746_002727"
     CDS             complement(401..601)
                     /locus_tag="IW746_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1079596.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          371 a          208 c          167 g          410 t
ORIGIN      
        1 cgtttttata tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt
       61 tgaactgtcc gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt
      121 ttactttttc attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg
      181 attggtcaaa ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt
      241 tatcaacatc actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt
      301 gttttataca ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac
      361 cgtcttttct ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg
      421 ttgcttgagg gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta
      481 aagttttgaa gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt
      541 caccagtgat aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca
      601 taaataaatt cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat
      661 aggcaaaagg agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta
      721 ctcatttaat atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat
      781 tcattgtctt ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact
      841 ttttgttttc ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta
      901 tgaaaacctt tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt
      961 gcctttcagc aagtatttaa agaaaggaga atggctatga cattctataa caaagatata
     1021 caatatcgag taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat
     1081 accaatcatg tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct
     1141 gcataaggag tggaaa
//
LOCUS       DACEND010000276         1150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEND010000276 DACEND010000000
VERSION     DACEND010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.293"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..684)
                     /locus_tag="IW746_002728"
     CDS             complement(<1..684)
                     /locus_tag="IW746_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1079597.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPF"
     gene            complement(808..993)
                     /locus_tag="IW746_002729"
     CDS             complement(808..993)
                     /locus_tag="IW746_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079598.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
BASE COUNT          336 a          295 c          204 g          315 t
ORIGIN      
        1 aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa
       61 ccgttgtgca caatggtgtc ctgaacgtac agcgacacct tccatatcca gagcagtcgc
      121 cacatcacgg ggatggatgc catccaaatt aaaagcaacc acgccagtat gatgttcagg
      181 atcttgcggt ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta aaacgtaggc
      241 aaccagttct tgctcataac gatgaatatt ctccatacca atctcattca agtaatcgat
      301 tgctcctgca atgttcggag taccagcttc aaatttccat ggcagctctt tccatgaact
      361 atcgtaaaga ttgacaaaat ctatcatttc accgccgaat tcgactggct ccatttgttc
      421 taacaattga cgtttgccat ataatacacc gattcctgtc ggcccgcaca ttttatgccc
      481 gctaaaggcg aaaaaatctg catccaacgc ttgtacatca actgtcatat gcggtgctgc
      541 ttgggcaccg actggactat cctcaactac aacagcctgt tcaaccctca gccgtttcaa
      601 gcacaacaaa taaccttctg gatctggttt gccgacctga agatcatcga tcgtcacaaa
      661 ggaatcaatt ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag
      721 ttcagaattg agactactcc gattttccag tgctgctgat ccgctgctca aaatgttcag
      781 catccactgg tctcccaacc gcttgactta tcccttcata aaaggacagt tctgctgccg
      841 tcccaacacc ttgttgatag tctgcaaaag gaattttata gccgaactgc tccagtaaat
      901 aggcttcagt aaatagtgat gaatactctc gtaaaacatc cattcactgt caatcatcgt
      961 cccatcaaaa tcaaaaataa gtgctttggt catcttcttt cccccttacc caaaacaagc
     1021 aaaagctgtg tcctgctctc tctacagtat cagcataaaa actgccgtct gaaaagtcaa
     1081 aggtgaaaga aatgtggcgc tataatttct aggactgata gtaaatcatt ctatcagtcc
     1141 ttttgagcat
//
LOCUS       DACEND010000277         1147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEND010000277 DACEND010000000
VERSION     DACEND010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.294"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(347..>1147)
                     /locus_tag="IW746_002730"
     CDS             complement(347..>1147)
                     /locus_tag="IW746_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1079599.1"
                     /translation="HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLK
                     RQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITD
                     GLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEAN
                     TFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYS
                     RRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          346 a          182 c          213 g          406 t
ORIGIN      
        1 gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga aaatggcttc
       61 tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac accgtttatt
      121 gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag ctccatatac
      181 tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca tttcttttat
      241 aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaaaa acttgggagc
      301 gtcaataatt ttgtgtaaat aaattgtcct cctgcaaaat aattagttac tcagtaaaca
      361 ttgaaactaa tgtatcggtt acctgttgaa aacctttatg gcttctgttt agaaattttt
      421 gattgtatgt atcaaaaatg ctgactagaa agcgttctag tgattcttca ttttgaaact
      481 gctcttttct acggctgtat cttttaattt gcttattgaa agactcgatt agattggttg
      541 agtaaatggt tctacgaatg ctaggtggaa aatcataaaa agttaataag tcttggtttt
      601 ctatgagtga ctgcgtcact ttaggatagt ttttcttcca tttctcaatc atgccggata
      661 agaaggtatt cgcttcttct tttgagttag cttgataaac agccttaaag tcatcacaga
      721 tttcttttcg gtctttgaca cgtactttat gagcgatgtt acgagataca tggatacaac
      781 aatgctgata ttttgcttta ggataaattt gatggatagt atctttcatg ccttttaagc
      841 cgtccgtaat aaaaagcaag acttcttgaa ctcctctgga gttaatatcc tgtagcagct
      901 cattccaaac gtatgttgat tcagttggag caatcgcata actcagtact tctttagtgc
      961 cgtcttctcg tataccaatg gcaatataaa tcgcttcttt ggatacggtt tgacgtttta
     1021 gtggaatgta agtagcgtcc ataaaaatag cgacatactt atcatttaag gctctggatt
     1081 taaaggcatt tacttcttca gtcagaactt tagtcatgtt ggacatggtt tgtggagtat
     1141 agtgatg
//
LOCUS       DACEND010000278         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEND010000278 DACEND010000000
VERSION     DACEND010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.295"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            43..249
                     /locus_tag="IW746_002731"
     CDS             43..249
                     /locus_tag="IW746_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079600.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(611..850)
                     /locus_tag="IW746_002732"
     CDS             complement(611..850)
                     /locus_tag="IW746_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079601.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          394 a          162 c          210 g          366 t
ORIGIN      
        1 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtggtt
       61 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
      121 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
      181 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
      241 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
      301 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
      361 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
      421 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
      481 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
      541 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
      601 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
      661 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
      721 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
      781 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
      841 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
      901 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
      961 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
     1021 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
     1081 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat ag
//
LOCUS       DACEND010000279         1094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEND010000279 DACEND010000000
VERSION     DACEND010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.297"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1054
                     /locus_tag="IW746_002733"
     CDS             <1..1054
                     /locus_tag="IW746_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1079602.1"
                     /translation="ELAYISDPGHIAYFSGYESDPHERVLALFIPLEQDPFLFTPALE
                     VEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNVGIEKNALTVERFDAISDI
                     LTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAFEIGFKAVKEGVTEQAIIA
                     EIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISPNELVLFDLGVVWNGYCSD
                     ATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGELDQIARNVINSYGYGEYF
                     NHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYIPEKVGVRIEDCVYVTSDG
                     CVPFTTTPKELLVLEK"
BASE COUNT          330 a          239 c          240 g          285 t
ORIGIN      
        1 cgaattggct tatatcagcg accctggcca catcgcttac ttttccggat acgaaagtga
       61 tcctcatgag cgtgtactag ctctttttat ccctcttgaa caagatcctt tccttttcac
      121 tcctgctttg gaagttgaag atgctcaaaa cagtagttgg gcgtatgatg tccgcggcta
      181 tttagacagc gaaaaccctt gggagatcat cggaaaagaa cttcgtactc gctatggtca
      241 gcctggcaat gtagggattg aaaagaacgc cttgactgtc gagcgcttcg atgctatctc
      301 tgatatcttg acgggaacgg attcttacac tgatctaact tccatcatcc aacgtctaca
      361 actgatcaaa actctagagg aaaaagacaa actgatcgaa gctggtaaat gggccgatgt
      421 tgcatttgag attggcttta aagcagtgaa agaaggcgtg actgaacaag cgatcattgc
      481 agaaattgaa taccaattga aaaaacaagg tgtttcacaa atgtctttcg acacattagt
      541 ccttgcagga gcaaatgctg caagccctca tggtacacca ggaaatacga agatttcacc
      601 gaatgaactt gtgttattcg atcttggcgt tgtttggaat ggctattgca gtgatgctac
      661 tcgtacaatt gcttatcaaa aaccaaccga cttccaagaa aaaatctaca acatcactct
      721 tgaagcacag cttgccgcac aagaagccgt ccgtccaggt gtgacagcag gtgaacttga
      781 tcaaatcgca cgaaacgtga tcaacagcta tggctacggc gaatatttca atcaccgttt
      841 aggtcacggg atcggaacaa ccgtccacga attcccttcc cttgtagaag gaaatgactt
      901 agtaatcgaa gaagggatgt gcttctctct tgaaccagga atctatattc ctgaaaaagt
      961 aggggtacgt atcgaagact gtgtatatgt gacaagcgat ggctgtgtgc cattcacgac
     1021 tacaccaaaa gaattacttg ttttagaaaa ataagagtaa aaaaggtgag agctagtccg
     1081 ctcccacctt ttta
//
LOCUS       DACEND010000280         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEND010000280 DACEND010000000
VERSION     DACEND010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.298"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..417
                     /locus_tag="IW746_002734"
     CDS             7..417
                     /locus_tag="IW746_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1079603.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            431..787
                     /locus_tag="IW746_002735"
     CDS             431..787
                     /locus_tag="IW746_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1079604.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          378 a          162 c          207 g          335 t
ORIGIN      
        1 gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa agcaagagca
       61 tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga accgttaaca
      121 gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga tattatttcc
      181 actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac tttaaagcaa
      241 atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt gattgatgaa
      301 aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc tcgtgaaatt
      361 cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa ttcataggga
      421 gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc
      481 gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag aagaacgcga
      541 tctaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag cagaacagct
      601 atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct gtgtaggaaa
      661 aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc ctttgattat
      721 tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc ttatcttaaa
      781 taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt gtaacagtcg
      841 aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc tactatagag
      901 acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag ttaaaaatat
      961 attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta gcaaagcaat
     1021 aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat gacatgataa
     1081 ga
//
LOCUS       DACEND010000281         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEND010000281 DACEND010000000
VERSION     DACEND010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.299"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..902)
                     /locus_tag="IW746_002736"
     CDS             complement(24..902)
                     /locus_tag="IW746_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079605.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          338 a          205 c          170 g          361 t
ORIGIN      
        1 taaactattt cgaagtacta aacttatgaa gtaagaatac ctaagccaga gccaatgtgc
       61 tcgcctgcaa gcaattgctc ttttgcttgt cgctgtaaac tcgtactttc tggataatca
      121 tgtatttggg catctactcg tccaaagatc caagggactc cactatcgtc ctgttcatca
      181 actgataata atcgataatc ggctgcaaaa agacagattt ttgactcttt tttcaaccat
      241 ttagtaagat tgggtgatag taaccaagta tctgtgtaca tcacttggta atggtaatca
      301 ggaaaataaa gttcaaaaaa acgaatggca ctttcgtaac tatctcttat cttattctga
      361 gataaatctg catcggaagg aatatgaaga gaaaggcact gctttccttc taatcttaga
      421 tccgtgtacc ctttcggaaa atatgtcatt tcgtactcca attcaccaat ccgaaagatc
      481 aagccactag tgtaatgcca aatccaaaaa ccacggtcaa agcgatagct tcctgttact
      541 tggaaatgtt catgaataaa tctagtaaaa gcattcattg ttgccaaata gatagttctt
      601 ggaatcttca atggttgata aatttttttc caactttgag aagctgcata tagataaata
      661 gctaatgctg caaaaggttc ctcttcatag actttgtcta ttttctttgc agtcgccttt
      721 gctttattag gatccattag ttcatgacta tattgctcaa acacctcatc aaggatgttt
      781 tttgcttgta caatcatttg ctctataacg actttgggaa gagttaaagt ttctgccaga
      841 atttggattt cattgcttat tttcttcatc aaatttcccc ttttcatatc tttgaacctc
      901 attgtttaaa tgactgatta tccggacacc atatggtata aacatacttt tattgtacac
      961 taactataaa acttaaactt tctatgaaga aagaatatct gtaagacttt caaggaaagc
     1021 attgaccatc tcaggaaagt ttattcagtt accatcataa acaaaatttt ttga
//
LOCUS       DACEND010000282         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.301, whole genome shotgun sequence.
ACCESSION   DACEND010000282 DACEND010000000
VERSION     DACEND010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.301"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>1073
                     /locus_tag="IW746_002737"
     rRNA            2..>1073
                     /locus_tag="IW746_002737"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACEND010000283         1030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEND010000283 DACEND010000000
VERSION     DACEND010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.304"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..494)
                     /gene="mreD"
                     /locus_tag="IW746_002738"
     CDS             complement(<1..494)
                     /gene="mreD"
                     /locus_tag="IW746_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1079606.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
     gene            complement(498..>1030)
                     /gene="mreC"
                     /locus_tag="IW746_002739"
     CDS             complement(498..>1030)
                     /gene="mreC"
                     /locus_tag="IW746_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1079607.1"
                     /translation="ELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANM
                     AVMSQKGLIGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLH
                     ALVVTQLTGDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAE
                     MYDVSVVTIVQRLAED"
BASE COUNT          330 a          235 c          173 g          292 t
ORIGIN      
        1 aatttcttaa agggaaaaat gaataataca aatataatca tgttcaacag cagtgtaggt
       61 cctaaaaacc ttgtaataaa atacgtgtta tttactactg ctaatttaaa aatcaactgg
      121 accaccatcg taaacaattc ataacccgtg acaaagatga tcatactgaa aaattcagta
      181 aagatgttga catggataac gtctttcatt acatacatca accatacaat taaagggaga
      241 gctactgcat agataccgat aacgccaata tagtaagcgt catagatagc cccgagaaca
      301 atcgtggtaa tcagtaaata acgtttttca aacgccatac tgcaacataa taatgccaat
      361 atcagaaagt gggcatttga catataggtg cctttcgacc actcccctag cattcttgtt
      421 aaatgaccat ctatcaacat caaaaagaac aaaacgattg gcagatagta cttcattgtt
      481 tctttcttca acatggacta atcctccgct agccgttgga caatcgtcac aactgacacg
      541 tcatacattt ctgcataagg tttcacataa acttcccgat ccagcccata actatctggt
      601 ttcgttttaa taaccgtacc gatcggcaag ttagctggag aattccctcc aagaccggat
      661 gtctggacaa catccccttc tttgatatcc gtatcaccag ttaattgggt caccactaag
      721 gcatggagct tttcatcata gtttttaagc aaaccaaacg cttcgccatt agccgaagat
      781 acccgtactg gaaaatgatt ggagctttca ttagaggatg acagtaattc gattttagac
      841 gaagccgtat tgacctcgat cactcggcca atcaatcctt tttgcgacat gactgccata
      901 tttgcttcga taccgtcttt agaacccttg tctacgatca gcatgtcctg ccaactgtca
      961 ggcgaacgag taatgacatt tgccgttact ttttcgtaac tagtcagcgt ctgattcaac
     1021 tcaagttctt
//
LOCUS       DACEND010000284          996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEND010000284 DACEND010000000
VERSION     DACEND010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.305"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..549
                     /locus_tag="IW746_002740"
                     /pseudo
     CDS             70..549
                     /locus_tag="IW746_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT          325 a          173 c          229 g          269 t
ORIGIN      
        1 ggcttattac cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt
       61 gtggcacgat tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg
      121 gtaaccccat tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata
      181 ctcaatcgtc aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc
      241 acagagggat gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga
      301 gtcggtggga cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg
      361 gcatcgggaa tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg
      421 ttagctggtt cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg
      481 aatgcagaaa tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca
      541 attagttagg agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac
      601 aggaatggta tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga
      661 gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga
      721 gaacctttcg ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa
      781 actttcctgt actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat
      841 tcttcatgct agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg
      901 cgcaacaggt gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt
      961 tacaaatcca gctatttatc gttaacaaaa agattt
//
LOCUS       DACEND010000285          993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEND010000285 DACEND010000000
VERSION     DACEND010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.307"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>993)
                     /locus_tag="IW746_002741"
     rRNA            complement(<1..>993)
                     /locus_tag="IW746_002741"
                     /product="16S ribosomal RNA"
BASE COUNT          207 a          317 c          220 g          249 t
ORIGIN      
        1 cgtcatcccc accttcctcc ggtttgtcac cggcagtctt gctagagtgc ccaactgaat
       61 gatggcaact aacaataagg gttgcgctcg ttgcgggact taacccaaca tctcacgaca
      121 cgagctgacg acaaccatgc accacctgtc actttgcccc cgaaggggaa gctctatctc
      181 tagagtggtc aaaggatgtc aagacctggt aaggttcttc gcgttgcttc gaattaaacc
      241 acatgctcca ccgcttgtgc gggcccccgt caattccttt gagtttcaac cttgcggtcg
      301 tactccccag gcggagtgct taatgcgtta gctgcagcac tgaagggcgg aaaccctcca
      361 acacttagca ctcatcgttt acggcgtgga ctaccagggt atctaatcct gtttgctccc
      421 cacgctttcg agcctcagcg tcagttacag accagagagc cgccttcgcc actggtgttc
      481 ctccatatat ctacgcattt caccgctaca catggaattc cactctcctc ttctgcactc
      541 aagtctccca gtttccaatg accctccccg gttgagccgg gggctttcac atcagactta
      601 agaaaccgcc tgcgctcgct ttacgcccaa taaatccgga caacgcttgc cacctacgta
      661 ttaccgcggc tgctggcacg tagttagccg tggctttctg gttagatacc gtcaagggat
      721 gaacagttac tctcatcctt gttcttctct aacaacagag ttttacgatc cgaaaacctt
      781 cttcactcac gcggcgttgc tcggtcagac tttcgtccat tgccgaagat tccctactgc
      841 tgcctcccgt aggagtttgg gccgtgtctc agtcccaatg tggccgatca ccctctcagg
      901 tcggctatgc atcgtggcct tggtgagccg ttacctcacc aactagctaa tgcaccgcgg
      961 gtccatccat cagcgacacc cgaaagcgcc ttt
//
LOCUS       DACEND010000286          965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.309, whole genome shotgun sequence.
ACCESSION   DACEND010000286 DACEND010000000
VERSION     DACEND010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.309"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            455..634
                     /locus_tag="IW746_002742"
     CDS             455..634
                     /locus_tag="IW746_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079608.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT          324 a          143 c          190 g          308 t
ORIGIN      
        1 aaatgaataa ggtgatttat caactaacaa cggattccat atcaacgatt tgaaagaaaa
       61 aaaggtcaat aatcatttat accgaaaagt gagaatgtgt gaaacaagga caaaaaaagc
      121 ctagtaacta aggaaatggt tcttatttcg caccaatcgc tccaaccgcc cccgccgctt
      181 aggtggtgcg aaataaacgg gcaaagcccg ataacttaag agaaagaagg tgatccattt
      241 gaacaagatt attcccttat tgttagtact gtttgttggc gaaatactta tgcgagatag
      301 tgcagctgta cagttaaacc tcgctgtatt tttggtaata ctggtgtttt gtttgatctt
      361 ttggcggttt gtattagtcc tcgtgagtct actattcaag atgattgcag ccatattttt
      421 catagggtta tttcgatgaa aggagtgagt accaatgcca attgcaatag gaaataaacg
      481 gttacccgtg acattagatg aaaagagaca aaaagaattg cagcaactaa agcagaagta
      541 tggcaaaagt gaatctaaga ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa
      601 agcaggattt gatgtaccag cgctaaaaaa gtgacgtcac tttttaattt gatctatata
      661 agtgatatca cttttaccaa ggagggaatt aaatgttaaa aactaaaaag aattcgtaca
      721 aattaaagct tagtatacta tctattattt gtcttttagg catgataatt agtggtcatt
      781 tgtatagagt ggcatatacg cagtttattg agtacaaggt tcactcttta ggattagctg
      841 gggatcgata cgttgaatat gtccattatt cattaaaatg ttcttcatat attcttcttt
      901 ggcttatttt ttcattgttt gaattgctaa atttaatagc aatatcttca tatcacttag
      961 ataag
//
LOCUS       DACEND010000287          917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.312, whole genome shotgun sequence.
ACCESSION   DACEND010000287 DACEND010000000
VERSION     DACEND010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.312"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..769
                     /locus_tag="IW746_002743"
     CDS             <1..769
                     /locus_tag="IW746_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1079609.1"
                     /translation="HLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMT
                     DEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQH
                     TKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQ
                     VLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDA
                     IIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          326 a          173 c          154 g          264 t
ORIGIN      
        1 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
       61 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
      121 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
      181 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
      241 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
      301 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
      361 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
      421 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
      481 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
      541 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
      601 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
      661 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
      721 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
      781 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
      841 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
      901 acagaacaga aagaggt
//
LOCUS       DACEND010000288          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEND010000288 DACEND010000000
VERSION     DACEND010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.317"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            74..>900
                     /locus_tag="IW746_002744"
     CDS             74..>900
                     /locus_tag="IW746_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1079610.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQK"
BASE COUNT          301 a          168 c          178 g          253 t
ORIGIN      
        1 tattaaaaaa catatcgttc tctagtatga ttaaatcacc acaaaataaa caactgagag
       61 gacgatacgc tatatgaatg attctatcaa aaaaatgctg agaataacag agaaagattt
      121 gatgattaca gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc
      181 tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa
      241 cgggaaagca attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata
      301 caatcatatg cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg
      361 cacccattgg acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt
      421 tagatataaa attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag
      481 ctgtcaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc
      541 aaagcaatct aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc
      601 ttccatagaa gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga
      661 tgttttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc
      721 agaagaagta gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg
      781 tgttctgcca aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca
      841 agcgttcaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag
//
LOCUS       DACEND010000289          896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEND010000289 DACEND010000000
VERSION     DACEND010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.319"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(97..645)
                     /gene="aac(6')"
                     /locus_tag="IW746_002745"
     CDS             complement(97..645)
                     /gene="aac(6')"
                     /locus_tag="IW746_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1079611.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          262 a          185 c          132 g          317 t
ORIGIN      
        1 gaaataagtt ggaatttcac aaaaattcaa gaagtaattt tcgtaaattc cttcttattt
       61 cttgaagctg attacttctg tcacaacctc tttttattat tgagaatttg gtcgaggaat
      121 aatcgttttt gccatccaaa tatccggttt gtcccagcca tttgcatttg gtaagacacc
      181 tacgatttta taacctaatt tttcatagaa ttcatacgga tgttcacgaa ggttctggat
      241 ggaagccact ttatcaaatg tatgctcata caggtccgtt tgacttaacg ttgttccatg
      301 gtctaaatca tccgtaccta aataaatcgt gattcctcct ctggaagcta cttctttttc
      361 taagtaattg actaatcgag tacctatttg gttctttcgt cgggagcttt ctacaactaa
      421 tggatgcaat tcccaacctg tgataccgta ttgagggatt gcaccaataa atcctactaa
      481 ctcatcttgg tctaccgctg ctaccgcgat tcgttctgga ttcatcattt cttctacttc
      541 ttctgccgag ctgtctccat attcttccgg ccaagtcagt ctcagtaaat cagaaagctg
      601 atctttcaat actggattat tacggtcaaa ttcactgatt atcatacgtc ttcccccttg
      661 ttcttgctta ttctaacagt ccttttgaaa aaacgaaatg ttctcgctca aaaatcttta
      721 gctaccaatc agtatctcta ttctttcatt atttatctta cttataaaat aaaaaaatta
      781 tacagaaaca ctttattcaa aataggaaag atcaggaaag ccatcaattg gaggaaaaaa
      841 ctctgtttgt tcatcatgat atagattatc ttttgcttta aaaaaaacca attatg
//
LOCUS       DACEND010000290          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEND010000290 DACEND010000000
VERSION     DACEND010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.320"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..>887
                     /locus_tag="IW746_002746"
     CDS             6..>887
                     /locus_tag="IW746_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1079612.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQD"
BASE COUNT          281 a          177 c          170 g          259 t
ORIGIN      
        1 tctgtatgcc tgatcttcat catatcaaac tactacttgg aatcaaagat aaaaacattt
       61 tcatcacaaa tgtggaatcg aagagcatta agaatatcaa gagtcttgtt gtttctgcga
      121 cgttaagtaa agaaattcgt cgctgtcctt tgtgtaaaca gatgaatcat gaaggcatga
      181 tcgttaagaa cggaaagaaa aaatcgctca ttcaacttaa taaatgtgcg aaccaactga
      241 cttatcttgc tttagcgaaa caacgctacc actgtcgtgg ctgccacacc tattttacgg
      301 cgaataccta cattgtcgat agaaactgct ttatcgctaa acaagtgcgc tataaaattt
      361 tagaagagct aactgaaaaa caagcaatga cgacaattgc taagcattgc ggcgtttctt
      421 ggtcgaccgt ttctcggaca ctcgcttctc tgatccccat gacaaaagta aaaagaaatt
      481 ggttaccgcg ttgtttatta atggatgaat ttcgttcact gaaaaatcaa gtcggtccct
      541 actccttttc ttgtatggat ggcgatactg gaaaattact ggatattctt ccttcacgga
      601 agaaaaaggc tctcgtttct tattttatgc aatttgaacg acgcgcacga ttgaaggtaa
      661 aggtcatcgt gacggacatg aatgtttctt atgcctctct gataaaggaa tgttttccaa
      721 aggcaaaact cgttgtggat cgttttcaca ttgtcaaaca tctcattcgt aaatttgagg
      781 atattcgcgt acgaatcatg aaaagctttg atcgaaacga tccaacacaa gccaaatatt
      841 atcgacaatt aaaggcgttg tctcggcttc tgatcaaacg acaggac
//
LOCUS       DACEND010000291          867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.322, whole genome shotgun sequence.
ACCESSION   DACEND010000291 DACEND010000000
VERSION     DACEND010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.322"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..610
                     /locus_tag="IW746_002747"
     CDS             179..610
                     /locus_tag="IW746_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079613.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            612..749
                     /locus_tag="IW746_002748"
     CDS             612..749
                     /locus_tag="IW746_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1079614.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
BASE COUNT          328 a          124 c          177 g          238 t
ORIGIN      
        1 gaaggctatc ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact
       61 ttgaacagtg ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca
      121 agttatggat cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat
      181 gaaagtagtt tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca
      241 agaaaatatc cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag
      301 atttgatttt gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa
      361 tgctgttgat agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc
      421 agaaaatgat gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa
      481 gcaagaaaat gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac
      541 gatgcttgct tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg
      601 tgggaagtaa catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca
      661 ctaacgaaca agtaatggtt ttcgttccat tatgcattac agacgaagaa gcagataaaa
      721 tcattaataa agataagagc gcatcttaat tgatgcgttt ttatttaagg taaaggagtt
      781 gtcacatgat taatttaggg gaatggggaa tgatagcagg atcaataacc gctatcgttt
      841 ctttgatttt attagtaatt agaccga
//
LOCUS       DACEND010000292          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.323, whole genome shotgun sequence.
ACCESSION   DACEND010000292 DACEND010000000
VERSION     DACEND010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.323"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..424
                     /locus_tag="IW746_002749"
     CDS             149..424
                     /locus_tag="IW746_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase"
                     /protein_id="HAQ1079615.1"
                     /translation="MFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTKDH
                     ANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(538..>863)
                     /locus_tag="IW746_002750"
     CDS             complement(538..>863)
                     /locus_tag="IW746_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1079616.1"
                     /translation="DEGATVILDLDDGVGEYSKMGVCSLDTSFRLLLLDKEQRKNDYK
                     LQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKF
                     SGIKQ"
BASE COUNT          293 a          160 c          124 g          286 t
ORIGIN      
        1 atctttaggc gctgttattg ctttaaacta tcttgcaact ccttctccat atttaaaaaa
       61 ggttgtttta gtcgctccac aatttgagtc tcctaatcgc ttattgctta ctttccagaa
      121 tatttatttt tactgcgtac cttctaagat atttgaaaaa ttaggtctat ctaaaaaaca
      181 aacccttacc ctacttcgtt cacttgaaac attagactgc aaaaaacagc ttcgtacatc
      241 acgacttcct aaattgattt tatgcggtac caaagatcat gcgaataaac cagcagcaaa
      301 acgtttatat aaactttcaa cgaatggccg gctactcttt attaaaaacg gaaaacatga
      361 attgaataga gaaaagccag aaataatggc acaagctatg cgatctttta tcgaaaatag
      421 ttaatttaaa aaaagacgac aaaatgtgtc gtctttttat ttaccatggt aagaatgttg
      481 tcttgttttt tcaatacagc ttgattgaga ctagttttat ggacaataga gagaaagtta
      541 ttgttttatt ccagagaatt tttctggtcg tagatctact aattgaacat gactgtctaa
      601 agaaccactt gggctttcaa gagtaaatac acctagacgt ggatctaaag aaagggtcat
      661 atgttcatcc atgtacattt ttgaataatc ttttatataa acatccccaa tatcactatc
      721 taattggagt ttataatcat tttttctctg ttctttatca agaagaagta aacgaaaact
      781 agtatctaaa gagcaaacac ccattttaga atattcccca acgccatcat ctaaatccaa
      841 tataactgtt gcgccttcat caa
//
LOCUS       DACEND010000293          819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEND010000293 DACEND010000000
VERSION     DACEND010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.329"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            273..389
                     /locus_tag="IW746_002751"
     CDS             273..389
                     /locus_tag="IW746_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1079617.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          289 a          130 c          134 g          266 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
      481 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
      541 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
      601 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
      661 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
      721 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
      781 cgagcaatac ggttgcaaat aaccattatc gtttaataa
//
LOCUS       DACEND010000294          783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.333, whole genome shotgun sequence.
ACCESSION   DACEND010000294 DACEND010000000
VERSION     DACEND010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.333"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IW746_002752"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IW746_002752"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="IW746_002753"
     tRNA            207..279
                     /locus_tag="IW746_002753"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="IW746_002754"
     tRNA            283..355
                     /locus_tag="IW746_002754"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="IW746_002755"
     tRNA            363..444
                     /locus_tag="IW746_002755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="IW746_002756"
     tRNA            462..534
                     /locus_tag="IW746_002756"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="IW746_002757"
     tRNA            546..617
                     /locus_tag="IW746_002757"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="IW746_002758"
     tRNA            634..719
                     /locus_tag="IW746_002758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
BASE COUNT          194 a          178 c          216 g          195 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 ggg
//
LOCUS       DACEND010000295          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.334, whole genome shotgun sequence.
ACCESSION   DACEND010000295 DACEND010000000
VERSION     DACEND010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.334"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..731
                     /locus_tag="IW746_002759"
     CDS             75..731
                     /locus_tag="IW746_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1079618.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
BASE COUNT          269 a          112 c          158 g          238 t
ORIGIN      
        1 atacctttgg actttaaaaa gttttaaagt actgtatgat ctttttagat gaaagtttgg
       61 atgggggagc atcattggat agtagagtct taaaggaata tttggataaa aatgattttc
      121 cgattatacg caaaggatat cataaatatt tgacattcga agggatggaa gattcccata
      181 cgtatatctt gaaagaggga acggtaaaag caagtgttat ttcaaatgat gggagagaat
      241 ttaaccttcg ctatatcaaa gatcttgaaa tcgtctctct tttaaaagac gaatattcac
      301 aattcatcga ttcgccgtat aatattcgag ttgaatcaga atatgcagag ttttatcgaa
      361 tcaatcgtgt gcgcttttgg caaaatatca accaagaccc aaagctacaa cactatgtaa
      421 aagagtatta ccgctatcgt ctgctttatt cgatgaaaaa aatgcagcaa atgctattaa
      481 acggaaagct gggagctgtt tgtacgcaac tttacgaatt atatgattta ttcggtgtgc
      541 ggacagaaga agggatgctg atcgattttt cgatcacgaa tgaagaaatc gccaaatttt
      601 gtggcatctc aactgctagc agtgtaaaca gaattttaaa acaactgaaa aaagatggta
      661 ttatcaccgt taaagaccat catatcgtta ttttgaatat ggacttgtta gaagacaata
      721 ttatcttttg atgaacaaag aggttgtgaa atcagcgttt tgacctgagt attagag
//
LOCUS       DACEND010000296          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEND010000296 DACEND010000000
VERSION     DACEND010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.340"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..240)
                     /locus_tag="IW746_002760"
                     /pseudo
     CDS             complement(52..240)
                     /locus_tag="IW746_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            220..>734
                     /locus_tag="IW746_002761"
     CDS             220..>734
                     /locus_tag="IW746_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1079619.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT          255 a          114 c          162 g          203 t
ORIGIN      
        1 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
       61 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
      121 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
      181 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
      241 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
      301 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
      361 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
      421 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
      481 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
      541 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
      601 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
      661 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
      721 agcagaatga taga
//
LOCUS       DACEND010000297          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.341, whole genome shotgun sequence.
ACCESSION   DACEND010000297 DACEND010000000
VERSION     DACEND010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.341"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..731
                     /locus_tag="IW746_002762"
                     /pseudo
     CDS             55..731
                     /locus_tag="IW746_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          125 c          137 g          172 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt c
//
LOCUS       DACEND010000298          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEND010000298 DACEND010000000
VERSION     DACEND010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.343"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>697
                     /locus_tag="IW746_002763"
     CDS             <1..>697
                     /locus_tag="IW746_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ1079620.1"
                     /translation="NGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIR
                     YFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHM
                     IVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITI
                     SVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILL
                     AYSVLLSRAQRRF"
BASE COUNT          237 a          130 c          123 g          207 t
ORIGIN      
        1 ttaatggcta tactagccaa agagccacgt atagagcggt ttgttccgtc ttatttccta
       61 atggtgactt tcctagtagg agtcgattca ctcgcttaag ttcaaactta gcttacacta
      121 tcaagattat tcgatacttt ttcatcaaga agctcaccaa aggtgaacta gtcggaatta
      181 tagatagttt tcctagtcca ttatgtaaac cagttagaaa tagacaagca aaactattga
      241 atcaaatagc caaagttggc tataactcaa caaaaaaatc ttatttttat ggtcttaaaa
      301 ttcatatgat cgttactaag actggctttc cgattactta ctcaatcacg aatccaggtg
      361 tccatgacgt gaaagtgtta gagactttat cggaagaagc gaatcttccc aatatcctag
      421 gggacaaagg atatattagc cataaaatcc atgaaaagct tgcccttaag ggcattacta
      481 tatccgttcc gccacgtaaa aacatggata aatcagaaaa actagaccac agcttacttg
      541 gaaaacaaag aaaaacagtt gagactgttt tttcttcctt agaaaaatta ggttgtcaaa
      601 atttcaactc acgttctgtt aaagggctag agagtagatt tgaaagcata ttactggctt
      661 acagtgttct attaagtcga gcacaacgac gttttga
//
LOCUS       DACEND010000299          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.345, whole genome shotgun sequence.
ACCESSION   DACEND010000299 DACEND010000000
VERSION     DACEND010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.345"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..684
                     /locus_tag="IW746_002764"
                     /pseudo
     CDS             1..684
                     /locus_tag="IW746_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          251 a          123 c          145 g          165 t
ORIGIN      
        1 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
       61 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
      121 caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata
      181 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
      241 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
      301 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
      361 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
      421 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
      481 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
      541 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
      601 ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa tccagatcaa
      661 gagattgaag cggaaatgct taaa
//
LOCUS       DACEND010000300          683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEND010000300 DACEND010000000
VERSION     DACEND010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.347"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..397
                     /locus_tag="IW746_002765"
     CDS             <1..397
                     /locus_tag="IW746_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079621.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            397..>683
                     /locus_tag="IW746_002766"
     CDS             397..>683
                     /locus_tag="IW746_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079622.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNG"
BASE COUNT          240 a          109 c          160 g          174 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgtagaag ctattgatgg taaagaagca
      481 ttggtgcaga cagatggaga ccaagccatt gcaggacata agaatttttc aggttctgta
      541 acaatcaata ataaacgtgt tttgacgact gatgataaca gatatgaagt ggtaaatctg
      601 gttgtcacta acggtaatac agggacagca aagctttatc gtgaaggaaa aacagtttct
      661 atatattttg tggctttaaa cgg
//
LOCUS       DACEND010000301          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEND010000301 DACEND010000000
VERSION     DACEND010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.349"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..632)
                     /locus_tag="IW746_002767"
     CDS             complement(81..632)
                     /locus_tag="IW746_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1079623.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          194 a          125 c           91 g          258 t
ORIGIN      
        1 gagttacccc cttatttgca tttattttgc attttaaggg ggtattttgc gtatttagcc
       61 ctttttatat ataaatctac ctaaaggtca ttaaatactc taaaaaacaa tgctatgagc
      121 cacagaatga tagcgaagaa tacatagggt actgcataaa gtatcaacat caaatatcgc
      181 catgcacttt cgtggtcatc aataaaacca ttaataaagc tatataaatg ttcaatacca
      241 aagaaatgac ctatcggacc tgtcacaagc attacaagta caaaaataat tagcacaaaa
      301 aatatatttg taacgccttt tgtcatcact tttagcgctg tattcgattt atcaaccact
      361 ttgttatggt cttttatttt actttctaag atttctttat actcttttgt ttcttctctc
      421 gcttgtttcg tcacttcacg tatttcgtct ttagtatcgc ttaattcatc ttgaatagct
      481 tgttgaaaat ctaatttcaa attatcttca tcaagtcgtt taatatagtg ctttgcagtt
      541 gtttcaaagt ttaatcttgc atgcttagtc ttttcttcaa aaacatctaa cttattatta
      601 aagttcgagg tcgaagcctc tagtcttttc atgagtatgt ttgttctctc gttcctgtct
      661 tgctcgct
//
LOCUS       DACEND010000302          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEND010000302 DACEND010000000
VERSION     DACEND010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.353"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>652
                     /locus_tag="IW746_002768"
     CDS             <1..>652
                     /locus_tag="IW746_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1079624.1"
                     /translation="NKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCS
                     ARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGH
                     LEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSI
                     TFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          215 a          112 c          134 g          191 t
ORIGIN      
        1 aacaaatcaa actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt
       61 caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct
      121 attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct
      181 gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa
      241 actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt
      301 ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta
      361 gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat
      421 attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt caaatccatc
      481 acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt caatgatatt
      541 gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga gaattctaac
      601 ggattgttac gtagagatgg tttattgaaa tctatggatt tcaattcagt ag
//
LOCUS       DACEND010000303          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.370, whole genome shotgun sequence.
ACCESSION   DACEND010000303 DACEND010000000
VERSION     DACEND010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.370"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..522
                     /locus_tag="IW746_002769"
     CDS             <1..522
                     /locus_tag="IW746_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079625.1"
                     /translation="KMISGQSKVLSVNGKYGAVILTKADLGLENAITELPYASETSDG
                     ILTAEMFQKIVNGEGGTYILPIATPEQLGGIKVGELLEVTEEGVLSATKQTDFNFSEE
                     LKGKLESLKILKAGANISIAEDGTISSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNE
                     RIPNFTFEKIGEV"
BASE COUNT          218 a           72 c          116 g          150 t
ORIGIN      
        1 aaaatgatat caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt
       61 ttaacgaaag ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag
      121 acaagcgatg gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga
      181 acatatattt tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc
      241 ttagaagtta ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct
      301 gaagaattaa agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca
      361 attgcagaag atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag
      421 tcatatgtag atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa
      481 agaattccaa attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta
      541 aaaataaaac aaagcg
//
LOCUS       DACEND010000304          549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEND010000304 DACEND010000000
VERSION     DACEND010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.374"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..549
                     /locus_tag="IW746_002770"
                     /pseudo
     CDS             1..549
                     /locus_tag="IW746_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          201 a          112 c          116 g          120 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      241 tctcgtcaga cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      301 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      361 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      421 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac agcttgtaat
      481 gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc attggaagcc
      541 atcgttacg
//
LOCUS       DACEND010000305          547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.375, whole genome shotgun sequence.
ACCESSION   DACEND010000305 DACEND010000000
VERSION     DACEND010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.375"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..482
                     /locus_tag="IW746_002771"
     CDS             <1..482
                     /locus_tag="IW746_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079626.1"
                     /translation="AKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQ
                     DLIYYVHEDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVK
                     KLKDQLPYQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
BASE COUNT          239 a           83 c          109 g          116 t
ORIGIN      
        1 gagccaaagt gatcaaggtg tctgatgatt attttgcagt gaagctaacg catgtaccag
       61 aagaatatat cgccatgcgt ttaaccatta ttcctgaaaa aattgatcca aaagtggata
      121 tgcaagaacc acaagatctg atttattacg tgcacgaaga taaggttaaa gatcaagtga
      181 aagatgaaga ttatgaacaa cacgcgatca attacaaagt gaaaggatac aagaaagaac
      241 aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac cattgatttg aatgaagaat
      301 tagtgaagaa gctaaaagat caattgcctt atcaagtcgc agatgatcag gaaaatactg
      361 aaaacaagat taatagctac cagcaagaaa ttgaaacatc gaaaacgcaa ctgaaagatc
      421 aagaagagat cattcaaaaa cttcaagaaa aaattgatcg tatgacaaaa gaaaagattt
      481 agaaggacag gtggagtgaa aaaatgaagc gaacaaactt ggctgacaaa gatactttcg
      541 ctgagca
//
LOCUS       DACEND010000306          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.379, whole genome shotgun sequence.
ACCESSION   DACEND010000306 DACEND010000000
VERSION     DACEND010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.379"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW746_002772"
     CDS             complement(<1..132)
                     /locus_tag="IW746_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1079627.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACEND010000307          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEND010000307 DACEND010000000
VERSION     DACEND010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.denovo.380"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..>488)
                     /locus_tag="IW746_002773"
     CDS             complement(146..>488)
                     /locus_tag="IW746_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1079628.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTV"
BASE COUNT          129 a          104 c           76 g          179 t
ORIGIN      
        1 tttctgtagt aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact
       61 ctattctaca gaagggactt ccttttttct atttacacaa aatattttac actctcgaaa
      121 agtattgcat tttattatat aaaattcaga ctgtagacaa aagctgttcc aaactcgtaa
      181 ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa aaaaataaag
      241 gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca gcaaacacta
      301 gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc atcccatgct
      361 tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg taaattgatt
      421 tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc caaacatgtc
      481 tcgtgatg
//
LOCUS       DACEND010000308        38605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.009, whole genome shotgun sequence.
ACCESSION   DACEND010000308 DACEND010000000
VERSION     DACEND010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.009"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1444
                     /locus_tag="IW746_002774"
     CDS             <1..1444
                     /locus_tag="IW746_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1079629.1"
                     /translation="DNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSP
                     NLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELL
                     NIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFN
                     GKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEI
                     LIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPS
                     EHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPI
                     IDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPK
                     YILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISL
                     LEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            1441..3234
                     /locus_tag="IW746_002775"
     CDS             1441..3234
                     /locus_tag="IW746_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1079630.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            3434..3748
                     /locus_tag="IW746_002776"
     CDS             3434..3748
                     /locus_tag="IW746_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1079631.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            3769..4146
                     /locus_tag="IW746_002777"
     CDS             3769..4146
                     /locus_tag="IW746_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1079632.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            4156..4929
                     /locus_tag="IW746_002778"
     CDS             4156..4929
                     /locus_tag="IW746_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079633.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            4951..6354
                     /locus_tag="IW746_002779"
     CDS             4951..6354
                     /locus_tag="IW746_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1079634.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            6536..7720
                     /locus_tag="IW746_002780"
     CDS             6536..7720
                     /locus_tag="IW746_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1079635.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            7717..8007
                     /locus_tag="IW746_002781"
     CDS             7717..8007
                     /locus_tag="IW746_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079636.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8004..8225
                     /locus_tag="IW746_002782"
     CDS             8004..8225
                     /locus_tag="IW746_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079637.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            8267..9046
                     /locus_tag="IW746_002783"
     CDS             8267..9046
                     /locus_tag="IW746_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1079638.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            9113..9607
                     /locus_tag="IW746_002784"
     CDS             9113..9607
                     /locus_tag="IW746_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1079639.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            9663..10301
                     /locus_tag="IW746_002785"
     CDS             9663..10301
                     /locus_tag="IW746_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079640.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            10373..10768
                     /locus_tag="IW746_002786"
     CDS             10373..10768
                     /locus_tag="IW746_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1079641.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            10752..13205
                     /locus_tag="IW746_002787"
     CDS             10752..13205
                     /locus_tag="IW746_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1079642.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            13202..15229
                     /locus_tag="IW746_002788"
     CDS             13202..15229
                     /locus_tag="IW746_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079643.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            15226..16248
                     /locus_tag="IW746_002789"
     CDS             15226..16248
                     /locus_tag="IW746_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1079644.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            16265..17194
                     /locus_tag="IW746_002790"
     CDS             16265..17194
                     /locus_tag="IW746_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1079645.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            17439..17555
                     /locus_tag="IW746_002791"
     CDS             17439..17555
                     /locus_tag="IW746_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1079646.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            17571..19490
                     /gene="tet(M)"
                     /locus_tag="IW746_002792"
     CDS             17571..19490
                     /gene="tet(M)"
                     /locus_tag="IW746_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1079647.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            19588..19776
                     /locus_tag="IW746_002793"
     CDS             19588..19776
                     /locus_tag="IW746_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1079648.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19834..20187)
                     /locus_tag="IW746_002794"
     CDS             complement(19834..20187)
                     /locus_tag="IW746_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079649.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20718..21140
                     /locus_tag="IW746_002795"
     CDS             20718..21140
                     /locus_tag="IW746_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1079650.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21137..21367
                     /locus_tag="IW746_002796"
     CDS             21137..21367
                     /locus_tag="IW746_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079651.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            21865..22065
                     /locus_tag="IW746_002797"
     CDS             21865..22065
                     /locus_tag="IW746_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1079652.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22092..23285
                     /locus_tag="IW746_002798"
     CDS             22092..23285
                     /locus_tag="IW746_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1079653.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23353..24918)
                     /gene="guaA"
                     /locus_tag="IW746_002799"
     CDS             complement(23353..24918)
                     /gene="guaA"
                     /locus_tag="IW746_002799"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1079654.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24977..25900)
                     /locus_tag="IW746_002800"
     CDS             complement(24977..25900)
                     /locus_tag="IW746_002800"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1079655.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26096..26701
                     /locus_tag="IW746_002801"
     CDS             26096..26701
                     /locus_tag="IW746_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1079656.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27009..28025
                     /locus_tag="IW746_002802"
     CDS             27009..28025
                     /locus_tag="IW746_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1079657.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28166..29467
                     /locus_tag="IW746_002803"
     CDS             28166..29467
                     /locus_tag="IW746_002803"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1079658.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29513..30175
                     /gene="deoC"
                     /locus_tag="IW746_002804"
     CDS             29513..30175
                     /gene="deoC"
                     /locus_tag="IW746_002804"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1079659.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30196..30594
                     /locus_tag="IW746_002805"
     CDS             30196..30594
                     /locus_tag="IW746_002805"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1079660.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            30660..31742
                     /locus_tag="IW746_002806"
     CDS             30660..31742
                     /locus_tag="IW746_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1079661.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31954..33516
                     /locus_tag="IW746_002807"
     CDS             31954..33516
                     /locus_tag="IW746_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1079662.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33509..34642
                     /locus_tag="IW746_002808"
     CDS             33509..34642
                     /locus_tag="IW746_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1079663.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34642..35598
                     /locus_tag="IW746_002809"
     CDS             34642..35598
                     /locus_tag="IW746_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1079664.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            35697..36863
                     /gene="deoB"
                     /locus_tag="IW746_002810"
     CDS             35697..36863
                     /gene="deoB"
                     /locus_tag="IW746_002810"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1079665.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36887..37705
                     /locus_tag="IW746_002811"
     CDS             36887..37705
                     /locus_tag="IW746_002811"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1079666.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            37722..38429
                     /gene="deoD"
                     /locus_tag="IW746_002812"
     CDS             37722..38429
                     /gene="deoD"
                     /locus_tag="IW746_002812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1079667.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        12851 a         6585 c         7901 g        11268 t
ORIGIN      
        1 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
       61 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
      121 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
      181 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
      241 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
      301 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
      361 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
      421 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
      481 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
      541 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
      601 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
      661 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
      721 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
      781 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
      841 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
      901 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
      961 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1021 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1081 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     1141 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     1201 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     1261 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     1321 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     1381 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     1441 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     1501 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     1561 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     1621 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     1681 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     1741 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     1801 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     1861 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     1921 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     1981 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2041 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     2101 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     2161 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     2221 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     2281 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     2341 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     2401 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     2461 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     2521 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     2581 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     2641 agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt
     2701 aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat
     2761 agtggagacg acaacggtgt tgatttatac aaatttgtta ccaaacatat atttgttaat
     2821 cttaatattg acgagaaaaa ttacccgtct atttttaagc actatctatt ttttattaac
     2881 gaagtagaag atcggataaa aaataattca ttatctagta gtgtatctag ttttaagaag
     2941 atgtttaaat ctcgagatgg tatcacactt actacagcac ataaagttaa aggaaatgag
     3001 tatgatactg ttatcgctat taatcttcta aacgggaaaa ttcctcattg gaatgaaatt
     3061 tttaatgttt ctgataaagg aattagttca gcgagaaaac tactatacgt aatttgttca
     3121 agagcaaaaa ataatttatt tcttttctca gaggtgggtt attttacgag taaaggagac
     3181 ttgctttatc ccactcaatt attagataaa atagaatttg attataatat ttaaaatacc
     3241 taggtagtcg ttgttttaaa tatagtagga atcggacgct cagtacggag cgtctatttt
     3301 tttacccaat tttgaaagga agtgaactta tgaaaataaa aaatcaagaa tcaaaaggtc
     3361 gctctccccc ctttaaaacc atcacacaaa taagattcat tcaaacataa aaacagaaag
     3421 gataggtaaa aatatggaac ttaaatatgt cattcccaat atggaaaaaa catttggaca
     3481 attagaatat gctggtgaag ggaacattga acaacgtcga ataaatggca gaccaaccat
     3541 tctctctcgt agttacaatt tatactcaac gattcaacga gcagatgata ttgttgtcat
     3601 tcttccctcc gaagctggcg agaaacattt tgaatccgac gaaaaagtca aactcatcaa
     3661 cccacatatt accgcagaag gttataaaat tggcaatcga ggatttacca attacattat
     3721 gcacgcagac gatatggtga aagcataaga aaggaaaggt gaaaaaatat gagattagca
     3781 gaaggtattg tgattgaaaa agaccagaca tttggcacat tgaaattttc cgccttgcgt
     3841 cgtgaggttc gtatccagaa tgaagatggt acggtttccg aagaaattaa agaacgtacc
     3901 tatgacttaa aatcaaaagg acaaggacgc atgatacaag tcagtatccc cgcttctgtt
     3961 cctttgaaag aatttgacta caacgcagaa gtggaattaa tcaatcctgt ggcagatacc
     4021 gtagcgacgg ctacttttcg aggggcagac gttgattggt acgtgaaagc agacgatctt
     4081 gtattaaaag gacagacaaa acctcatcag aaatataaaa aagaagcgtc cgaaagtgaa
     4141 aaataggtgt tgcttatgac aaacacacca ttgattttga aagaaattcc gaaggatgaa
     4201 gccatttctt ttattcggca ataccattat tcaaagatac tccctagatt gtgtaaatac
     4261 tttctaggga ttttttcaga agaaaaatta ttaggcgttg tggaattagg ctggggtacg
     4321 caacccctac aaaccatacg caaactcttt cccgacagtt cccttcaaac cacagattat
     4381 ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta ttttggcagt
     4441 caagctcttt cagctctaat taagtggttg aaagaacata cggactgtca ttttttatat
     4501 acactggcag atggaattga gggaaaatgt ggctatgtct atcaagcgtc caacttcttt
     4561 tactgcgggt atttcaagac aagtgtctat cgtgataagc agtcatggga aaagatacac
     4621 cctcgaagtg ctcgcctttt attagaggaa aatgctcgct ttgaacaggt agagaaaaag
     4681 cattggcttt cccaggcatt ttgcgaatac aaagggattg agaaaatcaa tggacgcatg
     4741 tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca cactctctat
     4801 cgacgtcatt attatccgaa agaaaaaaat ttacgctttg aaaagcgaat cgcttatcag
     4861 aaatatgaag ccatttctca accaactttt gataaacagg ctcgtatcta caatacacaa
     4921 ttattttgat aggtggaaag gaggattttt atgccattgc ttcatgacag aggagagcga
     4981 atcaaagcca acgataagca cttggtctat cattttgtac taatttcatt gctggtagtt
     5041 tttctagtcg tcctactccc ttttcattgg agagcgttac aatcggtgaa ctggcaagca
     5101 gttcagttag atacattttt ctatcaactt catacgccat acttttggag cagtattctc
     5161 actgctcttt ttgtatgcgg acttatcagc tggctttatt atcactatcg tatagattct
     5221 ataaaacagt tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa tcattggtat
     5281 gaagccgaag aaactcaatc agagggattc tttaaagatt tacccagcaa gaaaaccaaa
     5341 caacgaatcc gccattttcc taaaatatat tatcgaatga aagatggact aatccatatt
     5401 catgtagaaa tcaccttagg gaaatatcaa gatcagcttt tgcacctaga aaacaaacta
     5461 gaaacaggat tgtattgtga attagtggaa aaaacgttga aagattctta tgtagagtat
     5521 gtcctacttt atgatactat tggcaatcga attggcattg aagacgtggt agctcaagat
     5581 ggacgtgtca aattgatgga atcggtctat tgggagttcg attctctccc tcacatgtta
     5641 attgctgggg gcactggtag tggaaaatcc tattttattc tcacattgat tgaatctctc
     5701 ctgcatacaa atgcaaagct ttatattctt gaccctaaaa atgctgattt ggcagactta
     5761 ggggcggtca tgaataatgt ctattaccgt aaagaagata tgttggcatg tatcaatcaa
     5821 ttttacgaag atatgattga tcgcagtgaa accatgaaac aacaccctaa ctataaaaca
     5881 ggagaaaatt atgcttatct tggtttgtct gccaattttc ttatttttga tgaatatgtg
     5941 gcaatgatgg atatgctagg tcgtgaaagt accaccgtta ttcataaact caaacaaatt
     6001 gtaatgttag gacgacaagc aggcttcttt ttaattcttg cctgtcaacg tccagacgca
     6061 aaatatttta gtgatgggat tcgggacaac tttaacttcc gtgtggcttt gggtcgtatg
     6121 tctgaaatgg gctttggtat gatgttcgga agcgatacac aaaaacaatt tttcttaaaa
     6181 cccatcaagg gaagaggtta cgtggataca ggaaaaagtg tgattagtga gttttataca
     6241 ccccttgtac caaaaaggta cgatttctta ggagaaatag gaaaagtcat tcaaaaaaag
     6301 caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct ttgattggtg
     6361 tggcgttagc cacgccaatc ctttagcccc ctctatctaa caggggggca caaatacaac
     6421 tgaaaacagg caattctcat caaccaatgt tgatggtaca aaggattgcg gaaaatatga
     6481 aaggtgtaaa ctcaagggtc atagtttaca tcttttttag attggaggtt tgcgaatgga
     6541 aaacgaaaaa atttggatta aggaattgaa agaaaagcga ctggcttatg gcgtttctca
     6601 aaataaactg gcggttgctt ctcatattac cagaccttac ctatcggata ttgaaacagg
     6661 caaggcagtc cctactcaac aggtgaaaga agatttactg aacgctttgg aacgcttcaa
     6721 tcccgataac ccattggaga tgttattcga ctatgtacgg attcgattcc ctaccaatga
     6781 tgtggctcaa attatcggtg aggttttacg tttgaatatg gattatatgc tacacgaaga
     6841 ctttggctat tattcttatc ccgaacacta tcgtttcggt gatattgttg tcctagtctc
     6901 tcatgatgta agtaaaggtg ttttattaga actgaaagga aaaggctgtc ggcaatttga
     6961 aaactttcta ttggctcaac atcggagctg gtatgatttc tttcaagatt gcatggaaca
     7021 taaaggtatt ttcaaacgct tggatttagc tatcaacgat aaaacaggga ttttgaatat
     7081 tccagatttg gcaaagaaat gtaaacagga agaatgcatt tccgtctttc gaagctttaa
     7141 aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg gaaacacact
     7201 ttatattggc tcattaaaaa gtgaagtcta tttctgtata tatgagaaag attatgagca
     7261 atacgtgaaa aatgatgttc ccttagaaga cgcagaagta aagaatcggt ttgaaattcg
     7321 gttaaaaaat gaccgagcca cccaagccat gaaagattta ttggcacatc aacaggcgga
     7381 aaaaacagcc tttgaaatta tcaatcgcta tatccgcttt gcagataaag acgacactaa
     7441 acgtcggagt gattggaaaa ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg
     7501 agcattacgc ctaaccacac agccagagcc ttattctttt gaacgaacat tgaactggtt
     7561 acatcatcaa gtcgccccta cattaaaaat cgcaagcatt ttagatgttc tcaacggtac
     7621 aaccattatc tccaccatga ttcaggaagc caaactgaca gaaaaacatg agaaattaat
     7681 tgaacaacaa catctagcca tggaggattt gattacatga gccttttaac aaaagaatta
     7741 aagaagttag ggtttcagtg tggcattgag tttcaagcgt atatacaaaa tacagggaaa
     7801 tatacgtctc tcattattga gggaaaacgg caagctggtg ataccatcta tacctatgat
     7861 ttttacaagg tgagatttta taataattat accaatcgag taaccgtcta tggagagcat
     7921 ttaacccctt ttcaattatt aaggcgtgta aaaagctata tctattatcg agaaaaatat
     7981 cttaaggaaa ggaggacaat cacatgaatt ttgggcagaa tttgtacaac tggttcttgg
     8041 ataatgcaca gtcattggta ttgttagcga ttgttgtcat tggactgttt ttaggtttta
     8101 aacgagaatt ttcaaaactc attggcttct tgattattgc tctaatcgcc gtcggacttg
     8161 tgttcaatgc agctggcgtg aaagatgtgt tattaaactt atttaaccga atcattggtg
     8221 cttaacagat atttttcatg ataaaatagg aagtatatat tttgttatga aaggatttat
     8281 gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag
     8341 ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt
     8401 aactttagat gagactgacg attttactta ttataaagga tataaaaagg gctggaatac
     8461 gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca
     8521 taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt
     8581 agtggatact attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt
     8641 gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg
     8701 tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat
     8761 actaaaaaaa tatgaaatta cagttcctag agacgaaaat ctatctaaat tatatagtag
     8821 gatacaacaa ttaattgaaa aagaaattca acctactgaa cttgctgata tagtgaagac
     8881 aacgataagg tcatcaaatg gaatgattag ctctcttaat gaagcaagaa ataggcattc
     8941 acttgcacac cctaatacaa atattattgg aaagagagaa gcaaaactaa taataggtat
     9001 ttcaagtaca gttacagatt atattagcgg ataccttgat aagtagcgtc attaactgat
     9061 tgctactttt tttatgacta tttttataaa aaggatgtga taagaattgg aaatgaaagt
     9121 ctatgtggca aatttaggac ggtataatga aggtgaatta gtcggagcat ggtttactcc
     9181 accaattgat gaggaagaaa tggcagaaag aatcggttta aatgaggact acgaagaata
     9241 tgcaatacat gattttgagc ttccttttga tgtggacgaa tacacgccga ttagtgaaat
     9301 caaccgtcta tgtgaagcca ttcaagaaat cgaaggtaca cctatttata atgaactcaa
     9361 agaaattcaa ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca aagaggatat
     9421 tcattgttat agtgattgtg attcgatgga agatgtcgcc cgttattacg tggaagaaac
     9481 aggacagctt ggtgaagtcc cttccaattt acaaaattat attgactacc aatctttggg
     9541 acgtgatatg gaaatagagg gtaattacct agttacctct catggggtat ttgaatattg
     9601 tcagtaagtt taacaatccg tgttataatt aaattaataa atagcacgga gaggtataaa
     9661 ttatggtaaa tgtatttgaa aatcttaaac tattaaaact agacatgaaa gataaaggct
     9721 gggtgataga ttccttttac tttcgttaca aacagcaaaa ttatattgta cttgttaagc
     9781 tatttgaaaa agaggaaaaa gttccagaat atgctctgtt aaaactagaa tttttaaagg
     9841 aaaacgattt ttcagatatg ctagctgtct atgctaattc ggtaaaatta tatacagaca
     9901 cgaaaacaat aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat
     9961 ttcaatttag ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg
    10021 aagaccaaaa agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa
    10081 aaaaatattg tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac
    10141 gaagtccttt taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag
    10201 ggtcagatac caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg
    10261 aaggtattat tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca
    10321 atcacctatt ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa
    10381 aataagaagt tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt
    10441 acaactccct ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt
    10501 cgtcatactc tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata
    10561 cttagggatt cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa
    10621 accctatgga tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc
    10681 tggcaaacaa atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag
    10741 tgaacaacta gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg
    10801 atggcgaagt attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg
    10861 atgaaaaaat tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca
    10921 agattcatgc tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga
    10981 aaaaagaaat cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg
    11041 aagcgttaat ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta
    11101 aactctacct caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag
    11161 catttgcgga attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc
    11221 ctatggcgga aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca
    11281 gacgctttca gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt
    11341 acgggcaaac aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag
    11401 ataaaaaagt gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag
    11461 aaaatcaacg ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta
    11521 caattaatag tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc
    11581 aacagcagtt tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa
    11641 aagccttatc taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat
    11701 gggaaagcga caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat
    11761 tagaaagtaa tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg
    11821 tatcagcaaa gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg
    11881 atgatctgtc tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat
    11941 ttattccctc aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt
    12001 tggctggtct tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg
    12061 gttacaatct cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa
    12121 ttaaaggctc tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa
    12181 aatcattctc taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca
    12241 ttgttgaccc aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg
    12301 aaatcaatat tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg
    12361 tgattttaaa actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa
    12421 caggtatttc cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg
    12481 taactcaaag caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata
    12541 cgcccatcag caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc
    12601 atctgttatt cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata
    12661 ttattcaagt ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta
    12721 ccatggaatt attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta
    12781 tccactctga ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac
    12841 aagtggcaca aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg
    12901 caggggtcta tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata
    12961 atattggctt gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag
    13021 aattttttgg tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa
    13081 atggacaatg tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga
    13141 tttttgaaga gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag
    13201 aatgacacaa aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg
    13261 cgttatttta cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga
    13321 tgaaaccatt gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga
    13381 ttattatgtg gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca
    13441 atcggtgatg tatggattgt atgccattac taattttatt tggacaattt ctctttatct
    13501 atcgaacgcc acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac
    13561 atcccaagca attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc
    13621 cagttcaggg ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt
    13681 tgcctataca ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt
    13741 tgtcgtggtg tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa
    13801 aatcaatgat ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt
    13861 tcttcctaac acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt
    13921 ctctgttcaa gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc
    13981 gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga
    14041 agatagagaa aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct
    14101 accgaagacg atttccagat taggaacagt cttctttttg tttgtattca atattggtat
    14161 ctcgattttt gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat
    14221 tttcgctatg tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat
    14281 gggcaggcat gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt
    14341 gattattacc gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc
    14401 ctttttcatg attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg
    14461 cgatttaatg agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt
    14521 gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag
    14581 taagattttt cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa
    14641 tcaaaccaat aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac
    14701 agcactttcc aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac
    14761 agcccatgta ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca
    14821 gatacaggcg acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac
    14881 tcataatgta acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca
    14941 gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga
    15001 taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca
    15061 gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga
    15121 aaatcatcag cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa
    15181 tcagaggaag ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata
    15241 acagtgattt gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt
    15301 ttctttgcag atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc
    15361 ggtgtgaccg tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag
    15421 gaatatggga ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc
    15481 ggtacagcag aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg
    15541 agtaccgaag agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca
    15601 gaacaacaag gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt
    15661 ttaaattatg tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct
    15721 aaagagaagt cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat
    15781 ggtggttggc gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta
    15841 accgacactt ccccaacaga atttgatggt gaaaccgtgc aagtcattat ggacgaagcc
    15901 ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat
    15961 tgtagtggct taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc
    16021 caagatcagt atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt
    16081 atcttctttc attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta
    16141 gagggaaatc gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt
    16201 tattggcaag accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa
    16261 tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa
    16321 accgagcaag caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt
    16381 tgttttatgg gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc
    16441 cattgacact catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa
    16501 ccaagtagaa agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca
    16561 agaagccatt gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt
    16621 attaaatgaa gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca
    16681 ggtgtggcag gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca
    16741 agttattacc gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca
    16801 tatagatgaa tcagataata tggttattat caaaaatccg acaatgagta agaaacctca
    16861 aaaatcggac taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat
    16921 ggacgaaatc actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa
    16981 agaattgact tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt
    17041 cgaggaattg gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc
    17101 tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt
    17161 agaaaaacaa gataattgga agattgtaaa ataacaaata ttggtacatg attacagata
    17221 ctttgtaatc atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt
    17281 acgtgctatt atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct
    17341 taaataaact gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc
    17401 ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga
    17461 atatctgtat gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt
    17521 atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta
    17581 ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca gaaagcttat
    17641 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
    17701 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
    17761 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
    17821 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
    17881 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
    17941 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
    18001 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
    18061 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
    18121 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
    18181 aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta
    18241 atatagggat tgataacctt atagaagtta ttactaataa attttattca tcaacacatc
    18301 gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac
    18361 gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
    18421 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
    18481 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
    18541 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
    18601 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
    18661 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
    18721 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
    18781 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
    18841 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
    18901 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
    18961 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
    19021 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
    19081 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
    19141 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
    19201 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
    19261 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
    19321 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
    19381 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
    19441 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
    19501 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
    19561 aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt
    19621 tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta
    19681 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
    19741 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
    19801 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
    19861 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
    19921 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
    19981 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
    20041 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
    20101 ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa
    20161 atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta
    20221 tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac
    20281 tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta
    20341 cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta
    20401 atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc
    20461 ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag
    20521 attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt
    20581 tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca aatccccctt
    20641 tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca
    20701 gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg
    20761 actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca
    20821 gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc aactcttttt
    20881 ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga ttaagaatca
    20941 atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca
    21001 ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag ttactaaatt
    21061 tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt aaagaattca
    21121 tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc tcattgtaaa
    21181 ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc gagggtatac
    21241 ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata tggttattga
    21301 tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat cgttcgaaat
    21361 taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg
    21421 aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag gctaacaagc
    21481 caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag aaaagagctg
    21541 atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt aattcgaaag
    21601 taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac tcccgtatag
    21661 ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga gaaccatgca
    21721 gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat tcttctcttg
    21781 tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga gttttcctag
    21841 cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt agttgagcta
    21901 ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt gatgattaca
    21961 aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga agctgttgaa
    22021 gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt gcacagtaag
    22081 gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc tattattttc
    22141 gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt agtggattca
    22201 ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa ccagaagaat
    22261 taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt ttccttccat
    22321 ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact attaagaagc
    22381 atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat gtgcaaaatt
    22441 ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt caaggactat
    22501 tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat ccatctaaga
    22561 ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa gaagaatttg
    22621 aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta tttatctctc
    22681 tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt caatgggaag
    22741 atattgattt tgactcagga gtactaacca tcaacaaaac actctattac aagaatcaat
    22801 cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata
    22861 aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta attcagacag
    22921 actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca tatgctattg
    22981 aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta cggcattctc
    23041 atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat cgtctaggac
    23101 acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac agtaatttcg
    23161 aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa aattatgata
    23221 cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg aaaaaagtgc
    23281 aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag aggatttcta
    23341 gctctcctac aactattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
    23401 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
    23461 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
    23521 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
    23581 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
    23641 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
    23701 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
    23761 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
    23821 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
    23881 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
    23941 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
    24001 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
    24061 tgcatcgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
    24121 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
    24181 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
    24241 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
    24301 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
    24361 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
    24421 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
    24481 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
    24541 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
    24601 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
    24661 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
    24721 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
    24781 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
    24841 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
    24901 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
    24961 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
    25021 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
    25081 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
    25141 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    25201 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    25261 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    25321 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    25381 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    25441 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    25501 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    25561 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    25621 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    25681 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    25741 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    25801 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    25861 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    25921 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    25981 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    26041 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
    26101 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    26161 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    26221 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    26281 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    26341 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    26401 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    26461 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    26521 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    26581 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    26641 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    26701 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    26761 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    26821 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    26881 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    26941 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    27001 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    27061 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    27121 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    27181 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    27241 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    27301 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    27361 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    27421 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    27481 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    27541 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    27601 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    27661 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    27721 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    27781 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    27841 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    27901 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    27961 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    28021 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    28081 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    28141 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    28201 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    28261 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    28321 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    28381 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    28441 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    28501 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    28561 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    28621 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    28681 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    28741 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    28801 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    28861 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    28921 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    28981 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    29041 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    29101 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    29161 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    29221 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    29281 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    29341 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    29401 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    29461 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    29521 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    29581 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    29641 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    29701 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    29761 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    29821 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    29881 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    29941 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    30001 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    30061 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    30121 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    30181 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    30241 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    30301 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    30361 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    30421 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    30481 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    30541 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    30601 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    30661 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    30721 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    30781 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    30841 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    30901 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    30961 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    31021 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    31081 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    31141 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    31201 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    31261 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    31321 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    31381 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    31441 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    31501 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    31561 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    31621 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    31681 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    31741 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    31801 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    31861 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    31921 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    31981 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    32041 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    32101 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    32161 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    32221 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    32281 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    32341 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    32401 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    32461 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    32521 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    32581 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    32641 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    32701 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    32761 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    32821 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    32881 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    32941 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    33001 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    33061 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    33121 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    33181 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    33241 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    33301 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    33361 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    33421 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    33481 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    33541 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    33601 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    33661 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    33721 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    33781 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    33841 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    33901 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    33961 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    34021 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    34081 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    34141 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    34201 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    34261 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    34321 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    34381 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    34441 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    34501 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    34561 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    34621 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    34681 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    34741 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    34801 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    34861 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    34921 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    34981 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    35041 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    35101 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    35161 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    35221 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    35281 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    35341 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    35401 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    35461 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    35521 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    35581 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    35641 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    35701 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    35761 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    35821 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    35881 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    35941 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    36001 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    36061 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    36121 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    36181 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    36241 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    36301 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    36361 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    36421 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    36481 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    36541 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    36601 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    36661 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    36721 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    36781 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    36841 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    36901 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    36961 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    37021 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    37081 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    37141 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    37201 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    37261 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    37321 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    37381 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    37441 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    37501 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    37561 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    37621 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    37681 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    37741 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    37801 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    37861 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    37921 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    37981 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    38041 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    38101 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    38161 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    38221 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    38281 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    38341 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    38401 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    38461 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    38521 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    38581 gagtcacaag gaacaatttc aaaaa
//
LOCUS       DACEND010000309        14399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.067, whole genome shotgun sequence.
ACCESSION   DACEND010000309 DACEND010000000
VERSION     DACEND010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.067"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            86..697
                     /locus_tag="IW746_002813"
     CDS             86..697
                     /locus_tag="IW746_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1079668.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            1727..2899
                     /locus_tag="IW746_002814"
     CDS             1727..2899
                     /locus_tag="IW746_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1079669.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            3277..3840
                     /locus_tag="IW746_002815"
     CDS             3277..3840
                     /locus_tag="IW746_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079670.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            3827..4711
                     /locus_tag="IW746_002816"
     CDS             3827..4711
                     /locus_tag="IW746_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ1079671.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            5105..5602
                     /gene="dfrG"
                     /locus_tag="IW746_002817"
     CDS             5105..5602
                     /gene="dfrG"
                     /locus_tag="IW746_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1079672.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(5674..7626)
                     /locus_tag="IW746_002818"
     CDS             complement(5674..7626)
                     /locus_tag="IW746_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1079673.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(7633..7929)
                     /locus_tag="IW746_002819"
     CDS             complement(7633..7929)
                     /locus_tag="IW746_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079674.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8495..8749
                     /locus_tag="IW746_002820"
     CDS             8495..8749
                     /locus_tag="IW746_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1079675.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            9146..10747
                     /locus_tag="IW746_002821"
     CDS             9146..10747
                     /locus_tag="IW746_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1079676.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            10981..11280
                     /locus_tag="IW746_002822"
     CDS             10981..11280
                     /locus_tag="IW746_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1079677.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            11414..11791
                     /locus_tag="IW746_002823"
     CDS             11414..11791
                     /locus_tag="IW746_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ1079678.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            11840..14203
                     /locus_tag="IW746_002824"
     CDS             11840..14203
                     /locus_tag="IW746_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1079679.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
BASE COUNT         5300 a         1948 c         2593 g         4558 t
ORIGIN      
        1 atttgatggt atagagagtc tgctgaactc acgaaaaaat tcctagttaa ttaaactaga
       61 gtaaaattat ttgtaggtgt taaatatgtt agttggctat gcacgagtca gttcagatga
      121 tcaaaatttg gaaagacagc ttgaagaatt tagaaaaatt ggtgtcgaaa aagtatttgc
      181 tgagaaaaaa tctggacgta acacagtaga acggccagag ttcaataaaa tgttgtcatt
      241 tgtcagagaa ggggatacgc ttgtttttga gtcgcttgaa cgcttaggaa gaaattctga
      301 tgaaatacta gaaacagtat cttttataga taaaaatggt atgggcttaa tggttttgaa
      361 tttaccaatt ttagatacaa aatttggtga tccaaactta gaaaaacttg ttagaagctt
      421 agtaattaac attttttctt ggacggctca aaatgagagg gaggaaatta agagaaaaca
      481 aaaacaagga atagcaattg cgaagaagaa aggagtctat aaaggaaaac cctacgaata
      541 ttcttctact gcaaagaatc cacaaaagag gtttatctat aatgaaatag taagaatgct
      601 tggagaaggt gttccgatta agcgaattgc agaaaaaaca ggaacaaata gaaatttagt
      661 ctatagaata aagaaaagaa taagtgaatc tgattgattt taagttcttt gagaaatcaa
      721 tgttacgaga gaaggattct aagcctgttt caaactttta aggtgtacaa ttatacaaag
      781 aagcttctaa acaaaagaat taattttgtg gcagtcaagt aacgacagaa atagaaaaga
      841 aagaggctat gcgagagtga atcaacaagt taattgggtt aatgattttg aaaaagaatt
      901 tcgactgaaa ttttttggaa atcctacagc agaaagtaca gaagaaaaaa attatgactg
      961 gtcagaattt taggttgcat attcgattca ataagtgttg aagataatag ttctcttacg
     1021 ccacagtata caacgttaga atatgacgaa aacagattca atacatttcc ttgcaatacc
     1081 attcaagaat caattgataa gcatgagtga taattgatta tctacaggaa ggtgtttttt
     1141 atgaaactag agatcaaaga agttgtctgc gattggggaa tttatgtaga cggagaaaca
     1201 tatccattta tgatttttaa tagtaaagca aatgcacaag agataatgcg tatcatggag
     1261 ttggataata agcacgaaag atttgattaa gtaaaaaaat ggagaatgaa tgtgaagcag
     1321 tggaatacca aaaaacatgc gccaataata agtatatttt aggtgcaatg ggattaggta
     1381 aatcagcttc tattaaacag gtctacgaag aatcacagga gaattgcgta tatgaaaatt
     1441 cgaattaaag agtgagaata tgtgggagct aatttattta gttttatact taataggaaa
     1501 atcaactgaa tttgtatata acaataaggt aatatttata ttgttcttaa tagtttgtat
     1561 tatcgtattt gatgtctatg atttactaat tcataagaat gaaaatagta atattggtag
     1621 aattagataa agtccgtata attgtgtaaa agtaaaaagg ccatataaca gtccttttac
     1681 ggtacaatgt ttttaacgac aaaaacatac ccaggaggac ttttacatga cccaagtaca
     1741 ttttacactg aaaagcgaag agattcaaag cattattgaa tattctgtaa aggatgacgt
     1801 ttctaaaaat attttaacaa cggtatttaa tcaactaatg gaaaatcaac gaacagaata
     1861 tattcaagca aaagaatatg aacgaacaga aaaccgacaa agtcaacgaa atggctatta
     1921 tgagcgcagc tttacgacac gtgtaggcac gctagaatta aaagtaccca gaacacgtga
     1981 tggccatttt tcacccacag tgtttgaacg ttatcaacga aacgaaaaag ccctcatggc
     2041 ttcaatgttg gaaatgtatg tatcaggcgt ttcaactcgt aaagtatcaa aaattgtgga
     2101 agaactttgt ggtaaatccg tctctaagtc cttcgtttct agcttaacag aacagctaga
     2161 acctatggtt aacgagtggc agaatcgttt attatcagaa aaaaattatc cttacttaat
     2221 gaccgatgta ctctatataa aagtacgaga agaaaatcga gtactctcaa aaagctgtca
     2281 tatagcgatt ggaataacca aagatggcga ccgtgaaatt atcggcttca tgattcaaag
     2341 tggcgaaagc gaagagacct ggacaacatt ttttgaatac ctaaaagaac gcggtttaca
     2401 aggtacggaa ctcgttattt ctgatgcgca caaaggatta gtctctgcca ttagaaaatc
     2461 cttcaccaac gtaagttggc aaagatgcca agttcacttc ctaagaaata tctttaccac
     2521 cattcctaaa aaaaattcaa aatctttcag agaagctgtt aaaggaattt ttaagttcac
     2581 agatattaac ttagcgcgtg aggctaaaaa tcgattgatt catgattata tcgatcaacc
     2641 aaaatattca aaagcttgcg catcattgga tgatggattc gaagacgcct ttcaatatac
     2701 cgtacaagga aattcccaca atcgactaaa gagtaccaat ctaattgaac gactgaatca
     2761 agaagtacgc agaagagaaa agattattcg catcttcccc aatcaaacat cagccaatcg
     2821 cttaattgga gccgttctta tggacctaca tgatgaatgg atttattctt caagaaaata
     2881 catcaatttt gataagtaga aatggtaaaa acattgtata gcattttaca caggagtctg
     2941 gacttgactt agaattataa tcacaaactt aatagtaatt actattggga ttacaacatt
     3001 aacaaataaa gctttaatat caagtatgat tttaatagtg tttgatttta ttatcattta
     3061 tagtttgctg tttttaataa agaaagtaac tgaatattta aatccgttag gcacgaaata
     3121 aattttaatt gaggttgaat tatagcggta aatcatacaa aatagaaatg tagtcttgtt
     3181 aagcgtcttg actatctaat tttgaatgag gagggttgta tatagaaaaa gggtagttaa
     3241 attcgtagat caaaaggcta cgaaggagaa taaagaatga cagctattta taaagcacta
     3301 aaaaattcta acgtatcaga gtacgttaag tcgagagtat taaataataa tttagattac
     3361 attttagaaa atggtgttgc tgatttaatc agtaaattaa gtgatcctat agatatagat
     3421 catttggtgg tatggcaaga tggtacttta gaagtatcaa tatatggaga attttatagt
     3481 agaaataaag taactacatt caaaaatagt attagtggtc gagaagattt aaaaggaatc
     3541 aataaaaata gtaaattcta tagaacaaat aaagaattgc tagaaaaaat ttcaaagcgt
     3601 gtttttgaat tggtgaatcc attttcaaaa aaacgtagta aagaactttt tggaattttt
     3661 tatgatggtt ttagtgcaaa aagtaatgta gaaaaatttt tttattttaa tgattcagct
     3721 tatattgcgg ttaaagacag taaaagaatc cctgaagcaa ctttagaata tttagagcca
     3781 attaaattgt ctgtttttta ttcactttat gagaaagaaa tcgctgatga agcggtttaa
     3841 ggggagtaaa atgagtaaaa aaataggcaa agagtcatta tcgctcggat tgaaaaaaga
     3901 aattgttaaa tttaaaagta tgtatagaga gccggaattt agactcattt ttcaattaga
     3961 tatagtgaga gggctgaaaa ttagttttag ttgtttaggt tatctggtct ggctattcat
     4021 tgctagagta ggatggagca atgcttatag cttttataaa ctgttcgccg gaactgatcg
     4081 attaaaagaa tcattagagg tgaaagaagc cttaaatatt ttaggaagtc ttgctttcag
     4141 cctgtttatt tttgctttat ttatcttaca agtaggaaaa ataatgatgt caacaaatga
     4201 caattatatt aattatttga agccttttca aaaaggaata aaaatcattt gtagacaggg
     4261 cacagtttta tttctactta gcctgtatga gcatgaaatt cattttcaaa attattataa
     4321 tacgacaact aaacttggtg catttttact cctatattta gttagtaata tagctgaaaa
     4381 aatgctgttt aaacatattg taaagcaagt cttttctgat attcaaaagg aaagctattg
     4441 tttaaaaaca ccttatggaa atttaaaaaa tgttgagctg tttttattta aaatggatag
     4501 taagtgggat aaatatatct atagaagtaa agaagtattt ttaactggta gttataacaa
     4561 aactcttcag gagagcctgt acgacacagg agcaatattt aataatgaac ctatggtttt
     4621 gaaagatttc tttggagatt atgaactagt ttggaatcca aaacagctta gtgaacaggg
     4681 ggcagaaaat gaatttgctg aagttcacta acatagtgta cagaaaaaca ttatctgaaa
     4741 gaagaacgcc ttcaaaagaa agtacaagaa cgattgggcc gtacagaaga ctaacgaaag
     4801 atcgtaaaaa aaaggaaaga caatgaaaat taagtagttc agttttggag tacaaaatga
     4861 caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca aattacatac
     4921 aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg
     4981 tattatgata atataactta tagaaaaaat tgcttattca actaacgggc aggataatga
     5041 gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt
     5101 ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa
     5161 tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca
     5221 tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag
     5281 aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc
     5341 aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga
     5401 acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca
     5461 tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt
     5521 tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga
     5581 aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc
     5641 acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca
     5701 atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc
     5761 tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc
     5821 atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa
     5881 tggtctttca agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg
     5941 gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt
     6001 tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt
     6061 atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg
     6121 acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa
     6181 tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca
     6241 ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct
     6301 taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat
     6361 cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt
     6421 ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa
     6481 ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata
     6541 agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaattt
     6601 tcttgtatct tcatttaatt catccattga aatattccaa tcataggcaa catcagaacg
     6661 gaacacatat cttgataaga cctcagcaga tacaactata tacgtttgca tattaaggtc
     6721 ttcaaacccc gtatatttct ttgaaccttg tcctttctta cggacgttat ttaggtagta
     6781 gtgcatcttg tccgtattaa gccacatctc gttaaatttc tttgtttgaa ggggttgtgt
     6841 ctcatatcgt tctaaaaact ccaaacaacg cctatacagc cactccagct tatgatagta
     6901 ggttccaact ccaattccta aaatatcaca agtacgactg actgaaaccc ttccaacaac
     6961 catcttagcg aacattggaa gaatggtgtt cttttgttgg tgataagtag tggtttcttc
     7021 tctttttggt agaacattag tgaacttctt acacgcctta cattgatagc gttgagattt
     7081 gcccctactt ttcccccttt tgtaaaattg cttcggctca ttgaatgggg ttgattcttc
     7141 ttccgagcag ccttctttat gaaattgata atctggctca acatcttgga tgctattgat
     7201 ttctataagg cgtttaattt cttcaacaac agaccaatta gataaagggg ttactgtaca
     7261 gttttgcgat acacctctgc caataggatt gtcattgcag tacatacctt tatgaccttt
     7321 atctttggaa ctaccagtca ttttatatcg acttggtttc ccttttacat ctttgaattt
     7381 gtgctgttct ttcccaaaat tacaacaaaa cggattgatg cagtaattat attgaatggg
     7441 atattcagaa ccgttccatt taaaagacgt tggcaagaac aacattttgt tataccttgt
     7501 tgcaaatcgt tttggagtta ataaatcata ttgtttagcc ctatctttta attcttcgtt
     7561 tgaaatcgga attttaacat ctacaatttc atcttccttc gtggctaatc ttttaagctt
     7621 ggtcattgtc tatcaaccca ctctatcttt gatacccatt ttttcaacaa tccgcttatt
     7681 acttcgcttg ttggcaatcc attcaataaa cttcttatcg tctaaaatgt tcaataagaa
     7741 ttcatccact gcttcgcttt cagtacgctc tgcatattca gcgtattgct taactatcac
     7801 ccttacttgc tctgatattt cccaatccac agacttagcg ttcttatttt ttggctcaag
     7861 aaattgcata ggaaaatgca cccctttttg cttagtaaat tgtaaccatt ttaacacacc
     7921 aatttgcatt cctacaataa ttatttcacg ttcgcatttt tatatacatt ttaacttaca
     7981 ttttgtggtg taaattaaca acaaacgttg atttaacaat aaaaaacgag tatcaaattc
     8041 agttgcaatt tgatactcat ttaggtttac attttcactt tttgtactcc aaaactgaac
     8101 tacttaaatg aaaatgtctt tccttttttt ttgactacaa gtagcctgaa aatgaaattt
     8161 caaaagacta caagtagtct tttgtggcaa aaataaagag ccaacgaaag ctaccaacaa
     8221 tcgttgactc gtaagactgt tcgtagtaag aaaaatcttt ggcaatccac gaagtcttta
     8281 tttcataatt tatataaatg attatacagt cactttcctg aatttgcaag taaaaaagct
     8341 aagaaatatt gtttaagcgt ttccttaaaa gaatctttaa ataaattttt ttccaattga
     8401 aaacatgact acaagtagac tatttctttt attccaagtt gactacaagt agtcaaggtg
     8461 atataatata tatatcaaga aaggagaaac aaaaatgtat aacgtaggag atcaaataga
     8521 tgagatcgta aaaataggta atgttaaact tgttaaaagt aagtactata tttcagaaac
     8581 tgaaataatt tttaatactg aatcaggatg tattctaatt tggaaactgc cttttcccaa
     8641 agacgaaaca acactgaaaa aaataaatac tttaaaaatc agatattgca ttcatgaaat
     8701 tattgataaa aatacttatg ttataaatca agtaaaagtg atggggtaaa aatcttttaa
     8761 aaaaactctt gttaaaagta attctattag cttataatgt tggtattagt tctgaagcca
     8821 aaggaacact aaaaaattct agtagaaaag ttagttacag actctctact atgtatgaat
     8881 gtaacgaaaa aaatagcgaa aaagataatt aaataagact accaatctta tttaatggaa
     8941 cttctgtagt aagaaaagcg atttggcaat ccacgaagtc caccaagatg agctaccaac
     9001 tcattttaat ttttgcacgt ttaaagttta gcatttaagt gcttactcta aagacatagt
     9061 gtaattttag gactccttat tacggaatct aaagttaccc tatgtctttt ttgctattca
     9121 aaattgatag caaggagaaa gtgcaatgtt gaatagttgc gagtatgcgg acgttttagc
     9181 taaaattctt aaaaatggaa ttaaacagtt taatagcaaa aatctagttg cttttaaaaa
     9241 gaatagcaat aagattggtg ctattgccgg atttacttct aaaacaaaca tgataaagac
     9301 taaaggtctt atatatcgtt ctattgatcg tgtcttggaa gattcagatg tattaacaca
     9361 ctggactcct aacgtgtatt catggcttgg tggcggtgaa aacaaaacaa tttttggtca
     9421 caaagaagct aacttaattc agattaatac ttttgtagca gatattgact atccagtagg
     9481 agaaaagaaa acggatttga atttactagt tctttatcta ttggaagaag ggttattgcc
     9541 tacactgatt gtagatacgc caaaaggtta tcattgctat tttctgattc aaaactataa
     9601 ccaagaaact gattcatttg ataaggcaag ctatatttct aattcaaatg attttaaatc
     9661 tttgagagtt gctaaaagaa tatcagaaaa tatcagaaaa tctatccaaa atcgtttacc
     9721 acaagtcgat atgggctgta atcattttgg tatttttaga tttccaacta aaaaaaatat
     9781 tgtacattat gaacctaatt tcgtagatac gtttgaaggg tatttgaaat ggtcaaaaga
     9841 ctttgaagaa aaagaatatg ctttaaagag gtctaacttg tcattattag ctcctaattc
     9901 tactggcaag aaagggtcta aacgacaaat tgatactaaa tggtacgact ttttaattca
     9961 tacggatatt gctgaaggag atagaaataa aactattttt accttagccc tggcatgtaa
    10021 atcaagtggc ttatctattg gcgagtgtat ggatcaattg gatcaacttc aattcaataa
    10081 tgatttgtcc tattctgaag ttgagagaac aataagaagt gcttatcgtg gcgattacaa
    10141 aggtgctaaa agttcatatg tcaatgaaat tatcgaaaat tatgctacac ctaccgagtt
    10201 tgaaaaatac tcaataaatg taaatagaca agctgttaaa cgtaaaggga atcgtgtcga
    10261 tccgtctaat tgggtgaaat ttgctaaacc tagagaagaa cgaaaatata gtcattttga
    10321 agaaagtaaa gctgatttat tagcttactt agaaagaaaa caaagatcat tgaaaaaaaa
    10381 tgagttgttt ttaagtgtct ctatgggaac tatatctgaa gaaacacaaa ttcctgtatc
    10441 tacgttaaag gtgattttaa aagagcttag aacggcgaat acagtgatat tacgaacgtc
    10501 tcgtggtcgg tatggatcga ctaaaatagc aacaagcaaa tttgtggaag ctaaaatatt
    10561 aaatacagtt attcagaata aaaaagataa acaagtattt gtcttagaat tattaggaac
    10621 agcaaatagc cacgtagaaa cgattttaag acgttatgaa gacaaaacag taccgttcca
    10681 tgcgaacata ctgaaagaaa gagaaaagag caaacatgcg ccaattagag gagctaatac
    10741 aggttagagt tctatttatt tttttgattt ttaaatagct aaattgccaa ttaacatatc
    10801 tagtgtttct gcccactcct attcctgttt tagcgtatac taatgattag tagttagtac
    10861 tctcttttat gtatggagtg tatagaacta tttttacttg tgtcttttcc cttaatccac
    10921 gtgtacggaa tacagcttat aactgcgtat gcagttatgt ttgaaggagg aagattttct
    10981 ttggtaatta agaaatatgt ttactcacac ccgatcaatg tatttattat cggtaagcta
    11041 ggtttatctg ttgaggtgtt ttgtgagtta tatggattta aacaaggaac tctatcgtct
    11101 tggattacta gagagcgtga ggtagctgat ttgcctattt cttttttgta tgctttgtct
    11161 ctagcttcag gagggaatat gaatgaggtt tatgattgct tgtgtgagta tgaaaaagag
    11221 tataaagagt atttgaaaat ttcagcccca aaacggaata aaaaatatat tgaagattag
    11281 agaaagaaga aaagaataat ttgttttttg actacaagta gacagatagc tactctgttt
    11341 tttgatagtt tgatattggc tgtttttttg ttagaattaa tatatatcag gtaaataaaa
    11401 aagggggcta ttaatgaata ttgatgggat ttataatgaa tcagatttta aaaaaggcat
    11461 agtagatatt gatagaaaag agtatttatt tcctgatgaa ataggtttat ttaaaattgt
    11521 aggtgaaact gtctgtaggt attgggggaa gaaaggaaat ataattttac ttgttgatac
    11581 gattgaggga agaaagttaa aattttcgtt ttggagtaat acaaaatatt gttgtgaacc
    11641 tgtacctaaa agggaagtag atgtggctaa agaaagtgta cgatttttac ctgatcgatc
    11701 aaaaataact ttaatttgtg aacggacaag taatgctaaa tttacaagaa ttagagatat
    11761 actactttcg actacaagta gtcagtcata gagtaagaac ttttggttaa aaatcaattg
    11821 aagaaagtgg aggaagtata tggcagtagt tatattagca gaaaaaccag atcaaggagc
    11881 aaaatttgct tcagcattgg ccggaaaaaa attatcgaaa aagagtggga agtatgaatt
    11941 taaaagtgaa atatttggag atactattgt tacttgggga ataggtcatt tggtaggatt
    12001 atcattacct gaaaagtatg agtggctacc gaataaagat aaatggcatt tagataattt
    12061 gccttttcta cctaaattca atgaattaaa gtatgaagtc tctaaaggaa aaaaccaaca
    12121 attttcaata gtgaagtcat gtttagaaaa tgcagatgag ataataattg ctactgatcc
    12181 ggatagagaa ggagaaaata ttgcttataa tattttcaaa ttatgcaagc agtcgatttt
    12241 taataaaccg ataaaaagat tatggattaa ttctttggtt caaaaagaaa ttatcaaagg
    12301 atttaaaaat ctgcgaaatt ctgaagaaac gatctcttac ttcaaagaag ctaatgctag
    12361 acaaattgct gactatctag tagggatgaa ttatacagag ttgtattcat taaagttgca
    12421 agaaaaaggt atatcgggtg tggtatcact cggtcgagtt cagacaccgg taaacacact
    12481 tgtggttgaa aacgatttgg caattagaaa ttttagaccg gaaaaatata aaacgattga
    12541 atgtcataca accgataagc aaccaaaagc aatatttaag aatagtcttg aatactttaa
    12601 attagaaaaa tttgaagaag acactaaaaa attcggcctt gattccgcca gtactggaat
    12661 cataaaaaaa attgaggtta gtaaaaagca aacagaacca caaaaattgt ttagcttggg
    12721 tggtattcaa gaatacgcta ataaaaaatg gaaatattct gctaaaaaaa cagataaaat
    12781 tatccaaagt ctatatgatc gtggatattt atcttatcca agaaccgata gtgaattgat
    12841 aactacaaat gaatttgagt atttaaaagc gaatttagac gtgtataaga gtgttttagg
    12901 gatcagtatt gatacacctt gtttagagcc taaaaagcgg tttgtagata atgacaaagt
    12961 attagagcat tatgcactaa taccaacaat ggatattcct gatatagcaa atctgacatt
    13021 agatgaaaaa aatatttatg agatcgttac taggcgtgcg tgtctaatgt ttgcagaacc
    13081 ttacaagtac gaaaatacaa aagttacatt agatgtaaac ggcttagaat ttaccacaag
    13141 tggtaatgta cctatatctt taggttggaa gcaagtagat actaatttag atgaatctga
    13201 tgataaagag gaaacattac ctaaatttca tgaacaagag acaataaata tagatattag
    13261 atttattgat aaattaacta aggaacctaa aaggttgact gaaggtaaat tattaggtaa
    13321 aaccggtatt atggcgaaat tagggctagg aacgcccgct acacgttcta atattgtaga
    13381 cactcttcta aatagggaat atattaagat agaaaacacg aatgtttatc ctactccaaa
    13441 gggatattta ttatgggatt tgacaaagaa taaagaccta ttgattggaa aaccagaaaa
    13501 tacagcgata tgggaagaat cacttgggaa aattgcgcaa ggtagctata cacaggaagc
    13561 ttttttaaga aatatctata actatatcaa taagactgtt tcagatttga aaaaagtaga
    13621 atttacttcg gattatttag aaaagactgt aaatcaaaat aaatttgata taggtagtta
    13681 tcaagtggaa gaaaagaaaa aagtttattc tattttaaat attactagtg gtgaacagtt
    13741 cgtgatattt aaaaatatat caggaaaagc aatcaataaa aaagttcttc aagaactatt
    13801 actaaataat cgtacagata aagtcctttc cggctttaag tcaaaagaag gaaaagcttt
    13861 taatgcaaga ttacttttcg atccaaatgt gatgaaagtg gtaatggagt ttgaaaagaa
    13921 cagtccaaaa ggcgataaaa agagcaaaca cgatacagta aatggttatg aaatagttga
    13981 aaaagcaaaa gtatttgaaa taaaagaatt agcaacaggt gaaagtttta ttttctataa
    14041 aaataatagt gggaaaactt taagttcaaa aacagtcaaa gaattgttag aaaatgggaa
    14101 aactaaagag aaagtaacgg gttttataag taaagacaat aaaaaatata gtgcttatct
    14161 tgtctttgat aataaaaaca aagttataaa gaaagaattt taattcttag tggcggaaat
    14221 catttgtttt tccgcttatt tttattcaaa agtgcccaaa atataggtat actgaattaa
    14281 ggataaatac aaaactagtt agaggacagt tgaaaaaata caaacaaatt agattgctat
    14341 tttgttaaat tggaagtaga ataataccgt tttgatatgt atagtgtgtt ttaccaata
//
LOCUS       DACEND010000310        10623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.094, whole genome shotgun sequence.
ACCESSION   DACEND010000310 DACEND010000000
VERSION     DACEND010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.094"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            211..1107
                     /locus_tag="IW746_002825"
     CDS             211..1107
                     /locus_tag="IW746_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1079680.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1257..2198)
                     /locus_tag="IW746_002826"
     CDS             complement(1257..2198)
                     /locus_tag="IW746_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1079681.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2324..3445
                     /locus_tag="IW746_002827"
     CDS             2324..3445
                     /locus_tag="IW746_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1079682.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3703..5205
                     /locus_tag="IW746_002828"
     CDS             3703..5205
                     /locus_tag="IW746_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1079683.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5272..5799
                     /locus_tag="IW746_002829"
     CDS             5272..5799
                     /locus_tag="IW746_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1079684.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6010..7071
                     /locus_tag="IW746_002830"
     CDS             6010..7071
                     /locus_tag="IW746_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1079685.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7074..7970
                     /gene="murQ"
                     /locus_tag="IW746_002831"
     CDS             7074..7970
                     /gene="murQ"
                     /locus_tag="IW746_002831"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1079686.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7984..9966
                     /locus_tag="IW746_002832"
     CDS             7984..9966
                     /locus_tag="IW746_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1079687.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10031..10477)
                     /gene="nrdI"
                     /locus_tag="IW746_002833"
     CDS             complement(10031..10477)
                     /gene="nrdI"
                     /locus_tag="IW746_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1079688.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3476 a         1866 c         2287 g         2994 t
ORIGIN      
        1 atttaacaat gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt
       61 aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt
      121 tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga
      181 aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca
      241 aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa
      301 gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg
      361 atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc
      421 aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg
      481 aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca
      541 aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct
      601 agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca
      661 gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca
      721 catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg
      781 gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca
      841 tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat
      901 catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg
      961 gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca
     1021 gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat
     1081 gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa
     1141 acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt
     1201 gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat
     1261 tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt
     1321 gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca
     1381 gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg
     1441 tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct
     1501 tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct
     1561 ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt
     1621 ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt
     1681 aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc
     1741 aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga
     1801 ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt
     1861 acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg
     1921 gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag
     1981 tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc
     2041 acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca
     2101 ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg
     2161 ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa
     2221 tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa
     2281 atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc
     2341 aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg
     2401 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
     2461 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
     2521 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
     2581 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
     2641 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
     2701 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
     2761 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
     2821 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
     2881 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
     2941 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
     3001 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
     3061 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
     3121 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
     3181 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
     3241 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
     3301 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
     3361 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     3421 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     3481 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     3541 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     3601 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     3661 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     3721 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     3781 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     3841 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     3901 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     3961 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     4021 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     4081 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     4141 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     4201 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     4261 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     4321 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     4381 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     4441 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     4501 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     4561 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     4621 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     4681 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     4741 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     4801 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     4861 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     4921 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     4981 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     5041 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     5101 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     5161 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     5221 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     5281 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     5341 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     5401 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     5461 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     5521 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     5581 gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt
     5641 cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat
     5701 gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca
     5761 ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa
     5821 ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc
     5881 ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg
     5941 aaattttgtt tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag
     6001 agggataata tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa
     6061 cagtatatcc gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata
     6121 ccagaagatg atgtttcggc atatcaaaag cgattgatcg aactaggaag cgaagcgaaa
     6181 aagaataaac tagaattgat ggtcgatatt tcaggagagg cattggaacg agcgggtttt
     6241 tcattagaag atctaacgcc tttgaaagca attggtgtaa caggtttaag aatggattat
     6301 catatttcca atcagcagat tgccgactgg tcacatcaat tgaaaatcag tttgaatgct
     6361 agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga tttctcacag
     6421 attgaagctt ggcataatta ttatccacgt cccgaaacag ggctggacaa agaatggtat
     6481 cagaaaaaaa atcaatggct caaaattcaa ggtctccttg tccaaggttt tgtaccagga
     6541 gatacagagt tgagaggccc actatatcaa ggattgccaa ctttggaaga acatcgaggg
     6601 atccatcctc ttgcaggcgc tctagatcta ttagcaagta acacggatat tgtttacatc
     6661 ggagatgcag gtctgtcaga aaacgttcag gagcaatttg cttctttcca aaaagaacag
     6721 acagtcttgc tccatacgga acctgtagat gaagaatttt acgaatatat attagggaag
     6781 catacgaatc ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc acgttttcga
     6841 gagattccgc ctatcccagc aagaaataca gctacgcgta tgaaagggtc tattacttta
     6901 gataacgaaa agtatttgcg ctatatgggt gagatccaat taaccaaata tgatttgcca
     6961 gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag aagagctacc attgatcaac
     7021 caaatcaaag ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca
     7081 aaatcaattt agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg
     7141 agatgagtat ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa gtggccgctg
     7201 cggttgaaaa agaactgaca agtatcgaac cagtgattgc taacacgatt gagtccttta
     7261 aaaaaggtgg acgtctgatt tatatgggag ccggaacaag tggaagacta ggtgtgttag
     7321 atgctgctga atgcgtaccg acattcggtg tagaaccgga aatggtcata ggtctaattg
     7381 ctggtggaga agaagcaatg actgtagctg tcgaaggggc cgaagattct tatgagctag
     7441 ggcaagaaga tctaatcaag ctgaatttga caaaaaatga tatagttatt ggtatcgcag
     7501 ctagcggtcg tacaccatat gtgatcggtg gattagatta cgcaaaatcg atcggtgctg
     7561 tcacaggaac gatttcttgc aataaacaag ccgaaatcag taaacacgcg gatttcccaa
     7621 tcgaagtcga tgcaggtcca gaatttttga caggctctac tcgattgaag tcaggaacag
     7681 cacaaaaact tatcttgaat atgatttcta ctatcagtat gattggtata ggtaaagtat
     7741 tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac
     7801 gaatcatcat gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac tttgaagaag
     7861 cagatcaaaa tgttaagctg gctatcgtca tgattttgac aaatagtgat aaagaggaag
     7921 ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg gaccttatga gggggaaaag
     7981 accatggcaa aaaacgaact gactttggaa gaactggcaa aacaaattta taaaggggca
     8041 ggcggcatgg gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc
     8101 atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta
     8161 gtagaagatg aacagcttca agtcatcatc ggacctggga aagtgaacaa agtagcacag
     8221 caaatggttg atttggcagg cgtgaaatta ggagaaaaac tttcttctgt aagtacagct
     8281 acagcaaatt catcagcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa
     8341 gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa agacatttct
     8401 aatatctttg tgccaatgat tcctgcattt gtcggtactg ggatcgtggc agggattgcc
     8461 gcagttttag caaacttagt cacagccgga acacttgatg cagcaacatg gcaacaatat
     8521 atcgatgtga tgaacatctt gaaaaacggg atgtttagct acctagttat ctatacaggt
     8581 atcaatgctg cacaagtttt tggtgcaaca ccaactcttg gtggagttat cggggccgtt
     8641 gtgatgctga cagggatgaa tccggaagca ccactgaaaa acttatttac tggtgaagct
     8701 cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct attgtcaatc
     8761 gttgagaaaa aactgcataa agtagtacct gatgcggtag atatcatcgt tacaccaact
     8821 ctttcgttga ttgccatcgg tctgattgaa attttcttca tcatgccttt agcaggtttc
     8881 atttctgatg gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg agcatttgcc
     8941 ggcttcgttc taggcacatt attcttgccg atggttatgt tcggactgca tcaaattttg
     9001 actccaatcc atgtgcaaat gattgatgaa actggtcgta cgctattgct tccaattctt
     9061 gcgatggctg gtgcaggaca agtgggtgca gcattggcat tatggattcg ctgcaaaaaa
     9121 gataaagaat taacagaaat gatcaaagga gctttaccag tcgggatcct aggaatcggc
     9181 gaaccattga tttacggagt aacattacct ctaggacgcc catttattac tgcttgtatc
     9241 ggtggaggta tcggcggggc agtgatcgga atgatcggaa atgtcggggc tatcgctatt
     9301 ggaccttcag gagccgcttt gatcccattg atctctgacg gtaaatggtt aggctacatc
     9361 cttggattat tggcagctta tgctggtggc ttcgttgcta cattcttctt cggtattcct
     9421 aaagaccgtt tagaaaaaga agaattagcg actgaaacag ccacagtaaa tgaagtgcct
     9481 gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta tcctctcatc agttgctgac
     9541 ggaacagttg tttctttaga taaagcaagt gatccagttt ttgcacaaaa gatgatgggt
     9601 gaaggctact ttgtagaacc agaaaacgga catatctatt ctccagttac tggggtagtc
     9661 agctctgtct tcccaacgaa acacgctatt gggatcacaa cgccaaatgg tttagaaatc
     9721 ttgcttcata tgggaatcaa cacagttgac ttaggcggta cgccatttga tgtgaaagta
     9781 acagaagggc agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcgatc
     9841 aaagcagcag gcaaagaaac atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa
     9901 aactttgtct tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa
     9961 gcttaatatg atagaaacaa tagaaacgaa agaagaaact gaaatctatt tttcagcttc
    10021 ttctttttta ttatttattg ttttctgcta aaatctgata aattcgtgtc acatcagatg
    10081 gtgttccccg taattcataa tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt
    10141 tggctgttag acaatactga ttattgaagt ttttatttcc gctaccgact acaccaagac
    10201 aaagtttatg attgtcttca tgttccagat attcacgcat cgtttcggtc aagatttctg
    10261 tatcaccgtt atctaaacca tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg
    10321 gttctgtttc tttcgtaaaa tcactatttt catggatttc ttttaaagta acttcaggat
    10381 tgacaggatc ttctgcatgc attttttcag catattctgc tagatgtttg gcaaaggcac
    10441 gcgtatttcc tgaaatcgat atatataaaa tgttcatgga tcatcctcca attctttcct
    10501 tttagtatag catctttttt aagttttttg gtggtgtaat ttggaaaggg actaggcgct
    10561 ttgcttgtat aatgagaaaa aagccggtag aattatattt ctattaaagt ttttattaat
    10621 taa
//
LOCUS       DACEND010000311         6749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.134, whole genome shotgun sequence.
ACCESSION   DACEND010000311 DACEND010000000
VERSION     DACEND010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.134"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            111..194
                     /locus_tag="IW746_002834"
     CDS             111..194
                     /locus_tag="IW746_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1079689.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IW746_002835"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IW746_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1079690.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="IW746_002836"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="IW746_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1649..2314
                     /locus_tag="IW746_002837"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="IW746_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="IW746_002838"
     CDS             2350..2595
                     /locus_tag="IW746_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1079691.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="IW746_002839"
     CDS             2698..3567
                     /locus_tag="IW746_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1079692.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="IW746_002840"
     CDS             3548..4282
                     /locus_tag="IW746_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1079693.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5227
                     /gene="ant(6)"
                     /locus_tag="IW746_002841"
                     /pseudo
     CDS             4315..5227
                     /gene="ant(6)"
                     /locus_tag="IW746_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5224..5766
                     /gene="sat4"
                     /locus_tag="IW746_002842"
     CDS             5224..5766
                     /gene="sat4"
                     /locus_tag="IW746_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1079694.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5859..6653
                     /locus_tag="IW746_002843"
                     /note="aph(3')-IIIa"
     CDS             5859..6653
                     /locus_tag="IW746_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1079695.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2365 a         1015 c         1407 g         1962 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgaacgtat
     4381 tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag atgaatttca
     4441 ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta atgatgactg
     4501 gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg aattattccc
     4561 acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata aaattgatct
     4621 taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat taataaaggt
     4681 tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata tagattatca
     4741 tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt ggaatgtaac
     4801 accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg atcattttaa
     4861 tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca tcgaaacagg
     4921 ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat ccgaggattt
     4981 gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat gggaagcatt
     5041 atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa ggcttcatta
     5101 tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt ataaaaaata
     5161 cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag agaggcggga
     5221 acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc cagcgaacca
     5281 tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa
     5341 ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag
     5401 gaggcagatt gccttgaata tattgacaat actgataaga taatatatct ttactaccaa
     5461 gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta cgcttatata
     5521 gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat
     5581 atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac
     5641 aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat
     5701 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     5761 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     5821 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     5881 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     5941 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     6001 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     6061 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     6121 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     6181 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     6241 cggattgtcc ctatacgaat agcttagaca gccgattagc cgaattggat tacttactga
     6301 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     6361 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     6421 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     6481 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     6541 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     6601 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     6661 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctggctc
     6721 tttgtcaaat gatgttggta aagagaaat
//
LOCUS       DACEND010000312         5887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.141, whole genome shotgun sequence.
ACCESSION   DACEND010000312 DACEND010000000
VERSION     DACEND010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.141"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            406..870
                     /locus_tag="IW746_002844"
     CDS             406..870
                     /locus_tag="IW746_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1079696.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            854..1483
                     /locus_tag="IW746_002845"
     CDS             854..1483
                     /locus_tag="IW746_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1079697.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1837..3315
                     /gene="msr"
                     /locus_tag="IW746_002846"
     CDS             1837..3315
                     /gene="msr"
                     /locus_tag="IW746_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1079698.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3362..3808
                     /locus_tag="IW746_002847"
     CDS             3362..3808
                     /locus_tag="IW746_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1079699.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4007..4522)
                     /locus_tag="IW746_002848"
     CDS             complement(4007..4522)
                     /locus_tag="IW746_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1079700.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4639..5841
                     /locus_tag="IW746_002849"
     CDS             4639..5841
                     /locus_tag="IW746_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1079701.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1820 a          984 c         1242 g         1841 t
ORIGIN      
        1 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
       61 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
      121 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt ttctactgca
      181 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
      241 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
      301 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
      361 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
      421 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
      481 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
      541 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
      601 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
      661 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcgagca ttgtgaaatg
      721 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
      781 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
      841 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
      901 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
      961 agaagcgacg actgcacctt cttctgtcaa tatgcaacca tggcgctttg tggtcgctga
     1021 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
     1081 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
     1141 aatctataac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
     1201 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
     1261 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
     1321 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
     1381 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
     1441 gtctgttcgt ttagcaccag aaaagatcac tacgttcgaa taaaaggttt tcagttacta
     1501 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
     1561 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
     1621 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
     1681 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
     1741 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
     1801 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
     1861 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
     1921 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
     1981 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
     2041 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
     2101 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
     2161 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
     2221 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
     2281 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
     2341 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
     2401 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
     2461 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
     2521 gtgtccagta aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa
     2581 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     2641 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     2701 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     2761 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     2821 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     2881 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     2941 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     3001 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     3061 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     3121 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     3181 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     3241 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     3301 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     3361 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
     3421 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
     3481 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
     3541 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
     3601 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
     3661 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
     3721 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
     3781 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
     3841 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
     3901 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
     3961 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
     4021 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
     4081 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
     4141 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
     4201 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
     4261 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
     4321 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
     4381 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
     4441 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
     4501 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
     4561 acagtaaagt ttccacgttt tacatgtgca tatttacatg taaaataaat catgtatgat
     4621 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
     4681 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
     4741 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
     4801 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
     4861 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
     4921 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
     4981 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
     5041 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
     5101 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
     5161 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
     5221 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
     5281 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
     5341 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
     5401 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
     5461 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
     5521 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
     5581 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
     5641 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
     5701 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
     5761 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
     5821 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
     5881 ataagaa
//
LOCUS       DACEND010000313         5238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.150, whole genome shotgun sequence.
ACCESSION   DACEND010000313 DACEND010000000
VERSION     DACEND010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.150"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            16..1902
                     /locus_tag="IW746_002850"
     CDS             16..1902
                     /locus_tag="IW746_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1079702.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            1914..2861
                     /locus_tag="IW746_002851"
     CDS             1914..2861
                     /locus_tag="IW746_002851"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1079703.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2881..3408
                     /locus_tag="IW746_002852"
     CDS             2881..3408
                     /locus_tag="IW746_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1079704.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3471..4124)
                     /locus_tag="IW746_002853"
     CDS             complement(3471..4124)
                     /locus_tag="IW746_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1079705.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4256..5098
                     /locus_tag="IW746_002854"
     CDS             4256..5098
                     /locus_tag="IW746_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1079706.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         1869 a          910 c         1109 g         1350 t
ORIGIN      
        1 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
       61 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
      121 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
      181 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
      241 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
      301 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
      361 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
      421 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
      481 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
      541 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
      601 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
      661 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
      721 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
      781 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
      841 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
      901 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
      961 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     1021 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     1081 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     1141 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     1201 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     1261 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     1321 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     1381 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     1441 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     1501 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     1561 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     1621 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     1681 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     1741 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     1801 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     1861 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     1921 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     1981 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     2041 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     2101 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     2161 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     2221 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     2281 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     2341 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     2401 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     2461 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     2521 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     2581 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     2641 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     2701 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     2761 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     2821 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     2881 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     2941 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     3001 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     3061 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     3121 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     3181 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     3241 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     3301 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     3361 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     3421 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     3481 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     3541 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     3601 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     3661 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     3721 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     3781 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     3841 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     3901 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     3961 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     4021 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     4081 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     4141 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     4201 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     4261 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     4321 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     4381 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     4441 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     4501 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     4561 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     4621 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     4681 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     4741 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     4801 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     4861 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     4921 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     4981 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     5041 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     5101 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     5161 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     5221 aacattagaa ccaatcga
//
LOCUS       DACEND010000314         3297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.181, whole genome shotgun sequence.
ACCESSION   DACEND010000314 DACEND010000000
VERSION     DACEND010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.181"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW746_002855"
     CDS             245..1213
                     /locus_tag="IW746_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1079707.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW746_002856"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW746_002856"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1079708.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW746_002857"
     CDS             2243..2851
                     /locus_tag="IW746_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1079709.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          980 a          632 c          795 g          890 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag gctctatact ttttgatgtt ggtggatgac accagca
//
LOCUS       DACEND010000315         2969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.191, whole genome shotgun sequence.
ACCESSION   DACEND010000315 DACEND010000000
VERSION     DACEND010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.191"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            28..939
                     /locus_tag="IW746_002858"
     CDS             28..939
                     /locus_tag="IW746_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1079710.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1092..1577
                     /gene="vanZ"
                     /locus_tag="IW746_002859"
     CDS             1092..1577
                     /gene="vanZ"
                     /locus_tag="IW746_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1079711.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1864..2193)
                     /locus_tag="IW746_002860"
     CDS             complement(1864..2193)
                     /locus_tag="IW746_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1079712.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2498..2827
                     /locus_tag="IW746_002861"
     CDS             2498..2827
                     /locus_tag="IW746_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1079713.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2817..>2969
                     /locus_tag="IW746_002862"
     CDS             2817..>2969
                     /locus_tag="IW746_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1079714.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSG"
BASE COUNT         1063 a          419 c          523 g          964 t
ORIGIN      
        1 actatatagt taaggaggag actgaaaatg aagaagttgt tttttttatt gttattgtta
       61 ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca ggaaaaagtc
      121 gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg gacttctgaa
      181 aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct gctattaatc
      241 aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa tttatctaaa
      301 catgacgaat taataaatgg atacgggttg cttgatagta atatttatat gtcaaaagaa
      361 atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt tagtcatttt
      421 attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca agaaatgggg
      481 gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc actagatgta
      541 ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga agaaaatgct
      601 tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac aggaattcaa
      661 tatgaaccat ggcatattcg ctatgttggt ttaccacata gtgcgattat gaaagaaaag
      721 aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat ttctgttagt
      781 gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac caccattcat
      841 gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt aattgtgaca
      901 gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa tgaaaccaac
      961 gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc tttttaattt
     1021 aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca aaagaattag
     1081 aggaaattat attgggaaaa atattatcta gaggattgct agctttatat ttagtgacac
     1141 taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt aattatcatc
     1201 aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag atgatagata
     1261 atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa gaaatcggat
     1321 ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata attcaattta
     1381 tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt ggaggctttc
     1441 ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa ttagacagag
     1501 ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt acccatttaa
     1561 gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac acataaagat
     1621 attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg taggtgtgaa
     1681 gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta acaaagaaag
     1741 cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc ttaacgttgt
     1801 aaatccgcat tttcctgacg gtacccccaa actaagattt catagataga ttttctgata
     1861 actttaaaaa acacgaatag cggaaaggat gtcttcattc gctaaagagt aataaactac
     1921 ttttccatct tgacgtgatt ttgccattcc atgttttttt aaaaaacgta aatggtgtga
     1981 cgttgtagcg acactagcag taagtatttc tgctaaatca cagacgcaca tttccttata
     2041 ggtttctaaa gcatagaaaa ttttaattct agaaaaatcg ctgaaacatt tccctaacac
     2101 aagtaagttt gaaaaatctt tttgatccaa tttatttttt attccattta ctttttcttt
     2161 atgtacgaca gtcacttggc aaactttttt cattcatctc acctcacatt caaacgatta
     2221 tttgaatgaa gtatacctat ttgatcactc ttttgtcaat ctaccttaat tccaatacac
     2281 tattataagc aatttcatgt attgaagtaa acttacttgc aagtgcgttt ttgattccaa
     2341 gttaaaaaca tattattatt ctacataatt tcctaatcaa ataaaactcc tttgaaaatt
     2401 gttctaaagg agttttatta ttttgtattt ctactaaaaa tagcgtacaa tgtaattact
     2461 aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa caagaaaaca
     2521 aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg gtgttgaagt
     2581 agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta aaaaagaatt
     2641 ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata gcaaacaaga
     2701 gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta aatctattgc
     2761 tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag cagaaattgg
     2821 cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta aaagagaaaa
     2881 cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt cctcattcgg
     2941 gagaacaaaa aacgggtgat ttatcagga
//
LOCUS       DACEND010000316         2532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.201, whole genome shotgun sequence.
ACCESSION   DACEND010000316 DACEND010000000
VERSION     DACEND010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.201"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            138..1352
                     /locus_tag="IW746_002863"
     CDS             138..1352
                     /locus_tag="IW746_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1079715.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1383..1601
                     /locus_tag="IW746_002864"
                     /pseudo
     CDS             1383..1601
                     /locus_tag="IW746_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2020..2493
                     /gene="dfrF"
                     /locus_tag="IW746_002865"
     CDS             2020..2493
                     /gene="dfrF"
                     /locus_tag="IW746_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1079716.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
BASE COUNT          785 a          393 c          564 g          790 t
ORIGIN      
        1 acgaaaaaga gcacaacaca tcaagctttt attaattgtc tgtcttttat aaatgcaagt
       61 attaattcaa aaaaacagaa gaaaaaaaca actacacctt cttttatact cgtaatcgta
      121 gaaaggaggc gaaaaaaatg aatacattac ctatcaatac aggagataca gcattcatga
      181 ttttatgtac agcaatggtt tgcctgatga ctcctggtct agcttttttt tacggcggat
      241 tagcacggaa gaataatata ctaacgatca tgtctcaatc ttttctctcc gttgggataa
      301 caacgattat gtgggtattt ggtggattcg gattggcctt tggacgggat atcggcggtg
      361 ttattggaaa cccggcagat ttctttttga tgagacatgt tacaagcttc cccaacatgt
      421 ttcatggtgc aacgattcca tttttaatgt tctttcttta ccaactgatg tttatcgtga
      481 ttaccgttcc tttgatgaca ggagcttttg cagggagatt gaatttaaaa ggctatatta
      541 tacttgttat tttttggaat ctgctgattt atttccctgt ttgccactgg atatggggtg
      601 gcggattttt ggatcagatg ggatttagag atttcgctgg tggagcagtc atccacacga
      661 cagctggatt cggctcttta gcatgtatcc tcacacttgg tcaaagaaag attcctttag
      721 gagaaagaag tcagcatagc agtcttatgg cagctgctat cggtacagga ctgctttggt
      781 ttggctggtt cggttttaat tccggtggtg cacttcgagc agatgaacaa gcagtcaatg
      841 catttggttc aacattcatc gctcttgcat ttgcgatgat tacttggctg atcattgcaa
      901 aagtaaaagg aaatggcttt gattttgtcg acgtactaac aggatccgtt gctggattgg
      961 cagccattac gccttgtgcc ggttatgtag aagcaaaaag tgccatgctg attgggatca
     1021 ttgcgggtat cgtctgtcat gctgctgttg attttcgtaa aaagaaacaa tgggatgatg
     1081 cacttgatgt gtggggtgtt catggcatgg gcggattcac aggtacgatt ctgataggga
     1141 tctttgcatc taacagccat ttactaacga atccaagcag ctggtacttc ttagggattc
     1201 aagtgattgg tgtcatcatt actgccgtgt atgcctatgt gctgacaaca gtgattttaa
     1261 aatcggcgat gcactttaca acgatcacta ctacaaaaga agaacaagaa gaaggattgg
     1321 actttttctt ctttggcaac aaactcaatt gaaacaaatt caaatagaaa agaggaagat
     1381 tcatgcaaca aagcaaaacg aaaattcctg caaaaaaatt aacgttcttt ggctttttgg
     1441 ctatgaccat ctcaatggtc gtttcattat atgaatatcc cacattcgcg acctcaggct
     1501 tcagcttagt atttttcttg ttattaggcg gtttgctatg gtttatccct gtggcattgt
     1561 gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa acaaaaagat
     1621 attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta ctagactaag
     1681 agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc agactatttc
     1741 atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc ttgttgcgtg
     1801 agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt tcaaaagcag
     1861 aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca aaaaaggaat
     1921 ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg cacttgatgg
     1981 aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt gattgttgcg
     2041 aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat aaagggagaa
     2101 caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg aaagtcttat
     2161 gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac cacaacagag
     2221 tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattatt attggctaaa
     2281 ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt gcaaatagta
     2341 gataaaatgt atatcacaga agtagattta aatattgaag atggagatac attctttcca
     2401 gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga ggaagtgaaa
     2461 tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga tttagggtgt
     2521 tttcataaat at
//
LOCUS       DACEND010000317         2246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.213, whole genome shotgun sequence.
ACCESSION   DACEND010000317 DACEND010000000
VERSION     DACEND010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.213"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..152
                     /locus_tag="IW746_002866"
     CDS             <1..152
                     /locus_tag="IW746_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1079717.1"
                     /translation="YLIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
     gene            366..1061
                     /gene="vanR"
                     /locus_tag="IW746_002867"
     CDS             366..1061
                     /gene="vanR"
                     /locus_tag="IW746_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1079718.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1039..2193
                     /locus_tag="IW746_002868"
     CDS             1039..2193
                     /locus_tag="IW746_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1079719.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          758 a          410 c          475 g          603 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa ttatgcggta aagctatata
       61 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
      121 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
      181 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
      241 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
      301 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
      361 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
      421 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
      481 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      541 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      601 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      661 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      721 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      781 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      841 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      901 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      961 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
     1021 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
     1081 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
     1141 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
     1201 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
     1261 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
     1321 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
     1381 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
     1441 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
     1501 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
     1561 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
     1621 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
     1681 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
     1741 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
     1801 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
     1861 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
     1921 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
     1981 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
     2041 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
     2101 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
     2161 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ggctctttgt
     2221 caaatgatgt tggtaaagag aaatct
//
LOCUS       DACEND010000318          896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E341
            SAMN07141432-rid9870263.guided.318, whole genome shotgun sequence.
ACCESSION   DACEND010000318 DACEND010000000
VERSION     DACEND010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141432
            Sequence Read Archive: SRR5811435
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740765.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740765.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 01:53:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,869
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 27 of 56
            Pseudo Genes (incomplete)         :: 22 of 56
            Pseudo Genes (internal stop)      :: 16 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141432-rid9870263.guided.318"
                     /isolate="E341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Sep-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            283..831
                     /gene="aac(6')"
                     /locus_tag="IW746_002869"
     CDS             283..831
                     /gene="aac(6')"
                     /locus_tag="IW746_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1079720.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          320 a          129 c          186 g          261 t
ORIGIN      
        1 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
       61 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
      121 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
      181 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
      241 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
      301 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
      361 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
      421 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
      481 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
      541 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
      601 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
      661 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
      721 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
      781 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
      841 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttactt
//
